CN106036325A - Fructus lycii enzyme and preparation method thereof - Google Patents
Fructus lycii enzyme and preparation method thereof Download PDFInfo
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- CN106036325A CN106036325A CN201610454187.1A CN201610454187A CN106036325A CN 106036325 A CN106036325 A CN 106036325A CN 201610454187 A CN201610454187 A CN 201610454187A CN 106036325 A CN106036325 A CN 106036325A
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
- A23L2/382—Other non-alcoholic beverages fermented
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention provides fructus lycii enzyme and a preparation method thereof. The fructus lycii enzyme is prepared from the following raw materials: 40-50wt% of fructus lycii, 4-6wt% of marine fish skin collagen peptide powder, 10-20wt% of liquorice root, 10-15wt% of hericium erinaceus, 5-10wt% of ganoderma lucidum spore powder, 5-10wt% of fructus crataegi and 2-6wt% of a blending agent. The preparation method comprises the steps of grinding the fructus lycii, and then carrying out enzymolysis on the ground fructus lycii by using cellulase to obtain fructus lycii enzymatic hydrolysate; after that, successively carrying out lactic acid fermentation and yeast fermentation on the marine fish skin collagen peptide powder, the hericium erinaceus, the ganoderma lucidum spore powder, the fructus crataegi and the fructus lycii enzymatic hydrolysate obtained in the step (2), homogenizing the obtained fermentation liquor, concentrating, centrifuging and filtering; adding the blending agent into filtrate, filtering and sterilizing. The fructus lycii enzyme not only is capable of improving the appetite of a user, but also has a function of enhancing immunity.
Description
Technical field
The invention belongs to ferment technical field, be specifically related to a kind of Fructus Lycii ferment and preparation method thereof.
Background technology
Ferment is a kind of to be had special bioactive material, the namely " metabolism of yeast secretary by what aminoacid formed
Product ", also referred to as " enzyme ", it is a kind of biocatalyzer, is made up of protein.It is present in the animal and plant body of all work, is
Maintain body normal function, digest food, the required material of the one of the vital movements such as repair tissue.The eighties in 20th century, people
Utilize modern biotechnology to extract the ferment to skin beneficiating to add in cosmetics, to strengthen the function of skin, improve skin
Situation.One of principle of ferment beautifying skin is, by the aged cells digest and decompose in skin, to accelerate it and came off normally
Journey, cleaning skin, remove oils and fats, eliminate comedo, desalinate wrinkle, thoroughly improve skin condition so that it is consolidation, careful, have light
Pool.
Fructus Lycii be health ministry announce be medicine and the article of food, the Pharmacopoeia of the People's Republic of China is bright
Really regulation: " medicinal Fructus Lycii is the dry mature fruit of lycium barbarum ", Zhongning County is the main product ground of lycium barbarum, the Ye Shi world
The place of origin of Fructus Lycii and authentic original producton location.By the end of 2011, more than the 10000 of State Food and Drug Administration's examination and approval
In health product, what prescription used Fructus Lycii has more than 1800 frequency, and shelter has the first place of medicine food raw material.This be enough to Chinese wolfberry health-care is described
Function powerful, has been that the whole nation is generally acknowledged, and that can not exaggerate is referred to as " king of National Health Foods " by Fructus Lycii.Fructus Lycii is strong
Health care greatly and widely so that it is become one of optimum feed stock of producing all kinds of health care product, therefore, produce health food
The most just become the developing direction of Fructus Lycii deep processing.
But, ferment is applied to for Fructus Lycii, in the change of fermentation mode, is primarily present following a few point defect: 1) natural
Fermentation period length, cost are high, fermentation pollution risk is big；2) fermentation mode uses the fermentation of traditional natural flora, modern times compounding purebred
Fermentation or the combination of the two, excessively tradition, without innovation；3) ferment biological Activity and stabill is poor, and the shelf-life is short；4) mouthfeel and wind
Taste bad adaptability, appetite are low；5) ferment with extracting solution, have lost the nutritional labeling of raw material.
Summary of the invention
For solving the problem of existing existence, the present invention proposes a kind of Fructus Lycii ferment, and this ferment can not only improve user
Appetite, but also there is enhancing immunity, to colorectal cancer early stage patient, there is certain curative effect.
The technical scheme is that and be achieved in that:
A kind of Fructus Lycii ferment, calculates according to percetage by weight, makes including following raw material: Fructus Lycii 40～50%, marine fish
Collagen Gly-His-Lys 4～6%, Radix Glycyrrhizae 10～20%, Hericium erinaceus (Bull. Ex Fr.) Pers. 10～15%, Ganoderma spore powder 5～10%, Fructus Crataegi 5～10% with
Blender 2～6%.
Further, described blender includes sucrose, oligomeric galactose and acesulfame potassium.
Further, described marine fish collagen Gly-His-Lys is the marine fish collagen Gly-His-Lys that mean molecule quantity is less than 1000.
The marine fish collagen Gly-His-Lys of the present invention is known product, and its domestic commercially available prod is referred to as the oligomeric Gly-His-Lys of marine fish,
The oligomeric Gly-His-Lys of this marine fish has national standard, GB/T 22729-2008 " the oligomeric Gly-His-Lys of marine fish ".
It is a further object to provide the preparation method of a kind of Fructus Lycii ferment, comprise the following steps:
1) calculate according to percetage by weight, weigh Fructus Lycii 40～50%, marine fish collagen Gly-His-Lys 4～6%, Radix Glycyrrhizae 10～
20%, Hericium erinaceus (Bull. Ex Fr.) Pers. 10～15%, Ganoderma spore powder 5～10%, Fructus Crataegi 5～10% are standby with blender 2～6%；
2) Fructus Lycii is pulverized, then carry out enzymolysis processing with cellulase, obtain Fructus Lycii enzymolysis solution；
3) by marine fish collagen Gly-His-Lys, Hericium erinaceus (Bull. Ex Fr.) Pers., Ganoderma spore powder, Fructus Crataegi and step 2) the Fructus Lycii enzymolysis solution that obtains
Carry out high temperature sterilize after mixing and be cooled to room temperature, then adding lactic acid starter strain, lucifuge at a temperature of 30～35 DEG C
Standing for fermentation, until the pH value of this product reaches 3.9～4.5 termination fermentations；
4) to step 3) fermentation liquid that obtains adds Radix Glycyrrhizae, high temperature sterilize also cools down, then add in fermentation liquid 0.1～
The dry yeast of 0.16w/v%, ferments at a temperature of 25～30 DEG C, until the pH value of this product reaches 4.1～4.2 termination fermentations；
5) by step 4) the fermentation liquid homogenizing that obtains, concentrate, centrifugal, filter, filtrate is added blender, then carried out
Filter, sterilizing.
Further, described step 2) hydrolysis temperature is 40～50 DEG C, enzyme dosage is 1500～2000U/g.
Further, described step 5) processing condition is: fermentation liquid is homogenizing 10～5min under 40MPa.
Further, described step 5) concentration condition is: temperature 60～80 DEG C, is concentrated into 15～20Brix.
Beneficial effects of the present invention:
1, marine fish collagen peptide is because of digestibility height, therefore is mainly used in food nutrient fortifying, aminoacid supplement balance
Agent.Owing to marine fish collagen peptide contains abundant nutritional labeling, and there is exclusive humectant properties, make skin be full of profit, light
Clean, high resilience.Can stop outward the sunlight injury to skin, interior prevent the free radical destruction to skin.With marine fish hide glue
Former peptide is as one of supplementary nitrogen source in Fructus Lycii ferment sweat, it is possible to promote well lactic acid bacteria and saccharomycetic growth and
Metabolic process.
2, Radix Glycyrrhizae is a kind of tonification Chinese herbal medicine.A kind of medicine good to human body, medicinal part is root and rhizome, Medicinal Materials Characters
Root is cylinder.Feeble QI, taste is sweet and special.Function cures mainly heat-clearing and toxic substances removing, expelling phlegm for arresting cough, gastral cavity abdomen etc..
3, Fructus Lycii contains abundant carotene, cryptoxanthin, physalin, retinol1, calcium, the ferrum such as B1, B2, C
Etc. the required nutrition of healthy eye, therefore it is good at improving eyesight, so being commonly called as " bright eye ".Anthocyanidin in associating Fructus Lycii, phylloxanthin, cattle
Sulfonic acid, it is possible to play good improving eyesight function.During the fermentation, due to microorganism, to promote that raw material metabolism produces a large amount of for probiotic bacteria
Primary, secondary metabolite such as aminoacid, vitamin, organic acid, various enzyme, aromatic compound, sugar alcohol, polyphenol, polysaccharide,
Outside the organic active ingredients such as small-molecular peptides and substantial amounts of oligosaccharide, but also produce substantial amounts of probiotic cell, superoxides
Dismutase (SOD), eicosapentaenoic acid (EPA), docosahexenoic acid (DHA) and flavone compound etc. have guarantor to the mankind
The many valuable factor of strong effect.Fructus Lycii ferment produces the little molecular functions such as substantial amounts of lactein and becomes at lactic acid bacteria fermentation process
Dividing a large amount of nutrition contained with raw material self and functional component, these beneficiating ingredients greatly facilitate the increasing of intestinal beneficial flora
Value, suppresses harmful bacteria, thus reduces the generation of corrupt substance and noxious substance, playing functional while, also act as regulation intestinal
Road colony balance, strengthens immunity, promotes beauty treatment, hypnotic, the effect of slow down aging.
4, process through cellulase degradation after Fructus Lycii of the present invention is pulverized, do for follow-up lactic acid bacteria fermentation and culture propagation
Good place mat so that in Fructus Lycii, esters separates with pulp, in free state, beneficially human body preferably absorbs, improving eyesight function
More preferably.
5, show through clinical trial, use this Fructus Lycii enzyme nutrient solution, repairing and treating colorectal cancer, short treating period, prevention and
Curative effect is notable, is difficult to recurrence.
Detailed description of the invention
Embodiment 1
A kind of Fructus Lycii ferment, makes including following raw material: Fructus Lycii 40g, marine fish collagen Gly-His-Lys 4g, Radix Glycyrrhizae 16g, hedgehog hydnum
Mushroom 15g, Ganoderma spore powder 10g, Fructus Crataegi 10g and blender 5g, described blender is 2g sucrose, 2g oligomeric galactose and 1g peace match
Honey.
The preparation method of a kind of Fructus Lycii ferment, comprises the following steps:
1) being pulverized by 40g Fructus Lycii, then carry out enzymolysis processing with cellulase, hydrolysis temperature is 40 DEG C, enzyme dosage
It is 1500U/g, obtains Fructus Lycii enzymolysis solution.
2) by 4g marine fish collagen Gly-His-Lys, 15g Hericium erinaceus (Bull. Ex Fr.) Pers., 10g Ganoderma spore powder, 10g Fructus Crataegi and step 2) obtain
Fructus Lycii enzymolysis solution mixing after carry out high temperature sterilize and be cooled to room temperature, then add lactic acid starter strain, the temperature of 30 DEG C
The lower lucifuge standing for fermentation of degree, until the pH value of this product reaches 3.9 termination fermentations；
3) to step 2) fermentation liquid that obtains adds 16g Radix Glycyrrhizae, high temperature sterilize also cools down, then adds in fermentation liquid
The dry yeast of 0.1w/v%, ferments at a temperature of 30 DEG C, until the pH value of this product reaches 4.2 termination fermentations；
4) by step 5) fermentation liquid homogenizing 10min under 40MPa of obtaining；Temperature 60 C, is concentrated into 15Brix；It is centrifugal,
Filter, filtrate add 2g sucrose, 2g oligomeric galactose and 1g acesulfame potassium, then carry out filtering, sterilizing.
Embodiment 2
A kind of Fructus Lycii ferment, calculates according to percetage by weight, makes including following raw material: Fructus Lycii 45g, marine fish collagen
Gly-His-Lys 6g, Radix Glycyrrhizae 20g, Hericium erinaceus (Bull. Ex Fr.) Pers. 10g, Ganoderma spore powder 8g, Fructus Crataegi 9g and blender 2g, described blender be 0.5g sucrose,
0.5g oligomeric galactose and 1g acesulfame potassium.
The preparation method of a kind of Fructus Lycii ferment, comprises the following steps:
1) being pulverized by 45g Fructus Lycii, then carry out enzymolysis processing with cellulase, hydrolysis temperature is 50 DEG C, enzyme dosage
It is 1700U/g, obtains Fructus Lycii enzymolysis solution.
2) by 6g marine fish collagen Gly-His-Lys, 10g Hericium erinaceus (Bull. Ex Fr.) Pers., 8g Ganoderma spore powder, 9g Fructus Crataegi and step 2) obtain
Carry out high temperature sterilize and be cooled to room temperature after the mixing of Fructus Lycii enzymolysis solution, then add lactic acid starter strain, the temperature of 32 DEG C
Lower lucifuge standing for fermentation, until the pH value of this product reaches 4.2 termination fermentations；
3) to step 2) fermentation liquid that obtains adds 20g Radix Glycyrrhizae, high temperature sterilize also cools down, then adds in fermentation liquid
The dry yeast of 0.1w/v%, ferments at a temperature of 27 DEG C, until the pH value of this product reaches 4.1 termination fermentations；
4) by step 5) fermentation liquid homogenizing 10min under 40MPa of obtaining；Temperature 70 C, is concentrated into 17Brix；It is centrifugal,
Filter, filtrate add 0.5g sucrose, 0.5g oligomeric galactose and 1g acesulfame potassium, then carry out filtering, sterilizing.
Embodiment 3
A kind of Fructus Lycii ferment, calculates according to percetage by weight, makes including following raw material: Fructus Lycii 50g, marine fish collagen
Gly-His-Lys 6g, Radix Glycyrrhizae 15g, Hericium erinaceus (Bull. Ex Fr.) Pers. 10g, Ganoderma spore powder 5g, Fructus Crataegi 10g and blender 4g, described blender is 1g sucrose, 2g
Oligomeric galactose and 1g acesulfame potassium.
The preparation method of a kind of Fructus Lycii ferment, comprises the following steps:
1) being pulverized by 35g Fructus Lycii, then carry out enzymolysis processing with cellulase, hydrolysis temperature is 45 DEG C, enzyme dosage
It is 2000U/g, obtains Fructus Lycii enzymolysis solution.
2) by 6g marine fish collagen Gly-His-Lys, 15g Hericium erinaceus (Bull. Ex Fr.) Pers., 10g Ganoderma spore powder, 10g Fructus Crataegi and step 2) obtain
Fructus Lycii enzymolysis solution mixing after carry out high temperature sterilize and be cooled to room temperature, then add lactic acid starter strain, the temperature of 35 DEG C
The lower lucifuge standing for fermentation of degree, until the pH value of this product reaches 4.5 termination fermentations；
3) to step 2) fermentation liquid that obtains adds 20g Radix Glycyrrhizae, high temperature sterilize also cools down, then adds in fermentation liquid
The dry yeast of 0.1w/v%, ferments at a temperature of 27 DEG C, until the pH value of this product reaches 4.1 termination fermentations；
4) by step 5) fermentation liquid homogenizing 5min under 40MPa of obtaining；Temperature 80 DEG C, is concentrated into 20Brix；Centrifugal, mistake
Filter, adds 1g sucrose, 2g oligomeric galactose and 1g acesulfame potassium in filtrate, then carry out filtering, sterilizing.
Embodiment 4
Clinical trial part:
Data: selecting the total case load being diagnosed as colorectal cancer is 135 examples, and the age is between 18～60 years old, the mean age 35
In year, case is randomly divided into 3 groups, often organizes 45 examples, 3 groups of Genders, age, course of disease no significant differences (P ＞ 0.05).One group
Using the embodiment of the present invention 1 to provide Fructus Lycii ferment, each take 50 milliliters, every day takes 2 times sooner or later；One group uses the present invention to implement
The Fructus Lycii ferment that example 2 provides, each takes 100 milliliters, takes 1 every day；3rd group uses the colorectal cancer medicine being commonly used for treatment,
Medicining mode is taken routinely.Efficacy assessment standard is with reference to " the standard of curative effect evaluation that the double footpath of solid tumor is measured " of WHO.After 90 days
Observing curative effect, the total effective rate of embodiment 1 and embodiment 2 is respectively 75%, 81%, and the total effective rate of matched group is 63%.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all essences in the present invention
Within god and principle, any modification, equivalent substitution and improvement etc. made, should be included within the scope of the present invention.
Claims (7)
1. a Fructus Lycii ferment, it is characterised in that according to percetage by weight calculate, make including following raw material: Fructus Lycii 40～
50%, marine fish collagen Gly-His-Lys 4～6%, Radix Glycyrrhizae 10～20%, Hericium erinaceus (Bull. Ex Fr.) Pers. 10～15%, Ganoderma spore powder 5～10%, mountain
Short, bristly hair or beard 5～10% with blender 2～6%.
Fructus Lycii ferment the most according to claim 1, it is characterised in that described blender include sucrose, oligomeric galactose with
Acesulfame potassium.
Fructus Lycii ferment the most according to claim 1, it is characterised in that described marine fish collagen Gly-His-Lys is mean molecule
The amount marine fish collagen Gly-His-Lys less than 1000.
4. the preparation method of a Fructus Lycii ferment, it is characterised in that comprise the following steps:
1) calculate according to percetage by weight, weigh Fructus Lycii 40～50%, marine fish collagen Gly-His-Lys 4～6%, Radix Glycyrrhizae 10～
20%, Hericium erinaceus (Bull. Ex Fr.) Pers. 10～15%, Ganoderma spore powder 5～10%, Fructus Crataegi 5～10% are standby with blender 2～6%；
2) Fructus Lycii is pulverized, then carry out enzymolysis processing with cellulase, obtain Fructus Lycii enzymolysis solution；
3) by marine fish collagen Gly-His-Lys, Hericium erinaceus (Bull. Ex Fr.) Pers., Ganoderma spore powder, Fructus Crataegi and step 2) mixing of the Fructus Lycii enzymolysis solution that obtains
After carry out high temperature sterilize and be cooled to room temperature, then add lactic acid starter strain, at a temperature of 30～35 DEG C lucifuge stand
Fermentation, until the pH value of this product reaches 3.9～4.5 termination fermentations；
4) to step 3) fermentation liquid that obtains adds Radix Glycyrrhizae, high temperature sterilize also cools down, then add in fermentation liquid 0.1～
The dry yeast of 0.16w/v%, ferments at a temperature of 25～30 DEG C, until the pH value of this product reaches 4.1～4.2 termination fermentations；
5) by step 4) the fermentation liquid homogenizing that obtains, concentrate, centrifugal, filter, filtrate adds blender, then carry out filtering,
Sterilizing.
The preparation method of Fructus Lycii ferment the most according to claim 4, it is characterised in that described step 2) hydrolysis temperature is 40
～50 DEG C, enzyme dosage is 1500～2000U/g.
The preparation method of Fructus Lycii ferment the most according to claim 4, it is characterised in that described step 5) processing condition is:
Fermentation liquid is homogenizing 10～5min under 40MPa.
The preparation method of Fructus Lycii ferment the most according to claim 4, it is characterised in that described step 5) concentration condition is:
Temperature 60～80 DEG C, be concentrated into 15～20Brix.
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| CN107156817A (en) * | 2017-05-31 | 2017-09-15 | 苟春虎 | Matrimony vine breast augmentation ferment |
| CN107772460A (en) * | 2017-11-01 | 2018-03-09 | 佛山市三水区敏俊食品有限公司 | A kind of matrimony vine ferment |
| CN112841485A (en) * | 2021-02-03 | 2021-05-28 | 宁夏红枸杞产业有限公司 | Medlar nutrient solution and preparation method thereof |
| CN113243468A (en) * | 2021-04-27 | 2021-08-13 | 邱绚波 | Medlar concentrated protein oral liquid and preparation method thereof |
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