CN102715353A - Application of plant polyphenol compound during livestock and poultry culture - Google Patents
Application of plant polyphenol compound during livestock and poultry culture Download PDFInfo
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- CN102715353A CN102715353A CN2012101482426A CN201210148242A CN102715353A CN 102715353 A CN102715353 A CN 102715353A CN 2012101482426 A CN2012101482426 A CN 2012101482426A CN 201210148242 A CN201210148242 A CN 201210148242A CN 102715353 A CN102715353 A CN 102715353A
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Abstract
The invention relates to an application of a plant polyphenol compound during livestock and poultry culture, which belongs to the feed additive technical field. Aiming at unique chemical activity and physiological activity of plant polyphenol, the present invention proposes that plant polyphenol is used as the feed additive for improving health of livestock and poultry as well as raising the growth performance. The invention has the advantages that the plant polyphenol can replace an anti-oxidant, an antibacterial growth stimulator and an antiseptic which can promote the cells and tissue hyperplasia of animals, the plant polyphenol can be taken as a feed additive to add in a basic daily ration, so that the animal productivity performance can be improved, the animal immunity can be enhanced and the mortality can be reduced. Plant polyphenol is the natural extract product, and has no toxic and side effects during usage and has significant environmental protection meaning and good application prospect. The application provided by the invention opens up a novel application prospect for deep processing of plants, and opens up a new research direction for researches of antibacterial growth promoting antibiotic substitution.
Description
Technical field
The present invention relates to the plant polyphenol compounds as feed addictive improving livestock birds health and improving the application in its growth performance, belong to field of feed additive technology.
Background technology
Plant polyphenol; Claim tannic acid on the pharmacopeia again; It is a kind of polymer of phenol; Being distributed in widely in the leaf, bark, fruit, tree root of plant, especially in Salicaceae, Fagaceae, polygonaceae, the rose family, pulse family, Myrtaceae and the Rubiaceae of gymnosperm and dicotyledon, to be many, is the object of plant resources comprehensive utilization.For example in many coniferous barks, plant polyphenol content is up to 20%-40%, and only this has promptly formed in the whole world and has counted the renewable resource in hundred million tons.Plant polyphenol is closely bound up with human life; People have also absorbed plant polyphenol more or less in edible grain, vegetables, fruit and drink tea and fruit drink; And as the native compound of one type of biologically active, digestion, nutrition, health that they can the people exert an influence; Similarly, plant polyphenol is also closely related with the growth quality of livestock, crop disease and pest resisting and crop etc.Therefore, opinion is considered from the utilization and the Search green chemistry of renewable resource especially, and still from seeing with the mankind's relational angle, plant polyphenol all is the natural wide thing that one type of very worth people pays close attention to, research and development utilizes.
Plant polyphenol can divide three types: 1, Hydrolysable Tannins: according to the difference that produces the acid of polyhydric phenols hinge after the hydrolysis, Hydrolysable Tannins can be divided into: (1) gallotannin produces gallate (gallic acid) after the hydrolysis; (2) Ellagitannins produces the material that ellagic acid or other and six carboxyl biphenyl diacid have the source of students relation after the hydrolysis.Hydrolyzable tannins, is a class of phenolic acids and their derivatives with glucose, or a polyol glycoside bond or an ester bond by the formation of a compound; 2, condensed tannins (proanthocyanidins), is a class of catechin or a derivative thereof catechin gallate flavan-3 - ol compound with carbon - carbon bond in a compound formed by polymerization; 3, complex polyphenols, that is, a class of ellagic yellow monobutyl alkyl (eg narrow-leaf oak tannin, Ma oak elements, etc.).This type polyphenol molecule contains the construction unit of two types of hydrolysis class polyphenol and condensation class polyphenol simultaneously, has the characteristic of two types of polyphenol.
Plant polyphenol is initial main as the process hides tanning agent.Over nearly 30 years; Along with a series of chemisms such as the action rule of this type natural products and protein, polysaccharide, alkaloid, microorganism, enzyme, metal ion etc. and anti-oxidant, Mulberry Extract, derivative reaction are disclosed by people gradually; Plant polyphenol because of its anti-oxidant, anticancer anti-sudden change, antiviral, antimicrobial, slow down osteoporosis, strong tooth, reducing blood lipid and blood sugar etc. and have good effect aspect a lot, all be widely used in high-tech areas such as pharmacy, biochemistry, daily use chemicals, food and fine chemistry industries.
Plant polyphenol is considered to the anti-nutrient substance in the livestock-raising for a long time; A large amount of experiments show that plant polyphenol can make feed have puckery mouthful sensation; Thereby reduction feed intake; When the tannin content in the feed is higher, can influence the digestion of animal, reduce the nutritive value of food or feed protein, cellulose, starch and fat.Proteopeptic reduction easily forms stodgy compound with protein mainly due to tannin, and the reduction of other nutrient digestion rate is then main because tannin promotes the activity of enzyme such as cellulase to produce inhibition to corresponding digestion.
For preventing that countries in the world should be limited livestock is used antibiotic because of abuse antibiotic causes bacterium to develop immunity to drugs.From 2006, European Union's strict restriction or banned antibiotic use in the feed, Japan (2008) and Korea S (2011) also completely forbid in succession and in animal feed, add antibiotic.Most of countries and regions such as China, the U.S. increase the restriction kind of feeding antibiotic successively.Research and development can substitute antibiotic natural plant extracts feed addictive as active component plant polyphenol in more American-European companies.Domestic the discovery is used as the report that feed addictive improves livestock birds health and improves its growth performance with plant polyphenol.
Summary of the invention
The object of the present invention is to provide a kind of natural additive that can substitute antibiotic, improve the growth of animals or poultry performance, studied plant polyphenol as feed addictive for improving livestock birds health and improving the effect of its growth performance.
The present invention is directed to the chemical characteristic and the physiologically active of plant polyphenol, the domestic proposition first the plant polyphenol compounds as the application that can substitute antibiotic, improve the feed addictive of growth of animals or poultry performance.
Anti-nutritional position mainly is a condensed tannin in the plant polyphenol, and the Hydrolysable Tannins anti-oxidant action is very little.
The anti-oxidant action of plant polyphenol has dose dependent; The plant polyphenol of optimal dose can strengthen in vitro culture IEC-6 cell proliferation vigor; Improve the cell antioxygenic property, with plant polyphenol as feed addictive in the basal diet of livestock and poultry, again with its livestock and poultry of feeding; Can improve livestock birds health, and improve the growth of animals or poultry performance.
The plant polyphenol chemical reaction characteristic mainly contains following three aspects:
(1) a large amount of phenolic hydroxyl groups exist, and demonstrate very high antioxidation activity and radical scavenging activity.The effect of some plant polyphenols is strong than vitamin C and vitamin E, and has concertedness with it.
(2) plant polyphenol has resistance of wide spectrum (comprising the inhibition to filamentous fungi, yeast, bacterium, virus) to microorganism.As the secondary metabolite of plant, plant polyphenol can stop the invasion and attack of microorganism, comprises the tolerance and protection injury of raising to various pathogenic bacteria.
(3) recombination reaction that takes place with modes such as hydrophobic bond and hydrogen bonds of plant polyphenol and protein is its most important chemical property, is commonly called the convergence of polyphenol, and suitable plant polyphenol can be kept intestinal health, reduces diarrhea rate.
Advantage of the present invention:
(1) plant polyphenol has multi-efficiency in livestock and poultry cultivation, promptly can substitute feeding antibiotic, can reduce the use of anti-oxidant and anticorrisive agent again, thereby guarantees in livestock birds health cultivation, to practice thrift cost.
(2) plant polyphenol is the Nantural non-toxic extract, can not produce toxic and side effect when in feed, adding suitable dosage, and very high environment protection significance and good application prospects are arranged.
The invention discloses the application of plant polyphenol compounds in livestock and poultry cultivation, comprise the plant polyphenol that from different plants, extracts with different process.
The specific embodiment
Come further to set forth the present invention below in conjunction with concrete embodiment.Should be appreciated that these embodiment only are used to explain the present invention, and can not limit protection scope of the present invention.It will be understood by those skilled in the art that and down can make amendment with form or replace without departing from the spirit and scope of the present invention, but these modifications or replace all fall in protection scope of the present invention the details of technical scheme of the present invention.
The preparation technology of embodiment 1 plant polyphenol compounds
With Chinese chestnut involucre plant polyphenol kind compound technology is example, and the Chinese chestnut involucre is crushed to 60 orders, takes by weighing the 30% ethanol water mixing that 10g Chinese chestnut involucre powder adds 200mL; With ultrasonic Extraction 30min under 55 ℃, 500W power, centrifugal 15min under the 4000r/min condition, vacuum decompression revolve and steam leaching liquor (40 ℃, 0.1MP); Pump into the LSA-21 macroporous resin column that balance is good with concentrating the speed of leaching liquor, with the distilled water flushing pillar of 2 times of column volumes, use the flow velocity wash-out of 70% ethanol water more earlier with 2mL/min with 2mL/min; Collect 70% ethanol eluate; Vacuum rotary steam concentrates under 40 ℃, 0.1MP, is dried to powder, obtains the henna Chinese chestnut involucre of 1.368g extract powder; The yield of extract is 13.68%, and wherein the content of polyphenol is 74.90%.
Test Example 1 plant polyphenol promotes IEC-6 cell proliferation and improves its antioxygenic property test
One, test material
The IEC-6 cell line is available from China consonance medical sci-tech university cell holding room.
Test plant polyphenol compound: get the Chinese chestnut involucre extract 10mg of embodiment 1 preparation, after the dissolving of 5mL DMEM complete culture solution, through Φ 0.22 μ m membrane filtration, 4 ℃ of preservations.
Two, test method
The IEC-6 cell confluency reaches 95% when above, adds the DMEM complete culture solution after the pancreatin with 0.25% digests.After carrying out the calculating of cell count and cell viability under the inverted microscope, the adjustment cell concentration is 1 * 10
5Individual/mL, add 90 μ L cell suspensions respectively to 96 porocyte culture plates, test group: add 10 μ .L100 μ g/mL plant polyphenol compounds again, control group adds 10 μ LDMEM complete culture solutions.In 37 ℃, 5%CO
2Cell culture incubator 44h, every hole adds the MTT liquid of 10 μ L 5mg/mL, continues down to add 100 μ L DMSO liquid mixings behind the cultivation 4h at 37 ℃, detects the OD value in the 570nm place.IEC-6SOD, GSH-Px vigor and MDA Determination on content are carried out according to the kit specification.
All data are represented with Mean ± SD.Adopt SPSS 11.0 statistical softwares to carry out the data and the one-factor analysis of variance and multiple ratio.
Three, result of the test
The plant polyphenol compounds is seen table 1 to the influence of IEC-6 cell proliferation and anti-oxidant index; Compare with control group; The plant polyphenol compounds can significantly improve IEC-6 cell proliferation vigor and SOD, GSH-Px vigor; Reduce MDA content, explain that the plant polyphenol compounds can promote the growth of IEC-6 cell, and improve its antioxygenic property.
The influence of table 1 plant polyphenol compounds on cell proliferation and anti-oxidant index
Annotate: the different letter representation significant difference of same row P＜0.05.
Test Example 2: the plant polyphenol compounds is to the growth of meat chicken Effect on Performance
One, test material and method
100 1 age in days AA chick carry out immunity by the normal immune programme for children of fryer.Freely get food and drinking-water.2 weeks before test, according to standard brood the practice at indoor heating to reach required room temperature.At the 14th day, chick is weighed the Random assignment and the 2 kinds of daily rations of feeding: control group fed basal diet (table 2), test group is added embodiment 1 gained plant polyphenol compounds in basal diet feeding dorking in the ratio of 0.03wt%.Every kind of daily ration is established 3 repetitions (15 chicken/circles).Each circle (1.7 square metres) is covered with rice husk and wood chip (weight ratio 50: 50) as bedding and padding.Every chicken at the chicken wings mark to assert identity.6 weeks of test duration.Write down the death rate every day, weekly every chicken body weight of weighing and each circle feed feed intake.Calculate average daily gain, daily ingestion amount, feedstuff-meat ratio and death rate.
Table 2 basal diet composition and chemical composition
| Project | Quantity |
| Composition (%) | |
| Corn | 32.50 |
| Wheat | 20.00 |
| Soya-bean cake (crude protein 44%) | 36.40 |
| Animal tallow | 6.10 |
| Calcium carbonate | 1.70 |
| Dicalcium Phosphate | 1.30 |
| Sodium chloride | 0.19 |
| The DL-methionine | 0.19 |
| L-lysine | 0.12 |
| Phytase | 0.10 |
| Vitamin-mineral substance premix 1 | 5.00 |
| Chemical composition analysis (% material) | |
| Dry | 87.50 |
| Crude protein | 22.90 |
| Crude fat | 14.20 |
| Crude fibre | 2.30 |
| Ash content | 8.40 |
| Metabolizable energy 2(MJ/kg) | 12.34 |
2Add by every kg daily ration: vitamin A, 8000IU; Cholecalciferol, 2000IU; Vitamin B1,1.5mg; Riboflavin, 3.0mg; Vitamin B6,1.5mg; Cobalamin, 15 μ g; DL-α-D-, 75IU; Niacin usp, 25.0mg; D-pantothenic acid, 8.0mg; Choline Chloride, 500.0mg; Cobalt, 0.2mg; Iron, 30.0mg; Iodine, 1.4mg; Magnesium, 80mg; Copper, 1.5mg; Zinc, 30.0mg.
3Become to be grouped into table based on NRC (1994).
Two, result of the test
The plant polyphenol compounds is seen table 3 to 14-56 age in days growth of meat chicken performance impact.Use the average daily gain of the test group of plant polyphenol compounds of the present invention to be significantly higher than control group; Under the suitable situation of feed intake; The test group feedstuff-meat ratio is significantly higher than control group, test simultaneously show add behind the Chinese chestnut involucre extract of the present invention fryer extremely wash in a pan low, the rate of animals delivered to the slaughter-house is high.So utilize natural additive of the present invention can shorten marketing time, fast growth, chicken crowd turnaround speed is fast, and the feedstuff-meat ratio of test group is significantly less than control group, can save feed.
Table 3 plant polyphenol compounds is to 14-56 age in days growth of meat chicken Effect on Performance
| Divide into groups | Average daily gain (g) | Average feed intake (g) | Feedstuff-meat ratio | The rate of animals delivered to the slaughter-house (%) |
| Control group | 64.22±1.93 b | 138.20±4.91 | 2.14±0.03 a | 91.8 |
| Test group | 67.97±0.69 a | 139.34±2.54 | 1.95±0.04 b | 95.3 |
Annotate: the different letter representation significant difference of same row P＜0.05.
Test Example 3: the plant polyphenol compounds is to the fattening INFLUENCE OF YUCCA EXTRACT
One, test material and method
According to 60 of healthy Da Bai hybridization growing and fattening pigs about the principle selection body weight 30kg of " parity unanimity, body weight approaching, public female half and half "; Be divided into 2 groups at random, be respectively control group and test group, every group of 3 repetitions; Each repeats 10 pigs; Flat the supporting in house cement flooring, closed hurdle, it is identical that each organizes stocking density, free choice feeding with freely drink water.Add the embodiment 1 gained plant polyphenol compounds except that test group ratio in 0.03wt% in basal diet, other feeding and management conditions are in full accord.Preliminary trial period 7d, 90 days experimental periods.Write down diarrhoea quantity every day, weekly every pig body weight of weighing and each circle feed feed intake.Calculate average daily gain, daily ingestion amount, feedstuff-meat ratio and diarrhea rate.
Basal diet: prescription is according to the nutritional need preparation of pig, and main component is unit by weight percentage: corn 64%, inferior powder 5.6%, dregs of beans 22%, fish meal 3%, calcium bicarbonate 0.6%, stone flour 1%, whey powder 3%, vitamin and minerals additive 0.45%.
Two, result of the test
The plant polyphenol compounds is seen table 2 to the fattening INFLUENCE OF YUCCA EXTRACT, can find out, the daily gain of the test group of employing natural additive of the present invention is apparently higher than control group; And feedstuff-meat ratio and diarrhea rate are lower than control group; So the shortening marketing time is saved feed, reduces cost.
Table 4 plant polyphenol compounds is to the fattening INFLUENCE OF YUCCA EXTRACT
| Divide into groups | Average daily gain (g) | Average feed intake (g) | Section's meat ratio | Diarrhea rate (%) |
| Control group | 558.25±10.67b | 1875.74±20.23 | 3.36±0.23a | 20 |
| Experimental group | 682.83±22.01a | 1904.27±16.92 | 2.79±0.17b | 12 |
Claims (1)
1. the application of plant polyphenol compounds in livestock and poultry cultivation.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109393151A (en) * | 2018-04-20 | 2019-03-01 | 北京农学院 | A kind of feed addictive can be improved livestock and poultry production performance |
| CN110050906A (en) * | 2019-01-28 | 2019-07-26 | 广东海洋大学 | Hydrolysable Tannins is improving the application in prawn growth performance and gut of shrimp bacterial abundance |
| CN111134245A (en) * | 2020-01-19 | 2020-05-12 | 中国林业科学研究院林产化学工业研究所 | Valonea extract feed additive for broiler chickens and application thereof |
| CN111387368A (en) * | 2014-05-30 | 2020-07-10 | 嘉吉公司 | Method of feeding animals |
| CN111602751A (en) * | 2020-06-23 | 2020-09-01 | 广东合正元生物科技股份有限公司 | Complete formula feed for chickens |
| CN112568329A (en) * | 2020-12-15 | 2021-03-30 | 广州立达尔生物科技股份有限公司 | Myrtle polyphenol dispersed zinc oxide particles, and preparation method and application thereof |
| CN114041531A (en) * | 2021-11-30 | 2022-02-15 | 中国农业大学 | Ellagic acid feed additive and application thereof |
| CN114391605A (en) * | 2022-01-13 | 2022-04-26 | 肇庆市佰仕科生物科技有限公司 | Novel preparation method and application of plant polyphenol protein for feed |
| CN114423297A (en) * | 2019-06-17 | 2022-04-29 | 英派克斯有限公司 | Use of a composition for improving slaughter and/or meat quality parameters |
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Cited By (11)
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| CN111387368A (en) * | 2014-05-30 | 2020-07-10 | 嘉吉公司 | Method of feeding animals |
| CN111387368B (en) * | 2014-05-30 | 2024-02-02 | 嘉吉公司 | Method for feeding animals |
| CN109393151A (en) * | 2018-04-20 | 2019-03-01 | 北京农学院 | A kind of feed addictive can be improved livestock and poultry production performance |
| CN110050906A (en) * | 2019-01-28 | 2019-07-26 | 广东海洋大学 | Hydrolysable Tannins is improving the application in prawn growth performance and gut of shrimp bacterial abundance |
| CN110050906B (en) * | 2019-01-28 | 2022-08-26 | 广东海洋大学 | Application of hydrolyzed tannin in improving prawn growth performance and prawn intestinal microbial abundance |
| CN114423297A (en) * | 2019-06-17 | 2022-04-29 | 英派克斯有限公司 | Use of a composition for improving slaughter and/or meat quality parameters |
| CN111134245A (en) * | 2020-01-19 | 2020-05-12 | 中国林业科学研究院林产化学工业研究所 | Valonea extract feed additive for broiler chickens and application thereof |
| CN111602751A (en) * | 2020-06-23 | 2020-09-01 | 广东合正元生物科技股份有限公司 | Complete formula feed for chickens |
| CN112568329A (en) * | 2020-12-15 | 2021-03-30 | 广州立达尔生物科技股份有限公司 | Myrtle polyphenol dispersed zinc oxide particles, and preparation method and application thereof |
| CN114041531A (en) * | 2021-11-30 | 2022-02-15 | 中国农业大学 | Ellagic acid feed additive and application thereof |
| CN114391605A (en) * | 2022-01-13 | 2022-04-26 | 肇庆市佰仕科生物科技有限公司 | Novel preparation method and application of plant polyphenol protein for feed |
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