CN115089697A - American ginseng glycopeptide and preparation method and application thereof - Google Patents
American ginseng glycopeptide and preparation method and application thereof Download PDFInfo
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Abstract
The invention relates to the technical field of American ginseng glycopeptide, and particularly discloses a preparation method of American ginseng glycopeptide, which comprises the following steps: grinding radix Panacis Quinquefolii in a grinder to 100 mesh, freezing at-5 deg.C for 10min, and recovering to room temperature; then the mixture is sent into 4-6 times of extraction treatment agent for ultrasonic dispersion for 20-30min, the ultrasonic power is 400-500W, and after the ultrasonic treatment, the mixture is washed and dried for standby. The American ginseng glycopeptide is ground and then frozen, the treatment temperature is-5 ℃, the treatment is 10min, meanwhile, an extraction treatment agent is adopted for synergistic extraction, the extraction efficiency of the American ginseng glycopeptide is improved, the chitosan treatment agent takes chitosan as a main agent, and the raw materials formed by the synergistic effect of the American ginseng glycopeptide through the combination of the rhizoma atractylodis macrocephalae extract can promote the synergistic effect of the American ginseng glycopeptide and improve the curative effect of the product on treating diabetes.
Description
Technical Field
The invention relates to the technical field of American ginseng glycopeptide, and particularly relates to American ginseng glycopeptide and a preparation method and application thereof.
Background
American ginseng is a perennial herb of Panax of Araliaceae. The root is fleshy, the shape of the root is oval and spindle-shaped, the surface of the outer skin is light yellow, the root is delicate and smooth, the growth is luxuriant, and the texture of the cross section is chrysanthemum-shaped; the stem is in a vertical cylindrical shape, is smooth and unhaired, green or dark purple green, and has different heights according to different ages of the ginseng; the leaves are generally palm-shaped compound leaves consisting of 5 small leaves, the small leaves are inverted oval or oval, the leaves are thin, irregular thick sawteeth are arranged on the edges, generally, the annual American ginseng plant has compound leaves of only 1 small leaf with 3 small leaves, the biennial American ginseng plant has compound leaves of 1 or 2 opposite 5 small leaves, and the biennial American ginseng plant has compound leaves of 3-5 annular 5 small leaves; the flower is pulled out of the flower stem from the center of the stem top, and an umbrella-shaped inflorescence is formed by a plurality of small flowers; the flowering period and the fruit mature period are respectively 7 months and 9 months.
The existing American ginseng glycopeptide has poor extraction efficiency, and the effect of compounding other raw materials is difficult to know, so that the curative effect of the American ginseng glycopeptide applied to the treatment of diabetes is low.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide an American ginseng glycopeptide, a preparation method and an application thereof, so as to solve the problems in the background art.
The technical scheme adopted by the invention for solving the technical problems is as follows:
the invention provides a preparation method of American ginseng glycopeptide, which comprises the following steps:
the method comprises the following steps: grinding radix Panacis Quinquefolii in a grinder to 100 mesh, freezing at-5 deg.C for 10min, and recovering to room temperature;
step two: then sending the mixture into 4-6 times of extraction treatment agent for ultrasonic dispersion for 20-30min, wherein the ultrasonic power is 400-500W, and after the ultrasonic treatment is finished, washing and drying the mixture for later use;
step three: placing the product obtained in the step two in 5-9 times of 90% ethanol solution for reflux extraction at 55-65 deg.C for 1-3h, removing alcohol, and filtering to obtain first extractive solution;
step four: putting the filter residue obtained in the third step into 9-11 times of water, boiling with slow fire for 2-3h, filtering to obtain a second filtrate, and mixing the first extract and the second filtrate to obtain the glycopeptide complex;
step five: and mixing the glycopeptide complex with a chitosan treatment agent according to the weight ratio of 10:1 to finally obtain the American ginseng glycopeptide.
Preferably, the preparation method of the extraction treatment agent comprises the following steps:
s01: the nano silicon dioxide is sent into 3-5 times of silane coupling agent solution, and then hydrochloric acid is added, and the mixture is stirred and mixed uniformly;
s02: adding 5-10 parts of sodium dodecyl sulfate and 2-5 parts of oxalic acid into 20-30 parts of deionized water, and stirring and mixing fully to obtain an additive;
s03: and stirring and mixing the additive and the S01 product according to the weight ratio of 1:6 to obtain the extraction treating agent.
Preferably, the silane coupling agent solution is a silane coupling agent KH560 and ethanol according to a weight ratio of 1: (7-9).
Preferably, the particle size of the nano silicon dioxide is 10-20 nm.
Preferably, hydrochloric acid is added to the S01 to adjust the pH to 5.0.
Preferably, the preparation method of the chitosan treatment agent comprises the following steps:
s11: adding chitosan into water with the weight of 3-6 times of that of the chitosan, then adding acetic acid with the weight of 10-15% of the total weight of the chitosan, and stirring for dissolving;
s12: adding the white atractylodes rhizome into 6-10 times of water, boiling with slow fire, and then filtering to obtain a white atractylodes rhizome preparation solution;
s13: adding sodium alginate solution 20-30% of the total weight of chitosan of S11 and methylcellulose 1-5%, stirring and mixing thoroughly, adding Atractylodis rhizoma concoction solution 1-5% of the total weight of chitosan, stirring and mixing thoroughly to obtain chitosan treatment agent.
Preferably, the slow fire is boiled for 2-3 h.
Preferably, the mass fraction of the sodium alginate solution is 10-15%.
The invention also provides an application of the preparation method of the American ginseng glycopeptide in the American ginseng glycopeptide.
Compared with the prior art, the invention has the following beneficial effects:
the American ginseng glycopeptide is ground and then frozen, the treatment temperature is-5 ℃, the treatment is 10min, meanwhile, an extraction treatment agent is adopted for synergistic extraction, the extraction efficiency of the American ginseng glycopeptide is improved, the chitosan treatment agent takes chitosan as a main agent, and the raw materials formed by the synergistic effect of the American ginseng glycopeptide through the combination of the rhizoma atractylodis macrocephalae extract can promote the synergistic effect of the American ginseng glycopeptide and improve the curative effect of the product on treating diabetes.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below with reference to specific embodiments, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The preparation method of the American ginseng glycopeptide of the embodiment comprises the following steps:
the method comprises the following steps: grinding radix Panacis Quinquefolii in a grinder to 100 mesh, freezing at-5 deg.C for 10min, and recovering to room temperature;
step two: then sending the mixture into 4-6 times of extraction treatment agent for ultrasonic dispersion for 20-30min, wherein the ultrasonic power is 400-500W, and after the ultrasonic treatment is finished, washing and drying the mixture for later use;
step three: placing the product obtained in the step two in 5-9 times of 90% ethanol solution for reflux extraction at 55-65 deg.C for 1-3h, removing alcohol, and filtering to obtain first extractive solution;
step four: putting the filter residue obtained in the third step into 9-11 times of water, boiling with slow fire for 2-3h, filtering to obtain a second filtrate, and mixing the first extract and the second filtrate to obtain the glycopeptide complex;
step five: and mixing the glycopeptide complex with a chitosan treatment agent according to the weight ratio of 10:1 to finally obtain the American ginseng glycopeptide.
The preparation method of the extraction treatment agent of the embodiment comprises the following steps:
s01: the nano silicon dioxide is sent into 3-5 times of silane coupling agent solution, and then hydrochloric acid is added, and the mixture is stirred and mixed uniformly;
s02: adding 5-10 parts of sodium dodecyl sulfate and 2-5 parts of oxalic acid into 20-30 parts of deionized water, and stirring and mixing fully to obtain an additive;
s03: and stirring and mixing the additive and the S01 product according to the weight ratio of 1:6 to obtain the extraction treating agent.
The silane coupling agent solution of the embodiment is a silane coupling agent KH560 and ethanol in a weight ratio of 1: (7-9).
The particle size of the nano-silica of this example is 10 to 20 nm.
Hydrochloric acid was added to S01 of this example to adjust the pH to 5.0.
The preparation method of the chitosan treatment agent of the embodiment comprises the following steps:
s11: adding chitosan into water with the weight of 3-6 times of that of the chitosan, then adding acetic acid with the weight of 10-15% of the total weight of the chitosan, and stirring for dissolving;
s12: adding the white atractylodes rhizome into 6-10 times of water, boiling with slow fire, and then filtering to obtain a white atractylodes rhizome blending liquid;
s13: adding sodium alginate solution 20-30% of the total weight of chitosan of S11 and methylcellulose 1-5%, stirring and mixing thoroughly, adding Atractylodis rhizoma concoction solution 1-5% of the total weight of chitosan, stirring and mixing thoroughly to obtain chitosan treatment agent.
In the embodiment, the water is boiled for 2-3h by slow fire.
The mass fraction of the sodium alginate solution in the embodiment is 10-15%.
The method for preparing the American ginseng glycopeptide is applied to the American ginseng glycopeptide.
Example 1.
The preparation method of the American ginseng glycopeptide of the embodiment comprises the following steps:
the method comprises the following steps: grinding radix Panacis Quinquefolii in a grinder to 100 mesh, freezing at-5 deg.C for 10min, and recovering to room temperature;
step two: then sending the mixture into 4 times of extraction treatment agent for ultrasonic dispersion for 20min, wherein the ultrasonic power is 400W, and after the ultrasonic treatment, washing and drying the mixture for later use;
step three: placing the product obtained in the step two in 5 times of 90% ethanol solution by mass percent for reflux extraction for 1h, wherein the extraction temperature is 55 ℃, removing alcohol after the extraction is finished, and filtering to obtain a first extracting solution;
step four: putting the filter residue obtained in the third step into 9 times of water, boiling with slow fire for 2 hours, filtering to obtain a second filtrate, and combining the first extract and the second filtrate to obtain the glycopeptide complex;
step five: and mixing the glycopeptide complex with a chitosan treatment agent according to the weight ratio of 10:1 to finally obtain the American ginseng glycopeptide.
The preparation method of the extraction treatment agent of the embodiment comprises the following steps:
s01: sending the nano silicon dioxide into 3 times of silane coupling agent solution, then adding hydrochloric acid, and stirring and mixing uniformly;
s02: adding 5 parts of sodium dodecyl sulfate and 2 parts of oxalic acid into 20 parts of deionized water, and stirring and mixing fully to obtain an additive;
s03: and stirring and mixing the additive and the S01 product according to the weight ratio of 1:6 to obtain the extraction treating agent.
The silane coupling agent solution of the present embodiment is silane coupling agent KH560, ethanol in a weight ratio of 1: 7, preparing the medicament.
The particle size of the nanosilica of this example was 10 nm.
Hydrochloric acid was added to S01 of this example to adjust the pH to 5.0.
The preparation method of the chitosan treatment agent of the embodiment comprises the following steps:
s11: adding chitosan into water with the weight of 3 times, then adding acetic acid with the weight of 10 percent of the total weight of the chitosan, and stirring for dissolving;
s12: adding the white atractylodes rhizome into 6 times of water, boiling with slow fire, and then filtering to obtain a white atractylodes rhizome preparation solution;
s13: adding sodium alginate solution 20% of the total weight of chitosan of S11 and methylcellulose 1%, stirring and mixing thoroughly, finally adding Atractylodis rhizoma concoction solution 1% of the total weight of chitosan, stirring and mixing thoroughly to obtain chitosan treatment agent.
The water in the embodiment is boiled for 2 hours by mild fire.
The mass fraction of the sodium alginate solution in this example was 10%.
The method for preparing the American ginseng glycopeptide is applied to the American ginseng glycopeptide.
Example 2.
The preparation method of the American ginseng glycopeptide of the embodiment comprises the following steps:
the method comprises the following steps: grinding radix Panacis Quinquefolii in a grinder to 100 mesh, freezing at-5 deg.C for 10min, and recovering to room temperature;
step two: then sending the mixture into 4-6 times of extraction treatment agent for ultrasonic dispersion for 30min, wherein the ultrasonic power is 500W, and washing and drying the mixture for later use after finishing ultrasonic dispersion;
step three: placing the product obtained in the step two in 9 times of 90% ethanol solution by mass percent for reflux extraction for 3 hours, wherein the extraction temperature is 65 ℃, removing alcohol after the extraction is finished, and filtering to obtain a first extracting solution;
step four: putting the filter residue obtained in the third step into water 11 times the amount of the filter residue, boiling the filter residue with slow fire for 3 hours, filtering the mixture to obtain a second filtrate, and combining the first extract and the second filtrate to obtain the glycopeptide complex;
step five: and mixing the glycopeptide complex with a chitosan treatment agent according to the weight ratio of 10:1 to finally obtain the American ginseng glycopeptide.
The preparation method of the extraction treatment agent of the embodiment comprises the following steps:
s01: sending the nano silicon dioxide into 5 times of silane coupling agent solution, then adding hydrochloric acid, and stirring and mixing uniformly;
s02: adding 10 parts of sodium dodecyl sulfate and 5 parts of oxalic acid into 30 parts of deionized water, and fully stirring and mixing to obtain an additive;
s03: and stirring and mixing the additive and the S01 product according to the weight ratio of 1:6 to obtain the extraction treating agent.
The silane coupling agent solution of the embodiment is a silane coupling agent KH560 and ethanol in a weight ratio of 1: 9, preparing the composition.
The particle size of the nanosilica of this example was 20 nm.
Hydrochloric acid was added to S01 of this example to adjust the pH to 5.0.
The preparation method of the chitosan treatment agent of the embodiment comprises the following steps:
s11: adding chitosan into 6 weight times of water, adding acetic acid accounting for 15 percent of the total weight of the chitosan, and stirring for dissolving;
s12: adding the white atractylodes rhizome into 10 times of water, boiling with slow fire, and then filtering to obtain a white atractylodes rhizome preparation solution;
s13: adding sodium alginate solution 30% of the total weight of chitosan of S11 and methylcellulose 5%, stirring and mixing thoroughly, finally adding Atractylodis rhizoma concoction solution 5% of the total weight of chitosan, stirring and mixing thoroughly to obtain the chitosan treatment agent.
The water in the embodiment is boiled for 3 hours by mild fire.
The mass fraction of the sodium alginate solution in this example was 15%.
The method for preparing the American ginseng glycopeptide is applied to the American ginseng glycopeptide.
Example 3.
The preparation method of the American ginseng glycopeptide of the embodiment comprises the following steps:
the method comprises the following steps: grinding radix Panacis Quinquefolii in a grinder to 100 mesh, freezing at-5 deg.C for 10min, and recovering to room temperature;
step two: then sending the mixture into 5 times of extraction treatment agent for ultrasonic dispersion for 25min, wherein the ultrasonic power is 450W, and after the ultrasonic treatment, washing and drying the mixture for later use;
step three: placing the product obtained in the step two in 7 times of 90% ethanol solution by mass for reflux extraction for 2 hours at the extraction temperature of 60 ℃, removing alcohol after extraction is finished, and filtering to obtain a first extracting solution;
step four: putting the filter residue obtained in the third step into 10 times of water, boiling with slow fire for 2.5h, filtering to obtain a second filtrate, and combining the first extract and the second filtrate to obtain the glycopeptide complex;
step five: and mixing the glycopeptide complex with a chitosan treatment agent according to the weight ratio of 10:1 to finally obtain the American ginseng glycopeptide.
The preparation method of the extraction treatment agent of the embodiment comprises the following steps:
s01: sending the nano silicon dioxide into 4 times of silane coupling agent solution, then adding hydrochloric acid, and stirring and mixing uniformly;
s02: adding 7.5 parts of sodium dodecyl sulfate and 3.5 parts of oxalic acid into 25 parts of deionized water, and stirring and mixing fully to obtain an additive;
s03: and stirring and mixing the additive and the S01 product according to the weight ratio of 1:6 to obtain the extraction treating agent.
The silane coupling agent solution of the embodiment is a silane coupling agent KH560 and ethanol in a weight ratio of 1: 8, preparing the medicament.
The particle size of the nanosilica of this example was 15 nm.
Hydrochloric acid was added to S01 of this example to adjust the pH to 5.0.
The preparation method of the chitosan treatment agent of the embodiment comprises the following steps:
s11: adding chitosan into 4.5 weight times of water, adding acetic acid accounting for 12.5 percent of the total weight of the chitosan, and stirring for dissolving;
s12: adding the white atractylodes rhizome into 8 times of water, boiling with slow fire, and then filtering to obtain a white atractylodes rhizome preparation solution;
s13: adding sodium alginate solution accounting for 25 percent of the total weight of the chitosan of S11 and methylcellulose accounting for 3 percent of the total weight of the chitosan, stirring and mixing fully, finally adding bighead atractylodes rhizome blending solution accounting for 3 percent of the total weight of the chitosan, stirring and mixing fully to obtain the chitosan treating agent.
The water in the embodiment is boiled for 2.5h by mild fire.
The mass fraction of the sodium alginate solution in this example was 12.5%.
The method for preparing the American ginseng glycopeptide is applied to the American ginseng glycopeptide.
Example 4.
The preparation method of the American ginseng glycopeptide of the embodiment comprises the following steps:
the method comprises the following steps: grinding radix Panacis Quinquefolii in a grinder to 100 mesh, freezing at-5 deg.C for 10min, and recovering to room temperature;
step two: then sending the mixture into 5 times of extraction treatment agent for ultrasonic dispersion for 22min, wherein the ultrasonic power is 420W, and after the ultrasonic treatment, washing and drying the mixture for later use;
step three: placing the product obtained in the step two in an ethanol solution with the mass fraction of 90% of 6 times, performing reflux extraction for 1.2 hours at the extraction temperature of 56 ℃, removing alcohol after the extraction is finished, and filtering to obtain a first extracting solution;
step four: putting the filter residue obtained in the third step into 10 times of water, boiling with slow fire for 2.2h, filtering to obtain a second filtrate, and combining the first extract and the second filtrate to obtain the glycopeptide complex;
step five: and mixing the glycopeptide complex with a chitosan treatment agent according to the weight ratio of 10:1 to finally obtain the American ginseng glycopeptide.
The preparation method of the extraction treatment agent of the embodiment comprises the following steps:
s01: sending the nano silicon dioxide into 4 times of silane coupling agent solution, then adding hydrochloric acid, and stirring and mixing uniformly;
s02: adding 6 parts of sodium dodecyl sulfate and 3 parts of oxalic acid into 22 parts of deionized water, and fully stirring and mixing to obtain an additive;
s03: and stirring and mixing the additive and the S01 product according to the weight ratio of 1:6 to obtain the extraction treating agent.
The silane coupling agent solution of the present embodiment is silane coupling agent KH560, ethanol in a weight ratio of 1: 8, preparing the medicament.
The particle size of the nanosilica of this example was 12 nm.
Hydrochloric acid was added to S01 of this example to adjust the pH to 5.0.
The preparation method of the chitosan treatment agent of the embodiment comprises the following steps:
s11: adding chitosan into water with the weight of 4 times, then adding acetic acid with the weight of 12 percent of the total weight of the chitosan, and stirring for dissolving;
s12: adding the white atractylodes rhizome into water 7 times the weight of the white atractylodes rhizome, boiling the white atractylodes rhizome with slow fire, and then filtering the mixture to obtain white atractylodes rhizome blending liquid;
s13: adding sodium alginate solution (22 wt% of chitosan S11) and methylcellulose (2 wt% of chitosan S11), stirring, mixing, adding Atractylodis rhizoma solution (2 wt% of chitosan), stirring, and mixing to obtain chitosan treating agent.
The water in the embodiment is boiled for 2.2 hours by mild fire.
The mass fraction of the sodium alginate solution in this example was 12%.
The preparation method of the American ginseng glycopeptide of the embodiment is applied to the American ginseng glycopeptide.
Comparative example 1.
Unlike example 3, no extraction treatment was used.
Comparative example 2.
Unlike example 3, no chitosan treatment was added.
Comparative example 3.
Different from the example 3, no bighead atractylodes rhizome preparation solution is added in the preparation of the chitosan treatment agent.
Comparative example 4.
Different from the embodiment 3, the chitosan treating agent is prepared by adopting chitosan and water according to the weight ratio of 1: 5.
Comparative example 5.
The difference from example 3 is that no additive was added in the preparation of the extraction treatment agent.
Comparative example 6.
Different from the embodiment 3, the preparation method of the extraction treating agent is different;
s01: the nano silicon dioxide is put into 4 times of water, then hydrochloric acid is added, and the mixture is stirred and mixed evenly;
s02: 6 parts of sodium lauryl sulfate was added to 10 parts of the product S01, and mixed with stirring to obtain an extraction treatment.
The concentration of rat islet beta cells was controlled to 2X 10 cells/ml, cultured for 3 days in RPMT-1640 medium, cultured with the products of examples 1-3 and comparative examples 1-6 at an amount of 60ug/ml, and the cell viability and insulin secretion were determined
The performance test was as follows:
as can be seen from comparative examples 1 to 6 and examples 1 to 3;
the cell survival rate and the insulin secretion amount in the embodiment 3 of the invention are optimal, and meanwhile, the comparison examples 1-6 show that the cell survival rate and the insulin secretion amount are remarkably reduced without adopting the extraction treatment agent and adding the chitosan treatment agent, the cell survival rate and the insulin secretion amount are both reduced due to different preparation methods of the extraction treatment agent and the chitosan treatment agent, the effect of treating diabetes by only adopting the method of the invention is optimal, and the extraction treatment agent and the chitosan treatment agent can be synergized to jointly enhance the diabetes treatment efficiency; the preparation of the chitosan treating agent is not added with the bighead atractylodes rhizome blending liquid, and the preparation of the chitosan treating agent is prepared by adopting chitosan and water according to the weight ratio of 1: 5; the efficiency of treating diabetes is poor when no additive is added in the preparation of the extraction treatment agent.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (9)
1. A preparation method of American ginseng glycopeptides is characterized by comprising the following steps:
the method comprises the following steps: grinding radix Panacis Quinquefolii in a grinder to 100 mesh, freezing at-5 deg.C for 10min, and recovering to room temperature;
step two: then sending the extract into an extraction treatment agent with 4-6 times of the extract for ultrasonic dispersion for 20-30min, wherein the ultrasonic power is 400-500W, and washing and drying the extract for later use after the ultrasonic treatment is finished;
step three: placing the product obtained in the step two in 5-9 times of 90% ethanol solution for reflux extraction at 55-65 deg.C for 1-3h, removing alcohol, and filtering to obtain first extractive solution;
step four: putting the filter residue obtained in the third step into 9-11 times of water, boiling with slow fire for 2-3h, filtering to obtain a second filtrate, and mixing the first extract and the second filtrate to obtain the glycopeptide complex;
step five: and mixing the glycopeptide complex with a chitosan treatment agent according to the weight ratio of 10:1 to finally obtain the American ginseng glycopeptide.
2. The method of claim 1, wherein the extraction treatment agent is prepared by:
s01: the nano silicon dioxide is sent into 3-5 times of silane coupling agent solution, and then hydrochloric acid is added, and the mixture is stirred and mixed uniformly;
s02: adding 5-10 parts of sodium dodecyl sulfate and 2-5 parts of oxalic acid into 20-30 parts of deionized water, and stirring and mixing fully to obtain an additive;
s03: and stirring and mixing the additive and the S01 product according to the weight ratio of 1:6 to obtain the extraction treating agent.
3. The method of claim 2, wherein the silane coupling agent solution is silane coupling agent KH560, ethanol in a weight ratio of 1: (7-9).
4. The method of claim 2, wherein the nanosilicon dioxide has a particle size of 10-20 nm.
5. The method of claim 2, wherein hydrochloric acid is added to the S01 to adjust the pH to 5.0.
6. The method of claim 1, wherein the chitosan treatment agent is prepared by:
s11: adding chitosan into 3-6 times of water by weight, adding acetic acid accounting for 10-15% of the total weight of the chitosan, and stirring for dissolving;
s12: adding the white atractylodes rhizome into 6-10 times of water, boiling with slow fire, and then filtering to obtain a white atractylodes rhizome preparation solution;
s13: adding sodium alginate solution 20-30% of the total weight of chitosan of S11 and methylcellulose 1-5%, stirring and mixing thoroughly, adding Atractylodis rhizoma concoction solution 1-5% of the total weight of chitosan, stirring and mixing thoroughly to obtain chitosan treatment agent.
7. The method of claim 6, wherein the American ginseng glycopeptide is boiled with slow fire for 2 to 3 hours.
8. The method of claim 6, wherein the sodium alginate solution comprises 10-15% by weight of the glycopeptide.
9. Use of a method of preparing an American ginseng glycopeptide as claimed in any one of claims 1 to 8 in an American ginseng glycopeptide.
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| CN116585255A (en) * | 2023-04-24 | 2023-08-15 | 广州领衔生物科技有限公司 | Composition containing lactobacillus fermentation product and preparation method and application thereof |
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