CN101357229A - Production technique of immunoglobulin compounding agent capable of preventing and treating pig diarrhea - Google Patents
Production technique of immunoglobulin compounding agent capable of preventing and treating pig diarrhea Download PDFInfo
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Abstract
The invention relates to the field of veterinary drug and feed additives, in particular to the manufacturing method of specific immunoglobulin combination preparation which cures pig diarrhea. The invention is characterized in that the combination preparation is formed by well blending specificity immunoglobulin or uncultivated extractive containing specificity immunoglobulin which is added with some extractive or power according to a certain ratio. The invention combines the advantages of the immunoglobulin extractive or the power to form a novel antibiotic alternative, achieves treatment focusing on relieving both the secondary and primary symptoms, can speed up the rehabilitation of diarrhea animals, achieves the purpose of preventing disease and treating an illness, simultaneously reduces the cost. The combination preparation is a safe, non-hormone green product, has especially good effect on pig diarrhea with the cure rate more than 98%, and the pig growth rate is increased by more than 10%. The combination preparation is especially suitable for the prevention and treatment of pig diarrhea, and is also suitable for the prevention of a variety of animal diarrhea diseases such as poultry, livestock, etc.
Description
(1) technical field
The present invention relates to veterinary drug and feed additive field, specifically be meant the production method of specially good effect control diarrhea of pigs Chinese herbal medicine immunoglobulin compound formulation.
The present invention is applicable to the prevention and the treatment of diarrhea of pigs, also is applicable to the control of animal diarrhea diseases such as various poultry, domestic animal simultaneously.
(2) background technology
Piglet diarrhea is a kind of common transmittable disease that is caused by pig Escherichia coli, clostridieum welchii and rotavirus, Transmissible gastroenteritis virus, epidemic diarrhea virus and bacillus perfringens etc., comprise transmissible gastroenteritis of swine, epidemic diarrhea, porcine rotavirus disease, porcine reproductive respiratory syndrome, swine fever, pseudorabies and porcine circovirus enteritis etc., have characteristics such as morbidity is anxious, propagation is fast, mortality rate height, the development of serious harm pig industry.
Common piglet gastroenteropathy has piglet pujos blancos, yellow scour of piglet, baby swine paratyphoid, transmissible gastroenteritis etc.Piglet Huang, Hakuri mainly are to be caused by dust Xi Shi escherichia coli, and the piglet sickness rate of generally sucking the breast is higher, and brood piglet sickness rate can reach 80%, and wherein the mortality rate of piglet pujos blancos is lower, and the mortality rate of yellow scour of piglet is very high, sometimes even can full nest death.(Enterotoxigenic Escherichia colt ETEC) is one of the main pathogenic bacterium (Yokoyam etc., 1992) that cause newborn and diarrhea of weaned piglets to enterotoxigenic escherichia coli.The immunosuppressant that the immature and early weaning of newborn piglet and early-weaned piglets autoimmune function stress cause, be cause piglet to ETEC sensitivity increase, premunition descends, and causes the principal element of ETEC infectious diarrhea.
For a long time, the control of piglet diarrhea mainly depends on antibacterials, escherichia coli multivalence Seedling and recombinant vaccine.Yet antibiotic can cause drug residue in animal body and develop immunity to drugs; Use the vaccine immunity sow, though can improve immunoglobulin content in the breast milk, the maternal immunity globulin persistent period is short, is not enough to protect the piglet whole growth stage not pathogenic; Can effectively prevent and treat this disease though oral height is exempted from animal serum, higher being difficult to of cost applied aborning.
Chinese herbal medicine is a pure natural preparation, and toxic and side effects is little, does not have drug resistance and drug residue problem, and in addition along with the improving constantly of people's living standard, the safety of animal food more and more causes the concern of society.Chinese herbal medicine additive can also stimulate the piglet non-specific immunity when improving the piglet specific immune function.Studies show that Chinese herbal medicine additive can significantly improve protease activities in the ablactational baby pig intestinal, thereby a side draws the protein adsorption rate that improves, promote the growth promoter of histoorgan and immune organ; On the other hand, promote proteinic digesting and assimilating, the big intestinal segment microorganism of unlikely quilt excessive fermentation has prevented the diarrheal generation.
Yolk immunoglobulin (Egg Yolk Immunoglobulin, Yolk immunoglobulin), Yolk immunoglobulin has antibiotic, the immunologic function of enhancing body, adopts biotechnology to prepare the specific polyclonal immunoglobulin from laying hen yolk, it is low to have cost, the characteristics that output is high.Yolk immunoglobulin can condense, engulf and in and antibacterial and toxin composition; The enhance immunity systemic-function, diseases such as prevention digestive tract promote alimentation.Be used for the health care of animal and disease treatment etc.The prevention of coliform diarrhea in piglets Yolk immunoglobulin has intensive affinity to the host specificity fimbrial antigen, can block or reduce combining of host specific sex fimbria and piglet small intestine epithelium, make ETEC not settle down breeding at small intestinal, thereby blocked escherichia coli and produced enterotoxin, prevented and reduced the generation of piglet diarrhea.
Antibiotic is widely used in the production as the active drug of control of bacterial infection, but excess uses antibiotic phenomenon more and more outstanding in drug resistance problem that antibiotic life-time service causes and the production, but the control effect is also pessimistic.The research of antibiotic succedaneum becomes the urgent needs of animal husbandry development.The development novel formulations, aspect control piglet diarrhea disease, accomplishing to take stopgap measures combines with effecting a permanent cure, and accelerates the development of pig industry, realizes the green innoxious of pork product, becomes the focus of people's concern.
(3) summary of the invention
The objective of the invention is to, the preparation method of control diarrhea of pigs Chinese herbal medicine immunoglobulin compound formulation is proposed, adding the special yolk immunoglobulin on Chinese herbal medicine base material basis forms, the present invention combines the advantage of Chinese herbal medicine and immunoglobulin, it is a kind of novel antibiotic succedaneum, can substitute antibiotic, solve traditional antibiotic therapy diarrhea of pigs of using and produced the drug resistance problem, realize the green innoxious of animal products.
Technology of the present invention is as follows:
1. the preparation method of Yolk immunoglobulin
1.1 egg immunity preparation: select healthy bird inlay, with commercial or homemade escherichia coli polyvalent vaccine (as K88, K99,987P, F41 polyvalent vaccine) the laying hen chest muscle is distinguished multi-point injection, 0.5mL/ only, immunity once weekly, after three immunity, immunoglobulin level reaches height tire after, collect egg.
1.2 Yolk immunoglobulin extracting method---acetic acid-sodium acetate buffer method ()
High-immunity egg with the dirt of clear water flush away eggshell surface, immerses in the 0.5% bromo geramine solution earlier, soaked 10 minutes, and the ethanol flushing of reuse 75%, the aseptic eggshell of breaking is poured in the container that disinfects, and isolates yolk with the sterilization spoon and puts into sterile chamber.1 volume egg yolk adds the acetic acid-sodium acetate buffer (0.05mol/L, pH are 5.0) of 9 volumes, and the two mixing time is 15min, and mixing, leaves standstill 10h by 4 ℃.It is centrifugal that (1900r/min, 10min), abandons precipitation by 10 ℃.Collecting precipitation, precipitation is dissolved in 25mmol/L, make it become the suspension of homogeneous in the NaCI solution, 4 ℃ of following filter paper filterings are removed lipoprotein precipitation wherein, collect filtrate (the water-solubility protein component that contains Yolk immunoglobulin), obtain the content of Yolk immunoglobulin crude extract lyophilisation → mensuration Yolk immunoglobulin and activity → make Yolk immunoglobulin preparation → powder or injection
1.3 Yolk immunoglobulin extracting method---water dilution method (two)
High-immunity egg with the dirt of clear water flush away eggshell surface, immerses in the 0.5% bromo geramine solution earlier, soaks the ethanol flushing of reuse 75%, the aseptic eggshell of breaking 10 minutes.Separate Ovum Gallus domesticus album and yolk, yolk adds 9 times of sterilized water dilutions, after mixing with HCI adjusting pH5.2 diluent, in 4 ℃ of cold preservation 5h, centrifugal 1h under 4 ℃ of 1000g conditions, supernatant add saturated ammonium sulfate and the sodium sulfate sedimentation method of saltouing and are further purified, the PBS dialysis is and slightly carries Yolk immunoglobulin.Slightly carry Yolk immunoglobulin through DEAE-glucosan A50, after routine is handled,, carry out eluting with buffer behind the last sample with 0.015mol/Ltris～HCI PH8.0 buffer balance 2 * 40cm glass column of packing into.Or through the content and the activity of oversalting, ultrafiltration and concentration, lyophilisation, mensuration Yolk immunoglobulin, make enteric Yolk immunoglobulin capsule or other dosage forms.The also available Polyethylene Glycol of the method for purification (PEG) method, dextran sulfate method etc.
1.4 Yolk immunoglobulin extracting method---ultrafiltration (three)
That utilizes that method (two) obtains slightly carries Yolk immunoglobulin liquid, adopts ultrafiltration, and the molecular retention amount adopts 100kDa, operating pressure difference is that 0.12MPa, feeding temperature are 30 ℃, pH5.2, the trapped fluid immunoglobulin response rate reaches 91.89%, and purity is 86%; At last, obtain highly purified Yolk immunoglobulin through the DEAE ion-exchange chromatography.
1.5 Yolk immunoglobulin extraction process (is example with the egg)
Fresh hen egg → beat eggs is got the content of egg yolk → preparation yolk solution → saltout → ultrafiltration and concentration → lyophilisation → mensuration Yolk immunoglobulin and activity → make Yolk immunoglobulin preparation → powder or injection
2. the preparation method of serum immune globulin
2.1 select healthy animal (as pig, horse, cattle, sheep etc.), adopt oral immunity, injection or collunarium method with commercial or homemade escherichia coli polyvalent vaccine (as K88, K99,987P, F41 polyvalent vaccine), it is carried out immunity, dosage: every 0.1～3.0mL.Take a blood sample after three days in immunity, in 2000～10000r/min, centrifugal 20～30min gets serum after the natural coagulation.
2.2 from blood, extract immunoglobulin.Commonly used have salting out method (as sodium polyphosphate flocculence, ammonium sulfate salting-out process), organic solvent precipitation method (as cold ethanol partition method), the organic polymer sedimentation method, degeneration sedimentation method etc.The method that is used for large-scale production mainly contains: cold ethanol partition method, salting out method, rivanol method and column chromatography etc., using more is ammonium sulfate salting-out process and cold ethanol partition method.
2.3 extraction process (is example with the ammonium sulfate salting-out process):
Porcine blood serum → adding ammonium sulfate saturated solution precipitates → immune globulin (Ig) crude product → pure product of adding distil water transfer → ultrafiltration → dialysis → Ig.Process conditions adopt the (NH of pH7.0～7.2,40～50 ℃, 50% saturation
4)
2SO
4Solution, this technology can large-scale production Ig, safety non-toxic, easy to operate.The biological activity that can guarantee Ig is not damaged, and can satisfy mass-produced needs again.The raw material that is rich in Ig in China is very abundant, with regard to the nationwide, can get Sanguis sus domestica 1125～11,500,000,000 kg every year, if these raw materials can be used well, economic benefit of being created and social benefit are with considerable thus.
2.4 spray drying process
It is 10%～20% solution that the animal blood IgG that extracts is mixed with protein concentration, respectively 120～180 ℃ of air intake vent temperature, carries out spray drying under 60～80 ℃ of conditions of air outlet temperature, and vacuum packaging places refrigerator to preserve.
3. Chinese herbal medicine extract preparation method
According to Chinese herbal medicine such as the Radix Pulsatillae, Flos Lonicerae, Herba Portulacae, Radix Sophorae Flavescentis, Herba Plantaginis, Herba Portulacae, Herba Houttuyniae, Radix Glycyrrhizae 1: 1: 1: 1: 1: 1: 1: 1 mixed, utilize the broken back of micropowder flour to mix, utilize its effective ingredient of ultrasonic extraction again, the soak time before extracting is 60～90min.Ultrasonic frequency adopts 20～40kHz, ultrasonic Treatment 30min.After the filtration, make powder through low-temperature freeze-drying machine or spray drying.
3.1 get crushed a certain amount of for the examination traditional Chinese medicinal material samples for the preparation of test agent extract, place tool plug triangular flask, the extraction solvent that adds times volume, place ultrasonic unit (SB3200), after handling 30min, filter, after filtrate decompression concentrates, handle 15min at the extraction solvent supersonic ripple that adds 5 times of volumes, filter, filtrate decompression concentrates, so repeat 2 times, the concentrate that merges 3 extracts promptly obtains the crude extract of the ultrasonic extraction of this solvent, and it is standby to place 4 ℃ of refrigerators to preserve.
3.2 Chinese herbal medicine extract microcapsule production technology
The ratio of and maltodextrin white 1: 2 according to Semen sojae atricolor, the Chinese herbal medicine extract addition is 2.2%, homogenization pressure is 30MPa, 100 ℃ of spray drying tower air inlet pathogenic wind-warm, 70～80 ℃ of air-out pathogenic wind-warm.The spray-dried Chinese herbal medicine extract microcapsule that makes.
4. Chinese herbal medicine is pulverized
Adopt atomizer or super micron mill that Chinese herbal medicine is pulverized.
5. Chinese herbal medicine and Yolk immunoglobulin feed addictive preparation method
5.1 Chinese herbal medicine extracting powder and Yolk immunoglobulin powder
According to Chinese herbal medicine extracting powder, Yolk immunoglobulin powder dry powder and 1: 0.5: 2 ratio of carrier, earlier Chinese herbal medicine extracting powder and Yolk immunoglobulin powder are joined mixer mixing 1min, add carrier mixing 4min again, promptly get composite feed additive.
5.2 utilize the ultrasonic extraction Chinese herbal medicine effective ingredients, merging filtrate, being concentrated into original liquid concentration is 1g/mL, adopts the batch process sterilization.Make powder through low-temperature freeze-drying machine; The Yolk immunoglobulin powder is prepared through the laying hen immunity by vaccine.Compound Chinese herbal medicine and the Yolk immunoglobulin composite feed additive of having prepared of Chinese herbal medicine extracting powder and Yolk immunoglobulin powder.
Advantage of the present invention is:
1. by using pig Escherichia coli K88, K99,9879, F41 vaccine immunity laying hen, the anti-swine escherichia coli specificity of chicken high-immunity yolk immunoglobulin or specific serum immunoglobulin have been prepared.Use flat membrane ultrafiltration technology the immunoglobulin in the yolk is separated, adopt vacuum freeze-drying technique, prepared the Yolk immunoglobulin powder.
2. determine the Chinese herb drugs prescription composition, utilized ultrasonic technology that Chinese herbal medicine is extracted, adopted vacuum freeze-drying technique, prepared Chinese herbal medicine powder.Utilize Chinese herbal medicine powder diarrhea of mouse model to carry out the therapeutic test; Chinese herbal medicine Yolk immunoglobulin feed additive to different proportionings has carried out extracorporeal bacteria inhibitor test, determined best proportioning, developed Chinese herbal medicine Yolk immunoglobulin feed additive (piglet diarrhea health) product, early-weaned piglets diarrhoea has been carried out the therapeutic experimental study.
3. part is innovated in this research: the one, developed Chinese herbal medicine Yolk immunoglobulin composite feed additive novel product; The 2nd, set up Chinese herbal medicine and Yolk immunoglobulin extraction process.
4. have innovation aspect the additive formulations two that is combined to form at extracting method, Yolk immunoglobulin and the Chinese herbal medicine of Yolk immunoglobulin, achievement in research has reached leading domestic level.
5. raw material sources are extensive, and are renewable; Technological process is simple, mild condition; Whole preparation process does not have any waste water, waste gas and waste liquid produces, non-environmental-pollution.
6. the present invention combines the advantage of Chinese herbal medicine and immunoglobulin, is a kind of novel antibiotic succedaneum, has antibiotic, the immunologic function of enhancing body, can suppress the growth of pathogenic entero becterias such as Escherichia coli, is that to keep the healthy institute of piglet essential.Have novelty, can substitute antibiotic, vast market prospect is arranged.
(4) specific embodiment
The specific embodiment one:
Raw material: Chinese herbal medicine extract, Yolk immunoglobulin, carrier
1. Chinese herbal medicine extract preparation:
According to Chinese herbal medicine such as the Radix Pulsatillae, Flos Lonicerae, Herba Portulacae, Radix Sophorae Flavescentis, Herba Plantaginis, Herba Portulacae, Herba Houttuyniae, Radix Glycyrrhizae 1: 1: 1: 1: 1: 1: 1: 1 mixed, utilize the broken back of micropowder flour to mix, utilize its effective ingredient of ultrasonic extraction again, the soak time before extracting is 60～90min.Ultrasonic frequency adopts 20～40kHz, ultrasonic Treatment 30min.After the filtration, make powder through low-temperature freeze-drying machine or spray drying.
2. Yolk immunoglobulin preparation:
2.1 egg immunity preparation: select healthy bird inlay, with commercial or homemade escherichia coli polyvalent vaccine (as K88, K99,987P, F41 polyvalent vaccine) the laying hen chest muscle is distinguished multi-point injection, 0.5mL/ only, immunity once weekly, after three immunity, immunoglobulin level reaches height tire after, collect egg.
2.2 Yolk immunoglobulin extracting method (is example with acetic acid-sodium acetate buffer method)
High-immunity egg with the dirt of clear water flush away eggshell surface, immerses in the 0.5% bromo geramine solution earlier, soaked 10 minutes, and the ethanol flushing of reuse 75%, the aseptic eggshell of breaking is poured in the container that disinfects, and isolates yolk with the sterilization spoon and puts into sterile chamber.1 volume egg yolk adds the acetic acid-sodium acetate buffer (0.05mol/L, pH are 5.0) of 9 volumes, and the two mixing time is 15min, and mixing, leaves standstill 10h by 4 ℃.It is centrifugal that (1900r/min, 10min), abandons precipitation by 10 ℃.Collecting precipitation, precipitation is dissolved in 25mmol/L, make it become the suspension of homogeneous in the NaCI solution, 4 ℃ of following filter paper filterings are removed lipoprotein precipitation wherein, collecting filtrate (contains the water-solubility protein component of Yolk immunoglobulin-WSF), obtains the content of Yolk immunoglobulin crude extract lyophilisation → mensuration Yolk immunoglobulin and activity → make Yolk immunoglobulin preparation
3. Chinese herbal medicine immunoglobulin preparation preparation:
3.1 earlier Chinese herbal medicine extracting powder and Yolk immunoglobulin powder are joined mixer mixing 1min according to 1: 1 ratio of Chinese herbal medicine extracting powder and Yolk immunoglobulin powder dry powder, add again, add 30～80% corn cob mixing 4min, promptly get composite feed additive.
3.2 utilize the ultrasonic extraction Chinese herbal medicine effective ingredients, merging filtrate, being concentrated into original liquid concentration is 1g/mL.With the Chinese herbal medicine liquid extract that makes and liquid egg yolk immunoglobulin according to mix at 1: 1 → content of Chinese herbal medicine immunoglobulin liquid → discontinuous sterilization → mensuration Yolk immunoglobulin and activity → allocate → bottle handle back → finished product (or making enteric Yolk immunoglobulin capsule or other dosage forms)
The specific embodiment two:
Raw material: Chinese herbal medicine extract, yolk, carrier
1. Chinese herbal medicine extract preparation: with Chinese herbal medicine with Radix Codonopsis, the Radix Pulsatillae, the Radix Astragali, Radix Isatidis, Herba Patriniae 1: 1: 1: 1: 1 mixed, utilize the broken back of micropowder flour to mix, utilize its effective ingredient of ultrasonic extraction again, the soak time before extracting is 60～90min.Ultrasonic frequency adopts 20～40kHz, ultrasonic Treatment 30min.After the filtration, make powder through low-temperature freeze-drying machine or spray drying.
2. yolk preparation:
After getting fresh high-immunity egg sterilization, manually beat eggs and get yolk or obtain egg yolk liquid by Ovum Gallus domesticus album/egg yolk separator.
3. Chinese herbal medicine and yolk compound formulation preparation:
Mix back → interpolation 20～80% defatted rice brans and 0.5% antioxidant → according to 1: 1 ratio of Chinese herbal medicine extract and yolk weight ratio by mixer mixing → sterilization → vacuum packaging → Chinese herbal medicine immunoglobulin powder product.
The specific embodiment three:
Raw material: Chinese herbal medicine extract, yolk egg white, carrier
1.1. Chinese herbal medicine extract preparation: with Chinese herbal medicine with Radix Sophorae Flavescentis, the Radix Pulsatillae, Rhizoma Coptidis, the Radix Astragali, Radix Isatidis, Herba Patriniae, Radix Glycyrrhizae 1: 1: 1: 1: 1: 1: 1 mixed, utilize the broken back of micropowder flour to mix, utilize its effective ingredient of ultrasonic extraction again, the soak time before extracting is 60～90min.Ultrasonic frequency adopts 20～40kHz, ultrasonic Treatment 30min.After the filtration, make powder through low-temperature freeze-drying machine or spray drying.
2. yolk egg white preparation:
After getting fresh high-immunity egg sterilization, beat eggs and get yolk Ovum Gallus domesticus album, remove shell breaking or obtain egg yolk liquid by Ovum Gallus domesticus album/egg yolk separator.
3. Chinese herbal medicine yolk egg white formulation preparation:
The Chinese herbal medicine extract and the yolk Ovum Gallus domesticus album that make are carried out proportioning → interpolation 20～60% white carbon blacks according to 1: 1 ratio, by the content and the activity → vacuum packaging → finished product of mixer mixing → sterilization → Chinese herbal medicine immunoglobulin powder → mensuration Yolk immunoglobulin
The specific embodiment four:
Raw material: Chinese herbal medicine little (ultra micro) pulverizing, Yolk immunoglobulin, carrier
1. Chinese herbal medicine little (ultra micro) is pulverized:
According to Chinese herbal medicine such as Radix Sophorae Flavescentis, the Radix Pulsatillae, Rhizoma Coptidis, the Radix Astragali, Radix Isatidis, Herba Patriniae, Herba Houttuyniae, Radix Glycyrrhizae 1: 1: 1: 1: 1: 1: 1: 1 mixed, utilize the broken back of micropowder flour to mix the preparation Chinese herbal medicine powder.
2. Yolk immunoglobulin preparation (employing water dilution method):
High-immunity egg with the dirt of clear water flush away eggshell surface, immerses in the 0.5% bromo geramine solution earlier, soaks the ethanol flushing of reuse 75%, the aseptic eggshell of breaking 10 minutes.Separate Ovum Gallus domesticus album and yolk, yolk adds 9 times of sterilized water dilutions, after the mixing of HCI adjusting pH5.2 diluent, in 4 ℃ of cold preservation 5h, centrifugal 1h under 4 ℃ of 1000g conditions, supernatant add saturated ammonium sulfate and the sodium sulfate precipitation of saltouing, centrifugal after, through low-temperature freeze-drying machine or spray drying, promptly get and slightly carry Yolk immunoglobulin.
3. Chinese herbal medicine immunoglobulin preparation preparation:
The Chinese herbal medicine extract and the Yolk immunoglobulin that make are carried out proportioning → interpolation 20～60% white carbon blacks according to 1: 1 ratio, by mixer mixing → sterilization → Chinese herbal medicine immunoglobulin powder → vacuum packaging → finished product
The specific embodiment five:
Raw material: Chinese herbal medicine little (ultra micro) pulverizing, yolk, carrier
1. Chinese herbal medicine extract preparation:
According to Chinese herbal medicine such as the Radix Pulsatillae, Flos Lonicerae, the Radix Pulsatillae, Radix Sophorae Flavescentis, Herba Plantaginis, the Radix Astragali, Herba Houttuyniae, Radix Glycyrrhizae 1: 1: 1: 1: 1: 1: 1: 1 mixed, utilize the broken back of micropowder flour to mix, utilize its effective ingredient of ultrasonic extraction again, the soak time before extracting is 60～90min.Ultrasonic frequency adopts 20～40kHz, ultrasonic Treatment 30min.After the filtration, make powder through low-temperature freeze-drying machine or spray drying.
2. yolk preparation:
After getting fresh high-immunity egg sterilization, manually beat eggs and get yolk or obtain egg yolk liquid by Ovum Gallus domesticus album/egg yolk separator.
3. Chinese herbal medicine and yolk compound formulation preparation:
Mix back → interpolation 20～80% defatted rice brans and 0.5% antioxidant → according to 1: 1 ratio of Chinese herbal medicine extract and yolk weight ratio by mixer mixing → sterilization → vacuum packaging → Chinese herbal medicine immunoglobulin powder product.
The specific embodiment six:
Raw material: Chinese herbal medicine little (ultra micro) pulverizing, yolk egg white, carrier
According to Chinese herbal medicine such as Radix Sophorae Flavescentis, the Radix Pulsatillae, Rhizoma Coptidis, Herba Plantaginis, Folium Isatidis 1: 1: 1: 1: 1: 1 mixed, utilize micronizing or atomizer to pulverize the back and mix, make Chinese herbal medicine powder.
2. yolk egg white preparation:
After getting fresh high-immunity egg sterilization, beat eggs and get yolk Ovum Gallus domesticus album, remove shell breaking or obtain egg yolk liquid by Ovum Gallus domesticus album/egg yolk separator.
3. Chinese herbal medicine yolk egg white formulation preparation:
The Chinese herbal medicine powder and the yolk Ovum Gallus domesticus album that make are carried out the soybean cover that proportioning → interpolation 20～60% is pulverized according to 1: 1 ratio, by mixer mixing → 4～5 minutes → Chinese herbal medicine of sterilization immunoglobulin powder → vacuum packaging → finished product.
Claims (7)
1, control diarrhea of pigs compound formulation adds the special yolk immunoglobulin and forms on Chinese herbal medicine base material basis, it is characterized in that so-called Chinese herbal medicine base material is to form with 2～14 kinds of raw material blending and mixings in Radix Codonopsis, Radix Sophorae Flavescentis, the Radix Pulsatillae, Rhizoma Coptidis, the Radix Astragali, Radix Isatidis, Fructus Forsythiae, Herba Patriniae, Poria, Flos Lonicerae, Herba Portulacae, Herba Plantaginis, Herba Houttuyniae, the Radix Glycyrrhizae etc.; So-called specific immune globulin is to utilize the unit price of preparations such as diarrhea of pigs Escherichia coli, clostridieum welchii and rotavirus, Transmissible gastroenteritis virus, epidemic diarrhea virus and bacillus perfringens or add inactivated vaccine immunity laying hen, its immune egg again through sterilization, separation purifying technique is prepared from, or by the serum immune globulin of preparations such as above-mentioned vaccine immunity mammal such as pig, rabbit; The solid or the liquid preparation that form according to certain proportioning and the compound mixing of carrier through the Chinese herbal medicine of pulverizing (or extraction) and specific immune globulin (or yolk, yolk egg white).Adopt oral, injection or method administration such as companion's feedstuff etc., diarrhea of pigs is had specially good effect, cure rate reaches more than 98%, improves growth speed of pigs more than 10%.
2, the preparation method of control diarrhea of pigs Chinese herbal medicine immunoglobulin compound formulation according to claim 1 is characterized in that compound formulation is powder, water preparation, granule or other dosage forms, can do feed additive and various medicament.
3, the preparation method of control diarrhea of pigs Chinese herbal medicine immunoglobulin compound formulation according to claim 1, it is characterized in that, the Chinese herbal medicine base material adopts and pulverizes, broken, the micronizing of micropowder, or the extract after pulverizing, and the ratio that adds in compound formulation is 1～99%.
4, the preparation method of control diarrhea of pigs Chinese herbal medicine immunoglobulin compound formulation according to claim 1, it is characterized in that, the special yolk immunoglobulin is for extracting, or the specific immune egg is directly beaten eggs after sterilizing, utilize the yellow and white or only use egg yolk, mix with Chinese herbal medicine in the right 3, the ratio that adds in compound formulation is 0.1～99% again.
5, the preparation method of control diarrhea of pigs Chinese herbal medicine immunoglobulin compound formulation according to claim 1, it is characterized in that, utilize the unit price of preparations such as diarrhea of pigs Escherichia coli, clostridieum welchii and rotavirus, Transmissible gastroenteritis virus, epidemic diarrhea virus and bacillus perfringens or add inactivated vaccine immunity laying hen, its immune egg adopts separation purifying technique to be prepared from.
6, the preparation method of control diarrhea of pigs Chinese herbal medicine immunoglobulin compound formulation according to claim 1 is characterized in that, adopts oral, injection, method administration such as companion's material etc., and diarrhea of pigs is had specially good effect.
7, carrier according to claim 1 is characterized in that, carrier comprises organic carrier and inorganic carrier.
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Assignee: Xuzhou Jinpai Pharmaceutical Co., Ltd. Assignor: Fang Xixiu|Wang Shouxuan|Wang Dongmei Contract fulfillment period: 2009.3.10 to 2019.3.9 contract change Contract record no.: 2009320000607 Denomination of invention: Production technique of immunoglobulin compounding agent capable of preventing and treating pig diarrhea License type: Exclusive license Record date: 2009.4.20 |
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