CN107649493B - Preparation method of biological matrix loaded with strains and garbage treatment method - Google Patents
Preparation method of biological matrix loaded with strains and garbage treatment method Download PDFInfo
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- CN107649493B CN107649493B CN201711045403.8A CN201711045403A CN107649493B CN 107649493 B CN107649493 B CN 107649493B CN 201711045403 A CN201711045403 A CN 201711045403A CN 107649493 B CN107649493 B CN 107649493B
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- 238000000034 method Methods 0.000 title claims abstract description 27
- 238000002360 preparation method Methods 0.000 title claims abstract description 16
- 239000007788 liquid Substances 0.000 claims abstract description 123
- 239000004743 Polypropylene Substances 0.000 claims abstract description 107
- -1 polypropylene Polymers 0.000 claims abstract description 107
- 229920001155 polypropylene Polymers 0.000 claims abstract description 107
- 239000000463 material Substances 0.000 claims abstract description 64
- 238000005187 foaming Methods 0.000 claims abstract description 63
- 239000010806 kitchen waste Substances 0.000 claims abstract description 35
- 238000005507 spraying Methods 0.000 claims abstract description 27
- 238000003756 stirring Methods 0.000 claims abstract description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 18
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- 239000006261 foam material Substances 0.000 claims description 41
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 22
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- 239000000758 substrate Substances 0.000 claims description 6
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- 241000894007 species Species 0.000 claims description 4
- 239000000243 solution Substances 0.000 claims 8
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims 1
- 229910052799 carbon Inorganic materials 0.000 claims 1
- 238000011068 loading method Methods 0.000 abstract description 6
- 230000009467 reduction Effects 0.000 abstract description 3
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- QQONPFPTGQHPMA-UHFFFAOYSA-N propylene Natural products CC=C QQONPFPTGQHPMA-UHFFFAOYSA-N 0.000 description 3
- 125000004805 propylene group Chemical group [H]C([H])([H])C([H])([*:1])C([H])([H])[*:2] 0.000 description 3
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09B—DISPOSAL OF SOLID WASTE NOT OTHERWISE PROVIDED FOR
- B09B3/00—Destroying solid waste or transforming solid waste into something useful or harmless
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- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Processing Of Solid Wastes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides a preparation method of a biological matrix for loading strains and a garbage treatment method, wherein the preparation method of the biological matrix for loading the strains is characterized by comprising the following steps of: adding a biological matrix into the bin body, spraying water to wet the biological matrix, and then spraying liquid strains on the wetted biological matrix or soaking the wetted biological matrix with the liquid strains to obtain the strain-loaded biological matrix, wherein the biological matrix is a polypropylene foaming material. The garbage treatment method adopting the biological matrix loaded with the strains comprises the following steps of sequentially adding the biological matrix, water, the liquid strains and kitchen waste to be treated into a bin body under the stirring condition, degrading the kitchen waste to be treated by the bin body under the preset temperature and the preset stirring program, and cleaning residues in the bin body after the bin body runs for a certain time. The preparation method of the biological matrix loaded with the strains and the garbage treatment method have the advantages of high efficiency, environmental protection and reduction of industrial cost.
Description
Technical Field
The invention belongs to the field of organic garbage treatment, and particularly relates to a preparation method of a biological matrix loaded with strains and a garbage treatment method.
Background
The kitchen waste refers to food processing leftovers and edible residues generated in catering operation and resident life, and is divided into pre-meal waste, namely, the residues obtained by processing food materials before cooking, including vegetable leaves, fur and the like; the food waste after meal comprises leftover, soup residue, snack, starch, protein, and certain amount of paper towel. The kitchen waste is large in production amount, is dispersed, has high organic matter content, is extremely easy to decay and deteriorate, and is not suitable for long-distance transportation, so that the kitchen waste is generally required to be intensively and quickly subjected to reduction treatment nearby a production place, the volume of the kitchen waste is reduced, and subsequent transportation and treatment are facilitated.
At present, organic garbage is mainly subjected to aerobic fermentation decomposition treatment by microorganisms. Wherein, the microorganism needs to be loaded by adopting a biological matrix, and the biological matrix mainly adopts organic matters such as buckwheat hulls, bran and the like.
However, since the existing garbage disposal equipment needs to fully mix the biological garbage and the strains by stirring and the like, the organic substrates such as buckwheat hulls, bran and the like are broken after being used for about two months under continuous stirring. And the matrix is organic matter, which can be decomposed by the strains, and can play a role in degrading the garbage after losing the capabilities of introducing oxygen and bearing the strains. In addition, a large amount of broken buckwheat hulls and sticky substances are mixed in sewage generated by decomposing kitchen waste by the organic matter matrix, so that the sewage has high precipitability, and generated sediment is stubborn and difficult to wash and dredge. Meanwhile, the organic substance matrix needs to be replaced after being used for two months, and the produced waste cannot be utilized by equipment users and is difficult to treat. Therefore, the time from the input to the abandonment of the organic substance matrix is short, the organic substance matrix cannot be fully utilized, the efficiency of treating the kitchen waste is low, and the generated sewage is not friendly to the ecological environment.
Disclosure of Invention
The present invention has been made to solve the above problems, and an object of the present invention is to provide a method for producing a biological substrate carrying a bacterial strain and a method for treating garbage.
The invention provides a preparation method of a biological matrix loaded with strains, which is characterized by comprising the following steps: adding a biological matrix into the bin body, spraying water to wet the biological matrix, and then spraying liquid strains on the wetted biological matrix or soaking the wetted biological matrix with the liquid strains to obtain the strain-loaded biological matrix, wherein the biological matrix is a polypropylene foaming material.
The preparation method of the biological matrix loaded with the strains provided by the invention can also have the following characteristics: wherein the polypropylene foaming material comprises a tubular polypropylene foaming material, a hexagonal polypropylene foaming material and a cylindrical polypropylene foaming material, the diameter of the polypropylene foaming material is 3-15 mm, the length of the polypropylene foaming material is 3-15 mm, and the specific surface area of the polypropylene foaming material is 5000m2/m3～9000m2/m3Apparent specific gravity of 0.5g/cm3～0.95g/cm3。
The preparation method of the biological matrix loaded with the strains provided by the invention can also have the following characteristics: the liquid strain is a mixed solution of bacillus subtilis liquid, bacillus licheniformis liquid, bacillus amyloliquefaciens liquid and yeast liquid, the volume ratio of the yeast liquid to the bacillus subtilis liquid to the bacillus licheniformis liquid to the bacillus amyloliquefaciens liquid is 1:2:2:2, the bacteria concentrations of the bacillus subtilis liquid, the bacillus licheniformis liquid, the bacillus amyloliquefaciens liquid and the yeast liquid are all 1.5 multiplied by 109/ml, the widths of single bacteria cells in the bacillus subtilis liquid, the bacillus licheniformis liquid and the bacillus amyloliquefaciens liquid are all 0.7-0.8 mu m, the lengths of the single bacteria cells in the bacillus subtilis liquid, the bacillus licheniformis liquid and the bacillus amyloliquefaciens liquid are all 2-3 mu m, the widths of the single bacteria cells in the yeast liquid are 0.7-0.8 mu m, and the lengths of the single bacteria cells in the yeast liquid are 5-10 mu m.
The preparation method of the biological matrix loaded with the strains provided by the invention can also have the following characteristics: wherein the spraying amount of the liquid strains is that the liquid strains are sprayed into the wetted biological matrix according to the proportion that 6L-10L of the liquid strains are added into each cubic meter of the biological matrix.
The invention provides a method for treating garbage by adopting a biological matrix loaded with strains, which is characterized by comprising the following steps of:
step 1, adding a biological matrix into a bin body, and spraying water into the bin body under the stirring condition until the biological matrix is wetted;
step 2, after water spraying is finished, spraying the liquid strains on the wetted biological matrix under the condition of stirring;
step 3, adding a certain amount of kitchen garbage to be treated into the bin body after the liquid strains are sprayed;
step 4, after the bin body runs for 24 hours under the preset temperature and the preset stirring program, finishing mass propagation of liquid strains in the bin body and carrying out biochemical treatment on the kitchen waste to be treated;
step 5, after the bin body runs for a certain time, cleaning the undecomposed residues in the bin body,
wherein, the biological matrix is made of polypropylene foaming material and comprises the following steps:
adding the biological matrix into the bin body, spraying water to wet the biological matrix, and then spraying liquid strains on the wetted biological matrix or soaking the wetted biological matrix with the liquid strains to obtain the strain-loaded biological matrix.
The method for treating garbage by adopting the biological matrix loaded with the strains provided by the invention can also have the following characteristics: in the step 1, the biological matrix comprises a tubular polypropylene foaming material, a hexagonal polypropylene foaming material and a cylindrical polypropylene foaming material, wherein the input mass ratio of the tubular polypropylene foaming material to the hexagonal polypropylene foaming material to the cylindrical polypropylene foaming material is 2:1: 1.
The method for treating garbage by adopting the biological matrix loaded with the strains provided by the invention can also have the following characteristics: wherein, in the step 2, liquid strains are sprayed into the wetted biological matrix according to the proportion that 6L-10L of liquid strains are added into each cubic meter of biological matrix.
The method for treating garbage by adopting the biological matrix loaded with the strains provided by the invention can also have the following characteristics: wherein, in the step 3, the kitchen waste to be treated is added into the bin body according to the proportion that 1 ton of kitchen waste to be treated is added into every 20L-30L of liquid strains.
The method for treating garbage by adopting the biological matrix loaded with the strains provided by the invention can also have the following characteristics: wherein, in step 4, the preset temperature is 50 ℃, the preset stirring program is that the stirring is performed for one minute in the forward direction, for one minute in the backward direction, and then the stirring is performed for 20 minutes.
Action and Effect of the invention
According to the preparation method of the strain-loaded biological matrix, disclosed by the invention, the strain is distributed on the polypropylene foaming material in a liquid strain spraying mode or a soaking mode, so that a large number of strains are uniformly attached to the surface of the polypropylene foaming material, and the decomposition rate of the garbage can be increased when the kitchen garbage is degraded.
Compared with organic matter matrixes such as buckwheat hulls and bran, the polypropylene foaming material is used as a biological matrix, and due to the characteristics of high compressive strength and difficulty in being decomposed by strains, the biological matrix cannot be broken after being used for a long time, sewage generated in the decomposition process of kitchen waste treatment has good fluidity, stubborn precipitates cannot be generated, and pipelines are easy to wash and clean. Meanwhile, the advantages ensure the requirement of internal oxygen introduction in the kitchen waste decomposition process, and the kitchen waste can be recycled, so that the resource utilization rate is improved, and the production cost is reduced.
In addition, the polypropylene foam material is usually provided with a large number of micropores on the surface, so that the polypropylene foam material is very suitable for the adhesion and continuous growth of strains, the strains are not easy to wash away by running water, and the microbial biochemical decomposition of the kitchen waste is facilitated.
Drawings
FIG. 1 is a photograph of a polypropylene foam in an example of the present invention;
FIG. 2 is a photograph of a fresh seed-laden biological matrix in an example of the invention;
fig. 3 is a picture of fresh buckwheat hulls in an embodiment of the invention;
FIG. 4 is a photograph of a biological substrate loaded with a bacterial species after 2 months of use in an example of the present invention;
fig. 5 is a picture of buckwheat hulls after 2 months of use in an example of the invention.
Detailed Description
In order to make the technical means and functions of the present invention easy to understand, the present invention is specifically described below with reference to the embodiments and the accompanying drawings.
< example >
The preparation method of the biological matrix for loading the strains adopts a polypropylene foaming material as the biological matrix and comprises the following steps:
adding the biological matrix into the cabin, spraying water to wet the biological matrix, and spraying liquid strains into the wetted biological matrix according to the proportion that 10L of liquid strains are added into each cubic meter of the biological matrix, thereby obtaining the strain-loaded biological matrix.
FIG. 1 is a photograph of a polypropylene foam in an example of the present invention.
In this embodiment, as shown in fig. 1, the polypropylene foam material includes a tubular polypropylene foam material, a hexagonal polypropylene foam material and a cylindrical polypropylene foam material, the input mass ratio of the tubular polypropylene foam material, the hexagonal polypropylene foam material and the cylindrical polypropylene foam material is 2:1:1, the tubular polypropylene foam material has a diameter of 10mm, a length of 10mm and a specific surface area of 9000m2/m3Apparent specific gravity of 0.95g/cm3(ii) a The diameter of the hexagonal polypropylene foaming material is 8mm, the length of the hexagonal polypropylene foaming material is 8mm,specific surface area of 7000m2/m3Apparent specific gravity of 0.72g/cm3(ii) a The diameter of the cylindrical polypropylene foaming material is 5mm, the length of the cylindrical polypropylene foaming material is 5mm, and the specific surface area of the cylindrical polypropylene foaming material is 5000m2/m3An apparent specific gravity of 0.51g/cm3。
The liquid strain is a mixed solution of bacillus subtilis liquid, bacillus licheniformis liquid, bacillus amyloliquefaciens liquid and yeast liquid, the volume ratio of the yeast liquid to the bacillus subtilis liquid to the bacillus licheniformis liquid to the bacillus amyloliquefaciens liquid is 1:2:2:2, the bacteria concentration of the bacillus subtilis liquid to the bacillus licheniformis liquid to the bacillus amyloliquefaciens liquid is 1.5 multiplied by 109/ml, the width of each single bacterial cell in the bacillus subtilis liquid, the bacillus licheniformis liquid and the bacillus amyloliquefaciens liquid is 0.7 mu m, the length of each single bacterial cell in the yeast liquid is 2 mu m, and the width of each single bacterial cell in the yeast liquid is 0.7 mu m and the length of each single bacterial cell in the yeast liquid is 5 mu m.
The method for treating garbage by adopting the biological matrix loaded with the strains comprises the following steps:
step 1, adding the biological matrix into a bin body, and spraying water into the bin body under the stirring condition until the biological matrix is wetted (taking the water discharged from a filter screen at the bottom of the bin body as the standard).
And 2, after water spraying is finished, spraying the liquid strains on the wetted biological matrix according to the proportion of adding 6-10L of the liquid strains into each cubic meter of the biological matrix under the stirring condition.
And 3, after the liquid strains are sprayed, adding the kitchen waste to be treated into the bin body according to the proportion that 1 ton of the kitchen waste to be treated is added into every 20-30L of the liquid strains (the amount of the kitchen waste to be treated can be determined according to the capacity and other conditions of the bin body for treating the garbage).
And 4, carrying out forward stirring for one minute and backward stirring for one minute at the temperature of 50 ℃ in the bin body, standing for 20 minutes, and carrying out reciprocating operation for 24 hours to finish mass propagation of liquid strains in the bin body and carry out biochemical treatment on the kitchen waste to be treated.
And 5, adding new liquid strains and new kitchen waste to be treated into the bin body (according to the actual requirement), and cleaning the residue which cannot be decomposed in the bin body after the bin body runs for a period of time.
Wherein, the biological matrix is made of polypropylene foaming material and comprises the following steps:
adding the biological matrix into the cabin, spraying water to wet the biological matrix, and spraying liquid strains on the wetted biological matrix according to the proportion that 10L of liquid strains are added into each cubic meter of the biological matrix, thereby obtaining the strain-loaded biological matrix.
In this embodiment, the polypropylene foam material includes a tubular polypropylene foam material, a hexagonal polypropylene foam material and a cylindrical polypropylene foam material, the mass ratio of the tubular polypropylene foam material to the hexagonal polypropylene foam material to the cylindrical polypropylene foam material is 2:1:1, the tubular polypropylene foam material has a diameter of 10mm, a length of 10mm and a specific surface area of 9000m2/m3Apparent specific gravity of 0.95g/cm3(ii) a The diameter of the hexagonal polypropylene foaming material is 8mm, the length of the hexagonal polypropylene foaming material is 8mm, and the specific surface area of the hexagonal polypropylene foaming material is 7000m2/m3Apparent specific gravity of 0.72g/cm3(ii) a The diameter of the cylindrical polypropylene foaming material is 5mm, the length of the cylindrical polypropylene foaming material is 5mm, and the specific surface area of the cylindrical polypropylene foaming material is 5000m2/m3An apparent specific gravity of 0.51g/cm3。
The liquid strain is a mixed solution of bacillus subtilis liquid, bacillus licheniformis liquid, bacillus amyloliquefaciens liquid and yeast liquid, the volume ratio of the yeast liquid to the bacillus subtilis liquid to the bacillus licheniformis liquid to the bacillus amyloliquefaciens liquid is 1:2:2:2, the bacteria concentration of the bacillus subtilis liquid to the bacillus licheniformis liquid to the bacillus amyloliquefaciens liquid is 1.5 multiplied by 109/ml, the width of each single bacterial cell in the bacillus subtilis liquid, the bacillus licheniformis liquid and the bacillus amyloliquefaciens liquid is 0.7 mu m, the length of each single bacterial cell in the yeast liquid is 2 mu m, and the width of each single bacterial cell in the yeast liquid is 0.7 mu m and the length of each single bacterial cell in the yeast liquid is 5 mu m.
In this embodiment, the input mass of the kitchen waste to be treated is 500 kg, and after the bin body operates for 24 hours, the degradation rate of the kitchen waste to be treated reaches 90% under the action of the biological matrix loaded with the strains.
Control experiment: the operation is repeated by adopting buckwheat hulls as biological matrixes, but the input mass of the kitchen garbage to be treated is 420 kg, and after the same time, the degradation rate of the garbage to be treated reaches 90%.
Fig. 2 is a picture of fresh seed-laden biological matrix in an example of the invention, and fig. 3 is a picture of fresh buckwheat hulls in an example of the invention.
As shown in fig. 2, the biological matrix is a polypropylene foam material, most of which is tubular (hexagonal and cylindrical polypropylene foam materials are solid materials and are located below the tubular polypropylene foam material), a large number of micropores exist on the surface of the biological matrix (polypropylene foam material), a large number of strains are loaded on the surface, and a large number of gaps are formed between the accumulated strain-loaded biological matrices. As shown in fig. 3, the buckwheat hulls are relatively intact and fluffy when stacked together.
Fig. 4 is a picture of the bio-matrix loaded with the strain after 2 months of use in the example of the present invention, and fig. 5 is a picture of buckwheat hulls after 2 months of use in the example of the present invention.
As shown in FIG. 4, the bacterial-loaded biomatrix after 2 months of use was not broken, the shape was not substantially changed (hexagonal and cylindrical biomatrix was underneath the tubular biomatrix), and many voids remained between each other, and the light-colored portion in FIG. 4 was animal large bones that did not completely degrade, and slowly degraded over time. As shown in figure 5, after 2 months of use, most of the buckwheat hulls are broken and tightly adhered to the undegraded garbage, no gap exists, and the buckwheat hulls cannot be recycled.
Effects and effects of the embodiments
According to the preparation method of the biological matrix loaded with the strains, the strains are distributed on the polypropylene foaming material in a liquid strain spraying mode, so that a large number of strains are uniformly attached to the surface of the polypropylene foaming material, and the decomposition rate of the garbage can be increased when the kitchen garbage is degraded.
Compared with organic matter matrixes such as buckwheat hulls and bran, the polypropylene foaming material is used as a biological matrix, and due to the characteristics of high compressive strength and difficulty in being decomposed by strains, the biological matrix cannot be broken after being used for a long time, sewage generated in the decomposition process of kitchen waste treatment has good fluidity, stubborn precipitates cannot be generated, and pipelines are easy to wash and clean. Meanwhile, the advantages ensure the requirement of internal oxygen introduction in the kitchen waste decomposition process, and the kitchen waste can be recycled, so that the resource utilization rate is improved, and the production cost is reduced.
In addition, the polypropylene foaming material comprises a tubular polypropylene foaming material, a hexagonal polypropylene foaming material and a cylindrical polypropylene foaming material, the polypropylene foaming material can roll in the stirring process due to the special shape and the large apparent specific gravity, and the large specific surface area of the polypropylene foaming material can increase the attached surface area of strains and the contact area of the strains and the garbage to be treated, so that the complete biochemical degradation of the kitchen garbage is facilitated. Meanwhile, the surface of the polypropylene foam material is provided with a large number of micropores, so that the polypropylene foam material is very suitable for the adhesion and continuous growth of strains, the strains are not easy to wash away by running water, and the microbial biochemical decomposition of the kitchen waste is facilitated. In the embodiment, the polypropylene foaming materials with three shapes and different sizes are used as the biological matrixes, so that the biological matrixes can be better mixed with garbage with different shapes and sizes in the same batch in the biochemical garbage treatment process after adsorbing the strains, so that the strains can be more easily and fully contacted with the garbage, the biochemical degradation rate of the kitchen garbage is increased, and the kitchen garbage is basically and completely degraded.
Furthermore, because the weight of the polypropylene foaming material is light, when the kitchen garbage contains difficultly decomposed substances such as animal bones and the like which are not easy to decompose, the difficultly decomposed substances and the biological matrix are easily separated in a screening mode, so that the operation is simpler when the biological matrix is taken out for recycling, and the workload of manual sorting is reduced.
According to the method for treating garbage by using the biological matrix loaded with the strains, after the buckwheat hulls are used, the buckwheat hulls lose the functions of supporting and introducing oxygen due to stirring, crushing, loss and the like, and the strains are slowly decomposed after the oxygen is not provided, so that the buckwheat hulls are not finally decomposed. The biological matrix loaded with the strain has obvious effect after being used for the same time, and keeps the shape, so that the biological matrix still has the functions of supporting and oxygen supplying, and the biological matrix (polypropylene foaming material) loaded with the strain can be recycled.
Therefore, the preparation method of the biological matrix loaded with the strains and the garbage treatment method have the advantages of high efficiency, environmental protection and reduction of industrial cost.
The above embodiments are preferred examples of the present invention, and are not intended to limit the scope of the present invention.
In this embodiment, the bin body for processing biochemical garbage is adopted to spray liquid strains on the biological matrix, so that the garbage to be processed can be directly added after the biological matrix loaded with the strains is obtained.
In this embodiment, the preparation method of the biological matrix for loading strains is to add the biological matrix into the cabin, then spray the liquid strains on the wetted biological matrix according to the proportion of adding 10L of the liquid strains in each cubic meter of the biological matrix, thereby obtaining the biological matrix for loading the strains, but in the invention, the biological matrix can also be added into the cabin and sprayed with water to wet the biological matrix, and then spray the liquid strains on the wetted biological matrix according to the proportion of adding 6L-10L of the liquid strains in each cubic meter of the biological matrix or soak the wetted biological matrix with the liquid strains according to the proportion of adding 6L-10L of the liquid strains in each cubic meter of the biological matrix, thereby obtaining the biological matrix for loading the strains.
In this example, the polypropylene foams as the biomatrix include tubular polypropylene foams having a diameter of 10mm, a length of 10mm and a specific surface area of 9000m, hexagonal polypropylene foams and cylindrical polypropylene foams2/m3Apparent specific gravity of 0.95g/cm3(ii) a Hexagon polyThe diameter of the propylene foaming material is 8mm, the length of the propylene foaming material is 8mm, and the specific surface area of the propylene foaming material is 7000m2/m3Apparent specific gravity of 0.72g/cm3(ii) a The diameter of the cylindrical polypropylene foaming material is 5mm, the length of the cylindrical polypropylene foaming material is 5mm, and the specific surface area of the cylindrical polypropylene foaming material is 5000m2/m3An apparent specific gravity of 0.51g/cm3The width and the length of each single thallus cell in the bacillus subtilis liquid, the bacillus licheniformis liquid and the bacillus amyloliquefaciens liquid are respectively 0.7 mu m and 2 mu m, and the width and the length of each single thallus cell in the yeast liquid are respectively 0.7 mu m and 5 mu m, but in the invention, the diameters of the tubular polypropylene foaming material, the hexagonal polypropylene foaming material and the cylindrical polypropylene foaming material can also be 3 mm-15 mm, the lengths can also be 3 mm-15 mm, and the specific surface area can also be 5000m2/m3～9000m2/m3The apparent specific gravity may be 0.5g/cm3～0.95g/cm3The width of single thallus cell in bacillus subtilis liquid, bacillus licheniformis liquid and bacillus amyloliquefaciens liquid can be 0.7-0.8 μm, the length can be 2-3 μm, the width of single thallus cell in yeast liquid can be 0.7-0.8 μm, and the length can be 5-10 μm.
Claims (7)
1. A preparation method of a biological matrix loaded with strains, which is used for treating kitchen waste in a bin body capable of stirring, is characterized by comprising the following steps:
adding a biological matrix into a bin body, spraying water to wet the biological matrix, spraying liquid strains on the wetted biological matrix or soaking the wetted biological matrix with the liquid strains to obtain a strain-loaded biological matrix,
wherein the biological matrix is a polypropylene foaming material,
the diameter of the polypropylene foaming material is 3 mm-15 mm, the length of the polypropylene foaming material is 3 mm-15 mm, the polypropylene foaming material comprises a tubular polypropylene foaming material, a hexagonal polypropylene foaming material and a cylindrical polypropylene foaming material,
the input mass ratio of the tubular polypropylene foam material, the hexagonal polypropylene foam material and the cylindrical polypropylene foam material is 2:1:1, and the sizes of the tubular polypropylene foam material, the hexagonal polypropylene foam material and the cylindrical polypropylene foam material are different from each other,
the specific surface area of the polypropylene foaming material is 5000m2/m3～9000m2/m3Apparent specific gravity of 0.5g/cm3～0.95g/cm3。
2. The method of preparing a seed-supported biomatrix of claim 1, wherein:
wherein the liquid strain is a mixed solution of bacillus subtilis solution, bacillus licheniformis solution, bacillus amyloliquefaciens solution and yeast solution, the volume ratio of the yeast solution to the bacillus subtilis solution to the bacillus licheniformis solution to the bacillus amyloliquefaciens solution is 1:2:2:2,
the bacterial concentrations of the bacillus subtilis liquid, the bacillus licheniformis liquid, the bacillus amyloliquefaciens liquid and the yeast liquid are all 1.5 multiplied by 109The content of the active carbon is one/ml,
the width of each single thallus cell in the bacillus subtilis liquid, the bacillus licheniformis liquid and the bacillus amyloliquefaciens liquid is 0.7-0.8 mu m, the length of each single thallus cell is 2-3 mu m, the width of each single thallus cell in the yeast liquid is 0.7-0.8 mu m, and the length of each single thallus cell in the yeast liquid is 5-10 mu m.
3. The method of preparing a seed-supported biomatrix of claim 1, wherein:
wherein the spraying amount of the liquid strains is that the liquid strains are sprayed into the wetted biological matrix according to the proportion that 6L-10L of the liquid strains are added into each cubic meter of the biological matrix.
4. A method for treating kitchen waste by adopting a biological matrix loaded with strains is used for treating the kitchen waste in a bin body capable of stirring, and is characterized by comprising the following steps:
step 1, adding a biological matrix into a bin body, and spraying water into the bin body under the stirring condition until the biological matrix is wetted;
step 2, after the water spraying is finished, spraying the liquid strains on the wetted biological matrix under the stirring condition;
step 3, adding a certain amount of kitchen garbage to be treated into the bin body after the liquid strains are sprayed;
step 4, after the bin body runs for 24 hours under the preset temperature and the preset stirring program, finishing mass propagation of the liquid strains in the bin body and carrying out biochemical treatment on the kitchen waste to be treated;
step 5, after the bin body runs for a certain time, cleaning the undecomposed residues in the bin body,
wherein, the biological matrix is made of polypropylene foaming material and comprises the following steps:
adding a biological matrix into a bin body, spraying water to wet the biological matrix, spraying liquid strains on the wetted biological matrix or soaking the wetted biological matrix with the liquid strains to obtain a strain-loaded biological matrix,
in the step 1, the biological matrix is a polypropylene foam material with the diameter of 3 mm-15 mm and the length of 3 mm-15 mm, the polypropylene foam material comprises a tubular polypropylene foam material, a hexagonal polypropylene foam material and a cylindrical polypropylene foam material,
the input mass ratio of the tubular polypropylene foam material, the hexagonal polypropylene foam material and the cylindrical polypropylene foam material is 2:1:1, and the sizes of the tubular polypropylene foam material, the hexagonal polypropylene foam material and the cylindrical polypropylene foam material are different from each other,
the specific surface area of the polypropylene foaming material is 5000m2/m3～9000m2/m3Apparent specific gravity of 0.5g/cm3～0.95g/cm3。
5. The method for treating kitchen waste using a biological substrate loaded with bacterial species according to claim 4, wherein:
wherein, in the step 2, the liquid strain is sprayed into the wetted biological matrix according to the proportion that 6L-10L of the liquid strain is added into each cubic meter of the biological matrix.
6. The method for treating kitchen waste using a biological substrate loaded with bacterial species according to claim 4, wherein:
in the step 3, the kitchen waste to be treated is added into the bin body according to the proportion that 1 ton of the kitchen waste to be treated is added into every 20L-30L of the liquid strains.
7. The method for treating kitchen waste using a biological substrate loaded with bacterial species according to claim 4, wherein:
wherein, in the step 4, the preset temperature is 50 ℃,
the predetermined stirring program was one minute under forward stirring, one minute under reverse stirring, and then left to stand for 20 minutes.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| JPH07251147A (en) * | 1994-03-15 | 1995-10-03 | Sanyo Electric Co Ltd | Kitchen waste treatment device |
| CN1188150A (en) * | 1997-01-14 | 1998-07-22 | 筒中塑料工业股份公司 | Microbial fixed carrier for fluidized bed |
| CN2769275Y (en) * | 2004-02-13 | 2006-04-05 | 肖英佳 | Bionic filling for methane generation |
| CN1868919A (en) * | 2006-05-29 | 2006-11-29 | 南昌大学 | Suspension microorganism carrier and its preparation method |
| KR20130084712A (en) * | 2012-01-18 | 2013-07-26 | (주)바이오스타 | Carrier element for microorganism in which food waste fermentation apparatus |
| CN102747011A (en) * | 2012-05-11 | 2012-10-24 | 上海恒晔生物科技有限公司 | Mixing strain for treating kitchen waste, and method |
| CN103708625A (en) * | 2012-09-28 | 2014-04-09 | 无锡宜友机电制造有限公司 | Lightweight filler for floating biological filtration |
| CN104509688A (en) * | 2013-10-08 | 2015-04-15 | 俸祥仁 | Method of producing biological feed by fermenting banana stem and leaf through micro-organism |
| HK1217402A2 (en) * | 2014-11-10 | 2017-01-06 | Hong Kong Productivity Council | Mbbr carriers for high loading applications |
| CN104785508A (en) * | 2015-05-07 | 2015-07-22 | 上海德瑾绿水科技有限公司 | Buckwheat hull bacteria bed for biochemical degradation of kitchen wastes and preparation and using method thereof |
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