CN110623158A - Preparation method of wormwood feed for preventing African swine fever - Google Patents
Preparation method of wormwood feed for preventing African swine fever Download PDFInfo
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- CN110623158A CN110623158A CN201911004521.3A CN201911004521A CN110623158A CN 110623158 A CN110623158 A CN 110623158A CN 201911004521 A CN201911004521 A CN 201911004521A CN 110623158 A CN110623158 A CN 110623158A
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- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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Abstract
The invention discloses a preparation method of an wormwood feed for preventing African swine fever, which comprises the steps of preparing dreg-free liquid juice and a nutrient mixed solution, fermenting main nutrient substances, and drying and granulating, wherein the pig feed can effectively prevent live pigs from infecting the African swine fever, is rich in nutrition and good in palatability, effectively improves the immunity of the live pigs, enhances the disease resistance, balances the nutrition and has no chemical medicine residue; meanwhile, the preparation method has the advantages of low material cost, easily obtained raw materials, simple process, high practical value and good application prospect.
Description
Technical Field
The invention relates to the technical field of agricultural cultivation, and particularly relates to a preparation method of an wormwood feed for preventing African swine fever.
Background
On one hand, pork is closely related to daily life of people and is the most common meat on dining tables, and with the increasing improvement of living standard of people and the continuous expansion of the scale of breeding industry, the requirements of people on the quality of pork are higher and higher. However, in many pig farms, the pork quality is poor due to breeding species, feeding feed, breeding method, breeding environment, and the like, such as the pork has a lot of water exudation, and is white or dark in color. The reduction of pork quality can affect the nutritional value and the edible value of pork, in order to improve the pig raising benefit and ensure the healthy and continuous development of the pig raising industry, the pork quality must be greatly improved, the feed additive has practical significance for improving the pork quality problem, the current pig feed industry in China rapidly develops and intensifies the rapid development trend, and in the existing breeding industry, in order to improve the disease resistance of livestock, ensure the normal growth speed and reduce the death rate, antibiotic growth promoters, antibacterial and antiparasitic drugs and the like are generally used as the feed additive; however, chemical drugs such as antibiotics added to the feed cannot be completely digested and eliminated from the poultry or livestock after 2-3 months of ingestion, and if the poultry or livestock are slaughtered and eaten before the drug elimination period, the antibiotics or chemicals remaining therein cause the immunity of the eaters to decline. Especially antibiotics (antibiotics) are chemical substances which are generated by microorganisms or higher animals and plants in the living process, have a class of secondary metabolites with anti-pathogen or other activities and can interfere with other cell development functions. The antibiotics have growth promoting effect on livestock and poultry, and obtain obvious economic benefit. The abuse of these antibiotics can lead to the growth of certain superbacteria in humans, so-called "monster diseases", which are not cured by current medicine.
On the other hand, African Swine Fever (ASF) is a Swine disease caused by acute and filterable viruses with high febrile infectivity, and is characterized in that the disease process is short, but the death rate is up to 100%, the clinical manifestations of sick pigs are fever, skin cyanosis, obvious bleeding of lymph nodes, kidneys and gastrointestinal mucosa, how to effectively strengthen the physique of live pigs and how to improve the prevention and resistance effects of the live pigs on the viruses is a topic worthy of research.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide a wormwood pig feed and a preparation method thereof, which are used for solving the problems.
The technical scheme adopted by the invention for solving the technical problems is as follows:
the invention provides a preparation method of wormwood feed for preventing African swine fever, which is characterized by comprising the following steps:
a. putting 15-25 parts of folium artemisiae argyi, 2-4 parts of radix isatidis, 2-4 parts of liquorice, 3-5 parts of radix astragali, 1-3 parts of folium isatidis and 2-4 parts of herba violae into water, heating to 90-100 ℃, cooling the decocted liquid, and filtering by using a filter screen to obtain residue-free liquid A for later use;
b. 5-9 parts of vitamin additive, 4-6 parts of compound mineral, 0.5-0.7 part of amino acid, 0.7-0.9 part of calcium hydrophosphate, 0.2-0.4 part of ferric sulfate and 0.1-0.3 part of trace element are put into water with the temperature of 20-30 ℃ for mixing to obtain mixed solution B;
c. mixing and crushing 5-7 parts of marigold root, 1-3 parts of pepper, 1-3 parts of saponin seeds and 4-6 parts of mint leaf, drying at 50-60 ℃, and performing Soxhlet extraction to obtain a mixture C;
d. crushing 20-30 parts of wheat bran, 10-20 parts of sorghum, 1-3 parts of carrots, 5-7 parts of rice bran, 5-7 parts of fruit meal and 10-20 parts of corns, mixing with 1-2 parts of fish meal, 1-2 parts of raw eggs and 1-2 parts of fish oil, and uniformly stirring to obtain a mixture D for later use;
e. mixing and stirring the mixture C and the mixture D according to the weight ratio of 1:10-16, adding yeast fermentation liquor and bacillus fermentation liquor, uniformly stirring, carrying out sealed fermentation at 40-45 ℃ for 3-6 days, adding the residue-free liquid juice A, uniformly stirring, and carrying out sealed fermentation at 55-60 ℃ for 20-25 days to obtain a fermented product;
f. mixing the mixed solution B with the fermented product according to the weight ratio of 2: 25-35, adding the spirulina powder, the sucrose powder, the maltodextrin, the flavoring agent and the distilled water into the mixture, stirring for 20-30min at the rotating speed of 200 plus 220r/min, drying and granulating to obtain the finished product.
Preferably, the vitamin additive comprises 0.5-0.9 part of vitamin A, 61.5-2.9 parts of vitamin B, 1.3-3.7 parts of vitamin D and 0.8-2.3 parts of vitamin K.
Preferably, the solvent of Soxhlet extraction is chloroform, the extraction temperature is 75-80 ℃, and the extraction time is 5-7 h.
Preferably, the yeast fermentation broth of step b is obtained by fermenting and culturing yeast at 28-29 deg.C for 3-5 days.
Preferably, the bacillus fermentation liquid of the step b is obtained by fermenting and culturing bacillus at the temperature of 33-35 ℃ for 4-6 days.
Preferably, the bacillus is any one or a mixture of bacillus subtilis, bacillus cereus and bacillus curvatus.
Preferably, the amino acid is one or more of methionine, glutamic acid, lysine, arginine, glutamine and glycine.
Preferably, the composite mineral of step b is formed by mixing iron salt, copper salt, manganese salt, zinc salt, selenium salt, cobalt salt, iodine salt and magnesium salt.
Preferably, the preparation method of the spirulina powder comprises the following steps:
cleaning fresh spirulina, cutting, pulverizing in a pulverizer, spray drying for 1 ~ 3h, sieving with 80 ~ 200 mesh sieve, and microwave sterilizing for 1 ~ 3min to obtain the spirulina powder.
Preferably, the spirulina powder, the sucrose powder, the maltodextrin and the flavoring agent are added in amounts of 6%, 3%, 2% and 1% by weight of the mixture in the step respectively.
Preferably, the preparing step comprises the steps of:
a. putting 20 parts of folium artemisiae argyi, 3 parts of radix isatidis, 3 parts of liquorice, 4 parts of radix astragali, 2 parts of folium isatidis and 3 parts of herba violae into water, heating to 95 ℃, cooling the decocted liquid, and filtering by using a filter screen to obtain residue-free liquid A for later use;
b. putting 7 parts of vitamin additive, 5 parts of composite mineral, 0.6 part of amino acid, 0.8 part of calcium hydrophosphate, 0.3 part of ferric sulfate and 0.2 part of trace element into water with the temperature of 25 ℃ for mixing to obtain a mixed solution B;
c. mixing and crushing 6 parts of marigold root, 2 parts of pepper, 2 parts of saponin seed and 5 parts of mint leaf, drying at 55 ℃, and performing Soxhlet extraction to obtain a mixture C;
d. crushing 25 parts of wheat bran, 15 parts of sorghum, 2 parts of carrot, 6 parts of rice bran, 6 parts of fruit meal and 15 parts of corn, mixing with 1.5 parts of fish meal, 1.5 parts of raw eggs and 1.5 parts of fish oil, and uniformly stirring to obtain a mixture D for later use;
e. mixing and stirring the mixture C and the mixture D according to the weight ratio of 1:13, adding yeast fermentation liquor and bacillus fermentation liquor, uniformly stirring, carrying out sealed fermentation at 42 ℃ for 5 days, adding the dreg-free liquid A, uniformly stirring, and carrying out sealed fermentation at 57 ℃ for 22 days to obtain a fermented product;
f. mixing the mixed solution B with the fermented product according to the weight ratio of 2: 30, adding spirulina powder, sucrose powder, maltodextrin, flavoring agent and distilled water into the mixture, stirring for 25min at the rotating speed of 240r/min, drying and granulating to obtain the finished product.
Compared with the prior art, the invention has the following beneficial effects:
(1) according to the wormwood feed, a certain amount of wormwood is used as a raw material, the wormwood can stop diarrhea and relieve swelling, digestion and absorption of nutrient substances are enhanced, particularly, the immunity of the pig feed can be effectively improved, the digestion system of the pig feed can be improved, the infection of a live pig to African swine fever can be effectively prevented, other reasonably compatible traditional Chinese medicine components can greatly improve the resistance of the live pig, intestinal flora of the suckling pig can be adjusted, pathogenic escherichia coli, porcine transmissible gastroenteritis virus and other pathogenic bacteria causing diarrhea in intestinal tracts of the suckling pig can be killed in a non-irritant manner, accordingly, diarrhea of the suckling pig can be effectively prevented, other nutrient components have remarkable effects, the appetite of the pig can be improved, intestinal diseases can be prevented, the digestion is easy, and the weight can be increased reasonably.
(2) The wormwood feed provided by the invention is added with the vitamin additive, the compound mineral substances and the trace elements, so that on one hand, the nutritive value of the wormwood feed is improved, the nutrition of the wormwood feed is more comprehensive and balanced, on the other hand, the wormwood feed has good biocompatibility and gastrointestinal mucosa affinity, each loaded component has a controlled release effect, and the increment of pig feed pathogenic bacteria is effectively inhibited.
(3) The main nutrient substances in the feed are fermented, the fragrance is easy to emit, the food calling performance can be improved, excessive temperature is not generated in the preparation process, the vitamins and microbial agents contained in the feed can be prevented from being damaged due to high temperature, effective nutrients are guaranteed, the palatability is effectively improved, the digestibility is improved, the immunity is improved, the nutritional requirements of the growth and development of the live pigs are fully met, and the digestion function and the absorption function of the live pigs are effectively improved.
(4) The wormwood feed can effectively prevent live pigs from infecting African swine fever, is rich in nutrition and good in palatability, effectively improves the immunity of the live pigs, enhances the disease resistance, balances the nutrition, and has no chemical medicine residue; meanwhile, the preparation method has the advantages of low material cost, easily obtained raw materials, simple process, high practical value and good application prospect.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below with reference to specific embodiments, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1.
The preparation method of the wormwood feed for preventing the African swine fever comprises the following steps:
a. putting 15 parts of folium artemisiae argyi, 2 parts of radix isatidis, 2 parts of liquorice, 3 parts of radix astragali, 1 part of folium isatidis and 2 parts of herba violae into water, heating to 90 ℃, cooling the decocted liquid, and filtering by using a filter screen to obtain residue-free liquid A for later use;
b. putting 5 parts of vitamin additive, 4 parts of compound mineral, 0.5 part of amino acid, 0.7 part of calcium hydrophosphate, 0.2 part of ferric sulfate and 0.1 part of trace element into water with the temperature of 20 ℃ for mixing to obtain a mixed solution B;
c. mixing and crushing 5 parts of marigold root, 1 part of pepper, 1 part of saponin seed and 4 parts of mint leaf, drying at 50 ℃, and performing Soxhlet extraction to obtain a mixture C;
d. crushing 20 parts of wheat bran, 10 parts of sorghum, 1 part of carrot, 5 parts of rice bran, 5 parts of fruit pulp and 10 parts of corn, mixing with 1 part of fish meal, 1 part of raw egg and 1 part of fish oil, and uniformly stirring to obtain a mixture D for later use;
e. mixing and stirring the mixture C and the mixture D according to the weight ratio of 1:10, adding yeast fermentation liquor and bacillus fermentation liquor, uniformly stirring, hermetically fermenting at 40 ℃ for 3 days, adding the dreg-free liquid A, uniformly stirring, and hermetically fermenting at 55 ℃ for 20 days to obtain a fermented product;
f. mixing the mixed solution B with the fermented product according to the weight ratio of 2: 25, adding the spirulina powder, the sucrose powder, the maltodextrin, the flavoring agent and the distilled water into the mixture, stirring for 20min at the rotating speed of 200r/min, drying and granulating to obtain a finished product.
The vitamin additive in the embodiment comprises 0.5 part of vitamin A, 61.5 parts of vitamin B, 1.3 parts of vitamin D and 0.8 part of vitamin K.
In the embodiment, the solvent of Soxhlet extraction is chloroform, the extraction temperature is 75 ℃, and the extraction time is 5 h.
In this example, the yeast fermentation broth in step b is obtained by fermenting and culturing yeast at 28 ℃ for 3 days.
In this example, the Bacillus fermentation broth of step b is obtained by fermenting and culturing Bacillus at 33 deg.C for 4 days.
In the present embodiment, the bacillus is any one or a mixture of bacillus subtilis, bacillus cereus and bacillus curvatus.
The amino acid in this embodiment is one or more of methionine, glutamic acid, lysine, arginine, glutamine and glycine.
In this embodiment, the composite mineral in step b is formed by mixing iron salt, copper salt, manganese salt, zinc salt, selenium salt, cobalt salt, iodine salt and magnesium salt.
The preparation method of the spirulina powder in the embodiment comprises the following steps:
cleaning fresh spirulina, cutting, pulverizing in a pulverizer, spray drying for 1 ~ 3h, sieving with 80 ~ 200 mesh sieve, and microwave sterilizing for 1 ~ 3min to obtain the spirulina powder.
In this example, the added amounts of spirulina powder, sucrose powder, maltodextrin and flavoring agent were 6%, 3%, 2% and 1% by weight of the mixture in this step, respectively.
Example 2.
The preparation method of the wormwood feed for preventing the African swine fever comprises the following steps:
a. putting 25 parts of folium artemisiae argyi, 4 parts of radix isatidis, 4 parts of liquorice, 5 parts of radix astragali, 3 parts of folium isatidis and 4 parts of herba violae into water, heating to 100 ℃, cooling the decocted liquid, and filtering by using a filter screen to obtain residue-free liquid A for later use;
b. putting 9 parts of vitamin additive, 6 parts of composite mineral, 0.7 part of amino acid, 0.9 part of calcium hydrophosphate, 0.4 part of ferric sulfate and 0.3 part of trace element into water with the temperature of 30 ℃ for mixing to obtain a mixed solution B;
c. mixing and crushing 7 parts of marigold root, 3 parts of pepper, 3 parts of saponin seed and 6 parts of mint leaf, drying at 60 ℃, and performing Soxhlet extraction to obtain a mixture C;
d. crushing 30 parts of wheat bran, 20 parts of sorghum, 3 parts of carrot, 7 parts of rice bran, 7 parts of fruit meal and 20 parts of corn, mixing with 2 parts of fish meal, 2 parts of raw eggs and 2 parts of fish oil, and uniformly stirring to obtain a mixture D for later use;
e. mixing and stirring the mixture C and the mixture D according to the weight ratio of 1:16, adding yeast fermentation liquor and bacillus fermentation liquor, uniformly stirring, carrying out sealed fermentation at 45 ℃ for 6 days, adding the dreg-free liquid A, uniformly stirring, and carrying out sealed fermentation at 60 ℃ for 25 days to obtain a fermented product;
f. mixing the mixed solution B with the fermented product according to the weight ratio of 2: 35, adding the spirulina powder, the sucrose powder, the maltodextrin, the flavoring agent and the distilled water into the mixture, stirring for 30min at the rotating speed of 220r/min, drying and granulating to obtain the finished product.
The vitamin additive in the embodiment comprises 0.9 part of vitamin A, 62.9 parts of vitamin B, 3.7 parts of vitamin D and 2.3 parts of vitamin K.
In the embodiment, the solvent of Soxhlet extraction is chloroform, the extraction temperature is 80 ℃, and the extraction time is 7 h.
In this example, the yeast fermentation broth in step b is obtained by fermenting and culturing yeast at 29 deg.C for 5 days.
In this example, the Bacillus fermentation broth of step b is obtained by fermenting and culturing Bacillus at 35 deg.C for 4-6 days.
In the present embodiment, the bacillus is any one or a mixture of bacillus subtilis, bacillus cereus and bacillus curvatus.
The amino acid in this embodiment is one or more of methionine, glutamic acid, lysine, arginine, glutamine and glycine.
In this embodiment, the composite mineral in step b is formed by mixing iron salt, copper salt, manganese salt, zinc salt, selenium salt, cobalt salt, iodine salt and magnesium salt.
The preparation method of the spirulina powder in the embodiment comprises the following steps:
cleaning fresh spirulina, cutting, pulverizing in a pulverizer, spray drying for 1 ~ 3h, sieving with 80 ~ 200 mesh sieve, and microwave sterilizing for 1 ~ 3min to obtain the spirulina powder.
In this example, the added amounts of spirulina powder, sucrose powder, maltodextrin and flavoring agent were 6%, 3%, 2% and 1% by weight of the mixture in this step, respectively.
Example 3.
The preparation method of the wormwood feed for preventing the African swine fever comprises the following steps:
a. putting 20 parts of folium artemisiae argyi, 3 parts of radix isatidis, 3 parts of liquorice, 4 parts of radix astragali, 2 parts of folium isatidis and 3 parts of herba violae into water, heating to 95 ℃, cooling the decocted liquid, and filtering by using a filter screen to obtain residue-free liquid A for later use;
b. putting 7 parts of vitamin additive, 5 parts of composite mineral, 0.6 part of amino acid, 0.8 part of calcium hydrophosphate, 0.3 part of ferric sulfate and 0.2 part of trace element into water with the temperature of 25 ℃ for mixing to obtain a mixed solution B;
c. mixing and crushing 6 parts of marigold root, 2 parts of pepper, 2 parts of saponin seed and 5 parts of mint leaf, drying at 55 ℃, and performing Soxhlet extraction to obtain a mixture C;
d. crushing 25 parts of wheat bran, 15 parts of sorghum, 2 parts of carrot, 6 parts of rice bran, 6 parts of fruit meal and 15 parts of corn, mixing with 1.5 parts of fish meal, 1.5 parts of raw eggs and 1.5 parts of fish oil, and uniformly stirring to obtain a mixture D for later use;
e. mixing and stirring the mixture C and the mixture D according to the weight ratio of 1:13, adding yeast fermentation liquor and bacillus fermentation liquor, uniformly stirring, carrying out sealed fermentation at 42 ℃ for 5 days, adding the dreg-free liquid A, uniformly stirring, and carrying out sealed fermentation at 57 ℃ for 22 days to obtain a fermented product;
f. mixing the mixed solution B with the fermented product according to the weight ratio of 2: 30, adding spirulina powder, sucrose powder, maltodextrin, flavoring agent and distilled water into the mixture, stirring for 25min at the rotating speed of 240r/min, drying and granulating to obtain the finished product.
The vitamin additive in the embodiment comprises 0.7 part of vitamin A, 62 parts of vitamin B, 2 parts of vitamin D and 1.5 parts of vitamin K.
In the embodiment, the solvent of Soxhlet extraction is chloroform, the extraction temperature is 78 ℃, and the extraction time is 6 h.
In this embodiment, the yeast fermentation broth in step b is obtained by fermenting and culturing yeast at 28-29 deg.C for 34 days.
In this example, the Bacillus fermentation broth of step b is obtained by fermenting and culturing Bacillus at 34 deg.C for 5 days.
In the present embodiment, the bacillus is any one or a mixture of bacillus subtilis, bacillus cereus and bacillus curvatus.
The amino acid in this embodiment is one or more of methionine, glutamic acid, lysine, arginine, glutamine and glycine.
In this embodiment, the composite mineral in step b is formed by mixing iron salt, copper salt, manganese salt, zinc salt, selenium salt, cobalt salt, iodine salt and magnesium salt.
The preparation method of the spirulina powder in the embodiment comprises the following steps:
cleaning fresh spirulina, cutting, pulverizing in a pulverizer, spray drying for 1 ~ 3h, sieving with 80 ~ 200 mesh sieve, and microwave sterilizing for 1 ~ 3min to obtain the spirulina powder.
In this example, the added amounts of spirulina powder, sucrose powder, maltodextrin and flavoring agent were 6%, 3%, 2% and 1% by weight of the mixture in this step, respectively.
The performance test was performed on the wormwood pig feed prepared in each example above:
the test method comprises the following steps: selecting 60 healthy growing pigs which are similar in variety, age and weight and are healthy and have no disease and do not show symptoms of African swine fever, cleaning 6 pigsties in an African swine fever county area, fully sterilizing, feeding the 6 fattening pigs in the 6 cleaned pigsties, respectively adding the feed of examples 1-3 into 1-3 of the pigsties, respectively taking 4-6 as a control group, feeding the feed on the market, and obtaining the following results within 60 days of the test period, wherein the 10 healthy growing pigs are in one group and 6 groups in total, and the 6 groups are in the African swine fever county area:
| average daily gain g | The disease incidence rate% | |
| Group 1 | 4356 | 27 |
| 2 groups of | 4523 | 30 |
| Group 3 | 4412 | 22 |
| Comparative example 4 group | 3258 | 89 |
| Comparative example 5 group | 3391 | 95 |
| Comparative example 6 group | 3726 | 96 |
The control experiment shows that the wormwood feed can effectively prevent live pigs from infecting African swine fever, is rich in nutrition and good in palatability, effectively improves the immunity of the live pigs, enhances the disease resistance, balances the nutrition and has no chemical medicine residue; meanwhile, the preparation method has the advantages of low material cost, easily obtained raw materials, simple process, high practical value and good application prospect.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (10)
1. The preparation method of the wormwood feed for preventing African swine fever is characterized by comprising the following steps:
a. putting 15-25 parts of folium artemisiae argyi, 2-4 parts of radix isatidis, 2-4 parts of liquorice, 3-5 parts of radix astragali, 1-3 parts of folium isatidis and 2-4 parts of herba violae into water, heating to 90-100 ℃, cooling the decocted liquid, and filtering by using a filter screen to obtain residue-free liquid A for later use;
b. 5-9 parts of vitamin additive, 4-6 parts of compound mineral, 0.5-0.7 part of amino acid, 0.7-0.9 part of calcium hydrophosphate, 0.2-0.4 part of ferric sulfate and 0.1-0.3 part of trace element are put into water with the temperature of 20-30 ℃ for mixing to obtain mixed solution B;
c. mixing and crushing 5-7 parts of marigold root, 1-3 parts of pepper, 1-3 parts of saponin seeds and 4-6 parts of mint leaf, drying at 50-60 ℃, and performing Soxhlet extraction to obtain a mixture C;
d. crushing 20-30 parts of wheat bran, 10-20 parts of sorghum, 1-3 parts of carrots, 5-7 parts of rice bran, 5-7 parts of fruit meal and 10-20 parts of corns, mixing with 1-2 parts of fish meal, 1-2 parts of raw eggs and 1-2 parts of fish oil, and uniformly stirring to obtain a mixture D for later use;
e. mixing and stirring the mixture C and the mixture D according to the weight ratio of 1:10-16, adding yeast fermentation liquor and bacillus fermentation liquor, uniformly stirring, carrying out sealed fermentation at 40-45 ℃ for 3-6 days, adding the residue-free liquid juice A, uniformly stirring, and carrying out sealed fermentation at 55-60 ℃ for 20-25 days to obtain a fermented product;
f. mixing the mixed solution B with the fermented product according to the weight ratio of 2: 25-35, adding the spirulina powder, the sucrose powder, the maltodextrin, the flavoring agent and the distilled water into the mixture, stirring for 20-30min at the rotating speed of 200 plus 220r/min, drying and granulating to obtain the finished product.
2. The method for preparing moxa feed for prevention of African swine fever according to claim 1, wherein the vitamin supplement comprises vitamin A0.5-0.9 parts, vitamin B61.5-2.9 parts, vitamin D1.3-3.7 parts, vitamin K0.8-2.3 parts.
3. The method for preparing the wormwood feed for preventing the African swine fever according to claim 1, wherein the solvent for Soxhlet extraction is chloroform, the extraction temperature is 75-80 ℃, and the extraction time is 5-7 h.
4. The method for preparing wormwood feed for preventing african swine fever according to claim 1, wherein the yeast fermentation broth of the step b is obtained by fermenting and culturing yeast at 28-29 ℃ for 3-5 days;
the bacillus fermentation liquor in the step b is obtained by fermenting and culturing bacillus at the temperature of 33-35 ℃ for 4-6 days.
5. The method for preparing moxa feed for prevention of African swine fever according to claim 4, wherein the Bacillus is any one or a mixture of Bacillus subtilis, Bacillus cereus and Bacillus curvatus.
6. The method for preparing the wormwood feed for preventing African swine fever according to claim 1, wherein the amino acid is one or more of methionine, glutamic acid, lysine, arginine, glutamine and glycine.
7. The method for preparing wormwood feed for preventing African swine fever according to claim 1, wherein the composite minerals of step b are mixed with iron salt, copper salt, manganese salt, zinc salt, selenium salt, cobalt salt, iodine salt and magnesium salt.
8. The method for preparing the wormwood feed for preventing the African swine fever according to claim 1, wherein the method for preparing the spirulina powder comprises the following steps:
cleaning fresh spirulina, cutting, pulverizing in a pulverizer, spray drying for 1 ~ 3h, sieving with 80 ~ 200 mesh sieve, and microwave sterilizing for 1 ~ 3min to obtain the spirulina powder.
9. The method for preparing moxa feed for prevention of African swine fever according to claim 1, wherein the spirulina powder, sucrose powder, maltodextrin, and flavor enhancer are added in amounts of 6%, 3%, 2%, and 1% by weight of the mixture in this step, respectively.
10. The preparation method of the wormwood feed for preventing African swine fever according to claim 1, comprising the steps of:
a. putting 20 parts of folium artemisiae argyi, 3 parts of radix isatidis, 3 parts of liquorice, 4 parts of radix astragali, 2 parts of folium isatidis and 3 parts of herba violae into water, heating to 95 ℃, cooling the decocted liquid, and filtering by using a filter screen to obtain residue-free liquid A for later use;
b. putting 7 parts of vitamin additive, 5 parts of composite mineral, 0.6 part of amino acid, 0.8 part of calcium hydrophosphate, 0.3 part of ferric sulfate and 0.2 part of trace element into water with the temperature of 25 ℃ for mixing to obtain a mixed solution B;
c. mixing and crushing 6 parts of marigold root, 2 parts of pepper, 2 parts of saponin seed and 5 parts of mint leaf, drying at 55 ℃, and performing Soxhlet extraction to obtain a mixture C;
d. crushing 25 parts of wheat bran, 15 parts of sorghum, 2 parts of carrot, 6 parts of rice bran, 6 parts of fruit meal and 15 parts of corn, mixing with 1.5 parts of fish meal, 1.5 parts of raw eggs and 1.5 parts of fish oil, and uniformly stirring to obtain a mixture D for later use;
e. mixing and stirring the mixture C and the mixture D according to the weight ratio of 1:13, adding yeast fermentation liquor and bacillus fermentation liquor, uniformly stirring, carrying out sealed fermentation at 42 ℃ for 5 days, adding the dreg-free liquid A, uniformly stirring, and carrying out sealed fermentation at 57 ℃ for 22 days to obtain a fermented product;
f. mixing the mixed solution B with the fermented product according to the weight ratio of 2: 30, adding spirulina powder, sucrose powder, maltodextrin, flavoring agent and distilled water into the mixture, stirring for 25min at the rotating speed of 240r/min, drying and granulating to obtain the finished product.
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