CN104208673B - A kind of fowl antiviral composition, lyophilized powder, preparation method and application - Google Patents
A kind of fowl antiviral composition, lyophilized powder, preparation method and application Download PDFInfo
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Abstract
The invention discloses a kind of fowl antiviral composition, lyophilized powder, preparation method and application, belong to poultry field of feed additive technology.This fowl antiviral composition is mainly made up of the component of following parts by weight: fowl antiviral yolk antibody 90～100 parts, fowl antiviral transfer factor 1～2 parts, interferon-alpha 1～2 parts；Preferably, the component of following parts by weight is also included: astragalus polysaccharides 5～10 parts, Radix Angelicae Sinensis polysaccharide 5～10 parts, lycium barbarum polysaccharide 10～20 parts, Angelica Polysaccharide 10～30 parts.Fowl antiviral yolk antibody, fowl antiviral transfer factor, interferon-alpha are compounded by the present invention with Chinese medicine extract astragalus polysaccharides, Radix Angelicae Sinensis polysaccharide, lycium barbarum polysaccharide and Angelica Polysaccharide, immunity and the resistance against diseases of birds body can be effectively improved, avian viral diseases is had good prophylactic-therapeutic effect.Can preserve the most for a long time after being prepared into lyophilized powder, antibody titer is high, biological activity is good.
Description
Technical field
Present invention relates particularly to a kind of fowl antiviral composition, go back wader antiviral lyophilized powder, and this lyophilized powder simultaneously
Preparation method and application, belong to poultry fodder additive technology field.
Background technology
Along with development and the raising of living standards of the people of society, increasing people begins to focus on food-safety problem.At me
State, poultry industry scale expanding day.But due to the restriction of rearing conditions, poultry disease is propagated the most universal.Poultry is supported
Most commonly seen during growing is viral disease, mainly includes newcastle disease (NDV), infectious bronchitis of chicken, chicken
Infectious bursal disease, bird flu, infectious laryngotracheitis of chicken, Avian viral arthritis, chicken egg-decreasing syndrome etc..
At present, preventing and treating chicken viral diseases mainly uses Western medicine, immunoprophylaxis and herbal control.Wherein, the spreading unchecked of Western medicine
Using and bring great potential safety hazard to chicken meat product, drug residue has become as the principal element affecting Carnis Gallus domesticus safety.Connect
Planting immunity effect in early days preferable, however it is necessary that and repeatedly inoculate, troublesome poeration, relatively costly, easily causing chickling stress be anti-
Should, and vaccine is the harshest for the requirement of storage conditions, needs cold preservation under the conditions of 2～8 DEG C, during room temperature condition next section
Between then lose effect.Though Chinese herbal feed additive is one of current research direction, but the kind of Chinese herbal medicine is more, phase
Relatively big to the difference between the effects of different diseases of bird and livestock with medical material, and Chinese herbal medicine is extremely limited to the immunity raising of disease, therefore it should
With also having certain limitation.Injection yolk antibody is also a kind of relatively conventional method, but single injection yolk antibody
Immune effect sometimes can not meet and be actually needed.
Summary of the invention
It is an object of the invention to provide a kind of fowl antiviral composition.
Meanwhile, the present invention also provides for a kind of fowl antiviral lyophilized powder.
Furthermore, the present invention also provides for the preparation method of a kind of fowl antiviral lyophilized powder.
Finally, the present invention also provides for the application as feed additive of a kind of fowl antiviral lyophilized powder.
In order to realize object above, the technical solution adopted in the present invention is:
A kind of fowl antiviral composition, is mainly made up of the component of following parts by weight: fowl antiviral yolk antibody 90～
100 parts, fowl antiviral transfer factor 1～2 parts, alpha-interferon 1～2 parts.
Preferably, a kind of fowl antiviral composition, also include the component of following parts by weight: astragalus polysaccharides 5～10 parts,
Radix Angelicae Sinensis polysaccharide 5～10 parts, lycium barbarum polysaccharide 10～20 parts, Angelica Polysaccharide 10～30 parts.
A kind of fowl antiviral lyophilized powder, is mainly made up of the component of following parts by weight: fowl antiviral yolk antibody 90～
100 parts, fowl antiviral transfer factor 1～2 parts, alpha-interferon 1～2 parts, preservative 0.01～0.02 part, inactivator
0.2～0.3 part, heat-resisting lyophilized protecting agent 3～6 parts.
Preferably, a kind of fowl antiviral lyophilized powder, also include the component of following parts by weight: astragalus polysaccharides 5～10 parts,
Radix Angelicae Sinensis polysaccharide 5～10 parts, lycium barbarum polysaccharide 10～20 parts, Angelica Polysaccharide 10～30 parts.
Described fowl is identical with the viral species that antiviral yolk antibody is targeted with fowl antiviral transfer factor, as new in anti-chicken
City epidemic disease yolk antibody and anti-newcastle disease transfer factor, anti-infectious bronchitis of chicken yolk antibody and anti-avian infectious bronchus
Scorching transfer factor, anti-infectious bursal disease yolk antibody and anti-infectious bursal disease transfer factor, resist avian infectious
Laryngotracheitis yolk antibody and anti-infectious laryngotracheitis of chicken transfer factor, anti-duck plague yolk antibody and anti-duck plague transfer factor or
Anti-duck virus hepatitis yolk antibody and anti-duck viral hepatitis transfer factor etc..
Described preservative is thimerosal.
Described inactivator is formaldehyde.
Described heat-resisting lyophilized protecting agent is made up of the component of following parts by weight: vitamin C 0.5～1 part, vitamin e1～
2 parts, polyvinylpyrrolidone 1～2 parts, glycine 0.5～1 part.
Described fowl antiviral transfer factor can be commercial goods, it would however also be possible to employ following methods prepares:
(1) clean the pig spleen using avian viruses antigen immune to cross and/or thymus with the normal saline after sterilizing, go to its surface
Muscle, fat etc. are organized, then use normal saline flushing；
(2) pig spleen and/or thymus after processing crush, and add the PBS of 2 times of pre-coolings, use tissue homogenate instrument
Homogeneous homogenate；
(3) homogenate ultrasonication process 5～10min must be crushed liquid；
(4) broken liquid being adjusted to pH value is 5.3～5.7, and 5000～6000rpm are centrifuged 30min, collect supernatant；
(5) by supernatant micro-filtrate membrane filtration, the daltonian material of molecular cut off 7000, collect filtrate；
(6) filtrate being used degerming membrane filtration, obtain fowl antiviral transfer factor stock solution, 4 DEG C of preservations or lyophilizing are standby.
Described astragalus polysaccharides can be commercial goods, it is also possible to extract preparation by conventional method.Wherein the Radix Astragali containing Saponin,
The various trace elements such as sucrose, polysaccharide, several amino acids, folic acid and selenium, zinc, copper.Mainly act on kidney, have diuresis to make
With, the synthesis of DNA in cell, the differentiation and proliferation of acceleration liver can be promoted, and the propagation of lymphocyte can be improved, strengthen white
The ability of cell inducement interferon, thus indirectly improve and promote immunity of organisms function.
Described Radix Angelicae Sinensis polysaccharide can be commercial goods, it is also possible to extract preparation by conventional method.Wherein Radix Angelicae Sinensis Main Function
Yu Xin, liver device, can regulate QI and blood, enhancing human body immunity function, also have antitumaous effect.
Described lycium barbarum polysaccharide can be commercial goods, it is also possible to extract preparation by conventional method.Wherein Fructus Lycii Main Function
In the internal organs such as liver, kidney, body's immunity can be regulated, effectively suppression tumor growth and cell mutation, have slow down aging,
Anti-fatty liver, regulation blood fat and the effect such as blood glucose, promotion hemopoietic function.
Described Angelica Polysaccharide can be commercial goods, it is also possible to extract preparation by conventional method.Wherein Radix Glycyrrhizae Main Function
In spleen, energy tonification gas, heat-clearing and toxic substances removing, expelling phlegm for arresting cough, relieving spasm to stop pain, coordinating the actions of various ingredients in a prescription；Weary for weakness of the spleen and stomach, asthenia
Power, shortness of breath and palpitation, cough with copious phlegm etc., moreover it is possible to cushion toxicity.
A kind of fowl preparation method of antiviral lyophilized powder, comprises the following steps:
(1) isolated strain prepares vaccine: prepare emulsifiable concentrate vaccine after taking Strain propagation, inactivation standby；
(2) immune health chicken group prepares high-immunity egg: takes vaccine virus immunization healthy chicken flock prepared by step (1), works as antibody
When titer reaches 1:256, dry standby after collecting high-immunity egg cleaning, sterilization；
(3) high-immunity egg yolk is separated: take high-immunity egg separation yolk prepared by step (2), add isopyknic sterilized water,
It is stirred well to color turn white to obtain yolk solution, yolk solution is placed in 60～65 DEG C of water-baths heating 20～30min, then adds
Entering the pre-cooling acidifying water that pH value is 4.9～5.2, addition is 6～8 times of yolk solution volume, left at room temperature after mixing
Precipitation, takes supernatant centrifugation；
(4) take step (3) centrifugal after supernatant, filter, make the dry weight of fowl antiviral yolk antibody in filtrate be 90～
100 parts, concentrate, in terms of parts by weight, add in concentrated solution preservative 0.01～0.02 part, inactivator 0.2～0.3 part,
Heat-resisting lyophilized protecting agent 3～6 parts, pre-freeze after mixing, lyophilizing are standby；
(5) in terms of parts by weight, the dry powder of step (4) adds fowl antiviral transfer factor 1～2 parts, α-interference
Element 1～2 part and get final product.
In described step (2) every 7 days to healthy chicken flock immunity once, altogether immunity three times, after second time immunity, start inspection
Survey antibody titer.Preferably, immunity inoculation inactivated vaccine 1.5mL for the first time, for the second time, third time immunity increase by 1 times amount.
In described step (3), the rotating speed of centrifugation is 15000rpm, and centrifugation time is 20min.
In described step (4), filtration first uses the filter membrane of 0.45 μm, with the molecular cut off macromolecular complex more than 200KD
Matter, then use the membrane filtration of 0.22 μm.
The powder that in described step (5), the fowl antiviral transfer factor of addition obtains after being lyophilised process, it is also possible to
The concentrated solution of step (4) adds the transfer factor stock solution after converting with dry weight.
Preferably, a kind of fowl preparation method of antiviral lyophilized powder, in terms of parts by weight, in the dry powder of step (5)
Add astragalus polysaccharides 5～10 parts, Radix Angelicae Sinensis polysaccharide 5～10 parts, lycium barbarum polysaccharide 10～20 parts, Angelica Polysaccharide 10～30 parts.
Usage and consumption: intramuscular injection, containing fowl antiviral lyophilized powder 1g in every 100ml injection, 0.1ml/kg body weight, often
Day 2 times, it is used in conjunction 5.
A kind of fowl, comprises the following steps: by weight percentage as the application of feed additive with antiviral lyophilized powder, will
Fowl antiviral lyophilized powder adds in poultry fodder with the amount of 0.2～0.6%.
Beneficial effects of the present invention:
The fowl of the present invention with antiviral composition mainly by fowl antiviral yolk antibody, fowl antiviral transfer factor, α-dry
Disturbing element composition, wherein yolk antibody is a kind of immunoglobulin, is that the one produced by external antigen stimulation by body should
Swash product, can be combined with corresponding antigens, eliminate the cause of disease；Transfer factor carries the specific immunity letter of primed lymphocyte
Breath, it is possible to specific immunity information is presented to recipient lymphocytes, and the lymphocyte making receptor inactive is changed into specificity
Primed lymphocyte, thus excite the immunoreation that recipient cell mediates；Alpha-interferon can be made by cell surface receptor effect
Cell produces antiviral protein, the most also can strengthen natural killer cell (NK cell), macrophage and T lymphocyte
Vigor, thus play immunoregulation effect, and strengthen anti-virus ability.Above-mentioned three combination can be strengthened machine by the present invention
Body antiviral activity, is greatly improved attack and defense effect, has good prophylactic-therapeutic effect to avian viral diseases.
The present invention uses astragalus polysaccharides, Radix Angelicae Sinensis polysaccharide, lycium barbarum polysaccharide, Angelica Polysaccharide totally 4 kinds of polysaccharose substances, has raising
Immunity of organisms, enhancing body adaptive faculty and resistance etc. act on, moreover it is possible to antibacterial, antiviral, regulation organism metabolism etc..On
State 4 kinds of materials and cover the internal organs such as the heart of body, liver, spleen, lung, kidney, spleen, it is ensured that the balance fortune between body internal organs
Turn, stronger than the curative effect of single utilization, body internal organs function can not only be strengthened, moreover it is possible to strengthen body immunity and regulative mechanism,
Ensure body health.
The present invention is by many to fowl antiviral yolk antibody, fowl antiviral transfer factor, alpha-interferon and astragalus polysaccharides, Radix Angelicae Sinensis
Sugar, lycium barbarum polysaccharide, Angelica Polysaccharide are combined, and have the advantages such as with strong points, antibody titer is high, biological activity is good, it is possible to carry
The immunity of high body and resistance against diseases, effectively prevent avian viral diseases.Above-mentioned active component is protected with heat resistant freeze drying
Agent compounds, and can extend the lyophilized powder holding time at normal temperatures, it is ensured that antibody titer and biological activity, by it by a certain percentage
Add in feed formula, can prevent and treat avian viral diseases, improve immunity of organisms and survival rate, easy to use.
The fowl of present invention antiviral compound group/lyophilized powder is safe and effective, has no side effect, noresidue harm, can be according to fowl
Virosis selects corresponding yolk antibody, transfer factor and interferon, then coordinates Chinese herbal medicine extract.The present invention is led to
Overtesting further demonstrates that, this freeze-dried powder preparation has good prophylactic-therapeutic effect to avian viral diseases, is far longer than single
The effect used or be used in combination two-by-two.
The method that the present invention prepares fowl antiviral lyophilized powder is simple, it is easy to operation, is suitable to large-scale production and popularization and application.
Use healthy chicken flock as immunization, the probability of strain reversion can be reduced, can quickly improve antibody titer extremely simultaneously
1:256, is highlighted the prevention effect of viral disease by the yolk antibody made after chicken reinforced immunological, and instant effect, curative effect are aobvious
Write, with strong points.
Detailed description of the invention
The present invention is only described in further detail by following embodiment, but does not constitute any limitation of the invention.
Embodiment 1
Fowl antiviral composition in the present embodiment, is made up of the component of following parts by weight: anti-newcastle disease yolk antibody 90
Part, anti-newcastle disease transfer factor 2 parts, alpha-interferon 1 part, astragalus polysaccharides 10 parts, Radix Angelicae Sinensis polysaccharide 5 parts, lycium barbarum polysaccharide
20 parts, Angelica Polysaccharide 10 parts.
Fowl antiviral lyophilized powder in the present embodiment, is made up of the component of following parts by weight: anti-newcastle disease yolk antibody 90
Part, anti-newcastle disease transfer factor 2 parts, alpha-interferon 1 part, astragalus polysaccharides 10 parts, Radix Angelicae Sinensis polysaccharide 5 parts, lycium barbarum polysaccharide
20 parts, Angelica Polysaccharide 10 parts, thimerosal 0.01 part, 0.3 part of formaldehyde, vitamin C 0.5 part, vitamin E2 part, poly-
Vinylpyrrolidone 1 part, glycine 1 part.
The fowl preparation method of antiviral lyophilized powder in the present embodiment, comprises the following steps:
(1) isolated strain prepares vaccine: prepare breast after taking national standard newcastle disease strain Clone30 strain propagation, inactivation
Oil-adjuvant vaccine is standby；
(2) immune health chicken group prepares high-immunity egg: take vaccine virus immunization healthy chicken flock prepared by step (1), every 7 days
To healthy chicken flock immunity once, immunity three times altogether, immunity inoculation inactivated vaccine 1.5mL for the first time, for the second time, exempts from for the third time
Epidemic disease increases by 1 times amount, starts to detect antibody titer after second time immunity, when antibody titer reaches 1:256, collects height and exempts from
Dry standby after Ovum Gallus domesticus album reason, sterilization；
(3) high-immunity egg yolk is separated: take high-immunity egg separation yolk prepared by step (2), add isopyknic sterilized water,
It is stirred well to color turn white to obtain yolk solution, yolk solution is placed in 60 DEG C of water-baths heating 25min, adds pH value
Being 5 DEG C of acidifying waters of 5.0, addition is 6 times of yolk solution volume, staticly settles, take supernatant after mixing under room temperature
Centrifugation 20min under the rotating speed of 15000rpm；
(4) take the supernatant after step (3) is centrifuged, first use the filter membrane of 0.45 μm, with molecular cut off more than 200KD
Macromolecular substances, then use the membrane filtration of 0.22 μm, making the dry weight of anti-newcastle disease yolk antibody in filtrate is 90 parts,
Concentrate, concentrated solution add thimerosal, formaldehyde, vitamin C, vitamin E, polyvinylpyrrolidone, glycine,
After mixing standby through pre-freeze, lyophilizing；
(5) in the dry powder of step (4), anti-newcastle disease transfer factor, alpha-interferon, astragalus polysaccharides, Radix Angelicae Sinensis are added many
Sugar, lycium barbarum polysaccharide and Angelica Polysaccharide powder, mix and get final product.
The preparation method of described anti-newcastle transfer factor is:
(1) clean with the normal saline after sterilizing and use the pig spleen crossed of Avian pneumo-encephalitis virus antigen immune, go its surface muscle,
The tissues such as fat, then use normal saline flushing；
(2) pig spleen after processing crushes, and adds the PBS of 2 times of pre-coolings, evenly homogeneous with tissue homogenate instrument
Serosity；
(3) homogenate ultrasonication process 10min must be crushed liquid；
(4) broken liquid being adjusted to pH value is 5.5, and 5500rpm is centrifuged 30min, collects supernatant；
(5) by supernatant micro-filtrate membrane filtration, the daltonian material of molecular cut off 7000, collect filtrate；
(6) filtrate being used degerming membrane filtration, obtain anti-newcastle transfer factor stock solution, lyophilizing is standby.
Described alpha-interferon is purchased from Changchun Biological Products Institute.
In the present embodiment, fowl is with antiviral lyophilized powder as the application of feed additive, comprises the following steps: with percentage by weight
Meter, adds anti-newcastle disease lyophilized powder in poultry fodder with the amount of 0.4%.
Embodiment 2
Fowl antiviral composition in the present embodiment, is made up of the component of following parts by weight: anti-infectious bronchitis of chicken ovum
Yellow antibody 95 parts, anti-infectious bronchitis of chicken transfer factor 1.5 parts, alpha-interferon 1.5 parts, astragalus polysaccharides 8 parts, when
Return polysaccharide 8 parts, lycium barbarum polysaccharide 15 parts, Angelica Polysaccharide 20 parts.
Fowl antiviral lyophilized powder in the present embodiment, is made up of the component of following parts by weight: anti-infectious bronchitis of chicken ovum
Yellow antibody 95 parts, anti-infectious bronchitis of chicken transfer factor 1.5 parts, alpha-interferon 1.5 parts, astragalus polysaccharides 8 parts, when
Return polysaccharide 8 parts, lycium barbarum polysaccharide 15 parts, Angelica Polysaccharide 20 parts, thimerosal 0.015 part, 0.25 part of formaldehyde, vitamin C 0.8
Part, vitamin e1 .5 part, polyvinylpyrrolidone 1.5 parts, glycine 0.8 part.
In the present embodiment, fowl is malicious with the national standard used in embodiment 1, step (1) by the preparation method of antiviral lyophilized powder
Strain is M41 strain.
In the present embodiment, fowl is with antiviral lyophilized powder as the application of feed additive, comprises the following steps: with percentage by weight
Meter, adds anti-infectious bronchitis of chicken lyophilized powder in poultry fodder with the amount of 0.4%.
Embodiment 3
Fowl antiviral composition in the present embodiment, is made up of the component of following parts by weight: anti-infectious bursal disease ovum
Yellow antibody 100 parts, anti-infectious bursal disease transfer factor 1 part, alpha-interferon 2 parts, astragalus polysaccharides 5 parts, Radix Angelicae Sinensis
Polysaccharide 10 parts, lycium barbarum polysaccharide 10 parts, Angelica Polysaccharide 30 parts.
Fowl antiviral lyophilized powder in the present embodiment, is made up of the component of following parts by weight: anti-infectious bursal disease ovum
Yellow antibody 100 parts, anti-infectious bursal disease transfer factor 1 part, alpha-interferon 2 parts, astragalus polysaccharides 5 parts, Radix Angelicae Sinensis
Polysaccharide 10 parts, lycium barbarum polysaccharide 10 parts, Angelica Polysaccharide 30 parts, thimerosal 0.02 part, 0.2 part of formaldehyde, vitamin C 1
Part, vitamin e1 part, polyvinylpyrrolidone 2 parts, glycine 0.5 part.
In the present embodiment, fowl is malicious with the national standard used in embodiment 1, step (1) by the preparation method of antiviral lyophilized powder
Strain is B87 strain.
In the present embodiment, fowl is with antiviral lyophilized powder as the application of feed additive, comprises the following steps: with percentage by weight
Meter, adds anti-infectious bursal disease lyophilized powder in poultry fodder with the amount of 0.4%.
Embodiment 4
Fowl antiviral composition in the present embodiment, is made up of the component of following parts by weight: anti-infectious laryngotracheitis of chicken ovum
Yellow antibody 96 parts, anti-infectious laryngotracheitis of chicken transfer factor 1.6 parts, alpha-interferon 1.4 parts, astragalus polysaccharides 7 parts, when
Return polysaccharide 7 parts, lycium barbarum polysaccharide 16 parts, Angelica Polysaccharide 22 parts.
Fowl antiviral lyophilized powder in the present embodiment, is made up of the component of following parts by weight: anti-infectious laryngotracheitis of chicken ovum
Yellow antibody 96 parts, anti-infectious laryngotracheitis of chicken transfer factor 1.6 parts, alpha-interferon 1.4 parts, astragalus polysaccharides 7 parts, when
Return polysaccharide 7 parts, lycium barbarum polysaccharide 16 parts, Angelica Polysaccharide 22 parts, thimerosal 0.016 part, 0.22 part of formaldehyde, vitamin C 0.6
Part, vitamin e1 .6 part, polyvinylpyrrolidone 1.4 parts, glycine 0.7 part.
In the present embodiment, fowl is malicious with the national standard used in embodiment 1, step (1) by the preparation method of antiviral lyophilized powder
Strain is SA-2 strain.
In the present embodiment, fowl is with antiviral lyophilized powder as the application of feed additive, comprises the following steps: with percentage by weight
Meter, adds anti-infectious laryngotracheitis of chicken lyophilized powder in poultry fodder with the amount of 0.4%.
Embodiment 5
Fowl antiviral composition in the present embodiment, is made up of the component of following parts by weight: anti-duck plague yolk antibody 92 parts,
Anti-duck plague transfer factor 2 parts, alpha-interferon 2 parts, astragalus polysaccharides 7 parts, Radix Angelicae Sinensis polysaccharide 7 parts, lycium barbarum polysaccharide 16 parts, sweet
Grass polysaccharide 22 parts.
Fowl antiviral lyophilized powder in the present embodiment, is made up of the component of following parts by weight: anti-duck plague yolk antibody 92 parts,
Anti-duck plague transfer factor 2 parts, alpha-interferon 2 parts, astragalus polysaccharides 7 parts, Radix Angelicae Sinensis polysaccharide 7 parts, lycium barbarum polysaccharide 16 parts, sweet
Grass polysaccharide 22 parts, thimerosal 0.016 part, 0.22 part of formaldehyde, vitamin C 0.6 part, vitamin e1 .6 part, polyethylene
Ketopyrrolidine 1.4 parts, glycine 0.7 part.
In the present embodiment fowl by the preparation method of antiviral lyophilized powder with embodiment 1, the national standard duck used in step (1)
Pestilence strain.
Embodiment 6
Fowl antiviral composition in the present embodiment, is made up of the component of following parts by weight: anti-duck viral hepatitis yolk resists
Body 95 parts, anti-duck viral hepatitis transfer factor 1.5 parts, alpha-interferon 1.5 parts, astragalus polysaccharides 8 parts, Radix Angelicae Sinensis polysaccharide 8
Part, lycium barbarum polysaccharide 15 parts, Angelica Polysaccharide 20 parts.
Fowl antiviral lyophilized powder in the present embodiment, is made up of the component of following parts by weight: anti-duck viral hepatitis yolk resists
Body 95 parts, anti-duck viral hepatitis transfer factor 1.5 parts, alpha-interferon 1.5 parts, astragalus polysaccharides 8 parts, Radix Angelicae Sinensis polysaccharide 8
Part, lycium barbarum polysaccharide 15 parts, Angelica Polysaccharide 20 parts, thimerosal 0.015 part, 0.25 part of formaldehyde, vitamin C 0.8 part,
Vitamin e1 .5 part, polyvinylpyrrolidone 1.5 parts, glycine 0.8 part.
In the present embodiment fowl by the preparation method of antiviral lyophilized powder with embodiment 1, the national standard duck used in step (1)
Viral hepatitis strain.
Comparative example 1
In this comparative example, anti-newcastle disease lyophilized powder includes the component of following parts by weight: anti-newcastle disease yolk antibody 90 parts,
Astragalus polysaccharides 10 parts, Radix Angelicae Sinensis polysaccharide 5 parts, lycium barbarum polysaccharide 20 parts, Angelica Polysaccharide 10 parts, other components are with embodiment 1.
In this comparative example, the preparation method of lyophilized powder uses conventional method.
Comparative example 2
In this comparative example, anti-newcastle disease lyophilized powder includes the component of following parts by weight: anti-newcastle disease transfer factor 2 parts,
Astragalus polysaccharides 10 parts, Radix Angelicae Sinensis polysaccharide 5 parts, lycium barbarum polysaccharide 20 parts, Angelica Polysaccharide 10 parts, other components are with embodiment 1.
In this comparative example, the preparation method of lyophilized powder uses conventional method.
Comparative example 3
In this comparative example, anti-newcastle disease lyophilized powder includes the component of following parts by weight: alpha-interferon 1 part, astragalus polysaccharides 10
Part, Radix Angelicae Sinensis polysaccharide 5 parts, lycium barbarum polysaccharide 20 parts, Angelica Polysaccharide 10 parts, other components are with embodiment 1.In this comparative example
The preparation method of lyophilized powder uses conventional method.
Comparative example 4
In this comparative example, anti-newcastle disease lyophilized powder includes the component of following parts by weight: astragalus polysaccharides 10 parts, Radix Angelicae Sinensis polysaccharide 5
Part, lycium barbarum polysaccharide 20 parts, Angelica Polysaccharide 10 parts, other components are with embodiment 1.The preparation side of lyophilized powder in this comparative example
Method uses conventional method.
Comparative example 5
In this comparative example, anti-newcastle disease lyophilized powder includes the component of following parts by weight: anti-newcastle disease yolk antibody 90 parts,
Anti-newcastle disease transfer factor 2 parts, astragalus polysaccharides 10 parts, Radix Angelicae Sinensis polysaccharide 5 parts, lycium barbarum polysaccharide 20 parts, Angelica Polysaccharide 10
Part, other components are with embodiment 1.In this comparative example, the preparation method of lyophilized powder uses conventional method.
Comparative example 6
In this comparative example, anti-newcastle disease lyophilized powder includes the component of following parts by weight: anti-newcastle disease yolk antibody 90 parts,
Alpha-interferon 1 part, astragalus polysaccharides 10 parts, Radix Angelicae Sinensis polysaccharide 5 parts, lycium barbarum polysaccharide 20 parts, Angelica Polysaccharide 10 parts, other groups
Divide with embodiment 1.In this comparative example, the preparation method of lyophilized powder uses conventional method.
Comparative example 7
In this comparative example, anti-newcastle disease lyophilized powder includes the component of following parts by weight: anti-newcastle disease transfer factor 2 parts,
Alpha-interferon 1 part, astragalus polysaccharides 10 parts, Radix Angelicae Sinensis polysaccharide 5 parts, lycium barbarum polysaccharide 20 parts, Angelica Polysaccharide 10 parts, other groups
Divide with embodiment 1.In this comparative example, the preparation method of lyophilized powder uses conventional method.
Comparative example 8
In this comparative example, anti-infectious bronchitis of chicken lyophilized powder includes the component of following parts by weight: resist avian infectious bronchus
Scorching yolk antibody 95 parts, astragalus polysaccharides 8 parts, Radix Angelicae Sinensis polysaccharide 8 parts, lycium barbarum polysaccharide 15 parts, Angelica Polysaccharide 20 parts, its
His component is with embodiment 2.In this comparative example, the preparation method of lyophilized powder uses conventional method.
Comparative example 9
In this comparative example, anti-infectious bronchitis of chicken lyophilized powder includes the component of following parts by weight: resist avian infectious bronchus
Scorching transfer factor 1.5 parts, astragalus polysaccharides 8 parts, Radix Angelicae Sinensis polysaccharide 8 parts, lycium barbarum polysaccharide 15 parts, Angelica Polysaccharide 20 parts, its
His component is with embodiment 2.In this comparative example, the preparation method of lyophilized powder uses conventional method.
Comparative example 10
In this comparative example, anti-infectious bronchitis of chicken lyophilized powder includes the component of following parts by weight: alpha-interferon 1.5 parts,
Astragalus polysaccharides 8 parts, Radix Angelicae Sinensis polysaccharide 8 parts, lycium barbarum polysaccharide 15 parts, Angelica Polysaccharide 20 parts, other components are with embodiment 2.
In this comparative example, the preparation method of lyophilized powder uses conventional method.
Comparative example 11
In this comparative example, anti-infectious bronchitis of chicken lyophilized powder includes the component of following parts by weight: astragalus polysaccharides 8 parts, when
Returning polysaccharide 8 parts, lycium barbarum polysaccharide 15 parts, Angelica Polysaccharide 20 parts, other components are with embodiment 2.Lyophilized powder in this comparative example
Preparation method use conventional method.
Comparative example 12
In this comparative example, anti-infectious bronchitis of chicken lyophilized powder includes the component of following parts by weight: resist avian infectious bronchus
Scorching yolk antibody 95 parts, infectivity resistant bronchitis transfer factor 1.5 parts, astragalus polysaccharides 8 parts, Radix Angelicae Sinensis polysaccharide 8 parts, Chinese holly
Qi polysaccharide 15 parts, Angelica Polysaccharide 20 parts, other components are with embodiment 2.In this comparative example, the preparation method of lyophilized powder uses
Conventional method.
Comparative example 13
Anti-infectious bronchitis of chicken lyophilized powder in this comparative example, including the component of following parts by weight: anti-avian infectious gas
Pipe inflammation yolk antibody 95 parts, alpha-interferon 1.5 parts, astragalus polysaccharides 8 parts, Radix Angelicae Sinensis polysaccharide 8 parts, lycium barbarum polysaccharide 15 parts,
Angelica Polysaccharide 20 parts.In this comparative example, the preparation method of lyophilized powder uses conventional method.
Comparative example 14
In this comparative example, anti-infectious bronchitis of chicken lyophilized powder includes the component of following parts by weight: resist avian infectious bronchus
Scorching transfer factor 1.5 parts, alpha-interferon 1.5 parts, astragalus polysaccharides 8 parts, Radix Angelicae Sinensis polysaccharide 8 parts, lycium barbarum polysaccharide 15 parts, sweet
Grass polysaccharide 20 parts, other components are with embodiment 2.In this comparative example, the preparation method of lyophilized powder uses conventional method.
Comparative example 15
In this comparative example, anti-infections chicken cloacal bursa lyophilized powder includes the component of following parts by weight: anti-infections chicken cloacal bursa ovum
Yellow antibody 100 parts, astragalus polysaccharides 5 parts, Radix Angelicae Sinensis polysaccharide 10 parts, lycium barbarum polysaccharide 10 parts, Angelica Polysaccharide 30 parts, other
Component is with embodiment 3.In this comparative example, the preparation method of lyophilized powder uses conventional method.
Comparative example 16
In this comparative example, anti-infections chicken cloacal bursa lyophilized powder includes the component of following parts by weight: anti-infections chicken cloacal bursa turns
Move the factor 1 part, astragalus polysaccharides 5 parts, Radix Angelicae Sinensis polysaccharide 10 parts, lycium barbarum polysaccharide 10 parts, Angelica Polysaccharide 30 parts, other groups
Divide with embodiment 3.In this comparative example, the preparation method of lyophilized powder uses conventional method.
Comparative example 17
In this comparative example, anti-infections chicken cloacal bursa lyophilized powder includes the component of following parts by weight: alpha-interferon 2 parts, the Radix Astragali
Polysaccharide 5 parts, Radix Angelicae Sinensis polysaccharide 10 parts, lycium barbarum polysaccharide 10 parts, Angelica Polysaccharide 30 parts, other components are with embodiment 3.This is right
In ratio, the preparation method of lyophilized powder uses conventional method.
Comparative example 18
In this comparative example, anti-infections chicken cloacal bursa lyophilized powder includes the component of following parts by weight: astragalus polysaccharides 5 parts, Radix Angelicae Sinensis
Polysaccharide 10 parts, lycium barbarum polysaccharide 10 parts, Angelica Polysaccharide 30 parts, other components are with embodiment 3.Lyophilized powder in this comparative example
Preparation method uses conventional method.
Comparative example 19
In this comparative example, anti-infections chicken cloacal bursa lyophilized powder includes the component of following parts by weight: anti-infections chicken cloacal bursa ovum
Yellow antibody 100 parts, anti-infections chicken cloacal bursa transfer factor 1 part, astragalus polysaccharides 5 parts, Radix Angelicae Sinensis polysaccharide 10 parts, Fructus Lycii
Polysaccharide 10 parts, Angelica Polysaccharide 30 parts, other components are with embodiment 3.In this comparative example, the preparation method of lyophilized powder often uses
Rule method.
Comparative example 20
In this comparative example, anti-infections chicken cloacal bursa lyophilized powder includes the component of following parts by weight: anti-infections chicken cloacal bursa ovum
Yellow antibody 100 parts, alpha-interferon 2 parts, astragalus polysaccharides 5 parts, Radix Angelicae Sinensis polysaccharide 10 parts, lycium barbarum polysaccharide 10 parts, Radix Glycyrrhizae are many
Sugar 30 parts, other components are with embodiment 3.In this comparative example, the preparation method of lyophilized powder uses conventional method.
Comparative example 21
In this comparative example, anti-infections chicken cloacal bursa lyophilized powder includes the component of following parts by weight: anti-infections chicken cloacal bursa turns
Move the factor 1 part, alpha-interferon 2 parts, astragalus polysaccharides 5 parts, Radix Angelicae Sinensis polysaccharide 10 parts, lycium barbarum polysaccharide 10 parts, Angelica Polysaccharide
30 parts, other components are with embodiment 3.In this comparative example, the preparation method of lyophilized powder uses conventional method.
Comparative example 22
In this comparative example, anti-infectious laryngotracheitis of chicken lyophilized powder includes the component of following parts by weight: anti-chicken infectivity throat trachea
Scorching yolk antibody 96 parts, astragalus polysaccharides 7 parts, Radix Angelicae Sinensis polysaccharide 7 parts, lycium barbarum polysaccharide 16 parts, Angelica Polysaccharide 22 parts, its
His component is with embodiment 4.In this comparative example, the preparation method of lyophilized powder uses conventional method.
Comparative example 23
In this comparative example, anti-infectious laryngotracheitis of chicken lyophilized powder includes the component of following parts by weight: anti-chicken infectivity throat trachea
Scorching transfer factor 1.6 parts, astragalus polysaccharides 7 parts, Radix Angelicae Sinensis polysaccharide 7 parts, lycium barbarum polysaccharide 16 parts, Angelica Polysaccharide 22 parts, its
His component is with embodiment 4.In this comparative example, the preparation method of lyophilized powder uses conventional method.
Comparative example 24
In this comparative example, anti-infectious laryngotracheitis of chicken lyophilized powder includes the component of following parts by weight: alpha-interferon 1.4 parts,
Astragalus polysaccharides 7 parts, Radix Angelicae Sinensis polysaccharide 7 parts, lycium barbarum polysaccharide 16 parts, Angelica Polysaccharide 22 parts, other components are with embodiment 4.
In this comparative example, the preparation method of lyophilized powder uses conventional method.
Comparative example 25
In this comparative example, anti-infectious laryngotracheitis of chicken lyophilized powder includes the component of following parts by weight: astragalus polysaccharides 7 parts, when
Returning polysaccharide 7 parts, lycium barbarum polysaccharide 16 parts, Angelica Polysaccharide 22 parts, other components are with embodiment 4.Lyophilized powder in this comparative example
Preparation method use conventional method.
Comparative example 26
In this comparative example, anti-infectious laryngotracheitis of chicken lyophilized powder includes the component of following parts by weight: anti-chicken infectivity throat trachea
Scorching yolk antibody 96 parts, anti-infectious laryngotracheitis of chicken transfer factor 1.6 parts, astragalus polysaccharides 7 parts, Radix Angelicae Sinensis polysaccharide 7 parts,
Lycium barbarum polysaccharide 16 parts, Angelica Polysaccharide 22 parts, other components are with embodiment 4.In this comparative example, the preparation method of lyophilized powder is adopted
Use conventional method.
Comparative example 27
In this comparative example, anti-infectious laryngotracheitis of chicken lyophilized powder includes the component of following parts by weight: anti-chicken infectivity throat trachea
Scorching yolk antibody 96 parts, alpha-interferon 1.4 parts, astragalus polysaccharides 7 parts, Radix Angelicae Sinensis polysaccharide 7 parts, lycium barbarum polysaccharide 16 parts, sweet
Grass polysaccharide 22 parts, other components are with embodiment 4.In this comparative example, the preparation method of lyophilized powder uses conventional method.
Comparative example 28
In this comparative example, anti-infectious laryngotracheitis of chicken lyophilized powder includes the component of following parts by weight: anti-chicken infectivity throat trachea
Scorching transfer factor 1.6 parts, alpha-interferon 1.4 parts, astragalus polysaccharides 7 parts, Radix Angelicae Sinensis polysaccharide 7 parts, lycium barbarum polysaccharide 16 parts, sweet
Grass polysaccharide 22 parts, other components are with embodiment 4.In this comparative example, the preparation method of lyophilized powder uses conventional method.
Test example 1
The embodiment of the present invention 1～6 prepares the quality testing of lyophilized powder.
Test method: take the lyophilized powder of the embodiment of the present invention 1～6 preparation, be placed in 37 DEG C of incubators, preserve 24 months,
Respectively at 1 week, 2 weeks, January, February, April, August, December, 16 months, 20 months, use agar diffusion after 24 months
(AGP) method, measures the titer of yolk antibody in each time point lyophilized powder sample, and result of the test refers to table 1 below.
Table 1 embodiment 1～6 prepares the titer change of yolk antibody in lyophilized powder
The quality testing of yolk antibody:
[mycoplasma inspection] is carried out by " Chinese veterinary pharmacopoeia ", grows without mycoplasma.
[exogenous virus inspection] is carried out by " Chinese veterinary pharmacopoeia ", meets regulation.
[safety verification], with 7 Japanese instar chickling 5, intramuscular injection 5 plumage part, is observed 2 weeks, should all be good for and live, injection site
There is no pathological changes.
[shelf-life], according to room temperature bioactivity experimental result, at least can deposit 2 years.
Test example 2
The effect analysis in terms of preventing and treating Newcastle disease of the lyophilized powder of the embodiment of the present invention 1 preparation.
Test method: choosing 1 monthly age chicken 1000, wherein 100 be only used as counteracting toxic substances group isolated rearing, 100 are only used as blank
Matched group isolated rearing；700 are only used as matched group, are randomly divided into 7 groups, often group 100, isolated rearing, matched group 1～
7 lyophilized powders using comparative example 1～7 preparation successively；Other 100 are only used as test group, and test group uses embodiment 1 to prepare
Lyophilized powder.Feeding manner is free choice feeding, freely drinks water, and takes the chicken intramuscular injection of test group, matched group and counteracting toxic substances group after 24h
Newcastle disease venom after inoculation dilution, continues after infection to feed, test group and matched group every 100ml injection during treatment
The middle lyophilized powder 1g that contains respectively, uses intramuscular injection mode to be administered, 0.1ml/kg body weight, every day 2 times, counteracting toxic substances group intramuscular injection physiology salt
Water, usage and dosage is ibid；Treatment situation after record 120h, the results detailed in table 2 below.
Curative effect judging standard:
Cure: searching for food normal, spirit is normal, without disease；
Effective: to search for food bad, lassitude, but clinical symptom relief；
Invalid: unchanged with before medication.
The prevention effect of table 2 each process group
From table 2 it can be seen that test group reaches 95% to the cure rate of Newcastle disease, the cure rate of matched group be 60～
77.8%,.Statistical analysis shows, the cure rate of test group is significantly higher than matched group (P ＜ 0.01).
Result of the test shows, the lyophilized powder of the embodiment of the present invention 1 preparation can effectively prevent and treat Newcastle disease, and its drug effect is the most excellent
Carry by anti-newcastle disease yolk antibody, anti-newcastle disease transfer factor, alpha-interferon or Chinese medicine in single use or be combined two-by-two
Take lyophilized powder prepared by thing.
Test example 3
The effect analysis in terms of preventing and treating infectious bronchitis of the lyophilized powder of the embodiment of the present invention 2 preparation.
Test method and curative effect judging standard, with test example 2, the difference is that only that test group uses freezing of embodiment 2 preparation
Dry powder, matched group 1～7 uses the lyophilized powder of comparative example 8～14 preparation, uses infectious bronchitis virus counteracting toxic substances.
Result of the test refers to table 3 below.
The prevention effect of table 3 each process group
From table 3 it can be seen that test group reaches 90% to the cure rate of infectious bronchitis, the cure rate of matched group is
66.7～77.8%.Statistical analysis shows, the cure rate of test group is significantly higher than matched group (P ＜ 0.01).
Result of the test shows, the lyophilized powder of the embodiment of the present invention 2 preparation can effectively prevent and treat infectious bronchitis, its medicine
Effect be significantly better than single use or be combined two-by-two by infectivity resistant bronchitis yolk antibody, infectivity resistant bronchitis transfer because of
Lyophilized powder prepared by son, alpha-interferon or Chinese medicine extract.
Test example 4
The effect analysis in terms of preventing and treating infectious bursal disease of the lyophilized powder of the embodiment of the present invention 3 preparation.
Test method and curative effect judging standard, with test example 2, the difference is that only that test group uses freezing of embodiment 3 preparation
Dry powder, matched group 1～7 uses the lyophilized powder of comparative example 15～21 preparation, uses chicken infectivity bursa of Fabricius virus counteracting toxic substances.
Result of the test refers to table 4 below.
The prevention effect of table 4 each process group
From table 4, it can be seen that test group reaches 90% to the cure rate of infectious bursal disease, the cure rate of matched group is
55.6～80%.Statistical analysis shows, the cure rate of test group is significantly higher than matched group (P ＜ 0.01).
Result of the test shows, the lyophilized powder of the embodiment of the present invention 3 preparation can effectively prevent and treat infectious bursal disease, its drug effect
It is significantly better than single use or is combined two-by-two by anti-infectious bursal disease yolk antibody, the transfer of anti-infectious bursal disease
Lyophilized powder prepared by the factor, alpha-interferon or Chinese medicine extract.
Test example 5
The effect analysis in terms of preventing and treating chicken infectious laryngotracheitis of the lyophilized powder of the embodiment of the present invention 4 preparation.
Test method and curative effect judging standard, with test example 2, the difference is that only that test group uses freezing of embodiment 4 preparation
Dry powder, matched group 1～7 uses the lyophilized powder of comparative example 22～28 preparation, uses chicken infectious laryngotracheitis virus counteracting toxic substances.
Result of the test refers to table 5 below.
The prevention effect of table 5 each process group
As can be seen from Table 5, test group reaches 95% to the cure rate of chicken infectious laryngotracheitis, and the cure rate of matched group is
70～80%.Statistical analysis shows, the cure rate of test group is significantly higher than matched group (P ＜ 0.01).
Result of the test shows, the lyophilized powder of the embodiment of the present invention 4 preparation can effectively prevent and treat chicken infectious laryngotracheitis, its medicine
Effect is significantly better than single use or be combined two-by-two and is turned by anti-infectious laryngotracheitis of chicken yolk antibody, anti-infectious laryngotracheitis of chicken
Move lyophilized powder prepared by the factor, alpha-interferon or Chinese medicine extract.
Claims (6)
1. a fowl antiviral composition, it is characterised in that: it is made up of the component of following parts by weight: fowl antiviral yolk antibody 90～100 parts, fowl antiviral transfer factor 1～2 parts, alpha-interferon 1～2 parts, astragalus polysaccharides 5～10 parts, Radix Angelicae Sinensis polysaccharide 5～10 parts, lycium barbarum polysaccharide 10～20 parts, Angelica Polysaccharide 10～30 parts；
Described fowl antiviral yolk antibody and fowl antiviral transfer factor are anti-newcastle disease yolk antibody and anti-newcastle disease transfer factor, anti-infectious bronchitis of chicken yolk antibody and anti-infectious bronchitis of chicken transfer factor, anti-infectious bursal disease yolk antibody and anti-infectious bursal disease transfer factor, anti-infectious laryngotracheitis of chicken yolk antibody and anti-infectious laryngotracheitis of chicken transfer factor, anti-duck plague yolk antibody and anti-duck plague transfer factor or anti-duck virus hepatitis yolk antibody and anti-duck viral hepatitis transfer factor.
2. a fowl antiviral lyophilized powder, it is characterised in that: mainly it is made up of the component of following parts by weight: fowl antiviral yolk antibody 90～100 parts, fowl antiviral transfer factor 1～2 parts, alpha-interferon 1～2 parts, preservative 0.01～0.02 part, inactivator 0.2～0.3 part, heat-resisting lyophilized protecting agent 3～6 parts, astragalus polysaccharides 5～10 parts, Radix Angelicae Sinensis polysaccharide 5～10 parts, lycium barbarum polysaccharide 10～20 parts, Angelica Polysaccharide 10～30 parts；
Described fowl antiviral yolk antibody and fowl antiviral transfer factor are anti-newcastle disease yolk antibody and anti-newcastle disease transfer factor, anti-infectious bronchitis of chicken yolk antibody and anti-infectious bronchitis of chicken transfer factor, anti-infectious bursal disease yolk antibody and anti-infectious bursal disease transfer factor, anti-infectious laryngotracheitis of chicken yolk antibody and anti-infectious laryngotracheitis of chicken transfer factor, anti-duck plague yolk antibody and anti-duck plague transfer factor or anti-duck virus hepatitis yolk antibody and anti-duck viral hepatitis transfer factor.
Fowl the most according to claim 2 antiviral lyophilized powder, it is characterised in that: described heat-resisting lyophilized protecting agent is made up of the component of following parts by weight: vitamin C 0.5～1 part, vitamin E 1～2 parts, polyvinylpyrrolidone 1～2 parts, glycine 0.5～1 part.
4. according to the antiviral lyophilized powder of the fowl described in Claims 2 or 3, it is characterised in that: the preparation method of described fowl antiviral transfer factor is:
(1) clean the pig spleen using avian viruses antigen immune to cross and/or thymus with the normal saline after sterilizing, go its surface muscle, fatty tissue, then use normal saline flushing；
(2) pig spleen and/or thymus after processing crush, and add the PBS of 2 times of pre-coolings, obtain homogenate with tissue homogenate instrument is homogeneous；
(3) homogenate ultrasonication process 5～10min must be crushed liquid；
(4) broken liquid being adjusted to pH value is 5.3～5.7, and 5000～6000rpm are centrifuged 30min, collect supernatant；
(5) by supernatant micro-filtrate membrane filtration, the daltonian material of molecular cut off 7000, collect filtrate；
(6) filtrate being used degerming membrane filtration, obtain fowl antiviral transfer factor stock solution, 4 DEG C of preservations or lyophilizing are standby.
5. the fowl preparation method of antiviral lyophilized powder as according to any one of claim 2 ~ 4, it is characterised in that: comprise the following steps:
(1) isolated strain prepares vaccine: prepare emulsifiable concentrate vaccine after taking Strain propagation, inactivation standby；
(2) immune health chicken group prepares high-immunity egg: take vaccine virus immunization healthy chicken flock prepared by step (1), when antibody titer reaches 1:256, dries standby after collecting high-immunity egg cleaning, sterilization；
(3) high-immunity egg yolk is separated: take high-immunity egg separation yolk prepared by step (2), add isopyknic sterilized water, it is stirred well to color turn white to obtain yolk solution, yolk solution is placed in 60～65 DEG C of water-baths heating 20～30min, add the pre-cooling acidifying water that pH value is 4.9～5.2, addition is 6～8 times of yolk solution volume, staticly settles after mixing under room temperature, takes supernatant centrifugation；
(4) supernatant after step (3) is centrifuged is taken; filter; making the dry weight of fowl antiviral yolk antibody in filtrate is 90～100 parts; concentrate; in terms of parts by weight; adding preservative 0.01～0.02 part, inactivator 0.2～0.3 part, heat-resisting lyophilized protecting agent 3～6 parts in concentrated solution, pre-freeze after mixing, lyophilizing are standby；
(5) in terms of parts by weight, the dry powder of step (4) adds fowl antiviral transfer factor 1～2 parts, alpha-interferon 1～2 parts, astragalus polysaccharides 5～10 parts, Radix Angelicae Sinensis polysaccharide 5～10 parts, lycium barbarum polysaccharide 10～20 parts, Angelica Polysaccharide 10～30 parts, to obtain final product.
6. the fowl as according to any one of claim 2 ~ 4 with antiviral lyophilized powder as the application of feed additive, it is characterized in that: comprise the following steps: by weight percentage, fowl antiviral lyophilized powder is added in poultry fodder with the amount of 0.2～0.6%.
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| CN105664156A (en) * | 2016-03-15 | 2016-06-15 | 济南大东农生物技术有限公司 | Vitamin antibody transfer factor compound oil emulsion for animals and preparing method thereof |
| CN105801683B (en) * | 2016-03-31 | 2019-05-14 | 天津瑞普生物技术股份有限公司 | A kind of dog interferon freeze-dried products |
| CN105749278B (en) * | 2016-05-09 | 2020-09-08 | 无锡市动物疫病预防控制中心 | Composition for resisting porcine pseudorabies, preparation method and application |
| CN106310253A (en) * | 2016-08-29 | 2017-01-11 | 青岛润达生物科技有限公司 | Composite product of ganoderma lucidum polysaccharides and egg yolk antibody |
| CN108096416A (en) * | 2017-12-08 | 2018-06-01 | 重庆金宇恒科技有限公司 | The composition, freeze-dried powder and preparation method of a kind of anti-bronchitis virus of fowl |
| CN108096523A (en) * | 2017-12-08 | 2018-06-01 | 重庆昇顺科技有限公司 | A kind of composition of fowl hepatitis virus resisting, preparation method and application |
| CN110151978A (en) * | 2019-04-19 | 2019-08-23 | 倪同艳 | A kind of fowl multiple cytokine and its production method |
| CN111480746A (en) * | 2020-04-26 | 2020-08-04 | 黑龙江省农业科学院畜牧兽医分院 | Preparation method and application of feed additive for resisting hydropericardium syndrome of chickens |
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| CN101293915A (en) * | 2008-06-17 | 2008-10-29 | 天津生机集团股份有限公司 | Method for preparing anti-duck plague transfer factor |
| CN102716483A (en) * | 2012-05-31 | 2012-10-10 | 郑州后羿制药有限公司 | Duck plague yolk antibody freeze-dried powder and preparation method thereof |
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