CN106260523A - A kind of feeding composite plant quintessence oil additive and feedstuff - Google Patents

Abstract

The invention discloses a kind of plants essential oil additive, including plants essential oil complex be coated wall material, described plants essential oil complex, by weight, including thyme oil 5-8 part, rosemary ethereal oil 3-5 part, Flos Caryophylli oil 2-5 part, Semen Myristicae oil 1-3 part, Oleum Cinnamomi 2-4 part.The present invention uses supercritical fluid CO₂Extraction extraction quintessence oil, material is without phase transition process, and technological process is simple, and extraction yield is high, and organic solvent-free remains, good product quality, non-environmental-pollution.The composite plant quintessence oil of the present invention has the physiological action of its uniqueness, the synergism of the most various compositions to make the using dosage of every kind of quintessence oil again seldom, the most both ensure that the feasibility on cost in turn ensure that effect that product is powerful.Play after adding in feedstuff with suitable dosage and effectively suppress the growth of harmful bacteria in feedstuff, extend the commodity price phase, promote feed intake, improve production performance and the biological function such as antioxidation and enhancing human body immunity.

Description

A kind of feeding composite plant quintessence oil additive and feedstuff

Technical field

The invention belongs to field of fodder, be specifically related to a kind of feeding composite plant quintessence oil additive and Feedstuff.

Background technology

The each state of our times is all reducing or is prohibitting the use of antibiotic.Viewpoint of Chinese food safety form is more Adding sternness, the livestock products of some outlets suffer damage because drug residue is too high or uses illegal drug Lose.Due to antibiotic use in a large number and abuse causes some Variation Pathogenic Bacterias so that originally Antibiotic effect is not notable, considers for bio-safety, it should reduces it and uses.Due to China Life-time service antibiotic, poultry form certain dependency to it, then find antibiotic Succedaneum be the thing that we compel now highly necessary to do.

Plants essential oil (Essential oil, EO) is the secondary metabolites in plant, one As with flower, leaf, branch, root, skin, natural gum and the fruit etc. of plant as raw material, pass through steamed Evaporate, squeeze and Herbivore aromatic substance that the method such as absorption extracts, be mainly composed of terpene Alkene, sesquiterpene and oxide thereof, the most also carbonyl compound, ester, alcohol etc..Quintessence oil has There is the strongest bactericidal function, especially gram positive bacteria and negative bacterium are had strong resisting Bacterium effect, is commonly applied to the aspects such as food additive, Pest control, sterilization, anticorrosion, mildew-resistant.

Traditional method of extraction of essential oil is mainly steam distillation and organic solvent extractionprocess.But Both approaches all also exists deficiency, the temperature that vapor distillation is required during extracting The highest, thermal decomposition and the hydrolysis of facile hydrolysis composition of thermographic compound in quintessence oil can be caused, The quintessence oil extracted must be driven off the moisture of entrained with, to prevent from going mouldy, extends the storage of product And the shelf-life, the product quality obtained is poor.Use organic solvent extraction can dissolve plant pigment And wax component, and cause dissolvent residual, poisonous, extraction time length, efficiency are low, it is impossible to from Dynamic operation.Therefore, how to reduce the destruction to effective ingredient, effectively extract plants essential oil, make The fragrance of natural plant essential oils is purer and product quality more preferably becomes and compared in the last few years Popular research topic.

It is described as the plants essential oil of " liquid golden " because there is antioxidation, the effect such as antibacterial, It is increasingly used for the fields such as medicine, cosmetics and food.Along with the development of science and technology, plant The advantages such as thing quintessence oil is natural with it, safe efficient become substitute antibiotics, effectively solve herding The various distresses faced in production and challenge, become the excellent product promoting healthy aquaculture. But limit its application in animal husbandry and have two reasons: one is because a lot of quintessence oil with having lacked not Working, display again to the side effect of toxicity during the dosage worked, two are because quintessence oil Expensive.

Summary of the invention

In order to solve the problems referred to above, the present invention provides a kind of feeding composite plant quintessence oil, and it is prepared Method and the feedstuff containing this composite plant quintessence oil.

A kind of plants essential oil additive, including plants essential oil complex be coated wall material, described in it Plants essential oil complex, by weight, including thyme oil 5-8 part, Herba Rosmarini Officinalis essence Oil 3-5 part, Flos Caryophylli oil 2-5 part, Semen Myristicae oil 1-3 part, Oleum Cinnamomi 2-4 part.

In one embodiment of the invention, described plants essential oil additive by weight, Including plants essential oil complex 10～40 parts, it is coated wall material 20～80 parts.

Wherein, described plants essential oil passes through supercritical CO₂Abstraction technique (SFE-CO₂) prepare.

Wherein, the described wall material that is coated is dry yeast cell.

Wherein said supercritical CO₂Concretely comprising the following steps of abstraction technique: the sample that will crush Loading in extraction column, the ethanol using 95% is as carrying agent, and addition is extraction kettle volume 5% (carries agent with ethanol and can increase solvent C O₂Polarity, simultaneously increase extract oils and fats Polarity, cause the increase of whole system separation factor, thus improve extraction yield).From steel Bottle CO out₂High-pressure pump boosting is entered after cold temperature groove (coolant is ethylene glycol) cools down, Open intake valve and make CO₂Fluid enters extracting system and contacts with raw material, and extracting pressure is by high-pressure pump Regulation (precision is ± 1%), extraction column is heated by heated constant temperature casing, and temperature passes through thermoelectricity The coupled control display received on panel, and regulated by it and show (precision for ± 0.1℃).Set CO₂Flow velocity, under predetermined temperature and pressure, raw material is fully and supercritical CO₂Contact, it is ensured that start to receive product after the 20～30min time of staying.Extract enters and divides Separating from tank, concurrently set the first separating still pressure and separation temperature, the second separating still divides Tripping power and separation temperature, dissolved the supercritical CO of quintessence oil with this understanding₂Pass through dropping valve Carry out throttling expansion and become atmosphere gas, extract and CO₂Separately, extract is by accepting bottle graft Receive, CO₂Gas is vented after wet flow indicator measures, and then calculates oil yield.

Wherein said CO₂For food stage, purity reaches 99.8%.

The computational methods of wherein said oil yield are: (material quantity-level of residue)/material quantity × 100%

Wherein, described thyme oil passes through supercritical fluid CO₂Extraction is planted from Herba thymi vulgaris Thing stem and leaf extracts, wherein, CO₂Flow is 20L/h, and extracting pressure is 20-25MPa, extraction Taking temperature and be 30-40 DEG C, extraction time is 2-4h, and the first separating still pressure is set to 9-10MPa, Separation temperature is set to 50 DEG C；Second separating still separating pressure is set to 4.5～5MPa, separates Temperature is set to 50 DEG C.The extraction process of described thyme oil is: choose fresh hundred In fragrant plant stem-leaf clean, naturally dry standby.Accurately weigh 200g to pulverize, cross 20 mesh, Extract with siftage.

Wherein, described rosemary ethereal oil passes through supercritical fluid CO₂Extraction is from natural fan repeatedly Perfume (or spice) extracts, wherein, CO₂Flow is 25L/h, and extracting pressure is 10-15MPa, extraction temperature Degree is for 35-40 DEG C, and extraction time is 2-4h, separates extract, the first separating still with Second separating still pressure is 8MPa, and the first separation temperature and the second separation temperature are 50 DEG C.

Wherein, described Flos Caryophylli oil passes through supercritical fluid CO₂Extraction is from Flos Caryophylli alabastrum Extract, wherein, CO₂Flow is 30L/h, and extracting pressure is 10-15MPa, and extraction temperature is 50-60 DEG C, extraction time 2-4h, extract is separated, the pressure of the first separating still is 8-10MPa, separation temperature is 30 DEG C, and the pressure of the second separating still is 5-8MPa, separates temperature Degree is 30 DEG C.

Wherein, described Semen Myristicae oil passes through supercritical fluid CO₂Extraction extracts from Semen Myristicae, Wherein, separating pressure is 30-35MPa, and separation temperature is 50-55 DEG C, CO₂Flow is 20L/h, Extraction time 2-4h, separates extract, and the pressure of the first separating still is 10MPa, Separation temperature is 30 DEG C, and the pressure of the second separating still is 8MPa, and separation temperature is 30 DEG C.

Wherein, described Oleum Cinnamomi passes through supercritical fluid CO₂Extraction extracts from Cortex cinnamomi japonici (Ramulus Cinnamomi), its In, separating pressure is 25-30MPa, and separation temperature is 40-50 DEG C, CO₂Flow is 35L/h, Extraction time 2-4h, separates extract, and the pressure of the first separating still is 5-10MPa, Separation temperature is 40 DEG C, and the pressure of the second separating still is 5-10MPa, and separation temperature is 40 DEG C.

The present invention also provides for the preparation method of described plants essential oil additive, and it includes walking as follows Rapid:

Composite plant quintessence oil mixes with being coated wall material, 65-70 DEG C, constant speed vibration 9-10h, anhydrous The quintessence oil of washing with alcohol wall material remained on surface, 30 DEG C of vacuum drying, to obtain final product.

The plants essential oil additive of the present invention can add in animal feed as additive, as in advance In batch mixing, concentrate feed or batch, therefore the present invention also provides for adding containing described plants essential oil Add the premix material of agent, concentrate feed or batch.Interpolation in basal diet, in piglet diet Amount for 100-200mg/kg, in addition in big swine rations be 100-150mg/kg.

The method have the benefit that

1. with tradition extraction of essential oil technique compared with, use supercritical fluid extraction have extraction and The dual function separated, material is without phase transition process, and technological process is simple, and extraction yield is high, without having Machine dissolvent residual, good product quality, non-environmental-pollution.

2. filtering out non-toxic essential oils, various quintessence oils have the physiological action of its uniqueness.Herba thymi vulgaris essence Oil: analgesic, diuretic, stimulant, immunostimulant, spasmolytic, aid digestion and breathing Nourishing agent, wherein effective ingredient thymol can improve digestion power, and antioxidation is antibacterial；Fan Repeatedly essential oil: antioxidant, antiinflammatory, analgesic, stimulant, aerofluxus, aid digestion and breathing Nourishing agent, effective ingredient cineole therein is improved digestibility, antioxidation, antibacterial Effect；Flos Caryophylli oil: antibacterial, analgesic, aerofluxus, the nourishing agent of stomach, therein have Effect ingredients eugenol has stimulation food calling, helps digest, antibacterial effect；Semen Myristicae oil: antiinflammatory, Analgesic, aerofluxus, diuretic, aid digestion nourishing agent；Oleum Cinnamomi: antibacterial, analgesic, Stimulant, digestion and the nourishing agent of respiratory system, effective ingredient cinnamic aldehyde therein has stimulation to lure Food, heightens digestion, antibacterial effect.

In the most exquisite formula, the synergism of various compositions makes the using dosage of every kind of quintessence oil again Seldom, the most both ensure that the feasibility on cost in turn ensure that effect that product is powerful.

4. promote appetite, increase and search for food: there is nature fragrant abnormal smells from the patient, olfactory nerves can be triggered With nervus glossopharyngeus, the appetite of stimulating animal, improve feed intake.

5, promote to absorb, improve digestibility: in composite plant quintessence oil, each composition all has itself Pharmacological effect, and each composition has its different work at the position that organism itself is different With, making salivation thing, gastric juice, pancreas liquid increases, thus improves digestion power and nutrient Absorption.

6, sterilization, diarrhea: quintessence oil molecule can improve cell with the double film of porate cells lipid The penetrance of wall, the cell wall transparent performance of increase causes filtering off Cell Component and increasing infiltration The destruction of effect, it is possible to destroy hereditary material, prevent bacteriological aftergrowth.

7, intestinal health colony balance is maintained: composite plant quintessence oil can be with acidifying in feedstuff Agent forms synergism, while killing harmful bacteria, stablizes the growth of profitable strain, improves Intestine microenvironment；Can accelerate to remove damage or the cell of necrosis on gastrointestinal tract, thus upset In the growth promoter cycle of internal body portion Enterozoa, control and Verminosis prevention.

8, composite plant quintessence oil has contained thymol to be the phenols chemical combination that non-oxidizability is the strongest Thing, can prevent the destruction of free radical, it is to avoid peroxidating, thus protects feedstuff to a certain extent Oils and fats and vitamin.

9, composite plant quintessence oil is a kind of natural biological preparation, can play with substitute antibiotics Promote growth, the effect of bacteriostasis and sterilization.

Detailed description of the invention

Following example are used for illustrating the present invention, but are not limited to the scope of the present invention.

The preparation of 1 one kinds of composite plant quintessence oils of embodiment

The extraction of thyme oil: choose fresh Herba thymi vulgaris plant stem-leaf and clean, naturally dry Standby.Accurately weigh 200g to pulverize, cross 20 mesh, extract with siftage.To crush Sample load in extraction column, the ethanol using 95% is as carrying agent, and addition is extraction kettle Long-pending 5%.From steel cylinder CO out₂After cold temperature groove cools down, enter high-pressure pump boosting, open Intake valve makes CO₂Fluid enters extracting system and contacts with raw material, and flow is 20L/h, sets extraction Pressure power is 25MPa, and extraction temperature is 40 DEG C, and raw material is fully and supercritical CO₂Contact, Extraction time is 4h, and Herba thymi vulgaris is carried out continuous extraction.Extract enters knockout drum to be carried out point From, the first separating still pressure is set to 10MPa, and separation temperature is set to 50 DEG C；Second point Being set to 5MPa from still separating pressure, separation temperature is set to 50 DEG C.Collect from two separating stills Liquid again through refined, obtain the blush quintessence oil that outward appearance is purer.By supercritical CO₂ The oil yield extracting quintessence oil is up to 4.2%.

The extraction of rosemary ethereal oil: pluck Herba Rosmarini Officinalis dry, stem, leaf, clean, go to cut weeds, Naturally dry.Accurately weigh the Herba Rosmarini Officinalis 200g after drying, pulverize 20 mesh sieves with pulverizer, Extract with siftage.The sample crushed is loaded in extraction column, then passes to supercriticality Under CO₂, flow is the pressurization of 25L/h, 15MPa program, 40 DEG C of temperature programmings, extraction 4h, separating pressure is 8MPa, and separation temperature is 50 DEG C, collects product, obtains yellow oil, Fragrant odour, oil yield is up to 3.5%.

The extraction of Flos Caryophylli oil: pluck the Flos Caryophylli alabastrum of scarlet maturation, removes bennet, accurately Weigh the Flos Caryophylli alabastrum 200g after drying, pulverized 20 mesh sieves with pulverizer, and carried with siftage Take.Being loaded in extraction column by the sample crushed, separating pressure is set to 15MPa, separation temperature It is 55 DEG C, CO₂Flow is 30L/h, and extract is separated, first by extraction time 3h The pressure of separating still is 9MPa, and separation temperature is 30 DEG C, and the pressure of the second separating still is 7MPa, Separation temperature is 30 DEG C, and oil yield is up to 20%, and Oleum Caryophylli is faint yellow supernatant liquid, easily It is dissolved in dehydrated alcohol.

The extraction of Semen Myristicae oil: Semen Myristicae is dried (control water content about 4%), accurately Weighing the Semen Myristicae 200g after drying, dry powder pulverizer was pulverized 40 mesh sieves, was carried with siftage Take.Being loaded in extraction column by the sample crushed, separating pressure is 32MPa, and separation temperature is 55 DEG C, CO₂Flow is 20L/h, and extract is separated by extraction time 2h, first point Pressure from still is 10MPa, and separation temperature is 30 DEG C, and the pressure of the second separating still is 8MPa, Separation temperature is 30 DEG C, and oil yield is up to 40.0%.

The extraction of Oleum Cinnamomi: pluck fresh dried and eat Cortex cinnamomi japonici (Ramulus Cinnamomi), is drying Cortex cinnamomi japonici (Ramulus Cinnamomi) at 40 DEG C Drying 4～5h in case, accurately weigh the Cortex cinnamomi japonici (Ramulus Cinnamomi) 200g after drying, dry powder pulverizer pulverized 20 Mesh sieve, extracts with siftage.Being loaded in extraction column by the sample crushed, separating pressure is 30MPa, separation temperature is 45 DEG C, CO₂Flow is 35L/h, and extraction time 4h, to extraction Thing separates, and the pressure of the first separating still is 9MPa, and separation temperature is 40 DEG C, second point Pressure from still is 6MPa, and separation temperature is 40 DEG C, and oil yield is up to 3.5%.

The preparation of composite essential oil: by the quintessence oil for preparing after purifying according to thyme oil 6 Part, oil of rosemary 4 parts, Flos Caryophylli oil 2 parts, Semen Myristicae oil 2 parts, Oleum Cinnamomi 3 parts Ratio is mixed to form composite essential oil, at least places 1 week.

Being coated of composite plant quintessence oil: precision weighs the dry yeast inactivated in right amount, puts into mill In mouth triangular flask, add composite plant quintessence oil 20 parts and dry yeast 40 parts, after adding a cover, put into perseverance In tepidarium agitator, temperature is set to 70 DEG C, constant speed (160r min^-1) vibration 10h after, Composite plant quintessence oil can enter inside yeast cells by diffusion effect, forms microcapsule. Decompression sucking filtration also reclaims the quintessence oil not wrapped into.The quintessence oil of absolute ethanol washing cell surface residual, 30 DEG C of vacuum drying, obtain composite plant essential oil microcapsules.The embedding of composite essential oil in microcapsule Rate is up to 60-70%.

The preparation of 2 one kinds of composite plant quintessence oils of embodiment

The extraction of thyme oil: choose fresh Herba thymi vulgaris plant stem-leaf and clean, naturally dry Standby.Accurately weigh 200g to pulverize, cross 20 mesh, extract with siftage.To crush Sample load in extraction column, the ethanol using 95% is as carrying agent, and addition is extraction kettle Long-pending 5%.From steel cylinder CO out₂After cold temperature groove cools down, enter high-pressure pump boosting, open Intake valve makes CO₂Fluid enters extracting system and contacts with raw material, and flow is 20L/h, sets extraction Pressure power is 20MPa, and extraction temperature is 35 DEG C, and raw material is fully and supercritical CO₂Contact, Extraction time is 4h, and Herba thymi vulgaris is carried out continuous extraction.Extract enters knockout drum to be carried out point From, the first separating still pressure is set to 9MPa, and separation temperature is set to 50 DEG C；Second separates Still separating pressure is set to 4.5MPa, and separation temperature is set to 50 DEG C.Collect from two separating stills Liquid again through refined, obtain the blush quintessence oil that outward appearance is purer, oil yield is 4.0%.

The extraction of rosemary ethereal oil: pluck Herba Rosmarini Officinalis scapus leaf, cleans, goes to cut weeds, natural Dry.Accurately weigh the Herba Rosmarini Officinalis 200g after drying, pulverized 20 mesh sieves with pulverizer, use Siftage extracts.The sample crushed is loaded in extraction column, then passes under supercriticality CO₂, flow is the pressurization of 25L/h, 10MPa program, and 38 DEG C of temperature programmings extract 3h, Separating pressure is 8MPa, and separation temperature is 50 DEG C, collects product, obtains yellow oil, abnormal smells from the patient Fragrance, oil yield is 3.6%.

The extraction of Flos Caryophylli oil: pluck the Flos Caryophylli alabastrum of scarlet maturation, removes bennet, accurately Weigh the Flos Caryophylli alabastrum 200g after drying, pulverized 20 mesh sieves with pulverizer, and carried with siftage Take.Being loaded in extraction column by the sample crushed, separating pressure is set to 10MPa, separation temperature It is 50 DEG C, CO₂Flow is 30L/h, extraction time 4h, and the pressure of the first separating still is 10MPa, Separation temperature is 30 DEG C, and the pressure of the second separating still is 6MPa, and separation temperature is 30 DEG C, Faint yellow clarification Oleum Caryophylli liquid, is soluble in dehydrated alcohol, and oil yield is 22.0%.

The extraction of the most cool oil of meat: Semen Myristicae is dried (control water content about 4%), accurately Weighing the Semen Myristicae 200g after drying, dry powder pulverizer was pulverized 40 mesh sieves, was carried with siftage Take.Being loaded in extraction column by the sample crushed, separating pressure is 35MPa, and separation temperature is 50 DEG C, CO₂Flow is 20L/h, extraction time 4h, and the pressure of the first separating still is 10MPa, Separation temperature is 30 DEG C, and the pressure of the second separating still is 8MPa, and separation temperature is 30 DEG C, Oil rate is 42.5%.

The extraction of Oleum Cinnamomi: pluck fresh dried and eat Cortex cinnamomi japonici (Ramulus Cinnamomi), is drying Cortex cinnamomi japonici (Ramulus Cinnamomi) at 40 DEG C Drying 4～5h in case, accurately weigh the Cortex cinnamomi japonici (Ramulus Cinnamomi) 200g after drying, dry powder pulverizer pulverized 20 Mesh sieve, extracts with siftage.Being loaded in extraction column by the sample crushed, separating pressure is 25MPa, separation temperature is 50 DEG C, CO₂Flow is 35L/h, extraction time 3h, first point Pressure from still is 10MPa, and separation temperature is 40 DEG C, and the pressure of the second separating still is 7MPa, Separation temperature is 40 DEG C, and oil yield is 3.0%.

The preparation of composite essential oil: by the quintessence oil for preparing after purifying according to thyme oil 3 parts, Oil of rosemary 3 parts, Flos Caryophylli oil 4 parts, Semen Myristicae oil 2 parts, the ratio that Oleum Cinnamomi is 4 parts It is mixed to form composite essential oil, at least places 1 week.

Being coated of composite plant quintessence oil: precision weighs the dry yeast inactivated in right amount, puts into mill In mouth triangular flask, add composite plant quintessence oil 30 parts and dry yeast 60 parts, after adding a cover, put into perseverance In tepidarium agitator, temperature is set to 65 DEG C, constant speed (160r min^-1) after vibration 9h, multiple Close plants essential oil and can enter inside yeast cells by diffusion effect, form microcapsule. Decompression sucking filtration also reclaims the quintessence oil not wrapped into.The quintessence oil of absolute ethanol washing cell surface residual, 30 DEG C of vacuum drying, obtain composite plant essential oil microcapsules.

Test example 1

1 EXPERIMENTAL DESIGN and feeding and management

Select the piglet 120 of 28 age in days about the 7.0kg that kind, parity and age in days are close. 4 groups are divided, often 3 repetitions of group by completely randomized design method, 10 pigs of each repetition, packet Time guarantee that the quantity often organizing test pig is close with average weight.Wherein matched group is planted without compound Thing quintessence oil, tests 1,2,3 groups and respectively adds 100mg/kg, 150mg/kg, 200mg/kg Composite plant quintessence oil.Test daily ration is according to ideal protein model, with reference to NRC2012's The basal diet of piglet nutrition standard preparation, ration ingredient is shown in Table 1.

Test in Bayan County, Harbin, Heilongjiang Province big north agriculture (hollow emerging) pig farm.Nursery house is adopted With slatted floor child care equipment, house temperature is 18-20 DEG C, and relative humidity is 50%-70%.Test Period respectively organizes piglet free choice feeding and drinking-water, cleans nursery house every day 1 time, every three days to guarantor Educate house spraying disinfection 1 time, use normal immunological program, test duration 30 days.

Table 1 basal diet composition and trophic level

2 Testing index

The mensuration of 2.1 growth performance indexs

Respectively at test formally start (28 age in days) and at the end of the 6:00 in early morning of (58 age in days) Carry out test pig before feeding weighing on an empty stomach in units of hurdle.Every day record dead wash in a pan pig quantity and Weight, calculates each stage average daily gain during testing, day feed consumption, feedstuff-meat ratio and diarrhea rate.

Average daily gain (BWG)=(terminating body weight-original body mass)/test natural law

Average daily ingestion amount (AFI)=feed intake/test natural law

Feedstuff-meat ratio (I/G)=average daily ingestion amount/average daily gain

Diarrhea rate (%)=diarrhoea piglet head number/(pig total head number × natural law) × 100%

2.2 respiratory system disease

Observe each test group pig with or without coughing, have a fever, have a stuffy nose, sneeze, air-breathing are abnormal, send out snore Eat several mouthfuls of new line mouths when sound, dog sitting posture ventral breathing or food deeply breathe or do ventilation action etc.

Respiratory tract abnormal symptom, records the head number that the piglet of each test group suffers from respiratory tract disease every day, Statistical correlation situation during off-test, the same diarrhea rate of computational methods.

3 data analysiss

With SAS9.0 statistical software, the data obtained is carried out variance analysis, use during significant difference DuncanShi method carries out multiple comparisons, and result of the test all represents with average ± standard deviation.

4 test results and analysis

4.1 piglet growth performance index determining result and analyses

The impact on piglet growth performance of the table 2 composite plant quintessence oil

Note: colleague's shoulder marking-up parent phase is with representing that difference is not notable (p ＞ 0.05), and letter is different represents significant difference (p ＜ 0.05)

Test group 2, the end body weight of test group 3 piglet are significantly higher than as can be seen from Table 2 Matched group (p ＜ 0.05), wherein the end body weight of test group 2 piglet improves than matched group respectively 10.18%；Average daily gain matched group to be significantly higher than (the p ＜ of test group 2 piglet 0.05)；Experimental group 1, the feedstuff-meat ratio of test group 2 piglet and diarrhea rate are the most all substantially less than comparison Group (p ＜ 0.05), wherein the diarrhea rate of test group 2 piglet reduces 59.9% than matched group.

The test group 1 of plant composite essential oil, test group 2, test group are added in result of the test explanation The production performance of 3 piglets is all better than matched group, because the effective ingredient in composite plant quintessence oil Eugenol and cinnamic aldehyde all have the effect of food calling, can suppress the bad smell in feedstuff, allow feedstuff Become more delicious, thus increase the palatability of daily ration, improve animal feed intake.Carvacrol and hundred In fragrant phenol, on the one hand can accelerate the renewal rate of intestinal villus surface-ripe enterocyte, simultaneously The maturation of digestive tract endodermis there is is facilitation, stimulates intestinal fine hair to grow, increase digestion and inhale Receive area；On the other hand gastrointestinal tract can be made excited, promote the secretion of the various digestive enzyme of intestinal, change The digestive and absorptive functions of kind animal, thus improve the digestive utilization ratio to feedstuff.Quintessence oil molecule can With the double film of porate cells lipid, improve the penetrance of cell wall, the cell wall transparent performance of increase Cause filtering off Cell Component and increasing chemosmotic destruction, it is possible to destroy hereditary material, anti- Only bacteriological aftergrowth, plays sterilization, anti-diarrhea effect.

The mensuration of 4.2 piglet health level and analysis

The impact on piglet respiratory tract disease of the table 3 composite plant quintessence oil

Note: colleague's shoulder marking-up parent phase is with representing that difference is not notable (p ＞ 0.05), and letter is different represents significant difference (p ＜ 0.05)

Test group 1, test group 2, the respiratory tract of test group 3 piglet as can be seen from Table 3 Sickness rate is significantly lower than matched group (p ＜ 0.05), the wherein morbidity of test group 1 piglet respiratory tract Rate is lower by 62.9% than matched group.Reason is in quintessence oil that bioflavonoids such as carvacrol can With humoral immunization and the cellular immune function of enhancing body, natural killer cell quantity can be improved.

Daily ration adds composite plant quintessence oil and can be effectively improved the feed intake of animal, improve growth Rate, addition when 100-200mg/kg, piglet terminate improve of weight ratio matched group 4.75-10.18%, and this composite plant quintessence oil can improve animal health level, reduces respiratory tract Disease and the sickness rate of diarrhoea.

The above is only the preferred embodiment of the present invention, it is noted that lead for this technology For the those of ordinary skill in territory, on the premise of without departing from the technology of the present invention principle, it is also possible to Making some improvements and modifications, these improvements and modifications also should be regarded as protection scope of the present invention.

Claims (10)

1. a plants essential oil additive, including plants essential oil complex and be coated wall material, its Being characterised by, described plants essential oil complex, by weight, including thyme oil 5-8 part, rosemary ethereal oil 3-5 part, Flos Caryophylli oil 2-5 part, Semen Myristicae oil 1-3 part, osmanthus Haze wax 2-4 part.

The preparation of composite plant quintessence oil the most according to claim 1, it is characterised in that press Weight portion meter, including plants essential oil complex 10～40 parts, is coated wall material 20～80 parts.

Plants essential oil additive the most according to claim 1 and 2, it is characterised in that institute The wall material that is coated stated is dry yeast cell.

Plants essential oil additive the most according to claim 1 and 2, it is characterised in that institute The thyme oil stated passes through supercritical fluid CO₂Extraction carries from Herba thymi vulgaris plant stem-leaf Take, wherein, CO₂Flow is 20L/h, and extracting pressure is 20-25MPa, and extraction temperature is 30-40 DEG C, extraction time is 2-4h, separates extract, and the first separating still pressure sets Being set to 9-10MPa, separation temperature is set to 50 DEG C；Second separating still separating pressure is set to 4.5～5MPa, separation temperature is set to 50 DEG C.

Plants essential oil additive the most according to claim 1, it is characterised in that described Rosemary ethereal oil passes through supercritical fluid CO₂Extraction extracts in natural Herba Rosmarini Officinalis, wherein, CO₂Flow is 25L/h, and extracting pressure is 10-15MPa, and extraction temperature is 35-40 DEG C, extraction The time of taking is 2-4h, separates extract, and the first separating still and the second separating still pressure are equal For 8MPa, the first separation temperature and the second separation temperature are 50 DEG C.

Plants essential oil additive the most according to claim 1 and 2, it is characterised in that institute State Flos Caryophylli oil by supercritical fluid CO₂Extraction extracts from Flos Caryophylli alabastrum, wherein, CO₂Flow is 30L/h, and extracting pressure is 10-15MPa, and extraction temperature is 50-60 DEG C, extraction Taking time 2-4h, separate extract, the pressure of the first separating still is 8-10MPa, point Being 30 DEG C from temperature, the pressure of the second separating still is 5-8MPa, and separation temperature is 30 DEG C.

Plants essential oil additive the most according to claim 1 and 2, it is characterised in that institute State Semen Myristicae oil by supercritical fluid CO₂Extraction extracts from Semen Myristicae, wherein, separates Pressure is 30-35MPa, and separation temperature is 50-55 DEG C, CO₂Flow is 20L/h, during extraction Between 2-4h, extract is separated, the pressure of the first separating still is 10MPa, separate temperature Degree is 30 DEG C, and the pressure of the second separating still is 8MPa, and separation temperature is 30 DEG C.

Plants essential oil additive the most according to claim 1 and 2, it is characterised in that institute State Oleum Cinnamomi by supercritical fluid CO₂Extraction extracts from Cortex cinnamomi japonici (Ramulus Cinnamomi), wherein, and separating pressure For 25-30MPa, separation temperature is 40-50 DEG C, CO₂Flow is 35L/h, extraction time 2-4h, Separating extract, the pressure of the first separating still is 5-10MPa, and separation temperature is 40 DEG C, The pressure of the second separating still is 5-10MPa, and separation temperature is 40 DEG C.

9. according to the preparation method of the plants essential oil additive described in any one of claim 1-8, It is characterized in that, comprise the steps:

Composite plant quintessence oil mixes with being coated wall material, 65-70 DEG C, constant speed vibration 9-10h, anhydrous The quintessence oil of washing with alcohol wall material remained on surface, 30 DEG C of vacuum drying, to obtain final product.

10. contain plants essential oil additive described in any one of claim 1-8 premix material, Concentrate feed or batch.