CN110583883A - Premix for improving boar sperm motility in summer and preparation method thereof - Google Patents
Premix for improving boar sperm motility in summer and preparation method thereof Download PDFInfo
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Abstract
The invention belongs to the technical field of pig feed, and particularly relates to a premix for improving sperm motility of boars in summer and a preparation method thereof. The feed comprises the following raw materials in parts by weight: 10-20 parts of astragalus membranaceus, 5-10 parts of epimedium, 3-5 parts of glossy privet fruit, 0.03-0.05 part of chromium picolinate, 0.02-0.05 part of zinc methionine, 0.03-0.05 part of lutein, 0.01-0.015 part of grape seed, 0.01-0.015 part of morinda officinalis, 0.008-0.01 part of schisandra chinensis, 0.002-0.004 part of 1,25 dihydroxyvitamin D, 0.003-0.005 part of trace element, 0.06-0.08 part of soybean lecithin and 0.08-0.1 part of chitosan nanoparticle suspension. The premix provided by the invention can improve the testosterone content in the boar body, improve the sperm motility and improve the immunity, and has good stability and membrane permeability, good absorption effect of the boar and quick response.
Description
Technical Field
The invention belongs to the technical field of pig feed, and particularly relates to a premix for improving sperm motility of boars in summer and a preparation method thereof.
Background
The boar is an important link in pig raising production, and the activity of the sperms is one of the marks which intensively embody the economic value of the boar and is also the most important mark. At present, the research on pig nutrition in the field of pig raising in China is more, but the research is mainly focused on growing pigs, fattening pigs and sows, and the research on boars is less. The quality of boar nutrition directly influences the semen quality of boars, and further influences the breeding of boars.
Summer heat stress is the biggest problem facing the production of boars at present. Due to the geographical position of China, most pig breeding areas in China are affected by high-temperature heat stress; particularly in southern areas, the boars are subjected to heat stress for a longer time and have higher stress, so that the breeding of the boars is seriously influenced, and huge economic loss is caused to farmers.
The high temperature of the boar is easy to deteriorate the sperm motility due to the physiological reasons. Boars with a humidity greater than 70% are heat stressed at temperatures above 26 c, and if the temperature is maintained for more than 10 weeks, boar semen quality will be greatly affected. The sperm motility of the boars is obviously reduced, and even part of the boars have dead sperm. Related researches find that the mechanism of the heat stress to cause significant influence on boar sperms is mainly as follows: after the boar is subjected to heat stress, a large amount of free radical ions are generated in vivo, high-concentration free radical ions cause hormone secretion disorder in the boar, and a large amount of oxygen radical ions enter testis through a blood testis barrier to cause damage to testis Sertoli cells and supporting cells, so that the generation of sperms is reduced, and the total number of sperms of the boar in summer is reduced. The oxygen free radical ion enters into epididymis after passing through a blood-testis barrier, thereby causing the mature disorder of boar sperms and further causing the low activity of the boar sperms. At present, a lot of researches for improving the heat stress capability of livestock are carried out.
For example, Chinese patent with application number CN201410338462.4 discloses a traditional Chinese medicine formula for resisting heat stress of breeding poultry in the breeding period, which is prepared from the following raw materials in percentage by weight: 15-20% of gypsum, 9-12% of rhizoma anemarrhenae, 8-12% of lophatherum gracile, 6-8% of artemisia anomala, 8-12% of astragalus membranaceus, 8-12% of bighead atractylodes rhizome, 8-12% of Chinese yam, 5-8% of hawthorn, 6-9% of herba epimedii, 5-8% of glossy privet fruit and the balance of liquorice to 100%. The invention can prevent and treat the influence of high temperature and high heat on the laying rate, the fertility rate and the hatching egg qualification rate in the breeding period of the breeding poultry in summer, and improve the breeding production and the economic benefit of the hatching eggs and the breeding poultry. The livestock feed additive adopts a pure traditional Chinese medicine composition with the functions of clearing away summer-heat, quenching thirst, promoting the production of body fluid, tonifying qi, activating blood circulation, invigorating spleen and strengthening kidney, and livestock can slowly absorb and convert the effective components of the traditional Chinese medicine, take effect for a long time and cannot relieve the heat stress of the livestock in a short time.
Also disclosed in chinese patent application No. CN201110042053.6 is an anti-heat stress composition for pigs, which comprises the following components in 1 kg: 15-60 g of L-ascorbic acid, 10-40 g of DL-alpha tocopheryl acetate, 10-40 g of yeast selenium, 0.05-0.2 g of chromium picolinate and 859.8-964.95 g of carrier. The composition can improve the heat stress resistance of pig from the direction of regulating normal secretion of cortical hormone and protecting cell membrane, and has poor effect of improving pig immune system.
Disclosure of Invention
In order to solve the technical problems in the prior art, the invention provides a premix for improving the sperm motility of boars in summer and a preparation method thereof, and the premix is realized by the following technical scheme:
a premixing agent for improving boar sperm motility in summer comprises the following raw materials in parts by weight: 10-20 parts of astragalus membranaceus, 5-10 parts of epimedium, 3-5 parts of glossy privet fruit, 0.03-0.05 part of chromium picolinate, 0.02-0.05 part of zinc methionine, 0.03-0.05 part of lutein, 0.01-0.015 part of grape seed, 0.01-0.015 part of morinda officinalis, 0.008-0.01 part of schisandra chinensis, 0.002-0.004 part of 1,25 dihydroxyvitamin D, 0.003-0.005 part of trace element, 0.06-0.08 part of soybean lecithin and 0.08-0.1 part of chitosan nanoparticle suspension.
Preferably, the trace elements are composed of calcium, iron, selenium and molybdenum according to a molar ratio of 2:1:1: 1.
Preferably, the preparation method of the chitosan nanoparticle comprises the following steps: dissolving water soluble chitosan in 0.5% citric acid solutionIn the solution, the pH value is adjusted to 6, and a chitosan mixed solution with the chitosan solution content of 2mg/ml is prepared; taking a proper amount of sodium tripolyphosphate to prepare 1mg/ml sodium tripolyphosphate aqueous solution; then at 750-－1Slowly dripping the sodium tripolyphosphate aqueous solution into the chitosan mixed solution at a rotating speed, carrying out ionic crosslinking and continuously stirring for 10-15min to prepare the chitosan nanoparticle suspension.
Preferably, the preparation method of the premix for improving the sperm motility of the boars in summer comprises the following steps:
(1) pulverizing radix astragali, herba Epimedii, and fructus Ligustri Lucidi, decocting in 8-10 times of water, and filtering to obtain decoction A and residue A;
(2) mixing grape seed, radix Morindae officinalis, and fructus Schisandrae, pulverizing, adding into the decoction A, stirring at 50-60 deg.C for 30-40min, and filtering to obtain Chinese medicinal extractive solution A and residue B; mixing residue A and residue B, adding complex microbial inoculum, fermenting at 25-30 deg.C for 20-24 hr, and sterilizing to obtain fermented product;
(3) adding trace elements into the Chinese medicinal extract A, adding 8-10 times of 70% methanol solution, mixing, reacting at 60-70 deg.C for 1-1.2 hr, and recovering methanol to obtain mixed extract A;
(4) adding 6-8 times of 80% ethanol into the fermented product, reflux extracting at 50-60 deg.C for 40-50min, filtering, recovering ethanol from the filtrate, and concentrating at 40-50 deg.C to obtain Chinese medicinal extract; mixing the traditional Chinese medicine extract and soybean lecithin, adding 1-2 times of anhydrous ethanol for compound reaction, and recovering ethanol to obtain a mixed extract B;
(5) mixing the mixed extract A and B, concentrating at 40-50 deg.C to obtain paste, spray drying to obtain powder, adding xanthophyll and chitosan nanoparticle suspension, and ultrasonic processing for 3-5min to obtain mixture; and adding chromium picolinate and zinc methionine, and mixing uniformly to obtain the premix.
Preferably, the composite microbial inoculum is a liquid microbial inoculum which contains lactobacillus acidophilus, bacillus subtilis and trichoderma; the viable count of Lactobacillus acidophilus is 6-8 × 108cfu/ml, viable count of bacillus subtilis is 4-6106cfu/ml, viable count of Trichoderma 6-8 × 104cfu/ml。
Preferably, in the step (2), the adding amount of the liquid microbial inoculum is 0.12-0.15% of the mixing mass of the filter residue A and the filter residue B.
Preferably, in the step (4), the conditions of the complex reaction are as follows: the reaction is carried out for 1.5 to 2 hours at the temperature of between 45 and 50 ℃ by magnetic stirring, and the magnetic stirring speed is 150-.
In the step (5), the ultrasonic treatment frequency is 25 to 30 kHz.
The invention has the beneficial effects that:
1. the combined use of the astragalus, the epimedium and the glossy privet fruit can improve the testosterone content in the boar body, improve the sperm motility, improve the immunocompetence and reduce the stress. The combined use of grape seeds, morinda officinalis and schisandra chinensis can reduce lipid peroxidation under high-temperature heat stress and improve the activity of superoxide dismutase in boars. Zinc methionine as the coenzyme of cAMP has important functions in sperm movement, formation and sperm maturation in epididymis, and can reduce the damage of boar sperm caused by heat stress to a certain extent. The lutein has the effects of promoting reproduction of germ cells and repairing cell membranes, can repair boar sperm plasma membrane damage caused by heat stress, and improve boar sperm activity. 1,25 dihydroxyvitamin D is used as a metabolite of the vitamin D to improve the utilization of calcium ions in boar sperms, increase the movement capacity of boar sperms and improve the sperm motility.
2. In the process of preparing the premix, the invention comprises the following steps: the decoction prepared from the astragalus, the epimedium and the glossy privet fruit is adopted to treat the rest traditional Chinese medicine components, which is beneficial to promoting the interaction of the effective components of the traditional Chinese medicines and improving the efficacy of the traditional Chinese medicines. The lactobacillus acidophilus, the bacillus subtilis and the trichoderma are adopted to treat the Chinese medicine residues, so that the dissolution rate of active ingredients in the Chinese medicine residues can be improved, and part of the active ingredients of the Chinese medicine can be converted into substances which can be easily absorbed by intestinal digestive systems of boars. The trace elements are adopted to react with the traditional Chinese medicine extracting solution A, and the active ingredients of the traditional Chinese medicine and the trace elements are combined to generate a complex, so that the biological activity of the active ingredients of the traditional Chinese medicine is enhanced; meanwhile, the microelements can also improve the immunity of the boar. The soybean lecithin is adopted to react with the traditional Chinese medicine extract B, and the traditional Chinese medicine active ingredients and the soybean lecithin generate a phospholipid compound, so that the stability and the membrane permeability of the traditional Chinese medicine active ingredients are improved, and the absorption of the traditional Chinese medicine active ingredients by boars is facilitated. The chitosan nanoparticle suspension is adopted to treat the mixed extract A, the mixed extract B and the lutein, so that the stability, the membrane permeability and the solubility of the effective ingredients of the traditional Chinese medicine and the lutein can be further improved, and the effective ingredients of the traditional Chinese medicine and the lutein of the boar can be further improved.
The premix for improving the sperm motility of the boars in summer provided by the invention can improve the testosterone content in the boars, improve the sperm motility and improve the immunocompetence, and has good stability and membrane permeability, good absorption effect of the boars and quick response.
Drawings
Fig. 1 is a graph showing the temperature change of the environment during the experiment period "1/9/2017-25/9/2017".
Detailed Description
The technical solution of the present invention is further defined below with reference to the specific embodiments, but the scope of the claims is not limited to the description.
Example 1
Premix for improving sperm motility of boars in summer
The components are as follows: 15kg of astragalus membranaceus, 8kg of epimedium herb, 4kg of glossy privet fruit, 0.035kg of chromium picolinate, 0.03kg of zinc methionine, 0.04g of lutein, 0.01kg of grape seeds, 0.01kg of morinda officinalis, 0.08kg of schisandra chinensis, 0.025kg of 1,25 dihydroxyvitamin D, 0.003kgg of trace elements, 0.06kg of soybean lecithin and 0.08kg of chitosan nanoparticle suspension.
The trace elements are composed of calcium, iron, selenium and molybdenum according to a molar ratio of 2:1:1: 1.
The preparation method of the chitosan nanoparticles comprises the following steps: dissolving water-soluble chitosan in 0.5% citric acid solution, adjusting pH to 6, and preparing into chitosan mixed solution with chitosan solution content of 2 mg/ml; taking a proper amount of sodium tripolyphosphate to prepare 1mg/ml sodium tripolyphosphate aqueous solution; then at 800 r.min－1Slowly dripping the sodium tripolyphosphate aqueous solution into the chitosan mixed solution at the rotating speed, carrying out ion crosslinking and continuously stirring for 10min to prepare the chitosan nanoparticle suspension.
The preparation method of the premix for improving the sperm motility of the boars in summer comprises the following steps:
(1) pulverizing radix astragali, herba Epimedii, and fructus Ligustri Lucidi, decocting in 8 times of water, and filtering to obtain decoction A and residue A;
(2) mixing grape seed, radix Morindae officinalis, and fructus Schisandrae, pulverizing, adding into the decoction A, stirring at 50-60 deg.C for 30min, and filtering to obtain Chinese medicinal extractive solution A and residue B; uniformly mixing the filter residue A and the filter residue B, adding a compound microbial inoculum with the mass of 0.12% of the filter residue A, fermenting at 25-30 ℃ for 20h, and sterilizing to obtain a fermentation product;
(3) adding trace elements into the Chinese medicinal extract A, adding 8-10 times of 70% methanol solution, mixing, reacting at 60-70 deg.C for 1 hr, and recovering methanol to obtain mixed extract A;
(4) adding 6 times of 80% ethanol into the fermented product, reflux extracting at 50-60 deg.C for 40min, filtering, recovering ethanol from the filtrate, and concentrating at 40-50 deg.C to obtain Chinese medicinal extract; mixing the Chinese medicinal extract and soybean phospholipid, adding 1 time of anhydrous ethanol, magnetically stirring at 45-50 deg.C for 1.5 hr, and recovering ethanol to obtain mixed extract B; wherein the magnetic stirring speed is 200 r/min;
(5) mixing the mixed extract A and B, concentrating at 40-50 deg.C to obtain paste, spray drying to obtain powder, adding xanthophyll and chitosan nanoparticle suspension, mixing, and treating with 25kHz ultrasonic wave for 5min to obtain mixture; and adding chromium picolinate and zinc methionine, and mixing uniformly to obtain the premix.
The composite microbial inoculum is a liquid microbial inoculum, which contains lactobacillus acidophilus, bacillus subtilis and trichoderma; the viable count of Lactobacillus acidophilus is 6-8 × 108cfu/ml, viable count of Bacillus subtilis is 4-6 × 106cfu/ml, viable count of Trichoderma 6-8 × 104cfu/ml。
Example 2
Premix for improving sperm motility of boars in summer
The components are as follows: 10kg of astragalus membranaceus, 9kg of epimedium herb, 3.5kg of glossy privet fruit, 0.03kgg kg of chromium picolinate, 0.025kg of zinc methionine, 0.05kg of lutein, 0.12kgg of grape seeds, 0.12kg of morinda officinalis, 0.09kg of schisandra chinensis, 0.02kgg of 1,25 dihydroxyvitamin D, 0.004kg of trace elements, 0.085kg of soybean lecithin and 0.09kg of chitosan nanoparticle suspension.
The trace elements are composed of calcium, iron, selenium and molybdenum according to a molar ratio of 2:1:1: 1.
The preparation method of the chitosan nanoparticles comprises the following steps: dissolving water-soluble chitosan in 0.5% citric acid solution, adjusting pH to 6, and preparing into chitosan mixed solution with chitosan solution content of 2 mg/ml; taking a proper amount of sodium tripolyphosphate to prepare 1mg/ml sodium tripolyphosphate aqueous solution; then at 750 r.min－1Slowly dripping the sodium tripolyphosphate aqueous solution into the chitosan mixed solution at the rotating speed, carrying out ion crosslinking and continuously stirring for 15min to prepare the chitosan nanoparticle suspension.
The preparation method of the premix for improving the sperm motility of the boars in summer comprises the following steps:
(1) pulverizing radix astragali, herba Epimedii, and fructus Ligustri Lucidi, decocting in 8-10 times of water, and filtering to obtain decoction A and residue A;
(2) mixing grape seed, radix Morindae officinalis, and fructus Schisandrae, pulverizing, adding into the decoction A, stirring at 50-60 deg.C for 30-40min, and filtering to obtain Chinese medicinal extractive solution A and residue B; mixing residue A and residue B, adding 0.12-0.15 wt% of compound microbial inoculum, fermenting at 25-30 deg.C for 20-24 hr, and sterilizing to obtain fermented product;
(3) adding trace elements into the Chinese medicinal extract A, adding 8-10 times of 70% methanol solution, mixing, reacting at 60-70 deg.C for 1-1.2 hr, and recovering methanol to obtain mixed extract A;
(4) adding 6-8 times of 80% ethanol into the fermented product, reflux extracting at 50-60 deg.C for 40-50min, filtering, recovering ethanol from the filtrate, and concentrating at 40-50 deg.C to obtain Chinese medicinal extract; mixing the Chinese medicinal extract and soybean phospholipid, adding 1-2 times of anhydrous ethanol, reacting at 45-50 deg.C under magnetic stirring for 1.5-2 hr, and recovering ethanol to obtain mixed extract B; wherein the magnetic stirring speed is 150-;
(5) mixing the mixed extract A and B, concentrating at 40-50 deg.C to obtain paste, spray drying to obtain powder, adding xanthophyll and chitosan nanoparticle suspension, mixing, and treating with ultrasonic at 25-30kHz for 3-5min to obtain mixture; and adding chromium picolinate and zinc methionine, and mixing uniformly to obtain the premix.
The composite microbial inoculum is a liquid microbial inoculum, which contains lactobacillus acidophilus, bacillus subtilis and trichoderma; the viable count of Lactobacillus acidophilus is 6-8 × 108cfu/ml, viable count of Bacillus subtilis is 4-6 × 106cfu/ml, viable count of Trichoderma 6-8 × 104cfu/ml。
Example 3
Premix for improving sperm motility of boars in summer
The components are as follows: 18kg of astragalus membranaceus, 7kgg of epimedium herb, 4.5kg of glossy privet fruit, 0.04kgg of chromium picolinate, 0.028kg of zinc methionine, 0.03kg of lutein, 0.15kg of grape seeds, 0.15kg of morinda officinalis, 0.10kg of schisandra chinensis, 0.03kg of 1,25 dihydroxyvitamin D, 0.005kg of trace elements, 0.08kg of soybean lecithin and 0.1kg of chitosan nanoparticle suspension.
The trace elements are composed of calcium, iron, selenium and molybdenum according to a molar ratio of 2:1:1: 1.
The preparation method of the chitosan nanoparticle suspension comprises the following steps: dissolving water-soluble chitosan in 0.5% citric acid solution, adjusting pH to 6, and preparing into chitosan mixed solution with chitosan solution content of 2 mg/ml; taking a proper amount of sodium tripolyphosphate to prepare 1mg/ml sodium tripolyphosphate aqueous solution; then at 750-－1Slowly dripping the sodium tripolyphosphate aqueous solution into the chitosan mixed solution at a rotating speed, carrying out ionic crosslinking and continuously stirring for 10-15min to prepare the chitosan nanoparticle suspension.
The preparation method of the premix for improving the sperm motility of the boars in summer comprises the following steps:
(1) pulverizing radix astragali, herba Epimedii, and fructus Ligustri Lucidi, decocting in 8-10 times of water, and filtering to obtain decoction A and residue A;
(2) mixing grape seed, radix Morindae officinalis, and fructus Schisandrae, pulverizing, adding into the decoction A, stirring at 50-60 deg.C for 30-40min, and filtering to obtain Chinese medicinal extractive solution A and residue B; mixing residue A and residue B, adding 0.12-0.15 wt% of compound microbial inoculum, fermenting at 25-30 deg.C for 20-24 hr, and sterilizing to obtain fermented product;
(3) adding trace elements into the Chinese medicinal extract A, adding 8-10 times of 70% methanol solution, mixing, reacting at 60-70 deg.C for 1-1.2 hr, and recovering methanol to obtain mixed extract A;
(4) adding 6-8 times of 80% ethanol into the fermented product, reflux extracting at 50-60 deg.C for 40-50min, filtering, recovering ethanol from the filtrate, and concentrating at 40-50 deg.C to obtain Chinese medicinal extract; mixing the Chinese medicinal extract and soybean phospholipid, adding 1-2 times of anhydrous ethanol, reacting at 45-50 deg.C under magnetic stirring for 1.5-2 hr, and recovering ethanol to obtain mixed extract B; wherein the magnetic stirring speed is 150-;
(5) mixing the mixed extract A and B, concentrating at 40-50 deg.C to obtain paste, spray drying to obtain powder, adding xanthophyll and chitosan nanoparticle suspension, mixing, and treating with ultrasonic at 25-30kHz for 3-5min to obtain mixture; and adding chromium picolinate and zinc methionine, and mixing uniformly to obtain the premix.
The composite microbial inoculum is a liquid microbial inoculum, which contains lactobacillus acidophilus, bacillus subtilis and trichoderma; the viable count of Lactobacillus acidophilus is 6-8 × 108cfu/ml, viable count of Bacillus subtilis is 4-6 × 106cfu/ml, viable count of Trichoderma 6-8 × 104cfu/ml。
Example 4
10kg of astragalus membranaceus, 5kg of epimedium herb, 5kg of glossy privet fruit, 0.03kg of chromium picolinate, 0.05kg of zinc methionine, 0.03kg of lutein, 0.01kg of grape seeds, 0.01kg of morinda officinalis, 0.01kg of schisandra chinensis, 0.002kg of 1,25 dihydroxyvitamin D, 0.003kg of trace elements, 0.06kg of soybean lecithin and 0.1kg of chitosan nanoparticle suspension.
The preparation method is the same as example 1.
Example 5
The components are as follows: 20kg of astragalus membranaceus, 10kg of epimedium herb, 3kg of glossy privet fruit, 0.05kg of chromium picolinate, 0.02-0.05 part of zinc methionine, 0.05kg of lutein, 0.015kg of grape seeds, 0.015kg of morinda officinalis, 0.008kg of schisandra chinensis, 0.004kg of 1,25 dihydroxyvitamin D, 0.005kg of trace elements, 0.08kg of soybean lecithin and 0.08kg of chitosan nanoparticle suspension.
The preparation method is the same as example 3.
Comparative example 1
The difference between the comparative example 1 and the example 1 is that no trace elements and soybean lecithin are added in the preparation process to react with the traditional Chinese medicine extracting solution A and the traditional Chinese medicine extract, but the traditional Chinese medicine extracting solution A and the traditional Chinese medicine extract are directly concentrated into paste, and then are spray-dried to prepare powder. The rest of the process is the same.
Comparative example 2
Comparative example 2 is different from example 1 in that the chitosan nanoparticle suspension is not added in step (5), and the rest of the process is the same.
Comparative example 3
The difference between the comparative example 3 and the example 1 is that the filter residue A and the filter residue B are directly mixed and extracted without adopting the complex microbial inoculum, and the rest processes are the same.
Experimental example 1
The research on the traditional Chinese medicine components in the premix comprises the following steps: the traditional Chinese medicine is prepared from Chinese herbal medicines with the functions of tonifying kidney and securing essence, such as astragalus, epimedium, glossy privet fruit, Chinese yam, eucommia bark, cistanche deserticola, Chinese dodder seed, rehmannia root, medlar, mulberry, Chinese yam, lotus seed, Chinese gall, malaytea scurfpea fruit and the like; mixing different Chinese medicinal formulas with the same amount of chromium picolinate, zinc methionine, lutein, grape seed, radix Morindae officinalis, fructus Schisandrae, 1,25 dihydroxyvitamin D, microelements, soybean lecithin and chitosan nanoparticle suspension, and grinding to obtain different premixes; then adding the premix into ration according to the weight ratio of 0.4% to feed the boars; feeding 2.5kg daily ration twice a day with normal drinking water. Experiment time: 1/9/2017-25/9/2017. And counting the sperm motility indexes of the boars after the experiment.
As a result, it was found that: by adopting the traditional Chinese medicine formula of astragalus, epimedium and glossy privet fruit, the average sperm motility of the boar can reach 82.15; is 0.05-18.32 percent higher than other traditional Chinese medicine compositions.
Experimental example 2
Experiment time: 1/9/2017-25/9/2017
Experimental materials: 180 duroc breeding boars were selected for the trial and divided into 11 groups of 20, of which 10 were experimental groups and the other group was control group. The experimental groups were fed with the diets of the experimental groups in table 1, wherein the premixes in the experimental groups 1-8 were the premixes prepared in examples 1-5 and comparative examples 1-3; the experimental groups 5-6 respectively feed Chinese patent with application number CN201410338462.4, which discloses a traditional Chinese medicine formula for resisting heat stress in breeding period of breeding poultry, and Chinese patent with application number CN201110042053.6, which discloses a heat stress resisting composition for pigs; the experimental groups were fed the diets of the control group in table 1. Feeding 2.5kg daily ration twice a day with normal drinking water. After statistical experiments, the energy and activity indexes of the boars in each group are detected, and the results are shown in table 2:
TABLE 1 Experimental daily formula
| Raw materials | Corn (corn) | Bean pulp | Wheat bran | Premix compound | Premix agent | Total up to |
| Control group | 66％ | 20％ | 4％ | 10％ | 0 | 100％ |
| Experimental group | 65.8％ | 19.8％ | 4％ | 10％ | 0.4％ | 100％ |
TABLE 2 average of post-test energy and vitality indices of boars
Note: weighing the amount of the semen by using an electronic balance; the sperm motility is measured by a sperm automatic analyzer; the sperm density is measured by a sperm automatic analyzer; the sperm motility rate is measured by a sperm automatic analyzer; effective sperm count is sperm amount x sperm density x sperm motility; total sperm count is the amount of sperm multiplied by sperm density.
As can be seen from the table, compared with the control group, the premixes used in the experimental groups 1 to 10 have the effect of the sperm motility and the survival rate of boars, wherein the effect of the experimental groups 1 to 5 is more significant, and the premixes are significantly different from the experimental groups 6 to 10.
It should be noted that the above examples and test examples are only for further illustration and understanding of the technical solutions of the present invention, and are not to be construed as further limitations of the technical solutions of the present invention, and the invention which does not highlight essential features and significant advances made by those skilled in the art still belongs to the protection scope of the present invention.
Claims (8)
1. The premix for improving the sperm motility of boars in summer is characterized by comprising the following raw materials in parts by weight: 10-20 parts of astragalus membranaceus, 5-10 parts of epimedium, 3-5 parts of glossy privet fruit, 0.03-0.05 part of chromium picolinate, 0.02-0.05 part of zinc methionine, 0.03-0.05 part of lutein, 0.01-0.015 part of grape seed, 0.01-0.015 part of morinda officinalis, 0.008-0.01 part of schisandra chinensis, 0.002-0.004 part of 1,25 dihydroxyvitamin D, 0.003-0.005 part of trace element, 0.06-0.08 part of soybean lecithin and 0.08-0.1 part of chitosan nanoparticle suspension.
2. The premix for improving boar summer sperm motility according to claim 1, wherein the trace elements are composed of calcium, iron, selenium and molybdenum according to a molar ratio of 2:1:1: 1.
3. The premix for improving boar summer sperm motility according to claim 1, wherein the preparation method of the chitosan nanoparticles comprises the following steps: dissolving water-soluble chitosan in 0.5% citric acid solution, adjusting pH to 6, and preparing into chitosan mixed solution with chitosan solution content of 2 mg/ml; taking a proper amount of sodium tripolyphosphate to prepare 1mg/ml sodium tripolyphosphate aqueous solution; then at 750-－1Slowly dripping the sodium tripolyphosphate aqueous solution into the chitosan mixed solution at a rotating speed, carrying out ionic crosslinking and continuously stirring for 10-15min to prepare the chitosan nanoparticle suspension.
4. The method for preparing the premix for improving the sperm motility of boars in summer according to claim 1, comprising the following steps:
(1) pulverizing radix astragali, herba Epimedii, and fructus Ligustri Lucidi, decocting in 8-10 times of water, and filtering to obtain filtrate A and residue A;
(2) mixing grape seed, radix Morindae officinalis, and fructus Schisandrae, pulverizing, adding into filtrate A, stirring at 50-60 deg.C for 30-40min, and filtering to obtain Chinese medicinal extractive solution A and residue B; mixing residue A and residue B, adding complex microbial inoculum, fermenting at 25-30 deg.C for 20-24 hr, and sterilizing to obtain fermented product;
(3) adding trace elements into the Chinese medicinal extract A, adding 8-10 times of 70% methanol solution, mixing, reacting at 60-70 deg.C for 1-1.2 hr, and recovering methanol to obtain mixed extract A;
(4) adding 6-8 times of 80% ethanol into the fermented product, reflux extracting at 50-60 deg.C for 40-50min, filtering, recovering ethanol from the filtrate, and concentrating at 40-50 deg.C to obtain Chinese medicinal extract; mixing the traditional Chinese medicine extract and soybean lecithin, adding 1-2 times of anhydrous ethanol for compound reaction, and recovering ethanol to obtain a mixed extract B;
(5) mixing the mixed extract A and B, concentrating at 40-50 deg.C to obtain paste, spray drying to obtain powder, adding xanthophyll and chitosan nanoparticle suspension, and ultrasonic processing for 3-5min to obtain mixture; and adding chromium picolinate and zinc methionine, and mixing uniformly to obtain the premix.
5. The method for preparing the premix for improving the sperm motility of boars in summer according to claim 4, wherein in the step (4), the condition of the complex reaction is as follows: the reaction is carried out for 1.5 to 2 hours at the temperature of between 45 and 50 ℃ by magnetic stirring, and the magnetic stirring speed is 150-.
6. The method for preparing the premix for improving boar summer sperm motility according to claim 4, wherein the compound microbial inoculum is a liquid microbial inoculum which contains lactobacillus acidophilus, bacillus subtilis and trichoderma.
7. The method of claim 6, wherein the complex microbial inoculum comprises viable Lactobacillus acidophilusThe number of the particles is 6-8 multiplied by 108cfu/ml, viable count of Bacillus subtilis is 4-6 × 106cfu/ml, viable count of Trichoderma 6-8 × 104cfu/ml。
8. The method for preparing the premix for improving the sperm motility of boars in summer according to claim 4, wherein in the step (2), the addition amount of the liquid microbial inoculum is 0.12-0.15% of the mixing mass of the filter residue A and the filter residue B.
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