CN102302778A - Preparation method and application of traditional Chinese medicinal immunoglobulin compound preparation - Google Patents
Preparation method and application of traditional Chinese medicinal immunoglobulin compound preparation Download PDFInfo
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Abstract
The invention discloses a production method of an immunoglobulin compound preparation for especially preventing and treating porcine diarrhea, and relates to the field of veterinary drugs and feed additives. The production method comprises the following steps: adding part of extracts or powder and the like to specific immunoglobulin or a substance which is not extracted and contains the specific immunoglobulin according to a certain proportion; and then evenly mixing to obtain the immunoglobulin compound preparation. By compositing the advantages of the immunoglobulin extract or powder, a novel antibiotic substitute is formed, thereby achieving the purposes of treating both symptoms and causes, accelerating the rehabilitation speed of a diarrheal animal, preventing and treating porcine diarrhea and meanwhile lowering cost; and the immunoglobulin compound preparation is a safe and hormone-free green product with special good effect on porcine diarrhea. The immunoglobulin compound preparation is taken as a feed additive or mixed with water and drunk. The immunoglobulin compound preparation is especially applicable to prevention and treatment of porcine diarrhea and also suitable for diarrheal diseases of animals such as various poultry, livestock and the like.
Description
Technical field
The present invention relates to veterinary drug and feed additive field, specifically be meant the production method of specially good effect control diarrhea of pigs Chinese herbal medicine immunoglobulin compound formulation.
The present invention is applicable to the prevention and the treatment of diarrhea of pigs, also is applicable to the control of animal diarrhea diseases such as various poultry, domestic animal simultaneously.
Background technology
Piglet diarrhea is a kind of common transmittable disease that is caused by pig Escherichia coli, clostridieum welchii and rotavirus, Transmissible gastroenteritis virus, epidemic diarrhea virus and bacillus perfringens etc.; Comprise transmissible gastroenteritis of swine, epidemic diarrhea, porcine rotavirus disease, porcine reproductive respiratory syndrome, swine fever, pseudorabies and porcine circovirus enteritis etc.; Have characteristics such as morbidity is anxious, propagation is fast, mortality rate height, the development of serious harm pig industry.
Common piglet gastroenteropathy has piglet pujos blancos, yellow scour of piglet, baby swine paratyphoid, transmissible gastroenteritis etc.Piglet is yellow, Hakuri mainly is to be caused by dust Xi Shi escherichia coli, and the piglet sickness rate of generally sucking the breast is higher, and brood piglet sickness rate can reach 80%, and wherein the mortality rate of piglet pujos blancos is lower, and the mortality rate of yellow scour of piglet is very high, sometimes even can full nest dead.(Enterotoxigenic Escherichia colt ETEC) is one of the main pathogenic bacterium (Yokoyam etc., 1992) that cause newborn and diarrhea of weaned piglets to enterotoxigenic escherichia coli.The immunosuppressant that the immature and early weaning of newborn piglet and early-weaned piglets autoimmune function stress cause, be cause piglet to ETEC sensitivity increase, premunition descends, and causes the principal element of ETEC infectious diarrhea.
For a long time, the control of piglet diarrhea mainly depends on antibacterials, escherichia coli multivalence Seedling and recombinant vaccine.Yet antibiotic can cause drug residue in animal body and develop immunity to drugs; Use the vaccine immunity sow, though can improve immunoglobulin content in the breast milk, the maternal immunity globulin persistent period is short, is not enough to protect the piglet whole growth stage not pathogenic; Though oral height exempts from that animal serum can effectively prevent and treat should disease, higher being difficult to of cost applied aborning.
Antibiotic is widely used in the production as the active drug of control of bacterial infection, but excess uses antibiotic phenomenon more and more outstanding in drug resistance problem that antibiotic life-time service causes and the production, but the control effect is also pessimistic.The research of antibiotic succedaneum becomes the urgent needs of animal husbandry development.The development novel formulations, aspect control piglet diarrhea disease, accomplishing to take stopgap measures combines with effecting a permanent cure, and accelerates the development of pig industry, realizes the green innoxious of pork product, becomes the focus of people's concern.
Summary of the invention
The objective of the invention is to; The method for preparing of control diarrhea of pigs Chinese herbal medicine immunoglobulin compound formulation is proposed; On the basis of Chinese herbal medicine base material, adding the special yolk immunoglobulin forms; The present invention combines the advantage of Chinese herbal medicine and immunoglobulin; It is a kind of novel antibiotic succedaneum; Can substitute antibiotic, solve and traditionally produced the drug resistance problem, realize the green innoxious of animal products with the antibiotic therapy diarrhea of pigs.
A kind of method for preparing of Chinese medicine immunoglobulin compounding agent; This Chinese medicine immunoglobulin compounding agent adds the special yolk immunoglobulin and processes on Chinese herbal medicine base material basis; Described Chinese herbal medicine base material is with 2～14 kinds of raw material blending and mixings in Radix Codonopsis, Radix Sophorae Flavescentis, the Radix Pulsatillae, Rhizoma Coptidis, the Radix Astragali, Radix Isatidis, Fructus Forsythiae, Herba Patriniae, Poria, Flos Lonicerae, Herba Portulacae, Herba Plantaginis, Herba Houttuyniae, the Radix Glycyrrhizae; Mix after crushed; Make Chinese herbal medicine powder; Perhaps utilize its effective ingredient of ultrasonic extraction, make the Chinese herbal medicine extracting medicinal powder; Described specific immune globulin is to utilize the unit price or the multivalent inactivated vaccine immunity laying hen of diarrhea of pigs Escherichia coli, clostridieum welchii and rotavirus, Transmissible gastroenteritis virus, epidemic diarrhea virus and bacillus perfringens preparation; Its immune egg again through sterilization, separation purifying technique is prepared from, or by the serum immune globulin of preparations such as above-mentioned vaccine immunity mammal such as pig, rabbit; Solid or liquid preparation that Chinese herbal medicine that process is pulverized or extracted and specific immune globulin or yolk, yolk egg white form according to certain proportioning and the compound mixing of carrier.
The method for preparing of described control diarrhea of pigs Chinese herbal medicine immunoglobulin compound formulation, each Chinese medicine base material weight is identical.
The method for preparing of described control diarrhea of pigs Chinese herbal medicine immunoglobulin compound formulation, compound formulation is powder, water preparation, granule or other dosage forms, can do feed additive and various medicament.
The method for preparing of described control diarrhea of pigs Chinese herbal medicine immunoglobulin compound formulation, Chinese herbal medicine base material adopt and pulverize, broken, the micronizing of micropowder, or the extract after pulverizing, and the ratio that in compound formulation, adds is 1～99%.
The method for preparing of described control diarrhea of pigs Chinese herbal medicine immunoglobulin compound formulation; The special yolk immunoglobulin is for extracting; Or the specific immune egg is directly beaten eggs after sterilizing; Utilize the yellow and white or only use egg yolk; Again with right 3 in Chinese herbal medicine mix, the ratio that in compound formulation, adds is 0.1～99%.
The method for preparing of described control diarrhea of pigs Chinese herbal medicine immunoglobulin compound formulation; Utilize the unit price or the multivalent inactivated vaccine immunity laying hen of preparations such as diarrhea of pigs Escherichia coli, clostridieum welchii and rotavirus, Transmissible gastroenteritis virus, epidemic diarrhea virus and bacillus perfringens, its immune egg adopts separation purifying technique to be prepared from.
The method for preparing of described control diarrhea of pigs Chinese herbal medicine immunoglobulin compound formulation, carrier comprises organic carrier and inorganic carrier.
1. through using pig Escherichia coli K88, K99,9879, F41 vaccine immunity laying hen, the anti-swine escherichia coli specificity of chicken high-immunity yolk immunoglobulin or specific serum immunoglobulin have been prepared.Use flat membrane ultrafiltration technology the immunoglobulin in the yolk is separated, adopt vacuum freeze-drying technique, prepared the Yolk immunoglobulin powder.
2. confirm the Chinese herb drugs prescription composition, utilized ultrasonic technology that Chinese herbal medicine is extracted, adopted vacuum freeze-drying technique, prepared Chinese herbal medicine powder.Utilize Chinese herbal medicine powder diarrhea of mouse model to carry out the therapeutic test; Chinese herbal medicine Yolk immunoglobulin feed additive to different proportionings has carried out extracorporeal bacteria inhibitor test; Confirmed best proportioning; Developed Chinese herbal medicine Yolk immunoglobulin feed additive (piglet diarrhea health) product, early-weaned piglets diarrhoea has been carried out the therapeutic experimental study.
3. the present invention innovates part: the one, developed Chinese herbal medicine Yolk immunoglobulin composite feed additive novel product; The 2nd, set up Chinese herbal medicine and Yolk immunoglobulin extraction process.
4. have innovation aspect the additive formulations two that is combined to form at method for distilling, Yolk immunoglobulin and the Chinese herbal medicine of Yolk immunoglobulin, achievement in research has reached leading domestic level.
5. raw material sources are extensive, and are renewable; Technological process is simple, mild condition; Whole preparation process does not have any waste water, waste gas and waste liquid produces, non-environmental-pollution.
6. the present invention combines the advantage of Chinese herbal medicine and immunoglobulin, is a kind of novel antibiotic succedaneum, has antibiotic, the immunologic function of enhancing body, can suppress the growth of pathogenic entero becterias such as Escherichia coli, is that to keep the healthy institute of piglet essential.Have novelty, can substitute antibiotic, vast market prospect is arranged.
The specific embodiment
Embodiment 1:
Raw material: Chinese herbal medicine extract, Yolk immunoglobulin, carrier
1.1 Chinese herbal medicine extract preparation:
According to Chinese herbal medicine such as the Radix Pulsatillae, Flos Lonicerae, Herba Portulacae, Radix Sophorae Flavescentis, Herba Plantaginis, Herba Portulacae, Herba Houttuyniae, Radix Glycyrrhizae 1: 1: 1: 1: 1: 1: 1: 1 part by weight mixed; Utilizing atomizer to pulverize the back mixes; Utilize its effective ingredient of ultrasonic extraction again, the soak time before extracting is 60～90min.Ultrasonic frequency adopts 20～40kHz, ultrasonic Treatment 30min.After the filtration, process powder through low-temperature freeze-drying machine or spray drying.
1.2 Yolk immunoglobulin preparation:
1.2.1 egg immunity preparation: select healthy bird inlay; With commercial or homemade escherichia coli polyvalent vaccine (like K88, K99,987P, F41 polyvalent vaccine) the laying hen chest muscle is distinguished multi-point injection; 0.5mL/ only; Immunity once weekly; After three immunity; Immunoglobulin level reaches height tire after, collect egg (being high-immunity egg).
1.2.2 Yolk immunoglobulin method for distilling (is example with acetic acid-sodium acetate buffer method)
High-immunity egg with the dirt of clear water flush away eggshell surface, immerses in the 0.5% bromo geramine solution earlier, soaks 10min, the ethanol flushing of reuse 75%, and the aseptic eggshell of breaking is poured in the container that disinfects, and isolates yolk with the sterilization spoon and puts into sterile chamber.1 volume egg yolk adds the acetic acid-sodium acetate buffer (0.05mol/L, pH are 5.0) of 9 volumes, and the two mixing time is 15min, and mixing, leaves standstill 10h by 4 ℃.It is centrifugal that (1900r/min, 10min), abandons deposition by 10 ℃.Collecting precipitation; Deposition is dissolved in 25mmol/L; Make it become the suspension of homogeneous in the NaCI solution; 4 ℃ of following filter paper filterings are removed lipoprotein deposition wherein; Collecting filtrating (contains the water-solubility protein component of Yolk immunoglobulin-WSF), obtains content and the activity of Yolk immunoglobulin crude extract lyophilisation → mensuration Yolk immunoglobulin → process Yolk immunoglobulin preparation
1.3. Chinese medicine immunoglobulin compound formulation preparation
1.3.1 according to Chinese herbal medicine extracting powder, Yolk immunoglobulin powder dry powder and 1: 0.5: 2 part by weight of corn core carrier; Earlier Chinese herbal medicine extracting powder and Yolk immunoglobulin powder are joined mixer in proportion; Mix 1min; Add carrier mixing 4min again, promptly get Chinese medicine immunoglobulin compound formulation.
1.3.2 utilize the ultrasonic extraction Chinese herbal medicine effective ingredients, merging filtrate, being concentrated into original liquid concentration is 1g/mL.The Chinese herbal medicine liquid extract that makes and liquid egg yolk immunoglobulin mixed → process Chinese herbal medicine immunoglobulin liquid → (75～80 ℃ of temperature, back → finished product is handled in the content of time 3～8min) → mensuration Yolk immunoglobulin and activity → bottling to adopt microwave sterilizating then according to 1: 1 part by weight.
Embodiment 2:
Raw material: Chinese herbal medicine extract, yolk, carrier
2.1. Chinese herbal medicine extract preparation: with Chinese herbal medicine with Radix Codonopsis, the Radix Pulsatillae, the Radix Astragali, Radix Isatidis, Herba Patriniae 1: 1: 1: 1: 1 part by weight mixes; After utilizing atomizer to pulverize the back mixing; Water is cooked solvent; After soak time is 60～90min; Utilize ultrasonic instrument again; Carry out ultrasonic Treatment, ultrasonic frequency adopts 20～40kHz, ultrasonic Treatment 30min; After the filtration, process powder through low-temperature freeze-drying machine or spray drying again, obtain the effective ingredient of Chinese herbal medicine extracting compositions.
2.2 yolk preparation:
Get fresh high-immunity egg through 75% alcohol disinfecting after, the manual work method of beating eggs obtains egg yolk liquid or obtains egg yolk liquid through Ovum Gallus domesticus album/egg yolk separator again.
2.3 Chinese herbal medicine and the preparation of yolk compound formulation:
With the Chinese herbal medicine extract in above-mentioned 2.1 and yolk according to the antioxidant BHT → mixer mixing 4min of 1: 1 the defatted rice bran that mixes back → interpolation 20～80% part by weight of weight ratio, behind the mixing → ultraviolet disinfection 1min → utilize vacuum packing machine to carry out vacuum packaging → Chinese herbal medicine immunoglobulin powder product with 0.5% part by weight.
Embodiment 3:
Raw material: Chinese herbal medicine extract, yolk, egg white, carrier
3.1 Chinese herbal medicine extract preparation: with Chinese herbal medicine with Radix Sophorae Flavescentis, the Radix Pulsatillae, Rhizoma Coptidis, the Radix Astragali, Radix Isatidis, Herba Patriniae, Radix Glycyrrhizae 1: 1: 1: 1: 1: 1: 1 part by weight mixed; Utilizing atomizer to pulverize the back mixes; Utilize its effective ingredient of ultrasonic extraction again, the soak time before extracting is 60～90min.Ultrasonic frequency adopts 20～40kHz, ultrasonic Treatment 30min.After the filtration, process powder through low-temperature freeze-drying machine or spray drying.
3.2 yolk, egg white preparation:
After getting fresh high-immunity egg sterilization, beat eggs and get yolk Ovum Gallus domesticus album, remove shell breaking.
3.3 Chinese herbal medicine yolk egg white formulation preparation:
The Chinese herbal medicine extract that makes and yolk and egg white are carried out the white carbon black of proportioning → interpolation 20～60% part by weight according to 1: 1 part by weight, through the content and the activity → vacuum packaging → finished product of mixer mixing → sterilization → Chinese herbal medicine immunoglobulin powder → mensuration Yolk immunoglobulin
Embodiment 4:
Raw material: the broken or micronizing of Chinese herbal medicine micropowder, Yolk immunoglobulin, carrier
4.1 the broken or micronizing of Chinese herbal medicine micropowder:
According to Chinese herbal medicine such as Radix Sophorae Flavescentis, the Radix Pulsatillae, Rhizoma Coptidis, the Radix Astragali, Radix Isatidis, Herba Patriniae, Herba Houttuyniae, Radix Glycyrrhizae 1: 1: 1: 1: 1: 1: 1: 1 part by weight mixed, and utilized atomizer to pulverize the back and mixed, the preparation Chinese herbal medicine powder.
4.2 Yolk immunoglobulin preparation (employing water dilution method):
High-immunity egg with the dirt of clear water flush away eggshell surface, immerses in the 0.5% bromo geramine solution earlier, soaks 10min, the ethanol flushing of reuse 75%, the aseptic eggshell of breaking.Separate Ovum Gallus domesticus album and yolk; Yolk adds 9 times of sterilized water dilutions; After the mixing of HCI adjusting pH5.2 diluent; In 4 ℃ of cold preservation 5h; Centrifugal 1h under 4 ℃ of 1000g conditions, supernatant add saturated ammonium sulfate and the sodium sulfate deposition of saltouing, centrifugal after; Through low-temperature freeze-drying machine or spray drying, promptly get and slightly carry Yolk immunoglobulin.
4.3 Chinese medicine immunoglobulin compound formulation preparation:
According to Chinese herbal medicine extracting powder, Yolk immunoglobulin powder dry powder and 1: 0.5: 2 part by weight of corn core carrier; Earlier Chinese herbal medicine extracting powder and Yolk immunoglobulin powder are joined mixer in proportion; Mix 1min; Add 20～60% white carbon blacks, through mixer mixing 4min → sterilization → Chinese herbal medicine immunoglobulin powder → vacuum packaging → finished product.
Embodiment 5:
Raw material: the broken or micronizing of Chinese herbal medicine micropowder, yolk, carrier
5.1 Chinese herbal medicine extract preparation:
According to Chinese herbal medicine such as the Radix Pulsatillae, Flos Lonicerae, the Radix Pulsatillae, Radix Sophorae Flavescentis, Herba Plantaginis, the Radix Astragali, Herba Houttuyniae, Radix Glycyrrhizae 1: 1: 1: 1: 1: 1: 1: 1 part by weight mixed; Utilizing atomizer to pulverize the back mixes; Utilize its effective ingredient of ultrasonic extraction again, the soak time before extracting is 60～90min.Ultrasonic frequency adopts 20～40kHz, ultrasonic Treatment 30min.After the filtration, process powder through low-temperature freeze-drying machine or spray drying.
5.2 yolk preparation:
After getting fresh high-immunity egg sterilization, manual work is beaten eggs and is got yolk or obtain egg yolk liquid through Ovum Gallus domesticus album/egg yolk separator.
5.3 Chinese herbal medicine and the preparation of yolk compound formulation:
Mix back → interpolation 20～80% defatted rice brans and 0.5% antioxidant → according to 1: 1 part by weight of Chinese herbal medicine extract and yolk weight ratio through mixer mixing 4min → sterilization → vacuum packaging → Chinese herbal medicine immunoglobulin powder product.
Embodiment 6:
Raw material: the broken or micronizing of Chinese herbal medicine micropowder, yolk egg white, carrier
6.1 according to Chinese herbal medicine such as Radix Sophorae Flavescentis, the Radix Pulsatillae, Rhizoma Coptidis, Herba Plantaginis 1: 1: 1: 1: 1: 1 part by weight mixes, and utilizes micronizing or atomizer to pulverize the back and mixes, and makes Chinese herbal medicine powder.
6.2 yolk egg white preparation:
After getting fresh high-immunity egg sterilization, beat eggs and get yolk Ovum Gallus domesticus album, remove shell breaking or obtain egg yolk liquid through Ovum Gallus domesticus album/egg yolk separator.
6.3 Chinese herbal medicine yolk egg white formulation preparation:
The Chinese herbal medicine powder that makes and yolk Ovum Gallus domesticus album are carried out the white carbon black of proportioning → interpolation 20～60% part by weight according to 1: 1 part by weight, through the content and the activity → vacuum packaging → finished product of mixer mixing → sterilization → Chinese herbal medicine immunoglobulin powder → mensuration Yolk immunoglobulin
Need to prove; Chinese herbal medicine of the present invention can also be selected from 2～14 kinds of raw materials in Radix Codonopsis, Radix Sophorae Flavescentis, the Radix Pulsatillae, Rhizoma Coptidis, the Radix Astragali, Radix Isatidis, Fructus Forsythiae, Herba Patriniae, Poria, Flos Lonicerae, Herba Portulacae, Herba Plantaginis, Herba Houttuyniae, the Radix Glycyrrhizae, forms according to the principle blending and mixing of weight such as various raw materials.
Embodiment 7
The application of Chinese medicine immunoglobulin compounding agent
Adopt spice, drinking-water or irritate clothes.
Yellow scour of piglet clinical practice dosage: 3.5g/d. head; Piglet pujos blancos clinical practice dosage: 7.0g/d. head.The Chinese medicine immunoglobulin compounding agent adopts the method for embodiment 1 to make.
(1) Chinese medicine immunoglobulin compounding agent treatment yellow scour of piglet clinical trial
80 of sick pigs that natural infection are diagnosed as yellow scour of piglet are divided into 4 groups at random, 20 every group.The high, medium and low dose groups of medicine every filling is respectively obeyed 5g, 3.5g, 2g Chinese medicine immunoglobulin compounding agent, and the medicine matched group is irritated the clothes 3.5g Radix Pulsatillae and loose, every day 2 times, continuous 3d.Observe disease pig record spirit, appetite and dysentery situation every day after the administration, observes the 7th day statistics curative effect to the drug withdrawal; Respectively at before the administration with drug withdrawal after the 2nd day blood sample collection measure the variation of hemoglobin (HB), erythrocyte (RBC) sum, leukocyte (WBC) sum.
The result shows: the Chinese medicine immunoglobulin compounding agent is high, the cure rate of middle dose groups is respectively 95%, 85%, is significantly higher than medicine matched group (70%) and medicine low dose group.Medicine is high, the sick pig number of middle dose groups reduces rapidly, and symptom alleviates rapidly, and the cure rate and the total effective rate of high dose group are the highest, is middle dose groups secondly, and two groups are significantly higher than low dose group and medicine matched group, and the difference between two groups is not remarkable; Organize respectively after the treatment that Hb content and RBC sum raise to some extent, WBC decreases, but there was no significant difference (P＞0.05).Show that the Chinese medicine immunoglobulin compounding agent is high, middle dose groups has curative effect preferably to yellow scour of piglet, dosage in the recommendation (3.5g/ head) is clinical practice dosage.
(2) Chinese medicine immunoglobulin compounding agent treatment piglet pujos blancos clinical efficacy test
80 of sick pigs (10～20 age in days) that natural infection are diagnosed as piglet pujos blancos are divided into 4 groups, 20 every group at random.The high, medium and low dose groups of medicine every filling is respectively obeyed 10g, 7g, 4g Chinese medicine immunoglobulin compounding agent, and every of medicine matched group is irritated the clothes 12g Radix Pulsatillae and loose, every day 2 times, continuous 3d.Observe disease pig record spirit, appetite and dysentery situation every day after the administration, observes the 7th day statistics curative effect to the drug withdrawal; Respectively at before the administration with drug withdrawal after the 2nd day blood sample collection measure the variation of hemoglobin, erythrocyte (RBC) sum, leukocyte (WBC) sum.Observe " Chinese medicine immunoglobulin compounding agent " clinical treatment result to ill piglet.The result shows: the Chinese medicine immunoglobulin compounding agent is high, the cure rate of middle dose groups is respectively 95%, 90%, is significantly higher than medicine matched group (80%) and medicine low dose group.The Hakuri piglet is after " Chinese medicine immunoglobulin compounding agent " and the diffusing treatment of the Radix Pulsatillae, and the pathogenic infection percentage rate reduces, before and after the treatment piglet pujos blancos, and erythrocyte number, hemoglobin content rising, total white blood cells descends.The best results of high dose group and middle dose groups, but the two no significant difference.The result shows that the Chinese medicine immunoglobulin compounding agent is high, middle dose groups has curative effect preferably to piglet pujos blancos.Dosage in the recommendation (7g/d. head) is clinical practice dosage.
Should be understood that, concerning those of ordinary skills, can improve according to the above description or conversion, and all these improvement and conversion all should belong to the protection domain of accompanying claims of the present invention.
Claims (8)
1. the method for preparing of a Chinese medicine immunoglobulin compounding agent; It is characterized in that; This Chinese medicine immunoglobulin compounding agent adds the special yolk immunoglobulin and processes on Chinese herbal medicine base material basis; Described Chinese herbal medicine base material is with 2～14 kinds of raw material blending and mixings in Radix Codonopsis, Radix Sophorae Flavescentis, the Radix Pulsatillae, Rhizoma Coptidis, the Radix Astragali, Radix Isatidis, Fructus Forsythiae, Herba Patriniae, Poria, Flos Lonicerae, Herba Portulacae, Herba Plantaginis, Herba Houttuyniae, the Radix Glycyrrhizae; Mix after crushed; Make Chinese herbal medicine powder; Perhaps utilize its effective ingredient of ultrasonic extraction, make the Chinese herbal medicine extracting medicinal powder; Described specific immune globulin is to utilize the unit price or the multivalent inactivated vaccine immunity laying hen of diarrhea of pigs Escherichia coli, clostridieum welchii and rotavirus, Transmissible gastroenteritis virus, epidemic diarrhea virus and bacillus perfringens preparation; Its immune egg again through sterilization, separation purifying technique is prepared from, or by the serum immune globulin of preparations such as above-mentioned vaccine immunity mammal such as pig, rabbit; Solid or liquid preparation that Chinese herbal medicine that process is pulverized or extracted and specific immune globulin or yolk, yolk egg white form according to certain proportioning and the compound mixing of carrier.
2. the method for preparing of Chinese medicine immunoglobulin compounding agent according to claim 1 is characterized in that, it is characterized in that, each Chinese medicine base material weight is identical.
3. the method for preparing of Chinese medicine immunoglobulin compounding agent according to claim 1 is characterized in that, it is characterized in that, compound formulation is powder, water preparation, granule or other dosage forms, can do feed additive and various medicament.
4. the method for preparing of Chinese medicine immunoglobulin compounding agent according to claim 1 is characterized in that; It is characterized in that; The Chinese herbal medicine base material adopts and pulverizes, broken, the micronizing of micropowder, or the extract after pulverizing, and the ratio that in compound formulation, adds is 1～99%.
5. the method for preparing of Chinese medicine immunoglobulin compounding agent according to claim 1; It is characterized in that; It is characterized in that; The special yolk immunoglobulin is for extracting; Or the specific immune egg is directly beaten eggs after sterilizing; Utilize the yellow and white or only use egg yolk, mix with said Chinese herbal medicine, the ratio that in compound formulation, adds is 0.1～99%.
6. the method for preparing of Chinese medicine immunoglobulin compounding agent according to claim 1; It is characterized in that; It is characterized in that; Utilize the unit price or the multivalent inactivated vaccine immunity laying hen of preparations such as diarrhea of pigs Escherichia coli, clostridieum welchii and rotavirus, Transmissible gastroenteritis virus, epidemic diarrhea virus and bacillus perfringens, its immune egg adopts separation purifying technique to be prepared from.
7. the method for preparing of Chinese medicine immunoglobulin compounding agent according to claim 1 is characterized in that, it is characterized in that, carrier comprises organic carrier and inorganic carrier.
8. the Chinese medicine immunoglobulin compounding agent is as the application of control diarrhea of pigs medicine.
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