CN104403971A - Straw decomposing agent and preparation method thereof - Google Patents

Straw decomposing agent and preparation method thereof Download PDF

Info

Publication number
CN104403971A
CN104403971A CN201410712741.2A CN201410712741A CN104403971A CN 104403971 A CN104403971 A CN 104403971A CN 201410712741 A CN201410712741 A CN 201410712741A CN 104403971 A CN104403971 A CN 104403971A
Authority
CN
China
Prior art keywords
preparation
straw decomposing
decomposing inoculant
aspergillus niger
bacillus licheniformis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201410712741.2A
Other languages
Chinese (zh)
Inventor
蒋小春
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to CN201410712741.2A priority Critical patent/CN104403971A/en
Publication of CN104403971A publication Critical patent/CN104403971A/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00—Other organic fertilisers
    • C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention discloses a straw decomposing agent and a preparation method thereof. The preparation method comprises the following steps: culturing bacillus subtilis, bacillus licheniformis, liquor-making yeasts, aspergillus niger and trichoderma koningii respectively until the bacterial concentration is (0.1-1)*10<8>/mL, uniformly mixing according to a bacterial amount ratio, inoculating to sugarcane leaves according to a ratio of 1 to (10-20) for culturing at 25-30 DEG C for 5-10 days under the condition that the moisture content of the sugarcane leaves is 30%-50%, and finally grinding the sugarcane leaves to be 20-100-mesh to prepare the straw decomposing agent. According to the preparation method, the bacterial selection and ratio are reasonable, and the bacteria have a synergistic effect, can effectively decompose ingredients such as cellulose, hemicelluloses and lignin in straws and increase the decomposing speed, and can effectively decompose the straws within 15 days in general; the preparation method is simple and easy to control, the culture is mainly carried out at the room temperature, a high-price culture agent does not need to be added, the production cost can be reduced, and the promotion is facilitated.

Description

A kind of straw decomposing inoculant and preparation method thereof
Technical field
The present invention relates to a kind of straw decomposing inoculant and preparation method thereof.
Background technology
Stalk, refers to remaining cauline leaf part after the ripe threshing of the gramineae farm crop such as paddy rice, wheat, corn; Wherein the stalk of paddy rice is often called straw, rice ligusticumic, is commonly called as straw grass; The stalk of wheat is then called straw.China's recent two decades carrys out universal, various mechanicals abundant due to coal, electricity, Sweet natural gas, rural area is reduced the demand of stalk, a large amount of stalk be processed in order to a serious social concern, although law bans, but a lot of local peasant still direct burning straw in the fields, causes atmospheric pollution, fire, aircraft cannot the consequence such as Normal Take-Off And Landing.Along with the development of agriculture production, the utilization of stalk is made compost by original heap and is changed straw directly returning to field into.While being directly sprinkling upon Tanaka by stalk, use some efficient-decomposition composite floras---straw decomposing inoculant, can promote the decomposition of stalk inner cellulose, hemicellulose and xylogen, has certain effect to becoming thoroughly decomposed of stalk.But common straw decomposing inoculant price is more expensive, and the time of becoming thoroughly decomposed is long, and become thoroughly decomposed DeGrain, and peasant often seldom uses.
Summary of the invention
The object of this invention is to provide and a kind ofly prepare simple, the effects that becomes thoroughly decomposed is good straw decomposing inoculant and preparation method thereof.
The present invention is by the following technical solutions:
The invention provides a kind of straw decomposing inoculant, described straw decomposing inoculant comprises subtilis, Bacillus licheniformis, S. cervisiae, aspergillus niger and koning trichoderma.
Further, the preferred bacterial classification number ratio of described subtilis, Bacillus licheniformis, S. cervisiae, aspergillus niger and koning trichoderma is 1 ~ 3:1 ~ 3:2 ~ 6:2 ~ 4:0.5 ~ 2.
Further, the best bacterial classification number ratio of described subtilis, Bacillus licheniformis, S. cervisiae, aspergillus niger and koning trichoderma is 2:2:4:3:1.
The present invention also provides the preparation method of described straw decomposing inoculant, and the method comprises the following steps:
Subtilis, Bacillus licheniformis, S. cervisiae, aspergillus niger and koning trichoderma are cultivated respectively, being cultured to bacterium dense is 0.1 ~ 1 × 10 8/ mL, after mixing by bacterial classification number ratio, be inoculated in Caulis Sacchari sinensis leaf with the ratio of 1:10 ~ 20 and cultivate 5 ~ 10 days, culture temperature is 25 ~ 30 DEG C, and Caulis Sacchari sinensis leaf water content is 30% ~ 50%, finally Caulis Sacchari sinensis leaf is crushed to 20 ~ 100 orders i.e. obtained straw decomposing inoculant.
Beneficial effect of the present invention is:
1, bacterial classification is selected and reasonable ratio, acts synergistically between each bacterial classification, can the effective composition such as the Mierocrystalline cellulose of decomposing straw, hemicellulose and xylogen, accelerates to become thoroughly decomposed the time, within 15 days, generally gets final product effective degrading straw; 2, the preparation method of straw decomposing inoculant is simple and easy to control, and culture condition carries out under being mainly normal temperature, less investment, does not need to add expensive nutrient chemical, can reduce production cost, profit and popularization.
Embodiment
Be described with specific embodiment below, but the present invention is not limited to these embodiments.
Embodiment 1
Straw decomposing inoculant, comprises subtilis, Bacillus licheniformis, S. cervisiae, aspergillus niger and koning trichoderma.The bacterial classification number ratio of described subtilis, Bacillus licheniformis, S. cervisiae, aspergillus niger and koning trichoderma is 2:2:4:3:1.
The preparation of straw decomposing inoculant, comprises the following steps: subtilis, Bacillus licheniformis, S. cervisiae, aspergillus niger and koning trichoderma are cultivated respectively, and being cultured to bacterium dense is 0.1 × 10 8/ mL, after mixing by bacterial classification number ratio, be inoculated in Caulis Sacchari sinensis leaf with the ratio of 1:15 and cultivate 8 days, culture temperature is 28 DEG C, and Caulis Sacchari sinensis leaf water content is 40%, finally Caulis Sacchari sinensis leaf is crushed to 80 orders i.e. obtained straw decomposing inoculant.
Embodiment 2
Straw decomposing inoculant, comprises subtilis, Bacillus licheniformis, S. cervisiae, aspergillus niger and koning trichoderma.The bacterial classification number ratio of described subtilis, Bacillus licheniformis, S. cervisiae, aspergillus niger and koning trichoderma is 1:1.5:6:2:1.5.
The preparation of straw decomposing inoculant, comprises the following steps: subtilis, Bacillus licheniformis, S. cervisiae, aspergillus niger and koning trichoderma are cultivated respectively, and being cultured to bacterium dense is 1 × 10 8/ mL, after mixing by bacterial classification number ratio, be inoculated in Caulis Sacchari sinensis leaf with the ratio of 1:10 and cultivate 8 days, culture temperature is 30 DEG C, and Caulis Sacchari sinensis leaf water content is 30%, finally Caulis Sacchari sinensis leaf is crushed to 60 orders i.e. obtained straw decomposing inoculant.
Embodiment 3
Straw decomposing inoculant, comprises subtilis, Bacillus licheniformis, S. cervisiae, aspergillus niger and koning trichoderma.The bacterial classification number ratio of described subtilis, Bacillus licheniformis, S. cervisiae, aspergillus niger and koning trichoderma is 2.5:3:2:3:2.
The preparation of straw decomposing inoculant, comprises the following steps: subtilis, Bacillus licheniformis, S. cervisiae, aspergillus niger and koning trichoderma are cultivated respectively, and being cultured to bacterium dense is 0.1 × 10 8/ mL, after mixing by bacterial classification number ratio, be inoculated in Caulis Sacchari sinensis leaf with the ratio of 1:15 and cultivate 10 days, culture temperature is 25 DEG C, and Caulis Sacchari sinensis leaf water content is 35%, finally Caulis Sacchari sinensis leaf is crushed to 100 orders i.e. obtained straw decomposing inoculant.
Embodiment 4
Straw decomposing inoculant, comprises subtilis, Bacillus licheniformis, S. cervisiae, aspergillus niger and koning trichoderma.The bacterial classification number ratio of described subtilis, Bacillus licheniformis, S. cervisiae, aspergillus niger and koning trichoderma is 3:1:4:4:0.5.
The preparation of straw decomposing inoculant, comprises the following steps: subtilis, Bacillus licheniformis, S. cervisiae, aspergillus niger and koning trichoderma are cultivated respectively, and being cultured to bacterium dense is 0.5 × 10 8/ mL, after mixing by bacterial classification number ratio, be inoculated in Caulis Sacchari sinensis leaf with the ratio of 1:20 and cultivate 5 days, culture temperature is 26 DEG C, and Caulis Sacchari sinensis leaf water content is 50%, finally Caulis Sacchari sinensis leaf is crushed to 20 orders i.e. obtained straw decomposing inoculant.

Claims (4)

1. a straw decomposing inoculant, is characterized in that: described straw decomposing inoculant comprises subtilis, Bacillus licheniformis, S. cervisiae, aspergillus niger and koning trichoderma.
2. straw decomposing inoculant according to claim 1, is characterized in that: the bacterial classification number ratio of described subtilis, Bacillus licheniformis, S. cervisiae, aspergillus niger and koning trichoderma is 1 ~ 3:1 ~ 3:2 ~ 6:2 ~ 4:0.5 ~ 2.
3. straw decomposing inoculant according to claim 1, is characterized in that: the bacterial classification number ratio of described subtilis, Bacillus licheniformis, S. cervisiae, aspergillus niger and koning trichoderma is 2:2:4:3:1.
4. the preparation method of straw decomposing inoculant described in claim 1,2 or 3, it is characterized in that, the method comprises the following steps: subtilis, Bacillus licheniformis, S. cervisiae, aspergillus niger and koning trichoderma are cultivated respectively, and being cultured to bacterium dense is 0.1 ~ 1 × 10 8/ mL, after mixing by bacterial classification number ratio, be inoculated in Caulis Sacchari sinensis leaf with the ratio of 1:10 ~ 20 and cultivate 5 ~ 10 days, culture temperature is 25 ~ 30 DEG C, and Caulis Sacchari sinensis leaf water content is 30% ~ 50%, finally Caulis Sacchari sinensis leaf is crushed to 20 ~ 100 orders i.e. obtained straw decomposing inoculant.
CN201410712741.2A 2014-11-28 2014-11-28 Straw decomposing agent and preparation method thereof Pending CN104403971A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201410712741.2A CN104403971A (en) 2014-11-28 2014-11-28 Straw decomposing agent and preparation method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201410712741.2A CN104403971A (en) 2014-11-28 2014-11-28 Straw decomposing agent and preparation method thereof

Publications (1)

Publication Number Publication Date
CN104403971A true CN104403971A (en) 2015-03-11

Family

ID=52641641

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201410712741.2A Pending CN104403971A (en) 2014-11-28 2014-11-28 Straw decomposing agent and preparation method thereof

Country Status (1)

Country Link
CN (1) CN104403971A (en)

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104927873A (en) * 2015-06-08 2015-09-23 耿跃 Seedlings field soil conditioner and preparation method thereof
CN105018445A (en) * 2015-08-05 2015-11-04 吉林市新陆生态农业科技有限公司 Cellulase preparation for composite biological fungus fertilizer and preparation method thereof
CN106479912A (en) * 2016-09-08 2017-03-08 江南大学 The bacillus licheniformis of one plant of cellulase-producing and its application
WO2017138824A1 (en) * 2016-02-10 2017-08-17 Intermag Sp. Z O.O. A microbiological preparation for the mineralization of cellulose -containing organic matter and the application of the microbiological preparation in growing plants and/or in animal nutrition
CN108997034A (en) * 2018-08-10 2018-12-14 成都惠森生物技术有限公司 Decomposed starting agent of one kind and preparation method thereof
CN113748947A (en) * 2021-09-30 2021-12-07 广西壮族自治区农业科学院 Method for promoting root growth of perennial sugarcane after mechanical harvesting
CN114426438A (en) * 2022-01-04 2022-05-03 春华秋实科技集团有限公司 Organic material decomposing inoculant containing compound microorganisms and application thereof
CN115109728A (en) * 2022-07-15 2022-09-27 山东鼎创生物科技有限公司 Straw in-situ decomposition compound microbial agent and preparation method and application thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101988040A (en) * 2009-08-06 2011-03-23 南开大学 Compound bactericide for efficient degradation of landscaping garbage and preparation method thereof
CN102531766A (en) * 2011-09-15 2012-07-04 北京世纪阿姆斯生物技术股份有限公司 Microbial decomposing agent and production method thereof
CN104030746A (en) * 2014-06-24 2014-09-10 湖南豫园生物科技有限公司 Organic material decomposition agent, biological organic fertilizer containing tobacco wastes, and production method and application method of biological organic fertilizer

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101988040A (en) * 2009-08-06 2011-03-23 南开大学 Compound bactericide for efficient degradation of landscaping garbage and preparation method thereof
CN102531766A (en) * 2011-09-15 2012-07-04 北京世纪阿姆斯生物技术股份有限公司 Microbial decomposing agent and production method thereof
CN104030746A (en) * 2014-06-24 2014-09-10 湖南豫园生物科技有限公司 Organic material decomposition agent, biological organic fertilizer containing tobacco wastes, and production method and application method of biological organic fertilizer

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104927873A (en) * 2015-06-08 2015-09-23 耿跃 Seedlings field soil conditioner and preparation method thereof
CN105018445A (en) * 2015-08-05 2015-11-04 吉林市新陆生态农业科技有限公司 Cellulase preparation for composite biological fungus fertilizer and preparation method thereof
WO2017138824A1 (en) * 2016-02-10 2017-08-17 Intermag Sp. Z O.O. A microbiological preparation for the mineralization of cellulose -containing organic matter and the application of the microbiological preparation in growing plants and/or in animal nutrition
CN106479912A (en) * 2016-09-08 2017-03-08 江南大学 The bacillus licheniformis of one plant of cellulase-producing and its application
CN106479912B (en) * 2016-09-08 2019-10-15 江南大学 The bacillus licheniformis of one plant of cellulase-producing and its application
CN108997034A (en) * 2018-08-10 2018-12-14 成都惠森生物技术有限公司 Decomposed starting agent of one kind and preparation method thereof
CN113748947A (en) * 2021-09-30 2021-12-07 广西壮族自治区农业科学院 Method for promoting root growth of perennial sugarcane after mechanical harvesting
CN113748947B (en) * 2021-09-30 2022-07-01 广西壮族自治区农业科学院 Method for promoting root growth of perennial sugarcane after mechanical harvesting
CN114426438A (en) * 2022-01-04 2022-05-03 春华秋实科技集团有限公司 Organic material decomposing inoculant containing compound microorganisms and application thereof
CN115109728A (en) * 2022-07-15 2022-09-27 山东鼎创生物科技有限公司 Straw in-situ decomposition compound microbial agent and preparation method and application thereof
CN115109728B (en) * 2022-07-15 2024-05-03 山东鼎创生物科技有限公司 Straw in-situ decomposition compound microbial agent and preparation method and application thereof

Similar Documents

Publication Publication Date Title
CN104403971A (en) Straw decomposing agent and preparation method thereof
CN103031254B (en) Efficient straw-decomposing inoculant and preparation method thereof
CN104446813A (en) Bioorganic fertilizer and preparation method thereof
CN103710282B (en) Straw decomposing agent for quick start of decomposition at low temperature and its preparation method
CN102276301B (en) Straw decomposing agent and production method thereof
CN103708859B (en) Organic fertilizer and preparation method thereof
WO2015113474A1 (en) Energy grass comprehensive recycling method
CN104357362A (en) Complex microbial agent for fast decaying straws to produce organic fertilizer as well as preparation method and application
CN103614321A (en) Biological straw decomposition agent and preparation method thereof
CN104692866A (en) Biological organic fertilizer
CN104671851A (en) Corn stalk microbial fermentation organic fertilizer
CN102174400A (en) Microbial degrading agent for decomposing cassava stalks and preparation method
CN104692844A (en) Preparation method of bioorganic fertilizer
CN105152707A (en) Fermentation raw materials of vegetable seedling substrate and rapid cultivating technology of vegetable seedling substrate
CN104692961A (en) Microorganism organic material decomposition agent as well as preparation method and applications thereof
CN104045435A (en) Organic biological fertilizer for pomegranate trees
CN104311231A (en) Culture base-material for cultivating ganoderma lucidum from straw and biogas slag and preparation method thereof
CN103553831B (en) A kind of agricultural biological fertilizer
CN101288359B (en) Method for stopping clover seeds dormancy in hard seeds
CN103351188B (en) Preparation method of agricultural organic fertilizer by treating pure straws as raw material
CN110981635A (en) Agricultural microbial agent with nitrogen fixation, phosphorus dissolution, potassium dissolution and cellulose dissolution functions and preparation method thereof
CN105941003A (en) Turf-free substrate and application thereof
CN108017428A (en) A kind of straw decomposition microbial inoculum and its application
CN103708911A (en) Green soil improvement fertilizer
CN103073337A (en) Method for enhancing soil fertility by prompting straw degradation with cellulase

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
WD01 Invention patent application deemed withdrawn after publication
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20150311