CN115211403A - Pig semen cultivation method for improving activity and fertility rate - Google Patents
Pig semen cultivation method for improving activity and fertility rate Download PDFInfo
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- 230000004075 alteration Effects 0.000 description 5
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/14—Pretreatment of feeding-stuffs with enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The invention discloses a pig semen cultivation method for improving activity and fertility rate, which comprises the following steps: mixing a mulberry modified fermentation material, lysine and a linseed extracting solution according to a weight ratio of 1; then mixing the corn, the vitamins and the wheat bran according to a weight ratio of 4; according to the invention, the improved additive is prepared from the mulberry modified fermentation material, lysine and the linseed extract, the modified additive is matched with a bottom material formed by corn, vitamins and wheat bran, the two materials are mixed to be used as a special material for breeding nutrition of the pig semen, the activity and fertility rate of the pig semen can be enhanced by the synergistic effect of the mulberry modified fermentation material and the linseed extract, the quality of the semen is improved, the mulberry fermentation material is matched with the chitosan liquid in the preparation of the mulberry modified fermentation material, the synergistic effect of the linseed extract can be further enhanced, and the activity and fertility rate of the extracted semen are stronger.
Description
Technical Field
The invention relates to the technical field of pig semen cultivation, in particular to a pig semen cultivation method for improving activity and fertility rate.
Background
The pig is vertebrate, mammal, livestock, and ancient omnivorous mammal. It is divided into domestic pig and wild pig. Pigs are now generally considered an acronym for porcine. The physical characteristics of pigs are different according to different breeds. But it is usually characterized by large ears, long head, straight nose, and narrow back and waist. The hair is thick and hard and is divided into white, pink, black, brown and flower color according to different varieties; boar semen is a suspension of boar sperm cells that is secreted by boar reproductive organs, including the accessory glands.
The existing pig semen is firstly required to improve the semen quality in the cultivation process, but the pig semen has low activity and poor fertilization rate, so that the cultivation efficiency is limited.
Disclosure of Invention
In view of the defects of the prior art, the invention aims to provide a pig semen cultivation method for improving activity and fertilization rate so as to solve the problems in the background technology.
The technical scheme adopted by the invention for solving the technical problems is as follows:
the invention provides a pig semen cultivation method for improving activity and fertility rate, which comprises the following steps:
the method comprises the following steps: mixing a mulberry modified fermentation material, lysine and a flaxseed extract according to a weight ratio of 1;
step two: then mixing the corn, the vitamins and the wheat bran according to a weight ratio of 4 to 1 to obtain a base material, and mixing the improved additive and the base material according to a weight ratio of 1 to 6 to prepare the special material for the pig;
step three: feeding pigs at 6 o' clock every day by adopting the special pig feed, feeding the pigs at 2.2Kg every day for 3 months, and collecting semen;
step four: and after semen is collected, heat preservation is carried out in a heat preservation box, and the heat preservation temperature is 20-25 ℃.
Preferably, the preparation method of the mulberry modified fermentation material comprises the following steps:
s01: mashing mulberries into residues, adding cellulase and pectinase, and performing enzymolysis treatment to obtain an enzymolysis mulberry material after the enzymolysis is finished;
s02: carrying out enzymolysis on a mulberry material, inactivating enzyme, then placing in a fermentation tank for fermentation, adding lactobacillus accounting for 5-10% of the total amount of the enzymolysis material and glucose accounting for 5-9% of the total amount of the enzymolysis material, fermenting at the temperature of 35-39 ℃ for 2-3d, and finishing the fermentation to obtain a mulberry fermentation material;
s03: and (3) preparing and blending the mulberry fermented material and the chitosan liquid according to the weight ratio of 5.
The inventor discovers that the special pig feed has excellent sperm motility and sperm aberration rate performance, no chitosan solution is added, no wolfberry polysaccharide extractant is added in the preparation of the chitosan solution, and the mulberry fermented feed adopts the liquorice raw material to replace fermentation, so that the performance of the product is reduced, meanwhile, the linseed extract is not added, the performance of the product is reduced, the linseed extract and the mulberry modified fermented feed have a coordinated effect, the sperm motility and the sperm aberration rate are enhanced together, and the improvement effect is obvious;
the inventor of the invention also finds that only the mulberry modified fermentation material prepared by the method can realize remarkable effect, can remarkably reduce sperm teratogenesis rate and improve activity, and the effect of the invention can not be realized by other methods;
meanwhile, the linseed extract and the mulberry modified fermentation material have a synergistic effect and are matched together, so that the quality of sperms is remarkably enhanced, and the improvement efficiency is remarkable.
Preferably, the adding amount of the cellulase and the pectinase is 5% and 9% of the total amount of the mulberry residues respectively.
Preferably, the temperature of the enzymolysis treatment is 32-35 ℃, and the enzymolysis time is 35-45min.
Preferably, the preparation method of the chitosan solution comprises the following steps:
adding chitosan into 2-3 times of sodium alginate solution, dispersing thoroughly, adding fructus Lycii polysaccharide extractant 1-5% of chitosan, and stirring thoroughly to obtain chitosan solution.
Preferably, the mass fraction of the sodium alginate solution is 10-15%.
Preferably, the preparation method of the lycium barbarum polysaccharide extracting agent comprises the following steps:
the lycium barbarum polysaccharide extracting agent is prepared by the steps of feeding the lycium barbarum into a pounding machine for pounding, adding 5 times of ethanol solvent for ultrasonic treatment, carrying out ultrasonic power of 150-210W, carrying out ultrasonic time of 20-30min, removing alcohol after finishing ultrasonic treatment, boiling and extracting in 4-9 times of water for 2-5h, finishing extraction, and filtering.
Preferably, the preparation method of the linseed extract comprises the following steps: crushing flaxseed, freezing at-5 deg.C for 1-2 hr, thawing in deionized water, adding 10-15 times of ethanol organic solvent, extracting, and recovering ethanol solvent under reduced pressure to obtain flaxseed extractive solution.
Preferably, the dispersed thawing adopts water with the temperature of 20 ℃ to thaw the flaxseeds firstly, the flaxseeds are completely thawed, and then the flaxseeds are stirred and dispersed in the water fully.
Preferably, the extraction treatment adopts 10-20MPa of pressure and the extraction temperature is 55-65 ℃.
Compared with the prior art, the invention has the following beneficial effects:
according to the invention, the improved additive is prepared from the mulberry modified fermentation material, lysine and the linseed extract, the modified additive is matched with a bottom material formed by corn, vitamins and wheat bran, the two materials are mixed to be used as a special material for breeding nutrition of the pig semen, the activity and fertility rate of the pig semen can be enhanced by the synergistic effect of the mulberry modified fermentation material and the linseed extract, the quality of the semen is improved, the mulberry fermentation material is matched with the chitosan liquid in the preparation of the mulberry modified fermentation material, the synergistic effect of the linseed extract can be further enhanced, and the activity and fertility rate of the extracted semen are stronger.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below with reference to specific embodiments, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The pig semen cultivation method for improving activity and fertility rate comprises the following steps:
the method comprises the following steps: mixing a mulberry modified fermentation material, lysine and a flaxseed extract according to a weight ratio of 1;
step two: then mixing the corn, the vitamins and the wheat bran according to a weight ratio of 4 to 1 to obtain a base material, and mixing the improved additive and the base material according to a weight ratio of 1 to 6 to prepare the special material for the pig;
step three: feeding pigs at 6 o' clock every day by adopting the special pig feed, feeding the pigs at 2.2Kg every day for 3 months, and collecting semen;
step four: and after semen is collected, heat preservation is carried out in a heat preservation box, and the heat preservation temperature is 20-25 ℃.
The preparation method of the mulberry modified fermentation material comprises the following steps:
s01: crushing mulberries into residues, adding cellulase and pectinase, and performing enzymolysis to obtain an enzymolysis mulberry material after the enzymolysis is finished;
s02: carrying out enzymolysis on mulberry materials, deactivating enzyme, then placing the mulberry materials in a fermentation tank for fermentation, adding lactobacillus accounting for 5-10% of the total amount of the enzymolysis materials and glucose accounting for 5-9% of the total amount of the enzymolysis materials, wherein the fermentation temperature is 35-39 ℃, the fermentation time is 2-3d, and after the fermentation is finished, obtaining mulberry fermentation materials;
s03: and (3) preparing and blending the mulberry fermented material and the chitosan liquid according to the weight ratio of 5.
The adding amount of the cellulase and the pectinase in the embodiment is 5 percent and 9 percent of the total amount of the mulberry residue respectively.
The temperature of the enzymolysis treatment in the embodiment is 32-35 ℃, and the enzymolysis time is 35-45min.
The preparation method of the chitosan solution in this example was:
adding chitosan into 2-3 times of sodium alginate solution, dispersing thoroughly, adding fructus Lycii polysaccharide extractant 1-5% of chitosan, and stirring thoroughly to obtain chitosan solution.
The mass fraction of the sodium alginate solution in the embodiment is 10-15%.
The preparation method of the lycium barbarum polysaccharide extracting agent comprises the following steps:
the lycium barbarum polysaccharide extracting agent is prepared by the steps of feeding the lycium barbarum into a pounding machine for pounding, adding 5 times of ethanol solvent for ultrasonic treatment, carrying out ultrasonic power of 150-210W, carrying out ultrasonic time of 20-30min, removing alcohol after finishing ultrasonic treatment, boiling and extracting in 4-9 times of water for 2-5h, finishing extraction, and filtering.
The preparation method of the flaxseed extract of the embodiment comprises the following steps: crushing flaxseed, freezing at-5 deg.C for 1-2 hr, thawing in deionized water, adding 10-15 times of ethanol organic solvent, extracting, and recovering ethanol solvent under reduced pressure to obtain flaxseed extractive solution.
In the dispersed thawing of the embodiment, the flaxseed is firstly thawed by using water with the temperature of 20 ℃, the flaxseed is completely thawed, and then the flaxseed is fully stirred and dispersed in the water.
The extraction treatment of this example employed a pressure of 10-20MPa, and the extraction temperature was 55-65 ℃.
Example 1.
The pig semen cultivation method for improving activity and fertility rate comprises the following steps:
the method comprises the following steps: mixing a mulberry modified fermentation material, lysine and a flaxseed extract according to a weight ratio of 1;
step two: then mixing the corn, the vitamins and the wheat bran according to a weight ratio of 4 to 1 to obtain a base material, and mixing the improved additive and the base material according to a weight ratio of 1 to 6 to prepare the special material for the pig;
step three: feeding pigs at 6 o' clock every day by adopting the special pig feed, feeding the pigs at 2.2Kg every day for 3 months, and collecting semen;
step four: and after semen is collected, heat preservation is carried out in a heat preservation box, and the heat preservation temperature is 20 ℃.
The preparation method of the mulberry modified fermentation material comprises the following steps:
s01: mashing mulberries into residues, adding cellulase and pectinase, and performing enzymolysis treatment to obtain an enzymolysis mulberry material after the enzymolysis is finished;
s02: carrying out enzymolysis on a mulberry material to inactivate enzyme, then placing the mulberry material in a fermentation tank for fermentation, adding lactobacillus accounting for 5% of the total amount of the enzymolysis material and glucose accounting for 5% of the total amount of the enzymolysis material, carrying out fermentation at 35 ℃ for 2d, and finishing the fermentation to obtain a mulberry fermentation material;
s03: and (3) preparing and blending the mulberry fermented material and the chitosan liquid according to the weight ratio of 5.
The adding amount of the cellulase and the pectinase in the embodiment is 5 percent and 9 percent of the total amount of the mulberry residue respectively.
The temperature of the enzymolysis treatment in this embodiment is 32 ℃, and the enzymolysis time is 35min.
The preparation method of the chitosan solution in this example was:
adding chitosan into 2 times of sodium alginate solution, dispersing thoroughly, adding fructus Lycii polysaccharide extractant 1% of chitosan, and stirring thoroughly to obtain chitosan solution.
The mass fraction of the sodium alginate solution in this example was 10%.
The preparation method of the lycium barbarum polysaccharide extracting agent comprises the following steps:
the preparation method comprises the steps of feeding medlar into a pounding machine for pounding, adding 5 times of ethanol solvent for ultrasonic treatment, wherein the ultrasonic power is 150W, the ultrasonic time is 20min, removing alcohol after ultrasonic treatment, boiling and extracting in 4 times of water for 2h, and filtering to obtain the medlar polysaccharide extracting agent after extraction.
The preparation method of the flaxseed extract of the present example comprises: crushing flaxseed, freezing at-5 deg.C for 1 hr, thawing in deionized water, adding 10 times of ethanol organic solvent, extracting, and recovering ethanol solvent under reduced pressure to obtain flaxseed extractive solution.
In the dispersed thawing of the embodiment, the flaxseed is firstly thawed by using water with the temperature of 20 ℃, the flaxseed is completely thawed, and then the flaxseed is fully stirred and dispersed in the water.
The extraction process of this example employed a pressure of 10MPa and an extraction temperature of 55 ℃.
Example 2.
The method for breeding the pig semen for improving the activity and the fertilization rate comprises the following steps:
the method comprises the following steps: mixing a mulberry modified fermentation material, lysine and a linseed extracting solution according to a weight ratio of 1;
step two: then mixing the corn, the vitamins and the wheat bran according to a weight ratio of 4 to 1 to obtain a base material, and mixing the improved additive and the base material according to a weight ratio of 1 to 6 to prepare the special material for the pig;
step three: feeding pigs at 6 o' clock every day by adopting the special pig feed, feeding the pigs at 2.2Kg every day for 3 months, and collecting semen;
step four: and after semen collection, carrying out heat preservation in a heat preservation box at the temperature of 25 ℃.
The preparation method of the mulberry modified fermentation material comprises the following steps:
s01: crushing mulberries into residues, adding cellulase and pectinase, and performing enzymolysis to obtain an enzymolysis mulberry material after the enzymolysis is finished;
s02: carrying out enzymolysis on a mulberry material, inactivating enzyme, then placing the mulberry material in a fermentation tank for fermentation, adding lactobacillus accounting for 10% of the total amount of the enzymolysis material and glucose accounting for 9% of the total amount of the enzymolysis material, carrying out fermentation at the temperature of 39 ℃ for 3d, and finishing the fermentation to obtain a mulberry fermentation material;
s03: and (3) preparing and blending the mulberry fermented material and the chitosan liquid according to the weight ratio of 5.
The adding amount of the cellulase and the pectinase in the embodiment is 5 percent and 9 percent of the total amount of the mulberry residues respectively.
The temperature of the enzymolysis treatment in this embodiment is 35 ℃, and the enzymolysis time is 45min.
The preparation method of the chitosan solution in this example was:
adding chitosan into 3 times of sodium alginate solution, dispersing thoroughly, adding Lycium barbarum polysaccharide extractant with 5% of total amount of chitosan, and stirring and mixing thoroughly to obtain chitosan solution.
The mass fraction of the sodium alginate solution in this example was 15%.
The preparation method of the lycium barbarum polysaccharide extracting agent comprises the following steps:
the preparation method comprises the steps of feeding medlar into a pounding machine for pounding, adding 5 times of ethanol solvent for ultrasonic treatment, wherein the ultrasonic power is 210W, the ultrasonic time is 30min, removing alcohol after ultrasonic treatment, boiling and extracting in 4-9 times of water for 5h, and filtering to obtain the medlar polysaccharide extracting agent after extraction.
The preparation method of the flaxseed extract of the present example comprises: crushing flaxseed, freezing at-5 deg.C for 2 hr, thawing in deionized water, adding 15 times of ethanol organic solvent, extracting, and recovering ethanol solvent under reduced pressure to obtain flaxseed extractive solution.
In the dispersed thawing of the embodiment, the flaxseed is firstly thawed by using water with the temperature of 20 ℃, the flaxseed is completely thawed, and then the flaxseed is fully stirred and dispersed in the water.
The extraction process of this example employed a pressure of 20MPa and an extraction temperature of 65 ℃.
Example 3.
The method for breeding the pig semen for improving the activity and the fertilization rate comprises the following steps:
the method comprises the following steps: mixing a mulberry modified fermentation material, lysine and a linseed extracting solution according to a weight ratio of 1;
step two: then mixing the corn, the vitamins and the wheat bran according to a weight ratio of 4 to 1 to obtain a base material, and mixing the improved additive and the base material according to a weight ratio of 1 to 6 to prepare the special material for the pig;
step three: feeding pigs at 6 o' clock every day by adopting the special pig feed, feeding the pigs at 2.2Kg every day for 3 months, and collecting semen;
step four: and after semen collection, carrying out heat preservation in a heat preservation box at the temperature of 22.5 ℃.
The preparation method of the mulberry modified fermentation material comprises the following steps:
s01: mashing mulberries into residues, adding cellulase and pectinase, and performing enzymolysis treatment to obtain an enzymolysis mulberry material after the enzymolysis is finished;
s02: carrying out enzymolysis on the mulberry material to remove enzyme, then placing the mulberry material in a fermentation tank for fermentation, adding lactobacillus accounting for 7.5% of the total amount of the enzymolysis material and glucose accounting for 7% of the total amount of the enzymolysis material, and finishing the fermentation at the fermentation temperature of 37 ℃ for 2.5 days to obtain a mulberry fermentation material;
s03: and (3) preparing and blending the mulberry fermented material and the chitosan liquid according to the weight ratio of 5.
The adding amount of the cellulase and the pectinase in the embodiment is 5 percent and 9 percent of the total amount of the mulberry residues respectively.
The temperature of the enzymolysis treatment in this example is 33.5 ℃, and the enzymolysis time is 40min.
The preparation method of the chitosan solution in this example was:
adding chitosan into 2.5 times of sodium alginate solution, dispersing thoroughly, adding Lycium barbarum polysaccharide extractant accounting for 3% of chitosan, and stirring and mixing thoroughly to obtain chitosan solution.
The mass fraction of the sodium alginate solution in this example was 12.5%.
The preparation method of the lycium barbarum polysaccharide extracting agent comprises the following steps:
the preparation method comprises the steps of feeding the medlar into a pounding machine for pounding, adding 5 times of ethanol solvent for ultrasonic treatment, wherein the ultrasonic power is 190W, the ultrasonic time is 25min, removing alcohol after ultrasonic treatment, boiling and extracting in 6 times of water for 3.5h, and filtering to obtain the medlar polysaccharide extracting agent.
The preparation method of the flaxseed extract of the embodiment comprises the following steps: crushing flaxseed, freezing at-5 deg.C for 1.5 hr, thawing in deionized water, adding 12.5 times of ethanol organic solvent, extracting, and recovering ethanol solvent under reduced pressure to obtain flaxseed extractive solution.
In the dispersed thawing of the embodiment, water with the temperature of 20 ℃ is adopted to thaw the flaxseeds firstly, the flaxseeds are completely thawed, and then the flaxseeds are stirred and dispersed in the water fully.
The extraction treatment of this example employed a pressure of 15MPa and an extraction temperature of 60 ℃.
Comparative example 1.
Different from the example 3, the mulberry modified fermentation material is not added.
Comparative example 2.
Different from the example 3, no chitosan solution was added to the mulberry modified fermentation material.
Comparative example 3.
The difference from the example 3 is that no lycium barbarum polysaccharide extractant is added in the preparation of the chitosan solution.
Comparative example 4.
The difference from the embodiment 3 is that the mulberry fermented material is prepared by replacing the raw material of liquorice with the fermented material of mulberry.
Comparative example 5.
Unlike example 3, no extract of flaxseed was added.
Comparative example 6.
The control is carried out without adopting special pig feed and is used as comparison before medication;
the products of examples 1-5 and comparative examples 1-3 were compared to comparative example 6; after/before the special pig feed is used, the sperm motility and the sperm aberration rate are tested in a contrast way;
the results of the performance measurements of examples 1 to 3 and comparative examples 1 to 5 are as follows
The materials in the embodiment 3 are treated, the sperm motility and the sperm aberration rate performance are excellent, no chitosan solution is added, no wolfberry polysaccharide extracting agent is added in the preparation of the chitosan solution, and the mulberry fermentation material adopts the liquorice raw material to replace the fermentation, so that the performance of the product is reduced, meanwhile, the linseed extracting solution is not added, the performance of the product is reduced, the linseed extracting solution and the mulberry modified fermentation material can play a role in coordination, the sperm motility is enhanced together, and the sperm aberration rate is reduced, so that the remarkable improvement effect is achieved;
the improved additive is prepared from the mulberry modified fermentation material, lysine and linseed extract in the process of breeding the pig semen, the mulberry modified fermentation material and the linseed extract are matched with a base material formed by corn, vitamins and wheat bran to be mixed to serve as a special nutrient material for breeding the pig semen, the activity and fertility rate of the pig semen can be enhanced by the aid of cooperation of the mulberry modified fermentation material and the linseed extract, the quality of the pig semen is improved, the mulberry fermentation material is matched with chitosan liquid in the process of preparing the mulberry modified fermentation material, the synergistic effect of the linseed extract can be further enhanced, and accordingly the activity and fertility rate of the extracted semen are stronger.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Furthermore, it should be understood that although the present specification describes embodiments, not every embodiment includes only a single embodiment, and such description is for clarity purposes only, and it is to be understood that all embodiments may be combined as appropriate by one of ordinary skill in the art to form other embodiments as will be apparent to those of skill in the art from the description herein.
Claims (10)
1. A pig semen cultivation method for improving activity and fertilization rate is characterized by comprising the following steps:
the method comprises the following steps: mixing a mulberry modified fermentation material, lysine and a linseed extracting solution according to a weight ratio of 1;
step two: then mixing the corn, the vitamins and the wheat bran according to a weight ratio of 4 to 1 to obtain a base material, and mixing the improved additive and the base material according to a weight ratio of 1 to 6 to prepare the special material for the pig;
step three: feeding pigs at 6 o' clock every day by adopting the special pig feed, feeding the pigs at 2.2Kg every day for 3 months, and collecting semen;
step four: and after semen is collected, heat preservation is carried out in a heat preservation box, and the heat preservation temperature is 20-25 ℃.
2. The pig semen cultivation method for improving activity and fertilization rate according to claim 1, wherein the preparation method of the mulberry modified fermentation material comprises the following steps:
s01: crushing mulberries into residues, adding cellulase and pectinase, and performing enzymolysis to obtain an enzymolysis mulberry material after the enzymolysis is finished;
s02: carrying out enzymolysis on a mulberry material, inactivating enzyme, then placing in a fermentation tank for fermentation, adding lactobacillus accounting for 5-10% of the total amount of the enzymolysis material and glucose accounting for 5-9% of the total amount of the enzymolysis material, fermenting at the temperature of 35-39 ℃ for 2-3d, and finishing the fermentation to obtain a mulberry fermentation material;
s03: and (3) preparing and blending the mulberry fermented material and the chitosan liquid according to the weight ratio of 5.
3. The method for cultivating boar semen with improved activity and fertility rate according to claim 2, wherein the adding amount of the cellulase and the pectinase is 5 percent and 9 percent of the total amount of the mulberry residues respectively.
4. The method for culturing porcine semen with improved activity and fertilization rate as claimed in claim 2, wherein the temperature of the enzymolysis is 32-35 ℃ and the enzymolysis time is 35-45min.
5. The method for culturing porcine semen with improved activity and fertilization rate as claimed in claim 2, wherein the chitosan solution is prepared by the following steps:
adding chitosan into 2-3 times of sodium alginate solution, dispersing thoroughly, adding fructus Lycii polysaccharide extractant 1-5% of chitosan, and stirring thoroughly to obtain chitosan solution.
6. The method for culturing pig semen with improved activity and fertilization rate as claimed in claim 5, wherein the sodium alginate solution is 10-15% by weight.
7. The method for culturing pig semen according to claim 5, wherein the fructus Lycii polysaccharide extract is prepared by the following steps:
the lycium barbarum polysaccharide extracting agent is prepared by the steps of feeding the lycium barbarum into a pounding machine for pounding, adding 5 times of ethanol solvent for ultrasonic treatment, carrying out ultrasonic power of 150-210W, carrying out ultrasonic time of 20-30min, removing alcohol after finishing ultrasonic treatment, boiling and extracting in 4-9 times of water for 2-5h, finishing extraction, and filtering.
8. The method for cultivating pig semen to increase activity and fertility rate according to claim 1, wherein the extract of flax seeds is prepared by: crushing flaxseed, freezing at-5 deg.C for 1-2 hr, thawing in deionized water, adding 10-15 times of ethanol organic solvent, extracting, and recovering ethanol solvent under reduced pressure to obtain flaxseed extractive solution.
9. The method as claimed in claim 8, wherein the dispersive thawing employs water of 20 ℃ to thaw the flaxseed first, and then the flaxseed is stirred in the water to disperse fully.
10. The method for culturing porcine semen with improved activity and fertilization rate as claimed in claim 8, wherein the extraction process is performed under a pressure of 10-20MPa and an extraction temperature of 55-65 ℃.
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