CN110420143B - A gel containing herba seu radix Clerodenri Cyrtophylli extract and its preparation method - Google Patents
A gel containing herba seu radix Clerodenri Cyrtophylli extract and its preparation method Download PDFInfo
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- CN110420143B CN110420143B CN201910803016.9A CN201910803016A CN110420143B CN 110420143 B CN110420143 B CN 110420143B CN 201910803016 A CN201910803016 A CN 201910803016A CN 110420143 B CN110420143 B CN 110420143B
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- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention belongs to the field of cosmetics and discloses a gel containing a nine-class nymphaea hybrid extract and a preparation method thereof. The gel mainly comprises the following components: glycerol, xanthan gum, water, carbomer, betaine, nicotinamide, arginine, a tremella extract, carboxymethyl chitosan, a dendrobium officinale stem extract, a jiupin nymphaea hybrid extract, a black tiger extract, 1, 3-butanediol, an essence, a solubilizer and a preservative. The water lily extract and the black tiger fruit extract are used in a compatible manner, the water lily extract is used as a monarch, the black tiger fruit extract is used as a minister, the effectiveness and the stability of the product can be obviously improved, the skin can fully absorb and utilize effective components in the formula, and epidermal cells can be protected from being invaded by free radicals after long-term use, so that the effects of improving skin quality, moistening the skin, whitening and moisturizing, resisting wrinkles and fading spots, calming and resisting allergy are achieved.
Description
Technical Field
The invention belongs to the field of cosmetics, and particularly relates to a gel containing a nine-class nymphaea hybrid extract and a preparation method thereof.
Background
Along with the increasing living standard of people, people have more and more requirements on beauty and higher requirements on functions of skin care products. The traditional skin care cosmetics mainly comprise nutritional ingredients and water retention ingredients to protect, clean and improve the skin softness. However, the traditional skin care product raw materials contain a large amount of chemical compositions, and the skin care product raw materials provide certain skin care effect and improve the appearance, and simultaneously bring some inevitable damage to the skin. Therefore, people are pursuing natural skin care products without toxicity and side effects to achieve the effects of delaying aging, eliminating wrinkles and recovering elasticity and softness.
The gel is generally like a semitransparent milky substance, has thicker texture than common lotion, is lighter than essence, has liquid gel texture, is fresh and easy to absorb, and does not cause any burden on skin. The gel has the functions of conditioning skin and supplying moisture to horny layer, and simultaneously contains plant essence and mineral spring factors, can be durably acted on the skin, improve water-deficient wrinkles and regulate the water-oil balance of the skin, and is a skin care product which is necessary in four seasons of spring, summer, autumn and winter. However, the existing condensation on the market has single function and limited effect.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention mainly aims to provide the gel containing the jiupin nymphaea hybrid extract;
the invention also aims to provide a preparation method of the gel containing the jiupin nymphaea hybrid extract.
The purpose of the invention is realized by the following scheme:
a gel containing a nine-class nymphaea hybrid extract comprises the following components in percentage by mass:
a1 component: 5% of glycerol and 0.05% of xanthan gum;
a2 component: 62.87% of water;
a3 component: 0.2% of carbomer;
the component B comprises: betaine 0.5%, nicotinamide 2%, water 5%;
and the component C is as follows: 0.1 percent of arginine;
and (D) component: 1% of tremella extract, 0.2% of carboxymethyl chitosan, 1% of dendrobium officinale stem extract, 1% of jiupin nymphaea hybrid extract and black tiger extract; wherein the sum of the quality percentage of the nine-class nymphaea hybrid extract and the black tiger extract is 10 percent;
e1 component: 0.02% of essence and 0.06% of solubilizer;
e2 component: 10% of water;
and F component: 1% of 1, 3-butanediol and 1% of preservative.
Preferably, in the gel containing the asphodeloides bunge extract, the mass percentage of the asphodeloides bunge extract is 7%, and the mass percentage of the black tiger extract is 3%;
preferably, in the gel containing the jipin nymphaea hybrid extract, the mass percentage of the jipin nymphaea hybrid extract is 5%, and the mass percentage of the black tiger extract is 5%;
preferably, in the gel containing the jipin nymphaea hybrid extract, the mass percentage of the jipin nymphaea hybrid extract is 3%, and the mass percentage of the black tiger extract is 7%;
the solubilizer is preferably 1, 3-butanediol; the preservative is preferably PHL (caprylhydroxamic acid &1,2 hexanediol &1,3 propylene glycol).
The extraction method of the tremella extract refers to Chinese patent publication 'CN 201610693617.5-a low molecular weight tremella polysaccharide and its preparation method and application'.
The extraction method of the dendrobium officinale stem extract refers to Chinese patent publication 'CN 201810743725.9-an ultra-low molecular weight dendrobium officinale polysaccharide and preparation and application thereof'.
The nine-class nymphaea hybrid extract is extracted by the following method:
(1) taking fresh nine-product nymphaea hybrid, cleaning, drying surface water, adding the surface water into a tissue triturator, homogenizing into a viscous liquid, adding water into the obtained homogenate liquid, stirring and extracting, then filtering, concentrating the obtained filtrate, adding ethanol with the mass concentration of 95%, fully mixing, filtering after precipitation, and collecting precipitate;
(2) dissolving the obtained precipitate with hot water at 50-80 ℃, adding activated carbon for decolorization, then filtering to remove the activated carbon, concentrating the obtained filtrate, adding ethanol with the mass concentration of 95% into the concentrated solution, fully mixing uniformly, performing centrifugal separation, removing supernatant, collecting precipitate, and drying to obtain the herba Euphorbiae Humifusae extract.
The extraction process of the nine-class nymphaea hybrid extract comprises the following steps:
the water is preferably added into the water and stirred in the step (1) and 5-10 times of the volume of the water is preferably added into the water and stirred for 2 hours; for sufficient extraction, the operations of adding water and stirring can be repeated for a plurality of times on filter residues obtained after adding water and stirring and filtering;
the time for decoloring in the step (2) is 5-60 min; the speed of centrifugal separation in the step (2) is 500-3000 r/min; the drying in the step (2) is preferably vacuum drying at 50-80 ℃;
the ethanol with the mass concentration of 95% added in the step (1) and the step (2) is preferably ethanol with the mass concentration of 95% added in a volume which is 5-10 times that of the corresponding concentrated solution; the concentration in the step (1) and the step (2) refers to the concentration under reduced pressure at the temperature of 20-60 ℃ to 10-40% of the original volume.
The black tiger extract is extracted by the following steps:
the kadsura coccinea extract is prepared by cleaning, drying, grinding, sieving by a 20-200-mesh sieve, adding 90% ethanol, performing reflux extraction, filtering, and performing vacuum drying on the obtained filtrate at 50-85 ℃.
In the extraction process of the black tiger extract, the drying is preferably carried out for 12-24 hours at the temperature of 40-60 ℃; the added 90% ethanol is preferably added with 90% ethanol according to the feed-liquid ratio of 1: 5-15; the reflux extraction time is 1-5 h.
In the extraction process of the black tiger extract, in order to fully extract, the reflux extraction and filtration operations can be repeated for a plurality of times on the filtered filter residue, and then the obtained filtrates are combined and dried in vacuum at 50-85 ℃; the number of repetition is preferably 1-5 times;
a preparation method of the gel containing the Jiupin nymphaea hybrid extract comprises the following steps:
(1) stirring and mixing the component A1 uniformly, adding the component A2, stirring uniformly, adding the component A3, stirring uniformly, heating at 82 +/-5 ℃, keeping the temperature for 3-5min, stirring and cooling to 43 +/-2 ℃ for later use;
(2) adding the component B into the mixture obtained in the step (1), uniformly stirring, and dissolving at normal temperature for later use;
(3) adding the component C into the mixture obtained in the step (2), uniformly stirring, adding the component D, uniformly stirring, adding a mixture of the component E1 and the component E2, and uniformly stirring at normal temperature for later use;
(4) adding the component F into the mixture obtained in the step (3), uniformly stirring, cooling the system to 30 +/-5 ℃, and stirring in vacuum;
(5) and detecting each index to be qualified and discharging.
The stirring speed in the steps (1) to (4) is preferably 400-600 rpm;
in the present invention, the temperature is not specified and is indicated to be carried out at room temperature.
The nine-class nymphaea hybrid is a large-scale nymphaea hybrid for producing perennial root aquatic flowers. The flower is large, the flower color is bright and beautiful, and the flower has nine major color systems of gold, yellow, purple, blue, red, tea, green, red, white and the like, so the flower is named as the nine-grade nymphaea hybrid. The nymphaea hybrid contains rich plant placenta, flavone, nuciferine, crude polysaccharide, oligosaccharide, crude fiber and the like. Researches show that the nine-class nymphaea hybrid extract has good moisturizing effect and is an excellent natural humectant. And secondly, the composition also has the effects of resisting oxidation, relieving allergy and itching, resisting inflammation, resisting cancer and the like.
The black tiger is also named as Kadsura coccinea and Kadsura longepedunculata, and is an evergreen climbing vine plant of Kadsura longepedunculata of Schisandraceae. The Tiger nut extract contains lignans, triterpenes, monoterpenes, sesquiterpenes, steroids, amino acids, etc. Modern medical research shows that the black tiger fruit extract has the functions of resisting oxidation, tumor, virus, liver fibrosis and the like.
Compared with the prior art, the invention has the following advantages and beneficial effects:
the product of the invention has good sedative and anti-allergy effects. The flavone in the water lily extract can inhibit inflammation-related substances, reduce vascular permeability, prevent inflammation diffusion, protect the cell surface from inflammation caused by a large amount of residual substances due to the reproduction of a large amount of microorganisms, enhance the immune system and reduce the occurrence of inflammation. The lignin in the kadsura coccinea extract can inhibit the generation of NO, thereby reducing the degree of inflammation, avoiding the enhancement of the inflammation, and simultaneously having bacteriostatic stability and thermal stability.
The product has good whitening and wrinkle resisting effects. The polysaccharide in the water lily extract has good moisturizing effect, is an excellent natural humectant, and has the functions of resisting oxidation, enhancing immunity, reducing blood fat and the like; the flavone can effectively remove oxygen free radicals in vivo, improve antioxidase activity, and has synergistic effect with vitamin C, so that vitamin C tends to be stable in human tissue. The Tiger nut extract contains abundant vitamin C, can promote the formation of collagen, make skin smooth, white, and elastic, prevent skin wrinkle aging, has antioxidant and antiaging effects, and can increase vitamin C absorption of fibroblast, thereby stimulating the synthesis of collagen; inhibiting the activity of metalloproteinases (MMP-2 and MMP-9) and preventing the degradation of extracellular matrix components; the free radicals are neutralized, so that the antioxidant performance is good; slowing down the wrinkle depth, increasing the skin elasticity and playing the role of resisting wrinkles and preventing wrinkles.
The water lily extract and the black tiger fruit extract are used in a compatible manner, the water lily extract is used as a monarch, the black tiger fruit extract is used as a minister, the effectiveness and the stability of the product can be obviously improved, the skin can fully absorb and utilize effective components in the formula, and epidermal cells can be protected from being invaded by free radicals after long-term use, so that the effects of improving skin quality, moistening the skin, whitening and moisturizing, resisting wrinkles and fading spots, calming and resisting allergy are achieved.
Drawings
FIG. 1 is a photograph of the eyes of volunteers before and after 2 weeks of use of the gel prepared by the example of the present invention.
Detailed Description
The present invention will be described in further detail with reference to examples and drawings, but the embodiments of the present invention are not limited thereto.
The reagents used in the examples are commercially available without specific reference.
The tremella extract is the low molecular weight tremella polysaccharide prepared by the method of example 1 in the Chinese patent publication of CN 201610693617.5-a low molecular weight tremella polysaccharide and the preparation method and application thereof.
The dendrobium officinale stem extract is the ultralow molecular weight dendrobium officinale polysaccharide prepared by the method of the embodiment 1 in the Chinese patent publication "CN 201810743725.9-an ultralow molecular weight dendrobium officinale polysaccharide and the preparation and application thereof".
Examples 1 to 3 and comparative examples 1 to 3
The formulations of examples 1 to 3 and comparative examples 1 to 3 are shown in table 1 below, wherein the part by weight of the jiupin nymphaea hybrid extract in example 1 is 7 parts, and the part by weight of the black tiger extract is 3 parts; in example 2, the nine-class nymphaea hybrid extract is 5 parts by weight, the black tiger extract is 5 parts by weight, and the use amounts of the other components are the same as those in example 1; in example 3, the nine-class nymphaea hybrid extract is 3 parts by weight, the black tiger extract is 7 parts by weight, and the use amounts of the other components are the same as those in example 1; in comparative example 1, the parts by weight of the nine-pin nymphaea hybrid extract are 0 part, the parts by weight of the black tiger extract are 10 parts, and the using amounts of the other components are consistent with those in example 1; in the comparative example 2, the nine-class nymphaea hybrid extract is 10 parts by weight, the black tiger extract is 0 part by weight, and the use amounts of the other components are the same as those in the example 1; in comparative example 3, the nine-pin nymphaea hybrid extract is 0 part by weight, the black tiger extract is 0 part by weight, and the amounts of the other components are the same as those in example 1.
The preparation method of the jipin nymphaea hybrid extract in the examples 1 to 3 and the comparative examples 1 to 3 is as follows: taking fresh nine-class nymphaea hybrid, cleaning, oven drying at 80 deg.C, and homogenizing in tissue triturator to obtain viscous liquid. Adding 7 times volume of distilled water into the homogenate, stirring and extracting for 2h, filtering, and repeatedly extracting the filter residue for 2 times. The filtrates were combined and concentrated at 40 ℃ under reduced pressure to 25% of the original volume. Adding ethanol with the mass concentration of 3 times of that of 95% into the concentrated solution, fully mixing the mixture, filtering the mixture after precipitation, collecting the precipitate, dissolving the precipitate in hot water at 50-80 ℃, adding activated carbon for decoloring for 20min, filtering to remove the activated carbon, concentrating the obtained filtrate at 40 ℃ under reduced pressure to 25% of the original volume, adding ethanol with the mass concentration of 95% of 3 times of that of the concentrated solution, fully mixing the mixture, performing centrifugal separation at the rotating speed of 1000r/min, removing supernatant, collecting the precipitate, and performing vacuum drying at 50-85 ℃ to obtain the product.
The method for preparing Kadsura COCCINEA fruit extract of examples 1 to 3 and comparative examples 1 to 3 comprises: cleaning black tigers, drying at 50 ℃ for 18h, sieving with a 60-mesh sieve, and mixing the materials according to a material-liquid ratio of 1: adding 90% ethanol into the mixture 10, extracting for 3 times with reflux, each time for 2h, filtering the extract, combining the 3 times of filtrates, and vacuum-drying at 50-85 ℃ to obtain the final product.
TABLE 1 formulations of the gels prepared in examples 1-3 and comparative examples 1-3
The preparation steps of examples 1 to 3 and comparative examples 1 to 3 were as follows:
the method comprises the following steps: sequentially adding the component A1, stirring, adding the component A2, stirring, adding the component A3, heating to 82 + -5 deg.C, maintaining the temperature for 3-5min, stirring, and cooling to 43 + -2 deg.C.
Step two: adding the raw material of the component B, stirring uniformly, and dissolving at normal temperature for later use.
Step three: adding the component C, stirring, sequentially adding the component D, stirring, adding the component E (E1, E2, and mixing), and stirring at room temperature.
Step four: adding the F component prefabricated liquid and stirring uniformly.
Step five: cooling the system to 30 +/-5 ℃, and stirring in vacuum.
Step seven: and detecting each index to be qualified and discharging.
Wherein the stirring speed is 400-600 rpm.
And (3) testing the efficacy:
testing the population: 60 female volunteers aged 25-60 years were recruited with the following main symptoms: the face has allergic red spots and pruritus and obvious wrinkles. Volunteers were randomly divided into 6 groups of 10 persons each.
Measuring environment: the test environment temperature (22 +/-1) DEG C and the humidity (50 +/-5)%, and the average value is taken.
The using method comprises the following steps: after each group of volunteers thoroughly cleansed the skin every morning and evening, the skin care products prepared in examples 1 to 3 and comparative examples 1 to 3 were uniformly applied to the face, respectively, and the test site was used for 2 weeks without using any cosmetic product other than the test sample during the test period.
First, calm and anti-allergy efficacy assessment
The test method comprises the following steps: and performing questionnaire survey on the test population.
Evaluation criteria: the effect is obvious, the pruritus is obviously relieved or disappeared, the secondary skin lesions are basically eliminated, and the itchy skin is obviously improved; effective-reduction of itching, improvement of secondary lesions, improvement of itchy skin; ineffectiveness-no improvement in itching, no reduction in secondary skin lesions, no improvement in pruritic skin.
TABLE 2 comparison of sedative and anti-allergy effects of the gels prepared in examples 1 to 3 and comparative examples 1 to 3
As can be seen from Table 2, the allergic phenomenon was significantly improved after 2 weeks using the skin care product of the present invention. The experimental result shows that the condensation containing the nine-class nymphaea hybrid extract is absorbed through the skin, so that the effective components are absorbed to the dermis of the skin from the surface of the skin, the skin quality is improved, the skin is moistened, the problem that the skin has allergic red spot and pruritus is effectively solved, and the condensation has good sedative and anti-allergy effects.
Second, evaluation of whitening and anti-wrinkle efficacy
Whitening effect
The test method comprises the following steps: the melanin content of the face skin is measured by a skin pigment instrument respectively one day before the product is used and 2 weeks after the product is continuously used. The higher the measurement, the higher the melanin content in the skin. The higher the average melanin reduction rate, the more remarkable the whitening effect.
Calculating the formula: melanin reduction rate (black pigment content before 1 day of use-black pigment content 14 days after continuous use)/black pigment content 1 day before use 100%.
The average melanin reduction rate is the sum of/10 of the melanin reduction rates of each group.
TABLE 3 whitening efficacy test data Table for the gels prepared in examples 1 to 3 and comparative examples 1 to 3
As can be seen from Table 3, the gel containing the Jiupin nymphaea hybrid extract can effectively inhibit the generation of melanin, and has obvious effect.
(II) anti-wrinkle efficacy
The test method comprises the following steps: and performing questionnaire survey on the test population.
Evaluation criteria: the effect is obvious, the skin is smooth and elastic, wrinkles are obviously reduced, and small fine lines disappear; effective-skin is smoother, elasticity is improved, small fine lines are obviously faded, but wrinkles still exist; non-effective-small lines, wrinkles still exist, even with a tendency to deepen.
TABLE 4 comparison of anti-wrinkle Effect of the gels prepared in examples 1 to 3 and comparative examples 1 to 3
As can be seen from Table 4, the prepared condensation containing the Jiupin nymphaea hybrid extract can obviously reduce fine lines, make skin texture more uniform and delay skin aging.
One of the volunteers (No. 1902001E01) was selected, and the photo images of the under-eye before use and after 2 weeks use of the gel containing the Euphorbia lathyris extract prepared in example 1 of the present invention are shown in FIG. 1, and as can be seen from FIG. 1, the percentage of fine lines under the eye is significantly reduced and the skin texture is more uniform after 2 weeks use of the gel containing the Euphorbia lathyris extract prepared in example 1 of the present invention.
As can be seen from tables 2-4, the components of the invention have synergistic effect. The gel containing the nine-class nymphaea hybrid extract has obvious effects of eliminating wrinkles, smoothing skin, calming, anti-allergy and whitening, has better effect after long-term use, can make the skin smooth and compact and rich in luster, can remove color spots, and has better whitening effect. Meanwhile, according to the reaction of volunteers, no irritation and allergy phenomena of the product to the testers are found.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.
Claims (8)
1. A gel containing a nine-class nymphaea hybrid extract is characterized by comprising the following components in percentage by mass:
a1 component: 5% of glycerol and 0.05% of xanthan gum;
a2 component: 62.87% of water;
a3 component: 0.2% of carbomer;
the component B comprises: betaine 0.5%, nicotinamide 2%, water 5%;
and the component C is as follows: 0.1 percent of arginine;
and (D) component: 1% of tremella extract, 0.2% of carboxymethyl chitosan, 1% of dendrobium officinale stem extract, 10% of Jiupin nymphaea hybrid extract and 10% of black tiger extract;
e1 component: 0.02% of essence and 0.06% of solubilizer;
e2 component: 10% of water;
and F component: 1% of 1, 3-butanediol and 1% of preservative;
the black tiger extract is extracted by the following steps: cleaning and drying the black cutworm, grinding the dried black cutworm, sieving the ground black cutworm by a sieve of 20 to 200 meshes, adding 90 percent ethanol, performing reflux extraction, filtering the mixture, and drying the obtained filtrate at 50 to 85 ℃ in vacuum to obtain a black cutworm extract;
the nine-class nymphaea hybrid extract is extracted by the following method: (1) taking fresh nine-product nymphaea hybrid, cleaning, drying surface water, adding the surface water into a tissue triturator, homogenizing into a viscous liquid, adding water into the obtained homogenate liquid, stirring and extracting, then filtering, concentrating the obtained filtrate, adding ethanol with the mass concentration of 95%, fully mixing, filtering after precipitation, and collecting precipitate; (2) dissolving the obtained precipitate with hot water at 50-80 ℃, adding activated carbon for decolorization, then filtering to remove the activated carbon, concentrating the obtained filtrate, adding ethanol with the mass concentration of 95% into the concentrated solution, fully and uniformly mixing, performing centrifugal separation, removing supernatant, collecting precipitate, and drying to obtain a nine-class lotus extract;
the tremella extract is prepared by the following method: taking 30kg of dry tremella, pulverizing into powder, sieving with a 60-mesh sieve, adding water according to the mass ratio of 100:1 of liquid to material, adjusting the pH to 10 with NaOH, reacting at 85 ℃ for 4 hours to obtain a crude extract, cooling the crude extract to 50 ℃, adjusting the pH to 5.5, adding 0.15kg of pectin cellulose complex enzyme, reacting for 4 hours, carrying out coarse filtration with gauze, filtering out filter residues, filtering with a ceramic membrane with the interception aperture of 1 mu m to remove impurities to obtain 2470kg of filtrate, concentrating under reduced pressure to one tenth of the original volume to obtain 267kg of thick paste; adding 500mL of 95 wt% ethanol into the thick paste to make the final volume concentration of the ethanol reach 80%, and adding H2O2Enabling the final volume concentration to reach 2%, stirring at normal temperature for 36 hours, reducing the temperature to 4 ℃, standing for 12 hours, centrifuging by using a hanging bag centrifuge at 2000r/min until liquid is completely thrown out, collecting a precipitate part, cleaning the precipitate by using 95% ethanol for 3 times, centrifuging at 2000r/min, recovering the ethanol, and drying the precipitate in a 45 ℃ oven to obtain 7.63kg of low-molecular-weight tremella polysaccharide; wherein the mass ratio of pectinase to cellulase in the pectin cellulose compound enzyme is 1:1, the specification of the pectinase is 3 ten thousand U/g, and the specification of the cellulase is 1 ten thousand U/g;
the extraction method of the dendrobium officinale stem extract comprises the following stepsThe preparation method comprises the following steps: (1) strain activation and expanding culture: dissolving Aspergillus tubingensis strain in sterile water to obtain bacterial suspension, transferring 3 μ L of bacterial suspension onto PDA solid culture medium, streaking, activating, culturing at 30 deg.C for 48 hr to obtain activated Aspergillus tubingensis strain with concentration of 1 × 104CFU/mL; inoculating the activated Aspergillus tubingensis strain into a fermentation shake flask containing a Sabouraud's liquid culture medium at an inoculation amount of 1% for propagation at a culture temperature of 30 ℃ and a rotation speed of 150r/min, and culturing for 48h to obtain an Aspergillus tubingensis fermentation seed solution with a strain concentration of 1 × 107CFU/mL; (2) treating the dendrobium officinale raw materials: weighing 4kg of fresh Dendrobium officinale, crushing with a crusher, putting the juice and the residues into a 100L fermentation tank, injecting 96kg of Sabouraud's liquid culture medium to make the mass fraction of Dendrobium officinale 4.0%, sealing the fermentation tank, heating to 120 ℃, keeping for 30min for sterilization, and cooling to room temperature; (3) fermentation culture: adding 1% volume fraction of Aspergillus tubingensis fermentation seed liquid into a Sabouraud's liquid culture medium containing 4% of Dendrobium officinale, adjusting the pH of the liquid in a fermentation tank to 5.4 by using a citric acid-sodium citrate buffer solution, fermenting for 3 days at 30 ℃ and a rotation speed of 150r/min to obtain a fermentation liquid, heating the fermentation liquid to 120 ℃, and keeping for 30min to finish the inactivation treatment of microorganisms and biological enzymes; (4) polysaccharide purification: after the fermentation liquor is cooled, adding decolored activated carbon accounting for 1.0 percent of the mass of the fermentation liquor into the fermentation tank, standing and adsorbing for 2 hours, filtering by using a plate-and-frame filter press, and sequentially filtering the filtrate by using polypropylene microfiltration membrane filter columns of 10 microns, 5.0 microns and 2.5 microns to remove fine impurities to obtain 87.5kg of filtrate; (5) and (3) polysaccharide drying: concentrating the filtrate by evaporation under reduced pressure at 50 deg.C under-0.10 MPa to 1/10% of original volume to obtain herba Dendrobii polysaccharide extract 9 kg; adding maltodextrin excipient into the dendrobium polysaccharide extract, wherein the mass ratio of maltodextrin to dendrobium polysaccharide extract is 1:100, uniformly dispersing, circularly homogenizing by using a high-pressure homogenizer at the homogenizing pressure of 35MPa and the temperature of 25 ℃ for 10 times, spray drying the homogenized product, and obtaining 1.20kg of dendrobium polysaccharide light yellow powder, wherein the air inlet temperature is 140 ℃, the air inlet pressure is 40MPa, and the air outlet temperature is 70 ℃, and the pure dendrobium polysaccharide is pureThe degree was 98%.
2. The gel containing the jiupin nymphaea hybrid extract according to claim 1, wherein:
the weight percentage of the nine-class nymphaea hybrid extract is 7 percent, and the weight percentage of the black tiger extract is 3 percent.
3. The gel containing the extract of Asparagus racemosus according to claim 1, wherein the extract comprises the following components in percentage by weight:
the composition comprises 5% of a nine-class nymphaea hybrid extract and 5% of a black tiger extract in percentage by mass.
4. The gel containing the jiupin nymphaea hybrid extract according to claim 1, wherein:
the weight percentage of the nine-class nymphaea hybrid extract is 3 percent, and the weight percentage of the black tiger extract is 7 percent.
5. The gel containing the Euphorbia pepora extract according to any one of claims 1 to 4, wherein:
the solubilizer is 1, 3-butanediol; the preservative is PHL.
6. The gel containing the jiupin nymphaea hybrid extract according to claim 1, wherein:
the extraction process of the nine-class nymphaea hybrid extract comprises the following steps:
the step (1) of adding water and stirring refers to adding water with the volume of 5-10 times of that of the mixture and stirring and extracting for 2 hours;
the time for decoloring in the step (2) is 5-60 min; the speed of centrifugal separation in the step (2) is 500-3000 r/min; the drying in the step (2) refers to vacuum drying at 50-80 ℃;
the ethanol with the mass concentration of 95% added in the step (1) and the step (2) is added with the ethanol with the mass concentration of 95% in a volume which is 5-10 times that of the corresponding concentrated solution;
the concentration in the step (1) and the step (2) refers to the concentration under reduced pressure at the temperature of 20-60 ℃ to 10-40% of the original volume.
7. The gel containing the jiupin nymphaea hybrid extract according to claim 1, wherein:
in the extraction process of the black tiger extract, drying is carried out for 12-24 hours at 40-60 ℃; the 90% ethanol is added according to the feed-liquid ratio of 1: 5-15; the reflux extraction time is 1-5 h.
8. A method for preparing a gel containing a Euphorbia pepora extract according to any one of claims 1 to 7, characterized by comprising the following steps:
(1) stirring and mixing the component A1 uniformly, adding the component A2, stirring uniformly, adding the component A3, stirring uniformly, heating at 82 +/-5 ℃, keeping the temperature for 3-5min, stirring and cooling to 43 +/-2 ℃ for later use;
(2) adding the component B into the mixture obtained in the step (1), uniformly stirring, and dissolving at normal temperature for later use;
(3) adding the component C into the mixture obtained in the step (2), uniformly stirring, adding the component D, uniformly stirring, adding a mixture of the component E1 and the component E2, and uniformly stirring at normal temperature for later use;
(4) adding the component F into the mixture obtained in the step (3), uniformly stirring, cooling the system to 30 +/-5 ℃, and stirring in vacuum;
(5) detecting each index to be qualified and discharging;
the stirring speed in the steps (1) to (4) is 400-600 rpm.
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| CN111494500B (en) * | 2020-05-15 | 2022-05-31 | 湖南环境生物职业技术学院 | Black tiger pericarp extract and preparation method and application thereof |
| CN113750017A (en) * | 2020-10-09 | 2021-12-07 | 贵州省中国科学院天然产物化学重点实验室(贵州医科大学天然产物化学重点实验室) | Method for preparing dendrobium nobile skin care product with whitening effect |
| CN113262190B (en) * | 2021-06-04 | 2022-08-23 | 浙江大学 | External composition for resisting skin aging and application thereof |
| CN114917160B (en) * | 2022-06-06 | 2023-09-08 | 青蛙王子（福建）婴童护理用品有限公司 | A flos Nelumbinis ferment with antioxidant, antiinflammatory and repairing effects, and its preparation method and application |
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