CN113735651A

CN113735651A - Soil remediation improver and preparation method thereof

Info

Publication number: CN113735651A
Application number: CN202110990451.4A
Authority: CN
Inventors: 张洪富; 曲丽君; 张见; 李欣屹; 李萍; 邢军
Original assignee: Heilongjiang Guxinyuan Fertilizer Co ltd
Current assignee: Heilongjiang Guxinyuan Fertilizer Co ltd
Priority date: 2021-08-26
Filing date: 2021-08-26
Publication date: 2021-12-03

Abstract

The invention discloses a soil remediation improver which is a novel soil fertilizer containing biological strains. The biological strains are composed of various probiotics, so that beneficial floras in soil are greatly increased, the diversity of soil microbial communities is restored, the air permeability is enhanced, the soil hardening problem is effectively solved, and the soil micro-ecological environment is improved. The organic compound fertilizer, the inorganic fertilizer and the microbial culture are combined for use, so that the defect of a single type of fertilizer can be overcome, and the method has important significance for restoring soil fertility, improving the quality and quantity of crops and reducing the using amount of the fertilizer.

Description

Soil remediation improver and preparation method thereof

Technical Field

The invention belongs to the field of agricultural soil improvement, and relates to a soil remediation improver and a preparation method thereof.

Background

In the agricultural production process, in order to increase the yield of crops and maximize the economic benefit, a large number of farmers use a large amount of chemical fertilizers, and abandon the organic fertilizers, and seriously neglect the benefit of recycling the soil, so that the quality of the soil is increasingly poor, and the soil nutrients are seriously lost. The method mainly shows that the fertility of the basic soil is gradually reduced, the content of part of nutrients in the soil is greatly reduced, the buffering capacity of the soil is poor, and the physical properties of the soil are greatly reduced. According to statistics, at the present stage, soil nutrients in a plurality of cultivated lands in China are seriously lost, and even medium and trace elements of a part of cultivated lands do not exist. These factors have severely affected agricultural sustainability in our country.

In the current society, the phenomena of small proportion of high-concentration compound fertilizers and large proportion of low-concentration compound fertilizers generally exist in the fertilizer market, the structure of the fertilizer product is unreasonable, and the nutrients in the fertilizer do not reach the standard. In the process of crop cultivation, when many farmers use fertilizers, only the application of nitrogenous fertilizers and phosphatic fertilizers is still considered according to the traditional fertilizer using method, and the application of microbial nutrient fertilizers is seriously ignored. Thus, the crops can not absorb enough nutrients during the growth process, resulting in poor harvest and yield reduction. In addition, the phenomenon that the soil is not fertilized timely is one of the factors for reducing the soil fertility, and soil nutrients cannot be supplemented, so that the salinization speed of the soil is accelerated, and the recovery capability of the soil is damaged.

The soil restoration modifier is a novel soil fertilizer containing biological strains. The biological strain has the characteristics of rapid propagation, strong vitality, safety, no toxicity, high temperature resistance, no inactivation in the granulation and drying processes, long-term storage and the like, contains abundant and high-value active bacteria and has the effect of improving the soil structure. The biological strains are composed of various probiotics, so that beneficial floras in soil are greatly increased, the diversity of soil microbial communities is restored, the air permeability is enhanced, the soil hardening problem is effectively solved, and the soil micro-ecological environment is improved.

The bacillus subtilis produces active substances such as subtilin, polymyxin, nystatin, gramicidin and the like in the growth process of the bacillus subtilis, can inhibit the breeding of germs and pests in soil, and provides a good environment for the healthy growth of crop roots. And the environment is not polluted, a plurality of antibiotics and enzymes can be generated, the stress resistance of crops can be improved, and the occurrence of crop diseases can be reduced. And the bacillus subtilis is used to show the stimulation effect on the soil respiration, and the larger the mass fraction of the bacillus subtilis in the soil is, the larger the stimulation effect on the soil respiration intensity is, namely, the stimulation intensity is in positive correlation with the pesticide application mass fraction.

Candida utilis can decompose potassium and silicon in minerals and phosphorus in apatite, has the functions of dissolving phosphorus, releasing potassium and fixing nitrogen, and can produce organic acid, amino acid, polysaccharide, hormone and other matters favorable to plant absorption and utilization during growth and propagation. The bacterium secretes plant growth hormone and various enzymes after being propagated in soil. After the dead bacteria, the potassium in the bacteria is dissociated and can be absorbed and utilized by plants. As an important functional bacterium in microbial fertilizer, the fertilizer can improve the content of quick-acting potassium and phosphorus in soil, improve the yield and quality of crops and the like.

The rhodopseudomonas palustris has the effect of promoting the proliferation of soil microorganisms, can proliferate in soil and stimulate the proliferation of azotobacter and actinomycetes in the soil, so that the quantity of beneficial bacteria in the soil is greatly increased.

The organic compound fertilizer, the inorganic fertilizer and the microbial culture are combined for use, so that the defect of a single type of fertilizer can be overcome, and the method has important significance for restoring soil fertility, improving the quality and quantity of crops and reducing the using amount of the fertilizer. Some soil remediation conditioners also appear in the prior art, but the soil remediation effect is hardly satisfactory, so that the development of a soil remediation conditioner for agricultural soil remediation is of great importance.

Disclosure of Invention

This section is for the purpose of summarizing some aspects of embodiments of the invention and to briefly introduce some preferred embodiments. In this section, as well as in the abstract and the title of the invention of this application, simplifications or omissions may be made to avoid obscuring the purpose of the section, the abstract and the title, and such simplifications or omissions are not intended to limit the scope of the invention.

The present invention has been made keeping in mind the above problems occurring in the prior art.

Therefore, the invention aims to provide a soil remediation improver and a preparation method thereof according to the defects of the prior art.

To solve the above technical problem, according to an aspect of the present invention, the present invention provides the following technical solutions: a soil restoration improver and a preparation method thereof comprise,

respectively culturing bacillus subtilis, candida utilis and rhodopseudomonas palustris, preparing fermentation mother liquor according to a proportion, inoculating the fermentation mother liquor into a fermentation substrate to carry out anaerobic fermentation to obtain a microbial culture, adding an organic compound fertilizer and an inorganic fertilizer, and uniformly mixing the organic compound fertilizer and the inorganic fertilizer with the microbial culture according to a proportion to obtain a remediation soil conditioner;

and drying, disinfecting, disinsection, screening and storing the obtained soil remediation improver for later use.

As a preferred scheme of the preparation method of the soil remediation improver, the method comprises the following steps: the respective culture of the bacillus subtilis, the candida utilis and the rhodopseudomonas palustris comprises the steps of pure strain culture and fermentation according to respective culture medium and culture step conditions, and then the pure strain culture and the fermentation are mixed in proportion to prepare a yeast solution.

As a preferred scheme of the preparation method of the soil remediation improver, the method comprises the following steps: the respective culture media, including,

culture medium of bacillus subtilis: 3.0g of beef extract, 10.0g of peptone, 5.0g of NaCl and 20.0g of agar;

culture medium of candida utilis: 30.0g of malt extract, 5.0g of bacterial peptone and 15.0g of agar;

culture medium of rhodopseudomonas palustris: k₂HPO₄ 1.0g，MgSO₄0.5g, yeast extract 10.0g, and distilled water 1000 ml.

As a preferred scheme of the preparation method of the soil remediation improver, the method comprises the following steps: the culturing step comprises

The culture step of the bacillus subtilis comprises the following steps: preparing culture solution, stirring, adjusting pH to 7, and sterilizing at 121 deg.C for 15 min; activating the bacillus subtilis strain, adding a single bacterial colony into a culture solution according to the addition of 10 percent, culturing and inoculating, and stably culturing at 30-32 ℃.

The culture step of the candida utilis comprises the following steps: preparing a culture solution, uniformly stirring, adjusting the pH value to 5.2-5.6, and sterilizing for later use; inoculating the candida utilis culture strain into the culture solution in an inoculation amount of 7-10%, and fermenting and culturing at 28-30 ℃.

The culture step of rhodopseudomonas palustris comprises the following steps: preparing a culture solution, uniformly stirring, adjusting the pH value to 7.0-7.2, and sterilizing at 121 ℃ for 15min for later use; the rhodopseudomonas palustris strain activation is to add a single bacterial colony into a culture solution according to the addition of 10 percent for culture and inoculation, and the culture is stably sealed at 30-32 ℃ and cultured by a tungsten lamp with 40 watts under illumination.

As a preferred scheme of the preparation method of the soil remediation improver, the method comprises the following steps: the fermentation mother liquor prepared according to the proportion comprises 45-70% of bacillus subtilis, 15-30% of candida utilis and 10-30% of rhodopseudomonas palustris.

As a preferred scheme of the preparation method of the soil remediation improver, the method comprises the following steps: the fermentation substrate comprises 15% of corn, 30% of soybean meal and 65% of bran.

As a preferred scheme of the preparation method of the soil remediation improver, the method comprises the following steps: the anaerobic fermentation comprises the following steps of,

inoculating fermentation mother liquor into the uniformly stirred solid fermentation substrate subjected to steam sterilization by a plate marking method according to the proportion of 40%, stirring for 10 minutes, inoculating into a special anaerobic fermentation bag, sealing the fermentation bag in a heat sealing manner, and moving into a fermentation chamber at 25 ℃ for anaerobic fermentation for 8 days; wherein, the anaerobic fermentation bag is that 8 layers of nylon are compound to be inlayed and have one-way discharge valve, and when the gaseous certain pressure that produces of thallus growth breathing reached, discharge valve automatic outwards exhausts, and the gaseous unable entering fermentation bag of outside has guaranteed that the culture is not polluted by miscellaneous fungus.

As a preferred scheme of the preparation method of the soil remediation improver, the method comprises the following steps: the organic compound fertilizer comprises one or more of wet compost, mud fertilizer, stable manure and the like.

As a preferred scheme of the preparation method of the soil remediation improver, the method comprises the following steps: the inorganic fertilizer comprises 50-70 g/kg of nitrogenous fertilizer, 40-60 g/kg of phosphate fertilizer and 60-70 g/kg of potash fertilizer.

As a preferred scheme of the preparation method of the soil remediation improver, the method comprises the following steps: the organic fertilizer and the inorganic fertilizer are mixed according to the proportion, wherein the mass ratio of the organic compound fertilizer to the inorganic fertilizer to the microbial culture is 5: 3: 2.

the invention has the beneficial effects that:

the bacillus subtilis, candida utilis and rhodopseudomonas palustris used in the invention can generate various decomposition enzymes, can decompose potassium and silicon in minerals and phosphorus in apatite, have the functions of dissolving phosphorus, releasing potassium and fixing nitrogen, and can generate substances which are beneficial to plant absorption and utilization, such as organic acid, amino acid, polysaccharide, hormone and the like in the growth and propagation process so as to inhibit the breeding of germs and pests in soil. The fertilizer can also quickly grow in a hardened and compact and anoxic environment, does not generate toxic and harmful substances, promotes the growth of crops, and improves the resistance of the crops.

According to the invention, the organic fertilizer, the inorganic fertilizer and the microbial culture are fused to produce the microbial regenerative fertilizer for restoring, reducing and improving soil fertility, so as to promote rapid and mass propagation and growth of beneficial microorganisms artificially propagated. The beneficial microorganism proliferation and soil active enzyme are greatly improved, the diversity of soil microbial communities is recovered, the air permeability is enhanced, the soil hardening problem is effectively solved, the soil micro-ecological environment is improved, and the soil nutrient content is improved.

Detailed Description

In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with examples are described in detail below.

In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, but the present invention may be practiced in other ways than those specifically described and will be readily apparent to those of ordinary skill in the art without departing from the spirit of the present invention, and therefore the present invention is not limited to the specific embodiments disclosed below.

Furthermore, reference herein to "one embodiment" or "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment is included in at least one implementation of the invention. The appearances of the phrase "in one embodiment" in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments.

The raw materials used in the present invention are commercially available unless otherwise specified.

Wherein, the bacillus subtilis strain is purchased from Beijing Beinanna Chuanglian Biotechnology research institute, and is numbered BNCC 188062.

The Candida utilis strain is purchased from Beijing Beinanna Chuanglian Biotechnology research institute, and is numbered BNCC 185498.

Rhodopseudomonas palustris strain is purchased from Beijing Beinanna institute of Biotechnology, and is numbered BNCC 336448.

Example 1:

carrying out pure strain culture and fermentation on bacillus subtilis, candida utilis and rhodopseudomonas palustris according to respective culture media and culture step conditions, and then, mixing the materials in a mass ratio of 7: 2: 1 mixing to prepare a yeast solution;

inoculating the fermentation mother liquor into the uniformly stirred solid fermentation substrate subjected to steam sterilization by a plate marking method according to the proportion of 40%, stirring for 10 minutes, inoculating into a special anaerobic fermentation bag, sealing the fermentation bag in a heat sealing manner, and moving into a fermentation chamber at 25 ℃ for anaerobic fermentation for 8 days.

Adding organic compound fertilizer and inorganic fertilizer into the fermented microbial culture according to the mass ratio of 5: 3: 2. and (3) uniformly mixing to obtain the soil remediation modifier, and drying, disinfecting, killing insects and storing the obtained soil remediation modifier for later use.

100kg of soil restoration improver is uniformly added into one mu (666 square meters) of non-planting soil with the pH value of 9.5. Random weekly sampling of 1m²Sampling for 25 times, air-drying to obtain an air-dried soil sample, and measuring and then averaging. The data obtained are shown in Table 1.

The concrete operation of measuring the pH value of the soil is as follows: weighing 25g of air-dried soil sample passing through a 1mm pore size sieve, putting the air-dried soil sample into a 50ml beaker, adding 25ml of distilled water, stirring the mixture for 1 minute by using a glass rod to ensure that the soil mass is fully dispersed, and measuring the mixture by using an acidimeter after the mixture is placed for half an hour (the influence of ammonia or volatile acid in the air is avoided).

The invention adopts a hydrated thermogravimetric potassium chromate oxidation-colorimetry to measure soil organic matters, and the specific operation is as follows: weighing 0.5g of air-dried soil sample which is sieved by a 0.25mm sieve, putting the air-dried soil sample into a 20ml hard test tube, adding 10ml of 0.136mol/L potassium dichromate-sulfuric acid solution, and standing for 20 min; preheating a paraffin oil bath pan, heating to 185-190 ℃, inserting a hard test tube into an iron wire cage, putting the iron wire cage into the oil bath pan, heating, keeping boiling at 170-180 ℃ for 5min, and taking out and cooling; and after cooling, washing the solution in the test tube into a 250ml triangular flask to ensure that the total volume in the flask is 60-80 ml, adding 3-5 drops of an o-philone indicator, titrating with 0.2N standard ferrous sulfate, and obtaining a titration end point when the solution is changed from yellow to brownish red through green mutation. The number of ferrous sulfate volumes consumed was recorded. Calculated as follows:

wherein: v₀The amount of the ferrous sulfate used for titrating the blank liquid is milliliter; ml of

V₁The amount of millilitres of ferrous sulphate used in titrating the sample liquid; ml of

N-ferrous sulfate equivalent concentration.

1.1-Oxidation correction constant.

1.724-empirical constant for 1g of carbon to be converted to organic matter.

0.003-milliequivalent of carbon

TABLE 1 remediation of the effects of soil amendment on soil

The soil added with the soil remediation improver obviously improves the pH value, improves the content of organic matters and effectively improves the soil matrix. 4-week observation shows that when the soil conditioner is added to repair crops planted in the soil, the crops grow fast, the leaves are wide, thick and tender, no fallen leaves exist, the color is light green, no yellow leaves exist, and the root system is thick and white; the crops planted in the soil without the soil restoration improver are deciduous leaves and yellow leaves, the stems are not tall and straight enough, the root systems are aged and are in yellow brown.

Example 2:

pure strain culture and fermentation are carried out on bacillus subtilis, candida utilis and rhodopseudomonas palustris according to respective culture media and culture step conditions, and then the pure strain culture and the fermentation are mixed to prepare a yeast solution, wherein the ratio of each strain is shown in table 2.

Adding an organic compound fertilizer and an inorganic fertilizer into the fermented microbial culture, and uniformly mixing to obtain the soil remediation improver, wherein the mass ratio of the organic compound fertilizer to the inorganic fertilizer is 5: 3: 2. and drying, disinfecting, disinsection and storing the obtained soil remediation improver for later use.

100kg of soil restoration improver is uniformly added into one mu (666 square meters) of soil. Random sampling 1m after 4 weeks²The samples were taken 25 times, measured and averaged, and the data obtained are shown in Table 2.

The concrete operation of the invention for measuring soil urease activity is as follows: weighing 5g of soil sample into a 50ml triangular flask, adding 1ml of toluene, uniformly oscillating, adding 10ml of 10% urea solution and 20ml of citrate buffer solution with pH of 6.7 after 15min, shaking uniformly, and then culturing for 24 hours in a 37 ℃ incubator. After the culture, filtering, adding 1ml of filtrate into a 50ml volumetric flask, adding 4ml of 1.35mol/L sodium phenate solution and 3ml of sodium hypochlorite solution (the concentration of active chlorine is 0.9%), and shaking up. Developing after 20min, and fixing volume. The color is measured in a spectrophotometer at 578nm wavelength within 1 h.

Before measuring the light absorption value of the sample, 0ml, 1ml, 3ml, 5ml, 7 ml, 9 ml, 11 ml and 13ml of nitrogen working solution are respectively taken and transferred into a 50ml volumetric flask, and distilled water is added to the volumetric flask to reach 20 ml. Then 4ml of sodium phenolate solution and 3ml of sodium hypochlorite solution are added and shaken up. Developing after 20min, and fixing volume. The color is compared on a spectrophotometer at 578nm wavelength within 1 h. And then, drawing a standard curve by taking the concentration of the nitrogen working solution as an abscissa and the light absorption value as an ordinate.

The nitrogen working solution is 0.4717g of ammonium sulfate dissolved in water and diluted to 1000ml, so as to obtain 1ml of standard solution containing 0.1mg of nitrogen; then the solution is diluted by 10 times (10 ml of standard solution is sucked to 100ml) to prepare the working solution of nitrogen.

Each sample was set up with a matrix-free control, with distilled water instead of matrix, and the other operations were identical to the sample experiments. Each experiment is provided with a soilless control, no soil sample is added, and other operations are the same as the sample experiment.

1g of NH in the soil after 24h₃The number of milligrams of-N represents the soil enzyme activity (Ure). And (4) calculating a result:

wherein: a is_{Sample (I)}-NH of the absorbance of the sample by a standard curve₃-N milligrams; mg of

a_Soil-less-NH calculated from the light absorption values of the soilless contrast by a standard curve₃-N milligrams; mg of

a_{Without matrix}NH determined by standard curve without matrix control absorbance₃-N milligrams; mg of

V is the volume of the developing solution; ml of

n-fractional times, i.e. volume of leachate/volume of filtrate taken up

m is the weight of the drying soil; g

The invention uses a statistical method to measure the plant morbidity, and the specific formula of the plant morbidity is as follows:

TABLE 2 influence of the mixing ratio of Bacillus subtilis, Candida utilis and Rhodopseudomonas palustris on the soil

The bacillus subtilis has a strong effect in soil, can generate various active substances, inhibits the breeding of germs and pests in the soil, and can improve the disease resistance and lodging resistance of plants. Candida utilis can decompose potassium and silicon in minerals and phosphorus in apatite, has the functions of dissolving phosphorus, releasing potassium and fixing nitrogen, and can produce organic acid, amino acid, polysaccharide, hormone and other matters favorable to plant absorption and utilization during growth and propagation. Rhodopseudomonas palustris has the effect of promoting the proliferation of soil microorganisms. Therefore, the bacillus subtilis, the candida utilis and the rhodopseudomonas palustris are mixed according to the mass ratio of 5-7: 1-3: 1-3 mixing.

From example 2 and table 2, it can be seen that adding the prepared fermented microbial culture to soil can effectively improve the urease activity in soil and effectively reduce the soil morbidity. The added bacillus subtilis, candida utilis and rhodopseudomonas palustris are mixed according to the mass ratio of 7: 2: 1, the soil restoration modifier prepared by fermenting the prepared fermentation mother liquor ensures that the activity of soil urease is the best, and the urease activity is positively correlated with the microbial quantity, the organic matter content, the total nitrogen and the quick-acting nitrogen content of soil. And the plant morbidity can be 0 at the lowest.

Example 3:

carrying out pure strain culture and fermentation on bacillus subtilis, candida utilis and rhodopseudomonas palustris according to respective culture medium and culture step conditions, and then mixing the materials according to a proportion of 7: 2: 1 mixing to prepare a yeast solution.

Adding organic compound fertilizer and inorganic fertilizer into the fermented microbial culture according to the mass ratio of 5: 3: 2. and (3) uniformly mixing to obtain the soil remediation modifier, and drying, disinfecting, killing insects and storing the obtained soil remediation modifier for later use. The proportions of the added organic compound fertilizer, inorganic fertilizer and microbial culture and the effect on the soil are shown in table 3.

100kg of soil restoration improver is uniformly added into one mu (666 square meters) of soil. Random sampling 1m after 4 weeks²The sample was sampled 25 times, measured and averaged, and the data obtained are shown in table 3.

The invention adopts a Kjeldahl method to measure the total nitrogen of soil, and the concrete operation is as follows:

accurately weighing 0.5g of air-dried soil sample which is sieved by a 60-mesh sieve, transferring the air-dried soil sample into a dry Kai bottle, adding 1.5g of reductive mixed catalyst (100 g of potassium sulfate, 10g of blue sulfate pentahydrate and 1g of selenium powder, uniformly mixing and grinding), adding 4ml of concentrated sulfuric acid with the concentration of 98%, and putting the mixture into a sterilizer in a fume hood to boil for 1.5h until the content becomes clear light blue. Cooling after digestion. The flask was placed under the adapter of a condenser tube, and the mouth of the tube was submerged in a 2% boric acid solution containing 2.5ml of a mixed indicator (bromocresol green 0.5g and methyl red 0.1g dissolved in 100ml of a 95% ethanol solution, and the pH was adjusted to 4.5 with NaOH or HCl), and the water flow in the condensate was turned on to conduct distillation. Distilling for 5min after the liquid turns blue, separating the lower end of the condenser pipe from the liquid level of boric acid, and flushing the outside of the pipe with distilled water. Titrate to red with 0.01 equivalents of the hydrochloric acid standard solution and record the volume of all the hydrochloric acid standard solution consumed.

The hydrochloric acid standard solution is 0.84ml of concentrated hydrochloric acid with the specific gravity of 1.19, is diluted to 1000ml by distilled water, and is calibrated by a reference substance.

A set of blanks was set and the procedure was exactly the same except that no sample was added. And (4) calculating a result:

V₂-the volume of the hydrochloric acid standard solution used when titrating the sample liquid; ml of

V₃-the volume of hydrochloric acid standard solution used for titration of the blank; ml of

D is the equivalent concentration of the hydrochloric acid standard solution.

W-soil sample weight, g

0.014-milliequivalent of Nitrogen

The invention adopts a sodium bicarbonate method to measure soil available phosphorus, and the concrete operations are as follows:

accurately weighing 2.50g of air-dried soil sample passing through a 1mm sieve in a 250ml triangular flask, and adding 50ml of 0.5M NaHCO₃(PH is 8.5), adding a spoon of phosphorus-free activated carbon, plugging a bottle stopper, oscillating for 30min at 20-25 ℃, taking out, filtering in a triangular flask by using a dry leak and phosphorus-free filter paper, and simultaneously performing a blank experiment of the reagent. Sucking 10ml of filtrate into a 50ml volumetric flask, developing with 5ml of molybdenum-antimony anti-reagent, and shaking up with distilled water to constant volume. Standing at room temperature for 30min, and performing color comparison with red filter or 660nm lightThe optical density of the measurement solution was read at a light transmittance of 100 (i.e., an optical density of 0), and the phosphorus concentration (mg/kg) of the color-developing solution was checked on the calibration curve.

0ml, 1ml, 2 ml, 3ml, 4ml, 5ml and 6 ml of standard solution containing 5mg/kg of phosphorus are respectively added into a 50ml volumetric flask, and 0.5M NaHCO is added₃Adding water into 10ml of the solution, adding 5ml of molybdenum-antimony color-resisting agent after adding 30 ml of the solution, shaking up and fixing the volume to obtain standard solutions of 0, 0.1, 0.2, 0.3, 0.4, 0.5 and 0.6mg/kg of phosphorus. And (4) carrying out color comparison with the solution to be measured at the same time, reading the absorption value, and drawing a standard curve by taking the absorption value as a vertical coordinate and taking the number of mg/kg of phosphorus on the square coordinate paper as a horizontal coordinate.

The phosphorus-free activated carbon powder is prepared by soaking activated carbon powder with 1:1HCl overnight, then performing air suction filtration by using a flat funnel, and washing with water until no HCl exists. Then 0.5M NaHCO was added₃Soaking overnight, filtering on a flat funnel with suction, washing NaHCO with water₃And finally, checking until no phosphorus exists, and drying for later use.

The molybdenum antimony color-developing resisting agent is potassium antimony tartrate KSbOC₄H₄O₆0.5g was dissolved in 100ml of water to make a 5% solution. 20g of ammonium molybdate is weighed and dissolved in 450ml of water, 208.3ml of concentrated sulfuric acid is slowly added while stirring, 100ml of 0.5 percent antimony tartrate potassium solution is added into the ammonium molybdate solution, and finally, 1 liter of the solution is added to be fully shaken and stored in a brown bottle to obtain the molybdenum-antimony mixed solution. Weighing 1.5g of L-ascorbic acid solution in 100ml of molybdenum-antimony mixed solution to be mixed uniformly on the day before use, thus obtaining the molybdenum-antimony anti-reagent.

And (4) calculating a result:

V₄-the number of phosphorus in the developing solution is found from the working curve; mg/kg

50-volume of developing solution; ml of

A-dividing and taking times is equal to the total volume (50ml) of leaching liquor/ml of leaching liquor

M, drying the soil sample; g

The invention adopts a flame photometry to measure the soil quick-acting potassium, and the specific operation is as follows:

accurately weighing 5.00g of air-dried soil passing through 1mm sieve mesh in a 100mL triangular flask, and adding 1mol L of air-dried soil^-1NH₄50mL of OAc solution was placed in a rubber stopper, shaken for 30min, and filtered through dry, regular qualitative filter paper. The filtrate was contained in a small triangular flask and measured on a flame photometer together with a potassium standard series solution. The readings on the galvanometers are recorded and the concentrations are then determined from the standard curve.

Diluting with 500mg/kg or 100mg/kg potassium standard solution to 0, 1, 3, 5, 10, 15, 20, 30, 50mg/kg potassium series solution, and adding 1mol L^-1The neutral ammonium acetate solution is diluted to a constant volume to offset the interference of ammonium acetate, and a curve is drawn by taking the concentration as a horizontal coordinate.

The 1mol L^-1The neutral ammonium acetate solution, 77.09g of chemically pure ammonium acetate, was dissolved in water to a constant volume of 1 liter, and the pH was finally adjusted to 7.0.

And (4) calculating a result:

V₅-the amount of leaching agent added; ml of

M₁-drying the soil sample; g

Finding the mg/kg number-finding the corresponding mg/kg number from the standard curve

TABLE 3 proportion of organic Compound fertilizers, inorganic fertilizers and microbial cultures and Effect on soil

From example 3 and table 3, it can be seen that when the ratio of bacillus subtilis, candida utilis and rhodopseudomonas palustris in the microbial culture is 7: 2: 1, and the mixing ratio of the organic compound fertilizer, the inorganic fertilizer and the microbial culture is 5: 3: 2, the prepared soil restoration modifier prepared by fermenting the prepared fermentation mother liquor enables the activity of soil enzymes to be the best, the lowest incidence rate of plants can be 0, and the contents of other nutrients such as total nitrogen, quick-acting phosphorus and quick-acting potassium in soil are greatly improved and can reach the standard of more than 2 grades of soil nutrients.

According to the invention, the organic fertilizer, the inorganic fertilizer and the microbial culture are fused to produce the microbial regenerative fertilizer for restoring, reducing and improving soil fertility, so as to promote rapid and mass propagation and growth of beneficial microorganisms artificially propagated. The beneficial microorganism proliferation and soil active enzyme are greatly improved, the diversity of soil microbial communities is recovered, the air permeability is enhanced, the soil hardening problem is effectively solved, and the soil micro-ecological environment is improved.

It should be noted that the above-mentioned embodiments are only for illustrating the technical solutions of the present invention and not for limiting, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions may be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, which should be covered by the claims of the present invention.

Claims

1. The preparation method of the conditioner for repairing soil is characterized by comprising the following steps: comprises the steps of (a) preparing a mixture of a plurality of raw materials,

2. The method for preparing a soil amendment for restoration according to claim 1, wherein the method comprises the following steps: the respective culture of the bacillus subtilis, the candida utilis and the rhodopseudomonas palustris comprises the steps of pure strain culture and fermentation according to respective culture medium and culture step conditions, and then the pure strain culture and the fermentation are mixed in proportion to prepare a yeast solution.

3. The method for preparing a soil amendment for restoration according to claim 2, wherein the method comprises the following steps: the respective culture media, including,

4. The method for preparing a soil amendment for restoration according to claim 2, wherein the method comprises the following steps: the culturing step comprises

5. The method for preparing a soil amendment for restoration according to claim 2, wherein the method comprises the following steps: the fermentation mother liquor prepared according to the proportion comprises 45-70% of bacillus subtilis, 15-30% of candida utilis and 10-30% of rhodopseudomonas palustris.

6. The method for preparing a soil amendment for restoration according to claim 1, wherein the method comprises the following steps: the fermentation substrate comprises 15% of corn, 30% of soybean meal and 65% of bran.

7. The method for preparing a soil amendment for restoration according to claim 1, wherein the method comprises the following steps: the anaerobic fermentation comprises the following steps of,

8. The method for preparing a soil amendment for restoration according to claim 1, wherein the method comprises the following steps: the organic compound fertilizer comprises one or more of wet compost, mud fertilizer, stable manure and the like.

9. The method for preparing a soil amendment for restoration according to claim 1, wherein the method comprises the following steps: the inorganic fertilizer comprises 50-70 g/kg of nitrogenous fertilizer, 40-60 g/kg of phosphate fertilizer and 60-70 g/kg of potash fertilizer.

10. The method for preparing a soil amendment for restoration according to claim 1, wherein the method comprises the following steps: the organic fertilizer and the inorganic fertilizer are mixed according to the proportion, wherein the mass ratio of the organic compound fertilizer to the inorganic fertilizer to the microbial culture is 5: 3: 2.