CN102533553B - Organic matter decomposition combined fungicide for rapidly degrading rice straws and application method thereof - Google Patents
Organic matter decomposition combined fungicide for rapidly degrading rice straws and application method thereof Download PDFInfo
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Abstract
The invention discloses an organic matter decomposition combined fungicide for rapidly degrading rice straws and an application method thereof, which are applied to in-place returning-to-field high-efficiency stable fertilization of the straws. The fungicide contains Trichoderma koningii CGMCC NO.3.4290, Bacillus licheniformis CGMCC NO.4835 and bacillus subtilis CGMCC NO.1.1414 with the fungus content ratio being 1:1:1. The fungicide is obtained after mixing a product obtained after solid fermentation culture is performed on the Trichoderma koningii with a culture obtained after two kinds of bacillus liquid are cultured and a product adsorbed by wheat bran. The fineness of the fungicide does not exceed 0.18mm, and the water content is lower than 30 percent. As a spore serves as a main existence form, the fungicide disclosed by the invention has strong adaptability, is suitable for spraying and spreading, is easy for mechanized operations, is capable of greatly reducing labor strength, shortening life cycle and accelerating the decomposition speed of the straws and is beneficial to large-scale use.
Description
Technical field
The present invention relates to be applied to organic material composting combination microbial inoculum and the application method thereof of going back on the spot the fertile a kind of fast degradation rice straw that uses of field efficient stable one-tenth of rice straw.
Background technology
Agricultural crop straw will produce nearly 200,000,000 tons of straw as a kind of renewable resources in the annual agricultural production process of China.Because natural decomposition needs the time longer, burning disposal is still in a large amount of uses, and it is the problem that receives much attention that the quick decomposition of straw also is used for going back the field processing.So the agricultural crop straw decomposition utilizes for solving following energy dilemma and environmental problem significant.
The Hunan is big agricultural province, and food crop are take double cropping paddy rice as main.Approximately 3,200 ten thousand tons of annual total stalk amounts, wherein rice straw is approximately 2,755 ten thousand tons." the straw-returning lifting soil organism " project and the Hunan Province's rice straw that start on the occasion of country fully utilize the period that the demonstration area creates, this institute intends developing adaptable and the high organic matter decomposing inoculant of degradation efficiency for south ecology, envrionment conditions and cropping system.The application of this technology and products thereof can be accelerated the speed of becoming thoroughly decomposed of stalk, improves the content of soil with organic matter, available potassium etc., can accelerate the rice nutrition supply, promote effective tillering, thereby increase plant height and effective fringe, promotes rice yield.Thereby effectively improved the stalk comprehensive utilization ratio, can obtain economic benefit, ecological benefits and social benefit preferably.
Summary of the invention
The object of the present invention is to provide a kind of be suitable for the stalk field on the spot also the field condition, rapidly and efficiently the decomposition stalk, have organic material composting combination microbial inoculum and the application method thereof of a kind of fast degradation rice straw of certain stability.
The technical solution adopted in the present invention
Contain the mould CGMCC NO.3.4290 of the bacteria containing amount proportioning healthy and free from worry wood of 1: 1: 1 in a kind of organic material composting combination microbial inoculum of fast degradation rice straw; Bacillus licheniformis (Bacillus licheniformis) CGMCC NO.4835; Subtilis CGMCC NO.1.1414; Described microbial inoculum is mixed by following three kinds of compositions and forms: the product of product, the culture after subtilis CGMCC NO.1.1414 liquid fermentation and culture and the wheat bran absorption of product, the culture after Bacillus licheniformis CGMCC NO.4835 liquid fermentation and culture and wheat bran absorption after the mould CGMCC NO.3.4290 of healthy and free from worry wood cultivates through liquid fermenting, solid fermentation successively.
The mould CGMCC NO.3.4290 of described healthy and free from worry wood cultivates 45-48h in 25-28 ℃ of solid fermentation, and in product, bacterial content is not less than 10
8Individual/g, described subtilis is cultivated 22-24h at 34-37 ℃, and described Bacillus licheniformis is after 45-55 ℃ of cultivation 36-40h, and with culture and wheat bran absorption, in product, bacterial content all is not less than 10 respectively
9Individual/g, described bacterial content comprise thalline and the total content of gemma.
In described microbial inoculum, bacterial content is not less than 2 * 10
8Individual/g, the microbial inoculum fineness is no more than 0.18mm, and water content is not higher than 30%.4. microbial inoculum according to claim 1, is characterized in that, the mould CGMCC NO.3.4290 of described healthy and free from worry wood solid fermentation detailed process is as follows:
At first after the mould CGMCC NO.3.4290 of described healthy and free from worry wood being cultivated through inclined-plane seed activation, shake-flask seed, the shake-flask seed nutrient solution is inoculated in the liquid fermentation tank substratum cultivates, incubation time 22-24h, temperature 25-28 ℃, rotating speed 120-150rpm; Air flow 0.4-0.5VVM; Inoculum size accounts for the 10-15% of culture volume, and in fermentor tank, the substratum loading amount accounts for the 60-70% of fermentor tank volume, and the liquid fermentation medium compound method is: sucrose 20g, yeast powder 0.2g, malt meal 3.0g, NaCl 0.1g, CaCl
22H
2O 0.1g, KH
2PO
41.0g, MgSO
47H
2O 0.5g, (NH
4)
2SO
40.5g adding distil water is to 1L, pH6.5,121 ℃, 0.1MPa autoclaving 30min;
Then inoculation accounts for the fermentation culture seed liquor of solid medium weight 10-15%, and 25-28 ℃ of solid fermentation cultivated 45-48h; The compound method of solid fermentation substratum is: Semen Maydis powder 10g, wheat bran 90g, (NH
4)
2SO
41.0g, KH
2PO
40.2g, MgSO
47H
2O 0.05g, 80mL distilled water, 121 ℃, 0.1MPa autoclaving 30min, in solid-state fermentation tank, the loading amount of substratum is the 60-70% of volume.
After described Bacillus licheniformis CGMCC NO.4835 cultivates through inclined-plane seed activation, shake-flask seed, the shake-flask seed nutrient solution is inoculated in the liquid fermentation tank substratum cultivates, the compound method of liquid fermentation medium used is: starch 5.0g, dextrin 20g, soybean cake powder 25g, yeast powder 3.0g, CaCO
312g, (NH
4)
2SO
410g, NaCl 4.0g, KH
2PO
40.2g adding distil water is to 1L, pH7.2-7.4,121 ℃, 0.1MPa autoclaving 30min; Incubation time 36-40h, temperature 45-55 ℃, rotating speed 180-200rpm, air flow 0.8-1.0VVM, inoculum size accounts for the 8-10% of culture volume, and in fermentor tank, the liquid amount of substratum is the 55-65% of fermentor tank volume.
After described subtilis CGMCC NO.1.1414 cultivates through inclined-plane seed activation, shake-flask seed, the shake-flask seed nutrient solution is inoculated in the liquid fermentation tank substratum cultivates, the compound method of liquid fermentation medium used is: glucose 5.0g, sucrose 3.0g, soybean cake powder 15g, yeast powder 10g, peptone 5.0g, CaCO
31.0g, FeCl
36H
2O 0.1g, KH
2PO
44.0g, MgSO
47H
2O 0.5g, MnSO
4H
2O 0.062g, adding distil water be to 1L, pH7.2,121 ℃, 0.1MPa autoclaving 30min; Incubation time 22-24h, temperature 34-37 ℃, rotating speed 180-200rpm, air flow 0.8-1.0VVM, inoculum size accounts for the 8-10% of culture volume, and in fermentor tank, the liquid amount of substratum is the 55-65% of fermentor tank volume.
In above-mentioned microbial inoculum preparation process, during wheat bran absorption, its part by weight accounts for 70-80%.
Described microbial inoculum is used for improving Rice Characters and output; Specifically add in rice straw, be used for the content that the acceleration rice straw becomes thoroughly decomposed, improves soil with organic matter, available potassium, accelerate the rice nutrition supply, promote effective tillering, thereby increase plant height and effective fringe, promote rice yield.
Principle and the beneficial effect of combination bacterial strain of the present invention are: the composition that the present invention is directed to stalk, take multiple lignoenzyme vigor, cellulase activity, hemicellulose enzyme activity and straw degradative rate as detecting index, choose especially the mould CGMCC NO.3.4290 of healthy and free from worry wood; Bacillus licheniformis CGMCC NO.4835; Subtilis CGMCC NO.1.1414.Again the product of three kinds of strain culturing is mixed with the microbial inoculum that forms and is applied to straw also field field plot trial on the spot.Due to after the stalk structure is studied, it is found that xylogen is combined with the covalent linkage form with hemicellulose, cellulosic molecule is embedded in wherein, form a kind of natural cover for defense, make cellulase etc. be difficult for contacting with cellulosic molecule, and the complicacy of water-insoluble, the chemical structure of xylogen has caused the refractory organics of stalk.Therefore want thorough degraded cellulose, at first must solve the degradation problem of xylogen.At first utilize that healthy and free from worry wood is mould decomposes the composition wax and xylogen is preferentially degraded for stalk is outer difficult; Actively construct by cellulose-binding degradation bacteria (Bacillus licheniformis and subtilis) strain combinations that improves straw class straw decomposition efficient again.
Be comprised of xylogen, Mierocrystalline cellulose, hemicellulose etc. for stalk, the present invention will secrete that the healthy and free from worry wood of highly active lignoenzyme, cellulase and hemicellulase is mould, Bacillus licheniformis and bacillus subtilis strain make up.Because this strain combination has multiple bacterial strain, have certain stability, and all bacterium exist with the gemma form all, growth conditions is applicable wide, and easily Survival Reproduction, especially have very strong synergetic property on utilization of carbon source, therefore is easy to improve straw decomposing efficient.This straw degradative microbial inoculum is combined in 14 days of degrading straw, and it is stable that the pH value keeps, and lignoenzyme, cellulase activity and living bacteria count amount remain at 10
7Individual/more than g, the straw degradative rate constantly increases, and illustrates that this straw degradative microbial inoculum has adaptation and regulating power very by force to pH, can maintain property stablize, and also has certain stability simultaneously on Function.This microbial inoculum is take spore as main existence form, and fineness is in the 0.18mm levels, strong adaptability, in being applied to the returning field into fertilizer on the spot of rice straw, be suitable for spreading fertilizer over the fields, spraying, be easy to mechanized operation, can greatly reduce labour intensity, shorten life cycle, accelerate straw decomposing, be conducive to extensive use.
Bacillus licheniformis in strain combination of the present invention (Bacillus licheniformis) preserving number is: CGMCC NO.4835, it is the bacterial strain by the applicant oneself screening, and carried out preservation on May 9th, 2011 at China Committee for Culture Collection of Microorganisms's common micro-organisms center of the Institute of Microorganism, Academia Sinica that is positioned at No. 3, No. 1, North Star West Road, Chaoyang District, BeiJing, China city institute, and applied for that patent, application number are 201110166962.0.
The mould CGMCC NO.3.4290 of subtilis CGMCC NO.11414 and healthy and free from worry wood all buys the institute of microbiology in the Chinese Academy of Sciences.
Embodiment
Be intended to further illustrate the present invention below in conjunction with embodiment, and unrestricted the present invention.
Embodiment 1 microbial inoculum preparation of the present invention
1) the mould CGMCC NO.3.4290 of healthy and free from worry wood is cultivated through inclined-plane seed, shake-flask seed after to the liquid fermentation tank fermentation culture, finally cultivate in solid-state fermentation tank and produce spore, the compound method of inclined-plane seed culture used medium is: peptone 6.0g, potato 200g, NaCl 2.0g, MgSO
41.0g, sucrose 20g, KH
2PO
41.0g agar 18-20g, adding distil water are to 1L, pH is natural, 121 ℃, and 0.1MPa autoclaving 30min.Inclined-plane seed culture time 30-36h, temperature 25-28 ℃.Inclined-plane seed inoculation two to three encircles to shake-flask seed cultivates, and the substratum loading amount is 100mL in 500mL triangle shaking flask.The compound method of shake-flask seed used medium is: soybean cake powder 1.5g, sucrose 20g, MgSO
41.5g, KH
2PO
43.0g adding distil water is to 1L, pH is natural, 121 ℃, and 0.1MPa autoclaving 30min, incubation time 22-24h, temperature 25-28 ℃, rotating speed 120-150rpm cultivates in the liquid fermentation tank substratum, incubation time 22h, 28 ℃ of temperature, rotating speed 150rpm, air flow 0.5VVM.The liquid fermentation medium compound method is: sucrose 20g, yeast powder 0.2g, malt meal 3.0g, NaCl 0.1g, CaCl
22H
2O 0.1g, KH
2PO
41.0g, MgSO
47H
2O 0.5g, (NH
4)
2SO
40.5g adding distil water is to 1L, pH6.5,121 ℃, 0.1MPa autoclaving 30min; Inoculum size accounts for 15% of culture volume, and in fermentor tank, the substratum loading amount accounts for 70% of volume.
2) then inoculate the liquid fermentation and culture seed liquor that accounts for solid medium weight 15%, 25 ℃ of solid fermentations are cultivated 48h; The compound method of solid medium is: Semen Maydis powder 10g, wheat bran 90g, (NH
4)
2SO
41.0g, KH
2PO
40.2g, MgSO
47H
2O0.05g, 80mL distilled water, 121 ℃, 0.1MPa autoclaving 30min.In solid-state fermentation tank, the loading amount of substratum is 70% of volume.In product, bacterial content is in 3-6 * 10
8Individual/g, bacterial content comprise thalline and the total content of gemma.
3) after Bacillus licheniformis CGMCC NO.4835 first cultivates through inclined-plane seed, shake-flask seed to fermentation culture, wherein the compound method of Bacillus licheniformis CGMCC NO.4835 inclined-plane seed culture used medium is: glucose 4.0g, yeast extract paste 4.0g, malt meal 10.0g, CaCO
32.0g agar 20g, adding distil water be to 1L, pH7.2, incubation time 24-26h, temperature 45-55 ℃.Inclined-plane seed inoculation one encircles to shake-flask seed cultivates, and the substratum loading amount is 100mL in 500mL triangle shaking flask.The compound method of used medium is: starch 30.0g, soybean cake powder 5.0g, yeast extract paste 5.0g, CaCO
35.0g, (NH
4)
2SO
44.0g, NaCl 5.0g, KH
2PO
40.12g adding distil water is to 1L, pH7.2,121 ℃, 0.1MPa autoclaving 30min.Incubation time 24-30h, temperature 45-55 ℃, rotating speed 200-220rpm.Then inoculate the seed culture fluid that accounts for fermention medium volume 8% and cultivate 36h in fermention medium, 55 ℃ of temperature, rotating speed 200rpm, air flow 0.8VVM.In fermentor tank, the liquid amount of substratum is 65%.The compound method of fermention medium used is: starch 5.0g, dextrin 20g, soybean cake powder 25g, yeast powder 3.0g, CaCO
312g, (NH
4)
2SO
410g, NaCl 4.0g, KH
2PO
40.2g adding distil water is to 1L, pH7.2,121 ℃, 0.1MPa autoclaving 30min.
With culture and the wheat bran absorption of fermentation culture, in product, bacterial content is all in 4-6 * 10
9Individual/g, bacterial content comprise thalline and the total content of gemma.
4) after subtilis CGMCC NO.1.1414 first cultivates through inclined-plane seed culture, shake-flask seed to fermentation culture, the compound method of inclined-plane used seed culture medium is: extractum carnis 3.0 peptone 10.0 sodium-chlor 5.0, adding distil water is to 1L, pH7.2,121 ℃, 0.1MPa autoclaving 30min; Incubation time 22-24h, temperature 34-37 ℃.Inclined-plane seed inoculation one encircles to shake-flask seed cultivates, and the substratum loading amount is 100mL in 500mL triangle shaking flask.The compound method that shake-flask seed is cultivated used medium is: extractum carnis 5.0 yeast extract 5.0 peptone 10.0 glucose 5.0 sodium-chlor 5.0 distilled water 1000mlpH7.0,121 ℃, 0.1MPa autoclaving 30min.Incubation time 22-24h, temperature 34-37 ℃, rotating speed 200-220rpm.The shake-flask seed nutrient solution of inoculation 8% is to fermention medium, and in fermentor tank, the liquid amount of substratum is 65%.The compound method of fermention medium used is: glucose 5.0g, sucrose 3.0g, soybean cake powder 15g, yeast powder 10g, peptone 5.0g, CaCO
31.0g, FeCl
36H
2O 0.1g, KH
2PO
44.0g, MgSO
47H
2O 0.5g, MnSO
4H
2O 0.062g, adding distil water be to 1L, pH7.2,121 ℃, 0.1MPa autoclaving 30min.Incubation time 22h, 37 ℃ of temperature, rotating speed 200rpm, air flow 0.8VVM.With culture and the wheat bran absorption of fermentation culture, in product, bacterial content is all in 6-8 * 10
9Individual/g, bacterial content comprise thalline and the total content of gemma.
5) product after process liquid fermenting, solid fermentation are cultivated successively with the mould CGMCC NO.3.4290 of above-mentioned healthy and free from worry wood; With the microbial inoculum that obtains after the product of the product of culture after Bacillus licheniformis CGMCC NO.4835 liquid fermentation and culture and wheat bran absorption, culture after subtilis CGMCC NO.1.1414 liquid fermentation and culture and wheat bran absorption mixes.The part by weight of wheat bran absorption is 70%, the mould CGMCC NO.3.4290 of the bacteria containing amount proportioning healthy and free from worry wood of 1: 1: 1; Bacillus licheniformis CGMCC NO.4835; Subtilis CGMCC NO.1.1414; In microbial inoculum, bacterial content is 2 * 10
8Individual/g; The microbial inoculum fineness is 0.18mm, and water content is not higher than 30%.
Embodiment 2
Bacterial preparation process is with embodiment 1, be incubation time when cultivating in the mould CGMCC NO.3.4290 of healthy and free from worry wood liquid fermentation tank substratum be 24h, 25 ℃ of temperature, rotating speed 120rpm, air flow 0.4VVM.Inoculum size accounts for 10% of culture volume, and in fermentor tank, the substratum loading amount is 60%.Then inoculation accounts for the liquid culture seed liquor of solid medium weight 10%, and 28 ℃ of solid fermentations are cultivated 45h; In solid-state fermentation tank, the loading amount of substratum is 60% of volume.In product, bacterial content is in 3-6 * 10
8Individual/g, bacterial content comprise thalline and the total content of gemma.
Bacillus licheniformis CGMCC NO.4835, inoculation accounts for the seed culture fluid of fermention medium volume 10% and cultivate 40h in fermention medium, temperature 45 C, rotating speed 180rpm, air flow 1.0VVM.In fermentor tank, the liquid amount of substratum is 55%.With culture and wheat bran absorption, in product, bacterial content is all in 4-6 * 10
9Individual/g, bacterial content comprise thalline and the total content of gemma.
Subtilis CGMCC NO.1.1414, inoculation 10% shake-flask seed nutrient solution to fermention medium, incubation time 24h, 35 ℃ of temperature, rotating speed 180rpm, air flow 1.0VVM.In fermentor tank, the liquid amount of substratum is 55%.With culture and the wheat bran absorption of fermentation culture, in product, bacterial content is all in 6-8 * 10
9Individual/g, bacterial content comprise thalline and the total content of gemma.
The part by weight of wheat bran absorption is 80%, and in the microbial inoculum that obtains at last, bacterial content is 2 * 10
8Individual/g, the microbial inoculum fineness is 0.18mm, water content is not higher than 30%.
Embodiment 3 microbial inoculum straw degradative rate contrasts
The cultural method that the rice straw degradation rate is measured substratum is: fresh rice straw is cut into the long short stalk of 1cm, take the triangular flask that 20g adds 500mL, every bottle of following microbial inoculum and 100mL distilled water that adds again 50g mixes, nature pH value, 30min sterilizes under 121 ℃, 0.1MPa.
At first cellulose-degrading bacteria (being two kinds of genus bacillus of the present invention) being cultivated after 24h wheat bran absorption respectively at 34 ℃, to make concentration be 10
8The spore pulvis of individual/g mixes according to 1: 1 volume ratio, and the straw degradative rate is 39.6%, higher than single strain; And the present invention is composite with two kinds of cellulose-degrading bacterias and lignin-degrading bacteria, and the straw degradative rate brings up to 50.2%.Illustrate that the bacterial strain in this combination exists certain synergetic property, does not have Antagonism between them.
Strain combination microbial inoculum of the present invention is take the rice straw degradation rate as index, and its degradation rate to rice straw is significantly improved than single bacterial classification, has reached 50.2%.
The application of embodiment 4 microbial inoculum of the present invention in the shortening rice straw becomes thoroughly decomposed the time
5 processing are established in test, 3 repetitions.Process 1: conventional fertilizer application+straw incorporation+close edge board board organic matter decomposing inoculant; Process 2: conventional fertilizer application+straw incorporation+microbial inoculum of the present invention; Process 3: conventional fertilizer application+straw incorporation+paddy continuous heavy rain board organic matter decomposing inoculant; Process 4: conventional fertilizer application+straw incorporation; Process 5: conventional fertilizer application; Late rice test site random alignment, residential quarter area 33.3M
2Test-results is to process 2 ratio processing 4 shortenings to become thoroughly decomposed the time, and processing 1 is excellent (seeing Table 1) with processing 3.
The table 1 different treatment straw degree record table that becomes thoroughly decomposed
Annotate: under general condition, the straw decomposition degree is qualitative comparison, during statistics, Huang, little Huang, brown Huang, black Huang in the stalk color are decided to be respectively 1,2,3,4 grade, musty in the stalk smell, ammonia flavor, vinosity, putrid taste are decided to be respectively 1,2,3,4 grade, hard, Microsoft in the feel softening degree, soft, rot to be decided to be respectively 1,2,3,4 grade, decomposition degree numerical value during during statistics, each is processed is three norms numerical value sum, the stalk of larger this processing of expression of numerical value rots faster, and the effect of becoming thoroughly decomposed is more obvious.
The application of embodiment 5 microbial inoculum of the present invention in Economic Character of Rice improves
With the same treatment group of case study on implementation 4.Processing 2 ratio processing 1 have increase with processing 3 every mu of average plant heights with effective fringe, and rice yield also has increase, and while and processing 4 and processing 5 significances of difference have economic benefit (seeing Table 2,3,4,5,6).
Table 2 different treatment economic characters table (unit: cm, ten thousand/667m
2, %, g, kg/667m
2)
Table 3 different treatment rate schedule (unit: strain/667m
2, kg/667m
2)
Table 4 variance analysis
| Source of variation | The free degree | Quadratic sum | All square | The F value | F0.05 | F0.01 |
| Between processing | 4 | 19.871 | 4.968 | 114.638 | 3.478 | 5.994 |
| Error | 10 | 0.433 | 0.043 | - | - | - |
| Total variation | 14 | 20.304 | - | - | - | - |
[0049]Table 5 multiple comparisons (LSD method)
Table 6 different treatment performance analysis (unit: unit/667m
2, kg/667m
2)
Embodiment 6 microbial inoculum working conditions of the present invention and using method
1. working conditions: the field optimum inoculation amount of this straw degradative combination microbial inoculum is 4 ‰, and namely every kilogram of rice straw need to be inoculated the 4g degradation bacterial agent; When the land for growing field crops applies microbial inoculum, need to apply nitrogen and phosphorus.The applied amount of every kilogram of rice straw nitrogen is 12g, and the additional amount of phosphorus is 5g, and generally the more large rice straw that more is conducive to is degraded, and therefore can determine the phosphorus magnitude of recruitment according to practical situation.
2. using method: in conjunction with rice straw also when the field, spread fertilizer over the fields or spray microbial inoculum of the present invention.Consumption: every mu of 2-3 kilogram, spread fertilizer over the fields on rice straw or spray microbial inoculum of the present invention and get final product.Apply simultaneously nitrogenous fertilizer and phosphate fertilizer, then rice straw is ploughed under underground, also applicable to the rice straw fermentation of becoming thoroughly decomposed under condition of banking up.
Claims (2)
1. the organic material composting of fast degradation rice straw combination microbial inoculum, is characterized in that, contains the mould CGMCC NO.3.4290 of healthy and free from worry wood of bacteria containing amount proportioning 1 ︰ 1 ︰ 1 in described microbial inoculum; Bacillus licheniformis CGMCC NO.4835; Subtilis CGMCC NO.1.1414; Described microbial inoculum is mixed by following three kinds of compositions and forms: the product of product, the culture after subtilis CGMCC NO.1.1414 liquid fermentation and culture and the wheat bran absorption of product, the culture after Bacillus licheniformis CGMCC NO.4835 liquid fermentation and culture and wheat bran absorption after the mould CGMCC NO.3.4290 of healthy and free from worry wood cultivates through liquid fermenting, solid fermentation successively;
The mould CGMCC NO.3.4290 of described healthy and free from worry wood cultivates 45-48h in 25-28 ℃ of solid fermentation, and in product, bacterial content is not less than 10
8Individual/g, described subtilis is cultivated 22-24h at 34-37 ℃, and described Bacillus licheniformis is after 45-55 ℃ of cultivation 36-40h, and with culture and wheat bran absorption, in product, bacterial content all is not less than 10 respectively
9Individual/g, described bacterial content comprise thalline and the total content of gemma;
In described microbial inoculum, bacterial content is not less than 2 * 10
8Individual/g, the microbial inoculum fineness is no more than 0.18mm, and water content is not higher than 30%;
The mould CGMCC NO.3.4290 of described healthy and free from worry wood solid fermentation detailed process is as follows:
At first after the mould CGMCC NO.3.4290 of described healthy and free from worry wood being cultivated through inclined-plane seed activation, shake-flask seed, the shake-flask seed nutrient solution is inoculated in the liquid fermentation tank substratum cultivates, incubation time 22-24h, 25-28 ℃ of temperature, rotating speed 120-150rpm; Air flow 0.4-0.5VVM; Inoculum size accounts for the 10-15% of culture volume, and in fermentor tank, the substratum loading amount accounts for 60-70% of fermentor tank volume, and the liquid fermentation medium compound method is: sucrose 20g, yeast powder 0.2g, malt meal 3.0g, NaCl 0.1g, CaCl
22H
2O 0.1g, KH
2PO
41.0g, MgSO
47H
2O 0.5g, (NH
4)
2SO
40.5g adding distil water is to 1L, pH6.5,121 ℃, 0.1MPa autoclaving 30min; Then inoculation accounts for the fermentation culture seed liquor of solid medium weight 10-15%, and 25-28 ℃ of solid fermentation cultivated 45-48h; The compound method of solid fermentation substratum is: Semen Maydis powder 10g, wheat bran 90g, (NH
4)
2SO
41.0g, KH
2PO
40.2g, MgSO
47H
2O 0.05g, 80mL distilled water, 121 ℃, 0.1MPa autoclaving 30min, in solid-state fermentation tank, the loading amount of substratum is the 60-70% of volume;
After described Bacillus licheniformis CGMCC NO.4835 cultivates through inclined-plane seed activation, shake-flask seed, the shake-flask seed nutrient solution is inoculated in the liquid fermentation tank substratum cultivates, the compound method of liquid fermentation medium used is: starch 5.0g, dextrin 20g, soybean cake powder 25g, yeast powder 3.0g, CaCO
312g, (NH
4)
2SO
410g, NaCl 4.0g, KH
2PO
40.2g adding distil water is to 1L, pH7.2-7.4,121 ℃, 0.1MPa autoclaving 30min; Incubation time 36-40h, temperature 45-55 ℃, rotating speed 180-200rpm, air flow 0.8-1.0VVM, inoculum size accounts for the 8-10% of culture volume, and in fermentor tank, the liquid amount of substratum is the 55-65% of fermentor tank volume;
After described subtilis CGMCC NO.1.1414 cultivates through inclined-plane seed activation, shake-flask seed, the shake-flask seed nutrient solution is inoculated in the liquid fermentation tank substratum cultivates, the compound method of liquid fermentation medium used is: glucose 5.0g, sucrose 3.0g, soybean cake powder 15g, yeast powder 10g, peptone 5.0g, CaCO
31.0g, FeCl
36H
2O 0.1g, KH
2PO
44.0g, MgSO
47H
2O 0.5g, MnSO
4H
2O 0.062g, adding distil water be to 1L, pH7.2,121 ℃, 0.1MPa autoclaving 30min; Incubation time 22-24h, 34-37 ℃ of temperature, rotating speed 180-200rpm, air flow 0.8-1.0VVM, inoculum size accounts for the 8-10% of culture volume, and in fermentor tank, the liquid amount of substratum is 55-65% of fermentor tank volume;
During wheat bran absorption, its part by weight accounts for 70-80%.
2. the application method of microbial inoculum claimed in claim 1, is characterized in that, adds in rice straw, the acceleration rice straw becomes thoroughly decomposed, improves the content of soil with organic matter, available potassium, accelerate the rice nutrition supply, promote effective tillering, thereby increase plant height and effective fringe, promote rice yield.
Priority Applications (1)
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| CN102826661A (en) * | 2012-09-18 | 2012-12-19 | 苏州大学 | Treatment method of contaminated water body by promoting degrading bacteria to be fixedly planted at biological film |
| CN102845270B (en) * | 2012-09-29 | 2014-07-16 | 喻彬 | Organic rice cultivation method |
| CN103274771B (en) * | 2013-05-31 | 2015-11-25 | 上海创博生态工程有限公司 | A kind of straw decomposing inoculant and preparation method thereof |
| CN103525741A (en) * | 2013-08-12 | 2014-01-22 | 辽宁省微生物科学研究院 | Complex microbial inoculant for greenhouse vegetable production and preparation and application thereof |
| JP5866035B1 (en) * | 2015-01-08 | 2016-02-17 | アサヒグループホールディングス株式会社 | Effective tillering promotion method |
| CN104609995B (en) * | 2015-01-22 | 2017-05-24 | 河海大学 | Plant growth promoting bio-organic fertilizer for saline-alkali land |
| CN105296394A (en) * | 2015-11-04 | 2016-02-03 | 广西多得乐生物科技有限公司 | Microbial decomposition agent for animal wastes and straws and preparation method thereof |
| CN106635843B (en) * | 2017-02-08 | 2019-09-27 | 金华职业技术学院 | Fermentation medium, mushroom bran fermenting agent and fermentation process |
| CN107142210A (en) * | 2017-04-23 | 2017-09-08 | 贵州省烟草公司黔西南州公司 | A kind of compound method of hard stalk fermentation microbial inoculum |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007063129A2 (en) * | 2005-12-02 | 2007-06-07 | Novozymes A/S | Insolation of peptides , polypeptides and proteins |
| CN101288846A (en) * | 2008-05-09 | 2008-10-22 | 淮阴工学院 | Method for preprocessing and producing rice hull ash adsorbent using with microbe |
| WO2009009391A2 (en) * | 2007-07-06 | 2009-01-15 | Ls9, Inc. | Systems and methods for the production of fatty esters |
| CN102146341A (en) * | 2010-12-31 | 2011-08-10 | 东莞市天盛生物科技有限公司 | Straw decomposing inoculant |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1129683A (en) * | 1995-04-14 | 1996-08-28 | 北京兆元技术发展公司 | Multiple component organic composite liquid fertilizer and preparation thereof |
| CN101988040A (en) * | 2009-08-06 | 2011-03-23 | 南开大学 | Compound bactericide for efficient degradation of landscaping garbage and preparation method thereof |
| CN102174400A (en) * | 2011-01-27 | 2011-09-07 | 南昌大学 | Microbial degrading agent for decomposing cassava stalks and preparation method |
| CN102199564B (en) * | 2011-03-28 | 2012-11-21 | 武汉合缘绿色生物工程有限公司 | Composite microbial agent for direct decomposition of crop straws returned to field and preparation method thereof |
-
2011
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007063129A2 (en) * | 2005-12-02 | 2007-06-07 | Novozymes A/S | Insolation of peptides , polypeptides and proteins |
| WO2009009391A2 (en) * | 2007-07-06 | 2009-01-15 | Ls9, Inc. | Systems and methods for the production of fatty esters |
| CN101288846A (en) * | 2008-05-09 | 2008-10-22 | 淮阴工学院 | Method for preprocessing and producing rice hull ash adsorbent using with microbe |
| CN102146341A (en) * | 2010-12-31 | 2011-08-10 | 东莞市天盛生物科技有限公司 | Straw decomposing inoculant |
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