CN103071148B - Compound thymosin Alpha 1 injection for improving livability of piglet and preparation method - Google Patents
Compound thymosin Alpha 1 injection for improving livability of piglet and preparation method Download PDFInfo
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Abstract
The invention provides a compound thymosin Alpha 1 injection for improving the livability of piglet and the preparation method thereof, wherein the compound thymosin Alpha 1 injection is prepared by the followings: 1 to 2 portions of thymosin Alpha 1, 3 to 5 portions of taurine, 5 to 10 portions of glutamine, 10 to 15 portions of vitamin C, 20 to 35 portions of radix sophorae flavescentis, 10 to 15 portions of Chinese pulsatilla root, 5 to 10 portions of atractylis ovata, and 3 to 5 portions of medicine terminalia fruit. The compound provided by the invention has high curing effect, is safe, reliable and nontoxic, and has no residuals, can obviously improve the immunity of piglet, and can effectively prevent immune suppressed diseases caused by swine fever, porcine circovirus and the like; toxin inside intestinal canal can be neutralized and damaged intestinal mucosa can be repaired, the colony structure inside the intestinal tract can be optimized, stress reaction caused by piglet weaning can be alleviated, and yellow and white scour of piglets, weaning stress diarrhea and diarrhea can be effectively prevented and cured. The medicine produced can be used for effectively increasing the survival rate of piglet, practical application shows that the survival rate of piglet can reach more than 98 percent after injection. In the invention, chitosan is used as flocculant for researching, developing and purifying the traditional Chinese medicine, the cost is low, safety and innocuity can be ensured, and the operation is simple.
Description
Technical field
The invention belongs to veterinary drug preparation field, be specifically related to a kind of compound recipe injection liquid of thymic peptide alpha 1 and preparation method that improves piglet survival rate.
Background technology
The growth stage of pig is divided into piglet, nursery pig and growing and fattening pigs, but the piglet stage attacked by all kinds of pathogen, be swinery the highest stage of mortality rate in life, account for 80% left and right of whole swinery mortality rate.Piglet not yet grows perfectly due to autoimmune system, and premunition is poor, therefore very easily infects the digestive system disease such as the immunosuppressive disease that causes as swine fever, porcine circovirus etc. and piglet Huang, Hakuri.The immunosuppressive disease that swine fever, porcine circovirus etc. cause is at present one of principal disease of harm China pig industry always, and grow in intensity, particularly also higher for piglet hazardness mortality rate larger, that cause, to pig industry, caused serious economic loss.After weaned piglet, by eating breast milk, be changed to the feedstuff of eating solid, intestinal mucosa thus very easily weares and teares, cause the Villus atrophy of intestinal mucosa, nutritional labeling must absorb by fine hair in small intestinal, so nutritional labeling can not well be digested and assimilated, and causes enteral nutrition fermentation of materials, upsets normal intestinal flora environment, usually cause serious escherichia coli, Salmonella infection, cause intractable diarrhea.Escherichia coli, Salmonella produce intestinal endotoxin in metabolic process, very easily further cause septicemia, the death of aggravation piglet.
At present, on veterinary clinic, for the medicine of preventing and treating immunosuppressive disease seldom, and effect is not satisfactory, is difficult to meet clinical needs.Aspect control diarrhoea, generally adopting antibiotic, sulfa drugs, due to long-term abuse of antibiotics, sulfa drugs, make a lot of bacterial strains produce drug resistance, dosage increases greatly like this, Liver and kidney toxicity strengthens, not only increase drug cost, but also increased antibiotic remains and the harm of toxic and side effects to human body.On veterinary clinic, in order to solve immunosuppressive disease and diarrhoea class disease, also there is the Chinese medicine preparation of employing to prevent and treat, but mostly be powder, abnormal smells from the patient is large, and dosage is large, is difficult to Piglet by Oral administration, exist bioavailability poor, cause medical material waste, the situation that therapeutic effect is undesirable.
Summary of the invention
The invention provides a kind of compound recipe injection liquid of thymic peptide alpha 1 and preparation method that improves piglet survival rate, can significantly improve pigling immunity, effectively prevent and treat the immunosuppressive disease that swine fever, porcine circovirus etc. cause; In energy and intestinal endotoxin and repair impaired intestinal mucosa, optimize bacterial flora structure in intestinal, alleviate stress of baby pigs caused ablaction reaction, the effectively generation of control yellow and white dysentery of piglet, ablactation stress diarrhoea and septicemia, by controlling the above two large class diseases of piglet, thereby reach the object that greatly improves piglet survival ratio.
For solving the problems of the technologies described above, the technical solution used in the present invention is: a kind of compound recipe injection liquid of thymic peptide alpha 1 that improves piglet survival rate, by the raw material of following weight parts, made:
Thymosin alpha 1 1-2 part, taurine 3-5 part, glutamine 5-10 part, vitamin C 10-15 part, Radix Sophorae Flavescentis 20-35 part, Radix Pulsatillae 10-15 part, Rhizoma Atractylodis Macrocephalae 5-10 part, Fructus Chebulae 3-5 part.
A compound recipe injection liquid of thymic peptide alpha 1 that improves piglet survival rate, is characterized in that:
1 part of Thymosin alpha 1,4 parts of taurines, 8 parts of glutamine, 12 parts of vitamin Cs, 25 parts of Radix Sophorae Flavescentiss, 12 parts of the Radix Pulsatillaes, 7 parts of the Rhizoma Atractylodis Macrocephalaes, 4 parts of Fructus Chebulaes.
A compound recipe injection liquid of thymic peptide alpha 1 that improves piglet survival rate, is undertaken by the method for being prepared as follows:
(1) taking by weight Radix Sophorae Flavescentis 20-35 part, Radix Pulsatillae 10-15 part, Rhizoma Atractylodis Macrocephalae 5-10 part, Fructus Chebulae 3-5 part is raw material;
(2) above-mentioned medical material is mixed, be processed into coarse powder;
(3) with the drinking water that meets state health standards, by the weight ratio of water and medicine, be 3:1, mix, in room temperature
Lower immersion 2 hours;
(4) after soaking, decoct twice.The weight that adds for the first time water is 5-8 times of former weighing medical material weight, decocts 1.5 hours; The weight that adds for the second time water is 4-6 times of former weighing medical material weight, decocts 1.0 hours, merges decocting liquid twice, filters, and gets filtrate, standby;
(5) filtrate in (4) merging is decocted, filter, filtrate is concentrated into while being 1:1 with crude drug ratio, be cooled to 40 ℃-50 ℃, adding chitosan amount is the 2%-4% of medicine liquid volume, stirs, and places 12 hours, standby;
(6) pour out (5) supernatant, add other active carbon of injection stage of 0.3%, be heated to 80 ℃, be 30 minutes heat time heating time, after adsorption bleaching, filters; Standby;
(7) in (6) filtrate, use 20%-40% sodium hydroxide solution adjust pH, be adjusted to 5.5-7.0, with No. 4 filter sticks, be filtered to clarification, standby;
(8) take Thymosin alpha 1 1-2 part, taurine 3-5 part, glutamine 5-10 part, vitamin C 10-15 part, add in water for injection 30-50 part, be heated to 60-70 ℃, stirring and dissolving, standby;
(9) (7), (8) reserve liquid are merged, with 20%-40% sodium hydroxide solution adjust pH, be adjusted to 6.0-7.0, with injecting water in every ml medicinal liquid containing 1 gram of crude drug in whole.With No. 4 filter sticks, be filtered to clarification;
(10) with filling and sealing machine by above-mentioned liquid medicine filling in ampoule bottle, adopt 121 ℃, 0.15Mpa flowing steam sterilization 30 minutes.
Advantage of the present invention and useful effect are:
This product antibiotic-free residual, have no side effect, can significantly improve pigling immunity, in energy and intestinal endotoxin and repair impaired intestinal mucosa, optimize bacterial flora structure in intestinal, alleviate stress of baby pigs caused ablaction reaction.
2. this product is effectively treated immunosuppressive disease and yellow and white dysentery of piglet, ablactation stress diarrhoea and the septicemia that swine fever, porcine circovirus etc. cause.
3. for piglet birth every injection this product 0.2ml after 3 days, can effectively prevent the piglet must not HUANGBAI(sic) dysentery; At wean every injection this product 0.5ml on the same day, can effectively prevent stress of baby pigs caused ablaction diarrhoea.After above double injection, can improve the premunition of piglet, effectively prevent the various immunosuppressive disease of piglet, greatly improve piglet survival rate.
4. aspect Purification of traditional Chinese herbs, the present invention uses new technology research and development purification Chinese medicine.Chinese medicine extracts and adopts decoction and alcohol sedimentation technique as going deimpurity separation means, and decoction and alcohol sedimentation technique is not only wasted ethanol resource, and contaminated environment increase cost, and loss of effective components is serious, and finished product stability is poor.The present invention uses chitosan as flocculating agent, and chitosan not only has good flocculation, clarification, with low cost, safety non-toxic, easy and simple to handle, the extraction that can be used for most of Chinese herbal medicine is refining, and can improve body immunity, nontoxic, tasteless, have no side effect.
The advantages such as its curative effect is high, instant effect, safe and reliable, antibiotic-free is residual, have no side effect, the present invention uses new technology research and development purification Chinese medicine.Use chitosan as flocculating agent, chitosan not only has good flocculation, clarification, with low cost, and safety non-toxic is easy and simple to handle, and the extraction that can be used for most of Chinese herbal medicine is refining, and can improve body immunity, nontoxic, tasteless, have no side effect.
All medicines by rational compatibility, improve the immunocompetence of piglet from multi-angle above, discharge and in and intestinal endotoxin and repair impaired intestinal mucosa, optimize bacterial flora structure in intestinal, alleviate stress of baby pigs caused ablaction reaction.Thereby various immunosuppressive disease and yellow and white dysentery of piglet, ablactation stress diarrhoea and the septicemia of effectively controlling piglet, reach the object that improves piglet survival rate.
The specific embodiment
Embodiment: a kind of compound recipe injection liquid of thymic peptide alpha 1 that improves piglet survival rate, by the raw material of following weight parts, made:
Thymosin alpha 1 1-2 part, taurine 3-5 part, glutamine 5-10 part, vitamin C 10-15 part, Radix Sophorae Flavescentis 20-35 part, Radix Pulsatillae 10-15 part, Rhizoma Atractylodis Macrocephalae 5-10 part, Fructus Chebulae 3-5 part.
By the method for being prepared as follows, undertaken:
(1) taking by weight Radix Sophorae Flavescentis 20-35 part, Radix Pulsatillae 10-15 part, Rhizoma Atractylodis Macrocephalae 5-10 part, Fructus Chebulae 3-5 part is raw material;
(2) above-mentioned medical material is mixed, be processed into coarse powder;
(3) with the drinking water that meets state health standards, by the weight ratio of water and medicine, be 3:1, mix, at room temperature soak 2 hours;
(4) after soaking, decoct twice.The weight that adds for the first time water is 5-8 times of former weighing medical material weight, decocts 1.5 hours; The weight that adds for the second time water is 4-6 times of former weighing medical material weight, decocts 1.0 hours, merges decocting liquid twice, filters, and gets filtrate, standby;
(5) (4) filtrate merge is decocted, filter, filtrate is concentrated into while being 1:1 with crude drug ratio, be cooled to 40 ℃-50 ℃, adding chitosan amount is the 2%-4% of medicine liquid volume, stirs, and places 12 hours, standby;
(6) pour out (5) supernatant, add other active carbon of injection stage of 0.3%, be heated to 80 ℃, be 30 minutes heat time heating time, after adsorption bleaching, filters; Standby;
(7) in (6) filtrate, use 20%-40% sodium hydroxide solution adjust pH, be adjusted to 5.5-7.0, with 4
Number filter stick is filtered to clarification, standby;
(8) take Thymosin alpha 1 1-2 part, taurine 3-5 part, glutamine 5-10 part, vitamin C 10-15 part, add in water for injection 30-50 part, be heated to 60-70 ℃, stirring and dissolving, standby;
(9) (7), (8) reserve liquid are merged, with 20%-40% sodium hydroxide solution adjust pH, be adjusted to 6.0-7.0, with injecting water in every ml medicinal liquid containing 1 gram of crude drug in whole.With No. 4 filter sticks, be filtered to clarification;
(10) with filling and sealing machine by above-mentioned liquid medicine filling in ampoule bottle, adopt 121 ℃, 0.15Mpa flowing steam sterilization 30 minutes.
One, the acute toxicity test of the veterinary drug preparation of preparation to rat
1, materials and methods
1.1 animals: rat Wister Xi， Hebei North institute animal center provides body weight 180-200g.
1.2 methods: get 50 of rat, be divided at random 5 groups, 10 every group, male and female half and half are raised one week before experiment under controlled condition, are in a good state of health.This veterinary drug preparation is by the grouping of different dosing dosage, and gavage is for oral administration once a day, Continuous Observation 7 days.
Result: after rat this veterinary drug preparation for oral administration, the viewing duration at 7 days, drinking-water and feed intake are all normal, do not occur any clinical poisoning symptom and death.
The acute toxicity test of table 2 veterinary drug preparation to rat
Conclusion: oral formulation dosage of rat reaches 16(ml/kg), clinical recommendering folder preparation consumption is 0.1ml/kg, exceeded 100 times of clinical recommendation consumptions and still do not occurred above any poisoning symptom and death, according to toxicological evaluation standard, thought that this preparation belongs to innocuous substance.
Two, compound recipe injection liquid of thymic peptide alpha 1 of the present invention is as follows to the Curative effect of control yellow and white dysentery of piglet, ablactation stress diarrhoea and survival rate:
1 materials and methods
1.1 test drug
Compound recipe injection liquid of thymic peptide alpha 1 10ml*10 props up
Ceftiofur Hydrochloride injecta 10ml*10 props up content 10ml: 0.5g lot number: 20120302, and it is qualified that Henan animal pharmaceutical estate company produces the detection of ，Jing our company.
1.2 experimental animals and grouping
Animal subject, 300 of the healthy suckling pigs of nest three way cross 1 age in days, are divided into test group, positive controls and negative control group at random; Every group 100.
1.3 test method
On 3 ages in days of suckling pig and wean same day, to test group, positive controls and negative control group difference intramuscular injection compound recipe injection liquid of thymic peptide alpha 1, Ceftiofur Hydrochloride injecta and normal saline, test piglet carries out routine according to pig farm management and raises respectively.Concrete dosage is as following table:
Table 1 test piglet grouping and medicinal health-care consumption
Results and analysis
The impact of compound recipe injection liquid of thymic peptide alpha 1 health care on piglet survival rate
As can be seen from Table 1, after 3 ages in days of suckling pig and wean intramuscular injection on same day compound recipe injection liquid of thymic peptide alpha 1, the survival rate of test group piglet reaches 97%, higher by 12% than positive controls, difference is (P<0.01) extremely significantly, higher by 24% than negative control group, difference is (P<0.01) extremely significantly.
The impact of compound recipe injection liquid of thymic peptide alpha 1 health care on yellow and white dysentery of piglet
As can be seen from Table 1, after 3 ages in days of suckling pig and wean intramuscular injection on same day compound recipe injection liquid of thymic peptide alpha 1, the HUANGBAI(sic) dysentery incidence rate of test group piglet is 2%, lower by 6% than positive controls, difference is (P<0.01) extremely significantly, lower by 14% than negative control group, difference is (P<0.01) extremely significantly.
The impact of compound recipe injection liquid of thymic peptide alpha 1 health care on stress of baby pigs caused ablaction diarrhoea
As can be seen from Table 1, after 3 ages in days of suckling pig and wean intramuscular injection on same day compound recipe injection liquid of thymic peptide alpha 1, the ablactation stress diarrhea rate of test group piglet is 1%, lower by 6% than positive controls, difference is (P<0.01) extremely significantly, lower by 14% than negative control group, difference is (P<0.01) extremely significantly.
Three, the clinical observation on the therapeutic effect of compound recipe injection liquid of thymic peptide alpha 1 to the mixed infection of swine fever and pig gyrate virus II type
1 incidence
The raiser of Guangdong Province raises pigs 103 altogether, is porker, body weight approximately 15-20 kg.Occurred a kind of take cough, breathe, generate heat as the disease of principal character.Idiopathy rises day, and in 5 d, dead is 25.Field is main once with the antimicrobial drug treatments such as sulfanilamide, penicillin, DeGrain.Through Epidemiological study, clinical symptoms and laboratory diagnosis, be finally diagnosed as the mixed infection of swine fever and pig gyrate virus II type.
2 clinical symptoms
Sick pig is listless, disorderly thick by hair, palor, and the visible bean shape black speck having, high heat is delaied, 41 ℃ of left and right of body temperature, loss of appetite, thirsty wish increases, and defecates stiff or in the pasty state.Cough, breathing is urged, eye conjunctiva flushing, the exhaustion that the pig that the course of disease is longer has is dead, and what have becomes cad pig.
3 cut open inspection changes
Each intestinal segment is congested, hemorrhage, mesenteric lymph node and inguinal lymph nodes enlargement, hemorrhage, and tangent plane is marble sample; Antiadoncus; Renomegaly, congestion, surface is ditch and returns shape, and color is khaki, has petechial hemorrhage; Bladder mucosa has more petechia; The edema that gastric mucosa has, fatty degeneration of liver and being with
Yellow, pulmonary congestion, edema, congestion.
4 laboratory diagnosiss
4.1 swine fever direct immunofluorescence antibody tests
Cut open the sick pig of inspection, get the tissues such as its tonsil, kidney, lymph node.After quick-freezing 1 min, carry out frozen section, thickness is 6 μ m, and section exhibition is affixed in clean glass slide; After acetone is fixed 15 min, with the rinsing of PBS buffer, natural air drying; By the trivalent fluorescent antibody (purchased from China Veterinary Drugs Supervisory Inst.) of swine fever, pig parvoviral, pig virus diarrhoea, drip in slice surface, at 37 ℃, act on after 0.5 h, light rinsing, natural drying; Sealing cover plate, microscopy.In the fluorescence microscope visual field, in visible tissue epithelial cell slurry, present yellow, green or yellow green, nucleus is not painted, and cell outline is clearly demarcated, is the swine fever virus infection positive.
4.2 porcine circovirus multiplex polymerase chain re-actions (PCR) detect
Adopt the porcine circovirus PCR detection kit at Ministry of Agriculture's Disease Diagnosis of Veterinary center.Get serum, after 55 ℃ of digestion such as protease, RNA enzyme are spent the night, extract viral template DNA, carry out multiplex PCR amplification, after PCR finishes, get 15 μ L product electrophoresis in the agarose gel of 10 g/L, observed result in ultraviolet transilluminator.Result shows, the circovirus type II positive.
Index of assessment of curative effect:
Clinical efficacy standard: the clinical symptom disappearance in three days of fully recovering, the character such as stool colour and spiritual appetite are normal; In effective three days, clinical symptoms obviously alleviates, and spiritual appetite takes a turn for the better; In invalid three days, clinical symptoms is for disappearing, and spiritual appetite is poor, and the state of an illness continues development and dead.
The clinical efficacy statistics of table 3 the present invention to the mixed infection of swine fever and pig gyrate virus II type
Result of the test shows: medicine of the present invention has very definite and significant clinical efficacy, to the mixed infection cure rate of swine fever and pig gyrate virus II type, is 95.1%, and effective percentage is 98%.
Three, the impact test of compound recipe injection liquid of thymic peptide alpha 1 on piglet immunological function
1.1 experimental animal grouping and test methods
Animal subject, large-scale pig farm, Zhejiang, 20 of the healthy suckling pigs of nest three way cross 1 age in days, are divided into test group and matched group, 10 every group at random.On 3 ages in days of suckling pig and wean same day, to test group and matched group, intramuscular injection compound recipe injection liquid of thymic peptide alpha 1 and normal saline are respectively 0.2ml/ head, 0.5ml/ head respectively.Test piglet carries out routine according to pig farm management and raises.The blood sampling in latter 12 days of weaning, separation of serum, measures serum IgG, hemolysin, and separated leukocyte, carries out rosette formation test simultaneously.
Table 4 compound recipe injection liquid of thymic peptide alpha 1 is to piglet immunological Function detection result
Results and analysis:
The index of three detections all shows that medicine of the present invention has significant potentiation to piglet immunological function, test group is compared ring formation rate, hemolysin O D value, three indexs of IgG content with matched group, P < 0.05, shows experimental group and matched group significant difference aspect raising pigling immunity.
The pharmacological effect brief introduction of each raw material is as follows:
Thymosin alpha 1: the bioactive polypeptides matter that has that is thymus secretion.Be a kind of immunomodulator, mainly contain the effect that strengthens immunologic function.Can improve the immunologic function of the immunosuppressant animal due to a variety of causes induction.Have and regulate T lymphocyte to grow, break up and ripe effect, repair impaired T lymphocyte simultaneously, in body immune system, play an important role.
Taurine: 1. a kind of active substance that regulates body normal physiological activity, there is the digestion of the lipid of adjusting and absorb, improve immunity of organisms, strengthen cell membrane oxidation resistance, the growth-promoting factors of bacillus bifidus in taurine or intestinal, optimize the functions such as the interior bacterial flora structure of intestinal, alleviate stress of baby pigs caused ablaction reaction; 2. preventing and treating iron deficiency anemia has positive effect, and it not only can promote the absorption of intestinal to ferrum, can improve the speed of growth and the feed conversion rate of piglet; 3. taurine can be calm, analgesia and antiinflammatory, strong liver choleretic effect, nephrotoxicity is had to protective effect.
Glutamine: 1. maintain structure and the function of gut barrier, glutamine is the essential nutrient substance of intestinal mucosa cellular metabolism, plays a very important role maintaining the integrity of intestinal mucosa epithelial structure.Under stress state, in intestinal mucosa epithelial cell, glutamine is exhausted very soon.When intestinal lacks glutamine, intestinal mucosa atrophy, fine hair is thinning, shortening even comes off, and crypts shoals, and intestinal mucosal permeability increases, and intestinal immunity is impaired.Clinical practice proves, provides glutamine all can effectively prevent intestinal mucosa atrophy, improves intestinal immunity, reduces intestinal bacteria and endotoxin.2. glutamine has important immunoregulation effect, and it is lymphocytic emiocytosis, propagation and function thereof
Remain necessary.
Vitamin C: the immunity that 1. improves body: participate in the synthetic of immunoglobulin, leukocyte contains abundant VC, and intraleukocytic VC sharply reduces when organism infection, and VC can strengthen chemotaxis and the deformability of neutrophilic granulocyte, improves sterilizing ability.2. improve the anti-stress ability of body: body is subject to abnormal stimulation, can cause sympathetic activation, adrenal medulla and 17-hydroxy-11-dehydrocorticosterone secretion increasing.The epinephrine that adrenal medulla is secreted and norepinephrine are to have tyrosine to be transformed, in the participation of this process need VC.3. in and bacterial endotoxin, heavy dose can be faced upward virus replication processed, and enhancing body resistance also has antihistamine, anti-Kallidin I, chronic disease simultaneously
Radix Sophorae Flavescentis: heat clearing and damp drying; Wind dispelling insecticide.Main damp-heat dysentery; Discharging fresh blood stool; Jaundice; Dysuria; Edema; Leukorrhagia; Pudendal pruritus; Scabies; Leprosy; Skin pruritus; Noxious dampness skin infection.
The Radix Pulsatillae: heat-clearing and toxic substances removing; Eliminating pathogenic heat from blood to cure dysentery; Removing dampness and killing parasites
The Rhizoma Atractylodis Macrocephalae: invigorating the spleen and benefiting QI, dampness diuretic, hidroschesis, antiabortive.For diarrhea due to hypofunction of the spleen, edema, spontaneous perspiration, frequent fetal movement.
Fructus Chebulae: the property of medicine: bitter, sour, puckery, flat, return lung, large intestine channel; Effect: astringing lung-QI and relieving cough, relieving diarrhea with astringents, is the common medicine of controlling chronic diarrhea chronic dysentery.
The present invention selects Thymosin alpha 1, taurine, glutamine, vitamin C, from Multi-angle omnibearing, improves body immunity, strengthens the resistance against diseases of piglet, reaches effective control piglet immunological inhibition disease, greatly improves the object of piglet survival rate.
Taurine, vitamin C all can improve the anti-stress ability of body, effectively prevent and treat stress of baby pigs caused ablaction reaction, thereby can greatly improve piglet survival rate.
Glutamine, vitamin C all can discharge, in and body endotoxin, the septicemia of effectively alleviating HUANGBAI(sic) dysentery and the ablactation stress diarrhoea of piglet and avoiding being caused by endotoxin, thus can greatly improve piglet survival rate.
Taurine, glutamine are repaired the impaired mucosa of intestinal, improve intestinal microbial population environment, effectively prevent and treat HUANGBAI(sic) dysentery and the ablactation stress diarrhoea of piglet, thereby can greatly improve piglet survival rate.
Radix Sophorae Flavescentis, the Radix Pulsatillae, the Rhizoma Atractylodis Macrocephalae, Fructus Chebulae, the diarrhoea causing because of a variety of causes from multi-angle control piglet respectively.
Use chitosan as flocculating agent, chitosan not only has good flocculation, clarification, with low cost, and safety non-toxic is easy and simple to handle, and the extraction that can be used for most of Chinese herbal medicine is refining, and can improve body immunity, nontoxic, tasteless, have no side effect.
Claims (3)
1. improve a compound recipe injection liquid of thymic peptide alpha 1 for piglet survival rate, it is characterized in that being made by the raw material of following weight parts:
Thymosin alpha 1 1-2 part, taurine 3-5 part, glutamine 5-10 part, vitamin C 10-15 part, Radix Sophorae Flavescentis 20-35 part, Radix Pulsatillae 10-15 part, Rhizoma Atractylodis Macrocephalae 5-10 part, Fructus Chebulae 3-5 part.
2. a kind of compound recipe injection liquid of thymic peptide alpha 1 that improves piglet survival rate according to claim 1, is characterized in that:
1 part of Thymosin alpha 1,4 parts of taurines, 8 parts of glutamine, 12 parts of vitamin Cs, 25 parts of Radix Sophorae Flavescentiss, 12 parts of the Radix Pulsatillaes, 7 parts of the Rhizoma Atractylodis Macrocephalaes, 4 parts of Fructus Chebulaes.
3. the preparation method of a compound recipe injection liquid of thymic peptide alpha 1 that improves piglet survival rate is as follows:
(1) taking by weight Radix Sophorae Flavescentis 20-35 part, Radix Pulsatillae 10-15 part, Rhizoma Atractylodis Macrocephalae 5-10 part, Fructus Chebulae 3-5 part is raw material;
(2) above-mentioned medical material is mixed, be processed into coarse powder;
(3) with the drinking water that meets state health standards, by the weight ratio of water and medicine, be 3:1, mix, at room temperature soak 2 hours;
(4) after soaking, decoct twice, the weight that adds for the first time water is 5-8 times of former weighing medical material weight, decocts 1.5 hours; The weight that adds for the second time water is 4-6 times of former weighing medical material weight, decocts 1.0 hours, merges decocting liquid twice, filters, and gets filtrate, standby;
(5) (4) filtrate merge is decocted, filter, filtrate is concentrated into while being 1:1 with crude drug ratio, be cooled to 40 ℃-50 ℃, adding chitosan amount is the 2%-4% of medicine liquid volume, stirs, and places 12 hours, standby;
(6) pour out (5) supernatant, add other active carbon of injection stage of 0.3%, be heated to 80 ℃, be 30 minutes heat time heating time, after adsorption bleaching, filters; Standby;
(7) in (6) filtrate, use 20%-40% sodium hydroxide solution adjust pH, be adjusted to 5.5-7.0, with No. 4 filter sticks, be filtered to clarification, standby;
(8) take Thymosin alpha 1 1-2 part, taurine 3-5 part, glutamine 5-10 part, vitamin C 10-15 part, add in water for injection 30-50 part, be heated to 60-70 ℃, stirring and dissolving, standby;
(9) (7), (8) reserve liquid are merged, with 20%-40% sodium hydroxide solution adjust pH, be adjusted to 6.0-7.0, with injecting water in every ml medicinal liquid, contain 1 gram of crude drug in whole, with No. 4 filter sticks, be filtered to clarification;
(10) with filling and sealing machine by above-mentioned liquid medicine filling in ampoule bottle, adopt 121 ℃, 0.15Mpa flowing steam sterilization 30 minutes.
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| CN1628796A (en) * | 2004-08-20 | 2005-06-22 | 康合堂 | Medicine for treating ulcerative colitis |
| CN1660406A (en) * | 2004-12-16 | 2005-08-31 | 广东省农业科学院农业生物技术研究所 | Short peptide type nutriend emulsion inside bowel and producing method |
| CN102716209A (en) * | 2012-07-18 | 2012-10-10 | 河南中亚神鹏动物药业有限公司 | Veterinary drug composition for preventing porcine diarrheal disease |
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2013
- 2013-01-17 CN CN201310015887.7A patent/CN103071148B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1628796A (en) * | 2004-08-20 | 2005-06-22 | 康合堂 | Medicine for treating ulcerative colitis |
| CN1660406A (en) * | 2004-12-16 | 2005-08-31 | 广东省农业科学院农业生物技术研究所 | Short peptide type nutriend emulsion inside bowel and producing method |
| CN102716209A (en) * | 2012-07-18 | 2012-10-10 | 河南中亚神鹏动物药业有限公司 | Veterinary drug composition for preventing porcine diarrheal disease |
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