CN103948035A - Peony seed oil microcapsules as well as preparation method and application thereof - Google Patents
Peony seed oil microcapsules as well as preparation method and application thereof Download PDFInfo
- Publication number
- CN103948035A CN103948035A CN201410195444.5A CN201410195444A CN103948035A CN 103948035 A CN103948035 A CN 103948035A CN 201410195444 A CN201410195444 A CN 201410195444A CN 103948035 A CN103948035 A CN 103948035A
- Authority
- CN
- China
- Prior art keywords
- seed oil
- peony seed
- yeast
- microcapsules
- wall material
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 235000006484 Paeonia officinalis Nutrition 0.000 title claims abstract description 108
- 235000015112 vegetable and seed oil Nutrition 0.000 title claims abstract description 104
- 239000003094 microcapsule Substances 0.000 title claims abstract description 96
- 238000002360 preparation method Methods 0.000 title claims abstract description 17
- 244000170916 Paeonia officinalis Species 0.000 title 1
- 241000736199 Paeonia Species 0.000 claims abstract description 110
- 239000000463 material Substances 0.000 claims abstract description 58
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 53
- 238000000034 method Methods 0.000 claims abstract description 23
- 238000005354 coacervation Methods 0.000 claims abstract description 19
- 235000013305 food Nutrition 0.000 claims abstract description 15
- 230000036541 health Effects 0.000 claims abstract description 6
- 239000002537 cosmetic Substances 0.000 claims abstract description 4
- 238000004945 emulsification Methods 0.000 claims abstract description 4
- 244000269722 Thea sinensis Species 0.000 claims abstract description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 54
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims description 43
- 235000010413 sodium alginate Nutrition 0.000 claims description 43
- 239000000661 sodium alginate Substances 0.000 claims description 43
- 229940005550 sodium alginate Drugs 0.000 claims description 43
- 108010073771 Soybean Proteins Proteins 0.000 claims description 28
- 235000019710 soybean protein Nutrition 0.000 claims description 28
- 240000005001 Paeonia suffruticosa Species 0.000 claims description 26
- 235000003889 Paeonia suffruticosa Nutrition 0.000 claims description 26
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 22
- 208000035404 Autolysis Diseases 0.000 claims description 20
- 206010057248 Cell death Diseases 0.000 claims description 20
- 230000028043 self proteolysis Effects 0.000 claims description 20
- 238000003756 stirring Methods 0.000 claims description 20
- 235000018102 proteins Nutrition 0.000 claims description 19
- 108090000623 proteins and genes Proteins 0.000 claims description 19
- 102000004169 proteins and genes Human genes 0.000 claims description 19
- 239000000839 emulsion Substances 0.000 claims description 16
- 239000003995 emulsifying agent Substances 0.000 claims description 15
- 230000009849 deactivation Effects 0.000 claims description 14
- 238000001291 vacuum drying Methods 0.000 claims description 14
- 238000009777 vacuum freeze-drying Methods 0.000 claims description 14
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 12
- 238000012856 packing Methods 0.000 claims description 12
- 239000002253 acid Substances 0.000 claims description 11
- 210000005253 yeast cell Anatomy 0.000 claims description 11
- 239000003513 alkali Substances 0.000 claims description 9
- 238000001879 gelation Methods 0.000 claims description 9
- 239000000725 suspension Substances 0.000 claims description 9
- 239000007788 liquid Substances 0.000 claims description 8
- 239000000047 product Substances 0.000 claims description 8
- -1 sucrose ester Chemical class 0.000 claims description 8
- JLPULHDHAOZNQI-ZTIMHPMXSA-N 1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCC\C=C/C\C=C/CCCCC JLPULHDHAOZNQI-ZTIMHPMXSA-N 0.000 claims description 7
- 229940083466 soybean lecithin Drugs 0.000 claims description 7
- 102000004190 Enzymes Human genes 0.000 claims description 6
- 108090000790 Enzymes Proteins 0.000 claims description 6
- 229930006000 Sucrose Natural products 0.000 claims description 6
- 238000005119 centrifugation Methods 0.000 claims description 6
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 6
- 150000002148 esters Chemical class 0.000 claims description 6
- 235000011187 glycerol Nutrition 0.000 claims description 6
- 235000013336 milk Nutrition 0.000 claims description 6
- 239000008267 milk Substances 0.000 claims description 6
- 210000004080 milk Anatomy 0.000 claims description 6
- 239000005720 sucrose Substances 0.000 claims description 6
- IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 claims description 5
- 239000003242 anti bacterial agent Substances 0.000 claims description 5
- 229940088710 antibiotic agent Drugs 0.000 claims description 5
- 235000010445 lecithin Nutrition 0.000 claims description 5
- 239000000787 lecithin Substances 0.000 claims description 5
- 229940067606 lecithin Drugs 0.000 claims description 5
- 239000002699 waste material Substances 0.000 claims description 5
- 239000012153 distilled water Substances 0.000 claims description 3
- 230000001804 emulsifying effect Effects 0.000 claims description 3
- 229960005150 glycerol Drugs 0.000 claims description 3
- 241000196324 Embryophyta Species 0.000 claims description 2
- 238000002156 mixing Methods 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 8
- 239000000126 substance Substances 0.000 abstract description 6
- 239000002775 capsule Substances 0.000 abstract description 3
- 238000001035 drying Methods 0.000 abstract description 3
- 239000000843 powder Substances 0.000 abstract description 3
- 230000008569 process Effects 0.000 abstract description 3
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 239000002994 raw material Substances 0.000 abstract description 2
- 239000007787 solid Substances 0.000 abstract description 2
- 239000002904 solvent Substances 0.000 abstract description 2
- 235000013361 beverage Nutrition 0.000 abstract 1
- 238000005538 encapsulation Methods 0.000 abstract 1
- 235000016709 nutrition Nutrition 0.000 abstract 1
- 230000035764 nutrition Effects 0.000 abstract 1
- 239000011162 core material Substances 0.000 description 31
- 239000000243 solution Substances 0.000 description 26
- 238000005406 washing Methods 0.000 description 13
- 239000004519 grease Substances 0.000 description 10
- 238000001914 filtration Methods 0.000 description 8
- 238000004321 preservation Methods 0.000 description 7
- 238000006243 chemical reaction Methods 0.000 description 6
- 239000003795 chemical substances by application Substances 0.000 description 6
- 238000001816 cooling Methods 0.000 description 6
- 238000010790 dilution Methods 0.000 description 6
- 239000012895 dilution Substances 0.000 description 6
- 238000001556 precipitation Methods 0.000 description 6
- 239000008223 sterile water Substances 0.000 description 6
- 239000006228 supernatant Substances 0.000 description 6
- TUSDEZXZIZRFGC-UHFFFAOYSA-N 1-O-galloyl-3,6-(R)-HHDP-beta-D-glucose Natural products OC1C(O2)COC(=O)C3=CC(O)=C(O)C(O)=C3C3=C(O)C(O)=C(O)C=C3C(=O)OC1C(O)C2OC(=O)C1=CC(O)=C(O)C(O)=C1 TUSDEZXZIZRFGC-UHFFFAOYSA-N 0.000 description 5
- PHOQVHQSTUBQQK-SQOUGZDYSA-N D-glucono-1,5-lactone Chemical compound OC[C@H]1OC(=O)[C@H](O)[C@@H](O)[C@@H]1O PHOQVHQSTUBQQK-SQOUGZDYSA-N 0.000 description 5
- 239000001263 FEMA 3042 Substances 0.000 description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 5
- LRBQNJMCXXYXIU-PPKXGCFTSA-N Penta-digallate-beta-D-glucose Natural products OC1=C(O)C(O)=CC(C(=O)OC=2C(=C(O)C=C(C=2)C(=O)OC[C@@H]2[C@H]([C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)O2)OC(=O)C=2C=C(OC(=O)C=3C=C(O)C(O)=C(O)C=3)C(O)=C(O)C=2)O)=C1 LRBQNJMCXXYXIU-PPKXGCFTSA-N 0.000 description 5
- 238000009792 diffusion process Methods 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- 238000004108 freeze drying Methods 0.000 description 5
- 235000012209 glucono delta-lactone Nutrition 0.000 description 5
- 239000000182 glucono-delta-lactone Substances 0.000 description 5
- 229960003681 gluconolactone Drugs 0.000 description 5
- 239000008103 glucose Substances 0.000 description 5
- 230000010355 oscillation Effects 0.000 description 5
- 235000010987 pectin Nutrition 0.000 description 5
- 239000001814 pectin Substances 0.000 description 5
- 229920001277 pectin Polymers 0.000 description 5
- 238000007789 sealing Methods 0.000 description 5
- 235000015523 tannic acid Nutrition 0.000 description 5
- LRBQNJMCXXYXIU-NRMVVENXSA-N tannic acid Chemical compound OC1=C(O)C(O)=CC(C(=O)OC=2C(=C(O)C=C(C=2)C(=O)OC[C@@H]2[C@H]([C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)O2)OC(=O)C=2C=C(OC(=O)C=3C=C(O)C(O)=C(O)C=3)C(O)=C(O)C=2)O)=C1 LRBQNJMCXXYXIU-NRMVVENXSA-N 0.000 description 5
- 229940033123 tannic acid Drugs 0.000 description 5
- 229920002258 tannic acid Polymers 0.000 description 5
- 238000003828 vacuum filtration Methods 0.000 description 5
- 239000000446 fuel Substances 0.000 description 4
- 239000012535 impurity Substances 0.000 description 3
- 230000009965 odorless effect Effects 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 239000010908 plant waste Substances 0.000 description 3
- 239000011347 resin Substances 0.000 description 3
- 229920005989 resin Polymers 0.000 description 3
- 238000005507 spraying Methods 0.000 description 3
- UILPJVPSNHJFIK-UHFFFAOYSA-N Paeonol Chemical compound COC1=CC=C(C(C)=O)C(O)=C1 UILPJVPSNHJFIK-UHFFFAOYSA-N 0.000 description 2
- DTOSIQBPPRVQHS-PDBXOOCHSA-N alpha-linolenic acid Chemical compound CC\C=C/C\C=C/C\C=C/CCCCCCCC(O)=O DTOSIQBPPRVQHS-PDBXOOCHSA-N 0.000 description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 239000000194 fatty acid Substances 0.000 description 2
- 229960001031 glucose Drugs 0.000 description 2
- 235000001727 glucose Nutrition 0.000 description 2
- 239000008187 granular material Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000000050 nutritive effect Effects 0.000 description 2
- 239000003921 oil Substances 0.000 description 2
- 235000019198 oils Nutrition 0.000 description 2
- 229960000292 pectin Drugs 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 239000012460 protein solution Substances 0.000 description 2
- 238000013517 stratification Methods 0.000 description 2
- WRIDQFICGBMAFQ-UHFFFAOYSA-N (E)-8-Octadecenoic acid Natural products CCCCCCCCCC=CCCCCCCC(O)=O WRIDQFICGBMAFQ-UHFFFAOYSA-N 0.000 description 1
- LQJBNNIYVWPHFW-UHFFFAOYSA-N 20:1omega9c fatty acid Natural products CCCCCCCCCCC=CCCCCCCCC(O)=O LQJBNNIYVWPHFW-UHFFFAOYSA-N 0.000 description 1
- QSBYPNXLFMSGKH-UHFFFAOYSA-N 9-Heptadecensaeure Natural products CCCCCCCC=CCCCCCCCC(O)=O QSBYPNXLFMSGKH-UHFFFAOYSA-N 0.000 description 1
- 101710132601 Capsid protein Proteins 0.000 description 1
- OYHQOLUKZRVURQ-HZJYTTRNSA-N Linoleic acid Chemical compound CCCCC\C=C/C\C=C/CCCCCCCC(O)=O OYHQOLUKZRVURQ-HZJYTTRNSA-N 0.000 description 1
- 239000005913 Maltodextrin Substances 0.000 description 1
- 229920002774 Maltodextrin Polymers 0.000 description 1
- 239000005642 Oleic acid Substances 0.000 description 1
- ZQPPMHVWECSIRJ-UHFFFAOYSA-N Oleic acid Natural products CCCCCCCCC=CCCCCCCCC(O)=O ZQPPMHVWECSIRJ-UHFFFAOYSA-N 0.000 description 1
- 241000233805 Phoenix Species 0.000 description 1
- 241000218201 Ranunculaceae Species 0.000 description 1
- 235000010489 acacia gum Nutrition 0.000 description 1
- 239000001785 acacia senegal l. willd gum Substances 0.000 description 1
- 150000001299 aldehydes Chemical class 0.000 description 1
- 235000020661 alpha-linolenic acid Nutrition 0.000 description 1
- 235000015173 baked goods and baking mixes Nutrition 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-M hydroxide Chemical compound [OH-] XLYOFNOQVPJJNP-UHFFFAOYSA-M 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- YLTGFGDODHXMFB-UHFFFAOYSA-N isoacetovanillon Natural products COC1=CC=C(C(C)=O)C=C1O YLTGFGDODHXMFB-UHFFFAOYSA-N 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- QXJSBBXBKPUZAA-UHFFFAOYSA-N isooleic acid Natural products CCCCCCCC=CCCCCCCCCC(O)=O QXJSBBXBKPUZAA-UHFFFAOYSA-N 0.000 description 1
- 150000002576 ketones Chemical class 0.000 description 1
- 235000020778 linoleic acid Nutrition 0.000 description 1
- OYHQOLUKZRVURQ-IXWMQOLASA-N linoleic acid Natural products CCCCC\C=C/C\C=C\CCCCCCCC(O)=O OYHQOLUKZRVURQ-IXWMQOLASA-N 0.000 description 1
- 229960004488 linolenic acid Drugs 0.000 description 1
- 229940035034 maltodextrin Drugs 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 description 1
- MLIBGOFSXXWRIY-UHFFFAOYSA-N paeonol Natural products COC1=CC=C(O)C(C(C)=O)=C1 MLIBGOFSXXWRIY-UHFFFAOYSA-N 0.000 description 1
- 150000002978 peroxides Chemical class 0.000 description 1
- 239000002861 polymer material Substances 0.000 description 1
- 229930182490 saponin Natural products 0.000 description 1
- 150000007949 saponins Chemical class 0.000 description 1
- 235000017709 saponins Nutrition 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000009941 weaving Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/152—Milk preparations; Milk powder or milk powder preparations containing additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F5/00—Coffee; Coffee substitutes; Preparations thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
- A23P10/00—Shaping or working of foodstuffs characterised by the products
- A23P10/30—Encapsulation of particles, e.g. foodstuff additives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/11—Encapsulated compositions
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/10—General cosmetic use
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Mycology (AREA)
- Epidemiology (AREA)
- Birds (AREA)
- Nutrition Science (AREA)
- Dermatology (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Manufacturing Of Micro-Capsules (AREA)
- General Preparation And Processing Of Foods (AREA)
Abstract
The invention relates to peony seed oil microcapsule powder. The yeast peony seed oil microcapsules are prepared from peony seed oil serving as a raw material and a yeast wall material by using a yeast microcapsule method or are prepared by emulsification, encapsulation, solidifying and drying of a complex coacervation capsule method. The produced peony seed oil microcapsules can be applied to solid beverages such as milky tea and coffee, are capable of improving the instant solubility of products and intensifying nutrition of the products and can also be directly applied to health products, cosmetics and food. Chemical solvents are not added in a peony seed oil microcapsule preparation process, so that the peony seed oil microcapsules are green, environment-friendly and beneficial to the physical health of eaters; the relatively low temperature is always maintained in the whole process, so that the activity of functional components in the peony seed oil can be kept to the maximum degree.
Description
Technical field
The invention belongs to peony seed oil deep process technology field, be specifically related to a kind of peony seed oil microcapsules of making taking peony seed oil as Raw material processing and its preparation method and application.
Background technology
Tree peony (Paeonia suffruticosa Andr.) belongs to Ranunculaceae Paeonia, and machaka, has very high ornamental value and pharmaceutical value.For a long time, the exploitation of tree peony deep processed product but seem serious and lag behind, and production added value is very low, does not form industrial chain.
Peony seeds is the elite place of peony plant, and peony seeds is not only nutritious but also contain various bioactivators.Detect through the PONY of internal authority testing agency, the contained unrighted acid of peony seed oil is up to more than 92%, wherein alpha-linolenic acid accounts for 42%, linoleic acid accounts for 28%, oleic acid accounts for 22%, also, containing tens kinds of bioactivators such as paeonol, tree peony saponins, nutritive value is very abundant, is a kind of fabulous natural health care source.Unrighted acid in peony seed oil, very easily be subject to the impact of the physics such as ambient light photograph, air, temperature, metal ion and chemical factor and change, produce the chemical substances such as peroxide, hydroxide, aldehyde, ketone, low-molecular-weight fatty acid, trans-fatty acid, not only reduce nutritive value of food and digestive utilization ratio, and the material producing is harmful to health.
Microcapsules technology refers to natural or synthesized polymer material, by even their the mixture embedding of solid, liquid, gas, to form the technology of pellicle or closing membrane.The material that inside is wrapped by is called core, and outer membrane is called wall material, and the microcapsule granule particle diameter that embedding forms is 1～1000 μ m.Owing to thering is state, quality, volume and the performance that can change material; protection sensitive composition; strengthen stability; control Release of core material; reduce or cover disagreeable taste and reduce volatility; the functions such as isolation component, microcapsules technology is widely used at aspects such as food, medicine, weaving, coating, agricultural, cosmetics industries.
Although peony seed oil also has the product (CN103609751A) that is prepared into microcapsules, but they adopt maltodextrin and Arabic gum as wall material, cost is high, mobility and compactness are all poor, therefore, be necessary to seek other preparation method microcapsules preparation cost is declined, the performances such as microcapsule product quality stability, embedding rate, mobility, compactness and the Bc of preparation all show good technical scheme.
Summary of the invention
The technical problem that the present invention solves is to provide a kind of method of utilizing New Wall Material to prepare peony seed oil microcapsules, peony seed oil microcapsules prepared by described method and the purposes of peony seed oil microcapsules.
The invention provides a kind of preparation method of peony seed oil microcapsules, described method comprises taking peony seed oil as core, and one of following component is prepared peony seed oil microcapsules for wall material:
(1) yeast;
(2) soybean protein isolate and sodium alginate; Or
(3) tree peony cake cake protein and sodium alginate.
Further, described peony seed oil derives from the red tree peony of phoenix or Paeonia papaveracea.
Preferably, described wall material is yeast, and further, described yeast is the waste yeast liquid of wine brewing or antibiotics plant, or food grade dry ferment.
Preferably, described method comprises the steps (1) yeast purifying or deactivation, (2) yeast autolysis, (3) packing.
Preferably, in described packing step, core: wall material mass ratio is 1: 2～3: 1.
Preferably, described yeast purifying or inactivation step, for getting wine brewing or antibiotics plant waste yeast liquid, by the sterile water wash of 2～3 times of volumes, are sieved respectively 2 times with 80 orders, 100 object sieves, remove hop resin and other larger impurity, under 3000～5000r/min normal temperature, centrifugal 5～10min, abandons supernatant, then uses sterile water wash 1～2 time, to supernatant colorless and odorless, centrifugally obtain milky yeast milk, dry through vacuum drying or spraying, make dry ferment.The food grade dry ferment of living, 65～70 DEG C of deactivation 20～30min, or ultra violet lamp deactivation.
Preferably, described yeast autolysis step is the dry ferment of getting after purifying or deactivation, add the water of 4～10 times to make suspension (being yeast: water is 1: 4～1: 10), add again autolysis promoter ethanol 0.5～5%, and to adjust Initial pH be 5～7, changing temperature autolysis 12～48h within the scope of 40～60 DEG C, at 80～95 DEG C, enzyme 10～the 15min that goes out, after 3000～5000r/min centrifugation, 30～40min, it is for subsequent use that 30～50 DEG C of vacuum drying or freeze drying obtain yeast cell wall.
Preferably, described packing step is taking peony seed oil as core, yeast after treatment is wall material, add peony seed oil and yeast sealing in 1: 2～3: 1 ratios of core wall material mass ratio, put into 50～80 DEG C of water bath with thermostatic control oscillators, frequency of oscillation 80～160r/min, vibration 6～12h, peony seed oil can enter yeast cells inside by diffusion effect, form microcapsules, vacuum filtration reclaims the peony seed oil not wrapping into, use again 2～3 residual peony seed oils of yeast surface of absolute ethanol washing, 30～50 DEG C of vacuum drying 20～30min or freeze drying, obtain peony seed oil microcapsules.
Preferably, described wall material is soybean protein isolate and sodium alginate, and further, the mass ratio of described soybean protein isolate and sodium alginate is 2: 1～5: 1.
Preferably, described method comprises taking peony seed oil as core, and soybean protein isolate and sodium alginate are wall material, through following steps (1) emulsification, (2) packing, (3) solidify, dry preparation and obtaining.
Preferably, described core and wall material mass ratio are 1: 2～2: 1.
Preferably, described emulsifying step is soybean protein isolate and distilled water to mix for 1: 20 in mass ratio～1: 30, add 60～80 DEG C of stirring and dissolving of certain emulsifying agent, with alkali regulator solution pH value to 7～9, make solution and sodium alginate there is identical electric charge.
Preferably, described emulsifying agent is one or more of citric acid monoglyceride, sucrose ester, lecithin, soybean lecithin, glycerine monofatty ester, and emulsifying agent addition is 0.1～0.3% of microcapsules gross mass.
Preferably, described packing step is 40～80 DEG C of temperature, add a certain proportion of peony seed oil, homogeneous 5～the 10min of 3000～6000r/min high speed forms the emulsion of homogeneous, with acid for adjusting pH value to 3～4 (isoelectric point of soybean protein isolate is 4.1), make soybean protein isolate and sodium alginate with opposite charges, and stirring at low speed 20～25min, the multiple condensed phase that wall material generation complex coacervation produces is wrapped in peony seed oil surface, form microcapsule suspensions, be diluted with water and be placed in ice bath and continue to be stirred to below 10 DEG C, make the gelation of gained complex coacervation thing.
Preferably, described curing, drying steps, is to add curing agent, and stirring at low speed is solidified 1～2h, and stratification is collected the capsule that wets after filtering, washing, and obtains solid-state peony seed oil microcapsules through 30～50 DEG C of vacuum freeze dryings.
Preferably, described curing agent is one or more of glucono-δ-lactone, glucose, pectin, tannic acid, and the addition of curing agent is 0.8～2% of microcapsules gross mass.
Preferably, described wall material is tree peony cake cake protein and sodium alginate, and further, the mass ratio of described tree peony cake cake protein and sodium alginate is 2: 1～5: 1.
Preferably, described method comprises taking peony seed oil as core, and tree peony cake cake protein and sodium alginate are wall material, through following steps (1) emulsification, (2) packing, (3) solidify, dry preparation and obtaining.
Preferably, described core and wall material mass ratio are 1: 2～2: 1.
Preferably, described emulsifying step is tree peony cake cake protein and distilled water to mix for 1: 20 in mass ratio～1: 30, add 60～80 DEG C of stirring and dissolving of certain emulsifying agent, with alkali regulator solution pH value to 7～9, make solution and sodium alginate there is identical electric charge.
Preferably, described emulsifying agent is one or more of citric acid monoglyceride, sucrose ester, lecithin, soybean lecithin, glycerine monofatty ester, and emulsifying agent addition is 0.1～0.3% of microcapsules gross mass.
Preferably, described packing step is 40～80 DEG C of temperature, add a certain proportion of peony seed oil, homogeneous 5～the 10min of 3000～6000r/min high speed forms the emulsion of homogeneous, with acid for adjusting pH value to 3.2～4.2 (isoelectric point of tree peony cake cake protein is 4.5), make tree peony cake cake protein and sodium alginate with opposite charges, and stirring at low speed 20～25min, the multiple condensed phase that wall material generation complex coacervation produces is wrapped in peony seed oil surface, form microcapsule suspensions, be diluted with water and be placed in ice bath and continue to be stirred to below 10 DEG C, make the gelation of gained complex coacervation thing.
Preferably, described curing, drying steps, is to add curing agent, and stirring at low speed is solidified 1～2h, and stratification is collected the capsule that wets after filtering, washing, and obtains solid-state peony seed oil microcapsules through 30～50 DEG C of vacuum freeze dryings.
Preferably, described curing agent is one or more of glucono-δ-lactone, glucose, pectin, tannic acid, and the addition of curing agent is 0.8～2% of microcapsules gross mass.
The peony seed oil microcapsules that the present invention also provides a kind of above-mentioned either method to prepare.
The present invention also provides a kind of application of above-mentioned peony seed oil microcapsules, and described application comprises the application in the fields such as food, health products, cosmetics, milk tea, coffee.
Preferably, described food comprises milk powder, instant food and bakery product.
In peony seed oil microscapsule powder preparation process of the present invention, do not use chemical solvent, not only environmental protection, and be of value to the healthy of eater; Whole process maintains lower temperature all the time, can keep to greatest extent the activity of functional components in peony seed oil, adopt wall material cost lower, especially tree peony cake cake protein, it is the accessory substance after peony seeds oil expression, utilize its albumen as wall material, turn waste into wealth, greatly improved the comprehensive value of peony seeds.Adopt the peony seed oil microcapsule granule size after wall material packing of the present invention even, rounding, smooth surface, embedding rate is high, fuel load is large, volatility low (without the peony seed oil volatility of embedding up to 55.32%), and also dispersed and mobility is all good.Grease after microencapsulation has not only kept inherent characteristic and the function of grease, can make up again peony seed oil and be difficult for the deficiency of preserving, can also give its many new good characteristic: microencapsulation powdered oil is subject to the such environmental effects degree such as temperature less, storage-stable improves, and can extend the shelf life of grease; Product is beneficial to transport, stores, takes, for food industry processing provides convenience; After grease microcapsule, improve the dispersiveness of grease, its dissolubility, emulsion dispersion ability to water all obviously strengthens, be applied in food and can improve its structural state, flavor characteristics, can be applicable to always can not use grease or be difficult to use in the food of grease.Therefore the grease after microencapsulation has not only overcome the multiple disadvantage of conventional grease, has more into water instantly, and stability is high, is convenient to the advantages such as transport, production, preservation, has greatly widened the scope of application of grease.
Detailed description of the invention
Further illustrate the present invention below in conjunction with embodiment, should be appreciated that these examples can not serve as restriction of the present invention, without departing from the spirit and substance of the case in the present invention, the amendment of doing or replace and all belong to scope of the present invention.If do not specialize, the means in following embodiment are conventional means known in the art.
Embodiment 1 yeast wall material is prepared peony seed oil microcapsules
Step 1 waste yeast liquid purifying
Get wine brewing or antibiotics plant waste yeast liquid, by the sterile water wash of 2 times of volumes, sieve respectively 2 times with 80 orders, 100 object sieves, remove hop resin and other larger impurity, centrifugal 10min under 3000r/min normal temperature, abandon supernatant, use again sterile water wash 1～2 time, to supernatant colorless and odorless, centrifugally obtain milky yeast milk, dry through vacuum drying or spraying, make dry ferment particle.
Step 2 yeast autolysis is prepared wall material
Get the yeast after purifying, add the water of 4 times to make suspension, add again autolysis promoter 0.5% ethanol, and adjust Initial pH 6, changing temperature autolysis 48h within the scope of 40～60 DEG C, at 80 DEG C, enzyme 15min goes out, after 3000r/min centrifugation 40min, it is for subsequent use that 50 DEG C of vacuum drying or freeze drying obtain yeast cell wall.
The preparation of step 3 peony seed oil microcapsules
Taking peony seed oil as core, yeast after treatment is wall material, in core: 1: 2 ratio of wall material mass ratio adds peony seed oil and yeast sealing, put into 50 DEG C of water bath with thermostatic control oscillators, frequency of oscillation 80r/min, vibration 12h, peony seed oil can enter yeast cells inside by diffusion effect, form microcapsules, vacuum filtration reclaims the peony seed oil not wrapping into, use 2 residual peony seed oils of yeast surface of absolute ethanol washing, 30 DEG C of vacuum drying 30min or freeze drying, obtain peony seed oil microcapsules again.
Embodiment 2 yeast wall materials are prepared peony seed oil microcapsules
The deactivation of step 1 dry ferment
By 65 DEG C of deactivation 30min of food grade dry ferment, or ultra violet lamp deactivation.
Step 2 yeast autolysis is prepared wall material
Get the dry ferment of deactivation, add 6 times of water to make suspension, add again autolysis promoter 1% ethanol, and to adjust Initial pH be 5, changing temperature autolysis 24h within the scope of 40～60 DEG C, at 95 DEG C, enzyme 10min goes out, after 5000r/min centrifugation 30min, it is for subsequent use that 30 DEG C of vacuum drying or freeze drying obtain yeast cell wall.
The preparation of step 3 peony seed oil microcapsules
Taking peony seed oil as core, yeast after treatment is wall material, add peony seed oil and yeast sealing in 1: 1 ratio of core wall material mass ratio, put into 70 DEG C of water bath with thermostatic control oscillators, frequency of oscillation 160r/min, vibration 6h, peony seed oil can enter yeast cells inside by diffusion effect, form microcapsules, vacuum filtration reclaims the peony seed oil not wrapping into, use 3 residual peony seed oils of yeast surface of absolute ethanol washing, 50 DEG C of vacuum drying 20min or freeze drying, obtain peony seed oil microcapsules again.
Embodiment 3 yeast wall materials are prepared peony seed oil microcapsules
Step 1 waste yeast liquid purifying
Get wine brewing or antibiotics plant waste yeast liquid, by the sterile water wash of 3 times of volumes, sieve respectively 2 times with 80 orders, 100 object sieves, remove hop resin and other larger impurity, centrifugal 5min under 5000r/min normal temperature, abandon supernatant, use again sterile water wash 1～2 time, to supernatant colorless and odorless, centrifugally obtain milky yeast milk, dry through vacuum drying or spraying, make dry ferment particle.
Step 2 yeast autolysis is prepared wall material
Get the yeast after purifying, add 8 times of water to make suspension, add again autolysis promoter 3% ethanol, and to adjust Initial pH be 7, changing temperature autolysis 12h within the scope of 40～60 DEG C, at 85 DEG C, enzyme 12min goes out, after 4000r/min centrifugation 35min, it is for subsequent use that 30 DEG C of vacuum drying or freeze drying obtain yeast cell wall.
The preparation of step 3 peony seed oil microcapsules
Taking peony seed oil as core, yeast after treatment is wall material, add peony seed oil and yeast sealing in 2: 1 ratios of core wall material mass ratio, put into 60 DEG C of water bath with thermostatic control oscillators, frequency of oscillation 120r/min, vibration 10h, peony seed oil can enter yeast cells inside by diffusion effect, form microcapsules, vacuum filtration reclaims the peony seed oil not wrapping into, use 3 residual peony seed oils of yeast surface of absolute ethanol washing, 40 DEG C of vacuum drying 25min or freeze drying, obtain peony seed oil microcapsules again.
Embodiment 4 yeast wall materials are prepared peony seed oil microcapsules
The deactivation of step 1 dry ferment
By 70 DEG C of deactivation 20min of food grade dry ferment, or ultra violet lamp deactivation.
Step 2 yeast autolysis is prepared wall material
Get the dry ferment of deactivation, add 10 times of water to make suspension, add again autolysis promoter 5% ethanol, and to adjust Initial pH be 6, changing temperature autolysis 36h within the scope of 40～60 DEG C, at 95 DEG C, enzyme 10min goes out, after 3000r/min centrifugation 35min, it is for subsequent use that 50 DEG C of vacuum drying or freeze drying obtain yeast cell wall.
The preparation of step 3 peony seed oil microcapsules
Taking peony seed oil as core, yeast after treatment is wall material, add peony seed oil and yeast sealing in 3: 1 ratios of core wall material mass ratio, put into 80 DEG C of water bath with thermostatic control oscillators, frequency of oscillation 100r/min, vibration 8h, peony seed oil can enter yeast cells inside by diffusion effect, form microcapsules, vacuum filtration reclaims the peony seed oil not wrapping into, use 2 residual peony seed oils of yeast surface of absolute ethanol washing, 30 DEG C of vacuum drying 20min or freeze drying, obtain peony seed oil microcapsules again.
Embodiment 5 soybean protein isolates and sodium alginate are prepared peony seed oil microcapsules
Step 1, with soybean protein isolate and sodium alginate be wall material, mass ratio is 2: 1, adds respectively the water of 30 times, and soybean protein isolate and sodium alginate are configured to about 3.33% solution.
Step 2, in soybean protein isolate solution, add emulsifying agent citric acid monoglyceride, addition is 0.1% of microcapsules gross mass, and stirring and dissolving at 60 DEG C, by the pH value to 9 of alkali regulator solution.
Step 3, in above-mentioned solution, add peony seed oil core, core and wall material mass ratio are 1: 2, at 40 DEG C of temperature, obtain the emulsion of stable homogeneous with the speed high-speed stirred 10min of 6000r/min, and with acid for adjusting pH value to 3.5, add sodium alginate soln and stir to above-mentioned emulsion droplet again, water bath heat preservation reaction 20min, forms complex coacervation thing in system.The water dilution cooling of the amount of doubling, and be placed in ice bath and continue to be stirred to below 10 DEG C, make the gelation of gained complex coacervation thing.
Step 4, add the glucono-δ-lactone of microcapsules gross mass 0.8% to above-mentioned system, at 40 DEG C of water-baths, solidify 2h, filtration washing precipitation, 30 DEG C of vacuum freeze dryings obtain peony seed oil microcapsules.
Embodiment 6 soybean protein isolates and sodium alginate are prepared peony seed oil microcapsules
Step 1, with soybean protein isolate and sodium alginate be wall material, mass ratio is 3: 1, adds respectively the water of 25 times, and soybean protein isolate and sodium alginate are configured to 4% solution.
Step 2, in soybean protein isolate solution, add emulsifying agent sucrose ester, addition is 0.2% of microcapsules gross mass, and stirring and dissolving at 70 DEG C, by the pH value to 8 of alkali regulator solution.
Step 3, in above-mentioned solution, add peony seed oil core, core and wall material mass ratio are 1: 1, at 60 DEG C of temperature, obtain the emulsion of stable homogeneous with the speed high-speed stirred 8min of 4500r/min, and with acid for adjusting pH value to 4, add sodium alginate soln and stir to above-mentioned emulsion droplet again, water bath heat preservation reaction 25min, forms complex coacervation thing in system.Add the water dilution cooling of qdx, and be placed in ice bath and continue to be stirred to below 10 DEG C, make the gelation of gained complex coacervation thing.
Step 4, add the pectin of microcapsules gross mass 1% to above-mentioned system, at 60 DEG C of water-baths, solidify 2h, filtration washing precipitation, 50 DEG C of vacuum freeze dryings obtain peony seed oil microcapsules.
Embodiment 7 soybean protein isolates and sodium alginate are prepared peony seed oil microcapsules
Step 1, with soybean protein isolate and sodium alginate be wall material, mass ratio is 4: 1, adds respectively the water of 20 times, and soybean protein isolate and sodium alginate are configured to 5% solution.
Step 2, in soybean protein isolate solution, add emulsifier lecithin and soybean lecithin, addition is 0.3% of microcapsules gross mass, lecithin: soybean lecithin mass ratio is 1: 1, and stirring and dissolving at 80 DEG C, by the pH value to 7 of alkali regulator solution.
Step 3, in above-mentioned solution, add peony seed oil core, core and wall material mass ratio are 2: 1, at 70 DEG C of temperature, obtain the emulsion of stable homogeneous with the speed high-speed stirred 5min of 5000r/min, and with acid for adjusting pH value to 3, add sodium alginate soln and stir to above-mentioned emulsion droplet again, water bath heat preservation reaction 20min, forms complex coacervation thing in system.The water dilution cooling of the amount of doubling, and be placed in ice bath and continue to be stirred to below 10 DEG C, make the gelation of gained complex coacervation thing.
Step 4, add the glucose of microcapsules gross mass 2% to above-mentioned system, at 70 DEG C of water-baths, solidify 1h, filtration washing precipitation, 30 DEG C of vacuum freeze dryings obtain peony seed oil microcapsules.
Embodiment 8 soybean protein isolates and sodium alginate are prepared peony seed oil microcapsules
Step 1, with soybean protein isolate and sodium alginate be wall material, mass ratio is 5: 1, adds respectively the water of 25 times, and soybean protein isolate and sodium alginate are configured to 4% solution.
Step 2, in soybean protein isolate solution, add emulsifying agent citric acid monoglyceride and glycerine monofatty ester, addition is 0.15% of microcapsules gross mass, citric acid monoglyceride and glycerine monofatty ester mass ratio are 2: 1, and stirring and dissolving at 80 DEG C, by the pH value to 9 of alkali regulator solution.
Step 3, in above-mentioned solution, add peony seed oil core, core and wall material mass ratio are 1: 1, at 80 DEG C of temperature, obtain the emulsion of stable homogeneous with the speed high-speed stirred 10min of 3000r/min, and with acid for adjusting pH value to 3, add sodium alginate soln and stir to above-mentioned emulsion droplet again, water bath heat preservation reaction 25min, forms complex coacervation thing in system.The water dilution cooling of the amount of doubling, and be placed in ice bath and continue to be stirred to below 10 DEG C, make the gelation of gained complex coacervation thing.
Step 4, add glucose and the tannic acid of microcapsules gross mass 2% to above-mentioned system, glucose: tannic acid mass ratio is 1: 1, solidifies 1h at 80 DEG C of water-baths, filtration washing precipitation, and 40 DEG C of vacuum freeze dryings obtain peony seed oil microcapsules.
Embodiment 9 tree peony cake cake proteins and sodium alginate are prepared peony seed oil microcapsules
Step 1, with tree peony cake cake protein and sodium alginate be wall material, mass ratio is 2: 1, adds respectively the water of 30 times, and tree peony cake cake protein and sodium alginate are configured to about 3.33% solution.
Step 2, in tree peony cake cake protein solution, add emulsifying agent sucrose ester and soybean lecithin, addition is 0.3% of microcapsules gross mass, and sucrose ester and soybean lecithin ratio are 1: 1, and stirring and dissolving at 60 DEG C, by the pH value to 9 of alkali regulator solution.
Step 3, in above-mentioned solution, add peony seed oil core, core and wall material mass ratio are 1: 2, at 40 DEG C of temperature, obtain the emulsion of stable homogeneous with the speed high-speed stirred 10min of 3000r/min, and with acid for adjusting pH value to 3.2, add sodium alginate soln and stir to above-mentioned emulsion droplet again, water bath heat preservation reaction 20min, forms complex coacervation thing in system.The water dilution cooling of the amount of doubling, and be placed in ice bath and continue to be stirred to below 10 DEG C, make the gelation of gained complex coacervation thing.
Step 4, add the tannic acid of microcapsules gross mass 1.5% to above-mentioned system, at 40 DEG C of water-baths, solidify 2h, filtration washing precipitation, 30 DEG C of vacuum freeze dryings obtain peony seed oil microcapsules.
Embodiment 10 tree peony cake cake proteins and sodium alginate are prepared peony seed oil microcapsules
Step 1, with tree peony cake cake protein and sodium alginate be wall material, mass ratio is 5: 1, adds respectively the water of 20 times, and tree peony cake cake protein and sodium alginate are configured to 5% solution.
Step 2, in tree peony cake cake protein solution, add emulsifying agent glycerine monofatty ester, addition is 0.1% of microcapsules gross mass, and stirring and dissolving at 80 DEG C, by the pH value to 7 of alkali regulator solution.
Step 3, in above-mentioned solution, add peony seed oil core, core and wall material mass ratio are 2: 1, at 80 DEG C of temperature, obtain the emulsion of stable homogeneous with the speed high-speed stirred 5min of 6000r/min, and with acid for adjusting pH value to 4.2, add sodium alginate soln and stir to above-mentioned emulsion droplet again, water bath heat preservation reaction 25min, forms complex coacervation thing in system.Add the water dilution cooling of qdx, and be placed in ice bath and continue to be stirred to below 10 DEG C, make the gelation of gained complex coacervation thing.
Step 4, add glucono-δ-lactone and the pectin of microcapsules gross mass 2% to above-mentioned system, glucono-δ-lactone and pectin mass ratio are 1: 2, at 80 DEG C of water-baths, solidify 1h, filtration washing precipitation, 50 DEG C of vacuum freeze dryings obtain peony seed oil microcapsules.
The peony seed oil microcapsule product making in order to evaluate the present invention, we,, from microencapsulation embedding rate, microcapsules fuel load, moisture content, outward appearance, dissolubility and the several aspects of dispersiveness, mobility and volatility, have carried out overall merit to microcapsule product.
Index explanation:
1, microcapsule embedded rate computing formula: peony seed oil core quality × 100% of peony seed oil quality in the microcapsules of embedding rate=make/add.
2, microcapsules fuel load computing formula: microcapsules quality × 100% of peony seed oil quality in the microcapsules of fuel load=make/make.
3, microcapsules moisture content calculates formula: microcapsules quality × 100% of moisture content=(the microcapsules quality after the microcapsules quality-oven dry taking)/take.
4, microcapsules volatility computing formula: microcapsules quality × 100% of volatility=(the microcapsules quality of microcapsules quality-100 that take DEG C effect after 20 minutes)/take.
Claims (10)
1. a preparation method for peony seed oil microcapsules, is characterized in that, described method comprises taking peony seed oil as core, and one of following component is prepared peony seed oil microcapsules for wall material:
(1) yeast;
(2) soybean protein isolate and sodium alginate; Or
(3) tree peony cake cake protein and sodium alginate.
2. the method for claim 1, is characterized in that, described wall material is yeast, preferred, and described yeast is the waste yeast liquid of wine brewing or antibiotics plant, or food grade dry ferment.
3. method as claimed in claim 2, is characterized in that, described method comprises the steps (1) yeast purifying or deactivation, (2) yeast autolysis, (3) packing.Preferably, in described packing step, core: wall material mass ratio is 1: 2～3: 1.
4. method as claimed in claim 3, it is characterized in that, described yeast autolysis step is the dry ferment of getting after purifying or deactivation, add the water of 4～10 times to make suspension (being yeast: water is 1: 4～1: 10), add again autolysis promoter ethanol 0.5～5%, and to adjust Initial pH be 5～7, changing temperature autolysis 12～48h within the scope of 40～60 DEG C, at 80～95 DEG C, enzyme 10～15min goes out, after 3000～5000r/min centrifugation, 30～40min, it is for subsequent use that 30～50 DEG C of vacuum drying or freeze drying obtain yeast cell wall.
5. the method for claim 1, is characterized in that, described wall material is soybean protein isolate and sodium alginate or tree peony cake cake protein and sodium alginate.Preferably, described core and wall material mass ratio are 1: 2～2: 1.Further preferred, in described wall material (soybean protein isolate or tree peony cake cake protein): the mass ratio of sodium alginate is 2: 1～5: 1.
6. method as claimed in claim 5, is characterized in that, described method comprises the steps that (1) emulsification, (2) packing, (3) are solidified, dry preparation and obtaining.
7. method as claimed in claim 6, it is characterized in that, described emulsifying step is soybean protein isolate or tree peony cake cake protein and distilled water mixing in 1: 20 in mass ratio～1: 30, add 60～80 DEG C of stirring and dissolving of certain emulsifying agent, with alkali regulator solution pH value to 7～9, make solution and sodium alginate there is identical electric charge.Preferably, described emulsifying agent is one or more of citric acid monoglyceride, sucrose ester, lecithin, soybean lecithin, glycerine monofatty ester, and emulsifying agent addition is 0.1～0.3% of microcapsules gross mass.
8. the method as described in claim 6 or 7, it is characterized in that, described packing step is 40～80 DEG C of temperature, add a certain proportion of peony seed oil, homogeneous 5～the 10min of 3000～6000r/min high speed forms the emulsion of homogeneous, with acid for adjusting pH value to 3～4 or, pH value to 3.2～4.2 make soybean protein isolate or tree peony cake cake protein and sodium alginate with opposite charges, and stirring at low speed 20～25min, the multiple condensed phase that wall material generation complex coacervation produces is wrapped in peony seed oil surface, form microcapsule suspensions, be diluted with water and be placed in ice bath and continue to be stirred to below 10 DEG C, make the gelation of gained complex coacervation thing.
9. the peony seed oil microcapsules that prepared by claim 1-8 either method.
10. the application of peony seed oil microcapsules claimed in claim 9, described application comprises the application in the fields such as food, health products, cosmetics, milk tea, coffee.
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