CN104357362A - Complex microbial agent for fast decaying straws to produce organic fertilizer as well as preparation method and application - Google Patents
Complex microbial agent for fast decaying straws to produce organic fertilizer as well as preparation method and application Download PDFInfo
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- CN104357362A CN104357362A CN201410656485.XA CN201410656485A CN104357362A CN 104357362 A CN104357362 A CN 104357362A CN 201410656485 A CN201410656485 A CN 201410656485A CN 104357362 A CN104357362 A CN 104357362A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
Abstract
The invention relates to a complex microbial agent mainly used for fast decaying straws to produce an organic fertilizer as well as a preparation method and the application. The complex microbial agent for fast decaying the straws to produce the organic fertilizer is characterized by being prepared from 15-20% of bacillus subtilis, 16-20% of aspergillus niger, 18-22% of trichoderma reesei, 10-15% of white rot fungi, 10-15% of aspergillus oryzae, 5-7% of alkalophilic bacteria, 5-8% of thermophilic cellulose decomposition bacteria and 5-10% brewer's yeast in percentage by mass, as well as an accelerant accounting for 0.01-0.03% of the total mass of the bacillus subtilis, the aspergillus niger, the trichoderma reesei, the white rot fungi, the aspergillus oryzae, the alkalophilic bacteria, the thermophilic cellulose decomposition bacteria and the brewer's yeast. The complex microbial agent is capable of shorting the straw decaying period; the decayed straw is combined with pig manure to produce the organic fertilizer, and therefore, the straws can be utilized effectively and practically.
Description
Technical field
The present invention relates to a kind of composite fungus agent and the Synthesis and applications that are mainly used in the rotten fertilizer processed of stalk speed.
Background technology
Since reform and opening-up, the industry of China obtains develop rapidly, and traditional agriculture also achieves very much progress in industrial development process.But still there is sizable problem in modern agriculture not mature enough at present.The such as use of a large amount of agricultural chemicals causes the deterioration of ecotope, a large amount of farm crop organic matter is not utilized effectively.In recent years, along with air quality problem becomes increasingly conspicuous, the burning of stalk, as large source of pollution, becomes the hot issue that people pay close attention to gradually.China produces various stalk (wheat, paddy rice, corn etc.) about 1,300,000,000 tons every year, be wherein utilized effectively less than 20%(producing methane, direct returning to farmland etc.).And the developed countries such as Japan, Holland, the U.S., utilize the technology such as straw hydrogen, alcohol, building materials comparatively ripe, the stalk resource more than 70% obtains effective utilization.How to shorten the stalk corruption cycle, select the problems such as more practical application approach to become the bottleneck of Related Research Domain.
The main component of stalk is Mierocrystalline cellulose, hemicellulose and xylogen etc., and its surface is also covered with the wax layer of one deck densification.The parcel of the integrated structure of natural polymer wax layer in addition, make the decomposition rate that becomes thoroughly decomposed of stalk under field conditions (factors) very slow, the cycle is very long, is approximately one and a half months.The bacterium of the effect enzymes such as eccrine fiber element lytic enzyme, hemicellulose lytic enzyme, lignin decomposition enzyme, under natural condition, functioning efficiency is lower, and reason is the restriction of the factors such as nutrient, temperature, humidity, pH.Lignin degradation is the main bugbear that straw-like materials utilizes, its degradation method is also a lot, physics, chemistry, biology have, but there is secondary pollution problem in total end chemical method, Physical efficiency is lower, grinding refinement or the cost of explosion higher, although and biological process receives heat at present and dotes on, inreal application, the factors such as the cycle that is mainly subject to processing is longer, actual effect is not good limit.
In conjunction with the research previously about project research, use for reference test (the Anhui agronomy circular of the people such as Wang's root method, Anhui.Agri.Sci.Bull.2012,18 (01)) enlighten, probing into through lot of experiments, invent a kind of composite fungus agent being applied to High-efficiency straw corruption, and it is combined with pig manure has made fertilizer, obtain comparatively ideal effect.
Summary of the invention
The object of the present invention is to provide a kind of composite fungus agent and the Synthesis and applications that are applied to the rotten fertilizer processed of stalk speed, this composite fungus agent can shorten stalk abrasion cycle, is combined makes fertilizer after becoming thoroughly decomposed with pig manure, can realize effectively utilizing the reality of stalk.
For achieving the above object, the technical solution used in the present invention is: a kind of composite fungus agent being applied to the rotten fertilizer processed of stalk speed, it is characterized in that it is made up of subtilis, aspergillus niger, Trichodermareesei, whiterot fungi, aspergillus oryzae, alkaliphile, thermophilic cellulose-decomposing bacterium, yeast saccharomyces cerevisiae, promotor, the mass percent shared by various bacterium is: subtilis 15-20%, aspergillus niger 16-20%, Trichodermareesei 18-22%, whiterot fungi 10-15%, aspergillus oryzae 10-15%, alkaliphile 5-7%, thermophilic cellulose-decomposing bacterium 5-8%, yeast saccharomyces cerevisiae 5-10%; The consumption of promotor is the 0.01-0.03% of subtilis, aspergillus niger, Trichodermareesei, whiterot fungi, aspergillus oryzae, alkaliphile, thermophilic cellulose-decomposing bacterium and yeast saccharomyces cerevisiae total mass.
Described subtilis effective bacterium number accounts for total effectively hundreds of proportion by subtraction 15-20% of bacterium.
Described aspergillus niger effective bacterium number accounts for total effectively hundreds of proportion by subtraction 16-20% of bacterium.
Described Trichodermareesei effective bacterium number accounts for total effectively hundreds of proportion by subtraction 18-22% of bacterium.
Described whiterot fungi effective bacterium number accounts for total effectively hundreds of proportion by subtraction 10-15% of bacterium.
Described aspergillus oryzae effective bacterium number accounts for total effectively hundreds of proportion by subtraction 10-15% of bacterium.
Described alkaliphile effective bacterium number accounts for total effectively hundreds of proportion by subtraction 5-7% of bacterium.
Described thermophilic cellulose-decomposing bacterium effective bacterium number accounts for total effectively hundreds of proportion by subtraction 5-8% of bacterium.
Described yeast saccharomyces cerevisiae effective bacterium number accounts for total effectively hundreds of proportion by subtraction 5-10% of bacterium.
By technique scheme, best, the mass percent shared by various bacterium is: subtilis 17%, aspergillus niger 20%, Trichodermareesei 20%, whiterot fungi 10%, aspergillus oryzae 13%, alkaliphile 5%, thermophilic cellulose-decomposing bacterium 7%, yeast saccharomyces cerevisiae 8%.
By technique scheme, best, described subtilis effective bacterium number accounts for total effectively hundreds of proportion by subtraction 17% of bacterium.Described aspergillus niger effective bacterium number accounts for total effectively hundreds of proportion by subtraction 20% of bacterium.Described Trichodermareesei effective bacterium number accounts for total effectively hundreds of proportion by subtraction 20% of bacterium.Described whiterot fungi effective bacterium number accounts for total effectively hundreds of proportion by subtraction 10% of bacterium.Described aspergillus oryzae effective bacterium number accounts for total effectively hundreds of proportion by subtraction 13% of bacterium.Described alkaliphile effective bacterium number accounts for total effectively hundreds of proportion by subtraction 5% of bacterium.Described thermophilic cellulose-decomposing bacterium effective bacterium number accounts for total effectively hundreds of proportion by subtraction 7% of bacterium.Described yeast saccharomyces cerevisiae effective bacterium number accounts for total effectively hundreds of proportion by subtraction 8% of bacterium.
Described promotor is polyamines promotor.
Above-mentioned a kind of preparation method being applied to the composite fungus agent of the rotten fertilizer processed of stalk speed, is characterized in that it comprises the steps:
1) by the mass percent shared by various bacterium be: subtilis 15-20%, aspergillus niger 16-20%, Trichodermareesei 18-22%, whiterot fungi 10-15%, aspergillus oryzae 10-15%, alkaliphile 5-7%, thermophilic cellulose-decomposing bacterium 5-8%, yeast saccharomyces cerevisiae 5-10%; The consumption of promotor is the 0.01-0.03% of subtilis, aspergillus niger, Trichodermareesei, whiterot fungi, aspergillus oryzae, alkaliphile, thermophilic cellulose-decomposing bacterium and yeast saccharomyces cerevisiae total mass;
Choose subtilis, aspergillus niger, Trichodermareesei, whiterot fungi, aspergillus oryzae, alkaliphile, thermophilic cellulose-decomposing bacterium, yeast saccharomyces cerevisiae, promotor, for subsequent use;
2) by the consumption of microbial culture medium be the 0.01-0.03% of subtilis, aspergillus niger, Trichodermareesei, whiterot fungi, aspergillus oryzae, alkaliphile, thermophilic cellulose-decomposing bacterium, yeast saccharomyces cerevisiae and promotor total mass, choose microbial culture medium; By subtilis, aspergillus niger, Trichodermareesei, whiterot fungi, aspergillus oryzae, alkaliphile, thermophilic cellulose-decomposing bacterium, yeast saccharomyces cerevisiae and promotor mixing, cultivate with microbial culture medium, first cultivate 30min, then after stirring, dry 30min, obtained composite fungus agent (powder).
Described microbial culture medium is: beef-protein medium (formula extractum carnis 3.0g, peptone 10.0g, NaCl 5. g, agar 15-25g, water 1000ml, pH 7.4-7.6).
Above-mentioned a kind of application being applied to the composite fungus agent of the rotten fertilizer processed of stalk speed, is characterized in that it comprises the steps:
1), by agricultural crop straw pulverizer be crushed to 0.3-0.5cm diameter particle, obtain the stalk after pulverizing;
2), according to composite fungus agent: the mass ratio=1:1500 of the stalk after pulverizing, according to composite fungus agent: the mass ratio=1:25 of water, water-soluble composite fungus agent and the stalk after pulverizing fully are mixed evenly, obtain the stalk after composite fungus agent process;
3), according to the stalk after composite fungus agent process: the mass ratio=1:4 of the pig manure of intermediate moist, the pig manure (water ratio is 60-70 quality %) of intermediate moist is mixed with the stalk after above-mentioned composite fungus agent process;
4), fermentation 9-11 days (mixed biological fertilizer is black gray expandable, and stalk is integrated with pig manure elementary mixing);
5), carry out air-dry granulation, pack, sealing (directly open bag during use, use by every mu of 40kg), obtains pure Environmental-friendbiological biological organic fertilizer.
The present invention's main bacteria seed used is as follows:
Subtilis: the enzymes such as subtilis thalline self synthesis α-amylase, proteolytic enzyme, lipase, cellulase.
Aspergillus niger: industrial at bio-feritlizer, aspergillus niger has cracking larger molecular organics and indissoluble inorganics, is convenient to Crop and utilizes, improve Soil structure, strengthen soil fertility, improve the effect of crop yield.
Trichodermareesei: it for the production of the enzyme decomposing different vegetable material, comprises cellulase, hemicellulase, proteolytic enzyme, amylase etc. as industrial strain, existing history for many years.A kind of main cellulase one cellobiohydrolase I(cellobiohydrolase1 that Trichodermareesei produces).
Aspergillus oryzae: be the bacterial strain that a class produces prozyme, except product proteolytic enzyme, also can produce amylase, saccharifying enzyme, cellulase, phytase etc.
Whiterot fungi: utilize whiterot fungi can lignin degrading, hemicellulose and cellulosic characteristic.
All the other auxiliary bacteria comprise: alkaliphile, thermophilic cellulose-decomposing bacterium, yeast saccharomyces cerevisiae, its role is to create be conducive to the condition such as stalk and the temperature needed for other main bacteria seed, pH, oxygen.Also comprise promotor, affect eubolism, promote the accumulation of mesostate, improve the output of secondary metabolite.
The invention has the beneficial effects as follows:
1) composite fungus agent of the present invention has efficient-decomposition effect to the main component such as Mierocrystalline cellulose, hemicellulose, xylogen, crude protein in the nonirrigated farmlands such as wheat, paddy rice, corn, paddy field farming stalk, said components substantially can be decomposed and finish in 10 days; Shorten stalk abrasion cycle.
2) with the stalk after composite fungus agent process, then mix with pig manure according to the ratio of 1:4, make biological organic fertilizer also field by granulator granulation; Composite fungus agent makes stalk be utilized effectively as the biological organic resource of one, effectively can suppress vast Rural areas crop straw burning phenomenon simultaneously, pollution abatement protection air; By the agricultural crop straw after this composite fungus agent process, the fertilizer that mixing pig manure is made has higher fertilizer efficiency, and saved agricultural cost, this fertilizer avoids the hardening soil and the loss of moisture simultaneously; The fertilizer made takes from bacteria agent and natural manure, to environment and farm crop toxicological harmless effect.This composite fungus agent and the application thereof being applied to efficient straw quick rotting agent, has economy and feature of environmental protection double benefit, and integrated application is worth higher, achieves and effectively utilizes the reality of stalk.
3) a kind of application processing mode is newly provided, bacteria agent is utilized to shorten stalk abrasion cycle on the one hand as far as possible, add pig manure by a certain percentage on the other hand, compensate for stalk short-term can not the problem such as burn into fertilizer efficiency is not good completely, the two is made pure Environmental-friendbiological biological organic fertilizer, achieves and the reality of stalk is effectively utilized.
Embodiment
Below in conjunction with specific embodiment, a kind of composite fungus agent effect being applied to the rotten fertilizer processed of stalk speed of the present invention is described further, but the scope that the present invention protects is not limited to the scope of embodiment.
The medicine used in following embodiment is commercially available prod or laboratory conventional medication, and bacterial classification is the bacterial classification of above-mentioned explanation.
In following embodiment: a kind of application being applied to the composite fungus agent of the rotten fertilizer processed of stalk speed, it comprises the steps:
1), by agricultural crop straw pulverizer be crushed to 0.3-0.5cm diameter particle, obtain the stalk after pulverizing;
2), according to composite fungus agent: the mass ratio=1:1500 of the stalk after pulverizing, according to composite fungus agent: the mass ratio=1:25 of water, water-soluble composite fungus agent and the stalk after pulverizing fully are mixed evenly, obtain the stalk after composite fungus agent process;
3), according to the stalk after composite fungus agent process: the mass ratio=1:4 of the pig manure of intermediate moist, the pig manure (water ratio is 60-70 quality %) of intermediate moist is mixed with the stalk after above-mentioned composite fungus agent process;
4), fermentation 9-11 days (mixed biological fertilizer is black gray expandable, and stalk is integrated with pig manure elementary mixing);
5), carry out air-dry granulation, pack, sealing (directly open bag during use, use by every mu of 40kg), obtains pure Environmental-friendbiological biological organic fertilizer.
embodiment 1
A kind of composite fungus agent being applied to the rotten fertilizer processed of stalk speed, it is made up of subtilis, aspergillus niger, Trichodermareesei, whiterot fungi, aspergillus oryzae, alkaliphile, thermophilic cellulose-decomposing bacterium, yeast saccharomyces cerevisiae, promotor, and the mass percent shared by various bacterium is: subtilis 15%, aspergillus niger 18%, Trichodermareesei 20%, whiterot fungi 12%, aspergillus oryzae 15%, alkaliphile 5%, thermophilic cellulose-decomposing bacterium 7%, yeast saccharomyces cerevisiae 8%; The consumption of promotor is the 0.01-0.03% of subtilis, aspergillus niger, Trichodermareesei, whiterot fungi, aspergillus oryzae, alkaliphile, thermophilic cellulose-decomposing bacterium and yeast saccharomyces cerevisiae total mass.
Described promotor is polyamines promotor [Zhengzhou chemical industry Food Co., Ltd free from worldly cares, L-Orn (Ornithine)].
Above-mentioned a kind of preparation method being applied to the composite fungus agent of the rotten fertilizer processed of stalk speed, it comprises the steps:
1) various bacterium and promotor is chosen by said ratio, for subsequent use;
2) by the consumption of microbial culture medium be the 0.01-0.03% of subtilis, aspergillus niger, Trichodermareesei, whiterot fungi, aspergillus oryzae, alkaliphile, thermophilic cellulose-decomposing bacterium, yeast saccharomyces cerevisiae and promotor total mass, choose microbial culture medium; By subtilis, aspergillus niger, Trichodermareesei, whiterot fungi, aspergillus oryzae, alkaliphile, thermophilic cellulose-decomposing bacterium, yeast saccharomyces cerevisiae and promotor mixing, cultivate with microbial culture medium, first cultivate 30min, then after stirring, dry 30min, obtained composite fungus agent (powder).
Described microbial culture medium is: beef-protein medium (formula extractum carnis 3.0g, peptone 10.0g, NaCl 5. g, agar 15-25g, water 1000ml, pH 7.4-7.6).
Above-mentioned a kind of application (i.e. using method) being applied to the composite fungus agent of the rotten fertilizer processed of stalk speed, with above-mentioned.
embodiment 2
A kind of composite fungus agent being applied to the rotten fertilizer processed of stalk speed, it is made up of subtilis, aspergillus niger, Trichodermareesei, whiterot fungi, aspergillus oryzae, alkaliphile, thermophilic cellulose-decomposing bacterium, yeast saccharomyces cerevisiae, promotor, and the mass percent shared by various bacterium is: subtilis 15%, aspergillus niger 20%, Trichodermareesei 20%, whiterot fungi 12%, aspergillus oryzae 13%, alkaliphile 5%, thermophilic cellulose-decomposing bacterium 7%, yeast saccharomyces cerevisiae 8%; The consumption of promotor be subtilis, aspergillus niger, Trichodermareesei, whiterot fungi, aspergillus oryzae, alkaliphile, thermophilic cellulose-decomposing bacterium and yeast saccharomyces cerevisiae total mass 0.02%.
Described promotor is polyamines promotor [Zhengzhou chemical industry Food Co., Ltd free from worldly cares, L-Orn (Ornithine)].
Above-mentioned a kind of preparation method being applied to the composite fungus agent of the rotten fertilizer processed of stalk speed, with embodiment 1.
Above-mentioned a kind of application (using method) being applied to the composite fungus agent of the rotten fertilizer processed of stalk speed, with above-mentioned.
embodiment 3
A kind of composite fungus agent being applied to the rotten fertilizer processed of stalk speed, it is made up of subtilis, aspergillus niger, Trichodermareesei, whiterot fungi, aspergillus oryzae, alkaliphile, thermophilic cellulose-decomposing bacterium, yeast saccharomyces cerevisiae, promotor, and the mass percent shared by various bacterium is: subtilis 15%, aspergillus niger 20%, Trichodermareesei 20%, whiterot fungi 10%, aspergillus oryzae 15%, alkaliphile 5%, thermophilic cellulose-decomposing bacterium 7%, yeast saccharomyces cerevisiae 8%; The consumption of promotor be subtilis, aspergillus niger, Trichodermareesei, whiterot fungi, aspergillus oryzae, alkaliphile, thermophilic cellulose-decomposing bacterium and yeast saccharomyces cerevisiae total mass 0.02%.
Described promotor is polyamines promotor [Zhengzhou chemical industry Food Co., Ltd free from worldly cares, L-Orn (Ornithine)].
Above-mentioned a kind of preparation method being applied to the composite fungus agent of the rotten fertilizer processed of stalk speed, with embodiment 1.
Above-mentioned a kind of application (using method) being applied to the composite fungus agent of the rotten fertilizer processed of stalk speed, with above-mentioned.
embodiment 4
A kind of composite fungus agent being applied to the rotten fertilizer processed of stalk speed, it is made up of subtilis, aspergillus niger, Trichodermareesei, whiterot fungi, aspergillus oryzae, alkaliphile, thermophilic cellulose-decomposing bacterium, yeast saccharomyces cerevisiae, promotor, and the mass percent shared by various bacterium is: subtilis 15%, aspergillus niger 20%, Trichodermareesei 18%, whiterot fungi 12%, aspergillus oryzae 15%, alkaliphile 5%, thermophilic cellulose-decomposing bacterium 7%, yeast saccharomyces cerevisiae 8%; The consumption of promotor be subtilis, aspergillus niger, Trichodermareesei, whiterot fungi, aspergillus oryzae, alkaliphile, thermophilic cellulose-decomposing bacterium and yeast saccharomyces cerevisiae total mass 0.02%.
Described promotor is polyamines promotor [Zhengzhou chemical industry Food Co., Ltd free from worldly cares, L-Orn (Ornithine)].
Above-mentioned a kind of preparation method being applied to the composite fungus agent of the rotten fertilizer processed of stalk speed, with embodiment 1.
Above-mentioned a kind of application (using method) being applied to the composite fungus agent of the rotten fertilizer processed of stalk speed, with above-mentioned.
embodiment 5
A kind of composite fungus agent being applied to the rotten fertilizer processed of stalk speed, it is made up of subtilis, aspergillus niger, Trichodermareesei, whiterot fungi, aspergillus oryzae, alkaliphile, thermophilic cellulose-decomposing bacterium, yeast saccharomyces cerevisiae, promotor, and the mass percent shared by various bacterium is: subtilis 17%, aspergillus niger 18%, Trichodermareesei 18%, whiterot fungi 12%, aspergillus oryzae 15%, alkaliphile 5%, thermophilic cellulose-decomposing bacterium 7%, yeast saccharomyces cerevisiae 8%; The consumption of promotor is the 0.01-0.03% of subtilis, aspergillus niger, Trichodermareesei, whiterot fungi, aspergillus oryzae, alkaliphile, thermophilic cellulose-decomposing bacterium and yeast saccharomyces cerevisiae total mass.
Described promotor is polyamines promotor [Zhengzhou chemical industry Food Co., Ltd free from worldly cares, L-Orn (Ornithine)].
Above-mentioned a kind of preparation method being applied to the composite fungus agent of the rotten fertilizer processed of stalk speed, with embodiment 1.
Above-mentioned a kind of application (using method) being applied to the composite fungus agent of the rotten fertilizer processed of stalk speed, with above-mentioned.
embodiment 6
A kind of composite fungus agent being applied to the rotten fertilizer processed of stalk speed, it is made up of subtilis, aspergillus niger, Trichodermareesei, whiterot fungi, aspergillus oryzae, alkaliphile, thermophilic cellulose-decomposing bacterium, yeast saccharomyces cerevisiae, promotor, and the mass percent shared by various bacterium is: subtilis 17%, aspergillus niger 20%, Trichodermareesei 18%, whiterot fungi 10%, aspergillus oryzae 15%, alkaliphile 5%, thermophilic cellulose-decomposing bacterium 7%, yeast saccharomyces cerevisiae 8%; The consumption of promotor is the 0.01-0.03% of subtilis, aspergillus niger, Trichodermareesei, whiterot fungi, aspergillus oryzae, alkaliphile, thermophilic cellulose-decomposing bacterium and yeast saccharomyces cerevisiae total mass.
Described promotor is polyamines promotor [Zhengzhou chemical industry Food Co., Ltd free from worldly cares, L-Orn (Ornithine)].
Above-mentioned a kind of preparation method being applied to the composite fungus agent of the rotten fertilizer processed of stalk speed, with embodiment 1.
Above-mentioned a kind of application (using method) being applied to the composite fungus agent of the rotten fertilizer processed of stalk speed, with above-mentioned.
embodiment 7
A kind of composite fungus agent being applied to the rotten fertilizer processed of stalk speed, it is made up of subtilis, aspergillus niger, Trichodermareesei, whiterot fungi, aspergillus oryzae, alkaliphile, thermophilic cellulose-decomposing bacterium, yeast saccharomyces cerevisiae, promotor, and the mass percent shared by various bacterium is: subtilis 17%, aspergillus niger 20%, Trichodermareesei 20%, whiterot fungi 10%, aspergillus oryzae 13%, alkaliphile 5%, thermophilic cellulose-decomposing bacterium 7%, yeast saccharomyces cerevisiae 8%; The consumption of promotor is the 0.01-0.03% of subtilis, aspergillus niger, Trichodermareesei, whiterot fungi, aspergillus oryzae, alkaliphile, thermophilic cellulose-decomposing bacterium and yeast saccharomyces cerevisiae total mass.
Described promotor is polyamines promotor [Zhengzhou chemical industry Food Co., Ltd free from worldly cares, L-Orn (Ornithine)].
Above-mentioned a kind of preparation method being applied to the composite fungus agent of the rotten fertilizer processed of stalk speed, with embodiment 1.
Above-mentioned a kind of application (using method) being applied to the composite fungus agent of the rotten fertilizer processed of stalk speed, with above-mentioned.
embodiment 8
A kind of composite fungus agent being applied to the rotten fertilizer processed of stalk speed, it is made up of subtilis, aspergillus niger, Trichodermareesei, whiterot fungi, aspergillus oryzae, alkaliphile, thermophilic cellulose-decomposing bacterium, yeast saccharomyces cerevisiae, promotor, and the mass percent shared by various bacterium is: subtilis 17%, aspergillus niger 18%, Trichodermareesei 20%, whiterot fungi 10%, aspergillus oryzae 15%, alkaliphile 5%, thermophilic cellulose-decomposing bacterium 7%, yeast saccharomyces cerevisiae 8%; The consumption of promotor is the 0.01-0.03% of subtilis, aspergillus niger, Trichodermareesei, whiterot fungi, aspergillus oryzae, alkaliphile, thermophilic cellulose-decomposing bacterium and yeast saccharomyces cerevisiae total mass.
Described promotor is polyamines promotor [Zhengzhou chemical industry Food Co., Ltd free from worldly cares, L-Orn (Ornithine)].
Above-mentioned a kind of preparation method being applied to the composite fungus agent of the rotten fertilizer processed of stalk speed, with embodiment 1.
Above-mentioned a kind of application (using method) being applied to the composite fungus agent of the rotten fertilizer processed of stalk speed, with above-mentioned.
embodiment 9
A kind of composite fungus agent being applied to the rotten fertilizer processed of stalk speed, it is made up of subtilis, aspergillus niger, Trichodermareesei, whiterot fungi, aspergillus oryzae, alkaliphile, thermophilic cellulose-decomposing bacterium, yeast saccharomyces cerevisiae, promotor, and the mass percent shared by various bacterium is: subtilis 17%, aspergillus niger 18%, Trichodermareesei 20%, whiterot fungi 12%, aspergillus oryzae 13%, alkaliphile 5%, thermophilic cellulose-decomposing bacterium 7%, yeast saccharomyces cerevisiae 8%; The consumption of promotor is the 0.01-0.03% of subtilis, aspergillus niger, Trichodermareesei, whiterot fungi, aspergillus oryzae, alkaliphile, thermophilic cellulose-decomposing bacterium and yeast saccharomyces cerevisiae total mass.
Described promotor is polyamines promotor [Zhengzhou chemical industry Food Co., Ltd free from worldly cares, L-Orn (Ornithine)].
Above-mentioned a kind of preparation method being applied to the composite fungus agent of the rotten fertilizer processed of stalk speed, with embodiment 1.
Above-mentioned a kind of application (using method) being applied to the composite fungus agent of the rotten fertilizer processed of stalk speed, with above-mentioned.
embodiment 10(comparative example does not add promotor)
A kind of composite fungus agent being applied to the rotten fertilizer processed of stalk speed, it is made up of subtilis, aspergillus niger, Trichodermareesei, whiterot fungi, aspergillus oryzae, alkaliphile, thermophilic cellulose-decomposing bacterium, yeast saccharomyces cerevisiae, promotor, and the mass percent shared by various bacterium is: subtilis 15%, aspergillus niger 18%, Trichodermareesei 20%, whiterot fungi 12%, aspergillus oryzae 15%, alkaliphile 5%, thermophilic cellulose-decomposing bacterium 7%, yeast saccharomyces cerevisiae 8%; The consumption of promotor is the 0.01-0.03% of subtilis, aspergillus niger, Trichodermareesei, whiterot fungi, aspergillus oryzae, alkaliphile, thermophilic cellulose-decomposing bacterium and yeast saccharomyces cerevisiae total mass.
Described promotor is polyamines promotor [Zhengzhou chemical industry Food Co., Ltd free from worldly cares, L-Orn (Ornithine)].
Above-mentioned a kind of preparation method being applied to the composite fungus agent of the rotten fertilizer processed of stalk speed, with embodiment 1(without promotor).
Above-mentioned a kind of application (using method) being applied to the composite fungus agent of the rotten fertilizer processed of stalk speed, with above-mentioned.
embodiment 11
A kind of composite fungus agent being applied to the rotten fertilizer processed of stalk speed, it is made up of subtilis, aspergillus niger, Trichodermareesei, whiterot fungi, aspergillus oryzae, alkaliphile, thermophilic cellulose-decomposing bacterium, yeast saccharomyces cerevisiae, promotor, and the mass percent shared by various bacterium is: subtilis 20%, aspergillus niger 16%, Trichodermareesei 22%, whiterot fungi 15%, aspergillus oryzae 10%, alkaliphile 7%, thermophilic cellulose-decomposing bacterium 5%, yeast saccharomyces cerevisiae 5%; The consumption of promotor be subtilis, aspergillus niger, Trichodermareesei, whiterot fungi, aspergillus oryzae, alkaliphile, thermophilic cellulose-decomposing bacterium and yeast saccharomyces cerevisiae total mass 0.01%.
Described promotor is polyamines promotor [Zhengzhou chemical industry Food Co., Ltd free from worldly cares, L-Orn (Ornithine)].
Above-mentioned a kind of preparation method being applied to the composite fungus agent of the rotten fertilizer processed of stalk speed, with embodiment 1.
Above-mentioned a kind of application (using method) being applied to the composite fungus agent of the rotten fertilizer processed of stalk speed, with above-mentioned.
embodiment 12
A kind of composite fungus agent being applied to the rotten fertilizer processed of stalk speed, it is made up of subtilis, aspergillus niger, Trichodermareesei, whiterot fungi, aspergillus oryzae, alkaliphile, thermophilic cellulose-decomposing bacterium, yeast saccharomyces cerevisiae, promotor, and the mass percent shared by various bacterium is: subtilis 16%, aspergillus niger 18%, Trichodermareesei 22%, whiterot fungi 10%, aspergillus oryzae 10%, alkaliphile 6%, thermophilic cellulose-decomposing bacterium 8%, yeast saccharomyces cerevisiae 10%; The consumption of promotor be subtilis, aspergillus niger, Trichodermareesei, whiterot fungi, aspergillus oryzae, alkaliphile, thermophilic cellulose-decomposing bacterium and yeast saccharomyces cerevisiae total mass 0.03%.
Described promotor is polyamines promotor [Zhengzhou chemical industry Food Co., Ltd free from worldly cares, L-Orn (Ornithine)].
Above-mentioned a kind of preparation method being applied to the composite fungus agent of the rotten fertilizer processed of stalk speed, with embodiment 1.
Above-mentioned a kind of application (using method) being applied to the composite fungus agent of the rotten fertilizer processed of stalk speed, with above-mentioned.
Table 1 field experiment: (Hubei Province, Xiaochang County, steep mountain area)
| Embodiment | The pure Environmental-friendbiological biological organic fertilizer of the present invention, uses (mu) | Soil with organic matter content (before using) | Soil with organic matter content (after using) |
| 1 | 0.5 | 1.7% | 5.1% |
| 2 | 0.5 | 1.7% | 5.2% |
| 3 | 0.5 | 1.7% | 5.2% |
| 4 | 0.5 | 1.7% | 5.3% |
| 5 | 0.5 | 1.7% | 5.2% |
| 6 | 0.5 | 1.7% | 5.5% |
| 7 | 0.5 | 1.7% | 5.6% |
| 8 | 0.5 | 1.7% | 5.1% |
| 9 | 0.5 | 1.7% | 5.4% |
| 10 | 0.5 | 1.7% | 4.8% |
| 11 | 0.5 | 1.7% | 5.1% |
| 12 | 0.5 | 1.7% | 5.1% |
Experiment shows that composite fungus agent of the present invention can accelerate the decomposition of stalk, in conjunction with the organic content of the bio-organic fertilizer raising soil 3.1-3.9% that pig manure is made.
Claims (9)
1. one kind is applied to the composite fungus agent of the rotten fertilizer processed of stalk speed, it is characterized in that it is made up of subtilis, aspergillus niger, Trichodermareesei, whiterot fungi, aspergillus oryzae, alkaliphile, thermophilic cellulose-decomposing bacterium, yeast saccharomyces cerevisiae, promotor, the mass percent shared by various bacterium is: subtilis 15-20%, aspergillus niger 16-20%, Trichodermareesei 18-22%, whiterot fungi 10-15%, aspergillus oryzae 10-15%, alkaliphile 5-7%, thermophilic cellulose-decomposing bacterium 5-8%, yeast saccharomyces cerevisiae 5-10%; The consumption of promotor is the 0.01-0.03% of subtilis, aspergillus niger, Trichodermareesei, whiterot fungi, aspergillus oryzae, alkaliphile, thermophilic cellulose-decomposing bacterium and yeast saccharomyces cerevisiae total mass.
2. a kind of composite fungus agent being applied to the rotten fertilizer processed of stalk speed according to claim 1, is characterized in that, described subtilis effective bacterium number accounts for total effectively hundreds of proportion by subtraction 16-20% of bacterium; Described aspergillus niger effective bacterium number accounts for total effectively hundreds of proportion by subtraction 15-20% of bacterium; Described Trichodermareesei effective bacterium number accounts for total effectively hundreds of proportion by subtraction 18-22% of bacterium; Described whiterot fungi effective bacterium number accounts for total effectively hundreds of proportion by subtraction 10-15% of bacterium; Described aspergillus oryzae effective bacterium number accounts for total effectively hundreds of proportion by subtraction 10-15% of bacterium; Described alkaliphile effective bacterium number accounts for total effectively hundreds of proportion by subtraction 5-7% of bacterium; Described thermophilic cellulose-decomposing bacterium effective bacterium number accounts for total effectively hundreds of proportion by subtraction 5-8% of bacterium; Described yeast saccharomyces cerevisiae effective bacterium number accounts for total effectively hundreds of proportion by subtraction 5-10% of bacterium.
3. a kind of composite fungus agent being applied to the rotten fertilizer processed of stalk speed according to claim 1, it is characterized in that, the mass percent shared by various bacterium is: subtilis 17%, aspergillus niger 20%, Trichodermareesei 20%, whiterot fungi 10%, aspergillus oryzae 13%, alkaliphile 5%, thermophilic cellulose-decomposing bacterium 7%, yeast saccharomyces cerevisiae 8%.
4. a kind of composite fungus agent being applied to the rotten fertilizer processed of stalk speed according to claim 3, is characterized in that, described subtilis effective bacterium number accounts for total effectively hundreds of proportion by subtraction 17% of bacterium; Described aspergillus niger effective bacterium number accounts for total effectively hundreds of proportion by subtraction 20% of bacterium; Described Trichodermareesei effective bacterium number accounts for total effectively hundreds of proportion by subtraction 20% of bacterium; Described whiterot fungi effective bacterium number accounts for total effectively hundreds of proportion by subtraction 10% of bacterium; Described aspergillus oryzae effective bacterium number accounts for total effectively hundreds of proportion by subtraction 13% of bacterium; Described alkaliphile effective bacterium number accounts for total effectively hundreds of proportion by subtraction 5% of bacterium; Described thermophilic cellulose-decomposing bacterium effective bacterium number accounts for total effectively hundreds of proportion by subtraction 7% of bacterium; Described yeast saccharomyces cerevisiae effective bacterium number accounts for total effectively hundreds of proportion by subtraction 8% of bacterium.
5. a kind of composite fungus agent being applied to the rotten fertilizer processed of stalk speed according to claim 1, it is characterized in that, described promotor is polyamines promotor.
6. a kind of preparation method being applied to the composite fungus agent of the rotten fertilizer processed of stalk speed as claimed in claim 1, is characterized in that it comprises the steps:
1) by the mass percent shared by various bacterium be: subtilis 15-20%, aspergillus niger 16-20%, Trichodermareesei 18-22%, whiterot fungi 10-15%, aspergillus oryzae 10-15%, alkaliphile 5-7%, thermophilic cellulose-decomposing bacterium 5-8%, yeast saccharomyces cerevisiae 5-10%; The consumption of promotor is the 0.01-0.03% of subtilis, aspergillus niger, Trichodermareesei, whiterot fungi, aspergillus oryzae, alkaliphile, thermophilic cellulose-decomposing bacterium and yeast saccharomyces cerevisiae total mass;
Choose subtilis, aspergillus niger, Trichodermareesei, whiterot fungi, aspergillus oryzae, alkaliphile, thermophilic cellulose-decomposing bacterium, yeast saccharomyces cerevisiae, promotor, for subsequent use;
2) by the consumption of microbial culture medium be the 0.01-0.03% of subtilis, aspergillus niger, Trichodermareesei, whiterot fungi, aspergillus oryzae, alkaliphile, thermophilic cellulose-decomposing bacterium, yeast saccharomyces cerevisiae and promotor total mass, choose microbial culture medium; By subtilis, aspergillus niger, Trichodermareesei, whiterot fungi, aspergillus oryzae, alkaliphile, thermophilic cellulose-decomposing bacterium, yeast saccharomyces cerevisiae and promotor mixing, cultivate with microbial culture medium, first cultivate 30min, then after stirring, dry 30min, obtained composite fungus agent.
7. a kind of preparation method being applied to the composite fungus agent of the rotten fertilizer processed of stalk speed according to claim 6, it is characterized in that, described microbial culture medium is beef-protein medium.
8. a kind of application being applied to the composite fungus agent of the rotten fertilizer processed of stalk speed as described in claim 1 or 6, is characterized in that it comprises the steps:
1), by agricultural crop straw pulverizer be crushed to 0.3-0.5cm diameter particle, obtain the stalk after pulverizing;
2), according to composite fungus agent: the mass ratio=1:1500 of the stalk after pulverizing, according to composite fungus agent: the mass ratio=1:25 of water, water-soluble composite fungus agent and the stalk after pulverizing fully are mixed evenly, obtain the stalk after composite fungus agent process;
3), according to the stalk after composite fungus agent process: the mass ratio=1:4 of the pig manure of intermediate moist, the pig manure of intermediate moist is mixed with the stalk after above-mentioned composite fungus agent process;
4), ferment 9-11 days;
5), carry out air-dry granulation, pack, sealing, obtains pure Environmental-friendbiological biological organic fertilizer.
9. a kind of application being applied to the composite fungus agent of the rotten fertilizer processed of stalk speed according to claim 8, it is characterized in that, the water ratio of the pig manure of described intermediate moist is 60-70%.
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