CN104161207B - The feed additive of a kind of anti-duck bacterial disease, preparation method and application - Google Patents
The feed additive of a kind of anti-duck bacterial disease, preparation method and application Download PDFInfo
- Publication number
- CN104161207B CN104161207B CN201310304153.0A CN201310304153A CN104161207B CN 104161207 B CN104161207 B CN 104161207B CN 201310304153 A CN201310304153 A CN 201310304153A CN 104161207 B CN104161207 B CN 104161207B
- Authority
- CN
- China
- Prior art keywords
- bacterial disease
- duck
- yolk antibody
- feed additive
- parts
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Abstract
The invention discloses the feed additive of a kind of anti-duck bacterial disease, preparation method and application, this feed additive includes the component of following parts by weight: anti-duck bacterial disease yolk antibody lyophilized powder 25 parts, astragalus polysaccharides 5 10 parts, lycium barbarum polysaccharide 10 15 parts, Angelica Polysaccharide 10 20 parts, Herba Houttuyniae 9.5 16 parts, Rhizoma Coptidis 5 10 parts, flower of poplar 10 20 parts.The anti-duck bacterial disease feed additive of the present invention is anti-duck bacterial disease yolk antibody lyophilized powder and astragalus polysaccharides, lycium barbarum polysaccharide, Angelica Polysaccharide, Herba Houttuyniae, Rhizoma Coptidis, flower of poplar is compounding forms, it is added in daily feedstuff, immunity of organisms and resistance against diseases can be improved.Test result indicate that, after adding the feed additive of the present invention, the effect of preventing duck bacterial disease significantly improves, hence it is evident that be better than individually adding the effect of yolk antibody lyophilized powder or Chinese medicine composition.
Description
Technical field
The present invention relates to the feed additive of a kind of anti-duck bacterial disease, also relate to this feed additive preparation method and
Application, belongs to field of fodder.
Background technology
Along with development and the raising of living standards of the people of society, more and more people turn one's attention to food-safety problem.Duck is for I
Accounting for share in state's aquaculture heavier, the annual amount of delivering for sale nearly 2,000,000,000, meat yield reaches nearly 3,000,000 tons, and annual return reaches
To more than 30 hundred million.But due to the restriction of rearing conditions, duck transmission of disease is the most universal.During duck culturing, commonly duck is thin
Bacterium property disease, wherein infectious serositis in duck is to endanger one of the most serious epidemic disease of duckling, and infectious serositis in duck is also called pest of duck
Bacillus pasteurii disease, new duck disease or duck septicemia, be that the one of the infringement duckling caused by Pasteurella anatipestipestifer is chronic or acute deteriorated blood
Sexually transmitted disease.This disease is widely distributed in all over the world, causes death, loses weight etc. and to cause huge economic damage to duck culturing industry
Lose.
The method of the most common preventing and treating duck bacterial disease is more, mainly uses Western medicine, immunoprophylaxis, herbal control.
Spreading unchecked of its Chinese medicine and western medicine use brings great diet hidden danger to duck product is edible, and drug residue has become as affects duck product peace
One of full principal element.Immunoprophylaxis early stage results is preferable, however it is necessary that and repeatedly inoculates, and troublesome poeration is relatively costly, holds
Easily cause duckling and produce stress；And vaccine requires higher for storage conditions, need cold preservation under the conditions of 2-8 DEG C, room temperature
Under the conditions of a period of time then lose effect.Chinese herbal feed additive is also one of current research direction, but due to Chinese herbal medicine
Kind more, identical Chinese crude drug is very big to the difference between the effects of different diseases of bird and livestock, and the immune effect that Chinese herbal medicine is to disease
Extremely limited, therefore its application also has certain limitation.Injection yolk antibody is also a kind of relatively conventional method, but
The immune effect of single injection yolk antibody sometimes can not meet actual demand.
Summary of the invention
It is an object of the invention to provide the feed additive of a kind of anti-duck bacterial disease, can effectively prevent the bacillary disease of multiple duck
Sick.
In order to realize object above, the technical solution adopted in the present invention is to provide the feed additive of a kind of anti-duck bacterial disease,
Described feed additive includes the component of following parts by weight: anti-duck bacterial disease yolk antibody lyophilized powder 2-5 part, the Radix Astragali are many
Sugar 5-10 part, lycium barbarum polysaccharide 10-15 part, Angelica Polysaccharide 10-20 part, Herba Houttuyniae 9.5-16 part, Rhizoma Coptidis 5-10 part, flower of poplar
10-20 part.
The feed additive of described anti-duck bacterial disease includes the component of following parts by weight: anti-duck bacterial disease yolk antibody
Lyophilized powder 2-5 part, astragalus polysaccharides 5-10 part, lycium barbarum polysaccharide 10-15 part, Angelica Polysaccharide 10-20 part, Herba Houttuyniae 10-15 part,
Rhizoma Coptidis 5-10 part, flower of poplar 10-20 part.
Described anti-duck bacterial disease yolk antibody lyophilized powder includes the component of following parts by weight: anti-duck bacterial disease yolk resists
Body 93-96 part, preservative 0.01-0.02 part, inactivator 0.2-0.3 part, vitamin C 0.5-1 part, polyvinylpyrrolidone 0.5-1
Part, glycine 0.5-1 part, sorbitol 1-2 part, glucose 1-2 part.
Described inactivator is formaldehyde.
Described preservative is thimerosal.
The present invention also aims to provide the preparation method of the feed additive of a kind of anti-duck bacterial disease.
The technical solution adopted in the present invention is to provide the preparation method of the feed additive of a kind of anti-duck bacterial disease, including with
Lower step:
1) duck bacterial disease pathogenic bacterium are bred and inactivated being prepared as vaccine, immune health chicken group prepares high-immunity egg, by high-immunity egg
Anti-duck bacterial disease yolk antibody lyophilized powder is obtained through sterilization, acidification, centrifugal treating, preparation, lyophilizing；
2) anti-duck bacterial disease yolk antibody lyophilized powder mixed with remaining component and get final product.
The preparation method of the feed additive of described anti-duck bacterial disease, specifically includes following steps:
1) duck bacterial disease pathogenic bacterium is bred and inactivate it is prepared as vaccine, at interval of 7 days, healthy laying hen group is carried out immunity,
Immunity three times, start after immunity to detect antibody titer for the second time altogether, when antibody titer reaches 1:256, collect high-immunity egg, will
The high-immunity egg collected carries out clearing up, sterilizing, and dries standby；
2) separate high-immunity egg yolk, add isopyknic sterilized water, be sufficiently stirred for, obtain yolk antibody solution；By yolk antibody
Solution is put into 60-65 DEG C of water-bath and is uniformly heated 30min, is subsequently adding pH=4.9-5.2, temperature is that the acidifying of 4-6 DEG C is water-soluble
Liquid, acidified aqueous solution addition is 6 times of yolk antibody liquor capacity, mixing, staticly settles, takes supernatant, 15000rpm
Centrifugal 20min, obtains the supernatant containing yolk antibody；
3) with the filter membrane of 0.45 μm to step 2) gained supernatant liquid filtering, the molecular weight that the dams macromolecular substances more than 200KD,
Again by filtrate through 0.22 μm membrane filtration, reserved filtrate；
4) filtrate is concentrated with ultrafiltration apparatus, make yolk antibody solution be concentrated into 2.5mL/500 plumage part；
5) in step 4) gained yolk antibody solution add inactivator, preservative, vitamin C, polyvinylpyrrolidone,
Glycine, sorbitol, glucose, and the most freezing under the conditions of 80 DEG C；
6) the yolk antibody solution of step 5) pre-cooling is proceeded to freeze dryer and prepare anti-duck bacterial disease yolk antibody lyophilized powder；
7) by anti-duck bacterial disease yolk antibody lyophilized powder and astragalus polysaccharides, lycium barbarum polysaccharide, Angelica Polysaccharide, Herba Houttuyniae, Rhizoma Coptidis,
Flower of poplar mixes and get final product.
The present invention also aims to the application providing a kind of feed additive in the feedstuff preparing anti-duck bacterial disease.
The technical solution adopted in the present invention also resides in a kind of feed additive of offer in the feedstuff preparing anti-duck bacterial disease
Application, the addition of described feed additive is the 3-7% of feedstuff gross weight.
Duck bacterial disease of the present invention is common duck bacterial disease, such as infectious serositis in duck.
Yolk antibody is a kind of immunoglobulin, the one produced by the stimulation of external antigen due to body stress product, can
To combine with corresponding antigens, eliminate the cause of disease.
Immunity of the present invention use be healthy chicken group as immunization, reduce the probability of strain reversion, equally make antibody
Titer is rapidly achieved 1:256.Anti-duck bacterial disease yolk antibody and multiple auxiliary materials are compounded by the present invention, and by adjusting
The composition of adjuvant, control its consumption, substantially prolongs the holding time of yolk antibody, it is ensured that yolk antibody is when longer one section
Interior activity.
Polysaccharide has raising body immunity, the adaptation ability of enhancing body and resistivity, antibacterial, antibacterium, regulation machine
Body metabolism etc. act on.Wherein,
Astragalus polysaccharides is the principle active component of the Radix Astragali, and the Radix Astragali is sweet, temperature.Return lung, spleen channel.Benefiting qi and raising yang, strengthening superficial resistance to stop perspiration,
Inducing diuresis to remove edema, detoxification granulation promoting.Cure mainly the card of all Qi and blood deficiencies.Mainly act on kidney diuresis, for promoting DNA in cell
Synthesis, accelerates the effect of liver differentiation and proliferation, improves propagation and the enhancing of leukocyte inducement interferon ability of lymphocyte, indirectly
Improve and promote immunity of organisms function.
Lycium barbarum polysaccharide is the principle active component of Fructus Lycii, and Fructus Lycii is sweet, flat.Return liver, kidney, lung meridian.Nourishing the liver, moistens, lung moistening.
Cure mainly caused by liver and kidney deficiency, have a dizzy spell, the most unclear, soreness of the waist and knees, impotence and seminal emission, cough due to consumptive disease, taking fluids profusely of quenching one's thirst.Main work
For internal organs such as Liver and kidney, scalable body's immunity, can effectively suppress tumor growth and cell mutation, have slow down aging,
Anti-fatty liver, regulation blood fat and the effect of the aspect such as blood glucose, promotion hemopoietic function.
Angelica Polysaccharide is the principle active component of Radix Glycyrrhizae, and Radix Glycyrrhizae is sweet, flat.Return spleen, stomach, the heart, lung meridian, mainly act on spleen
Dirty, tonification gas, heat-clearing and toxic substances removing, expelling phlegm for arresting cough, relieving spasm to stop pain, coordinating the actions of various ingredients in a prescription.For weakness of the spleen and stomach, fatigue and weakness, cardiopalmus
Breathe hard, cough with copious phlegm, cushion toxicity.
Herba Houttuyniae, property is pungent, be slightly cold.Return lung, bladder, large intestine channel.Heat-clearing and toxic substances removing, removing pus and relieving carbuncle, inducing diuresis for treating stranguria syndrome, cure mainly lung
Carbuncle vomiting pus, lung-heat cough with asthma, throat moth, too fat to move sore, hemorrhoid, hematodiarrhoea, pyretic stranguria, edema, leukorrhagia, scabies.Have antibacterial,
Antiviral, enhancing immunity, the effect of diuresis.
Rhizoma Coptidis, property is bitter, cold.GUIXIN, liver, stomach, large intestine channel.Clearing away heat-fire, dampness, removing toxic substances.Cure mainly calentura heresy popular feeling warp
Height hot, irritated, delirium or the absurd row of intenseness of heat forcing blood tell nosebleed, the damp and hot feeling of stuffiness in chest, have loose bowels, dysentery, the vexed insomnia of hyperactivity of heart-fire,
Gastropyretic vomiting, rapid digestion of food and polyorexia, liver-fire conjunctival congestion and swelling pain, and pathopyretic ulcer, furunculosis septicemia, gingival swelling and pain, aphtha of the mouth and tongue,
Ear, hemorrhoidal bleeding, eczema, scald.There is antimicrobial and protozoacide, antiviral, antineoplastic action.
Flower of poplar, heat-clearing and toxic substances removing, removing dampness dysentery relieving.Cure mainly bacillary dysentery, enteritis.
Said herbal medicine has the effect of regulation immunity of organisms to body, protects internal organs while the anti-stress ability of enhancing body,
Enhance the yolk antibody direct effect to body.
The anti-duck bacterial disease feed additive of the present invention be anti-duck bacterial disease yolk antibody lyophilized powder and by astragalus polysaccharides,
The Chinese medicine compositions such as lycium barbarum polysaccharide, Angelica Polysaccharide, Herba Houttuyniae, Rhizoma Coptidis, flower of poplar are compounding to be formed, and has with strong points, antibody
Titer is high, the advantage that biological activity is good, it is possible to play the effect of effective preventing duck bacterial disease, and preserve at normal temperatures very
Remain to for a long time keep activity.The feed additive of the present invention has no side effect, and noresidue endangers, safely and effectively, thin for duck
Bacterium property disease, has good preventive effect in particular for infectious serositis in duck.The present invention is according to duck bacterial disease not
With using corresponding yolk antibody, coordinate the use of Chinese herbal medicine simultaneously, there is good preventive effect.
Anti-duck bacterial disease yolk antibody lyophilized powder and Chinese medicine composition are reasonably combined by the present invention, are added on daily feedstuff
In, immunity of organisms and resistance against diseases can be improved.Test result indicate that, after feeding the feed additive adding the present invention, young
The bacteria resistance disease ability of duck significantly improves, and the effect of its anti-duck bacterial disease is substantially better than individually adds yolk antibody lyophilizing
Powder or the effect of Chinese medicine composition.
Detailed description of the invention
Embodiment 1
The treating infective serositis of duck yolk antibody lyophilized powder of the present embodiment, including the component of following parts by weight: anti-duck infectious
Oromeningitis yolk antibody 93 parts, thimerosal 0.01 part, 0.3 part of formaldehyde, vitamin C 0.5 part, polyvinylpyrrolidone 1 part,
Glycine 1 part, sorbitol 1 part, glucose 2 parts.
The feed additive of the treating infective serositis of duck of the present embodiment, including the component of following parts by weight: anti-duck infectious slurry
Film inflammation yolk antibody lyophilized powder 4 parts, astragalus polysaccharides 5 parts, lycium barbarum polysaccharide 10 parts, Angelica Polysaccharide 20 parts, Herba Houttuyniae 10 parts,
Rhizoma Coptidis 10 parts, flower of poplar 15 parts.
The preparation of the treating infective serositis of duck yolk antibody of the present embodiment, comprises the following steps:
1) isolated strain prepares vaccine: infectious serositis in duck standard strain Listeria monocytogenes is bred and is inactivated and makes emulsifiable concentrate vaccine,
Standby；
2) immune health chicken group obtains egg: every 7 days to healthy chicken immunity once, altogether immunity three times, immune health chicken for the first time
Group inoculation inactivated vaccine 1.5mL, for the second time, immunity inoculation dosage 1 times for the third time, starts after immunity to detect antibody effect for the second time
Valency, when antibody titer reaches 1:256, collects high-immunity egg, carries out the high-immunity egg of collection clearing up, sterilizing, dry standby；
3) acidification: separate high-immunity egg yolk, add isopyknic sterilized water, be sufficiently stirred for, obtain yolk antibody solution；
Yolk antibody solution is put into 63 DEG C of water-baths and uniformly heats 30min, be subsequently adding pH=5, temperature is that the acidifying of 5 DEG C is water-soluble
Liquid, acidified aqueous solution addition is 6 times of yolk antibody liquor capacity, mixing, staticly settles, takes supernatant, 15000rpm,
Centrifugal 20min, stays supernatant；
4) with the filter membrane of 0.45 μm, step 3) gained supernatant liquid filtering, the molecular weight that dams are more than the macromolecular substances of 200KD,
Again by filtrate through 0.22 μm membrane filtration, reserved filtrate；
5) filtrate is concentrated with ultrafiltration apparatus, make yolk antibody solution be concentrated into 2.5mL/500 plumage part.
6) concentrate to step 5) gained yolk antibody solution adds formaldehyde, thimerosal, vitamin C, polyvinylpyrrolidone,
Glycine, sorbitol, glucose；
7) under the conditions of the yolk solution of step 6) being placed into-80 DEG C, fully freezing；
8) step 7) gained pre-cooling yolk antibody solution is proceeded to freeze dryer and prepare treating infective serositis of duck yolk antibody lyophilized powder；
9) by treating infective serositis of duck yolk antibody lyophilized powder and astragalus polysaccharides, lycium barbarum polysaccharide, Angelica Polysaccharide, Herba Houttuyniae, Huang
Even, flower of poplar mixes and get final product.
Embodiment 2
The treating infective serositis of duck yolk antibody lyophilized powder of the present embodiment, including the component of following parts by weight: anti-duck infectious
Oromeningitis yolk antibody 95 parts, thimerosal 0.02 part, 0.2 part of formaldehyde, vitamin C 1 part, polyvinylpyrrolidone 0.5 part,
Glycine 0.5 part, sorbitol 1.5 parts, glucose 1.5 parts.
The feed additive of the treating infective serositis of duck of the present embodiment, including the component of following parts by weight: anti-duck infectious slurry
Film inflammation yolk antibody lyophilized powder 5 parts, astragalus polysaccharides 8 parts, lycium barbarum polysaccharide 15 parts, Angelica Polysaccharide 10 parts, Herba Houttuyniae 15 parts,
Rhizoma Coptidis 5 parts, flower of poplar 10 parts.
The preparation method of the feed additive of the treating infective serositis of duck of the present embodiment is with embodiment 1, and difference is each group
The consumption divided is different.
Embodiment 3
The treating infective serositis of duck yolk antibody lyophilized powder of the present embodiment, including the component of following parts by weight: anti-duck infectious
Oromeningitis yolk antibody 96 parts, thimerosal 0.015 part, 0.25 part of formaldehyde, vitamin C 0.8 part, polyvinylpyrrolidone 0.7
Part, glycine 0.6 part, sorbitol 2 parts, glucose 1 part.
The feed additive of the treating infective serositis of duck of the present embodiment, including the component of following parts by weight: anti-duck infectious slurry
Film inflammation yolk antibody lyophilized powder 2 parts, astragalus polysaccharides 10 parts, lycium barbarum polysaccharide 13 parts, Angelica Polysaccharide 15 parts, Herba Houttuyniae 12 parts,
Rhizoma Coptidis 8 parts, flower of poplar 20 parts.
The preparation method of the feed additive of the treating infective serositis of duck of the present embodiment is with embodiment 1, and difference is each group
The consumption divided is different.
Comparative example 1
The feed additive of the treating infective serositis of duck of this comparative example, including the component of following parts by weight: anti-duck infectious slurry
Film inflammation yolk antibody 94 parts, thimerosal 0.015 part, 0.25 part of formaldehyde, vitamin C 0.8 part, polyvinylpyrrolidone 0.7
Part, glycine 0.6 part, sorbitol 2 parts, glucose 1 part.
Comparative example 2
The feed additive of the treating infective serositis of duck of this comparative example, including the component of following parts by weight: astragalus polysaccharides 8 parts,
Lycium barbarum polysaccharide 15 parts, Angelica Polysaccharide 10 parts, Herba Houttuyniae 15 parts, Rhizoma Coptidis 5 parts, flower of poplar 10 parts.
Experimental example 1
The quality testing of feed additive of the present invention
Experimental technique: the feed additive of the present invention is saved in 37 DEG C of incubators, preserves 24 months, 1 week respectively, 2 weeks,
January, February, April, August, December, 16 months, 20 months, after 24 months, by agar diffusion (AGP) method, measure each
The yolk antibody titer change of point in time sampling product, the results are shown in Table 1.
Table 1 room temperature places the change (project: infectious serositis) of lyophilized powder different time sections titer
| Group | 1 week | 2 weeks | January | February | April | August | December | 16 months | 20 months | 24 months |
| Embodiment 1 | 1:32 | 1:32 | 1:32 | 1:32 | 1:32 | 1:32 | 1:16 | 1:16 | 1:16 | 1:16 |
| Embodiment 2 | 1:32 | 1:32 | 1:32 | 1:32 | 1:32 | 1:16 | 1:16 | 1:16 | 1:16 | 1:16 |
| Embodiment 3 | 1:32 | 1:32 | 1:32 | 1:32 | 1:32 | 1:16 | 1:16 | 1:16 | 1:16 | 1:16 |
Yolk antibody quality testing:
[mycoplasma inspection] is carried out by " Chinese veterinary pharmacopoeia ", grows without mycoplasma.
[external source bacteriologic test] is carried out by " Chinese veterinary pharmacopoeia ", meets regulation.
[safety verification], with 7 age in days duckling 5, intramuscular injection 5 plumage part, is observed 2 weeks, should all be good for and live, and injection site does not has
There is pathological changes.
[shelf-life], according to room temperature bioactivity experimental result, can deposit 2 years.
Experimental example 2
The effect analysis of the feed additive treating infective serositis of duck of the present invention
The normal diet of this experimental example is commercially available conventional feed.
Experimental technique: choosing 7 age in days duckling 700, wherein 100 be only used as counteracting toxic substances group isolated rearing, 100 are only used as blank
Group isolated rearing, 200 are only used as matched group isolated rearing, and 300 are only used as test group isolated rearing.Counteracting toxic substances group and blank group are all raised
Feeding normal diet, matched group is divided into 2 groups, often group 100, feeds addition 7% comparative example 1-2 feed additive+general respectively
Logical feedstuff, test group is divided into 3 groups, often group 100, isolated rearing, feeds addition 7% embodiment 1-3 feedstuff respectively and adds
Agent+normal diet.Feeding manner uses free choice feeding, freely drinks water, after 24h, takes test group, matched group and the young bird of counteracting toxic substances group
Infectious serositis liquid after duck intramuscular injection inoculation dilution, records 120h disease condition, and result is as shown in table 2.
The effect analysis of the feed additive treating infective serositis of duck of table 2 embodiment 1-3
From Table 2, it can be seen that after with the addition of the feed additive of the present invention, duck bacteria resistance disease ability significantly improves, anti-
The effect of duck bacterial disease is also significantly better than individually adds yolk antibody lyophilized powder or the effect of Chinese medicine composition.
Claims (9)
1. the feed additive of an anti-duck bacterial disease, it is characterised in that described feed additive is by following weight portion
The component composition of number: anti-duck bacterial disease yolk antibody lyophilized powder 2-5 part, astragalus polysaccharides 5-10 part, lycium barbarum polysaccharide 10-15
Part, Angelica Polysaccharide 10-20 part, Herba Houttuyniae 9.5-16 part, Rhizoma Coptidis 5-10 part, flower of poplar 10-20 part.
The feed additive of a kind of anti-duck bacterial disease the most according to claim 1, it is characterised in that described in raise
Feed additives is made up of the component of following parts by weight: anti-duck bacterial disease yolk antibody lyophilized powder 2-5 part, astragalus polysaccharides
5-10 part, lycium barbarum polysaccharide 10-15 part, Angelica Polysaccharide 10-20 part, Herba Houttuyniae 10-15 part, Rhizoma Coptidis 5-10 part, flower of poplar
10-20 part.
The feed additive of a kind of anti-duck bacterial disease the most according to claim 1, it is characterised in that described anti-
Duck bacterial disease yolk antibody lyophilized powder includes the component of following parts by weight: anti-duck bacterial disease yolk antibody 93-96
Part, preservative 0.01-0.02 part, inactivator 0.2-0.3 part, vitamin C 0.5-1 part, polyvinylpyrrolidone 0.5-1 part,
Glycine 0.5-1 part, sorbitol 1-2 part, glucose 1-2 part.
The feed additive of a kind of anti-duck bacterial disease the most according to claim 3, it is characterised in that described in go out
Agent of living is formaldehyde.
The feed additive of a kind of anti-duck bacterial disease the most according to claim 3, it is characterised in that described anti-
Rotten agent is thimerosal.
6. the preparation method of the feed additive of an anti-duck bacterial disease as claimed in claim 1, it is characterised in that
Comprise the following steps:
1) duck bacterial disease pathogenic bacterium are bred and inactivated being prepared as vaccine, immune health chicken group prepares high-immunity egg, by height
Exempt from egg and obtain anti-duck bacterial disease yolk antibody lyophilized powder through sterilization, acidification, centrifugal treating, preparation, lyophilizing；
2) anti-duck bacterial disease yolk antibody lyophilized powder mixed with remaining component and get final product.
The preparation method of the feed additive of a kind of anti-duck bacterial disease the most according to claim 6, its feature exists
In, specifically include following steps:
1) duck bacterial disease pathogenic bacterium are bred and inactivate it is prepared as vaccine, at interval of 7 days, healthy laying hen group is carried out
Immunity, immunity three times, start after immunity to detect antibody titer for the second time altogether, when antibody titer reaches 1:256, collect
High-immunity egg, carries out the high-immunity egg of collection clearing up, sterilizing, dries standby；
2) separate high-immunity egg yolk, add isopyknic sterilized water, be sufficiently stirred for, obtain yolk antibody solution；By yolk
Antibody-solutions is put into 60-65 DEG C of water-bath and is uniformly heated 30min, is subsequently adding pH=4.9-5.2, temperature is the acid of 4-6 DEG C
Changing aqueous solution, acidified aqueous solution addition is 6 times of yolk antibody liquor capacity, mixing, staticly settles, takes supernatant,
15000rpm is centrifuged 20min, obtains the supernatant containing yolk antibody；
3) with the filter membrane of 0.45 μm to step 2) gained supernatant liquid filtering, the molecular weight that the dams macromolecular complex more than 200KD
Matter, then by filtrate through 0.22 μm membrane filtration, reserved filtrate；
4) filtrate is concentrated with ultrafiltration apparatus, make yolk antibody solution be concentrated into 2.5mL/500 plumage part；
5) to step 4) gained yolk antibody solution adds inactivator, preservative, vitamin C, polyvinylpyrrolidone,
Glycine, sorbitol, glucose, and the most freezing under the conditions of 80 DEG C；
6) by step 5) the yolk antibody solution of pre-cooling proceeds to freeze dryer and prepares anti-duck bacterial disease yolk antibody lyophilized powder；
7) by anti-duck bacterial disease yolk antibody lyophilized powder and astragalus polysaccharides, lycium barbarum polysaccharide, Angelica Polysaccharide, Herba Houttuyniae,
Rhizoma Coptidis, flower of poplar mix and get final product.
8. a feed additive as claimed in claim 1 application in the feedstuff preparing anti-duck bacterial disease.
Application the most according to claim 8, it is characterised in that the addition of described feed additive is that feedstuff is total
The 3-7% of weight.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310304153.0A CN104161207B (en) | 2013-07-18 | 2013-07-18 | The feed additive of a kind of anti-duck bacterial disease, preparation method and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310304153.0A CN104161207B (en) | 2013-07-18 | 2013-07-18 | The feed additive of a kind of anti-duck bacterial disease, preparation method and application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104161207A CN104161207A (en) | 2014-11-26 |
| CN104161207B true CN104161207B (en) | 2016-08-17 |
Family
ID=51905318
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310304153.0A Expired - Fee Related CN104161207B (en) | 2013-07-18 | 2013-07-18 | The feed additive of a kind of anti-duck bacterial disease, preparation method and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104161207B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105582037A (en) * | 2016-01-27 | 2016-05-18 | 成都乾坤动物药业有限公司 | Medicine composition for treating bacterial diseases of poultry |
| CN106879858A (en) * | 2016-12-30 | 2017-06-23 | 新昌县拜特夫农业科技有限公司 | A kind of feed addictive of anti-duck bacteriosis |
| CN115837049A (en) * | 2022-11-14 | 2023-03-24 | 江苏南京农大动物药业有限公司 | Compound poplar flower oral liquid for preventing and treating livestock and poultry diarrhea and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1352887A (en) * | 2001-11-24 | 2002-06-12 | 宁夏农林科学院畜牧兽医研究所 | Feed additive made of Chinese-medicinal herbs |
| CN1385442A (en) * | 2002-05-17 | 2002-12-18 | 重庆和润实业(集团)有限公司 | Compound yolk antibody capable of resisting fowl bacterial blight and its preparation and use |
| CN101475639A (en) * | 2008-08-19 | 2009-07-08 | 福州大北农生物技术有限公司 | Mixed yolk antibody for preventing and treating duck infectious serositis and preparation thereof |
| CN102716484A (en) * | 2012-05-31 | 2012-10-10 | 郑州后羿制药有限公司 | Duck virus hepatitis yolk antibody freeze-dried powder and preparation method thereof |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101391102A (en) * | 2007-09-23 | 2009-03-25 | 方希修 | Production technique of immunoglobulin compounding agent capable of preventing and treating poultry respiratory disease |
| CN102302778A (en) * | 2011-09-26 | 2012-01-04 | 方希修 | Preparation method and application of traditional Chinese medicinal immunoglobulin compound preparation |
| CN102584994A (en) * | 2012-03-26 | 2012-07-18 | 广东紫金正天药业有限公司 | Egg yolk antibody for curing colibacillosis and preparation method thereof |
-
2013
- 2013-07-18 CN CN201310304153.0A patent/CN104161207B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1352887A (en) * | 2001-11-24 | 2002-06-12 | 宁夏农林科学院畜牧兽医研究所 | Feed additive made of Chinese-medicinal herbs |
| CN1385442A (en) * | 2002-05-17 | 2002-12-18 | 重庆和润实业(集团)有限公司 | Compound yolk antibody capable of resisting fowl bacterial blight and its preparation and use |
| CN101475639A (en) * | 2008-08-19 | 2009-07-08 | 福州大北农生物技术有限公司 | Mixed yolk antibody for preventing and treating duck infectious serositis and preparation thereof |
| CN102716484A (en) * | 2012-05-31 | 2012-10-10 | 郑州后羿制药有限公司 | Duck virus hepatitis yolk antibody freeze-dried powder and preparation method thereof |
Non-Patent Citations (4)
| Title |
|---|
| 中草药及其有效成分体外抗菌抗病毒研究进展;傅文栋;《中兽医医药杂志》;20061231(第05期);66-67 * |
| 植物提取物饲料添加剂开发应用的影响因素及对策;刘学剑;《饲料工业》;20071231;第28卷(第16期);59-61 * |
| 生物活性物质对家禽免疫的影响;李振;《中国饲料》;20040405(第04期);23-26 * |
| 鸭病毒性肝炎卵黄抗体的作用及应用;赵风立等;《山东畜牧兽医》;20101231;第31卷(第10期);68-69 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104161207A (en) | 2014-11-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104161181B (en) | The feed additive of a kind of anti-pig bacterial disease, preparation method and application | |
| CN102845637B (en) | Chicken feed capable of preventing and treating avian influenza and Chinese herbal additive used therein | |
| CN104012807B (en) | A kind of livestock and poultry antiviral B B-complex premixed feed preparation and preparation method and application thereof | |
| CN104161180B (en) | The feed additive of a kind of anti-porcine viral diseases, preparation method and application | |
| CN103598426B (en) | For improving feed addictive and the feed of growth performance of chicks | |
| CN104208673B (en) | A kind of fowl antiviral composition, lyophilized powder, preparation method and application | |
| CN104982751A (en) | Rice bran type piglet mixed feed and preparation method thereof | |
| CN103719623A (en) | Traditional Chinese medicine feed additive capable of preventing or relieving stress syndrome of weaned pigs and preparation method of feed additive | |
| CN104161208B (en) | Feed additive for preventing chicken bacterial diseases, preparation method and application thereof | |
| CN104161207B (en) | The feed additive of a kind of anti-duck bacterial disease, preparation method and application | |
| KR102213743B1 (en) | A process and the composition for increasing immunity-boosting and the growth of animals comprising herbs | |
| KR102012751B1 (en) | Platycodon grandiflorum extract animal feed additive and animal feed | |
| CN104161179B (en) | The feed additive of a kind of anti-canine viral disease, preparation method and application | |
| CN112807348B (en) | Disease-resistant and growth-promoting traditional Chinese medicine compound, and preparation and application thereof | |
| CN103719595B (en) | Chinese herbal medicine additive for piglets and preparing method thereof | |
| CN104161183B (en) | The feed additive of a kind of anti-chicken viral diseases, preparation method and application | |
| CN104982755A (en) | Piglet mixed feed and preparation method thereof | |
| CN104208678A (en) | Veterinary antivirus composition, freeze-dried powder, preparation method and applications of the composition | |
| CN104161182B (en) | The feed additive of a kind of anti-goose viral disease, preparation method and application | |
| CN104162157B (en) | The compositions of a kind of anti-swine fever, feed additive, preparation method and application | |
| CN104208683B (en) | The pharmaceutical composition of a kind of anti-duck viral disease, lyophilized powder, preparation method and application | |
| CN104161178B (en) | The feed additive of a kind of anti-duck viral disease, preparation method and application | |
| CN105724831A (en) | Grouper feed and preparation method thereof | |
| CN105192430A (en) | Chicken feed additive for preventing chicken diseases | |
| CN104799014A (en) | Natural plant feed additive for promoting healthy development of digestive tract of weaned piglet and preparing method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160817 Termination date: 20170718 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |