CN108743500A - Not only oral administration but also can external application beautifying face and moistering lotion compound peptide functional food and preparation method thereof - Google Patents
Not only oral administration but also can external application beautifying face and moistering lotion compound peptide functional food and preparation method thereof Download PDFInfo
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- CN108743500A CN108743500A CN201810601764.4A CN201810601764A CN108743500A CN 108743500 A CN108743500 A CN 108743500A CN 201810601764 A CN201810601764 A CN 201810601764A CN 108743500 A CN108743500 A CN 108743500A
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- 235000013376 functional food Nutrition 0.000 title claims abstract description 40
- 239000006210 lotion Substances 0.000 title claims abstract description 38
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9794—Liliopsida [monocotyledons]
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/185—Vegetable proteins
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/645—Proteins of vegetable origin; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/65—Collagen; Gelatin; Keratin; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
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- A—HUMAN NECESSITIES
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/92—Oral administration
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- Engineering & Computer Science (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
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- Birds (AREA)
- Botany (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Molecular Biology (AREA)
- Dermatology (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses not only oral administration but can external application beautifying face and moistering lotion compound peptide functional food and preparation method thereof, belong to integration of drinking and medicinal herbs field of food.Its purpose is to the not only oral administration that a kind of technique is advanced, drug effect is high is provided but also can external application beautifying face and moistering lotion compound peptide functional food and preparation method thereof.The raw material of compound peptide functional food of the present invention includes according to mass fraction：0.1~3 part of Cordceps militaris small-molecular peptides, 0.1~10 part of lbp peptide, 0.1~6 part of donkey-hide gelatin small-molecular peptides, 0.1~10 part of deep-sea fish collagen peptide, 0.1~10 part of Semen Coicis extract and 0.1~10 part of chicory extract.The present invention is used cooperatively using the ingredient of traditional integration of drinking and medicinal herbs with compound peptide, solve production technology backwardness cause integration of drinking and medicinal herbs ingredient to be taken by human body after, absorb the problem that bad and drug effect, the efficacy of a drug cannot be not fully exerted, human body, which is repaired, from inside of human body is damaged skin and cells of superficial layer, skin surface cytothesis, external application smearing is promoted to have effects that skin-moisturizing, skin, remove wrinkle.
Description
Technical field
The invention belongs to integration of drinking and medicinal herbs field of food, and in particular to not only oral administration but also can external application beautifying face and moistering lotion compound peptide work(
Energy food and preparation method thereof.
Background technology
Loving beauty is part of human nature, and face is nursed and modified using various cosmetics, can only deserve to be called primary
Beauty concept, beautiful appearance can not be kept for a long time.Beautifying face and moistering lotion the best way is to be careful in one's diet in daily life, raw
Rule living, environmental factor, then assist the skin care products for being equipped with beauty class.Though such maintenance product is more in the market, effect unobvious.
Effective product contains steroids, heavy metal etc. mostly, very big to human injury.And donkey-hide gelatin, Isin glue collagen these
Ingredient containing collagen, in the form of macromolecular structure existing for, it is impossible to fully absorb utilization.Develop that a kind of effect is good, nothing
The integration of drinking and medicinal herbs product of side effect improves from inside of human body, reaches beautification appearance, and often the benefits of guarantor's youth is the demand of society.Ah
Glue is moisturized the skin by enriching blood, and is conducive to skin care, the successive dynasties by as women cosmetic good merchantable brand, modern research shows that donkey-hide gelatin
The collagen of human body missing can be supplemented, there is beautifying face and moistering lotion, relieve fatigue, improve inferior health and menopause symptom and prevention
The effect of a variety of diseases.Cordceps militaris rich in Cordyceps sinensis polysaccharide there is moisturizing and bacteriostasis, SOD can remove the freedom generated in vivo
Base, contained 19 kinds of trace elements, are the element spectrals with anti-aging effects, and cordycepin can increase the blood circulation of skin surface
And livid ring around eye can be lightened the stain and eliminate, to play beautifying face and moistering lotion, skin-care effect.The fruit of Chinese wolfberry, containing abundant polysaccharides,
Vitamin, carrotene, flavonoids and selenium element etc., these substances can play anti-oxidant work(after entering body
Effect removes free radical extra in vivo, enables body more young.
Invention content
A kind of that the technical problem to be solved in the present invention is to provide techniques is advanced, drug effect is high not only oral administration but can external application U.S.
Hold beauty treatment compound peptide functional food and preparation method thereof.Specifically, be one kind with donkey-hide gelatin peptide, Cordyceps militaris peptide, deep-sea fish collagen
Protein peptides, lbp peptide, the compound medicinal and edible plant of these small-molecular peptides extract be primary raw material compound peptide functional food
And preparation method thereof.
The present invention relates to a kind of not only oral administration but can external application beautifying face and moistering lotion compound peptide functional food, the compound peptide function
The raw material of food includes：Cordceps militaris small-molecular peptides, lbp peptide, donkey-hide gelatin small-molecular peptides, deep-sea fish collagen peptide, coix seed extraction
Object and chicory extract.
Preferably, the raw material of the compound peptide functional food includes according to mass fraction：0.1~3 part of Cordceps militaris small molecule
Peptide, 0.1~10 part of lbp peptide, 0.1~6 part of donkey-hide gelatin small-molecular peptides, 0.1~10 part of deep-sea fish collagen peptide, 0.1~10 part of heart of a lotus seed
Seed of jog's tears extract and 0.1~10 part of chicory extract.
Preferably, the raw material of the compound peptide functional food includes according to mass fraction：0.5~1.5 part of small point of Cordceps militaris
Sub- peptide, 3~8 parts of lbp peptides, 1~3 part of donkey-hide gelatin small-molecular peptides, 3~8 parts of deep-sea fish collagen peptides, 3~8 parts of coix seed extractions
Object and 3~8 parts of chicory extracts.
Preferably, the Cordceps militaris small-molecular peptides are prepared according to the following steps：Cordceps militaris is laggard in 60 DEG C of dry 12h
Row crushes, and particle size after cracking is 200 mesh, is then soaked, and amount of water is 10~12 times of Cordceps militaris quality, is stirred in 55 DEG C of constant temperature
It mixes 3h to be digested, in 100 DEG C of enzyme deactivation activity 10min, is cooled to 30 DEG C or less progress refrigerated centrifuge separation of solid and liquid, gained liquid
As Cordceps militaris small-molecular peptides stoste, then Cordceps militaris small-molecular peptides stoste is crossed into molecular cut off successively and is 100,000 ultrafiltration membranes and cuts
It is 10,000 ultrafiltration membrane to stay molecular weight, obtains the Cordceps militaris small-molecular peptides after ultrafiltration.
Preferably, the lbp peptide is prepared according to the following steps：The fruit of Chinese wolfberry is soaked into 1h, amount of water is the fruit of Chinese wolfberry
10~12 times of quality are digested in 50 DEG C of constant temperature stirring 3h, in 100 DEG C of enzyme deactivation activity 10min, then carry out coarse filtration, slightly
The filter pore size of filter is 500 mesh, and filtering gained liquid is lbp peptide stoste, and lbp peptide stoste is crossed molecular cut off successively
The ultrafiltration membrane for being 10,000 for 100,000 ultrafiltration membranes and molecular cut off obtains the lbp peptide after ultrafiltration.
Preferably, the donkey-hide gelatin small-molecular peptides are prepared according to the following steps：Colla Corii Asini boiling is dissolved, amount of water be Ah
12~15 times, 60~70 DEG C of temperature control of film quality, Colla Corii Asini are digested after melting in 50 DEG C of constant temperature stirring 3h,
100 DEG C of enzyme deactivation activity 10min, then carry out coarse filtration, and filter pore size is 500 mesh when coarse filtration, and gained liquid is small point of donkey-hide gelatin
Sub- peptide stoste, donkey-hide gelatin small-molecular peptides stoste is crossed successively molecular cut off be 100,000 ultrafiltration membranes and molecular cut off be 10,000 it is super
Filter membrane obtains the donkey-hide gelatin small-molecular peptides after ultrafiltration.
Preferably, the deep-sea fish collagen peptide is prepared according to the following steps：Deep-sea fish skin is soaked into 1h, is added
Water is 10~12 times of deep-sea fish skin quality, is digested in 50 DEG C of constant temperature stirring 3h, in 100 DEG C of enzyme deactivation activity 10min, so
After carry out coarse filtration, filter pore size is 500 mesh when coarse filtration, and gained liquid is deep-sea fish collagen peptide stoste, by deep-sea fish
Collagen peptide stoste crosses the ultrafiltration membrane that molecular cut off is 100,000 ultrafiltration membranes and molecular cut off is 10,000 successively, obtains ultrafiltration
Deep-sea fish collagen peptide afterwards.
The invention further relates to a kind of not only oral administration but can external application beautifying face and moistering lotion compound peptide functional food preparation method, institute
Preparation method is stated to include the following steps：
(1) preparation of Cordceps militaris small-molecular peptides：Cordceps militaris is crushed after 60 DEG C of dry 12h, particle size after cracking 200
Then mesh is soaked, amount of water is 10~12 times of Cordceps militaris quality, is digested in 55 DEG C of constant temperature stirring 3h, at 100 DEG C
Enzyme deactivation activity 10min is cooled to 30 DEG C or less progress refrigerated centrifuge separation of solid and liquid, and gained liquid is Cordceps militaris small molecule peptide former
Liquid, then Cordceps militaris small-molecular peptides stoste is crossed into the ultrafiltration that molecular cut off is 100,000 ultrafiltration membranes and molecular cut off is 10,000 successively
Film obtains the Cordceps militaris small-molecular peptides after ultrafiltration；
(2) preparation of lbp peptide：The fruit of Chinese wolfberry is soaked into 1h, amount of water is 10~12 times of matrimony vine protonatomic mass, 50
DEG C constant temperature stirring 3h is digested, and in 100 DEG C of enzyme deactivation activity 10min, then carries out coarse filtration, the filter pore size of coarse filtration is 500
Mesh, filtering gained liquid is lbp peptide stoste, and it is 100,000 ultrafiltration membranes and retention that lbp peptide stoste is crossed to molecular cut off successively
The ultrafiltration membrane that molecular weight is 10,000, obtains the lbp peptide after ultrafiltration；
(3) preparation of donkey-hide gelatin small-molecular peptides：Colla Corii Asini boiling is dissolved, amount of water is 12~15 times of donkey-hide gelatin tablet quality,
Temperature controls 60~70 DEG C, and Colla Corii Asini is digested after melting in 50 DEG C of constant temperature stirring 3h, in 100 DEG C of enzyme deactivation activity 10min, so
After carry out coarse filtration, filter pore size is 500 mesh when coarse filtration, and gained liquid is donkey-hide gelatin small-molecular peptides stoste, then by small point of donkey-hide gelatin
It is 100,000 ultrafiltration membranes and ultrafiltration membrane that molecular cut off is 10,000 that sub- peptide stoste crosses molecular cut off successively, obtain after ultrafiltration Ah
Glue small-molecular peptides；
(4) preparation of deep-sea fish collagen peptide：Deep-sea fish skin is soaked into 1h, amount of water is deep-sea fish skin quality
It 10~12 times, is digested in 50 DEG C of constant temperature stirring 3h, in 100 DEG C of enzyme deactivation activity 10min, then carries out coarse filtration, mistake when coarse filtration
Filter aperture is 500 mesh, and gained liquid is deep-sea fish collagen peptide stoste, then successively by deep-sea fish collagen peptide stoste
The ultrafiltration membrane that molecular cut off is 100,000 ultrafiltration membranes and molecular cut off is 10,000 is crossed, the deep-sea fish collagen after ultrafiltration is obtained
Peptide；
(5) lbp peptide made from Cordceps militaris small-molecular peptides made from step (1) and step (2) after mixing, is obtained
Small-molecular peptides mixed liquor 1；Deep-sea fish collagen peptide made from donkey-hide gelatin small-molecular peptides made from step (3) and step (4) is mixed
Small-molecular peptides mixed liquor 2 is obtained after closing uniformly；
(6) Semen Coicis extract and chicory extract are added to the water, amount of water is that Semen Coicis extract is extracted with witloof
7~10 times of object gross mass, after stirring and dissolving, it is 300,000 ultrafiltration membranes to cross molecular cut off, obtains extract mixed liquor 3；
(7) small-molecular peptides mixed liquor 1, small-molecular peptides mixed liquor 2 and extract mixed liquor 3 are stirred into 1h in 45 DEG C of constant temperature,
Then high-temperature sterilization is carried out at 100 DEG C, obtain the not only oral administration but also can external application beautifying face and moistering lotion compound peptide functional food.
Preferably, flavoring agent is additionally added when constant temperature stirs in the step (7).
Preferably, the flavoring agent is one or both of pectin, blueberry concentrated juice.
The not only oral administration of the present invention but can external application the compound peptide functional food of beautifying face and moistering lotion and preparation method thereof with it is existing
Technology the difference is that：
1, the present invention not only oral administration but also can the preparation method of beautifying face and moistering lotion compound peptide functional food of external application peptide molecule is given birth to
Object Technology application is in donkey-hide gelatin, Cordyceps militaris, matrimony vine, deep-sea fish collagen, four kinds of traditional integration of drinking and medicinal herbs ingredient process, through enzyme
Donkey-hide gelatin Chinese caterpillar fungus compound peptide combination product is made in solution.
2, method using the present invention prepares the small molecule oligopeptide to be formed, be fully retained four kinds of traditional integration of drinking and medicinal herbs at
Divide the nutritions such as original vitamin, trace element, after human body intake faster than single amino acid or protein adsorption, more
It is easy to be absorbed by organisms, there is prior bioactivity in terms of body metabolism, it is thin that the impaired skin surface of human body can be repaired
Born of the same parents promote skin surface cytothesis, and external application smearing has effects that skin-moisturizing, skin, removes wrinkle, than conventional integration of drinking and medicinal herbs system
Product have higher biological value, prior physiological function.Moreover, because the powerful bearing capacity that small-molecular peptides have, multiple
Coix seed, witloof are closed, both medicinal and edible plant extracts can fully be absorbed by the body, pure natural, full nutrition, reach certainly
It is interior and outer, the effect of beautifying face and moistering lotion.
3, the present invention not only oral administration but also can external application beautifying face and moistering lotion compound peptide functional food with donkey-hide gelatin small-molecular peptides, Cordceps militaris
Small-molecular peptides, Isin glue collagen peptide, lbp peptide, these four are peptides, compound medicinal and edible plant coix seed and chicory extract,
It is for oral administration, from inside of human body repair cell, promote cytothesis effect.
Specific implementation mode
The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention..
Embodiment 1
Not only the oral administration of the present embodiment but can the beautifying face and moistering lotion compound peptide functional food of external application prepared according to the following steps：
(1) preparation of Cordceps militaris small-molecular peptides：Cordceps militaris is crushed after 60 DEG C of dry 12h, particle size after cracking 200
Then mesh is soaked, amount of water is 10 times of Cordceps militaris quality, is digested in 55 DEG C of constant temperature stirring 3h, in 100 DEG C of enzyme deactivations
Active 10min is cooled to 30 DEG C or less progress refrigerated centrifuge separation of solid and liquid, and gained liquid is Cordceps militaris small-molecular peptides stoste,
Cordceps militaris small-molecular peptides stoste is crossed into the ultrafiltration membrane that molecular cut off is 100,000 ultrafiltration membranes and molecular cut off is 10,000 successively again,
Obtain the Cordceps militaris small-molecular peptides after ultrafiltration；
(2) preparation of lbp peptide：The fruit of Chinese wolfberry is soaked into 1h, amount of water is 12 times of matrimony vine protonatomic mass, in 50 DEG C of perseverances
Temperature stirring 3h is digested, and in 100 DEG C of enzyme deactivation activity 10min, then carries out coarse filtration, the filter pore size of coarse filtration is 500 mesh, mistake
Filter gained liquid is lbp peptide stoste, then it is 100,000 ultrafiltration membranes and retention molecule that lbp peptide stoste is crossed molecular cut off successively
Amount is 10,000 ultrafiltration membrane, obtains the lbp peptide after ultrafiltration；
(3) preparation of donkey-hide gelatin small-molecular peptides：Colla Corii Asini boiling is dissolved, amount of water is 13 times, temperature of donkey-hide gelatin tablet quality
60 DEG C of control, Colla Corii Asini are digested in 50 DEG C of constant temperature stirring 3h after melting, in 100 DEG C of enzyme deactivation activity 10min, are then carried out thick
Filter, filter pore size is 500 mesh when coarse filtration, and gained liquid is donkey-hide gelatin small-molecular peptides stoste, by donkey-hide gelatin small-molecular peptides stoste according to
It is secondary to cross the ultrafiltration membrane that molecular cut off is 100,000 ultrafiltration membranes and molecular cut off is 10,000, obtain the donkey-hide gelatin small-molecular peptides after ultrafiltration；
(4) preparation of deep-sea fish collagen peptide：Deep-sea fish skin is soaked into 1h, amount of water is deep-sea fish skin quality
It 12 times, is digested in 50 DEG C of constant temperature stirring 3h, in 100 DEG C of enzyme deactivation activity 10min, then carries out coarse filtration, filter when coarse filtration
Aperture is 500 mesh, and gained liquid is deep-sea fish collagen peptide stoste, then deep-sea fish collagen peptide stoste is crossed successively and is cut
The ultrafiltration membrane that molecular weight is 100,000 ultrafiltration membranes and molecular cut off is 10,000 is stayed, the deep-sea fish collagen peptide after ultrafiltration is obtained；
(5) lbp peptide made from Cordceps militaris small-molecular peptides made from step (1) and step (2) after mixing, is obtained
Small-molecular peptides mixed liquor 1；Deep-sea fish collagen peptide made from donkey-hide gelatin small-molecular peptides made from step (3) and step (4) is mixed
Small-molecular peptides mixed liquor 2 is obtained after closing uniformly；
(6) Semen Coicis extract and chicory extract are added to the water, amount of water is that Semen Coicis extract is extracted with witloof
8 times of object gross mass, after stirring and dissolving, it is 300,000 ultrafiltration membranes to cross molecular cut off, obtains extract mixed liquor 3；
(7) small-molecular peptides mixed liquor 1, small-molecular peptides mixed liquor 2 and extract mixed liquor 3 are stirred into 1h in 45 DEG C of constant temperature,
Then high-temperature sterilization is carried out at 100 DEG C, obtain the not only oral administration but also can external application beautifying face and moistering lotion compound peptide functional food.
The dosage of each raw material is as follows during this implementation comes：0.1kg Cordceps militaris small-molecular peptides, 10kg lbp peptides, 0.1kg donkey-hide gelatin are small
Molecular peptide, 10kg deep-sea fish collagens peptide, 3kg Semen Coicis extracts and 5kg chicory extracts.
Embodiment 2
Not only the oral administration of the present embodiment but can the beautifying face and moistering lotion compound peptide functional food of external application prepared according to the following steps：
(1) preparation of Cordceps militaris small-molecular peptides：Cordceps militaris is crushed after 60 DEG C of dry 12h, particle size after cracking 200
Then mesh is soaked, amount of water is 12 times of Cordceps militaris quality, is digested in 55 DEG C of constant temperature stirring 3h, in 100 DEG C of enzyme deactivations
Active 10min is cooled to 30 DEG C or less progress refrigerated centrifuge separation of solid and liquid, and gained liquid is Cordceps militaris small-molecular peptides stoste,
Cordceps militaris small-molecular peptides stoste is crossed into the ultrafiltration membrane that molecular cut off is 100,000 ultrafiltration membranes and molecular cut off is 10,000 successively again,
Obtain the Cordceps militaris small-molecular peptides after ultrafiltration；
(2) preparation of lbp peptide：The fruit of Chinese wolfberry is soaked into 1h, amount of water is 10 times of matrimony vine protonatomic mass, in 50 DEG C of perseverances
Temperature stirring 3h is digested, and in 100 DEG C of enzyme deactivation activity 10min, then carries out coarse filtration, the filter pore size of coarse filtration is 500 mesh, mistake
Filter gained liquid is lbp peptide stoste, then it is 100,000 ultrafiltration membranes and retention molecule that lbp peptide stoste is crossed molecular cut off successively
Amount is 10,000 ultrafiltration membrane, obtains the lbp peptide after ultrafiltration；
(3) preparation of donkey-hide gelatin small-molecular peptides：Colla Corii Asini boiling is dissolved, amount of water is 15 times, temperature of donkey-hide gelatin tablet quality
65 DEG C of control, Colla Corii Asini are digested in 50 DEG C of constant temperature stirring 3h after melting, in 100 DEG C of enzyme deactivation activity 10min, are then carried out thick
Filter, filter pore size is 500 mesh when coarse filtration, and gained liquid is donkey-hide gelatin small-molecular peptides stoste, by donkey-hide gelatin small-molecular peptides stoste according to
It is secondary to cross the ultrafiltration membrane that molecular cut off is 100,000 ultrafiltration membranes and molecular cut off is 10,000, obtain the donkey-hide gelatin small-molecular peptides after ultrafiltration；
(4) preparation of deep-sea fish collagen peptide：Deep-sea fish skin is soaked into 1h, amount of water is deep-sea fish skin quality
It 10 times, is digested in 50 DEG C of constant temperature stirring 3h, in 100 DEG C of enzyme deactivation activity 10min, then carries out coarse filtration, filter when coarse filtration
Aperture is 500 mesh, and gained liquid is deep-sea fish collagen peptide stoste, then deep-sea fish collagen peptide stoste is crossed successively and is cut
The ultrafiltration membrane that molecular weight is 100,000 ultrafiltration membranes and molecular cut off is 10,000 is stayed, the deep-sea fish collagen peptide after ultrafiltration is obtained；
(5) lbp peptide made from Cordceps militaris small-molecular peptides made from step (1) and step (2) after mixing, is obtained
Small-molecular peptides mixed liquor 1；Deep-sea fish collagen peptide made from donkey-hide gelatin small-molecular peptides made from step (3) and step (4) is mixed
Small-molecular peptides mixed liquor 2 is obtained after closing uniformly；
(6) Semen Coicis extract and chicory extract are added to the water, amount of water is that Semen Coicis extract is extracted with witloof
9 times of object gross mass, after stirring and dissolving, it is 300,000 ultrafiltration membranes to cross molecular cut off, obtains extract mixed liquor 3；
(7) small-molecular peptides mixed liquor 1, small-molecular peptides mixed liquor 2 and extract mixed liquor 3 are stirred into 1h in 45 DEG C of constant temperature,
Then high-temperature sterilization is carried out at 100 DEG C, obtain the not only oral administration but also can external application beautifying face and moistering lotion compound peptide functional food.
The dosage of each raw material is as follows during this implementation comes：1.5kg Cordceps militaris small-molecular peptides, 5kg lbp peptides, small point of 6kg donkey-hide gelatin
Sub- peptide, 8kg deep-sea fish collagens peptide, 5kg Semen Coicis extracts and 0.1kg chicory extracts.
Embodiment 3
Not only the oral administration of the present embodiment but can the beautifying face and moistering lotion compound peptide functional food of external application prepared according to the following steps：
(1) preparation of Cordceps militaris small-molecular peptides：Cordceps militaris is crushed after 60 DEG C of dry 12h, particle size after cracking 200
Then mesh is soaked, amount of water is 11 times of Cordceps militaris quality, is digested in 55 DEG C of constant temperature stirring 3h, in 100 DEG C of enzyme deactivations
Active 10min is cooled to 30 DEG C or less progress refrigerated centrifuge separation of solid and liquid, and gained liquid is Cordceps militaris small-molecular peptides stoste,
Cordceps militaris small-molecular peptides stoste is crossed into the ultrafiltration membrane that molecular cut off is 100,000 ultrafiltration membranes and molecular cut off is 10,000 successively again,
Obtain the Cordceps militaris small-molecular peptides after ultrafiltration；
(2) preparation of lbp peptide：The fruit of Chinese wolfberry is soaked into 1h, amount of water is 11 times of matrimony vine protonatomic mass, in 50 DEG C of perseverances
Temperature stirring 3h is digested, and in 100 DEG C of enzyme deactivation activity 10min, then carries out coarse filtration, the filter pore size of coarse filtration is 500 mesh, mistake
Filter gained liquid is lbp peptide stoste, then it is 100,000 ultrafiltration membranes and retention molecule that lbp peptide stoste is crossed molecular cut off successively
Amount is 10,000 ultrafiltration membrane, obtains the lbp peptide after ultrafiltration；
(3) preparation of donkey-hide gelatin small-molecular peptides：Colla Corii Asini boiling is dissolved, amount of water is 12 times, temperature of donkey-hide gelatin tablet quality
70 DEG C of control, Colla Corii Asini are digested in 50 DEG C of constant temperature stirring 3h after melting, in 100 DEG C of enzyme deactivation activity 10min, are then carried out thick
Filter, filter pore size is 500 mesh when coarse filtration, and gained liquid is donkey-hide gelatin small-molecular peptides stoste, by donkey-hide gelatin small-molecular peptides stoste according to
It is secondary to cross the ultrafiltration membrane that molecular cut off is 100,000 ultrafiltration membranes and molecular cut off is 10,000, obtain the donkey-hide gelatin small-molecular peptides after ultrafiltration；
(4) preparation of deep-sea fish collagen peptide：Deep-sea fish skin is soaked into 1h, amount of water is deep-sea fish skin quality
It 12 times, is digested in 50 DEG C of constant temperature stirring 3h, in 100 DEG C of enzyme deactivation activity 10min, then carries out coarse filtration, filter when coarse filtration
Aperture is 500 mesh, and gained liquid is deep-sea fish collagen peptide stoste, then deep-sea fish collagen peptide stoste is crossed successively and is cut
The ultrafiltration membrane that molecular weight is 100,000 ultrafiltration membranes and molecular cut off is 10,000 is stayed, the deep-sea fish collagen peptide after ultrafiltration is obtained；
(5) lbp peptide made from Cordceps militaris small-molecular peptides made from step (1) and step (2) after mixing, is obtained
Small-molecular peptides mixed liquor 1；Deep-sea fish collagen peptide made from donkey-hide gelatin small-molecular peptides made from step (3) and step (4) is mixed
Small-molecular peptides mixed liquor 2 is obtained after closing uniformly；
(6) Semen Coicis extract and chicory extract are added to the water, amount of water is that Semen Coicis extract is extracted with witloof
8 times of object gross mass, after stirring and dissolving, it is 300,000 ultrafiltration membranes to cross molecular cut off, obtains extract mixed liquor 3；
(7) small-molecular peptides mixed liquor 1, small-molecular peptides mixed liquor 2 and extract mixed liquor 3 are stirred into 1h in 45 DEG C of constant temperature,
Pectin is added when stirring so that amount of the pectin content in output aggregate is 0.08g/kg, and high-temperature sterilization is then carried out at 100 DEG C,
Obtain the not only oral administration but can external application beautifying face and moistering lotion compound peptide functional food.
The dosage of each raw material is as follows during this implementation comes：3kg Cordceps militaris small-molecular peptides, 3kg lbp peptides, 1kg donkey-hide gelatin small molecules
Peptide, 5kg deep-sea fish collagens peptide, 0.1kg Semen Coicis extracts and 8kg chicory extracts.
Embodiment 4
Not only the oral administration of the present embodiment but can the beautifying face and moistering lotion compound peptide functional food of external application prepared according to the following steps：
(1) preparation of Cordceps militaris small-molecular peptides：Cordceps militaris is crushed after 60 DEG C of dry 12h, particle size after cracking 200
Then mesh is soaked, amount of water is 10 times of Cordceps militaris quality, is digested in 55 DEG C of constant temperature stirring 3h, in 100 DEG C of enzyme deactivations
Active 10min is cooled to 30 DEG C or less progress refrigerated centrifuge separation of solid and liquid, and gained liquid is Cordceps militaris small-molecular peptides stoste,
Cordceps militaris small-molecular peptides stoste is crossed into the ultrafiltration membrane that molecular cut off is 100,000 ultrafiltration membranes and molecular cut off is 10,000 successively again,
Obtain the Cordceps militaris small-molecular peptides after ultrafiltration；
(2) preparation of lbp peptide：The fruit of Chinese wolfberry is soaked into 1h, amount of water is 12 times of matrimony vine protonatomic mass, in 50 DEG C of perseverances
Temperature stirring 3h is digested, and in 100 DEG C of enzyme deactivation activity 10min, then carries out coarse filtration, the filter pore size of coarse filtration is 500 mesh, mistake
Filter gained liquid is lbp peptide stoste, then it is 100,000 ultrafiltration membranes and retention molecule that lbp peptide stoste is crossed molecular cut off successively
Amount is 10,000 ultrafiltration membrane, obtains the lbp peptide after ultrafiltration；
(3) preparation of donkey-hide gelatin small-molecular peptides：Colla Corii Asini boiling is dissolved, amount of water is 14 times, temperature of donkey-hide gelatin tablet quality
68 DEG C of control, Colla Corii Asini are digested in 50 DEG C of constant temperature stirring 3h after melting, in 100 DEG C of enzyme deactivation activity 10min, are then carried out thick
Filter, filter pore size is 500 mesh when coarse filtration, and gained liquid is donkey-hide gelatin small-molecular peptides stoste, by donkey-hide gelatin small-molecular peptides stoste according to
It is secondary to cross the ultrafiltration membrane that molecular cut off is 100,000 ultrafiltration membranes and molecular cut off is 10,000, obtain the donkey-hide gelatin small-molecular peptides after ultrafiltration；
(4) preparation of deep-sea fish collagen peptide：Deep-sea fish skin is soaked into 1h, amount of water is deep-sea fish skin quality
It 11 times, is digested in 50 DEG C of constant temperature stirring 3h, in 100 DEG C of enzyme deactivation activity 10min, then carries out coarse filtration, filter when coarse filtration
Aperture is 500 mesh, and gained liquid is deep-sea fish collagen peptide stoste, then deep-sea fish collagen peptide stoste is crossed successively and is cut
The ultrafiltration membrane that molecular weight is 100,000 ultrafiltration membranes and molecular cut off is 10,000 is stayed, the deep-sea fish collagen peptide after ultrafiltration is obtained；
(5) lbp peptide made from Cordceps militaris small-molecular peptides made from step (1) and step (2) after mixing, is obtained
Small-molecular peptides mixed liquor 1；Deep-sea fish collagen peptide made from donkey-hide gelatin small-molecular peptides made from step (3) and step (4) is mixed
Small-molecular peptides mixed liquor 2 is obtained after closing uniformly；
(6) Semen Coicis extract and chicory extract are added to the water, amount of water is that Semen Coicis extract is extracted with witloof
10 times of object gross mass, after stirring and dissolving, it is 300,000 ultrafiltration membranes to cross molecular cut off, obtains extract mixed liquor 3；
(7) small-molecular peptides mixed liquor 1, small-molecular peptides mixed liquor 2 and extract mixed liquor 3 are stirred into 1h in 45 DEG C of constant temperature,
Then high-temperature sterilization is carried out at 100 DEG C, obtain the not only oral administration but also can external application beautifying face and moistering lotion compound peptide functional food.
The dosage of each raw material is as follows during this implementation comes：0.5kg Cordceps militaris small-molecular peptides, 3kg lbp peptides, small point of 2kg donkey-hide gelatin
Sub- peptide, 3kg deep-sea fish collagens peptide, 10kg Semen Coicis extracts and 3kg chicory extracts.
Embodiment 5
Not only the oral administration of the present embodiment but can the beautifying face and moistering lotion compound peptide functional food of external application prepared according to the following steps：
(1) preparation of Cordceps militaris small-molecular peptides：Cordceps militaris is crushed after 60 DEG C of dry 12h, particle size after cracking 200
Then mesh is soaked, amount of water is 12 times of Cordceps militaris quality, is digested in 55 DEG C of constant temperature stirring 3h, in 100 DEG C of enzyme deactivations
Active 10min is cooled to 30 DEG C or less progress refrigerated centrifuge separation of solid and liquid, and gained liquid is Cordceps militaris small-molecular peptides stoste,
Cordceps militaris small-molecular peptides stoste is crossed into the ultrafiltration membrane that molecular cut off is 100,000 ultrafiltration membranes and molecular cut off is 10,000 successively again,
Obtain the Cordceps militaris small-molecular peptides after ultrafiltration；
(2) preparation of lbp peptide：The fruit of Chinese wolfberry is soaked into 1h, amount of water is 10 times of matrimony vine protonatomic mass, in 50 DEG C of perseverances
Temperature stirring 3h is digested, and in 100 DEG C of enzyme deactivation activity 10min, then carries out coarse filtration, the filter pore size of coarse filtration is 500 mesh, mistake
Filter gained liquid is lbp peptide stoste, then it is 100,000 ultrafiltration membranes and retention molecule that lbp peptide stoste is crossed molecular cut off successively
Amount is 10,000 ultrafiltration membrane, obtains the lbp peptide after ultrafiltration；
(3) preparation of donkey-hide gelatin small-molecular peptides：Colla Corii Asini boiling is dissolved, amount of water is 15 times, temperature of donkey-hide gelatin tablet quality
63 DEG C of control, Colla Corii Asini are digested in 50 DEG C of constant temperature stirring 3h after melting, in 100 DEG C of enzyme deactivation activity 10min, are then carried out thick
Filter, filter pore size is 500 mesh when coarse filtration, and gained liquid is donkey-hide gelatin small-molecular peptides stoste, by donkey-hide gelatin small-molecular peptides stoste according to
It is secondary to cross the ultrafiltration membrane that molecular cut off is 100,000 ultrafiltration membranes and molecular cut off is 10,000, obtain the donkey-hide gelatin small-molecular peptides after ultrafiltration；
(4) preparation of deep-sea fish collagen peptide：Deep-sea fish skin is soaked into 1h, amount of water is deep-sea fish skin quality
It 10 times, is digested in 50 DEG C of constant temperature stirring 3h, in 100 DEG C of enzyme deactivation activity 10min, then carries out coarse filtration, filter when coarse filtration
Aperture is 500 mesh, and gained liquid is deep-sea fish collagen peptide stoste, then deep-sea fish collagen peptide stoste is crossed successively and is cut
The ultrafiltration membrane that molecular weight is 100,000 ultrafiltration membranes and molecular cut off is 10,000 is stayed, the deep-sea fish collagen peptide after ultrafiltration is obtained；
(5) lbp peptide made from Cordceps militaris small-molecular peptides made from step (1) and step (2) after mixing, is obtained
Small-molecular peptides mixed liquor 1；Deep-sea fish collagen peptide made from donkey-hide gelatin small-molecular peptides made from step (3) and step (4) is mixed
Small-molecular peptides mixed liquor 2 is obtained after closing uniformly；
(6) Semen Coicis extract and chicory extract are added to the water, amount of water is that Semen Coicis extract is extracted with witloof
7 times of object gross mass, after stirring and dissolving, it is 300,000 ultrafiltration membranes to cross molecular cut off, obtains extract mixed liquor 3；
(7) small-molecular peptides mixed liquor 1, small-molecular peptides mixed liquor 2 and extract mixed liquor 3 are stirred into 1h in 45 DEG C of constant temperature,
Blueberry concentrated juice is added when stirring so that amount of the blueberry concentrated juice content in output aggregate is 2g/kg, then at 100 DEG C
Carry out high-temperature sterilization, obtain the not only oral administration but also can external application beautifying face and moistering lotion compound peptide functional food.
The dosage of each raw material is as follows during this implementation comes：1kg Cordceps militaris small-molecular peptides, 0.1kg lbp peptides, small point of 3kg donkey-hide gelatin
Sub- peptide, 0.1kg deep-sea fish collagens peptide, 8kg Semen Coicis extracts and 0.1kg chicory extracts.
The product of the present invention is using the extract of the compound medicinal and edible plant of compound peptide as major ingredient, then takes pectin, blueberry concentration
Fruit juice etc. is cooked auxiliary material, and gained finished product taste is good, has the effect of from-inner-to-outer, beautifying face and moistering lotion.
Note：Extract used in above example, is commercially available.
Verification test
Not only oral administration using the present invention but can external application beautifying face and moistering lotion compound peptide functional food carry out trial population, as a result
Show the compound peptide functional food of the present invention with donkey-hide gelatin small-molecular peptides, Cordceps militaris small-molecular peptides, deep-sea fish collagen peptide, matrimony vine
Peptide, these four are peptides, compound medicinal and edible plant coix seed and chicory extract, are taken orally, from inside of human body repair cell, are promoted
Into cytothesis effect；External application has effects that skin-moisturizing, skin, removes wrinkle, since small-molecular peptides have bearing capacity,
Other effective components can be promoted preferably to be absorbed by the body, pure natural, full nutrition reaches and improves skin from inside to outside, and beauty is supported
The effect of face.
Typical case：
Zhang, female, 48 years old, face was aged, more wrinkles, by dual-purpose inside and outside the present invention, 30 days, and obvious effect.
What so-and-so, female 34 years old, has a haggard face, is pale, dual-purpose inside and outside the present invention is obviously improved for 15 days.
Liu, female, 37 years old, skin was coarse, dry, and external application is smeared, 7 days, hence it is evident that improves.
Liu, female, 26 years old, skin was coarse, more acnes, and external application is smeared, 10 days, obvious effect.
Mr. Li, female, 45 years old, skin sunburn, coarse, external application smearing, 5 days, hence it is evident that repair.
Zheng, female, 57 years old, face was aged, anaemia, took orally, 30 days, hence it is evident that improves.
Mr. Wang, female, 31 years old, after orthopedic, swelling and pain, inside and outside dual-purpose, 3 days, swelling and pain relieving.
Wu so-and-so, man, 51 years old, skin was coarse, aged, inside and outside dual-purpose, 20 days, hence it is evident that improve.
Although above having used general explanation and specific embodiment, the present invention is described in detail, at this
On the basis of invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Therefore,
These modifications or improvements without departing from theon the basis of the spirit of the present invention belong to the scope of protection of present invention.
Claims (10)
1. a kind of not only oral administration but can external application beautifying face and moistering lotion compound peptide functional food, it is characterised in that：The compound peptide function
The raw material of food includes：Cordceps militaris small-molecular peptides, lbp peptide, donkey-hide gelatin small-molecular peptides, deep-sea fish collagen peptide, coix seed extraction
Object and chicory extract.
2. not only oral administration according to claim 1 but can external application beautifying face and moistering lotion compound peptide functional food, feature exists
In：The raw material of the compound peptide functional food includes according to mass fraction：0.1~3 part of Cordceps militaris small-molecular peptides, 0.1~10 part
Lbp peptide, 0.1~6 part of donkey-hide gelatin small-molecular peptides, 0.1~10 part of deep-sea fish collagen peptide, 0.1~10 part of Semen Coicis extract and
0.1~10 part of chicory extract.
3. not only oral administration according to claim 2 but can external application beautifying face and moistering lotion compound peptide functional food, feature exists
In：The raw material of the compound peptide functional food includes according to mass fraction：0.5~1.5 part of Cordceps militaris small-molecular peptides, 3~8 parts of Chinese hollys
Qi peptide, 1~3 part of donkey-hide gelatin small-molecular peptides, 3~8 parts of deep-sea fish collagen peptides, 3~8 parts of Semen Coicis extracts and 3~8 portions of witloofs
Extract.
4. not only oral administration according to claim 1 but can external application beautifying face and moistering lotion compound peptide functional food, it is characterised in that：
The Cordceps militaris small-molecular peptides are prepared according to the following steps：Cordceps militaris is crushed after 60 DEG C of dry 12h, particle size after cracking
For 200 mesh, then to soak, amount of water is 10~12 times of Cordceps militaris quality, is digested in 55 DEG C of constant temperature stirring 3h,
100 DEG C of enzyme deactivation activity 10min are cooled to 30 DEG C or less progress refrigerated centrifuge separation of solid and liquid, and gained liquid is small point of Cordceps militaris
Sub- peptide stoste, by Cordceps militaris small-molecular peptides stoste, molecular cut off is 100,000 ultrafiltration membranes excessively successively and molecular cut off is 10,000
Ultrafiltration membrane obtains the Cordceps militaris small-molecular peptides after ultrafiltration.
5. not only oral administration according to claim 1 but can external application beautifying face and moistering lotion compound peptide functional food, it is characterised in that：
The lbp peptide is prepared according to the following steps：The fruit of Chinese wolfberry is soaked into 1h, amount of water is 10~12 times of matrimony vine protonatomic mass,
It is digested in 50 DEG C of constant temperature stirring 3h, in 100 DEG C of enzyme deactivation activity 10min, then carries out coarse filtration, the filter pore size of coarse filtration is
500 mesh, filtering gained liquid is lbp peptide stoste, and lbp peptide stoste is crossed to molecular cut off successively and is 100,000 ultrafiltration membranes and cuts
It is 10,000 ultrafiltration membrane to stay molecular weight, obtains the lbp peptide after ultrafiltration.
6. not only oral administration according to claim 1 but can external application beautifying face and moistering lotion compound peptide functional food, it is characterised in that：
The donkey-hide gelatin small-molecular peptides are prepared according to the following steps：Colla Corii Asini boiling is dissolved, amount of water be donkey-hide gelatin tablet quality 12~
15 times, 60~70 DEG C of temperature control, Colla Corii Asini is digested after melting in 50 DEG C of constant temperature stirring 3h, in 100 DEG C of enzyme deactivation activity
10min, then carries out coarse filtration, and filter pore size is 500 mesh when coarse filtration, and gained liquid is donkey-hide gelatin small-molecular peptides stoste, by Ah
Glue small-molecular peptides stoste crosses the ultrafiltration membrane that molecular cut off is 100,000 ultrafiltration membranes and molecular cut off is 10,000 successively, obtains ultrafiltration
Donkey-hide gelatin small-molecular peptides afterwards.
7. not only oral administration according to claim 1 but can external application beautifying face and moistering lotion compound peptide functional food, it is characterised in that：
The deep-sea fish collagen peptide is prepared according to the following steps：Deep-sea fish skin is soaked into 1h, amount of water is deep-sea fish skin
10~12 times of quality are digested in 50 DEG C of constant temperature stirring 3h, in 100 DEG C of enzyme deactivation activity 10min, then carry out coarse filtration, slightly
Filter pore size is 500 mesh when filter, and gained liquid is deep-sea fish collagen peptide stoste, by deep-sea fish collagen peptide stoste
The ultrafiltration membrane that molecular cut off is 100,000 ultrafiltration membranes and molecular cut off is 10,000 is crossed successively, obtains the deep-sea fish collagen after ultrafiltration
Protein peptides.
8. a kind of not only oral administration but can external application beautifying face and moistering lotion compound peptide functional food preparation method, it is characterised in that：It is described
Preparation method includes the following steps：
(1) preparation of Cordceps militaris small-molecular peptides：Cordceps militaris is crushed after 60 DEG C of dry 12h, particle size after cracking is 200 mesh,
Then it soaks, amount of water is 10~12 times of Cordceps militaris quality, is digested in 55 DEG C of constant temperature stirring 3h, is gone out at 100 DEG C
Enzymatic activity 10min is cooled to 30 DEG C or less progress refrigerated centrifuge separation of solid and liquid, and gained liquid is Cordceps militaris small molecule peptide former
Cordceps militaris small-molecular peptides stoste is crossed the ultrafiltration membrane that molecular cut off is 100,000 ultrafiltration membranes and molecular cut off is 10,000 by liquid successively,
Obtain the Cordceps militaris small-molecular peptides after ultrafiltration；
(2) preparation of lbp peptide：The fruit of Chinese wolfberry is soaked into 1h, amount of water is 10~12 times of matrimony vine protonatomic mass, in 50 DEG C of perseverances
Temperature stirring 3h is digested, and in 100 DEG C of enzyme deactivation activity 10min, then carries out coarse filtration, the filter pore size of coarse filtration is 500 mesh, mistake
Filter gained liquid is lbp peptide stoste, and it is 100,000 ultrafiltration membranes and molecular cut off that lbp peptide stoste is crossed to molecular cut off successively
For 10,000 ultrafiltration membrane, the lbp peptide after ultrafiltration is obtained；
(3) preparation of donkey-hide gelatin small-molecular peptides：Colla Corii Asini boiling is dissolved, amount of water is 12~15 times, temperature of donkey-hide gelatin tablet quality
60~70 DEG C of control, Colla Corii Asini is digested after melting in 50 DEG C of constant temperature stirring 3h, in 100 DEG C of enzyme deactivation activity 10min, then into
Row coarse filtration, filter pore size is 500 mesh when coarse filtration, and gained liquid is donkey-hide gelatin small-molecular peptides stoste, by donkey-hide gelatin small molecule peptide former
Liquid crosses the ultrafiltration membrane that molecular cut off is 100,000 ultrafiltration membranes and molecular cut off is 10,000 successively, obtains small point of the donkey-hide gelatin after ultrafiltration
Sub- peptide；
(4) preparation of deep-sea fish collagen peptide：Deep-sea fish skin is soaked into 1h, amount of water be deep-sea fish skin quality 10~
It 12 times, is digested in 50 DEG C of constant temperature stirring 3h, in 100 DEG C of enzyme deactivation activity 10min, then carries out coarse filtration, filter when coarse filtration
Aperture is 500 mesh, and gained liquid is deep-sea fish collagen peptide stoste, and deep-sea fish collagen peptide stoste is crossed successively and is retained
The ultrafiltration membrane that molecular weight is 100,000 ultrafiltration membranes and molecular cut off is 10,000 obtains the deep-sea fish collagen peptide after ultrafiltration；
(5) lbp peptide made from Cordceps militaris small-molecular peptides made from step (1) and step (2) after mixing, is obtained small point
Sub- peptide mixed liquor 1；Deep-sea fish collagen peptide made from donkey-hide gelatin small-molecular peptides made from step (3) and step (4) is mixed equal
Small-molecular peptides mixed liquor 2 is obtained after even；
(6) Semen Coicis extract and chicory extract are added to the water, amount of water is that Semen Coicis extract and chicory extract are total
7~10 times of quality, after stirring and dissolving, it is 300,000 ultrafiltration membranes to cross molecular cut off, obtains extract mixed liquor 3；
(7) small-molecular peptides mixed liquor 1, small-molecular peptides mixed liquor 2 and extract mixed liquor 3 are stirred into 1h in 45 DEG C of constant temperature, then
High-temperature sterilization is carried out at 100 DEG C, obtain the not only oral administration but also can external application beautifying face and moistering lotion compound peptide functional food.
9. not only oral administration according to claim 8 but can external application beautifying face and moistering lotion compound peptide functional food preparation method,
It is characterized in that：It is additionally added flavoring agent when constant temperature stirs in the step (7).
10. not only oral administration according to claim 9 but can external application beautifying face and moistering lotion compound peptide functional food preparation method,
It is characterized in that：The flavoring agent is one or both of pectin, blueberry concentrated juice.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111000251A (en) * | 2019-12-26 | 2020-04-14 | 东阿生力源阿胶股份有限公司 | Donkey-hide gelatin peptide composition and application |
| CN111110833A (en) * | 2019-12-23 | 2020-05-08 | 西安大重澳生物科技有限公司 | A skin caring composition for oral administration and topical application, and its preparation method |
| CN112336655A (en) * | 2020-12-14 | 2021-02-09 | 上海应用技术大学 | Natural scalp care agent and preparation method and application thereof |
| CN113598378A (en) * | 2021-08-11 | 2021-11-05 | 红华御康生物科技(江苏)股份有限公司 | Absorption-promoting small-molecule peptide functional food and preparation method thereof |
| CN115337220A (en) * | 2022-08-30 | 2022-11-15 | 广州蝶恋花生物科技有限公司 | Natural cordyceps peptide composition, liposome and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101692908A (en) * | 2009-10-19 | 2010-04-14 | 浙江省医学科学院 | Double-glue pollen food with functions of enriching blood, beautifying face and nourishing skin and preparation method thereof |
| CN103169953A (en) * | 2013-03-20 | 2013-06-26 | 天津大学 | Collagen peptide-colla corii asini oral solution and preparation method thereof |
| CN106963718A (en) * | 2017-04-19 | 2017-07-21 | 北京久禾之美生物科技有限公司 | A kind of cosmetic composition with anti aging effect effect |
-
2018
- 2018-06-12 CN CN201810601764.4A patent/CN108743500A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101692908A (en) * | 2009-10-19 | 2010-04-14 | 浙江省医学科学院 | Double-glue pollen food with functions of enriching blood, beautifying face and nourishing skin and preparation method thereof |
| CN103169953A (en) * | 2013-03-20 | 2013-06-26 | 天津大学 | Collagen peptide-colla corii asini oral solution and preparation method thereof |
| CN106963718A (en) * | 2017-04-19 | 2017-07-21 | 北京久禾之美生物科技有限公司 | A kind of cosmetic composition with anti aging effect effect |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111110833A (en) * | 2019-12-23 | 2020-05-08 | 西安大重澳生物科技有限公司 | A skin caring composition for oral administration and topical application, and its preparation method |
| CN111000251A (en) * | 2019-12-26 | 2020-04-14 | 东阿生力源阿胶股份有限公司 | Donkey-hide gelatin peptide composition and application |
| CN112336655A (en) * | 2020-12-14 | 2021-02-09 | 上海应用技术大学 | Natural scalp care agent and preparation method and application thereof |
| CN113598378A (en) * | 2021-08-11 | 2021-11-05 | 红华御康生物科技(江苏)股份有限公司 | Absorption-promoting small-molecule peptide functional food and preparation method thereof |
| CN115337220A (en) * | 2022-08-30 | 2022-11-15 | 广州蝶恋花生物科技有限公司 | Natural cordyceps peptide composition, liposome and application thereof |
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