CN107400637A - A kind of bacillus coagulans and its microorganism formulation, preparation method and application - Google Patents

A kind of bacillus coagulans and its microorganism formulation, preparation method and application Download PDF

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CN107400637A
CN107400637A CN201710258715.0A CN201710258715A CN107400637A CN 107400637 A CN107400637 A CN 107400637A CN 201710258715 A CN201710258715 A CN 201710258715A CN 107400637 A CN107400637 A CN 107400637A
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bacillus coagulans
microorganism formulation
daoduo
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唐鸿志
许平
杨科武
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Shanghai Daodo Biological Technology Co ltd
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Abstract

The invention discloses a bacillus coagulans (Bacillus coagulans) Daoduo 4 and its screening technique, preparation method and application comprising the microorganism formulation of the bacterial strain and the microorganism formulation are also disclosed.The microorganism formulation of the present invention, culture is simple, Stability Analysis of Structures, it is nontoxic to have no side effect, the breeding of the harmful microbes such as spoilage organisms, pathogen and growth can effectively be suppressed, it is stronger to the resistance of environment, there is high temperature resistant, it is acidproof, the advantages that resistance to choline, stink can be eliminated in the growth of the harmful bacteria during complex condition suppresses rubbish, excrement and sewage, pollution is reduced, improves environment.On the other hand, it can effectively remove VOC (VOC), applied to places such as newly-decorated houses, can significantly reduce finishing pollution and be endangered to caused by health.

Description

A kind of bacillus coagulans and its microorganism formulation, preparation method and application
The cross reference of related application
First Chinese patent Shen of the application based on 2 months 2017 Application No. 2017101046947 submitted for 24th Please, and the priority of this application is required, is herein incorporated the entire disclosure of which by quoting.
Technical field
The present invention relates to environment environmental technology field, more particularly to a kind of bacillus coagulans, its screening technique, with And microorganism formulation containing the bacillus coagulans, the preparation method and application of the microorganism formulation.
Background technology
As China's Urbanization Progress is rapid, the level of urbanization is steadily improved, and the size of population is continuously increased, urban life Environmental pollution has attracted people's attention caused by rubbish.Garbage collection points and the house refuse of garbage transfer station accumulation, The problems such as initiation stench is distributed, and mosquitos and flies is multiplied, and pest and disease damage is propagated, causes necessarily to quality of residents'life and local environment Influence.The preliminary deodorizing process of domestic waste, have become an important ring for urban environmental protection ecological construction.
Currently, there is problems with urban garbage disposal process：
(1) organic matter in the rubbish stacked rots to decompose, and can produce substantial amounts of foul gas, make one nausea, it is dizzy and Vomiting.Stench main source is organic principle in rubbish in ammonia, hydrogen sulfide, first caused by chemistry and biodegradation The escaping gases such as mercaptan are extremely harmful to health.
(2) house refuse is the collection source of disease microorganism, and some pathogenic microorganisms are the sources for producing stink.In the summer Under its hot conditions, the microbial fermentation in house refuse forms strong stench, serious threat to environmental sanitation and people's Health.
(3) refuse landfill is substantially carried out the last processing of house refuse, is the main aggregation of domestic waste, And the place of stench most serious.The stench source of landfill yard is multi-point and wide-ranging, entirely transported from household refuse landfill sites reservoir area, Processing links have substantial amounts of foul gas to produce discharge, and especially container is in the discharging of landfill operation face, substantial amounts of fermentation Liquid flows out first, and the foul gas of adjoint high concentration distributes, and instantaneous stench release concentration is high；Rubbish after dumping is pushing away Native machine, excavator stirring under, the foul gas in rubbish space is further emitted in air, is diffused into air flow In surrounding enviroment；When landfill operation every morning face cover layer is raised, the effluvium of high concentration can be also given out under film Body.
With global warming, " tropical island effect " aggravation, a large amount of foul gas caused by the house refuse in city are difficult To dissipate.In current Prediction for Solid Waste Treatment Technology both at home and abroad, it is difficult to the elimination stench of continuation, especially in summer high-temperature condition Under, foul gas is iteratively produced, and the concentration for controlling foul gas for a long time, effect are difficult to the methods of common Physical, chemical method Fruit can only continue very short time, and cost is high, effect is poor, it is impossible to persistently solves stink pollution problem, it is difficult to adapt in current rubbish Turn the requirement of station and refuse landfill.
On the other hand, in recent years, with the increasingly raising of people's living standard, and the quickening of urbanization process, occur More and more high-grade office buildings of finishing and luxurious room, there occurs great variety indoor environment.The closing in many rooms Formula is fitted up causes serious air pollution problem inside rooms with the ornament materials used in interior.Wherein, VOC (VOC) there is tremendous influence to health.When the VOC in room reaches finite concentration, people can feel head in the short time Bitterly, Nausea and vomiting, weak etc., twitch, stupor occur when serious, and liver, kidney, brain and the nerve of people can be hurt System, cause the serious consequences such as failure of memory.It is reported that the coating used during decoration design for residence is indoor VOC One of main source.So each state is all limited the VOC content in the decorating and renovating materials such as coating.Existing market is deposited Microbial deodorant preparation almost without decomposition can be played to volatile organic thing VOC.
Therefore, those skilled in the art is directed to developing a kind of environment deodorant microbial agent, to can be in complicated bar Foul smell and VOC VOC concentration are effectively reduced under part, reduces poor environment to living quality and health Influence.
The content of the invention
The present invention is difficult under the conditions of complicated (high temperature, hyperosmosis) for foul gas inorganic agent of the prior art Effectively suppress the deficiency of harmful bacterial growth for a long time, there is provided a kind of micro- life of deodorization for being efficiently applied to complicated adverse circumstances Thing preparation, it can be under high temperature hyperosmosis environment, efficient-decomposition stench harmful substance, reduces releasing for ammonia and hydrogen sulfide High-volume, foul odour is removed for a long time.On the other hand, it can effectively remove VOC (VOC), applied to new The places such as the house of finishing, finishing pollution can be significantly reduced and endangered to caused by health.Concrete technical scheme is as follows：
The present invention provides a bacillus coagulans in first aspect, is Bacillus coagulans Daoduo 4, China typical culture collection center, address were preserved on 2 21st, 2017：Wuhan, China Wuhan University, preserving number are CCTCC NO:M 2017054。
Preferably, the screening technique of above-mentioned bacillus coagulans includes：
1) prepare and contain CaCO3The MRS solid plates of emulsion；
2) microbiological specimens are gathered from slowly drained soil, by above-mentioned micro- life under conditions of 30-60 DEG C (being preferably 50 DEG C) Thing sample is rule on MRS solid plates, selects the bacterium colony containing molten calcium circle；
3) rule repeatedly on MRS solid plates, obtain single bacterial strain.
The present invention provides a kind of microorganism formulation in second aspect, and it includes bacillus coagulans as described above Daoduo 4。
Preferably, effective total viable count >=1.0 × 10 in the microorganism formulation9CFU/mL。
The present invention provides above-mentioned bacillus coagulans or microorganism formulation in deodorization and except volatility has in the third aspect Application in machine compound.
The present invention provides the preparation method of mentioned microorganism preparation in fourth aspect, comprises the following steps：
Step 1, flat board culture：Using solid activation medium, flat board activation bacillus coagulans Daoduo 4, formed Single bacterium colony；
Step 2, shake-flask seed liquid make：Use liquid activation medium in shaking flask, the condensation bud in incubation step 1 Spore bacillus Daoduo 4, form shake-flask seed liquid；
Step 3, domestication culture：Culture medium is tamed using liquid, tames the bacillus coagulans in incubation step 2 Daoduo 4；
Step 4, fermented and cultured：Using fermentation tank and fermentation medium, the condensation bud after being tamed in fermented and cultured step 3 Spore bacillus Daoduo 4, microorganism formulation is obtained after fermentation.
Preferably, the program of above-mentioned flat board culture is：Bacillus coagulans Daoduo 4 is inoculated with using streak inoculation method In on solid activation medium flat board, 36~48h is cultivated in 50 DEG C, bacillus coagulans Daoduo 4 is recovered, forms single bacterium Fall.
Preferably, the program of above-mentioned shake-flask seed liquid making is：It will be grown fine with oese in step 1 free of contamination Single bacterium colony is inoculated into liquid activation medium, 30 DEG C~35 DEG C, 36~48h of shaken cultivation under the conditions of 150~200rpm, is obtained To shake-flask seed liquid.
Preferably, the program of above-mentioned domestication culture is：The shake-flask seed liquid of step 2 is accessed in liquid tames culture medium Domestication culture is carried out, inoculum concentration is 2~5%, and condition of culture is 50 DEG C~55 DEG C, 180~200rpm shaken cultivations, 36~ 48h, passed on for two generations.
Preferably, the program of above-mentioned fermented and cultured is：Fermentation medium is added in fermentation tank, is taken after being tamed in step 3 Bacillus coagulans Daoduo 4 be inoculated with, inoculum concentration 2%~4%, ferment under normal temperature 72~96h.
Preferably, aforesaid liquid domestication culture medium and fermentation medium contain 2%~4% molasses, and wherein molasses are sugarcane Molasses, it is sugar industry byproduct.
Preferably, the application method of mentioned microorganism preparation includes the microorganism formulation pressing 1:10~1:50 ratio Dilution, deodorization object is treated in uniformly sprinkling, daily to spray once, continuous sprinkling 3 days~5 days.
Present invention also offers a kind of deodorant, preferably garbage deodorant, and a kind of finishing pollution thing inorganic agent, They contain above-mentioned bacillus coagulans Daoduo 4 respectively.
The bacillus coagulans Daoduo 4 of the present invention is a kind of safe microorganisms strain, harmless, to environment not Second is produced to pollute., can be raw under complicated severe environment and it has the advantages that high temperature resistant, acidproof, bile tolerance It is long.Subtilin, polymyxins, nystatin, gramicidins isoreactivity material are produced in its growth course, to pathogenic bacteria There is obvious inhibitory action；A large amount of enzymes can also be produced, can the strongly ability of decomposing organic matter and foul gas.It is same with this When, condense gemma flora release lactic acid, acetic acid and some can suppress the antibiotic and antibioticses thing of harmful bacteria growth and breeding Matter, effectively suppress the breeding of putrefactive microorganisms, promote beneficial microorganism growth, improve the microbiologic population in environment.
Therefore, the microorganism formulation with bacillus coagulans Daoduo 4 for principal component, can be integrated from multiple angles Deodorization is carried out to the place of rubbish or other environmental malodors, can be suppressed under the conditions of complicated (high temperature, hyperosmosis) harmful thin Bacteria growing, from Sources controlling rubbish odor pollution, and good deodorizing effect can be obtained.Especially under summer high-temperature weather, The microorganism formulation dilution of the present invention is sprayed on the places such as garbage transfer station, refuse landfill and plant, can be in air Ammonia, the odorant such as hydrogen sulfide play the effect such as absorption, enzymolysis, suppress harmful microbe growth, can effectively eliminate and be difficult to Naturally the foul odour to dissipate, stink does not recur within a couple of days, so as to play the improvement result to environment.
Raw material or reagent used in the present invention is commercially available in addition to special instruction.The present invention is compared to existing skill Art, there is provided it is a kind of to remove the lasting microorganism formulation with comprehensive function of waste odor, effect from multiple angles, make up Conventional garbage processing biological agent removes the defects of waste odor is ineffective, continuation is bad, avoids common rubbish and removes The secondary pollution risk and bio-safety risk that smelly preparation is brought.It can be in the place such as garbage transfer station and refuse landfill Peculiar smell is removed, in the presence of the present invention, the foul gas of rubbish can be reduced rapidly, suppress the breeding of putrefactivebacteria, It is met the safety standard in country and place, improve the micro-ecological environment of rubbish heap, there is great economic implications and ring Protect meaning.This product is used for rubbish deodorization, the formation of comprehensive regulation rubbish foul gas and diffusion problem, ecological, environmental protective, makes With conveniently, production cost is low, to environment non-secondary pollution, is harmful to human health, has broad application prospects.The opposing party Face, it can effectively remove VOC (VOC), applied to places such as newly-decorated houses, can significantly reduce finishing Pollution harm to caused by health.
Brief description of the drawings
Fig. 1 is the pseudomonas putida Daoduo 4 of present invention 16S rRNA gene sequencing results.
Embodiment
The bacillus coagulans of embodiment 1 (Bacillus coagulans) Daoduo 4 screening and identification
Preparation contains CaCO3The MRS solid plates of emulsion, gather microbiological specimens from slowly drained soil, in 50 DEG C of bar Sample is selected into the bacterium colony containing molten calcium circle in above-mentioned flat lining out under part, repeatedly in above-mentioned flat lining out, obtained single One bacterial strain.
Wherein, MRS culture medium prescriptions (1L)：Peptone 10g, beef extract 10g, yeast extract 5g, K2HPO42g, lemon Acid three ammonium 2g, sodium acetate 5g, glucose 20g, Tween 80 1mL, MgSO4·7H2O 0.5g, MnSO4·4H2O 0.25g。
Institute of Microorganism, Academia Sinica is sent to carry out bacterial strain identification one plant of bacterial strain for screening acquisition, according to bacterial strain The experimental data comprehensive analysis such as cellular morphology, bio-chemical characteristics, 16S rRNA gene orders, reference《Primary Jie Shi Systems Bacterials Learn to do volume》And International Journal of Systematic and Evolutionary Microbiology Relevant research paper, qualification result are bacillus coagulans (Bacillus coagulans).
1st, cellular morphology and physical and chemical experiment result are as shown in table 1：
The cellular morphology and physical and chemical experiment result of the bacterium of table 1
2nd, 16S rRNA gene sequencings result, as shown in Figure 1.
3rd, antibiotic sensitive experimental result, as shown in table 2.
The antibiotic sensitive experimental result of the bacterium of table 2
The above-mentioned bacterial strains for screening acquisition are named as Bacillus coagulans Daoduo 4, in 2 months 2017 21 Day is preserved in China typical culture collection center, and preserving number is CCTCC NO:M 2017054.
The cultural method of the bacterial strain is：By bacterial strain, (1L) carries out enrichment culture in LB culture mediums, as seed liquor, turns It is connected to the culture medium (remaining composition is water) containing molasses 2~4% and carries out large-scale culture (800L), about 72~96h at normal temperatures Effective total viable count >=1.0 × 109CFU/mL.Compared to other cultural methods, this method has extremely strong economic benefit, into This is cheap, and strain growth situation is no less than enriched medium, while bacterial strain does not allow denaturation easy to aging, so as to farthest real The function of its existing deodorization.
Deodorant microbial agent of embodiment 2 and preparation method thereof
Include bacillus coagulans Daoduo 4 deodorant microbial agent, said preparation is fermented type tart flavour, pH value 4 ~5, effective total viable count >=1.0 × 109CFU/mL, wherein, main effectively viable bacteria bacillus coagulans Daoduo 4 is 109~ 1011CFU/mL。
The preparation method of deodorant microbial agent is as follows：
(1) flat board culture, solid activation medium, flat board activation bacillus coagulans Daoduo 4 are used
Bacillus coagulans Daoduo 4 is inoculated on solid activation medium flat board using streak inoculation method, in 50 DEG C 36~48h of culture, makes its recovery, forms single bacterium colony.
Wherein solid activation medium is prepared according to following weight percentage：Peptone 1%, dusty yeast 0.5%, chlorination Sodium 1%, agar 2%, remaining is distilled water, 121 DEG C of sterilizing 20min, is cooled down standby.
(2) shake-flask seed makes, using shaking flask and liquid activation medium, the bacillus coagulans in incubation step (1) Daoduo 4
The free of contamination strain that grown fine in step (1) is inoculated into liquid activation medium respectively with oese, with 36~48h of shaken cultivation under the conditions of 30 DEG C~35 DEG C of 500mL triangular flasks, 150~200rpm, obtains shake-flask seed liquid, and sterile Operation sampling carries out bacterial strain identification, it is ensured that bacterial strain is correct.
Wherein liquid activation medium is prepared according to following weight percentage：Peptone 1%, dusty yeast 0.5%, chlorination Sodium 1%, remaining is distilled water, 121 DEG C of sterilizing 20min, is cooled down standby.It is worth noting that, yeast is added in shake-flask seed liquid Powder is to obtain more preferable, the more viable count of state in seed liquor in a short time, is trained in order to which the later stage amplifies Support.
(3) domestication culture, culture medium is tamed using liquid, tames the bacillus coagulans Daoduo in incubation step (2) 4 single strains
Liquid domestication culture medium is added in the big triangular flasks of 1L, the single strain of access step (2) carries out domestication culture, connect Kind amount is 2~5%, and condition of culture is 50 DEG C~55 DEG C, 18~200rpm shaken cultivations, 36~48h, passes on for two generations.
Culture medium is wherein tamed to prepare according to following weight percentage：Molasses 2%~4%, dusty yeast 0.5%, pH 6.0 ~6.5, remaining is 115 DEG C of sterilizing 15min of distilled water, and cooling is standby.
(4) fermented and cultured, it is solidifying after being tamed in fermented and cultured step (3) using stainless steel fermentation tank and fermentation medium Bacillus Daoduo 4 is tied, prepares the microorganism formulation for rubbish deodorization
Fermentation medium 800L is added in 1 ton of stainless steel fermentation tank with agitating device, takes domestication training in step (3) The liquid bacterial strain supported is inoculated with, inoculum concentration 2%~4%, and ferment 72~96h under normal temperature, is calculated under the microscope after 72h Viable count, when effective total viable count >=1.0 × 109During CFU/mL, stop stirring, that is, prepare deodorant microbial agent.
Wherein fermentation medium is prepared according to following weight percentage：Molasses 2%~4%, remaining is distilled water, without PH value is adjusted, 75 DEG C of 2h that sterilize are standby after being quickly cooled to 35 DEG C.
The application method of the microorganism formulation is：1 is pressed with the water of cleaning:10~1:50 dilution proportion, uniformly sprinkling are treated Deodorization object, daily sprinkling are once, continuous to spray 3 days~5 days.
Embodiment 3 carries out deodorization engineering using mentioned microorganism preparation in the refuse landfill of Suzhou one
Using mentioned microorganism preparation, with 1:50 ratio, it is configured to 500 kilograms of deodorant liquids.
In the present embodiment, gaseous sample collection is carried out to the household refuse landfill sites scope of operation, with odor concentration and volatilization Property organic compound VOC be index, the change of combined reaction environmental malodors.
Odor concentration be reflection people to the index of the subjective feeling degree of stench, determined using triangle odor bag method, Dimensionless.Ammonia uses solution absorption-spectrophotometry, and VOC is determined using gas chromatography, and unit is mg/m3, The change of environmental gas stench is caused from quantitative angle reflection.
This analysis method to VOC in sample (VOC) content is HJ 732-2014 and USEPA TO-15 (1999) two methods, wherein HJ 732-2014 (PAMS) include 57 kinds of compounds, and TO-15 includes 50 kinds of compounds, It is 112 kinds altogether along with sulfur-containing compound (hydrogen sulfide, methyl sulfide, methyl mercaptan, methyl disulfide), nitrogen-containing compound (ammonia) Material.112 kinds of odorants in this gas analysis sample method are divided into sulfur-containing compound, nitrogen-containing compound, hydro carbons Compound, halogenated hydrocarbon compound, benzene compounds, ether ketone compound and the class of VFA compounds seven, the material title included per one kind It is shown in Table 3.
VOC material classifications in the house refuse stench Sources controlling industrial experimentation of table 3：
In work flow, using giant to the microorganism formulation after rubbish sprinkling dilution, it is ensured that often load one layer Rubbish, spray a certain amount of deodorant, accomplish medicament sufficiently and uniformly with domestic garbage mixing.Use 500 kilograms of deodorant liquids Operated, sprayed 3 times, and to doing odor concentration and VOC material comparative analysis before and after deodorization.
After microbial deodorant using the present invention, odor concentration and VOC VOC change such as table 4 It is shown：
The odor concentration of table 4 and VOC changes
Time of application Temperature Odor concentration VOC
0min 18℃ 353-759 1.5-2.2
10min 18℃ 250-555 0.9-1.6
30min 20℃ 159-368 0.3-0.9
5h 28℃ 122-279 0.2-0.6
Odor concentration and VOC VOC concentration after processing are significantly less than country and each department issue Relevant criterion.To showing up at an elevated temperature, odor concentration and VOC concentration persistently increase, and sprinkling is above-mentioned micro- The experimental group of biological agent is to continue to reduce in the elevated situation odor concentration of temperature and VOC concentration.Illustrate the microorganism system Agent energy continuous and effective reduces odor concentration, so as to eliminate odor pollution, at the same time, moreover it is possible to the degraded volatility of continuous and effective Organic compound VOC, it is ensured that rubbish Environmental security, the microorganism formulation use food grade materials culture, nontoxic spray completely Spill it is rear tasteless, it is harmless, do not produce second of pollution to environment.
Embodiment 4 carries out deodorization application using mentioned microorganism preparation in the refuse landfill of Nanjing one
The refuse landfill of Nanjing one, (produced using mentioned microorganism microbial inoculum with compareing microorganism formulation by Jiangsu company Silver leaf microbial deodorant) carry out deodorizing effect contrast.
By the microorganism formulation containing bacillus coagulans Daoduo 4 prepared in embodiment 2 with compareing microorganism system Agent is all according to 1:30 same ratios are diluted with running water, are made into the deodorizing microorganism of working concentration.The soot scope of operation is uniform Spray, spraying area (dimensionless), take at random a little at 4, carry out sampling test, as a result as illustrated in tables 5-7.
Microorganism formulation test data of the table 5 containing bacillus coagulans Daoduo 4
Table 6 compares microorganism formulation test data
7 two kinds of product average treatment efficiency comparisons of table
Pass through average treatment efficiency comparison, it is seen that microbial inoculum of the present invention can effectively reduce odor pollution, to volatile organic Compound has nearly 100% elimination effect.
Application of garbage deodorant of the embodiment 5 containing pseudomonas putida Daoduo 4 in rubbish from cooking is removed
Rubbish from cooking refers to that resident's daily life and food processing, catering service, unit such as are served the meals at the caused rubbish in activity Rubbish, including abandon no dish leaf, leftovers, leftovers, pericarp, eggshell, tea grounds, bone etc., its main source be family kitchen, Dining room, restaurant, dining room, market and other industries relevant with food processing.Rubbish from cooking is different from common rubbish, has group Divide the features such as complicated, moisture and content of organics are high, it is easy to addle, produce stench.The main component of its foul smell has vulcanization Hydrogen, methyl mercaptan, 1,3- dimethyl benzenes, ortho-xylene, methyl disulfide and paraxylene etc..Due to food waste class rubbish foul smell into Point more special, the deodorization agent concentration needed during processing is higher, it is necessary to dilute 10~20 times or so uses.
In the present embodiment, using the categorized rubbish from cooking of the garbage transfer station of Nanjing one as object, removed using above-mentioned rubbish Smelly dose carries out deodorizing effect contrast with compareing microorganism formulation (the silver leaf microbial deodorant produced by Jiangsu company).Will Two kinds of deodorant are according to 1:15 ratio is diluted with running water, is made into the deodorizing microorganism of working concentration, average treatment effect As shown in table 8
8 two kinds of product average treatment efficiency comparisons of table
Pass through average treatment efficiency comparison, it is seen that microbial inoculum of the present invention can effectively reduce the odor pollution of rubbish from cooking, to waving Hair property organic compound has nearly 100% elimination effect.
Finishing pollution thing inorganic agent of the embodiment 6 containing bacillus coagulans Daoduo 4 removes VOC Application
The measure of indoor air purification effect is carried out in the present embodiment using following methods：
1st, by blank control reagent (water) and be diluted with water 40 times of finishing pollution thing inorganic agent distinguished with Sprayable it is equal Even to be sprayed in blank test cabin A and sampling test cabin B, fountain height is identical.
2nd, the container equipped with the VOC pollutant source of releases prepared is respectively placed in blank test cabin A and sampling test cabin In B, hatch door is closed immediately.
3rd, blank test cabin A and sampling test cabin B fan is opened, stirs 1mim, air in cabin is discharged with source of release Pollutant mix after, simultaneously close off fan, to carrying out air sampling in blank cabin, pollutants in air in measure blank cabin Concentration is initial concentration, is denoted as C0。
3rd, the concentration of air pollutants in two cabins is sampled respectively after 0.5h, 1h, 2h, 4h, 12h, 72h, analysis is surveyed Examination, the concentration value in A, B cabin, is denoted as C as in certain time periodAWith CB。
Clearance can be calculated as follows：
Y=(CA CB)/CA×100
In formula：
Y clearances, %；
CABlank test cabin pollutant concentration value, mg/m3；
CBSampling test cabin pollutant concentration value, mg/m3。
Result of the test is as shown in table 9：
Clearance of the finishing pollution thing inorganic agent of table 9 to VOC
Time 0.5h 1h 2h 4h 12h 72h
Clearance 88.2% 90.3% 92.9% 93.8% 94.0% 96.3%
It can be seen from the results above that the finishing pollution thing provided by the invention containing bacillus coagulans Daoduo 4 Inorganic agent has remarkable result to removing VOC VOC, and it works quickly, and duration length, clearance approaches 100%, effect is applied in newly-decorated house far better than the like product of in the market, can mitigate emulsion paint significantly The harm brought Deng finishing material to human body.
Preferred embodiment and comparative example of the invention described in detail above.It should be appreciated that the common skill of this area Art makes many modifications and variations without creative work can according to the design of the present invention.Therefore, it is all in the art Technical staff under this invention's idea on the basis of existing technology can be with by logical analysis, reasoning, or a limited experiment Obtained technical scheme, all should be in the protection domain being defined in the patent claims.

Claims (10)

  1. A 1. bacillus coagulans, it is characterised in that the bacillus coagulans is bacillus coagulans (Bacillus Coagulans) Daoduo 4, China typical culture collection center, preserving number CCTCC were preserved on 2 21st, 2017 M 2017054。
  2. 2. the screening technique of bacillus coagulans as claimed in claim 1, it is characterised in that the screening technique includes：
    1) prepare and contain CaCO3The MRS solid plates of emulsion；
    2) microbiological specimens are gathered from slowly drained soil, consolidate the microbiological specimens in the MRS under conditions of 30-60 DEG C The flat lining out of body, select the bacterium colony containing molten calcium circle；
    3) rule repeatedly on the MRS solid plates, obtain single bacterial strain.
  3. 3. a kind of microorganism formulation, it is characterised in that the microorganism formulation includes condensation gemma bar as claimed in claim 1 Bacterium.
  4. 4. microorganism formulation as claimed in claim 3, it is characterised in that effective total viable count in the microorganism formulation >= 1.0×109CFU/mL。
  5. 5. the preparation method of the microorganism formulation as described in claim 3 or 4, it is characterised in that methods described includes：
    Step 1, flat board culture：Using solid activation medium, flat board activates the bacillus coagulans Daoduo4, is formed single Bacterium colony；
    Step 2, shake-flask seed liquid make：Use liquid activation medium in shaking flask, the condensation gemma in incubation step 1 Bacillus Daoduo 4, form shake-flask seed liquid；
    Step 3, domestication culture：Culture medium is tamed using liquid, tames the bacillus coagulans Daoduo in incubation step 2 4；
    Step 4, fermented and cultured：Using fermentation tank and fermentation medium, the condensation gemma after being tamed in fermented and cultured step 3 Bacillus Daoduo 4, the microorganism formulation is obtained after fermentation.
  6. 6. the preparation method of microorganism formulation as claimed in claim 5, it is characterised in that the program of the flat board culture is： The bacillus coagulans Daoduo 4 is inoculated on the solid activation medium flat board using streak inoculation method, in 50 DEG C 36~48h is cultivated, the bacillus coagulans Daoduo 4 is recovered, forms single bacterium colony.
  7. 7. the preparation method of microorganism formulation as claimed in claim 5, it is characterised in that the liquid domestication culture medium and institute State the molasses that fermentation medium contains 2%~4%.
  8. 8. the application method of the microorganism formulation as described in claim 3 or 4, it is characterised in that the application method includes will The microorganism formulation presses 1:10~1:50 dilution proportion, deodorization object is treated in uniformly sprinkling, daily to spray once, continuous sprinkling 3 days~5 days.
  9. 9. a kind of deodorant, it is characterised in that the deodorant includes bacillus coagulans as claimed in claim 1.
  10. 10. a kind of finishing pollution thing inorganic agent, it is characterised in that the finishing pollution thing inorganic agent is included such as claim 1 institute The bacillus coagulans stated.
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CN109078480A (en) * 2018-09-06 2018-12-25 荣成市华通海洋生物科技有限公司 Bacillus coagulans apply in fish meal production exhaust air deodorization as deodorization bacterium solution, deodorization bacterium solution preparation method and exhaust air deodorization device

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