CN101781569A - Soil improvement and anti continuous cropping multifunctional biological agent - Google Patents
Soil improvement and anti continuous cropping multifunctional biological agent Download PDFInfo
- Publication number
- CN101781569A CN101781569A CN200910230985A CN200910230985A CN101781569A CN 101781569 A CN101781569 A CN 101781569A CN 200910230985 A CN200910230985 A CN 200910230985A CN 200910230985 A CN200910230985 A CN 200910230985A CN 101781569 A CN101781569 A CN 101781569A
- Authority
- CN
- China
- Prior art keywords
- soil
- bacillus subtilis
- liquid
- acid
- trichoderma pseudokiningii
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
- Fertilizers (AREA)
Abstract
The invention provides a soil improvement and anti continuous cropping multifunctional biological agent which contains the mixed microorganism of trichoderma pseudokoningii and bacillus subtilis, faveolate humic acid and zinc sulphate; the trichoderma pseudokoningii is a bacterial strain obtained by separating and screening maize straw; the bacterial strain is named after (Trichoderma pseudokoningii) DLDI and is preserved in China general microbiological culture collection (CGMCC), and the number thereof is CGMCC NO.3221; the bacillus subtilis obtained by separating soil is named after (bacillus subtilis) DLD2 and is preserved in China general microbiological culture collection (CGMCC), and the number is CGMCCNO.3222; the biological agent has the effects that (1) biocontrol microorganism is used for blocking harmful flora from infecting plants and killing pathogenic bacteria, induces the plants to generate disease resistance and prevents dead seedlings from rotting roots; (2) the microorganism and the faveolate humic acid are compounded to induce the root system to grow, thus realizing root regeneration and plant regeneration and inhibiting the anti continuous cropping diseases; and (3) the faveolate humic acid and the zinc sulphate are used for breaking the soil hardening of the soil, forming the crumble structure, realizing no deep ploughing, retaining water, alleviating fertilizer damage and increasing the soil productivity.
Description
Technical field
The present invention relates to soil improvement agent and soil successive planting resistant agent field, particularly a kind of soil improvement and successive planting resistant agent two are imitated multifunctional biological agent of unification and preparation method thereof.
Background technology
Good edaphic condition is the prerequisite of crop high yield, but because the weather arid, chemical fertilizer excessively uses, irrational cultivation and management, and reasons such as continuous cropping cause that soil ecology worsens, crop failure has caused enormous economic loss.The deterioration of soil ecology mainly is root system water, vapour, the fertile unbalance and microbial continuous cropping disease of continuous cropping cause of disease that soil compaction causes to the influence of crop.The method of exploring is effectively broken soil compaction, and the control continuous cropping disease has huge economic benefit and social effect.
At present, the method for breaking soil compaction mainly is the machinery deep ploughing and uses soil improvement agent.The machinery deep ploughing is to utilize high-power agricultural machine, and plowed soils thickness reaches more than the 60cm, loose hardened soil, and the reconstruct crumb structure is increase soil fertility, water-retaining effect, this method is pollution-free, does not destroy profitable strain.Shortcoming is that the soil amount of stirring is big, forms uneven ridges easily, influences the land smoothing degree, and labour intensity is big, the cost height.Use effectively improved soil nodule structure of soil improvement agent, play the effect of ventilation, water-retaining effect.Soil improvement agent comprises synthetic modifying agent and natural modifying agent.Polyacrylamide, synthetic modifying agent cost height such as asphalt emulsion, the time length is short, and ecotope is had disadvantageous effect.Natural modifying agent such as saccharan, humic acid, peat have same improved effect, but cost is low relatively, and the greenization environmental protection has remarkable advantages.
Anti-continuous cropping disease main method has: pyroprocessing, and chemical agent is handled, and microbiobacterial agent is handled.Pyroprocessing is to improve the soil moisture (40-50 ℃, 15 days) by modes such as covering with plastic film to reach the effect of killing pathogenic bacteria.This method can only be carried out the shallow-layer sterilization to the face of land, and deep soil is not had unusual effect.The method that chemical agent is handled, more with trichloronitromethane and monobromethane use, advantage is that effect is remarkable to the strong toxicity of pathogenic soil bacterium.Shortcoming is that environment is caused disadvantageous effect, when killing pathogenic bacteria, the profitable strain in the soil is destroyed, and is unfavorable to plant growth, to the toxic effect of people and animals.Microbial bactericide utilizes the competition between microorganism by executing probioticses such as biocontrol microorganisms, fixed nitrogen, phosphorus decomposing, potassium decomposing outward, antagonism, and effects such as superparasitism suppress or kill pathogenic bacteria, and protection also promotes plant root growth, increases soil fertility.It is a kind of method of anti-continuous cropping disease of high-efficiency environment friendly.
Harden and the continuous cropping disease of soil is accompanied often, but also do not have a kind of effective means at present, possesses the effect of soil improvement and continuous cropping disease control simultaneously.
Summary of the invention
The object of the invention is to provide a kind of reclamation result of natural soil conditioner and multifunctional biological agent of microbe soil bactericidal agent for preventing and treating continuous cropping disease effect of combining.Its effect is that (1) utilizes biological and ecological methods to prevent plant disease, pests, and erosion microorganism blocking-up pernicious bacteria to infect plant and kill pathogenic bacteria, and inducing plant produces disease resistance, mashed of anti-dead seedling; (2) utilize the composite synergistic function of microorganism and xanthohumic acid, induce root growth, realize strong plantlets and rootage and anti-continuous cropping disease; (3) utilize xanthohumic acid, zinc sulfate, break soil compaction, form crumb structure, realize exempting from deep ploughing and do, water-retaining effect, alleviate fertilizer damage, increase fertility of land.
The component of biotechnological formulation of the present invention comprises: complex microorganism and soil improvement agent xanthohumic acid and the zinc sulfate etc. of trichoderma pseudokiningii (Trichoderma pseudokiningii) and subtilis (Bacillus subtilis), composite soil improvement, the anti continuous cropping multifunctional biological agent made.
Described trichoderma pseudokiningii bacterial strain is to separate from Jinan City's Licheng District corn stalk, the eugonic bacterial strain that utilizes the PDA culture plate to cultivate, screen, called after trichoderma pseudokiningii (Trichoderma pseudokiningii) DLD1, in on August 12nd, 2009 in China Committee for Culture Collection of Microorganisms common micro-organisms center (CGMCC) preservation, be numbered CGMCCNO.3221, the address is the Datun Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica.
Trichoderma pseudokiningii (Trichoderma pseudokiningii) DLD1 has following feature:
This microbial strains can be in 25 ℃ of-30 ℃ of scopes growth fast, optimum growth temperature is 28 ℃, suppresses growth in being higher than 35 ℃ environment; In the PDA culture plate, white mycelium, sporophore main shaft secondary branch complex structure has 2-3 and goes back to branch; Bottle metulae portion shrinks obviously, and expand the centre, and the top attenuates; ultimate bottle stalk prolongs, and base portion does not attenuate, and the conidiogenous cell size is the μ m of (3.00-7.50) μ m * (1.25-2.00); conidium is short cylindrical shape, and size is the μ m of (2.5-4.5) μ m * (2.0-2.5).
Described bacillus subtilis strain is to separate from the vegetable soil of Shouguang, Shandong, use extractum carnis, protein culture medium, utilize spread plate method cultivation screening to obtain, called after subtilis (Bacillus subtilis) DLD2, this bacterial classification on August 12nd, 2009 in China Committee for Culture Collection of Microorganisms common micro-organisms center (CGMCC) preservation, be numbered CGMCC NO.3222, the address is the Datun Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica.
Subtilis (Bacillus subtilis) DLD2 has following feature:
0.7～0.8 * 2～3 microns of individual cells, uniform coloring.No pod membrane, peritrichous can move.Gram-positive microorganism, 0.6～0.9 * 1.0～1.5 microns of gemma, oval to column, be positioned at thalline central authorities or inclined to one side slightly, gemma forms the back thalline and does not expand.The bacterium colony surface irregularity is opaque, dirty white or little yellow, and when growing in the liquid medium within, the normal wrinkle mould that forms.Optimum growth temperature is 28 ℃.
The complex microorganism of described trichoderma pseudokiningii (Trichoderma pseudokiningii) and subtilis (Bacillus subtilis) is the compound microorganism ferments liquid liquid that mixes with trichoderma pseudokiningii fermented liquid and fermentation of bacillus subtilis liquid.
Trichoderma pseudokiningii preparation of fermentation liquid method is as follows:
At first trichoderma pseudokiningii (Trichoderma pseudokiningii) DLD1 bacterial strain is utilized the dull and stereotyped activation of PDA, receive in the liquid PDA substratum, cultivate on shaking table the sterilization back, makes seed liquor; Be fermention medium with potato, bran mass again, successively in seeding tank, secondary seed jar and fermentor tank, stir aerobic fermentation; Mixing, embedding, concentrated make the trichoderma pseudokiningii fermented liquid then.
Concrete preparation method is:
The trichoderma pseudokiningii seed culture:
At first the trichoderma pseudokiningii bacterial strain is utilized the dull and stereotyped activation of PDA 24h, after receive in liquid PDA substratum (potato 200g/L, the sucrose 100g/L) triangular flask, cultivate on shaking table the sterilization back, culture condition is 28 ℃, 100rpm, cultivates 36h, obtains seed liquor.
The trichoderma pseudokiningii fermentation:
Fermention medium is potato, bran mass, and composition is as follows: wheat bran 40g/L, potato 50g/L, sucrose 20g/L, KH
2PO
40.5g/L, MgSO
47H
2O 0.5g/L, CaCl
20.25g/L.
The condition of fermentation culture: pH5-7,28 ℃ of culture temperature, rotating speed 20-50rpm, inoculum size 3%-7%.
Fermentation process: by inoculum size bacterial classification is received in the seeding tank, stirred aerated culture 36h, enter the secondary seed jar then and stir the cultivation 36h that ventilates, stir aerated culture 96h in the fermentor tank that reinjects; Enter the high-speed shearing machine mixing then, embedding enters the economic benefits and social benefits thickener again, and under 45 ℃ temperature, dense system trichoderma pseudokiningii culture makes the trichoderma pseudokiningii fermented liquid.Contain Trichoderma silk dry weight 0.1-1.0g in every 100ml trichoderma pseudokiningii fermented liquid of gained, the fermentation culture effect is to contain Trichoderma silk dry weight 0.9-1.0g in every 100ml trichoderma pseudokiningii fermented liquid preferably
The preparation method of fermentation of bacillus subtilis liquid is as follows:
Subtilis (Bacillus subtilis) DLD2 bacterial classification through the dull and stereotyped activation of LB, is received in the liquid LB substratum, carried out shaking table and cultivate; Inoculate and enter seeding tank, secondary seed jar, fermentor tank, stir aerobic fermentation; Enter emulsification in the emulsion tank, embedding then, enter the economic benefits and social benefits thickener again and concentrate and to make the subtilis culture, be fermentation of bacillus subtilis liquid.Bacterial content reaches 1 * 10 in the bacterium liquid
9-3 * 10
10/ ml, ideal bacterial content are 3,000,000,000/ml.
Concrete preparation method is:
The subtilis seed culture:
The fermentation of subtilis: Bacillus subtilis strain through the dull and stereotyped activation of LB, is received in the liquid LB substratum (Tryptones 10g/L, yeast extract 5g/L, NaCl 10g/L) again, and under 28 ℃, rotating speed 120rpm, shaking table is cultivated 24h.
Fermention medium is: sucrose 20g/L, ammonium sulfate 5g/L, wheat bran 50g/L, trisodium citrate 2.5g/L, K
2HPO
43H
2O0.3g/L, MgSO47H
2O 0.5g/L, FeSO
47H
2O 0.05g/L.
The condition of fermentation culture is as follows: 30 ℃ of leavening temperatures, initial pH value 7.2, inoculum size 3%-7%, the suitableeest inoculum size 5%.
Fermentation process: send into stirring aerated culture 24h in the seeding tank by inoculum size, enter again and stir aerated culture 24h in the secondary seed jar, inject fermentor tank then and stir aerated culture 48h; Fermented liquid enters emulsification in the emulsion tank, embedding, enters the economic benefits and social benefits thickener again, concentrates the system subtilis under 45 ℃ temperature, makes fermentation of bacillus subtilis liquid.Bacterial content reaches 1 * 10 in the bacterium liquid
9-3 * 10
10/ ml.
The component of biotechnological formulation of the present invention comprises: the complex microorganism of trichoderma pseudokiningii fermented liquid and fermentation of bacillus subtilis liquid and soil improvement agent xanthohumic acid and zinc sulfate, and its composition weight umber is as follows:
In order to improve effect and to improve the preparation performance, also can add following auxiliary component in soil improvement of the present invention, the anti continuous cropping multifunctional biological agent: vitamins C and/or α naa and/or chlorogenic acid and/or aminosallcylic acid and dextrin etc.
Concrete feed composition and the parts by weight of preparing soil improvement of the present invention, anti continuous cropping multifunctional biological agent are as follows:
Wherein:
Trichoderma pseudokiningii fermented liquid: contain Trichoderma silk dry weight 0.9-1.0g in the 100ml fermented liquid;
Fermentation of bacillus liquid: genus bacillus 3,000,000,000 in every milliliter of fermentation of bacillus liquid;
Other component concentrations are by 100%.
The preparation method of soil improvement of the present invention, anti continuous cropping multifunctional biological agent is:
1. prepare the koning trichoderma fermented liquid.
2. prepare fermentation of bacillus subtilis liquid.
3. it is standby above-mentioned two kinds of fermented liquids mixing to be obtained complex microorganism composition A.
4. with xanthohumic acid, zinc sulfate adds in 50-60 ℃ the water fully stirring and dissolving, and chelating progressively is cooled to 30 ± 2 ℃; B is standby as the soil improvement composition.
5. aminosallcylic acid, chlorogenic acid, α naa, vitamins C add in 50-60 ℃ the water fully stirring and dissolving, progressively are cooled to 30 ± 2 ℃, and C is standby as the plant inducible factor.
6. last with above-mentioned standby complex microorganism composition A, soil improvement composition B, plant inducible factor composition C merging in material-compound tank, mixing, interpolation dextrin composition also carry out emulsification, and be qualified after testing again, is finished product of the present invention.
Beneficial effect
Trichoderma pseudokiningii of the present invention (Trichoderma pseudokiningii) DLD1 is a kind of plant biological control strain with multiple disease-resistant mechanism, to the person poultry harmless, does not produce environmental pollution, and growing ability is extremely strong.This bacterium can breed fast at crop root, forms the protection structure, the blocking-up pathogen infection.Simultaneously can pass through hyperparasitism, secrete various bacteriocidal substances and cell wall lysis enzyme, play the effect of killing pathogenic bacteria.In addition, trichoderma pseudokiningii can be induced and be made the deposits yields disease resistance and promote plant-growth.Realize the crop strong plantlets and rootage, effectively reduced mashed of dead seedling.
Subtilis of the present invention (Bacillus subtilis) DLD2 is the biological and ecological methods to prevent plant disease, pests, and erosion microorganism to the person poultry harmless that extensively exists in a kind of physical environment.Subtilis can reduce infecting of bacterial pathogen by the competition effect of ecological niche, reduces the fungal pathogen bacterium by bacteriolysis and infects.In addition, can also secrete antimicrobial substance and the cell wall lysis enzyme is killed pathogenic bacteria.Subtilis is a class plant growth-promoting root circle bacterium (Plant growth-promoting rhizobaceria), can promote plant growth.
The mixing microorganisms of trichoderma pseudokiningii of the present invention and subtilis has enlarged the disease-resistant spectrum of said preparation, can effectively resist 129 kinds of pathogenic fungies and bacterium.Under the mutual synergism of two kinds of microorganisms, said preparation can produce multiple soil improvement composition and plant disease-resistant inducible factor in soil.Both mixed effects are better than single effect.
Xanthohumic acid of the present invention belongs to humic-acid kind, can promote soil granular to form, and makes loosing soil, has good adsorption.Thereby protect fertile, steady fertilizer, water conservation, ventilative, overcome the phenomenon that hardens of soil, lay the first stone for increasing soil fertility.Humic acids can also promote root growth and development to realize strong sprout by promoting the crop respiration.Humic acids is a kind of natural soil conditioner of high-efficiency environment friendly, and manufacturing process is simple, and is cheap.
Zinc sulfate of the present invention is a kind of trace element supplement.Because the plantation of the continuous cropping of crop, constantly absorb the specific trace element in the soil and can not get replenishing, especially zinc element is the easiest lacks in the continuous cropping continuous cropping soil, and additional zinc sulfate helps to alleviate the underproduction that continuous cropping causes.
Aminosallcylic acid, chlorogenic acid, the α naa, vitamins C helps promoting plant growth, disease resistance enhancemen as plant inducible factor C.
Above-mentioned each component rationally composite can produce synergistic function.
The present invention has soil improvement and anti-continuous cropping disease double effects, has solved the soil ecology deterioration more comprehensively and has caused soil compaction and continuous cropping disease problem.
Embodiment:
Example 1, soil improvement of the present invention, anti continuous cropping multifunctional biological agent are formed by the preparation of raw material of following (part) by weight ratio:
Trichoderma pseudokiningii fermented liquid 18, fermentation of bacillus liquid 22, xanthohumic acid 15, zinc sulfate 4, vitamins C 7, α naa 0.8, chlorogenic acid 10, aminosallcylic acid 7, dextrin 8, add water to 100 parts.
Example 2, soil improvement of the present invention, anti continuous cropping multifunctional biological agent are formed by the preparation of raw material of following (part) by weight ratio:
Trichoderma pseudokiningii fermented liquid 15, fermentation of bacillus liquid 20, xanthohumic acid 10, zinc sulfate 3, vitamins C 6, α naa 0.7, chlorogenic acid 9, aminosallcylic acid 6, dextrin 7, add water to 100 parts.
Example 3, soil improvement of the present invention, anti continuous cropping multifunctional biological agent are formed by the preparation of raw material of following (part) by weight ratio: trichoderma pseudokiningii fermented liquid 20, fermentation of bacillus liquid 25, xanthohumic acid 20, zinc sulfate 5, vitamins C 7, α naa 0.8, chlorogenic acid 9, aminosallcylic acid 6, dextrin 8, add water to 100 parts.
The preparation method of above-mentioned soil improvement, anti continuous cropping multifunctional biological agent is:
1. the preparation method of trichoderma pseudokiningii fermentation: answer aseptic technique in the processing; At first the trichoderma pseudokiningii bacterial strain is utilized the dull and stereotyped activation of PDA 24h, after receive in the liquid PDA triangular flask, 28 ℃, 100r/min cultivates 36h and obtains seed liquor, receives in the seeding tank by 3% inoculum size, stir aerated culture 36h, enter secondary seed jar stir culture 36h again, inject fermentor tank then and stir aerated culture 96h, enter the high-speed shearing machine mixing again, embedding enters the wooden mould culture of economic benefits and social benefits thickener dense system under 45 ℃ temperature again; Substratum is potato, bran mass (wheat bran 40g/L, potato 50g/L, sucrose 20g/L, KH in fermentation culture
2PO
40.5g/L, MgSO
47H
2O 0.5g/L, CaCl
20.25g/L, pH5-7,28 ℃ of culture temperature, rotating speed 20-50rpm, inoculum size 3%-7%).
2. the preparation method of fermentation of bacillus subtilis: answer aseptic technique in the processing; Bacillus subtilis strain is activated through the NA flat board, receive liquid NA substratum and on shaking table, cultivate 24h, carry out stirring in the seeding tank aerated culture 24h by 3% inoculum size, in entering the secondary seed jar, stir aerated culture 24h, injecting fermentor tank then stirs and to cultivate 48h the same period, enter emulsification embedding in the emulsion tank again, entering the economic benefits and social benefits thickener concentrates system under 45 ℃ temperature subtilis culture;
3. it is standby above-mentioned two kinds of fermenting cultures mixing to be obtained microbe composition A.
4. with xanthohumic acid, zinc sulfate adds in 50-60 ℃ the water fully stirring and dissolving, and chelating progressively is cooled to 30 ± 2 ℃; B is standby as the soil improvement composition.
5. aminosallcylic acid, chlorogenic acid, α naa, vitamins C add in 50-60 ℃ the water fully stirring and dissolving, progressively are cooled to 30 ± 2 ℃, and C is standby as the plant inducible factor.
6. last with above-mentioned standby withered microbe composition A, soil improvement composition B, plant inducible factor composition C merging in material-compound tank, mixing, interpolation dextrin composition also carry out emulsification, qualifiedly after testing again are finished product of the present invention;
Concrete using method can be irritated root, be watered, sprays, dips in root with water.
Concrete application example and effect:
Example 1:
(1) the proportioning raw materials reference example 1 of soil improvement activator;
(2) using method: the strawberry booth carries out in Qufu City Dong local, Shandong.Use the present invention to dilute 60 times and spray ground, underground in conjunction with plough under wholely.Use the present invention to dilute 300-400 after the strawberry field planting and doubly spray ground, logotype 3-4 time sprayed 10-15 days at interval.Every 666.7m
2The each 2-3 liter of consumption.Strawberry cultivars is beautiful king No. 1.
In adjacent plot not use the present invention in contrast.Every sub-district area is 100m
2, three repetitions.Other management are all with conventional.
Upgrowth situation to the root of seedling phase, stem, leaf is investigated.
Test-results sees Table 1:
Table 1 the present invention is to the influence of strawberry seedling phase root, stem, leaf
As shown in Table 1, use the present invention after, average individual plant root system number, root length, capillary radical amount have increased by 55.6%, 22.7% and 73.0% respectively, average stem rugosity has increased by 1.25 times, blade quantity, the speed of growth, effectively leaf age has improved 83.3%, 22.2% and 46.7% respectively.Illustrate and use the present invention can improve edatope, promote the strawberry strong plantlets and rootage, the effect highly significant.
Example 2
(1) the proportioning raw materials reference example 1 of soil improvement activator.
(2) using method: carry out in Jinxiang County, Shandong, this regional cotton continuous cropping was planted in more than 5 years, and the soil compaction that the unreasonable use of chemical fertilizer causes is serious, and soil-borne disease is in vogue.Trial crops is a cotton.Use the present invention to dilute 60 times and spray ground and fertilizer and mix and execute, underground in conjunction with plough under wholely.Every 666.7m
2Consumption 2-3 liter.
In adjacent plot not use the present invention in contrast.Every sub-district area is 100m
2, three repetitions.Other management are all with conventional.
Carry out the investigation of sprout term disease prevention effect, be respectively after the field planting 10 days, 20 days, 30 days, 40 days, 45 days, to this stage by sprout term diseases such as the samping off that causes because of soil-borne disease, cold spell in later spring, temperature, ground temperature, high humidity, damping-off, root rot, red rot, carry out investigation statistics.
Test-results sees Table 2:
Table 2 the present invention is to the influence of cotton in seedling stage resistance against diseases
As shown in Table 2, used the present invention after, the seedling of dying of illness, the seedling of catching an illness has obtained effectively control in whole seedling stage (10 days-45 days), has kept higher healthy seedling rate, shows that this invention can be in cotton easily susceptible seedling stage, improve edatope, effectively control the generation of soil-borne disease.The continuous cropping disease that causes for continuous cropping for many years has significant preventive effect.
Example 3
The prescription reference formulation embodiment 1 of (1) soil improvement, anti continuous cropping multifunctional biological agent.
(2) using method: carry out the soil improvement test in Shandong university student's thing chemistry and molecular biology research institute experimental plot.With experimental plot soil as experimental subjects.Using the present invention to dilute 60 times and spray soil, is control group to spray distilled water.Sampling, measuring method are with reference to State Standard of the People's Republic of China's soil environment quality standard (GB15618-1995).Measure control group and soil organic matter content, water holding capacity, the unit weight of using experimental group of the present invention respectively.
Table 3 the present invention is to the influence of the soil organism, water content, unit weight
As shown in Table 3, use the present invention after, the soil organism, water holding capacity, the soil weight all are significantly increased.The result shows that the present invention can effectively improve the soil particle degree, breaks soil compaction, increases soil fertility and water holding capacity.Has tangible reclamation result.
Example 4
The prescription reference formulation embodiment 1 of (1) soil improvement, anti continuous cropping multifunctional biological agent.
(2) using method: carry out soil parameters in Shandong university student's thing chemistry and molecular biology research institute experimental plot and detect.Soil adopts with experimental plot soil.Use the present invention to dilute 60 times and spray soil, use distilled water in contrast with potted plant cucumber seedling.Totally 100 basins, every basin 3 strains.Other management are all with conventional.
Using back 0d respectively, 3d, 6d, 9d, 12d, 24d takes a sample to seedlings root.Method is with reference to Li Chunxi chief editor " biostatistics " the 4th edition (Science Press).Each sampling spot triplicate.Measure plant disease-resistant relevant enzyme vigor, polyphenol oxides enzyme (PPO), superoxide-dismutase (SOD), peroxidase (POD), beta-1,3-glucanase content (β-1,3-Gluconase).Method is with reference to Zhang Zhiliang chief editor " plant physiology test direction " third edition (Higher Education Publishing House).
Table 4 the present invention is to the influence (unit: U/mg) of disease-resistant relevant enzyme vigor in the root system
As shown in Table 4, use the present invention after, the vigor of several disease-resistant key enzymes in the root system of plant improved rapidly in 3 days, these enzymes can effectively suppress, kill the soil-borne disease that infects root system in the soil.Enzyme activity all is significantly higher than control group in after using 24 days.Prove that in the Physiology and biochemistry level this product can effectively resist soil-borne disease of crop.
Claims (9)
1. a soil improvement, anti continuous cropping multifunctional biological agent is characterized in that the component of described biotechnological formulation comprises trichoderma pseudokiningii (Trichoderma pseudokiningii) and subtilis (Bacillussubtilis) complex microorganism and soil improvement agent xanthohumic acid and zinc sulfate.
2. biotechnological formulation as claimed in claim 1, it is characterized in that said trichoderma pseudokiningii bacterial strain is trichoderma pseudokiningii (Trichoderma pseudokiningii) DLD1, this bacterial strain in China Committee for Culture Collection of Microorganisms common micro-organisms center (CGMCC) preservation, is numbered CGMCC NO.3221.
3. biotechnological formulation as claimed in claim 1, it is characterized in that said bacillus subtilis strain is subtilis (Bacillus subtilis) DLD2, this bacterial strain in China Committee for Culture Collection of Microorganisms common micro-organisms center (CGMCC) preservation, is numbered CGMCC NO.3222.
4. biotechnological formulation as claimed in claim 1 is characterized in that the parts by weight of each component of biotechnological formulation are as follows:
Trichoderma pseudokiningii fermented liquid 10-50
Fermentation of bacillus subtilis liquid 10-50
Xanthohumic acid 2-30
Zinc sulfate 0.5-5.
5. biotechnological formulation as claimed in claim 1 is characterized in that the component of biotechnological formulation also comprises vitamins C and/or α naa and/or chlorogenic acid and/or aminosallcylic acid and dextrin.
6. as claim 1 or 5 described biotechnological formulations, it is characterized in that the parts by weight of each component of biotechnological formulation are as follows:
The composition weight umber
Trichoderma pseudokiningii fermented liquid 10-50
Fermentation of bacillus subtilis liquid 10-50
Xanthohumic acid 2-30
Zinc sulfate 0.5-5
Vitamins C 4-12
α naa 0.5-1.5
Chlorogenic acid 6-15
Aminosallcylic acid 3-10 5-8 6-7
Dextrin 5-10 6-9 7-8
7. as claim 1 or 5 described biotechnological formulations, it is characterized in that the parts by weight of each component of biotechnological formulation are as follows:
The composition weight umber
Trichoderma pseudokiningii fermented liquid 10-20
Fermentation of bacillus subtilis liquid 15-30
Xanthohumic acid 5-25
Zinc sulfate 1-5
Vitamins C 5-10
α naa 0.6-1. 2
Chlorogenic acid 8-13
Aminosallcylic acid 5-8
Dextrin 6-9
8. as claim 1 or 5 described biotechnological formulations, it is characterized in that the parts by weight of each component of biotechnological formulation are as follows:
The composition weight umber
Trichoderma pseudokiningii fermented liquid 15-20
Fermentation of bacillus subtilis liquid 20-25
Xanthohumic acid 10-20
Zinc sulfate 3-5
Vitamins C 6-8
α naa 0.7-0.8
Chlorogenic acid 9-10
Aminosallcylic acid 6-7
Dextrin 7-8
9. as claim 1 or 5 described biotechnological formulations, it is characterized in that the preparation method of described biotechnological formulation is as follows:
1. prepare the trichoderma pseudokiningii fermented liquid: trichoderma pseudokiningii (Trichoderma pseudokiningii) DLD1 bacterial strain is utilized the dull and stereotyped activation of PDA, receive in the liquid PDA substratum, cultivate on shaking table the sterilization back, makes seed liquor; Successively in seeding tank, secondary seed jar and fermentor tank, stir aerobic fermentation; Mixing, embedding, concentrated make the trichoderma pseudokiningii fermented liquid then;
2. prepare fermentation of bacillus subtilis liquid: subtilis (Bacillus subtilis) DLD2 bacterial strain through the dull and stereotyped activation of LB, is received in the liquid LB substratum, carried out shaking table after the sterilization and cultivate, make seed liquor; Inoculation successively enters in seeding tank, secondary seed jar, the fermentor tank and stirs aerated culture; Enter emulsification in the emulsion tank, embedding then, after concentrating, make fermentation of bacillus subtilis liquid;
3. it is standby above-mentioned two kinds of fermented liquids mixing to be obtained microbe composition A.
4. with xanthohumic acid, zinc sulfate adds in 50-60 ℃ the water fully stirring and dissolving, and chelating progressively is cooled to 30 ± 2 ℃; B is standby as the soil improvement composition.
5. aminosallcylic acid, chlorogenic acid, α naa, vitamins C add in 50-60 ℃ the water fully stirring and dissolving, progressively are cooled to 30 ± 2 ℃, and C is standby as the plant inducible factor.
6. last with above-mentioned standby microbe composition A, soil improvement composition B, plant inducible factor composition C merging in material-compound tank, mixing, interpolation dextrin composition also carry out emulsification, and be qualified after testing again, is finished product of the present invention.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009102309856A CN101781569B (en) | 2009-11-27 | 2009-11-27 | Soil improvement and anti continuous cropping multifunctional biological agent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009102309856A CN101781569B (en) | 2009-11-27 | 2009-11-27 | Soil improvement and anti continuous cropping multifunctional biological agent |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101781569A true CN101781569A (en) | 2010-07-21 |
| CN101781569B CN101781569B (en) | 2012-11-14 |
Family
ID=42521724
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009102309856A Expired - Fee Related CN101781569B (en) | 2009-11-27 | 2009-11-27 | Soil improvement and anti continuous cropping multifunctional biological agent |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101781569B (en) |
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102030594A (en) * | 2010-11-29 | 2011-04-27 | 北京林业大学 | Silicon-calcium-iron-potassium biological magnetic fertilizer |
| CN102391876A (en) * | 2011-09-29 | 2012-03-28 | 山东靠山生物科技有限公司 | Composite biological soil modifier and application thereof |
| CN102428966A (en) * | 2011-09-29 | 2012-05-02 | 山东靠山生物科技有限公司 | Composite bio-formulation for preventing crop diseases and application thereof |
| CN102839126A (en) * | 2012-09-21 | 2012-12-26 | 宋彦耕 | Microbial agent and application of antigravity and cropping effects of microbial agent |
| CN102911670A (en) * | 2012-11-12 | 2013-02-06 | 山东大学 | Soil conditioner as well as preparation method and application of soil conditioner |
| CN102987059A (en) * | 2012-12-12 | 2013-03-27 | 吴建敏 | Breeding environment ecological regulating agent |
| CN103409357A (en) * | 2012-09-21 | 2013-11-27 | 宋彦耕 | Microbial agent and application for treating cultivating wastes |
| CN104447127A (en) * | 2014-12-17 | 2015-03-25 | 成都新朝阳作物科学有限公司 | Soil conditioner for resisting pseudo-ginseng continuous cropping diseases as well as preparation and application thereof |
| CN104557249A (en) * | 2014-11-17 | 2015-04-29 | 孟庆伟 | Microbial fertilizer |
| CN104744166A (en) * | 2015-03-26 | 2015-07-01 | 王振学 | Traditional Chinese medicine type soil microbial continuous cropping agent and preparation method thereof |
| CN106417386A (en) * | 2016-09-22 | 2017-02-22 | 黑龙江田力保生物科技有限公司 | Leaf type wettable biological agent and use method thereof |
| CN108076985A (en) * | 2017-12-14 | 2018-05-29 | 河南中威高科技化工有限公司 | Strawberry is colonized method for transplanting |
| CN108329918A (en) * | 2018-02-07 | 2018-07-27 | 浦江县美泽生物科技有限公司 | A kind of preparation method of the activator of prevention soil hardening |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1059885C (en) * | 1997-09-11 | 2000-12-27 | 赵怀久 | Soil improvement all-elements micro-fertilizer |
| CN1422828A (en) * | 2002-12-20 | 2003-06-11 | 黎保文 | Microbial compound liquid fertilizer and method for preparing the same |
| CN101126026B (en) * | 2007-10-10 | 2011-01-05 | 中国农业科学院蔬菜花卉研究所 | Vegetable field soil improving agent and its preparation method |
-
2009
- 2009-11-27 CN CN2009102309856A patent/CN101781569B/en not_active Expired - Fee Related
Cited By (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102030594A (en) * | 2010-11-29 | 2011-04-27 | 北京林业大学 | Silicon-calcium-iron-potassium biological magnetic fertilizer |
| CN102030594B (en) * | 2010-11-29 | 2013-01-16 | 北京林业大学 | Silicon-calcium-iron-potassium biological magnetic fertilizer |
| CN102391876A (en) * | 2011-09-29 | 2012-03-28 | 山东靠山生物科技有限公司 | Composite biological soil modifier and application thereof |
| CN102428966A (en) * | 2011-09-29 | 2012-05-02 | 山东靠山生物科技有限公司 | Composite bio-formulation for preventing crop diseases and application thereof |
| CN102391876B (en) * | 2011-09-29 | 2015-03-04 | 山东靠山生物科技有限公司 | Composite biological soil modifier and application thereof |
| CN102428966B (en) * | 2011-09-29 | 2014-10-22 | 耿小青 | Composite bio-formulation for preventing crop diseases and application thereof |
| CN103409357A (en) * | 2012-09-21 | 2013-11-27 | 宋彦耕 | Microbial agent and application for treating cultivating wastes |
| CN102839126B (en) * | 2012-09-21 | 2013-10-09 | 宋彦耕 | Microbial agent and application of antigravity and cropping effects of microbial agent |
| CN102839126A (en) * | 2012-09-21 | 2012-12-26 | 宋彦耕 | Microbial agent and application of antigravity and cropping effects of microbial agent |
| CN103409357B (en) * | 2012-09-21 | 2015-05-13 | 宋彦耕 | Microbial agent and application for treating cultivating wastes |
| CN102911670A (en) * | 2012-11-12 | 2013-02-06 | 山东大学 | Soil conditioner as well as preparation method and application of soil conditioner |
| CN102911670B (en) * | 2012-11-12 | 2015-03-11 | 山东大学 | Soil conditioner as well as preparation method and application of soil conditioner |
| CN102987059A (en) * | 2012-12-12 | 2013-03-27 | 吴建敏 | Breeding environment ecological regulating agent |
| CN104557249A (en) * | 2014-11-17 | 2015-04-29 | 孟庆伟 | Microbial fertilizer |
| CN104447127A (en) * | 2014-12-17 | 2015-03-25 | 成都新朝阳作物科学有限公司 | Soil conditioner for resisting pseudo-ginseng continuous cropping diseases as well as preparation and application thereof |
| CN104744166A (en) * | 2015-03-26 | 2015-07-01 | 王振学 | Traditional Chinese medicine type soil microbial continuous cropping agent and preparation method thereof |
| CN106417386A (en) * | 2016-09-22 | 2017-02-22 | 黑龙江田力保生物科技有限公司 | Leaf type wettable biological agent and use method thereof |
| CN108076985A (en) * | 2017-12-14 | 2018-05-29 | 河南中威高科技化工有限公司 | Strawberry is colonized method for transplanting |
| CN108329918A (en) * | 2018-02-07 | 2018-07-27 | 浦江县美泽生物科技有限公司 | A kind of preparation method of the activator of prevention soil hardening |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101781569B (en) | 2012-11-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101781569B (en) | Soil improvement and anti continuous cropping multifunctional biological agent | |
| CN101792669B (en) | Multi-functional biological agent containing bacillus subtilis | |
| CN102199049B (en) | Microbial organic manure and preparation method thereof | |
| CN104177137B (en) | A kind of microbial straw composing agent and preparation method thereof | |
| CN104672028B (en) | A kind of biological organic fertilizer with pest-resistant function and preparation method thereof | |
| CN103113167B (en) | Compound microbial organic fertilizer and preparation method thereof | |
| CN102337237B (en) | Functional organic material decomposing agent and preparation method thereof | |
| CN104498369B (en) | Trichoderma koningii and bacterial agent containing same and application thereof in prevention of cylindrocarpon destructans | |
| CN101781565B (en) | Multifunctional biological agent containing Trichoderma pseudokiningii | |
| CN103194405B (en) | Growth-promoting bacteria for promoting ginger growth and preventing and controlling continuous cropping ginger soil-borne wilt and microorganism organic fertilizer produced from growth-promoting bacteria | |
| CN101759493B (en) | Biological disease-preventing organic fertilizer and preparation method thereof | |
| CN104928201A (en) | Bacillus amyloliquefaciens HN-11 and microbial inoculums thereof | |
| CN106966806A (en) | A kind of edible fungi residue living soil fertility regulation compound and preparation method thereof | |
| CN112725213B (en) | Arthrobacter and application thereof as decomposing agent for decomposing vegetable straw | |
| CN106242829A (en) | A kind of overcome the soil remediation fertilizer of Radix Salviae Miltiorrhizae continuous cropping obstacle, preparation method and its usage | |
| CN107603920A (en) | A kind of composite bacteria agent and its application in fermented organic waste increases numerous probiotics production biological organic fertilizer | |
| CN104774772A (en) | Straw decomposing fungicide with fertility increasing effect and application of fungicide | |
| CN113817615A (en) | Composite biocontrol microbial inoculum produced by fermenting Shuanghuanglian decoction dregs and application thereof | |
| CN103141517B (en) | Paenibacillus terrae biological agent and application thereof in agriculture | |
| CN106380269A (en) | Soil remediation organic fertilizer for overcoming watermelon replantation obstacle, preparation method thereof and application thereof | |
| CN109320355A (en) | A kind of saliferous bioactivity conditioner of improvement soil in protected field and its application | |
| CN102219613B (en) | Green multifunctional compound ecological organic liquid fertilizer and processing method thereof | |
| CN106365729A (en) | Soil remediation organic fertilizer for overcoming panax notoginseng continuous cropping obstruction, preparation method and application of soil remediation organic fertilizer | |
| CN103980020B (en) | The biological organic fertilizer that a kind of decomposing microbial inoculum and natural organic matter material produce | |
| CN104163733B (en) | A kind of organic compost of balcony vegetable plantation containing chinaberry element and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20121114 Termination date: 20141127 |
|
| EXPY | Termination of patent right or utility model |