KR100439635B1 - Novel microorganism which effectively enhances animal performances and reduces smell of animal excretions, agent containing thereof and preparation method of the same - Google Patents

Abstract

The present invention relates to a microbial agent for feed addition and a method for manufacturing the same, and more particularly, to a microbial agent for animal feed addition and a method for producing the same for reducing the generation of odor due to livestock increase, feed efficiency and livestock manure. More specifically, unlike the conventional muker microorganism that has been mainly used for food and alcohol production, such as cheese or soy sauce, a new muker strain KFCC-11239 has been identified as having an efficiency of suppressing odor by the increase of livestock and livestock manure. The microorganisms of this muker are used alone or as a mixture of several microorganisms that play a role in promoting the release of intestinal harmful toxins and the activity of useful bacteria in the intestine. It can greatly contribute to income growth.

Description

Novel microorganism which effectively enhances animal performances and reduces smell of animal excretions, agent containing etc and preparation method of the same}

The present invention relates to a microbial agent for feed addition and a method for manufacturing the same, and more particularly, to a microbial agent for animal feed addition and a method for producing the same for reducing the generation of odor due to livestock increase, feed efficiency and livestock manure.

The microbial agent for livestock feed is a substance containing microorganisms to balance the intestinal flora of livestock and increase the productivity of livestock according to the increase of digestion of feed. There is a demand for a microbial agent that can be reduced.

Therefore, the study on the increase in livestock productivity and the study for eliminating odor are disclosed in Korean Patent Publication No. 1998-14276, Korean Patent Publication No. 1998-56775, Korean Patent Publication No. 1999-80373, Korean Patent Publication No. 2000-73915 Reported in the issue. However, Korean Patent Publication No. 1998-14276 and Korean Patent Publication No. 1998-56775 relate to the composting cost microorganisms that house organic wastes, and there is no mention or suggestion of deodorizing microorganisms using molds. It is also limited to the removal of sulfur-derived substances and ammonia. Patent Publication No. 1999-80373 relates to a microbial agent for feed addition containing lactic acid bacteria, yeast, and Aspergillus duckling, and its effect is not known by the type of cattle only by identifying the effect of only Korean cattle, and Patent Publication No. 2000-73915 The heading relates to microorganisms for livestock containing Bacillus, lactic acid bacteria and yeast, and does not contain molds which are commonly known to produce a large amount of bioactive substances. Therefore, as described above, the microbial agent for animal feed additives or odor removing microorganisms containing mold has not been reported until now.

Accordingly, the present inventors have completed the present invention by successfully separating new microorganisms which effectively reduce the occurrence of odor in livestock manure as well as improving the productivity of livestock after the continuous and multi-faceted research.

SUMMARY OF THE INVENTION An object of the present invention is to increase the productivity when feeding a livestock feed and to reduce the odor of livestock manure to suppress the occurrence of respiratory diseases, and microbial agent containing the microorganisms of the Muker and the microorganisms of the species together with It is to provide a complex microbial agent containing other microorganisms.

Another object of the present invention is to provide a method for producing the microbial agent.

In addition, another object of the present invention is to provide a novel muker genus microorganism having an effect of improving the productivity and malodor decomposition of livestock.

Hereinafter, the present invention will be described in more detail.

The present invention relates to a microbial agent containing a microorganism in the Mucor (mucor) that can suppress the occurrence of respiratory diseases by increasing the productivity of the livestock and at the same time reduce the occurrence of odor caused by livestock manure. The microorganism may be used alone or as a microorganism of the fungus, lactic acid bacteria, yeast, Bacillus genus such as Aspergillus oryzae .

The present invention also relates to a method for producing the microbial agent at low cost. In general, the microbial agent may be prepared by a liquid culture, a solid culture, a semi-solid culture, or the like. In the microbial culture, it is preferable to prepare a microbial liquid culture method that is easy to control and has a short incubation time. Incubate by

In addition, the present invention relates to a novel microorganism of the genus Muker strain KFCC-11239, which has an effect of improving productivity and reducing odor of livestock manure when fed to livestock.

In order to achieve the object of the present invention, the present inventors have succeeded in separating fungi that have the effect of increasing the productivity of livestock and reducing the odor of livestock manure, and the isolated fungus has been identified as a microorganism in the muker.

The fungus in the muker is called a fur fungus, and its structure is spore cysts upright and elongated in the mycelium, with unbranched and branched spores, with spore cysts at their ends. There is a characteristic of forming endogenous spores without forming. Depending on the species, it is used to produce an enzyme called Lenin, which replaces Rennet, a cheese curd enzyme, and microorganisms in the muker isolated from Nuruk in China are used for alcohol production because of its high starch glycation and alcohol fermentation power [Introduction to applied microbiology, p38] -39, Sejinsa, (1997)]. As described above, microorganisms in mukers and techniques using the same are mainly used for food and alcohol production, such as cheese and soy sauce, and have not been used as microorganisms or odor removing microorganisms or strains for feed addition.

Referring to the identification and characteristics of the novel microorganism disclosed in the present invention.

Strains of the present invention were isolated from traditional fermented foods by fungus, yeast and Bacillus fermentation such as soy sauce, Cheonggukjang. Traditionally, the physiological activity of fermented foods is a well known fact. Especially, the strains are fed to livestock based on molds, which are known to have high enzyme activity and produce a large amount of physiologically active substances. Now. First, various fermented foods were plated on PDA (Pato, Dextrose Agar) to isolate several fungal strains, and the results were identified by selecting strains with high enzyme productivity and the best odor reduction effect when added to livestock manure. The strain was identified as Muker genus. The strain of the present invention was deposited on November 23, 2000 with the accession number KFCC-11239 to the Korean spawn association.

The microorganism thus separated can be cultured in a medium such as PDA, dextrose peptone agar (DPA) or potato sucrose agar (PSA). At this time, the culture temperature is 25 to 37 ℃, more preferably at 37 ℃. pH conditions are suitable for 5.5-8.0, More preferably, pH 5.7. As the carbon source of the medium, glucose, starch, sucrose and the like can be used alone or in combination. As the nitrogen source, inorganic nitrogen sources such as ammonium sulfate and urea and organic nitrogen sources such as yeast extract, meat extract, and peptone may be used alone or in combination. The inorganic material may vary depending on the medium used, and it is generally preferable to use iron, magnesium, phosphorus, manganese, or the like.

In order to prepare the microbial agent of the present invention, the strain of the genus Muker obtained above is inoculated in a mold medium, preferably PDA medium, at 1 to 2 at 25 to 37 ° C., preferably at 37 ° C., at 100 to 200 rpm, preferably at 180 rpm. After aerobic incubation daily to obtain a culture, it is used as a seed.

The seed is inoculated into a fermentor containing a sterilized culture solution containing 1 to 3% by weight of glucose, preferably 2% by weight, 0.1 to 0.5% by weight of potato powder, and preferably 0.3% by weight to 25 to 37 ° C. Preferably, aerobic incubation at 37 ° C. for 1 to 2 days, preferably 2 days at 100 to 200 rpm, preferably 200 rpm.

In addition, one of the microorganisms contained in the microbial agent of the present invention is a microorganism of the genus Aspergillus . Examples include Aspergillus oryzae , Aspergillus niger , Aspergillus ficcum , and the like. Among them, Aspergillus duckling is known as Hwangkuk bacteria and has been widely used for the production of sake, soybean paste, soy sauce, sake, soju, etc., and has strong starch glycation and proteolytic ability. Among the above-mentioned Aspergillus sp. Microorganisms, Aspergillus duckase KFCC-60241 is particularly preferable.

Aspergillus duckling agent used as an example in the microbial agent of the present invention can be obtained by culturing in the same medium and culture conditions cultured the above-mentioned Muker strain.

As a member of another microbial agent, strains of the genus Bacillus are generally resistant to endocrine spores and are highly heat-resistant and useful for livestock, including cellulase, amylase, protease and xylanase. It is characterized by high productivity of enzymes such as fermentation products by Bacillus strains with Aspergillus aurise, which contains enzymes and other biologically active substances, which are useful for promoting intestinal beneficial flora and promoting livestock growth. It is known to do.

Examples of specific strains of the genus Bacillus include Bacillus licheniformis , Bacillus subtilis , Bacillus cereus , Bacillus amyloliquefaciens , and the like. Particularly preferred are Bacillus licheniform KCTC 1030 and Bacillus subtilis KCTC-1666. Such Bacillus strains can be cultured under conditions and medium commonly used for culturing Bacillus strains. For example, it can be incubated for 6 to 24 hours, preferably 12 hours at 30 to 40 ℃, preferably at 37 to 100 to 200rpm, preferably 180rpm in NB broth medium. In the case of mass production of this strain, it can be obtained by aerobic culture in a fermentor in which a medium containing 0.5 to 3% by weight of soybean meal, preferably 1% by weight and molasses 1 to 5% by weight, preferably 2% by weight is added. .

Another microbial source can be used as lactic acid bacteria, which refers to bacteria that produce more than 50% of the lactic acid production from glucose, lactic acid bacteria to feed livestock can be used mainly Lactobacillus ( Lactobacillus ) lactic acid bacteria. Examples include Lactobacillus acidophilus , Lactobacillus casei , Lactobacillus plantarum , Lactobacillus paracasei . In particular, Lactobacillus esidophilus ATCC 4356 is preferred. The role of lactic acid bacteria in the intestine of the livestock is to stabilize the intestinal microflora and to inhibit the productivity of livestock due to disease outbreaks by suppressing intestinal action, inhibiting the growth of harmful bacteria and stimulating the innate immune mechanism.

Such strains of lactic acid bacteria can be cultured under medium and conditions generally used for culturing Bacillus strains. For example, it may be incubated in MRS broth medium at 30 to 40 ° C., preferably at 37 ° C., at 80 to 150 rpm, preferably at 120 rpm for 12 to 48 hours, preferably 15 hours. In the case of mass production of the strain, yeast extract 0.1 to 1.0% by weight, preferably 0.5% by weight, glucose 0.3 to 1.0% by weight, preferably 0.7% by weight, and sodium acetate 0.1 to 0.5% by weight, preferably 0.2 It can be obtained by aerobic culture in a fermentor to which a medium containing a weight% is added.

Finally, yeast is a microorganism that produces alcohol and carbon dioxide using glucose as a carbon source, for example, Saccharomyces cerevisiae , Saccharomyces fermentati , Pichia sp.), Genus Hansenula sp., And Candida utilis . Saccharomyces cerevisiae is the preferred type of yeast that can be used in the present invention. In particular, ATCC 9763 is preferred for Saccharomyces cerevisiae. The cell wall of Saccharomyces cerevisiae used in the present invention is composed of functional polymer carbohydrates such as glucan, mannan and chitin, which binds harmful toxins and releases them to the outside of the body and supplies abundant amino acids, vitamins and unknown growth factors. It promotes the activity of useful bacteria, and in particular, the feed flavor is improved to play a role in raising the feed intake of livestock.

The yeast may be cultured under the general medium and conditions used for culturing yeast strains. For example, the YM broth medium may be incubated at 25 to 37 ° C., preferably at 37 ° C. at 50 to 150 rpm, preferably at 120 rpm for 15 to 48 hours, preferably 15 hours. In the case of mass production of the strain, soybean meal 0.5 to 3% by weight, preferably 1% by weight, molasses 0.1 to 2.0% by weight, preferably 0.5% by weight, glucose 0.5 to 3% by weight, preferably 1% by weight It can obtain by standing culturing in the fermentor which added the containing medium.

Table 1 shows the composition of the media used to prepare the microorganisms. The agar medium may be prepared by adding 1.5% by weight of agar to each medium of Table 1.

Kinds Component (g / L) PDA Broth (PDA, potato dextrose broth) Potato extract 4; Glucose 20; pH 5.6 Nutrient Bros (NB, Nutrient Bros) Peptone 5; Meat extract 3; pH 7.4 YM broth (Yeast-Malt Extract Bros) Yeast extract 3; Malt extract 3; Peptone 5; Glucose 10; pH 6.2 MRS broth, Man, Rogosa and Sharpe Bros Peptone 10; Meat extract 8; Yeast extract 4; Glucose 20; Sodium acetate 5; Triammonium citrate 2; Magnesium sulfide 0.2; Manganese sulfide 0.05; Dibasic potassium phosphate 2; Polysorbate 80 1; pH 6.2

The microbial agent of the present invention first has a ratio of 0.5 to 2: 1 to 3: 1 to 3: 0.5 to 2, preferably 1: 2: 2: 1 by weight of vermiculite, wheat bran, soybean meal, and ganbanite. After obtaining the excipients mixed in the ratio, 30 to 60% by weight of the cell culture medium mixture obtained by culturing each microorganism according to the above-described method and 40 to 70% by weight, preferably 50% by weight of each of the excipients and then 8 to Obtained by grinding at low temperature drying at 30 to 45 ° C., preferably at 40 ° C. for 24 hours, preferably 15 hours. When the cells are pulverized with a grinder and mixed with an excipient and dried, the cells penetrate well into the pores of vermiculite or elvan, increasing stability, and promoting secondary culture occurring early in the drying process. In addition, soybean meal and bran act as a nutrient source of the strain to promote the growth of bacteria.

In addition, the present invention as a preferred aspect of the Mucor sp. ( Mucor ) sp. A fungus deposited in the Korean spawn association showing the effect of increasing the productivity of the livestock and at the same time reduce the odor caused by livestock manure. KFCC-11239, Aspergillus oryzae KFCC-60241, Bacillus licheniformis KCTC 1030, Bacillus subtilis KCTC-1666, Lactobacillus acidophilus ) ATCC 4356, Saccharomyces cerevisiae ATCC 9763 and a method for producing the same.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in detail, and the scope of the present invention is not limited by these examples.

<Example 1>

Microbial Isolation and Identification

In order to select the optimal medium for culturing the microorganisms of the novel muker of the present invention was cultured by Chapec yeast agar (CYA) medium, malt extract agar (MEA) medium, glycerol 25% nitrate agar (G25N) medium . The composition of each medium was as follows.

(1) CYA (Czapek Yeast Agar): Chapek East Baby

NaNO ₃ 3.0g, K ₂ HPO ₄ 1.0g, KCl 0.5g, MgSO ₄ · 7H ₂ O 0.5g, FeSO ₄ · 7H ₂ O 0.1g, yeast extract 5.0g, saccharose 30g, agar 15g, distilled water 1L, pH 7.1

(2) Malt Extract Agar (MAL): malt extract agar

Malt extract 20g, peptone 1g, glucose 20g, agar 15g, distilled water 1L, pH 5.6

(3) G25N (Glycerol 25% Nitrate Agar): Glycerol 25% Nitrate Agar

NaNO ₃ 3.0 g, K ₂ HPO ₄ 1.0 g, MgSO ₄ · 7H ₂ O 0.5 g, yeast extract 5.0 g, glycerol 25 g, agar 15 g, distilled water 1 L, pH 7.1

After culturing for 7 days, the diameter of the colonies was measured in cm. As a result, as shown in Table 2, the growth rate was different for each medium, showing the best growth in the MEA medium, 37 rather than 25 ° C., which is the general culture temperature of mold. It showed better growth at ℃.

Medium temperature CYA (1) MEA (2) G25N (3) 5 ℃ No growth No growth No growth 25 ℃ 3.7 cm 5.1 cm 1.7 cm 37 ℃ 4.2 cm 6.5 cm 2.5 cm

As a result of observing the colonies from the platelet of PDA (PDA, Potato Dextrose Agar) and cultivating the slides for the identification of the strain, the microscopic observation of the microorganisms showed that the mucosa, sporangiospores, without the septum is characteristic of the muker. Cold sporangium was identified, and no roots, swellings, or pathologic cells were observed. The fungus of the Muker genus belongs to the phycomycetes, which have no septum in the hyphae, and the ancestor fungi include the Rhizopus sp., Including the Muker genus, and the Absidia sp. Among these, the fungus in the muker is divided into spore cyst disease upright in mycelium and elongate, not branched and branched. The strain of the present invention appears as a non-branched strain, and forms spores at the ends thereof. Unlike Rhizopus sp., The strain of the present invention was identified as a fungus of Muker because it did not form rhizome and creep.

Item Muker strain Isolated strain Hypha + + Septa － － Ascospore － － Sporangiospore + + Foot cell － － Rhizoid － － Storlon － － Zygospore + + Sporangium + +

Therefore, the novel isolated strain of the present invention was confirmed to be a muker genus of ancestor fungi (Phycomycetes). The isolated strain was deposited on November 23, 2000, with the accession number KFCC-11239 to the Korean spawn association.

The results of observing the morphology of the isolated strain of the present invention under a general optical microscope at a magnification of 600 times are shown in Table 4.

Item Characteristic Sporangiospore Diameter: 5.2 ~ 7.5 ㎛ Round Sporangium Size: 50.1 to 97.1 µm

<Example 2>

Preparation of Microbial Agents

Mucus KFCC-11239 and Aspergillus aurise KFCC-60241 were inoculated in 50 ml of potato dextrose broth (PDB) with 1 platinum of the preserved strain in potato dextrose agar (PDA) medium and 180 rpm for 2 days at 37 ° C. After incubation in a 5L jar-fermentor (3) potato culture in a medium containing 0.3% by weight of 3L, 2% by weight of glucose in 2L aerobic culture for 2 days at 37 ℃, 200rpm.

Bacillus licheniformis KCTC 1030 and Bacillus subtilis KCTC-1666 were inoculated into 50 ml of nutrient broth (NB) by taking 1 platinum of the strain preserved in nutrient agar (NA) medium at 180 rpm for 12 hours at 37 ° C. After incubation, the culture was exhaled for 15 hours at 37 ° C. and 180 rpm in a 3 L medium containing 1 wt% soybean meal and 2 wt% molasses in a 5 L jar-fermenter.

Lactobacillus Escidophilus ATCC 4356 was inoculated in 50 ml of MRS broth, which was preserved in MRS agar, inoculated in 50 ml of MRS broth, and incubated at 120 rpm for 15 hours at 37 ° C. -The cells were incubated for 15 hours at 37 ° C. and 120 rpm in a 3L medium containing 0.5 wt% yeast extract, 0.7 wt% glucose, and 0.2 wt% sodium acetate.

ATCC 9763 in Saccharomyces cerevisiae was inoculated in 50 ml of YM broth in a strain preserved in YM agar, inoculated in 50 ml of YM broth, and incubated at 120 rpm for 15 hours at 37 ° C. Inoculated in 3 L medium containing 1% by weight, 0.5% molasses, 1% glucose by incubation at 37 ℃ for 24 hours.

Table 5 shows the results compared with the optimum medium used in the laboratory and spawn culture to determine the mass culture conditions of each strain. In Comparative Example, the optimum medium for seedlings in Table 1 was used. In the microorganisms of the genus Muker and Bacillus, the growth of the comparative example was excellent up to the log phase, but there was no difference in the harvesting time after reaching the stationary phase. In the microorganisms of the genus Lactobacillus and Saccharomyces, there was no difference in the growth of bacteria in the log phase, so there was no difference in the usability of the medium by the microorganisms. It was found to be a condition. Therefore, it is possible to produce a microbial agent with a high bacterial count at a low cost when cultivating a large amount of the medium according to the present invention.

Comparison of Bacterial Growth between Optimal and Mass Culture Medium Item Incubation time (h) Number of bacteria (CFU / ml) Incubation time (h) Number of bacteria (CFU / ml) mold Comparative example 12 7.5 × 10 ⁴ 24 3.5 × 10 ⁷ Example 8.2 × 10 ⁴ 3.3 × 10 ⁷ Bacillus Comparative example 4 1.4 × 10 ⁶ 10 2.2 × 10 ⁷ Example 5.2 × 10 ⁶ 2.4 × 10 ⁷ Lactobacillus Comparative example 8 7.0 × 10 ⁵ 15 2.7 × 10 ⁸ Example 6.5 × 10 ⁵ 1.1 × 10 ⁸ leaven Comparative example 8 3.5 × 10 ⁵ 24 8.5 × 10 ⁷ Example 3.5 × 10 ⁵ 7.5 × 10 ⁷

Excipients mixing vermiculite, bran, soybean meal, and ganban stone in a ratio of 1: 2: 2: 1 by weight and liquid culture medium cultured under the above conditions in a ratio of 1: 1 by weight, Drying at low temperature at 40 ° C. for 24 hours was pulverized to obtain a microbial agent. Thus obtained by mixing and pulverizing the antimicrobial agent of 1% by weight in sterile physiological saline in order to determine the number of bacteria of the antimicrobial agent 10 in multiples serially diluted to a result of the smeared and cultured on each of the agar medium, myukeo in mold 1.0 × 10 ⁷ CFU / g, Aspergillus duckling 1.1 × 10 ⁷ CFU / g, Bacillus genus 4.0 × 10 ⁸ CFU / g, Lactobacillus genus 1.0 × 10 ⁸ CFU / g, Saccharomyces genus 3.0 × 10 ⁷ CFU / g The result was a good microbial agent containing a large number of microorganisms.

<Example 3>

Dairy cow salary test

Forty-five Holstein cows of similar milk yield and fertility were selected and divided into 15 groups each without additives and additions (muker genus and complex microbial agent), and the microorganisms in muker and the microbial agent for feed addition prepared according to Example 2, respectively. Mixing 0.2% by weight in 50 days to add the addition was the result shown in Table 6.

division No furniture Addition Muker Complex microbial agent Test head 15 15 15 Production flow rate (kg / day) Before the test 15.8 15.7 15.9 Average during the test 15.3 16.2 16.5 Somatic cell count (10 ³ / ml) Before the test 532.5 551.3 520.8 Average during the test 442.4 302.3 230.5 Mastitis Infection 6 2 2

In the above results, the amount of oil produced was slightly lower than before the test, but in the case of the added group of muker and the group of added microorganisms including the microorganism of the group of muker, the amount of oil was significantly increased. In the somatic cell counts, mucus added and mixed microbial supplements were higher in somatic cell counts. The number of cows infected with mastitis also showed a significantly lower result than the group without addition. As a result, it could be seen that the cow's productivity alone was improved by only feeding the fungus in the muker, and the administration of complex microorganisms including the fungus in the muker showed an effect of further improving the productivity. These results are believed to be due to the effects of the growth of the fibrinolytic bacteria activity and fermentation in the rumen according to the addition of the microbial agent and the effect of supplying unknown growth factors and improving the immune capacity.

<Example 4>

Broiler pay test

The results of feeding the broiler (avatar) broiler cockerel by mixing 0.2 micro% by weight of the microorganisms and the microbial agent prepared according to Example 2 for 25 days from 3 days to 28 days of age were shown in Table 7 below. . After completion of the experiment, the broiler cockerel was carcassed, and the contents of the ileum were sampled and serially diluted to 10-fold. After lactic acid bacteria were plated in MRS medium and E. coli group was incubated at 37 ° C. for 48 hours. The number of colonies was counted and measured.

division No furniture Addition Muker Complex microbial agent Breeding head 60 60 60 Feed intake (g / mari) 80.1 79.1 78.9 Daily weight gain (g / mari) 56.4 60.7 61.5 Feed demand rate (feed intake / weight gain) 1.42 1.30 1.28 Enteric Lactobacillus Count (CFU / g) 2.1 × 10 ⁷ 2.3 × 10 ⁷ 3.1 × 10 ⁷ Intestinal coliform count (CFU / g) 3.3 × 10 ⁷ 7.2 × 10 ⁶ 5.5 × 10 ⁶ % Mortality 5 2 2

As shown in the above results, the daily microbial weight gain and feed requirement were improved by the feeding of the microorganisms in the muker alone, the number of enteric lactic acid bacteria increased slightly, and the number of E. coli bacteria decreased considerably, which also helped to stabilize the intestinal microflora. In addition, the case of using a microorganism containing a microorganism in the muker showed a better feeding effect than the case of feeding the microorganism in the muker alone.

Example 5

Pig salary test

The microbial agent prepared according to Example 2 was mixed and fed to a pig feed at a ratio of 0.2% by weight, and the results are shown in Table 8 below. This test was carried out until the average weight of pigs in the mammalian body reached 90kg, and the mammalian group was fed a mixture of 0.2% by weight when feeding substitute milk.

division No furniture Addition Muker Complex microbial agent Breeding head 40 40 40 Feed intake (g / mari) 1,825 1,712 1,699 Daily weight gain (g / mari) 558 633 630 Feed demand rate (feed intake / weight gain) 3.27 2.70 2.65 Hari frequency * 2.08 2.78 2.88 % Mortality 5 2 2

* Normal side: 3, Yanbian: 2, Thin side: 1, Waterside: 0

As a result, when feeding the microorganisms in the muker and complex microbial agent according to the present invention to pigs can be expected to have a clear effect in terms of weight gain and feed rate improvement, it was found that the stool state is very good, especially low hari occurrence frequency.

<Example 6>

Deodorization test of pig manure

The microbial agent obtained according to the method of Example 2 was added to 0.5% by weight of the pig manure mixture and fermented for 4 days to determine the odor removal ability. As shown in Table 9, hydrogen sulfide, sulfur dioxide, ammonia, It was found that the volatile fatty acid content was greatly reduced. Odor incidence was examined by the sensory method. The sensory test is a method in which 10 inspectors are allowed to smell different treated manure and then calculate the average value based on the degree of odor occurrence.

Item Addition No furniture Early 4 days later % Removal Odor level * Early 4 days later % Removal Odor level * Ammonia in Manure (ppm) 112 38 66.1 4.6 112 61 45.5 7.0 Hydrogen sulfide in manure (ppm) 119 17 85.7 119 49 58.8 Volatile Fatty Acids in Manure (mM) 56 12 78.6 56 33 41.1

* Odor: almost 1-2 odors, 3-5 moderate, 6-8 severe odors, 9-10 very bad odors

As described above, when feeding the microorganisms comprising the mucker microorganism and the muker microorganism prepared by the present invention as livestock feed additives to the livestock, it is possible to expect a marked improvement in the productivity of the livestock and a reduction in the generation of odors of manure. It is a useful invention that can greatly contribute to.

Claims (10)

Enrichment of livestock consisting of 30 to 60% by weight of a cell culture mixture consisting of a mixture with the strains of the genus Muker fungus, Aspergillus, Bacillus, Lactobacillus and Saccharomyces and microorganism stabilized excipients 40 to 70% And microbial agent for the addition of livestock feed effective to reduce the occurrence of odor of livestock manure. The microorganism according to claim 1, wherein the fungus in the muker is fungus KFCC-11239. The genus Aspergillus strain is Aspergillus oryzae KFCC-60241, and the strain Bacillus is Bacillus licheniformis KCTC 1030 and Bacillus subtilis KCTC. Lactobacillus acidophilus ATCC 4356, and Saccharomyces strain Saccharomyces cerevisiae ATCC 9763. The strain is Lactobacillus sp. The microbial agent according to claim 1, wherein the microbial stabilizing excipient comprises vermiculite, bran, soybean meal and elvan. 1 to 3% by weight of glucose and 0.1 to 0.5% by weight of potato powder are used as a culture medium for culturing the fungus of the genus Muker and the genus Aspergillus; 0.1 to 1.0% by weight of yeast extract, 0.3 to 1.0% by weight of glucose and 0.1 to 0.5% by weight of sodium acetate are used as a medium for culturing the genus Lactobacillus; 0.5 to 3% by weight of soybean meal and 1 to 5% by weight of molasses are used as a medium for culturing Bacillus microorganisms; Culturing each microorganism using 0.5-3% by weight of soybean meal, 0.1-2.0% by molasses and 0.5-3% by weight of glucose as a yeast culture medium; Mixing said microbial culture mixture with a microbial stabilized excipient comprising vermiculite, bran, soybean meal and elvan; And low temperature drying and pulverizing the mixture. The method according to claim 5, wherein the fungus in the muker is fungus KFCC-11239. 6. The genus Aspergillus strain is Aspergillus oryzae KFCC-60241, and the strain Bacillus is Bacillus licheniformis KCTC 1030 and Bacillus subtilis KCTC. Lactobacillus acidophilus ATCC 4356, Saccharomyces strain Saccharomyces cerevisiae ATCC 9763 . Culturing the microorganisms using 1 to 3% by weight of glucose and 0.1 to 0.5% by weight of potato powder as a medium for culturing fungi in muker; Mixing said microbial culture mixture with a microbial stabilized excipient comprising vermiculite, bran, soybean meal and elvan; And low temperature drying and pulverizing the mixture. 9. The method according to claim 8, wherein the fungus of the muker is fungus KFCC-11239. Muker fungus KFCC-11239.