CN106635902B - Bacillus coagulans and application thereof - Google Patents

Bacillus coagulans and application thereof Download PDF

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CN106635902B
CN106635902B CN201611196824.6A CN201611196824A CN106635902B CN 106635902 B CN106635902 B CN 106635902B CN 201611196824 A CN201611196824 A CN 201611196824A CN 106635902 B CN106635902 B CN 106635902B
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bacillus coagulans
livestock
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bacillus
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CN106635902A (en
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刘明刚
李坤
卢祯鑫
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Fujian Luodong Bio Technology Co ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L9/00—Disinfection, sterilisation or deodorisation of air
    • A61L9/01—Deodorant compositions
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D9/00—Other inorganic fertilisers
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2209/00—Aspects relating to disinfection, sterilisation or deodorisation of air
    • A61L2209/20—Method-related aspects
    • A61L2209/21—Use of chemical compounds for treating air or the like

Abstract

The invention relates to bacillus coagulans preserved in CCTCC with a preservation number of CCTCC M2016440 and application thereof in livestock digestion. The bacillus coagulans has strong lactic acid producing capacity, promotes the digestion of livestock on feed, and can be applied to the aspect of livestock feeding. The bacillus coagulans can secrete a large amount of lactic acid, effectively inhibit the growth of harmful bacteria, improve the intestinal micro-ecological environment, promote the intestinal development, enhance the intestinal function and promote the digestion and absorption of livestock and poultry on feed. Meanwhile, the bacillus coagulans has excellent environmental stress resistance, effectively inhibits the growth of harmful bacteria, quickly reduces ammonia in the environment, improves the culture environment, and can be used for harmless treatment of livestock and poultry carcasses and deodorization and bacteriostasis of farms. The invention also provides a composition for innocent treatment of the livestock and poultry carcasses and a composition for deodorization and bacteriostasis of a farm.

Description

Bacillus coagulans and application thereof
Technical Field
The invention relates to the field of microorganism application, in particular to bacillus coagulans and application thereof.
Background
Bacillus coagulans (Bacillus coagulans) is gram-positive and belongs to the phylum of hard (or thick) walled bacteria. Bacillus coagulans is classified as a Bacillus, and has rod-shaped cells, gram-positive bacteria, terminal spores and no flagella. Decomposing saccharide to generate L-lactic acid, which is homolactic ferments. The optimum growth temperature is 45-50 deg.C, and the optimum pH is 6.6-7.0. The bacillus coagulans is facultative anaerobe, can grow in aerobic and anaerobic environments, can adapt to hypoxic intestinal environments, has high tolerance to acid and bile, can be subjected to lactic acid fermentation, and can generate L-lactic acid which can reduce the pH value of intestinal tracts, inhibit harmful bacteria and promote the growth and reproduction of beneficial bacteria such as bifidobacterium and the like. Bacillus coagulans is able to form spores, which is beneficial for restoring the microecological balance of the gastrointestinal tract compared to other non-lactic acid producing bacilli.
At present, the microbial feed additive is a biological agent prepared by fermenting beneficial microbes approved by the Ministry of agriculture, and is the best substitute of antibiotics. The artificial culture bacteria and metabolite thereof for improving health level of livestock and poultry can adjust microecological imbalance in livestock and poultry, maintain microecological balance, and is a microbial product for stimulating propagation and growth of beneficial bacteria of animals and simultaneously resisting growth of harmful bacteria. The main problems with microbial feed additives are the selection of good bacterial species and the reduction of microbial feed additive activity due to inactivation of bacterial species.
China is a big livestock breeding country, and when the livestock industry develops, the number of livestock dead due to natural death and diseases is quite large every day because of the large base number of livestock feeds. The source of the animal carcass can be divided into two forms of natural elimination or natural death and disease death according to the death reasons of the animals. The elimination hazard of natural death is small, and the improper treatment of animal carcasses died due to epidemic diseases not only seriously pollutes water sources, soil and atmosphere, but also easily causes the spread of the epidemic diseases. Infected animal carcasses with strong infectivity, such as foot-and-mouth disease, swine fever, highly pathogenic avian influenza and the like, even seriously threaten the life and health of human beings. The traditional treatment of dead bodies of animals mainly comprises a landfill method, a disinfection method, an incineration method, a chemical preparation method and a biodegradation method (fermentation method). Wherein the animal carcasses are treated by biodegradation, but the treatment time for the carcasses is too long or malodors are generated.
Disclosure of Invention
The invention provides the bacillus coagulans with strong lactic acid production capacity through research, screening and verification on the bacillus coagulans, and the bacillus coagulans strain and the composition containing the bacillus coagulans strain can be effectively applied to production of feed additives, deodorization and bacteriostasis of farms and harmless treatment of dead livestock and poultry carcasses.
For the first purpose of the invention, the invention provides a culture collection center CCTCC (China center for culture Collection) of the microbial strains with the preservation numbers as follows: bacillus coagulans strain CCTCC M2016440. The preservation date is as follows: 2016, 9, 1, latten name by category: bacillus Coaglans, the Bacillus Coagulans of the invention has strong lactic acid producing capability and can promote the digestion of livestock on feed.
In order to achieve the second object of the present invention, the present invention provides the use of Bacillus coagulans deposited in CCTCC with a preservation number of CCTCC M2016440 in livestock breeding. During the feeding process, bacillus coagulans preserved in CCTCC with the preservation number of CCTCC M2016440 is added into animal feed or drinking water.
The bacillus coagulans preserved in CCTCC with the preservation number of CCTCC M2016440 can secrete a large amount of lactic acid, effectively inhibit the growth of harmful bacteria, improve the intestinal micro-ecological environment, promote the intestinal development, enhance the intestinal function and promote the digestion and absorption of livestock and poultry on feed.
In order to achieve the third object of the present invention, the present invention provides a microbial feed additive, which comprises bacillus coagulans preserved in CCTCC and having a preservation number of CCTCC M2016440 as an effective ingredient.
The bacillus coagulans preserved in CCTCC with the preservation number of CCTCC M2016440 can secrete a large amount of lactic acid, effectively inhibit the growth of harmful bacteria, improve the intestinal micro-ecological environment, promote the intestinal development, enhance the intestinal function and promote the digestion and absorption of livestock and poultry on feed.
In order to achieve the fourth object of the present invention, the present invention provides a farm deodorizing and bacteriostatic composition, which comprises bacillus coagulans with a preservation number of CCTCC M2016440 as an effective ingredient.
Further, the plant deodorization and bacteriostasis composition comprises 50 parts by weight of bacillus coagulans with a preservation number of CCTCC M2016440, 100 parts by weight of bacillus subtilis with a preservation number of CCTCC M2016441, 100 parts by weight of bacillus subtilis with a preservation number of CCTCC M2016443 and750 parts by weight of montmorillonite powder. Wherein the viable count of Bacillus coagulans with a preservation number of CCTCCM2016440 is 1.0 × 109CFU/g～5.0×109CFU/g, preservation number of CCTCC M2016441 viable count of Bacillus subtilis is 2.0 × 109CFU/g～1.0×1010CFU/g, preservation number of CCTCC M2016443, viable count of Bacillus subtilis is 5.0 × 108CFU/g～5.0×109CFU/g。
The bacillus coagulans preserved in CCTCC with the preservation number of CCTCC M2016440 has excellent environmental stress resistance, effectively inhibits the growth of harmful bacteria, quickly reduces ammonia in the environment and improves the culture environment.
In order to achieve the fifth object of the present invention, the present invention provides a composition for the harmless treatment of livestock and poultry carcasses, which comprises bacillus coagulans as an active ingredient, wherein the bacillus coagulans is preserved in CCTCC with a preservation number of CCTCC M2016440.
Further, the composition for harmless treatment of the livestock and poultry carcasses comprises 100 parts by weight of bacillus coagulans with a preservation number of CCTCC 2016440, 100 parts by weight of bacillus subtilis with a preservation number of CCTCC M2016441, 100 parts by weight of bacillus subtilis with a preservation number of CCTCC M2016443 and 700 parts by weight of zeolite powder. Wherein the viable count of Bacillus coagulans with a preservation number of CCTCCM2016440 is 2.0 × 108CFU/g～1.0×109CFU/g, preservation number of CCTCC M2016441, viable count of Bacillus subtilis is 3.0 × 109CFU/g～1.0×1010CFU/g, preservation number of CCTCC M2016443, viable count of Bacillus subtilis is 1.0 × 109CFU/g～1.0×1010CFU/g。
The bacillus coagulans preserved in CCTCC with the preservation number of CCTCC M2016440 has excellent environmental stress resistance, effectively inhibits the growth of harmful bacteria, can efficiently generate organic acid reaction, quickly reduces ammonia in the environment and improves the environment.
Detailed Description
In order to explain technical contents, structural features, and objects and effects of the technical means in detail, the following detailed description is given with reference to specific embodiments.
The bacillus coagulans preserved in CCTCC with the preservation number of CCTCC M2016440 is a bacillus coagulans strain which is obtained by mutation in solid state fermentation culture of the inventor, and is separated and screened from the culture, wherein the bacillus coagulans strain has the growth speed which is more than 1 time faster than that of an ATCC (American type culture Collection) 7050 model strain and has strong lactic acid production capacity.
The bacterial strain has rod-shaped cells, single cells and pairs, and the surfaces of bacterial colonies are slightly wet, matt, non-protruding, flat, opaque and white; the middle slightly thick edge is thinner, the edge is expanded, and the edge is round or spread into a wave shape and an irregular shape; in the culture medium lining: the colony is punctiform, and transparent rings are arranged around the colony; observation under a microscope: the cell is rod-shaped, the two ends of the cell are blunt and round, spores are formed, and flagella are generated at the ends of the spores; endospore formation, gram positive (G +), facultative anaerobic, and fermentable to produce large quantities of antibacterial coagulans, lactic acid, amino acids, vitamins, and various digestive enzymes. The Bacillus coagulans has strong lactic acid producing ability, and can promote digestion of livestock and poultry on feed.
In the screening, the samples used were selected variants of the ATCC (American type culture Collection) 7050 model strain in the following medium: 1000ml of distilled water, 2.5g of yeast extract powder, 5.0g of peptone, 1.0g of glucose, 801.0 g of Tween, 0.1g of L-cysteine, 0.06g of bromocresol purple and 15.0g of agar, and the pH value is controlled to be 7.0.
A diluted solution was prepared from 0.85% by mass/volume NaCl and 0.05% Tween 80 in 1000ml of distilled water. And diluting the sample with the diluting solution to obtain a sample solution.
After the sample solution is coated on the culture medium, the sample solution is cultured for 72 hours at 37 ℃ in an anaerobic incubator, colonies with good growth vigor, similar colony morphology and the largest number are selected, and the single bacterium is separated and purified until the colony morphology is consistent.
And (4) carrying out a test in a lactic acid detection test on the separated single bacteria, and screening the single bacteria with the highest lactic acid yield. Under microaerobic conditions, when the glucose content is 5.00g/L, the acid yield is 1.50 g/L. The screened strain is bacillus coagulans preserved in CCTCC with the preservation number of CCTCC M2016440.
The screened strains are stored in a sand tube and a freeze drying tube and are in a dormant state. When in use, the strain needs to be activated and cultured to obtain a certain amount of pure strains.
The method for culturing the bacillus coagulans comprises the following steps of:
a. activating strains: inoculating production strain in dormant state into test tube to activate slant
b. And (3) strain amplification culture: sequentially carrying out primary seed shake cultivation, secondary seed tank cultivation, tertiary seed tank cultivation and fermentation tank enlarged cultivation on the strains.
The culture medium for strain expansion culture comprises the following components: corn starch, soybean meal, corn steep liquor, yeast extract powder, K2HPO4Peptone, MgSO4.7H2O; the pH of the medium was 7.0.
Example 1 Chicken farm test
720 helan brown laying hens of 31 weeks old are selected for the test, the breeding hens are randomly divided into 3 groups, the control group 1, the control group 2 and the test group are 240 respectively, and the test period is 21 days. And (3) testing daily ration: 61.5 percent of corn, 23.4 percent of soybean meal, 2 percent of wheat bran, 8.1 percent of stone powder and 5 percent of compound premix, and the health-care antibiotics are not fed in the test period.
The control group 1 was not inoculated, and the other feeding conditions were the same; control group 2 and test group were supplemented with 0.1% o of microbial feed additive in the feed during the test period. Wherein the control group 2 microbial feed additive comprises Bacillus subtilis with a preservation number of 1% CCTCCM2016442 (viable count of 1.0 × 10)9cfu/g) and 99% glucose, the microbial feed additive of the test group is prepared from 1% of bacillus subtilis with a preservation number of CCTCC M2016442 (the viable count is 1.0 multiplied by 10)9cfu/g), 0.1% Bacillus coagulans with preservation number CCTCC M2016440 (viable count is 1.0 × 10)8cfu/g), 98.9% glucose.
The test results are as follows:
item Egg weight (g) Laying rate (%) Percentage of broken eggs (%) Percentage of eggs lost (%) Daily consumptive material (g) Material to egg ratio
Control group 1 63.4±1.25 87.3±4.29 0.68±0.34 0.44±0.33 125.2±9.3 2.26
Control group 2 63.0±1.54 87.7±3.86 0.57±0.28 0.45±0.28 120.2±5.5 2.18
Test set 63.3±0.72 87.7±2.18 0.49±0.15 0.42±0.25 118.7±4.9 2.15
The test results show that the single egg weight and the laying rate of the test group are not obviously different from those of the control group, but the standard deviation is more stable, which indicates that the addition of bacillus coagulans with the preservation number of CCTCC M2016440 can improve the stability of laying eggs. In the aspect of feed utilization, the daily feed consumption of a test group is reduced by 5.2 percent compared with that of a control group 1, the feed-egg ratio is reduced by 4.9 percent, and the effect is more obvious than that of the control group. The result shows that on the basis of the bacillus subtilis with the preservation number of CCTCC M2016442, the bacillus coagulans with the preservation number of CCTCC M2016440 is added, the egg laying stability can be enhanced, and the feed utilization rate can be improved.
Example 2 nursery pig feeding test
Randomly selecting 3 nursery pigs in a pig farm nursing house, wherein 20 pigs are selected in each pig farm, 2 pigs are used as a control group, and 1 pig is used as a test group.
Control group 1 used a feed without microbial additives and was fed with chlortetracycline, acidulant and sweetener for health during the test period.
The control group 2 is prepared by adding 0.1% of microorganism feed additive into feed, wherein the microorganism feed additive comprises 10g Bacillus subtilis (with preservation number of CCTCC M2016442, viable count of 1.0 × 109cfu/g) and 990g of glucose. No chlortetracycline, acidulant and sweetener for health use were used during the test period.
The test group added 0.1% of microbial feed additive into the feed, the microbial feed additive comprises 1g of Bacillus coagulans (viable count is 1.0 × 10) with preservation number of CCTCC M20164408cfu/g), 10g Bacillus subtilis (viable count is 1.0 × 10) with preservation number CCTCCM20164429cfu/g) and 989g of glucose. No chlortetracycline, acidulant and sweetener for health use were used during the test period.
After feeding the nursery pigs for 31 days, the nursery pigs were tested and no mortality occurred in each group, and the results are shown in the following table:
Figure GDA0002180305110000061
the test results show that the average daily gain of the test group is higher than that of the control group 1 and that of the control group 2, the average daily feed intake of the test group is not obviously different from that of the control group, and is lower than that of the control group 1, and the feed-meat ratio of the test group is lower than that of the control group 1 and that of the control group 2. In the test process, the odor of the excrement discharged by the test group is obviously lower than that of the control group 1 and the control group 2, and the odor in the colony house in the culture process can be reduced.
Example 3A composition for deodorization and bacteriostasis in a farm
50 parts by weight of Bacillus coagulans (viable count 2.0 × 10) with preservation number of CCTCC M20164409cfu/g), 100 parts by weight of bacillus subtilis with the preservation number of CCTCC M2016441 (the viable count is 1 multiplied by 10)10cfu/g), 100 parts by weight of bacillus subtilis with the preservation number of CCTCC M2016443 (the viable count is 1 multiplied by 10)9cfu/g) and 750 parts by weight of montmorillonite powder are uniformly mixed to prepare the deodorizing and bacteriostatic composition for the farm.
Example 4 hog house deodorizing bacteriostatic test
Four pigsties are selected as a test object in a pig farm, the pigsties 1 are a control group 1, the pigsties 2 are a control group 2, the pigsties 3 are a control group 3, the pigsties 4 are a test group, the areas and the space sizes of the four pigsties are the same, and about 1000 fattening pigs are respectively arranged in the pigsties.
Control 1 was sprayed with clear water;
the control group 2 used 100g Bacillus subtilis (viable count 1 × 10) with preservation number of CCTCC M201644110cfu/g) and 900g of montmorillonite powder are evenly mixed and dissolved in a reference solvent 2 prepared by 100L of clear water;
the control group 3 adopts 100g Bacillus subtilis (viable count is 1 × 10) with preservation number of CCTCC M201644110cfu/g), 100g of bacillus subtilis with the preservation number of CCTCC M2016443 (the viable count is 1 multiplied by 10)9cfu/g), and 800g of montmorillonite powder, and dissolving in 100L of clear water to prepare a control solvent 3.
Test group 1kg of the farm deodorizing and bacteriostatic composition prepared in example 3 was dissolved in 100L of test solvent prepared in clean water.
The deodorizing solvent was uniformly sprayed on the floor and wall of the pigsty by a sprayer, and after 5 days, the concentration of the odor and ammonia gas was measured, and the results are shown in the following table:
Figure GDA0002180305110000071
Figure GDA0002180305110000081
wherein, the odor concentration adopts a three-point comparison odor bag method (GB/T14675), and the ammonia gas adopts a sodium hypochlorite-salicylic acid spectrophotometry (GB/T14679).
After 5 days of the test, the number of coliform plates in the feeding area, the feces discharging area and the pigsty wall of the pigsty were counted, and the sampling area was 25cm2. The results are given in the following table:
detection area Control group 1 Control group 2 Control group 3 Test set
Feeding area 1.5×106CFU 3.3×105CFU 1.6×103CFU 1.3×103CFU
Defecation area 6.1×106CFU 9.7×105CFU 3.2×104CFU 4.7×104CFU
Pigsty wall 3.9×105CFU 5.2×104CFU 8.7×102CFU 6.4×102CFU
The test result shows that: the experimental group bacteriostatic agent is added with the bacillus coagulans with the preservation number of CCTCC M2016440, can obviously reduce the ammonia gas in the environment compared with a control group, and simultaneously, the bacterium added has a certain inhibition effect on the coliform flora in the environment.
Example 5A composition for innocent treatment of carcasses of livestock and poultry
100 parts by weight of Bacillus coagulans (viable count is 1.0 × 10) with preservation number of CCTCC M20164409cfu/g), 100 parts by weight of bacillus subtilis with the preservation number of CCTCC M2016441 (the viable count is 1.0 multiplied by 10)10cfu/g), 100 parts by weight of bacillus subtilis with the preservation number of CCTCC M2016443 (the viable count is 2.0 multiplied by 10)9cfu/g) and 700 parts by weight of zeolite powder are uniformly mixed to prepare the composition for harmless treatment of the livestock and poultry carcasses.
Example 6 innocent treatment of carcasses of livestock and poultry
Selecting 40 dead chickens and 20 dead pig bodies, dividing into 4 parts with the same mass, wherein 3 parts are used as a control group, 1 part is used as a test group, and each part is added into 5 cubic meters of padding consisting of sawdust and rice hull matrix.
In the control group 1, 1000g of zeolite powder was added to the padding;
control group 2 to the bedding, 100g of Bacillus subtilis (viable count 1.0 × 10) with preservation number of CCTCC M2016441 was added10cfu/g) and 900g of zeolite powder;
control group 3 to the bedding, 100g of Bacillus subtilis (viable count 1.0 × 10) with preservation number of CCTCC M2016441 was added10cfu/g) and 100g of Bacillus subtilis with the preservation number of CCTCC M2016443 (the viable count is 2.0 multiplied by 10)9cfu/g) and 800g of zeolite powder;
test group to bedding was added 1000g of a composition prepared in example 5 for the harmless treatment of carcasses of livestock and poultry.
Sampling and testing after compost fermentation is completed, and detecting that the control group 1 contains salmonella and the detection results of fowl plague and hog cholera virus are negative. The test group, the control group 3 and the control group 2 do not detect salmonella, and the detection results of fowl plague and swine fever virus are negative.
The control group 1 took 26 days to complete the fermentation, and the fermentation process produced foul smell; the control group 2 took 10 days to complete the fermentation, but the oil in the padding material was only decomposed a little, and ammonia odor and a little odor were generated in the fermentation process; the control group 3 takes 10 days to complete fermentation, the grease in the padding is decomposed after fermentation, the padding is not greasy, and ammonia odor and a small amount of odor are generated in the fermentation process; and the test group finishes fermentation for 10 days, the padding is not greasy after fermentation, and odor and ammonia gas are hardly generated in the fermentation process.
The result shows that the addition of the bacillus coagulans with the preservation number of CCTCC M2016440 obviously reduces the emission of ammonia gas and improves the comfort level in the manual operation process.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or terminal that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or terminal. Without further limitation, an element defined by the phrases "comprising … …" or "comprising … …" does not exclude the presence of additional elements in a process, method, article, or terminal that comprises the element. Further, herein, "greater than," "less than," "more than," and the like are understood to exclude the present numbers; the terms "above", "below", "within" and the like are to be understood as including the number.
Although the embodiments have been described, once the basic inventive concept is obtained, other variations and modifications of these embodiments can be made by those skilled in the art, so that the above embodiments are only examples of the present invention, and not intended to limit the scope of the present invention, and all the modifications of the equivalent structure or equivalent flow path using the present specification, or the direct or indirect application to other related fields are included in the scope of the present invention.

Claims (6)

1. Bacillus coagulans preserved in CCTCC with a preservation number of CCTCC M2016440 (A)Bacillus Coagulans） 。
2. A microbial feed additive characterized by containing the Bacillus coagulans strain of claim 1 as an active ingredient.
3. A composition for deodorizing and inhibiting bacteria in a farm, which comprises the Bacillus coagulans of claim 1 as an active ingredient.
4. The composition for deodorization and bacteriostasis in livestock farms according to claim 3, which comprises Bacillus coagulans of claim 1, Bacillus subtilis with the collection number of CCTCC M2016441 (wherein the Bacillus coagulans is Bacillus subtilis) (the collection number of which is CCTCC M2016441)Bacillus Subtilis) And Bacillus subtilis with the preservation number of CCTCC M2016443 (Bacillus Subtilis） 。
5. A composition for innocent treatment of livestock and poultry carcasses, which comprises the bacillus coagulans of claim 1 as an active ingredient.
6. The composition for the innocent treatment of livestock and poultry carcasses according to claim 5, which comprises the bacillus coagulans of claim 1, the bacillus subtilis with the preservation number of CCTCC M2016441 and the bacillus subtilis with the preservation number of CCTCC M2016443.
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