CN113694142A

CN113694142A - Preparation method of anti-aging traditional Chinese medicine formula by utilizing probiotic fermentation

Info

Publication number: CN113694142A
Application number: CN202110995653.8A
Authority: CN
Inventors: 徐晓英; 段礼新; 吴杨
Original assignee: Individual
Current assignee: Individual
Priority date: 2021-08-27
Filing date: 2021-08-27
Publication date: 2021-11-26

Abstract

The invention relates to a preparation method of an anti-aging traditional Chinese medicine formula by utilizing probiotic fermentation, which comprises the steps of raw material pretreatment, enzymolysis, homogenization and fermentation, wherein lactobacillus is added into a homogenized material obtained by the enzymolysis and other raw materials for fermentation; the lactobacillus is selected from lactobacillus casei (l.casei), lactobacillus bulgaricus (l.bulgaricus), lactobacillus acidophilus (l.acidophilus) or lactobacillus thermophilus (l.thermophilus). The invention adopts lactic acid bacteria to carry out traditional Chinese medicine fermentation on ginseng root extract and raw materials of astragalus, dendrobium, semen cuscutae, schisandra chinensis and radix ophiopogonis, and no fermentation method is reported before; the effects of tonifying qi and other raw materials of the ginseng are combined to achieve the effect of tonifying qi and blood; the embodiment further performs enzymolysis on the ginseng extract, thereby being beneficial to the degradation and absorption of macromolecules; compared with the ginseng extract, the method does not need organic solvents such as ethanol and the like, is environment-friendly, and does not irritate the skin.

Description

Preparation method of anti-aging traditional Chinese medicine formula by utilizing probiotic fermentation

Technical Field

The invention relates to the technical field of bioengineering, in particular to a preparation method of an anti-aging traditional Chinese medicine formula by utilizing probiotic fermentation.

Background

Aging refers to the natural phenomenon that after an organism is matured under normal conditions, the organization structure of the organism gradually undergoes degenerative change along with the increase of age, the functions of organs of the organism are reduced, the self-stabilizing capability of the internal environment is weakened, the resistance of damage factors of the internal environment and the external environment is reduced, and the organism tends to die. Aging due to age is an inevitable phenomenon. Other factors causing aging include health factors, environmental factors, mental factors, nutritional factors, lifestyle habits, endocrine disorders, improper medication, and other environmental or individual factors. The effective components of the plants are used as a means for resisting aging of human beings.

The release of active ingredients in cytoplasm of the cells is blocked because the plant cells have cell walls with compact structures; secondly, the molecular weight of the effective components of the traditional Chinese medicine is generally larger, so that the effective components are not easy to break through the skin barrier and be absorbed by the human body. The utilization rate of effective components of the traditional Chinese medicinal materials can be greatly improved by microbial fermentation treatment, because microorganisms can generate a plurality of extracellular enzymes such as cellulase, pectinase and the like in the metabolic process, and the extracellular enzymes can decompose cell walls of plant cells, so that the cells of the traditional Chinese medicinal materials are broken, and the effective components are exposed. In addition, various enzymes generated by microbial metabolism can reduce the molecular weight of effective components, and simultaneously remove various macromolecular impurities, so that the enzymes can be better absorbed and utilized by human bodies.

Probiotic bacteria refer to microorganisms beneficial to body health, and as of 2019, probiotic bacteria species available in the food field including 35 species or subspecies are published in China on "list of probiotic bacteria species available for health food" (No. 2001/84 by law), "list of fungal bacteria species available for health food" (No. 2001/84 by law), "list of bacteria species available for food" (No. 2010) and "list of bacteria species available for infant food" (No. 2011/25 by Ministry of health).

The invention discloses a Chinese herbal compound essential oil with anti-aging effect and a preparation method thereof, and the invention is CN106943313A, and discloses the Chinese herbal compound essential oil with anti-aging effect, wherein the active ingredients of the Chinese herbal compound essential oil are prepared from the following raw materials in parts by weight: 1-10 parts of cacumen biotae, 1-10 parts of madder, 1-10 parts of ligusticum wallichii, 1-10 parts of angelica sinensis and 1-10 parts of saffron crocus. The combination of the medicines can moisten and nourish skin, reduce free radical damage and promote metabolism, and has good anti-aging effect. The technical scheme utilizes the measures of freezing, puffing, supercritical extraction and the like to obtain the effective essential oil in the plant material.

The invention of publication No. CN108743486A is a compound with an anti-aging function and an application thereof, and the disclosure content comprises that the compound comprises Heteropanar total triterpenes, wherein the Heteropanar total triterpenes comprise Heteropanar triterpenoid saponins and Heteropanar triterpenic acid, and the mass ratio of the Heteropanar triterpenoid saponins to the Heteropanar triterpenic acid is (1-5) to (1-8). The compound with the anti-aging function provided by the invention synergistically compounds the Heteropanar triterpenoid saponin and the Heteropanar triterpenoid acid which are separated from the rhizome and the branches and leaves of Heteropanar, and compared with singly using the triterpenoid saponin or the triterpenoid acid, the compound with the anti-aging function can more effectively promote the synthesis of collagen protein and further increase collagen fibers. The technical scheme adopts a compounding scheme of effective active substances of Heteropanar triterpenoid saponins and Heteropanar triterpenoid acid from plant Heteropanar, and the composition is used in cosmetics such as anti-aging cream.

The invention of the publication No. CN1033365C relates to a composition taking hydrated sheet-shaped lipid phase or liposome as a main component, which contains scutellaria baicalensis extract or at least one component thereof, and a cosmetic or medicament, in particular to a composition having antiallergic, anti-inflammatory or anti-aging effects on skin. The composition is characterized in that said hydrated lamellar lipid phase or said liposomes contain at least partially an extract of Scutellaria or at least one active substance isolated from said extract or obtained by chemical synthesis, said active substance consisting essentially of: baicalein, 2' -hydroxy-baicalein, 5, 6, 7-trihydroxyflavone (Begatine), neo5, 6, 7-trihydroxyflavone, oropteridine (oxoridine) and baicalin. The composition can be used for preparing cosmetic or pharmaceutical composition, especially composition with antiallergic, antiinflammatory or antiaging effects on skin.

The ginseng has unique nourishing and beautifying effects, is a traditional Chinese beautifying and skin-care product, has consistent and approved whitening, anti-wrinkle and anti-aging effects, is applied to cosmetics from the 20 th century and the 80 th century, such as various ginseng cosmetics such as ginseng moisturizer, ginseng whitening cream, ginseng mask and the like, and becomes the most widely used plant cosmetic raw material at present. The ginseng whitening cream contains ginsenoside Rb2, Rc, Rd, Re, Rg1, Rg2, Rh1 and notoginsenoside R2 (gold and precious and modern traditional Chinese medicine, 2009). When the dosage concentration of the ginsenoside Rg1 is 10-80 mug/mL, the proliferation of melanocyte can be inhibited, and the synthesis of melanin can be reduced (Zhao Bao Sheng, ginseng research, 2011). Ginsenoside Rb and ginsenoside Rd can inhibit matrix metalloproteinase MMP-1 and MMP-3 expression (Dumeng, J. dermatology, 2010). Researches show that in a UVB-induced fibroblast photoaging model, the ginseng extract can simultaneously regulate MAPKs and NF-kB signal pathways, inhibit abnormal secretion of MMP-l and MMP-13 and achieve the photoaging resistance effect (Yong, NutrRes, 2012).

The release of active ingredients in cytoplasm of the cells is blocked because the plant cells have cell walls with compact structures; and the molecular weight of the effective components is generally larger, so that the effective components are not easy to break through the skin barrier and be absorbed by the human body. The traditional Chinese medicinal materials are subjected to microbial fermentation treatment, so that the utilization rate of effective components can be greatly improved, and the microorganisms can generate various extracellular enzymes such as cellulase, pectinase and the like in the metabolic process, and can decompose cell walls of plant cells to break the cells of the traditional Chinese medicinal materials, so that the effective components are exposed. Therefore, the method and the product for exploring effective fermentation means and effectively improving the anti-aging effective components in the plants are valuable exploration work in reality.

Disclosure of Invention

According to the needs of the prior art, one object of the present invention is to provide a method for preparing an anti-aging traditional Chinese medicine formula by using probiotic fermentation.

The preparation method of the anti-aging traditional Chinese medicine formula by utilizing probiotic fermentation is realized by the following technical scheme.

According to the technical scheme, the used plant raw materials comprise astragalus membranaceus, ginseng, dendrobe, semen cuscutae, schisandra chinensis and radix ophiopogonis; wherein, calculated by weight portion, 18-22 portions of astragalus root, 4-6 portions of ginseng, 4-6 portions of dendrobium stem, 18-22 portions of dodder, 18-22 portions of schisandra fruit and 14-16 portions of ophiopogon root.

Preferably, the plant raw materials comprise, by weight, 19-21 parts of astragalus membranaceus, 4.5-5.5 parts of ginseng, 4.5-5.5 parts of dendrobium, 19-21 parts of semen cuscutae, 19-21 parts of schisandra chinensis and 14.5-15.5 parts of radix ophiopogonis.

More preferably, the plant raw materials comprise, by weight, 20 parts of astragalus membranaceus, 5 parts of ginseng, 5 parts of dendrobium, 20 parts of semen cuscutae, 20 parts of schisandra chinensis and 15 parts of radix ophiopogonis.

The method in the technical scheme of the invention comprises the steps of pretreating dry ginseng or fresh ginseng, and then digesting the pretreated dry ginseng or fresh ginseng by enzymolysis; then carrying out high-pressure homogenization treatment by using micro-jet equipment, and simultaneously carrying out cooling treatment on a homogenizer by using cooling water so as to fully release active ingredients in ginseng cells under the low-temperature condition; purifying the homogenized raw material; then, adding other raw materials; and adding lactobacillus to perform fermentation treatment to obtain a target-containing fermentation liquid.

Further, the lactobacillus according to the present invention is selected from lactobacillus casei (l.casei), lactobacillus bulgaricus (l.bulgaricus), lactobacillus acidophilus (l.acidophilus) or lactobacillus thermophilus (l.thermophilus), wherein lactobacillus acidophilus or lactobacillus thermophilus is preferred.

Specifically, the method according to the technical scheme of the invention comprises the following steps:

step (1), raw material pretreatment: comprises processing dried Ginseng radix or fresh Ginseng radix material into appropriate size to reach suitable sample size. Soaking the pulverized ginseng material in a certain extraction solvent, wherein the extraction solvent is water, and preferably the extraction solvent is purified water or deionized water.

Further, the weight ratio of the human participated in the water is 1: 6-10 times of purified water, preferably 6 times; adjusting the pH value to 7-7.5.

Step (2), enzymolysis step: adding endoprotease and cellulase into the ginseng material, performing enzymolysis for 3-4h, stirring regularly during the enzymolysis process, and inactivating the enzyme of the obtained enzymolysis liquid after the enzymolysis is finished.

Further, in the enzymolysis step, the added endoprotease accounts for 0.5-0.7% of the weight of the ginseng raw material, and the added cellulase accounts for 0.1-0.2% of the weight of the ginseng raw material, so that enzymolysis is carried out. In the enzymolysis process, stirring is carried out for 5-10 minutes every 1 hour, so that the enzymolysis is uniformly and fully carried out. The cell wall of the ginseng is further destroyed by the action of cellulase and endoprotease.

Step (3), homogenizing: a micro-jet high-pressure homogenizer is adopted, and the feeding is pumped by a booster pump or a homogenizer plunger pump;

further, material pressurization: pressurizing the material in a plunger booster pump to a set pressure value of 30-40 MPa, preferably 35-38 MPa, more preferably 35.8 MPa; the ginseng homogenized material after the homogenization treatment is collected into a discharging container.

Further, in the process, the temperature of the material subjected to high-pressure micro-jet treatment is instantaneously raised, and the material needs to be cooled by a heat exchanger, wherein in the technical scheme of the invention, the real-time temperature control range is set to be 38-42 ℃, and is preferably 38.8-40 ℃;

step (4) fermentation treatment

Adding the crushed other raw materials and water into the homogenized material obtained in the step, and adding lactobacillus for fermentation treatment. The other raw materials comprise astragalus, dendrobium, dodder, schisandra and ophiopogon root, and the raw materials are crushed into 80-120 meshes; the volume ratio of the homogenized material to other raw materials to water is 1: 10-20; the addition amount of lactobacillus is 5-8% of the dry matter weight of all raw materials;

wherein, the culture of the lactobacillus comprises the following steps:

(1) inoculating lactobacillus to a liquid fermentation culture medium for culturing to obtain a seed solution of the strain.

(2) Inoculating the seed liquid of the strain into a culture medium for fermentation culture.

(3) And (3) drying the fermented material obtained in the step (2).

Further, after the lactobacillus is subjected to seed culture, the lactobacillus is subjected to amplification culture; wherein, the enlarged culture is firstly fermented and cultured for 36h at 30 ℃, and then is continuously fermented and cultured for 36h at 32 ℃;

in the step (1), after the lactobacillus is subjected to seed culture and amplification culture, the thallus density is more than 30g wet thallus/L.

In the step (1), the lactobacillus is cultured in the seed culture medium and the seed culture method, which are conventional lactobacillus seed culture medium and conventional lactobacillus seed culture method, for example, the seed culture medium preferably has the following formula: 60g/L glucose, 2.0g/L urea, KH₂PO₄0.6g/L, 30g/L ammonium chloride, MgSO₄7H₂O0.5g/L, yeast extract 0.5g/L, liquid paraffin 20g/L, solvent is water; the seed culture method is preferably: lactobacillus seeds are inoculated into a seed culture medium and cultured for 24 hours at the temperature of 30 ℃ and under the condition of 200 r/min.

In the step (1), the lactobacillus is subjected to scale-up culture, wherein a culture medium of the lactobacillus is a conventional fermentation scale-up culture medium, and for example, the scale-up culture medium preferably has the following formula: 60g/L glucose, 2.0g/L urea, KH₂PO₄0.6g/L, 30g/L ammonium chloride, MgSO₄7H₂O0.5g/L, yeast extract 0.5g/L, liquid paraffin 20g/L, and water as solvent. The expansion culture method adopts a variable temperature culture mode, and preferably comprises the following steps: inoculating the seed liquid into a fermentation amplification culture medium according to the volume percentage of 10%, performing fermentation culture for 48h at the temperature of 33 ℃ and the speed of 200r/min, then increasing the temperature to 35 ℃, and continuing to culture for 48h, wherein the thallus density is more than or equal to 30g of wet thallus/L. After the enlargement cultureFiltering and recovering the thalli, and preserving at a low temperature of 4 ℃ for later use. When used, a lactic acid bacterium suspension was prepared with 10mmol/L phosphate buffer solution (pH 8.0).

Further, in the step (2), the lactobacillus is subjected to amplification culture, and stimulation culture is carried out by using an ultrasonic biological stimulation growth instrument, wherein the ultrasonic biological stimulation growth instrument has the treatment parameters of ultrasonic frequency of 30kHz, power of 10W, ultrasonic time of 10s and interval time of 20s, and ultrasonic stimulation is carried out for 30 minutes in total.

Compared with the prior art, the invention has the following remarkable advantages and effects: 1. the invention adopts lactic acid bacteria to carry out traditional Chinese medicine fermentation on ginseng root extract and raw materials of astragalus, dendrobium, semen cuscutae, schisandra chinensis and radix ophiopogonis, and no fermentation method is reported before. 2. Combines the effects of the ginseng for tonifying qi and other raw materials to achieve the effect of tonifying qi and blood. 3. The embodiment further performs enzymolysis on the ginseng extract, and is beneficial to the degradation and absorption of macromolecules. 4. Compared with the ginseng extract, the method does not need organic solvents such as ethanol and the like, is environment-friendly, and does not irritate the skin.

In order to make the purpose and technical solution of the embodiments of the present invention clearer, the technical solution of the embodiments of the present invention will be clearly and completely described below with reference to the implementation examples of the embodiments of the present invention. It is to be understood that the embodiments described are only a few embodiments of the present invention, and not all embodiments. All other embodiments, which can be made by a person skilled in the art without inventive effort based on the described embodiments of the invention, fall within the scope of protection of the invention.

Drawings

FIG. 1 is a schematic flow diagram of the process of the present invention;

FIG. 2 is the total saponin absorption spectrum of the fermentation broth ginsenoside and the reference solution.

Detailed description of the preferred embodiments

The technical solution of the present invention is further illustrated by the following specific examples. In the following embodiments, the raw materials are all commercial products purchased from the relevant manufacturing enterprises or commercial departments, unless otherwise specified. The micro-jet high-pressure homogenizer or the micro-jet high-pressure homogenizer can obtain the homogenizing pressure of 20MPa to 210 MPa.

Example 1: preparation of the Strain to be inoculated

The culture of lactobacillus comprises the following steps:

(3) And (3) drying the fermented material obtained in the step (2).

After the lactobacillus is subjected to seed culture, carrying out amplification culture; wherein, the enlarged culture is firstly fermented and cultured for 36h at 30 ℃, and then is continuously fermented and cultured for 36h at 32 ℃;

in the step (1), the lactobacillus is cultured by the seed culture medium and the seed culture medium in the seed culture method according to the following formula: 60g/L glucose, 2.0g/L urea, KH₂PO₄0.6g/L, 30g/L ammonium chloride, MgSO₄7H₂O0.5g/L, yeast extract 0.5g/L, liquid paraffin 20g/L, solvent is water; the seed culture method comprises the following steps: lactobacillus seeds are inoculated into a seed culture medium and cultured for 24 hours at the temperature of 30 ℃ and under the condition of 200 r/min.

In the step (1), the lactobacillus is subjected to amplification culture, and the culture medium is an amplification culture medium formula of the lactobacillus as follows: 60g/L glucose, 2.0g/L urea, KH₂PO₄0.6g/L, 30g/L ammonium chloride, MgSO₄7H₂O0.5g/L, yeast extract 0.5g/L, liquid paraffin 20g/L, and water as solvent. The amplification culture method adopts a variable temperature culture mode, the seed liquid with the volume percentage of 10 percent is inoculated into a fermentation amplification culture medium, the fermentation culture is carried out for 48 hours under the conditions of 33 ℃ and 200r/min, then the temperature is increased to 35 ℃, the culture is continued for 48 hours, and the thallus density is more than or equal to 30g wet thallus/L. After the expanded culture, the cells were recovered by filtration and stored at a low temperature of 4 ℃ for further use. When used, a lactic acid bacterium suspension was prepared with 10mmol/L phosphate buffer solution (pH 8.0).

In the step (2), the lactobacillus is subjected to amplification culture, an ultrasonic biological stimulation growth instrument is used for stimulation culture, the processing parameters of the ultrasonic biological stimulation growth instrument are ultrasonic frequency of 30kHz, power of 10W, ultrasonic time of 10s and interval time of 20s, and ultrasonic stimulation is carried out for 30 minutes in total.

Example 2: dried Ginseng radix raw material

This embodiment 2 specifically includes the following steps:

(1) pretreatment of raw materials: selecting dried ginseng rhizome planted for more than 5 years 0.1 kg, and pulverizing to 200-300 mesh. Adding 6 times of purified water for soaking, and adjusting pH to 7.0.

(2) An enzymolysis step: adding endoprotease 0.5 wt% of Ginseng radix and cellulase 0.1 wt% of Ginseng radix for enzymolysis for 3 hr, and stirring for 5 min every 1 hr during enzymolysis to allow enzymolysis to proceed uniformly and sufficiently.

(3) Homogenizing: a micro-jet high-pressure homogenizer is adopted, and the feeding is pumped by a booster pump or a homogenizer plunger pump; material pressurization: pressurizing the material in a plunger booster pump to a set pressure value of 38 Mpa; the pressurized material is in ultramicro homogeneous state in the micro-jet high-pressure homogenizer, and the homogenized ginseng material is collected in a discharge container. In the process, the material subjected to high-pressure micro-jet treatment is cooled by a heat exchanger, and in the embodiment, the real-time temperature control temperature is set to be 40 ℃;

4) fermentation treatment:

adding the crushed other raw materials and water into the homogenized material obtained in the step, and adding lactobacillus for fermentation treatment. The other raw materials comprise 0.4 kg of astragalus, 0.1 kg of dendrobium, 0.4 kg of semen cuscutae, 0.4 kg of schisandra chinensis and 0.3 kg of radix ophiopogonis, and the raw materials are crushed into 80-120 meshes; the volume ratio of the homogenized material to other raw materials to water is 1: 10-20; the addition amount of lactobacillus is 90 g; the lactobacillus is lactobacillus acidophilus;

fermentation culture: the culture process needs a 10L fermentation tank, and adopts an inoculation mode of feeding liquid seeds into the tank. The pH value can be adjusted to 6.5-8.0 by 0.5N-1NHCl and 0.5N-1NNaOH or 10% -20% ammonia water in the whole fermentation process. Larger aeration volumes were used after inoculation.

Example 3: fresh ginseng raw material

This embodiment 3 specifically includes the following steps:

(1) pretreatment of raw materials: selecting 0.3 kg of fresh ginseng roots planted for more than 5 years, adding 5 times of purified water, and adjusting the pH value to 7.5; homogenizing, pulverizing, and sieving with 200-300 mesh sieve.

(3) Homogenizing: a micro-jet high-pressure homogenizer is adopted, and the feeding is pumped by a booster pump or a homogenizer plunger pump; character material pressurization: pressurizing the material in a plunger booster pump to a set pressure value of 35.8 Mpa; the pressurized material is in ultramicro homogeneous state in the micro-jet high-pressure homogenizer, and the homogenized ginseng material is collected in a discharge container. In the process, the material subjected to high-pressure micro-jet treatment is cooled by a heat exchanger, and in the embodiment, the real-time temperature control temperature is set to be 40 ℃;

4) fermentation treatment:

adding the crushed other raw materials and water into the homogenized material obtained in the step, and adding lactobacillus for fermentation treatment. The other raw materials comprise 0.2 kg of astragalus, 0.05 kg of dendrobium, 0.2 kg of semen cuscutae, 0.2 kg of schisandra chinensis and 0.15 kg of radix ophiopogonis, and the raw materials are crushed into 80-120 meshes; the volume ratio of the homogenized material to other raw materials to water is 1: 10-20; the addition amount of lactobacillus is 80 g; the lactobacillus is lactobacillus thermophilus;

Referring to FIGS. 1-2, FIG. 1 is a schematic flow diagram of a method of the present invention; FIG. 2 is the total saponin absorption spectrum of the fermentation broth ginsenoside and the reference solution. The control was prepared as in example 2 without the fermentation step.

The above description further describes a specific embodiment of the present invention with reference to specific examples, which are intended for the detailed description of the present invention and are not intended to limit the present invention. The above-mentioned embodiments are merely descriptions of the preferred embodiments of the present invention, and do not limit the technical concept and the protection scope of the present invention, and various modifications and improvements made to the technical concept by those skilled in the art without departing from the design concept of the present invention shall fall within the protection scope of the present invention.

Claims

1. A preparation method of an anti-aging traditional Chinese medicine formula by utilizing probiotic fermentation is characterized in that the raw materials of the method comprise: astragalus, ginseng, dendrobium, dodder, schisandra and ophiopogon root, wherein the method comprises the following steps:

step (1), raw material pretreatment: comprises processing dried Ginseng radix or fresh Ginseng radix material into appropriate size to reach suitable sample size. Soaking the pulverized ginseng material in an extraction solvent, wherein the extraction solvent is water, and preferably the extraction solvent is purified water or deionized water;

step (2), enzymolysis step: adding endoprotease and cellulase into the ginseng material, performing enzymolysis for 3-4h, stirring regularly during the enzymolysis process, and inactivating the enzyme of the obtained enzymolysis liquid after the enzymolysis is finished;

step (4), fermentation treatment;

adding the crushed other raw materials and water into the homogenized material obtained in the step, and adding lactobacillus for fermentation treatment; the other raw materials comprise astragalus, dendrobium, dodder, schisandra and ophiopogon root, and the raw materials are crushed into 80-120 meshes.

2. The preparation method of the anti-aging traditional Chinese medicine formula by utilizing probiotic fermentation according to claim 1, characterized in that the raw materials comprise, by weight, 18-22 parts of astragalus membranaceus, 4-6 parts of ginseng, 4-6 parts of dendrobe, 18-22 parts of semen cuscutae, 18-22 parts of schisandra chinensis and 14-16 parts of radix ophiopogonis.

3. The preparation method of the anti-aging traditional Chinese medicine formula by utilizing probiotic fermentation according to claim 2, characterized in that the raw materials comprise, by weight, 19-21 parts of radix astragali, 4.5-5.5 parts of ginseng, 4.5-5.5 parts of dendrobe, 19-21 parts of semen cuscutae, 19-21 parts of schisandra chinensis and 14.5-15.5 parts of radix ophiopogonis.

4. The preparation method of the anti-aging traditional Chinese medicine formula by utilizing probiotic fermentation according to claim 3, characterized in that the raw materials comprise, by weight, 20 parts of radix astragali, 5 parts of ginseng, 5 parts of dendrobe, 20 parts of semen cuscutae, 20 parts of schisandra chinensis and 15 parts of radix ophiopogonis.

5. The preparation method of the anti-aging traditional Chinese medicine formula by utilizing probiotic fermentation according to any one of claims 1 to 4, wherein in the step (1) of the method, the weight ratio of the ginseng to the purified water is 1: 6-10 times, and adjusting the pH value to 7-7.5.

6. The method for preparing anti-aging traditional Chinese medicine fermentation liquor by using probiotics according to any one of claims 1 to 4, wherein in the step (2), the enzymolysis is carried out by adding endoprotease 0.5 to 0.7 percent of the weight of the ginseng raw material and cellulase 0.1 to 0.2 percent of the weight of the ginseng raw material. In the enzymolysis process, stirring is carried out for 5-10 minutes every 1 hour, so that the enzymolysis is uniformly and fully carried out. The cell wall of the ginseng is further destroyed by the action of cellulase and endoprotease.

7. The method according to any one of claims 1 to 4, wherein in the step (1), the lactobacillus is enlarged and cultured by using an ultrasonic biostimulation growth instrument, and the ultrasonic biostimulation growth instrument is used for treating parameters of 30kHz, 10W of power, 10s of ultrasonic time and 20s of interval time for 30 minutes of ultrasonic stimulation.

8. The method for preparing the anti-aging traditional Chinese medicine formula by utilizing probiotic fermentation according to any one of claims 1 to 4, characterized in that the step of homogenizing treatment comprises the following steps of: pressurizing the material in a plunger booster pump to a set pressure value of 30-40 MPa, preferably 35-38 MPa, more preferably 35.8 MPa; collecting the homogenized ginseng material into a discharging container; the material treated by the high-pressure micro-jet flow has the phenomenon of instantaneous temperature rise, and needs to be cooled by a heat exchanger, and the real-time temperature control range is set to be 38-42 ℃, and is preferably 38.8-40 ℃.

9. The method for preparing an anti-aging traditional Chinese medicine formula by utilizing probiotic fermentation according to any one of claims 1 to 4, characterized in that in the fermentation treatment step, the volume ratio of the homogenized material and other raw materials to water is 1: 10-20; the addition amount of lactobacillus is 5-8% of the dry matter weight of all raw materials.

10. The method according to any one of claims 1 to 4, wherein the lactobacillus is cultured in an expanded medium according to the following formula in step (1): 60g/L glucose, 2.0g/L urea, KH₂PO₄0.6g/L, 30g/L ammonium chloride, MgSO₄7H₂0.5g/L of O, 0.5g/L of yeast extract, 20g/L of liquid paraffin and water as a solvent; the expansion culture method adopts a variable temperature culture mode, and preferably comprises the following steps: inoculating the seed liquid into a fermentation amplification culture medium according to the volume percentage of 10%, performing fermentation culture for 48 hours at the temperature of 33 ℃ and at the speed of 200r/min, then raising the temperature to 35 ℃, and continuing to culture for 48 hours, wherein the thallus density is more than or equal to 30g wet thallus/L; after the expanded culture, filtering and recovering thalli, and preserving at a low temperature of 4 ℃ for later use; when used, a lactic acid bacterium suspension was prepared with 10mmol/L phosphate buffer solution (pH 8.0).