CN109805202A - Attractant plants essential oil product and its extracting method and broiler fodder product - Google Patents
Attractant plants essential oil product and its extracting method and broiler fodder product Download PDFInfo
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- CN109805202A CN109805202A CN201811628491.9A CN201811628491A CN109805202A CN 109805202 A CN109805202 A CN 109805202A CN 201811628491 A CN201811628491 A CN 201811628491A CN 109805202 A CN109805202 A CN 109805202A
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- essential oil
- parts
- plants essential
- oil product
- vitamin
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- 238000000034 method Methods 0.000 title claims description 32
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Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Fodder In General (AREA)
Abstract
The present invention relates to attractant plants essential oil products and preparation method thereof and broiler fodder product, and wherein the functional components of attractant plants essential oil product are the component of following mass percent: eugenol 3%-7%, thymol 2%-6%, citral 1%-5%, rutin 1%-5%, camellia phenol 1%-5%, other phenols 1%-4%；Broiler fodder product includes the attractant plants essential oil product and the feed ingredient that is adapted to it；The preparation method of attractant plants essential oil product includes, and chooses raw material and pulverizes and sieves, warm water immersions, ultrasonic wave added Enzymolysis, demulsification, extraction, drying and removes organic solvent step.Plants essential oil product of the invention and broiler fodder, full of nutrition, palatability is good, can significantly improve the feed intake of broiler chicken, while improving intestinal health, improves nutritive digestibility, and then promote growth of meat chicken.Plants essential oil preparation method provided by the invention environmental protection, efficiently, extraction of essential oil yield >=1.9%, total phenol aldehyde content is up to 16% or more.
Description
Technical field
The invention belongs to technical field of plant extraction, in particular to a kind of attractant plants essential oil product and its extracting method
With broiler fodder product.
Background technique
The broiler feeding period is short, the price of deed is high, raising and process automation are horizontal high, is that economic animal protein comes
Source, so that chicken consumption is in rapid growth trend.It is shown according to related data, the whole world possessed poultry from 1989 per capita
2.9kg increases to 11kg in 2016, wherein the Brazilian consumption figure of chicken per capita in 2016 is even more to have reached 43.8kg, it is the whole world
4 times of average level.The consumption figure of chicken has replaced beef to occupy second.
However, due to factors such as broiler feeding intensive degree height, stocking density height, if mismanagement, vulnerable to various
Pathogenic microorganism invasion, endanger broiler health, reduce production efficiency, aquaculture is caused to lose.It can be seen that exploitation has food calling
Effect and antibacterial effect can improve broiler health level and the novel fodder additive and feed that improve its growth performance to poultry
Industry is of great significance.
Plants essential oil has the characteristics that antipyretic analgesic, anti-inflammatory, antibacterial, anti-oxidant, lasting is lasting, in medicine, health care product, change
Cosmetic, additive etc. tool have been widely used.Currently, plants essential oil is also more next on livestock and poultry cultivation as feed addictive
It is more taken seriously, in terms of safeguarding animal health, promoting growth of animal and various raising animal, plants essential oil is equal
There is the effect of highly significant.Specific plant material is screened, prepares a kind of plants essential oil product with phagostimulating effect, and will
It is added in broiler fodder, for improving broiler chicken feed intake, and improves Intestine of Broiler health, application prospect is boundless.So
And current feed applications research work, it focuses mostly at the anti-oxidant aspect of antibacterial, in terms of taking into account to animal attractant effect, lacks
A whole set of is completely studied.
Summary of the invention
The purpose of the present invention is in order to solve the above problem, the present invention provides a kind of plant of the feeding grade with efficient phagostimulating effect
Object essential oil preparation and its extracting method and broiler fodder containing the plants essential oil product.
According to an aspect of the present invention, attractant plants essential oil product is provided, the function of the attractant plants essential oil product
Energy property ingredient is the component of following mass percent: eugenol 3%-7%, thymol 2%-6%, citral 1%-5%, rutin
1%-5%, camellia phenol 1%-5%, other phenols 1%-4%.
Wherein, the functional components of the attractant plants essential oil product are the component of following mass percent: eugenol
5.3%, thymol 3.5%, citral 2.4%, rutin 1.8%, camellia phenol 1.2%, other phenols 2.1%.
According to the second aspect of the invention, a kind of broiler fodder is provided, the broiler fodder include following mass fraction at
Part: 50-65 parts of corn, 15-30 parts of dregs of beans, 5-10 parts of expanded soybean, 1-3 parts of soybean oil, 0.5-1.5 parts of mountain flour, calcium monohydrogen phosphate
1.0-2.0 parts, 0.1-0.5 parts of salt, 0-0.2 parts of L-lysine, 0.1-0.3 parts of DL- METHIONINE, 0-0.1 parts of L-threonine, dimension
0.5-1.0 parts of raw element mineral substance premix and 0.01-0.02 parts of the attractant plants essential oil product.
Wherein, vitamin and minerals premix, which refers to, provides per kilogram mixed feed: 10000IU vitamin A, 2000IU dimension
Raw element D3, 20.0IU vitamin E, 3.0mg vitamin K3, 2.5mg vitamin B1, 0.4mg vitamin B6, 0.015mg vitamin
B12, 25.0mg niacin, 8.0mg pantothenic acid, 1.2mg folic acid, 600mg choline chloride, 20.0mg iron, 15.0mg copper, 80.0mg zinc,
80.0mg manganese, 1.5mg iodine, 0.30mg selenium.
Wherein, broiler chicken early period material (1-21d) includes the composition of following mass fraction: 58.55 parts of corn, dregs of beans 28.30
Part, 6.50 parts of expanded soybean, 1.80 parts of soybean oil, 1.45 parts of mountain flour, 1.70 parts of calcium monohydrogen phosphate, 0.25 part of salt, L-lysine
0.10 part, 0.25 part of DL- METHIONINE, 0.08 part of L-threonine, 1.0 parts of vitamin and minerals premix and the attractant plant essence
0.02 part of oil product.
Wherein, broiler chicken later period material (22-42d) includes the composition of following mass fraction: 61.22 parts of corn, dregs of beans 24.50
Part, 8.00 parts of expanded soybean, 3.00 parts of soybean oil, 0.80 part of mountain flour, 1.50 parts of calcium monohydrogen phosphate, 0.35 part of salt, DL- METHIONINE
0.12 part, 0.5 part of vitamin and minerals premix and 0.01 part of attractant plants essential oil product described.
According to the third aspect of the invention we, the extracting method of the attractant plants essential oil product is provided, comprising the following steps:
One of lemon grass (Cymbopogon citratus), thyme, basyleave and cloves or various plants raw material are crushed and screened into end；To plant material powder
Normal-temperature water is added and complex enzyme carries out Enzymolysis, wherein complex enzyme is the mixture of pectase and protease；To extracting solution according to
It is secondary be demulsified, extracted, collected organic layer；By organic layer drying, organic solvent is removed, obtains plants essential oil product.
Wherein, in Enzymolysis step, hydrolysis temperature is 45 DEG C -55 DEG C, and enzymatic hydrolysis pH value is 3.5-4.5, and enzymolysis time is
60-90min。
Wherein, in Enzymolysis step, the ratio of complex enzyme and substrate solution is 0.5-1.5g:1L, pectase and protease
Mass ratio is 1:1, and the enzyme activity of pectase is 0.8-5.0U/mg, and the enzyme activity of protease is 1800-2500U/mg.
Wherein, extracting method further includes positioned at the ultrasonic pretreatment step to pulverize and sieve between step and Enzymolysis step
Suddenly, ultrasonic power 400-1000W, ultrasonic time 15-30min.
Wherein, it is demulsified in extraction step, demulsifier is sodium chloride, and the additive amount of demulsifier is 50-100mg/kg；Extractant
For petroleum ether, extraction time of repose is 10-30min.
Wherein, extracting method further includes being located at the warm water soaking step to pulverize and sieve between ultrasonic pretreatment step,
Wherein the solid-liquid ratio of plant material and room temperature clear water is 1g:5-10mL, soaking time 2-10h.
Extracting method further includes the enzyme deactivation step between Enzymolysis and the extraction step that is demulsified, enzyme-removal temperature 85
DEG C, the enzyme deactivation time is 12min；In sifting step, the mesh number of sieve pore is 40-60 mesh.
Wherein, wherein in dry and removal step, drying mode is dry for anhydrous sodium sulfate；Except organic solvent mode is
By rotary evaporation under rotavapor under vacuum state with petroleum ether of going out.
In conclusion the present invention have it is following the utility model has the advantages that
1, of the invention a kind of plants essential oil product and broiler fodder, significantly improve the feed intake of broiler chicken, and effectively change
It has been apt to Intestine of Broiler health, has improved disease resistance.
2, a kind of broiler fodder of the invention, sorting is reasonable, and compatibility is good, and nutrition is balanced, absorption and utilization easy to digest, raises
Expect that utilization rate is high, meat chicken growth performance can be significantly improved, improves economic benefit of aquaculture.
3, using ultrasound-assisted enzymolysis extract method, using high specificity enzyme by cell wall constituent degrade or water
Solution destroys cell wall structure, increases permeability of cell membranes, while being aided with ultrasonication, substantially increase plants essential oil
Extraction efficiency and extract yield, shorten extraction time；And Extraction solvent is water, avoids a large amount of organic solvents and uses and arrange
Pollution problem is put, production cost is greatly reduced.
4, the present invention is by specified plant raw material in 45 DEG C of -55 DEG C of temperature, the pH environment and 0.5-1.5g:1L of 3.5-4.5
Liquid-to-solid ratio under conditions of Enzymolysis, with this condition, complex enzyme has sufficient enzymatic activity, by the cell wall of plant material
Destroy, and enhance permeability of cell membranes suitably, essential oil yield is made to remain at 1.9% or more, at the same make phenolic at
Dividing can mostly be discharged to the greatest extent, and the acquisition of functional components in the essential oil preparation of acquisition greatly improved, make essential oil preparation obtained
Middle total phenol aldehyde content is more than 16%.
5, the present invention is handled by ultrasound wave auxiliary enzyme method, cell wall is first carried out Mechanical Crushing by ultrasonic wave, then lead to
The albumen and soluble polysaccharide substance in cellular content and cell wall in complex enzyme for hydrolyzing plant material are crossed, auxiliary improves
The efficiency of enzymatic hydrolysis, achieve the purpose that energy consumption less, it is time-consuming less, enzyme dosage it is low, to reduce cost.
Specific embodiment
The illustrative embodiments of the disclosure are described in more detail below.Although showing showing for the disclosure in specification
Example property embodiment, it being understood, however, that may be realized in various forms the disclosure without should be by embodiments set forth herein
It is limited.It is to be able to thoroughly understand the disclosure on the contrary, providing these embodiments, and can be by the model of the disclosure
It encloses and is fully disclosed to those skilled in the art.
A kind of attractant plants essential oil product X1 of embodiment 1
Attractant plants essential oil product X1 includes the component of following mass percent: eugenol 3%, thymol 6%, lemon
Lemon aldehyde 1%, rutin 5%, camellia phenol 1%, other phenols 4%, surplus are terpene compound and other impurities.
A kind of attractant plants essential oil product X2 of embodiment 2
Attractant plants essential oil product X2 includes the component of following mass percent: eugenol 7%, thymol 2%, lemon
Lemon aldehyde 5%, rutin 1%, camellia phenol 5%, other phenols 1%, surplus are terpene compound and other impurities.
A kind of attractant plants essential oil product X3 of embodiment 3
Attractant plants essential oil product X3 includes the component of following mass percent: eugenol 5.3%, thymol
3.5%, citral 2.4%, rutin 1.8%, camellia phenol 1.2%, other phenols 2.1%, surplus be terpene compound and
Other impurities.
A kind of attractant plants essential oil product X4 of embodiment 4
Attractant plants essential oil product X4 includes the component of following mass percent: eugenol 3.4%, thymol
3.9%, citral 2.9%, rutin 2.1%, camellia phenol 1.1%, other phenols 3.3%, surplus be terpene compound and its
His impurity.
A kind of attractant plants essential oil product X5 of embodiment 5
Attractant plants essential oil product X4 includes the component of following mass percent: eugenol 2.5%, thymol
2.9%, citral 4.7%, rutin 1.1%, camellia phenol 1.0%, other phenols 3.8%, surplus be terpene compound and
Other impurities.
The extracting method of 6 attractant plants essential oil product X3 of embodiment
1) removal of impurities is impregnated: being selected lemon grass (Cymbopogon citratus), thyme, basyleave and cloves as plant material, will be cleaned, smashes it through
Lemon grass (Cymbopogon citratus), thyme, basyleave and the cloves of sieve (crossing 60 mesh) press 1:1:1:1 constitutive material source, and room temperature clear water, plant is added
The solid-liquid ratio of raw material and room temperature clear water is 1g:5mL, impregnates 8h, and the plant material after impregnating is collected by centrifugation；
2) room temperature clear water is rejoined in plant material after steeping, the feed liquid of plant material and room temperature clear water after immersion
Than for 1g:8mL, first through ultrasonic pretreatment, ultrasonic power 500W, ultrasonic 15min；After ultrasound, it is added by pectase
With the complex enzyme (1:1) of protease composition, the ratio of complex enzyme and substrate solution is 1.0g:1L, and in complex enzyme, the enzyme activity of pectase is
(under the conditions of 50 DEG C and pH value are 4.0,1min decomposes polygalacturonic acid and generates needed for 1 μm of ol galacturonic acid 3.0U/mg
Enzyme amount, as 1 enzyme activity unit), the enzyme activity of protease be 2000U/mg (under conditions of 37 DEG C and pH value 3.0,1min water
Solve enzyme amount, as 1 enzyme activity unit needed for casein generates 1 μ g tyrosine).It is 45 DEG C, digests pH value in hydrolysis temperature
Under conditions of 4.5,75min is digested, then 85 DEG C of enzyme deactivation 12min again.After Enzymolysis, centrifugation obtains enzyme-extracting solution；
3) NaCl that 60mg/kg is added in the resulting enzyme-extracting solution of step 2) is demulsified, and petroleum ether is added, quiet
Set isolated organic layer after 15min；
4) organic layer for obtaining step 3) is dry with anhydrous sodium sulfate, and rotates and steam under rotavapor under vacuum state
Hair removes organic solvent, obtains the plants essential oil product X3 of light yellow clear.
It extracts interpretation of result: according to the quality of plants essential oil, calculating extraction of essential oil yield, and analyze the phenol in plants essential oil
Aldehydes ingredient.It will test, calculate, the essential oil yield of plants essential oil product X3 made from this method is 2.1%, and the plants essential oil
The total phenol aldehyde content of product is 16.3%, higher than 10% or more of existing extractive technique.
The extracting method of 7 attractant plants essential oil product X4 of embodiment
1) removal of impurities is impregnated: selecting lemon grass (Cymbopogon citratus), thyme and basyleave as plant material, by removal of impurities, grinding and sieving (mistake
40 mesh) lemon grass (Cymbopogon citratus), thyme and basyleave press 1:3:1 constitutive material source, room temperature clear water is added, plant material is clear with room temperature
The solid-liquid ratio of water is 1g:10mL, impregnates 2h, and the plant material after impregnating is collected by centrifugation；
2) room temperature clear water is rejoined in plant material after steeping, the feed liquid of plant material and room temperature clear water after immersion
Than for 1g:5mL, first through ultrasonic pretreatment, ultrasonic power 400W, ultrasonic 30min；After ultrasound, it is added by pectase
With the complex enzyme (1:1) of protease composition, the ratio of complex enzyme and substrate solution is 0.5g:1L, and in complex enzyme, the enzyme activity of pectase is
0.8U/mg, the enzyme activity of protease are 1800U/mg.Under conditions of hydrolysis temperature is 50 DEG C, enzymatic hydrolysis pH value is 3.5, enzymatic hydrolysis
60min, then 85 DEG C of enzyme deactivation 12min again.After Enzymolysis, centrifugation obtains enzyme-extracting solution；
3) sodium chloride that 50mg/kg is added in the resulting enzyme-extracting solution of step 2) is demulsified, and petroleum ether is added,
Isolated organic layer after standing 10min；
4) organic layer for obtaining step 3) is dry with anhydrous sodium sulfate, and rotates and steam under rotavapor under vacuum state
Hair removes organic solvent, obtains the plants essential oil product X4 of light yellow clear.
It extracts interpretation of result: according to the quality of plants essential oil, calculating extraction of essential oil yield, and analyze the phenol in plants essential oil
Aldehydes ingredient.Be computed, the essential oil yield of plants essential oil product X4 made from this method be 1.9%, pair and the plants essential oil system
The total phenol aldehyde content of product is 16.7%, higher than 10% or more of existing extractive technique.
The extracting method of 8 attractant plants essential oil product X5 of embodiment
1) removal of impurities is impregnated: lemon grass (Cymbopogon citratus) is selected as plant material, will be cleaned, the lemon grass (Cymbopogon citratus) of grinding and sieving (crossing 50 mesh),
The solid-liquid ratio of addition room temperature clear water, lemon grass (Cymbopogon citratus) and room temperature clear water is 1g:8mL, impregnates 10h, and the plant original after impregnating is collected by centrifugation
Material；
2) room temperature clear water is rejoined in plant material after steeping, the feed liquid of plant material and room temperature clear water after immersion
Than for 1g:10mL, first through ultrasonic pretreatment, ultrasonic power 1000W, ultrasonic 15min；After ultrasound, it is added by pectin
The complex enzyme (1:1) of enzyme and protease composition, the ratio of complex enzyme and substrate solution are 1.5g:1L, in complex enzyme, the enzyme activity of pectase
For 5.0U/mg, the enzyme activity of protease is 2500U/mg.Under conditions of hydrolysis temperature is 55 DEG C, enzymatic hydrolysis pH value is 4, enzymatic hydrolysis
90min, then 85 DEG C of enzyme deactivation 12min again.After Enzymolysis, centrifugation obtains enzyme-extracting solution；
3) sodium chloride that 100mg/kg is added in the resulting enzyme-extracting solution of step 2) is demulsified, and petroleum is added
Ether stands isolated organic layer after 30min；
4) organic layer for obtaining step 3) is dry with anhydrous sodium sulfate, and rotates and steam under rotavapor under vacuum state
Hair removes organic solvent, obtains the plants essential oil product X5 of light yellow clear.
It extracts interpretation of result: according to the quality of plants essential oil, calculating extraction of essential oil yield, and analyze the phenol in plants essential oil
Aldehydes ingredient.It is computed, the essential oil yield of plants essential oil product X5 made from this method is 1.9%, and the plants essential oil product
Total phenol aldehyde content be 16.0%, higher than 10% or more of existing extractive technique.
Embodiment 9 expects Y1 the broiler chicken early period containing efficient phagostimulating effect plants essential oil
The broiler chicken early period expects (1-21d), is made of the raw material of following portions by weight:
58.55 parts of corn, 28.30 parts of dregs of beans, 6.50 parts of expanded soybean, 1.80 parts of soybean oil, 1.45 parts of mountain flour, phosphoric acid hydrogen
1.70 parts of calcium, 0.25 part of salt, 0.10 part of L-lysine, 0.25 part of DL- METHIONINE, 0.08 part of L-threonine, vitamin mineral
0.02 part of 1.0 parts of matter premix and plants essential oil product X3.
Wherein, vitamin and minerals premix, which refers to, provides per kilogram mixed feed: 10000IU vitamin A, 2000IU dimension
Raw element D3, 20.0IU vitamin E, 3.0mg vitamin K3, 2.5mg vitamin B1, 0.4mg vitamin B6, 0.015mg vitamin
B12, 25.0mg niacin, 8.0mg pantothenic acid, 1.2mg folic acid, 600mg choline chloride, 20.0mg iron, 15.0mg copper, 80.0mg zinc,
80.0mg manganese, 1.5mg iodine, 0.30mg selenium.
The broiler chicken early period material Y1's the preparation method comprises the following steps: corn, dregs of beans crushed 1.8mm aperture sieve, by formula weighing, and
It is proportionally added into expanded soybean, soybean oil, mountain flour, calcium monohydrogen phosphate, salt, L-lysine, DL- METHIONINE, L-threonine, dimension life
Plain mineral substance premix and plants essential oil product X3 are sufficiently mixed uniformly.
Broiler chicken later period of the embodiment 10 containing efficient phagostimulating effect plants essential oil expects Y2
The wean broiler chicken later period expects Y2 (22-42d), is made of the raw material of following weight parts:
61.22 parts of corn, 24.50 parts of dregs of beans, 8.00 parts of expanded soybean, 3.00 parts of soybean oil, 0.80 part of mountain flour, phosphoric acid hydrogen
1.50 parts of calcium, 0.35 part of salt, 0.12 part of DL- METHIONINE, 0.5 part of vitamin and minerals premix and plants essential oil product X3
0.01 part.
Wherein, vitamin and minerals premix, which refers to, provides per kilogram mixed feed: 10000IU vitamin A, 2000IU dimension
Raw element D3, 20.0IU vitamin E, 3.0mg vitamin K3, 2.5mg vitamin B1, 0.4mg vitamin B6, 0.015mg vitamin
B12, 25.0mg niacin, 8.0mg pantothenic acid, 1.2mg folic acid, 600mg choline chloride, 20.0mg iron, 15.0mg copper, 80.0mg zinc,
80.0mg manganese, 1.5mg iodine, 0.30mg selenium.
Broiler chicken later period material Y2's the preparation method comprises the following steps: corn, dregs of beans crushed 1.5-1.8mm aperture sieve, is claimed by formula
Weight, and it is proportionally added into expanded soybean, soybean oil, mountain flour, calcium monohydrogen phosphate, salt, DL- METHIONINE, vitamin and minerals premix
Material and plants essential oil product X3 are sufficiently mixed uniformly.
The raw material screening of 1 plants essential oil product of test example is tested
1) cloves, cortex cinnamomi, illiciumverum, wild marjoram, lemon grass (Cymbopogon citratus), peppermint, the coptis, scutellaria, basyleave, branch and thyme etc. are planted
Raw material crushes, and crosses 40-60 mesh, for use.
2) test fish management and domestication: (58cm × 58cm × 45cm) in aquarium is temporarily supported with commodity hard pellet diet
Test fish carries out feeding domestication, and control water temperature is maintained at 22 DEG C -24 DEG C, and pH value is between 6~8.Morning 9:30 and 4:00 in afternoon
It respectively feeds once, feeding rate is 2%-3%.It changes water 1 time daily, quantity of exchanged water is 30% or so.
3) preparation of bait ball: adding 2% plant material powder respectively in fish meal, and the starch being cooked with equivalent is mixed
It closes, is bundled into bait ball with hospital gauze, is sized so that fish can only sting and pecks, can not eat.4 bait balls are made in test every time, altogether
It is repeated 3 times.
4) formal test carries out in labyrinth, and labyrinth is made of organic glass, and chassis is octagon, is each divided into 17
A cell is communicated with diameter for the hole of 4cm, has the centrally located room of an outlet pipe, test fish can freely enter and leave in each room, because fish has
Therefore the habit countercurrently swum is respectively put into 1 branch water inlet pipe in each attracting room.After on-test, first make water flow in labyrinth
5min is recycled, the long 8-10cm of 6 urosomes or so tench is then arbitrarily selected in the test fish in aquarium for 24 hours of having stopped eating and is set
In in central compartment.Meanwhile in each attracting room, it is respectively put into the bait ball containing different plant materials, observes and records every point
Clock Nei tench enters the number of each attracting room and stings the number for pecking every kind of bait ball, records 120min altogether daily, records 2 days.
Every kind of plant material is as shown in table 1 to the test result of the phagostimulating effect of tench.
The phagostimulating effect of the different plant material Dui tenches of table 1 compares
As shown in Table 1, different plant materials to the phagostimulating effect of tench successively are as follows: lemon grass (Cymbopogon citratus) > thyme > basyleave >
Cloves > scutellaria > illiciumverum > coptis > cortex cinnamomi > wild marjoram > peppermint > branch.Wherein, lemon grass (Cymbopogon citratus), thyme, basyleave and fourth
Fragrant phagostimulating effect is apparently higher than other raw materials.Therefore, made with the one or more of lemon grass (Cymbopogon citratus), thyme, basyleave and cloves
For the raw material sources for extracting the efficient food calling plants essential oil of the preparation present invention.
The optimization of the optimal enzymatic hydrolysis condition of test example 2 is tested
Test is extracted based on the extracting method of the plants essential oil product X3 of embodiment 6, to solid-liquid ratio, enzymatic hydrolysis pH
Four value, hydrolysis temperature and enzymolysis time enzymatic hydrolysis condition factors are encoded (coding schedule is as shown in table 2), measure each enzymatic hydrolysis item
Influence of the part factor to the recovery rate of final plants essential oil product.Test result is as shown in table 3.
2 enzymatic hydrolysis condition design factor level code table of table
3 enzymatic hydrolysis condition of table extracts the influence of yield to plants essential oil
According to table 3, the extraction of essential oil rate of the plants essential oil as obtained by this test is generally up to 1.9% or more.Wherein,
Highly preferred enzymolysis and extraction condition is that solid-liquid ratio is 1g:8mL, and enzymatic hydrolysis pH value is 4.5, and hydrolysis temperature is 45 DEG C, enzymolysis time
For 75min, with this condition, the extraction yield of plants essential oil is up to 2.11%.
Influence experiment of 3 demulsifier of test example to plants essential oil yield
Test is extracted based on the extracting method of the plants essential oil product X3 of embodiment 6, and demulsifier is successively selected as
Sodium chloride, sodium carbonate, fatty alcohol and PSAF coagulant extract test.The quality for measuring plants essential oil, calculates each experiment
Extraction of essential oil yield, the results are shown in Table 4.
Influence of the different demulsifiers of table 4 to plants essential oil yield
According to table 4, demulsifier is a key factor for influencing to take plants essential oil yield, and wherein sodium carbonate is as broken
The recovery rate for the plants essential oil product that emulsion obtains is minimum, and the recovery rate of fatty alcohol and PSAF coagulant is placed in the middle, and sodium chloride mentions
Taking rate is highest.
The application of the broiler fodder of 4 product of plants essential oil containing attractant of test example
1.1 experimental designs and daily ration
Broiler fodder Y1 and Y2 obtained by the above embodiment of the present invention 9,10 search for food to broiler chicken with conventional broiler fodder
The influence of amount, the speed of growth, intestinal microflora compares test.
1 aa broiler chicken 360 is selected, 2 processing, 6 repetitions of each processing, 30 chickens of each repetition are randomly divided into
(being divided into three layers of cage raising of upper, middle and lower).Control group fed ordinary maize bean pulp type feed, embodiment group Broiler chicks front and back phase difference
Feed the feed of embodiment 9 and embodiment 10).Experimental period 42d.Control group daily ration used is that ordinary maize-bean pulp type broiler chicken is raised
(for commercially available broiler chickens mixed feed, primary raw material is corn, dregs of beans, soybean oil, salt, mountain flour, calcium monohydrogen phosphate, minerals dimension to material
Raw element premix, DL-lysine etc.), plants essential oil product is not added.
1.2 feeding management
Test is carried out according to AA broiler feeding standard, normal immunological, using free choice feeding and water way, is swept on time,
Keep pouity dwelling place sanitation and hygiene.
1.3 testing indexs and method
1.3.1 growth performance: weighing, ramming material after testing the 1st, 22,43 day morning empty stomach 12h calculate average body
Weight, average daily gain, average daily gain and feed consumption weight gain ratio.
1.3.2 intestinal microflora: in test the 22nd and the 43rd day, the male and female close to weight homogeneous was selected in each repetition
It chicken each 1, is dissected immediately after butchering, it is sterile to take caecum, it is put into aseptic plastic bag after the ligation of both ends, is immediately placed in -80 DEG C of guarantors
It deposits.0.5g or so intestinal segment content is weighed in aseptic operating platform in sterilizing dilution bottle, and enteron aisle is measured using coated plate counting mode
Interior Escherichia coli, C.perfringens, Bacillus acidi lactici and salmonella quantity.
2 results and analysis
Influence of 2.1 broiler fodders containing efficient phagostimulating effect plants essential oil to meat chicken growth performance
5 meat chicken growth performance contrast table of table
From the data in table 5, it can be seen that invention broiler fodder significantly improves being averaged for broiler trial 1-21d, 22-42d and 1-42d
Daily ingestion amount, especially phase improvement effect is clearly before the test；Meanwhile the test end for effectively increasing broiler chicken is heavy and average
Daily gain.This shows that the broiler fodder of the efficient phagostimulating effect plants essential oil of the present invention has significant phagostimulating effect, can significantly promote
Into growth of meat chicken.
Influence of 2.2 broiler fodders containing efficient phagostimulating effect plants essential oil to Intestine of Broiler microbial flora
6 broiler chicken faecal flora flora contrast table of table
| Project | Control group | Embodiment group |
| Bacillus acidi lactici (lg (CFU/g)) | 6.33 | 7.05 |
| Escherichia coli (lg (CFU/g)) | 7.08 | 6.36 |
| C.perfringens (lg (CFU/g)) | 3.02 | 2.76 |
| Salmonella (lg (CFU/g)) | 6.29 | 6.01 |
From the data in table 6, it can be seen that broiler fodder of the present invention significantly increases broiler chicken colonic contents compared with blank control group
Middle beneficial bacterium Bacillus acidi lactici quantity, and significantly reduce the quantity of harmful bacteria Escherichia coli, C.perfringens and salmonella.
The broiler fodder for showing that the present invention contains efficient phagostimulating effect plants essential oil has the effect of antibacterial, and it is micro- effectively to adjust enteron aisle
Biological flora balance, improves intestinal health.
In conclusion plants essential oil preparation method provided by the invention is easy to operate, environmentally friendly, efficient, cost is greatly reduced,
Extraction of essential oil yield >=1.9%, total phenol aldehyde content is up to 16% or more, much higher than the extraction separation of domestic and international existing plants essential oil
Technology.Plants essential oil product of the invention and broiler fodder, full of nutrition, palatability is good, can significantly improve the feeding of broiler chicken
Amount, while intestinal microflora balance is had adjusted, intestinal health is improved, and then promote growth of meat chicken.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
In the technical scope disclosed by the present invention, any changes or substitutions that can be easily thought of by anyone skilled in the art,
It should be covered by the protection scope of the present invention.Therefore, protection scope of the present invention should be with the protection model of the claim
Subject to enclosing.
Claims (10)
1. attractant plants essential oil product, which is characterized in that the attractant plants essential oil product is by lemon grass (Cymbopogon citratus), thyme, the moon
One of Gui Ye and cloves or a variety of are used as raw material, the complex enzyme formed by pectase and protease by Enzymolysis and
At；The functional components of the attractant plants essential oil product are the component of following mass percent: eugenol 3%-7%, hundred
In phenol 2%-6%, citral 1%-5%, rutin 1%-5%, camellia phenol 1%-5%, other phenols 1%-4%.
2. broiler fodder, which is characterized in that the broiler fodder includes the composition of following mass fraction:
50-65 parts of corn, 15-30 parts of dregs of beans, 5-10 parts of expanded soybean, 1-3 parts of soybean oil, 0.5-1.5 parts of mountain flour, calcium monohydrogen phosphate
1.0-2.0 parts, 0.1-0.5 parts of salt, 0-0.2 parts of L-lysine, 0.1-0.3 parts of DL- METHIONINE, 0-0.1 parts of L-threonine, dimension
Raw 0.5-1.0 parts and 0.01-0.02 parts of attractant plants essential oil product as described in claim 1 of element mineral substance premix；
Wherein, vitamin and minerals premix, which refers to, provides per kilogram mixed feed: 10000IU vitamin A, 2000IU vitamin
D3, 20.0IU vitamin E, 3.0mg vitamin K3, 2.5mg vitamin B1, 0.4mg vitamin B6, 0.015mg vitamin B12、
25.0mg niacin, 8.0mg pantothenic acid, 1.2mg folic acid, 600mg choline chloride, 20.0mg iron, 15.0mg copper, 80.0mg zinc,
80.0mg manganese, 1.5mg iodine, 0.30mg selenium.
3. broiler fodder as claimed in claim 2, which is characterized in that the broiler fodder include following mass fraction at
Part:
58.55 parts of corn, 28.30 parts of dregs of beans, 6.50 parts of expanded soybean, 1.80 parts of soybean oil, 1.45 parts of mountain flour, calcium monohydrogen phosphate
1.70 parts, 0.25 part of salt, 0.10 part of L-lysine, 0.25 part of DL- METHIONINE, 0.08 part of L-threonine, vitamin and minerals
1.0 parts and described 0.02 part of attractant plants essential oil product of premix.
4. broiler fodder as claimed in claim 2, which is characterized in that the composition including following mass fraction:
61.22 parts of corn, 24.50 parts of dregs of beans, 8.00 parts of expanded soybean, 3.00 parts of soybean oil, 0.80 part of mountain flour, calcium monohydrogen phosphate
1.50 parts, 0.35 part of salt, 0.12 part of DL- METHIONINE, 0.5 part of vitamin and minerals premix and the attractant plants essential oil
0.01 part of product.
5. the extracting method of attractant plants essential oil product as described in claim 1, which comprises the following steps:
One of lemon grass (Cymbopogon citratus), thyme, basyleave and cloves or various plants raw material are crushed and screened into end；
Normal-temperature water is added to plant material powder and complex enzyme carries out Enzymolysis, wherein the complex enzyme is pectase and albumen
The mixture of enzyme；
It is successively demulsified, extracted, collected organic layer to extracting solution；
By organic layer drying, organic solvent is removed, obtains the plants essential oil product.
6. extracting method as claimed in claim 5, which is characterized in that
In Enzymolysis step, hydrolysis temperature is 45 DEG C -55 DEG C, and enzymatic hydrolysis pH value is 3.5-4.5, enzymolysis time 60-90min.
7. the extracting method of attractant plants essential oil product as claimed in claim 6, which is characterized in that
Wherein, the ratio of complex enzyme and substrate solution is 0.5-1.5g:1L, and the mass ratio of pectase and protease is 1:1, pectase
Enzyme activity is 0.8-5.0U/mg, and the enzyme activity of protease is 1800-2500U/mg.
8. extracting method as claimed in claim 5, which is characterized in that
The extracting method further includes surpassing positioned at the ultrasonic pretreatment step to pulverize and sieve between step and Enzymolysis step
Acoustical power is 400-1000W, ultrasonic time 15-30min.
9. extracting method as claimed in claim 5, which is characterized in that
In the extraction step that is demulsified, demulsifier is sodium chloride, additive amount 50-100mg/kg；Extractant is petroleum ether, is extracted quiet
Setting the time is 10-30min.
10. extracting method as claimed in claim 8, which is characterized in that
The extracting method further includes positioned at the warm water soaking step to pulverize and sieve between ultrasonic pretreatment step, wherein planting
The solid-liquid ratio of raw material and room temperature clear water is 1g:5-10mL, soaking time 2-10h.
The extracting method further includes the enzyme deactivation step between Enzymolysis and the extraction step that is demulsified, enzyme-removal temperature 85
DEG C, the enzyme deactivation time is 12min；In sifting step, the mesh number of sieve pore is 40-60 mesh.
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Application publication date: 20190528 |