KR20100137280A - Manufacturing method of lactic acid deodorant using agricultural residual products and lactic acid deodorant thereby - Google Patents

Abstract

PURPOSE: A method for preparing lactic acid deodorizing agent and a lactic acid deodorizing agent prepared thereby are provided, which can use sterilizing power and deodorization effect. CONSTITUTION: A method for preparing lactic acid deodorizing agent comprises: a lactobacillus cultivation step(S01) of cultivating lactobacillus by vaccinating lactobacillus spawn after sterilizing culture medium prepared by mixing agricultural by-product solution with yeast extract, white sugar, trisodium citrate, sodium phosphate, and purified water; an acid control step(S02) of controlling concentration of lactic acid by adding an diluted solution of lactic acid; and a finishing step(S03) sterilizing the lactic acid liquid.

Description

Manufacturing Method of Lactic Acid Deodorant Using Agricultural Residual Products And Lactic Acid Deodorant Thereby

The present invention generally relates to a lactic acid deodorant production technology, and more specifically, by using a crushed liquid of byproducts of agricultural products such as Chinese cabbage, cabbage, lettuce (hereinafter referred to as "agricultural by-product") by culturing the lactic acid bacteria deodorant The present invention relates to a method for producing a lactic acid deodorant using a new agricultural by-product, and a lactic acid deodorant, by which the production cost of the deodorant can be reduced and the processing cost of the agricultural by-product can be reduced.

The amount of agricultural by-products generated in Korea exceeds 10 million tons per year, of which 20 to 30% is discharged as waste. Most of these agricultural by-products are treated by landfill or incineration. For example, the amount of agricultural by-products generated daily in the Garak-dong agricultural and fishery market is about 360 tons, and the cost of processing this amount is over 5 billion won per year.

Therefore, various attempts have been made to deal with such garbage in the past. For example, Korean Patent Registration No. 10-0775589 (Registration Date: Nov. 5, 2007) discloses a method for preparing a deodorant using condensate extracted from food waste and the deodorant obtained thereby. A method of obtaining a deodorant by fermenting is proposed. However, according to this technique, the condensed water condensed with steam generated by boiling at high temperature is used instead of using the food waste as it is, so that the apparatus for the manufacture thereof is complicated and its cost increases considerably.

Therefore, it would be advantageous if there was a new deodorant manufacturing technology that can be manufactured at low cost while cultivating microorganisms using waste.

The present invention has been invented in order to improve the technology of producing the deodorant by treating the conventional waste described above and to provide various additional advantages. The present invention is based on the inventors' insight into the efficient production of a deodorant at a lower cost if the agricultural by-products generated in large quantities in the agricultural and fish market are disposed of as waste, and by using agricultural by-products such as cabbage, cabbage, lettuce, etc. It is an object of the present invention to provide a method for producing a lactic acid deodorant which can utilize the sterilizing power and deodorizing power of organic acids, biologics, bacteriocine, hydrogen peroxide, etc. contained in the culture medium after culturing the lactic acid bacteria.

In addition, the present invention is to provide a cheap natural lactic acid deodorant by combining the masking effect and the deodorizing power of the rich aroma generated by combining with the inherent flavor of the lactic acid bacteria culture medium by adding a flavoring to the lactic acid bacteria culture medium fermented lactic acid bacteria by recycling agricultural by-products The purpose.

The above object is achieved by a method for preparing a lactic acid deodorant using an agricultural by-product provided in accordance with the present invention and a lactic acid deodorant thereof.

The manufacturing method provided according to an aspect of the present invention is a method for preparing a lactic acid deodorant using a crushed liquid of agricultural by-products, yeast extract, white sugar, trisodium citrate, sodium phosphate, purified water in the crushed liquid of agricultural by-products Culturing the lactic acid bacteria by inoculating and culturing by inoculating lactic acid bacteria spawn after mixing and making a medium (medium) to sterilize; An acidity adjustment step of adjusting the lactic acid concentration to be 1.5% to 2% by removing the remaining dust from the lactic acid bacteria culture medium produced in the lactic acid bacteria culture step, mixing purified water with the remaining solution, and adding lactic acid concentrate stock solution; And a finishing step of sterilizing the lactic acid bacteria culture medium in which the acidity is adjusted in the acidity adjusting step.

In one embodiment of the present invention, the step of culturing the lactic acid bacteria farming byproducts crushing step (S011) for crushing the agricultural by-products generated from at least one of cabbage, cabbage, lettuce; 45 ~ 55% by weight of the agricultural by-products produced in the agricultural by-products shredding step, 0.5 ~ 1.5% by weight of yeast extract, 3.5 ~ 4.5% by weight per white, trisodium citrate 0.5 ~ 1.5% by weight, sodium phosphate 0.5 ~ 1.5 weight Mixing the raw materials with 40% to 60% by weight of purified water to prepare a medium for culturing lactic acid bacteria (medium) and mixing step; A sterilization step of treating the medium at a temperature of 100 to 130 ° C. for 10 to 30 minutes, and then slowly cooling to 30 ° C. or less at room temperature; Lactobacillus spawn is inoculated with 1-2% by weight of the medium (medium) treated by the sterilization step and then incubated for 5 to 7 days at 20 ~ 30 ℃ spawn inoculation and culture step to produce a lactic acid bacteria culture solution. .

In one embodiment of the present invention, the acidity adjustment step is a shredding dust removal step of removing the remaining dry to the lactic acid bacteria culture medium produced in the lactic acid bacteria culture step using a bag filter or centrifuge and leaving only the liquid lactic acid bacteria culture liquid Wow; After mixing the purified water twice the weight of the purified liquid lactic acid bacteria culture medium, 1.0 ~ 1.5% by weight of lactic acid concentrate stock solution of 90 ~ 100% concentration to the weight of the mixed solution, finally lactic acid concentration of 1.5% ~ And a lactic acid level adjustment step of adjusting to 2.0%.

In one embodiment of the present invention, the finishing step is a sterilization step of sterilizing by treating the acidity adjusted lactic acid bacteria in the acidity adjustment step 1 minute to 3 minutes at 100 ℃; It includes a flavoring step of mixing the sterilized lactic acid bacteria culture medium and flavoring generally distributed in a ratio of 100: 1.

The agricultural by-product consists of by-products generated from at least one of cabbage, cabbage and lettuce.

Provided is a lactic acid deodorant prepared by a method for preparing lactic acid deodorant using the agricultural by-products described above.

According to the present invention having the above-described configuration, the present invention has the effect of providing a method for producing a high efficiency deodorant at a lower cost while reducing waste disposal costs by recycling a large amount of agricultural by-products treated with waste in the agricultural and fishery market to provide.

According to the present invention, by cultivating the lactic acid bacteria using agricultural by-products such as cabbage, cabbage, lettuce, etc., the efficiency of using the sterilizing power and deodorizing power of organic acids, biopharmaceuticals, bacteriocine, hydrogen peroxide, etc. contained in the lactic acid bacteria culture medium It can provide a high lactic acid deodorant.

In addition, the present invention by adding a fragrance to the culture medium fermented with lactic acid bacteria by recycling the agricultural by-products by combining the deodorizing power and masking effect of the rich aroma generated by combining with the inherent aroma of the lactic acid bacteria culture medium to provide a cheap lactic acid deodorant To provide.

Conventional deodorants may feel sticky after being dried mostly due to organic solvents or organic residues, but the lactic acid deodorant according to the present invention continuously kills harmful bacteria while evaporating moisture and does not become sticky at all after evaporation. Lactobacillus enzymes have a deodorizing effect that essentially eliminates odors, and the scent can last more than three days in a closed space.

In addition, agricultural by-products, which are used as materials, can be used as animal feed additives or soil fertilizers by separating large particles in the process of producing lactic acid bacteria culture medium, thus providing a great effect in preventing environmental pollution.

Hereinafter, with reference to the accompanying drawings illustrating the present invention with a specific example as follows.

1 is a flow chart showing each step of the method for producing a lactic acid deodorant using agricultural by-products according to an embodiment of the present invention, Figure 2 is a graph of the acidity change of the cultured lactic acid bacteria liquid by time using the juice of the cabbage by-products, Figure 3 Is a graph illustrating the number of bacteria before and after the application of lactic acid culture liquid, which is a lactic acid deodorant obtained by a method for producing lactic acid deodorant using agricultural by-products, on commercially available bean sprouts.

1 is a process for producing a lactic acid deodorant, characterized in that the production of lactic acid deodorant using a crushed liquid of agricultural by-products according to the present invention, the step of culturing the lactic acid bacteria (S01), the adjustment step of acidic acid culture medium (S02) and finishing It consists of step S03.

Culture step (S01) of lactic acid bacteria, yeast extract, white sugar, trisodium citrate, sodium monophosphate, purified water mixed with the crushed solution of the agricultural by-products to make a medium, after sterilization, inoculated with lactic acid bacteria spawn incubation step Finally, the lactic acid bacteria culture medium is obtained.

Acidity adjustment step (S02), the bactericidal power of the lactic acid bacteria culture medium is determined, remove the remaining residues in the lactic acid bacteria culture medium made in the lactic acid bacteria culture step (S01), and mixed with purified water to the remaining solution and lactic acid concentrate stock solution is added to lactic acid The concentration is adjusted so that the concentration is 1.5% to 2.0%.

Finishing step (03), and finally to complete the lactic acid deodorant in the form of a liquid lactobacillus culture medium, in the acidity adjustment step (S02) sterilized by treating the acidity-adjusted lactic acid culture medium at 100 ° C for 1 minute to 3 minutes, It is the step of adding fragrance for the additional masking effect with the deodorizing power of the lactic acid culture liquid.

Referring to Figure 1 to describe in more detail each process as follows.

Culture step (S01) of lactic acid bacteria, agricultural by-products shredding step (S011), medium raw material input and mixing step (S012), sterilization step (S013), spawn inoculation and culture step (S014).

The agricultural by-product crushing step (S011) is to produce a crushed juice or juice of agricultural by-products, crushed or juiced by crushing or juice at least one of the by-products, such as cabbage, cabbage, lettuce. The resulting dust does not need to be removed to be recycled as compost.

The medium raw material input and mixing step (S012) is to prepare a medium for the culture of lactic acid bacteria, crushed liquid produced in the agricultural by-product shredding step (S011) 45 ~ 55% by weight, yeast extract 0.5 ~ 1.5% by weight, white sugar 3.5 to 4.5% by weight, trisodium citrate 0.5 to 1.5% by weight, sodium monophosphate 0.5 to 1.5% by weight, purified water 40 to 60% by weight to prepare a medium for culturing lactic acid bacteria.

The sterilization step (S013) is to remove other microorganisms that are not necessary, and after the culture solution is treated for 10 to 30 minutes at a temperature of 100 ~ 130 ℃ using an autoclave, slowly left at room temperature below 30 ℃ It is the cooling stage. This is a step of removing unnecessary microorganisms before inoculating the lactic acid bacteria.

The spawn seeding and culturing step (S014) is to prepare a lactic acid bacteria culture medium, and then inoculated with 1 to 2% by weight of the weight of the medium treated by the sterilization step (S013) 5 ~ 20 ~ 30 ℃ Incubate for 7 days to prepare a lactic acid bacteria culture medium.

Since the sterilization ability of the lactic acid bacteria culture medium increases as the acidity is increased, it is preferable to use a lactic acid concentration of 1.5% or more. However, matching the concentration of lactic acid by cultivation may require an unnecessary cost since the adjustment of a large-scale facility and a precise culture environment is necessary, so that the lactic acid bacteria culture solution obtained in the lactic acid bacteria culture step (S01) is then acidified. It is preferable to adjust so as to have an appropriate acidity in S02).

The acidity adjustment step (S02) consists of a shredding dust removal step (S021) and a lactic acidity adjustment step (S022).

The shredding removal step (S021) is to purify only the liquid lactobacillus culture solution, and the remaining lactic acid bacteria culture solution made in the lactic acid bacteria culture step (S01) by using a bag filter or centrifuge, and only the liquid lactobacillus culture solution This is the step of purification to remain. The dry matter removed during this purification can be collected separately and recycled as animal feed additives or soil compost.

Lactic acidity adjustment step (S022) is to finally adjust the lactic acid concentration, adding 0.5 ~ 2.5% by weight of lactic acid concentrate stock solution of 90 to 100% concentration, purified water by mixing two times the purified liquid lactic acid bacteria culture medium The lactic acid concentration is adjusted so that the concentration is 1.5% to 2.0%. The adjusted lactic acidity can finally determine the deodorizing power of the lactic acid deodorant.

The finishing step S03 comprises a sterilizing step S031 and a flavoring step S032. The prepared lactic acid deodorant, that is, lactic acid bacteria culture medium is commercialized by the finishing step (S03).

Sterilization step (S031) is to maintain the acidity (that is, lactic acidity), the acidity adjusted in the acidity adjustment step (S02) and sterilized by treating the lactic acid bacteria culture medium adjusted for 1 minute to 3 minutes at a temperature of 100 ℃ ~ 120 ℃ Therefore, the lactic acid bacteria culture medium is no longer contained in the microorganisms and after that the acidity can be maintained.

The fragrance step (S032) is for the deodorizing power and masking effect of the dark scent, and the advantage of being able to combine the deodorizing power and masking effect of the strong scent by mixing the sterilized lactic acid bacteria culture medium and the flavor generally distributed in a ratio of 100: 1. This is provided.

Figure 2 is a graph showing the change in pH and pH of the lactic acid bacteria culture medium cultured using the juice of the cabbage by-products over time.

The curve of the hourly lactic acidity of FIG. 2 and the curve of the PH indicate numerical changes of the hourly acidity of Table 1 and the hourly pH of Table 2.

Table 1 shows the relationship between the bactericidal power and lactic acidity of the lactic acid bacteria culture medium according to the change of acidity over time.

      Lactic acid bacteria have an inhibitory effect on pathogenic microorganisms and other harmful microorganisms, and organic acids (mainly lactic acid and acetic acid), hydrogen peroxide, and bacteriocin that are produced during the growth of lactic acid bacteria have inhibitory effects. have. Therefore, the higher the concentration of the organic acid having such a harmful microorganism inhibition mechanism in the production of lactic acid bacteria cultures can be said to have a higher deodorizing and sterilizing effect.

In view of the deodorization and sterilization effect of the lactic acid bacteria culture as described above, the increase in acidity according to the lactic acid bacteria culture medium described in Table 1, the increase in acidity according to the lactic acid bacteria culture time during the process of the culture of the lactic acid bacteria cultured for 144 hours, It can be seen that it has a great effect on the bactericidal power.

Table 2 is a numerical expression of the curve of the pH appearing over time, the pH value decreases over time in the process of culturing the lactic acid bacteria culture medium will affect the culture rate. This affects the incubation time of the culture time and can also have a great influence on the sterilization power. To prevent this, it is necessary to use a buffer that prevents a sharp decrease in pH in a short time. In the case of culturing lactic acid bacteria without using a buffer, the pH rapidly decreases over time, which causes the growth of lactic acid bacteria to be inhibited, and the production of organic acids is lowered, which leads to a very slow increase in lactic acid. Therefore, by adding sodium phosphate and citric acid as a buffer to buffer the sudden change in pH, it is possible to increase the sterilization power by appropriately adjusting the culture rate and incubation time.

time 23 44 60 68 85 128 144 Acidity 0.4 0.9 One 1.1 1.2 1.3 1.5

time 23 44 60 68 85 128 PH 5 4 4 3.8 3.7 3.5

Figure 3 is applied to commercially available bean sprouts using a lactic acid culture solution to examine the bactericidal effect on bacteria, (a) and (b) shows the results of detecting the number of bacteria before and after applying the lactic acid deodorant of the present invention The graph is a comparison, Table 3 is a comparative value of the bactericidal effect of the lactic acid deodorant.

Specifically, in order to investigate the sterilization effect of the lactic acid deodorant in the general bacterial count shown in the purchased bean sprouts sample A, a portion of the bean sprouts was quantitatively placed in a sterile bag, and sterilized water was added. The bacteria contained in the bean sprouts were separated and included in the water. Then, the water was inoculated into a plain plate medium and incubated at room temperature for 72 hours, and the number of bacteria was found to be 3.3 ㅧ 10 ⁷ cfu / g. The lactic acid deodorant with lactic acid 1.5 was applied to the cultured plain plate medium, and then treated with a spray for about 2 minutes, washed with washing water, and then cultured at room temperature for 3 days, and the number of bacteria was 3,100 cfu / g. .

As a result, as shown in Figure 3 (b) and Table 3, after applying the lactic acid deodorant of the present invention 2 minutes treatment it can be seen that more than 99.99% sterilization effect.

In the purchased bean sprout sample B, the general bacterial count of the bean sprout sample A obtained through the same process was found to be 1.4 ㅧ 10 ⁸ cfu / g. The lactic acid deodorant with lactic acid 1.5 was applied to the cultured plain plate medium, and then treated with a spray for about 2 minutes, washed with washing water, and then cultured for 3 days at room temperature. The bacterial count was 24,000 cfu / g. . As a result, as shown in Figure 3 (b) and Table 3, it can be seen that after applying the lactic acid deodorant of the present invention for 2 minutes treatment more than 99.98% bactericidal effect.

Sample Lactic acidity Incubation time
(h) Processing time
(min) Number of bacteria before application
(cfu / g) Number of bacteria after application
(cfu / g) Sterilization effect
(%) A 1.5 72 2 3.3 ㅧ 10 ⁷
 3,100 99.99 B 1.5 72 2 1.4 ㅧ 10 ⁸ 24,000 99.98

<Examples>

Production of lactic acid bacteria culture medium 45% by weight of crushed by-products of cabbage, cabbage, lettuce, yeast extract 0.5% by weight, white sugar 3.5% by weight, trisodium citrate 0.5% by weight, sodium monophosphate 0.5%, purified water 50% by weight After mixing, the lactic acid bacteria culture medium is sterilized at 121 ° C. for 15 minutes using an autoclave to obtain only the seed material necessary for cultivation and completely kills the remaining bacteria. The seed of the completely sterilized culture medium was slowly cooled to 30 ° C. or lower, and then aseptically inoculated with 1.5% by weight of the total weight of the lactic acid bacteria culture medium, followed by incubation at room temperature (25 W 5 ° C.) for 6 days.

After purifying only the supernatant using a bag filter or a centrifuge to remove large particles of debris from the cultured lactic acid bacteria culture medium, the supernatant was mixed with purified water 1: 2 and concentrated to lactic acid to obtain efficacy of at least 99.99% bactericidal power. 1.0 wt% of the stock solution (90% to 100%) was mixed to make lactic acid concentration 1.5% to 2.0%.

After boiled for 1 minute at 100 ℃ sterilized by adding 1.0% by weight of flavor and evenly mixed, to prepare a final deodorant (or lactic acid bacteria culture).

Although the present invention has been described through specific embodiments, various modifications are possible to those skilled in the art by referring to and combining various features described herein. Therefore, the scope of the present invention should not be limited to the described embodiments, but should be interpreted by the appended claims.

As described above, a method for preparing lactic acid deodorant using agricultural by-products according to the present invention and its lactic acid deodorant are widely used in the field of recycling and deodorant of agricultural by-products treated as waste.

1 is a flow chart showing each step of the method for producing a lactic acid deodorant using agricultural by-product waste according to an embodiment of the present invention.

Figure 2 is a graph showing the change in the concentration and pH of lactic acid by culture time of the lactic acid bacteria culture medium cultured by a method for producing a lactic acid deodorant using agricultural by-product waste according to an embodiment of the present invention.

Figure 3 is a graph illustrating the number of bacteria before and after the application of lactic acid deodorant, a lactic acid deodorant obtained by the method for producing lactic acid deodorant using agricultural by-product waste in accordance with an embodiment of the present invention.

Claims (6)

In the method for producing a lactic acid deodorant by using a crushed liquid of agricultural by-products, the crushed liquid of the agricultural by-products are mixed with yeast extract, white sugar, trisodium citrate, sodium monophosphate, purified water to sterilize , Incubating the culture of lactic acid bacteria inoculated with lactic acid bacteria (S01); Acidity adjustment step of adjusting the lactic acid concentration to be 1.5% ~ 2% by removing the remaining dust from the lactic acid bacteria culture medium made in the lactic acid bacteria culture step (S01) and mixing purified water with the remaining solution and adding lactic acid concentrate stock solution (S02) Wow; Finishing step (S03) for sterilizing the acidity-adjusted lactic acid culture medium in the acidity adjustment step (S02) Characterized in that it comprises a lactic acid deodorant production method using agricultural by-products. The method according to claim 1, wherein the step of culturing the lactic acid bacteria (S01), Agricultural by-product shredding step (S011) of grinding agricultural by-products generated from at least one of cabbage, cabbage and lettuce; 45 ~ 55% by weight of the agricultural by-products produced in the agricultural by-products shredding step, 0.5 ~ 1.5% by weight of yeast extract, 3.5 ~ 4.5% by weight per white, trisodium citrate 0.5 ~ 1.5% by weight, sodium phosphate 0.5 ~ 1.5 weight %, The raw material input and mixing step (S012) to produce a medium (medium) for culturing lactic acid bacteria by mixing 40 to 60% by weight of purified water; After sterilizing the medium (medium) for 10 to 30 minutes at a temperature of 100 ~ 130 ℃, left at room temperature to sterilize slowly to 30 ℃ or less (S013) and; Seed inoculation and culture step of inoculating the lactic acid bacteria spawn in 1 to 2% by weight of the medium (medium) treated by the sterilization step and then incubated for 5 to 7 days at 20 ~ 30 ℃ to produce a lactic acid bacteria culture solution (S014) To; Lactic acid deodorant manufacturing method using agricultural by-products comprising a. The method of claim 2, wherein the acidity adjustment step (S02), A shredding dust removing step (S021) of purifying the lactic acid bacteria culture liquid produced in the lactic acid bacteria culture step using a bag filter or a centrifugal separator and purifying only the liquid lactic acid bacteria culture liquid to remain; After mixing two times the purified water of the purified liquid lactic acid bacteria culture medium, 1.0 to 1.5% by weight of lactic acid concentrate stock solution of 90 to 100% of the weight of the mixed solution was added, and finally the lactic acid concentration was 1.5% to 2.0%. Lactic acidity adjustment step (S022) to be adjusted to be; Lactic acid deodorant manufacturing method using agricultural by-products comprising a. The method of claim 3, wherein the finishing step (S03), A sterilization step (S031) of sterilizing by treating the lactic acid bacteria culture medium in which the acidity is adjusted in the acidity adjusting step at 100 ° C. for 1 minute to 3 minutes; A flavor step (S032) of mixing the sterilized lactic acid bacteria culture medium and the fragrance in a ratio of 100: 1; Lactic acid deodorant manufacturing method using agricultural by-products comprising a. The method of claim 1, wherein the agricultural by-products are made of by-products generated from at least one of cabbage, cabbage, and lettuce. Lactic acid deodorant prepared by any one of claims 1 to 5.

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