CN102630824B - Plant extract compound feed additive - Google Patents
Plant extract compound feed additive Download PDFInfo
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- CN102630824B CN102630824B CN 201210142994 CN201210142994A CN102630824B CN 102630824 B CN102630824 B CN 102630824B CN 201210142994 CN201210142994 CN 201210142994 CN 201210142994 A CN201210142994 A CN 201210142994A CN 102630824 B CN102630824 B CN 102630824B
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Abstract
The invention discloses a plant extract compound feed additive, which belongs to the field of feeds, and particularly relates to plant extract compound feed additives. The plant extract compound feed additive comprises the following components by mass: 20 to 40 percent of Achyranthes bidentata polysaccharides, 20 to 30 percent of Perilla extract, 15 to 25 percent of yeast cell wall extract, 20 to30 percent of Bacillus subtilis freeze-dried powder, and 1 to 5 percent of fructo-oligosaccharide, wherein Bacillus subtilis adopts CGMCC No 6012. The plant extract compound feed additive can effectively reduce the usage amount of antibiotic medicines in domesticated animals, improve the immunity of the domesticated animals, improve the safety of animal meat products, improve the feed utilization efficiency of animals, reduce the usage amount of antibiotics and medicines, enhance the resistance of animals, and improve the feeding benefits.
Description
Technical field:
The invention belongs to field of fodder, particularly the plant extracts composite feed additive.
Background technology:
In modern livestock and poultry cultivation process, in order to prevent and treat Animal diseases, often guarantee animal health with excessive antibiotic product, but excessive antibiotic use often causes the quality of animal meat product and product thereof to descend and the enhancing of bacteria antibiotic patience, and the transmission by food chain causes human health also to be subject to having a strong impact on of antibiotic problem.
Green feed additive take Inokopolyose etc. as representative becomes the developing direction of feed addictive from now on; Inokopolyose is a kind of water-soluble polysaccharide that extracts in the root of the amaranthaceous plant root of bidentate achyranthes, and good water solubility is easily absorbed by body, and has immunological regulation, the biological function widely such as anti-oxidant, is regarded as the natural green additive of substitute antibiotics.
The feed addictive of the present invention exploitation is take Inokopolyose etc. as primary raw material, and composite forming is a kind of green feed additive product of substitute antibiotics series products.
Summary of the invention:
The invention provides a Plant Extracts composite feed additive;
Described plant extracts composite feed additive consists of:
Inokopolyose 20-40%, extractive of perilla 20-30%, yeast cell wall extract 15-25%, bacillus subtilis freeze-dried vaccine powder 20-30%, FOS 1-5%; Bacillus subtilis (Bacillus subtilis) is CGMCC6012, and aforementioned proportion is mass percent.
Described extractive of perilla preparation method is as follows: perilla seed added the extraction of 7-10 times of weight absolute ethyl alcohol dipping of perilla seed after pulverizing the 40-60 mesh sieve, control 30-45 ℃ of temperature, adjust temperature after 1-2 hour and be 55-60 ℃ 2-3 hour, extract is concentrated, drying obtains ethanol extract; Add 75～85 ℃ of hot water in perilla seed residue after alcohol extract, the hot water addition is 3-6 times of perilla seed residue weight, processing time 30-50 minute, extract 2-3 time continuously, and with spray-drying after the extract Vacuum Concentration, obtain the hot water extract; Above-mentioned ethanol extract and hot water extract are merged pulverizing, cross 60 mesh sieves, namely get extractive of perilla.
Described Inokopolyose preparation method is as follows:
Cross the 30-60 mesh sieve after radix cyathulae is pulverized, interpolation radix cyathulae weight 3-6 doubly measures 70-85 ℃ of hot water and extracts 2-3 time continuously, each 30-50 minute; Collect extract, add ethanol in the extract and make that in extract, concentration of alcohol reaches 70-85%, kept 2-3 hour, collecting precipitation 60-75 ℃ crushed after being dried, mistake 40 mesh sieves namely get Inokopolyose.
Described bacillus subtilis is bacillus subtilis CGMCC6012, and in the freeze-dried vaccine powder, number of viable is 2-8*10
8Individual/g.
Described plant extracts composite feed additive is made by following methods:
Yeast cell wall extract and FOS were pulverized the 50-60 mesh sieve, and above-mentioned crushed material and Inokopolyose and extractive of perilla and bacillus subtilis freeze-dried vaccine powder proportionally are mixed to get the plant extracts composite feed additive.
The invention provides a kind of Feed Manufacturing bacillus subtilis (Bacillus subtilis) bacterial classification, this bacterial strain has been preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on April 16th, 2012, the preservation address is positioned at the Yard 1, BeiChen xi Road, Chaoyang District, Beijing City No. 3, (postcode 100101), deposit number are CGMCC No6012.
Beneficial effect:
FOS refers to that 2～5 fructosyls are chain link, and the end group take a glucosyl group as chain links the carbohydrate that forms take fructosyl → fructose connecting key as main body framework.Refer to namely that 1～4 fructosyl is connected to β-2,1 key on the D-Fructose base of sucrose and the ketose (GF2) that forms, Nystose (GF3), GF4 (GF4) and the sugarcane mixture of six sugar (GF5) really.FOS is a kind of good water-soluble dietary fiber, can also impel Bifidobacterium to breed rapidly, thereby suppresses the growth of harmful bacteria, safeguards the gut flora balance; Can natural synthetic vitamin B1 in enteron aisle, B2, B6, B12, nicotinic acid and folic acid, thereby improve the function of enteron aisle, improve immunity and premunition.
The medicinal plant purple perilla is the band leaf spray of labiate purple perilla [Perilla frutescens(L.) Brltt.var.arguta Benth.Hand.-Mazz.].Be used as medicine so that stem, leaf and son are real.Purple perilla belongs to the comprehensive crossover commodity, and is both pharmaceutically acceptable, is clinical common medicine, can eat again.The form of being used as medicine claims lac encrusted twig with stem, and leaf claims again Su Ye, induces sweat, and son claims again perillaseed, FRUCTUS PERILLAE, red perillaseed, is the important component of Suzi Jiangqi Tang.Expelling cold and relieving exterior syndrome, regulating the flow of QI to ease the stomach.Cure mainly cold, fever, be afraid of cold, lossless, uncomfortable in chest, the poisoning stomachache that causes of crab is separated in cough, diarrhoea, the diseases such as vomiting.
In yeast cell wall extract, the functional form carbohydrate of yeast cells outer wall can in conjunction with multiple mycotoxin, improve animal health by absorbing poisonous substance and pathogen to cell membrane.
Yeast cell wall extract is take saccharomyces cerevisiae as raw material, and through refining class fungal extracts that forms of technique such as breaking-wall cell, enzymolysis, separating-purifying and dryings, finished product is generally light grey powder to Dark grey.Studies confirm that, yeast cell wall is divided into 3 layers: outer is manna oligosacchride and protein conjugate, and the intermediate layer is β-(1,3), β-(1,6) glucan, and internal layer is chitin.Its special configuration can form special complementary structure with multiple mycotoxin, thereby is combined firmly with multiple mycotoxin, and discharges outside animal body by enteron aisle.
Bacillus subtilis: have following function: 1. oxygen consumption produces acid, and the regulating intestinal canal microecological balance reaches the diseases such as prevention diarrhoea, diarrhea; 2. secrete multiple enzyme, improve the feed digestive utilization ratio; 3. generation various nutrients participates in body metabolism; 4. raising animal immunizing power.
Perilla seed of the present invention carries out pulverize and break cellular wall to be processed, make in perilla seed function factor realize full price stripping and high efficiency extraction utilization, adopt respectively organic solvent and hot water to realize wherein effective extraction of different efficacies composition, particularly control the temperature section extraction and effectively improved especially extract yield, improved extraction efficiency and the quality of the extract of perilla seed.
The present invention is composite by science, bacillus subtilis, purple perilla, FOS, Inokopolyose have been realized organic assembling, product of the present invention can effectively reduce the usage quantity of antibiotics in letting animals feed, improve the immunity of letting animals feed, improve the security of animal meat product, improve the food utilization efficiency of animal, the use of antibiotic and medicine in the conventional raising of minimizing, strengthen the resistivity of animal, improve raise benefit.
Specific implementation method:
The following examples can make the present invention of those skilled in the art comprehend, but do not limit the present invention in any way.Bacterial screening method:
Induction mutation of bacterium
Take the ultraviolet mutagenesis method: take CICC10023 as starting strain, make bacteria suspension, coat on the culture medium plate, cultivate in 37 ℃ of incubators after ultraviolet irradiation 90s.
Bacterial screening
Choose 30 single bacterium colonies of looking strong from the 90s plate, be inoculated on the inclined-plane, in 37 ℃ of incubators, cultivated 24 hours, 0--4 ℃ stored refrigerated; With original strain in contrast, adopt conventional herd breeding condition CMC as carbon source and proteolysis circle method screening cellulase-producing and the strong bacterial strain of protease, screening obtains cellulase-producing and strong bacterial strain bacillus subtilis (Bacillus subtilis) L1 of protease, this bacterial strain cellulase-producing can improve 25% by the force rate original strain, produces protease and can improve 52% by the force rate original strain.In the center preservation of Chinese common micro-organisms culture presevation administration committee's common micro-organisms, deposit number is CGMCC No 6012 with this bacterial classification.
Culture medium forms: peptone 5 grams; Beef extract 3 grams; Sodium chloride 5 grams; Agar 20 grams; 1000 milliliters of distilled water; PH7.0.In the present invention, " yeast cell wall extract " is available from Hai Site group company or other companies.
Embodiment 1:
10 kilograms of plant extracts composite feed additives consist of:
Inokopolyose 30%, extractive of perilla 25%, yeast cell wall extract 20%, bacillus subtilis freeze-dried vaccine powder 23%, FOS 2%; Bacillus subtilis is CGMCC6012, and aforementioned proportion is mass percent.
Described extractive of perilla preparation method is as follows: perilla seed is pulverized and is added 8 times of weight absolute ethyl alcohols dippings of perilla seed after 50 mesh sieves and extract, 40 ℃ of temperature 2 hours, and rear adjustment temperature is 58 ℃ of time 2 hs, extract Vacuum Concentration, drying obtain ethanol extract; Add 80 ℃ of hot water in perilla seed residue after alcohol extract, the hot water addition is 5 times of perilla seed residue weight, 40 minutes processing times, extract continuously 2 times, and with spray-drying after the extract Vacuum Concentration, obtain the hot water extract; Above-mentioned ethanol extract and hot water extract are merged pulverizing, cross 60 mesh sieves, namely get extractive of perilla;
Described Inokopolyose preparation method is as follows:
Cross 40 mesh sieves after radix cyathulae is pulverized, add 80 ℃ of hot water of 4 times of amounts of radix cyathulae weight and extract 2 times continuously, each 40 minutes; Collect extract, add ethanol in the extract and make that in extract, concentration of alcohol reaches 75%, control temperature and kept 2 hours at 25 ℃, 75 ℃ of crushed after being dried of collecting precipitation are crossed 40 mesh sieves, namely get Inokopolyose;
Described bacillus subtilis is that bacillus subtilis is CGMCC6012 freeze-dried vaccine powder, and number of viable is 5*10
8Individual/g;
Described plant extracts composite feed additive is made by following methods:
Yeast cell wall extract and FOS were pulverized 60 mesh sieves, above-mentioned crushed material and Inokopolyose and extractive of perilla and bacillus subtilis freeze-dried vaccine powder were proportionally mixed being mixed to get the plant extracts composite feed additive.
This product bacillus subtilis used (Bacillus subtilis) bacterial classification, this bacterial strain has been preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on April 16th, 2012, the preservation address is positioned at the Yard 1, BeiChen xi Road, Chaoyang District, Beijing City No. 3, (postcode 100101), deposit number are CGMCC No6012.
The preparation of bacillus subtilis microbial agent:
1. the acquisition of zymotic fluid: adopt slant strains to spread cultivation step by step and obtain fermentation of bacillus subtilis liquid;
(1) first order seed is cultivated: in bacillus subtilis CGMCC6012 slant strains access 500 ml shake flasks, and 100 milliliters of culture medium loading amounts, 180 rev/mins of rotary shaking tables, 30 ℃ of cultivation temperature, incubation time 24 hours;
(2) secondary seed is cultivated: according in 500 milliliters of secondary seed shaking flasks of inoculum concentration access of 10%, condition of culture is identical with first order seed with first order seed;
(3) three grades of seed culture: secondary seed is accessed in 5000 milliliters of three grades of seed shaking flasks 1000 milliliters of culture medium loading amounts, 100 rev/mins of rotary shaking tables, 30 ℃ of cultivation temperature, incubation time 24 hours with 10% inoculum concentration;
(4) first class seed pot is cultivated: with the first class seed pot of three grades of seeds take 10% inoculum concentration access total measurement (volume) as 150L, fermentation medium loading amount 100L, 28 ℃ of cultivation temperature, 100 rev/mins of mixing speeds, ventilation (V/V) 1:0.5, tank pressure 0.05Mpa, incubation time 24 hours;
(5) fermented and cultured: the first class seed pot bacterial classification is accessed total measurement (volume) as 1.5 tons of secondary seed tanks take 10% inoculum concentration, 1 ton of fermentation medium loading amount, 28 ℃ of condition of culture cultivation temperature, 100 rev/mins of mixing speeds, ventilation (V/V) 1:0.5, tank pressure 0.05Mpa, incubation time 24 hours is cultivated and is finished bacteria concentration 11 * 10
8Individual/ml.
(6) centrifugation: adopt centrifuge to separate and obtain thalline.
(7) vacuum freeze drying: adopt drying process with atomizing to process the acquisition dry bacteria after adding protective agent; Protective agent consists of defatted milk 10%, lactose 5%, glycerine 5%.Vacuum freeze-drying technique is write the culture presevation handbook referring to Institute of Microorganism, Academia Sinica, publishes in 1980. 610-653.
Culture medium forms: peptone 5 grams; Beef extract 3 grams; Sodium chloride 5 grams; Agar 20 grams; 1000 milliliters of distilled water; PH7.0.
The product result of use
The inventive example 1 feed addictive result of use test
Test method:
Select the Dongguan City twins subordinate of feed corporation,Ltd pig farm, select each 50 of age in days, body weight close healthy sow, piglet and boars, all be divided at random 2 groups (test group and control groups), test group is used product of the present invention, and control group does not add product of the present invention; The addition of product of the present invention is the 1-2% of feed consumption, feeds continuously 60 days.Compare with control group, use
The invention feed addictive can obtain following effect:
(1) number born of sow on average increases by 0.7;
(2) the boar sperm count increases by 20%～25%, improves conception rate;
(3) before and after the childbirth, the prevention of sow constipation number of times reduces more than 35%;
(4) the weanling pig weight average increases to 6.3kg from 5.4kg, increases by 10% than control group weight average;
(5) the antibiotic use amount has reduced 80-95% the piglet phase;
The above results shows that product of the present invention can improve sow, piglet and boar body immunity, reduces antibiotic dosage, increases the cultivation quality and benefits.
Embodiment 2: implementation method is substantially with example 1;
10 kilograms of plant extracts composite feed additives consist of:
Inokopolyose 20%, extractive of perilla 30%, yeast cell wall extract 15%, bacillus subtilis freeze-dried vaccine powder 30%, FOS 5%; Bacillus subtilis is CGMCC6012, and aforementioned proportion is mass percent.
Embodiment 3: implementation method is substantially with example 1;
10 kilograms of plant extracts composite feed additives consist of:
Inokopolyose 40%, extractive of perilla 20%, yeast cell wall extract 16%, bacillus subtilis freeze-dried vaccine powder 23%, FOS 1%; Bacillus subtilis is CGMCC6012, and aforementioned proportion is mass percent.
Embodiment 4: implementation method is substantially with example 1;
10 kilograms of plant extracts composite feed additives consist of:
Inokopolyose 28%, extractive of perilla 22%, yeast cell wall extract 16%, bacillus subtilis freeze-dried vaccine powder 30%, FOS 4%; Bacillus subtilis is CGMCC6012, and aforementioned proportion is mass percent.
Claims (6)
1. a Plant Extracts composite feed additive, it is characterized in that described plant extracts composite feed additive consists of: Inokopolyose 20-40%, extractive of perilla 20-30%, yeast cell wall extract 15-25%, bacillus subtilis (Bacillus subtilis) freeze-dried vaccine powder 20-30%, FOS 1-5%; Bacillus subtilis (Bacillus subtilis) is CGMCC6012, aforementioned proportion is mass percent, described extractive of perilla preparation method is as follows: perilla seed added the extraction of 7-10 times of weight absolute ethyl alcohol dipping of perilla seed after pulverizing the 40-60 mesh sieve, temperature 30-45 ℃ adjust after 1-2 hour temperature be 55-60 ℃ 2-3 hour, extract is concentrated, drying obtains ethanol extract; Add 75～85 ℃ of hot water in perilla seed residue after alcohol extract, the hot water addition is 3-6 times of perilla seed residue weight, processing time 30-50 minute, extract 2-3 time continuously, and with spray-drying after the extract Vacuum Concentration, obtain the hot water extract; Above-mentioned ethanol extract and hot water extract are merged pulverizing, cross 60 mesh sieves, namely get extractive of perilla; Described Inokopolyose preparation method crosses the 30-60 mesh sieve after radix cyathulae is pulverized, and interpolation radix cyathulae weight 3-6 doubly measures 70-85 ℃ of hot water and extracts 2-3 time continuously, each 30-50 minute; Collect extract, add ethanol in the extract and make that in extract, concentration of alcohol reaches 70-85%, kept 2-3 hour, collecting precipitation 60-75 ℃ crushed after being dried, mistake 40 mesh sieves namely get Inokopolyose.
2. a Plant Extracts composite feed additive according to claim 1; It is characterized in that described bacillus subtilis CGMCC6012 freeze-dried vaccine powder number of viable is 2-8*10
8Individual/g.
3. a Plant Extracts composite feed additive according to claim 2; It is characterized in that described bacillus subtilis CGMCC6012 freeze-dried vaccine powder number of viable is 5*10
8Individual/g.
4. a Plant Extracts composite feed additive according to claim 1; It is characterized in that described plant extracts composite feed additive consists of: Inokopolyose 40%, extractive of perilla 20%, yeast cell wall extract 16%, bacillus subtilis freeze-dried vaccine powder 23%, FOS 1%; Bacillus subtilis is CGMCC6012.
5. a Plant Extracts composite feed additive according to claim 1; It is characterized in that described plant extracts composite feed additive consists of: Inokopolyose 28%, extractive of perilla 22%, yeast cell wall extract 16%, bacillus subtilis freeze-dried vaccine powder 30%, FOS 4%, bacillus subtilis is CGMCC6012.
6. a Plant Extracts composite feed additive according to claim 1, it is characterized in that described plant extracts composite feed additive consists of: Inokopolyose 20%, extractive of perilla 30%, yeast cell wall extract 15%, bacillus subtilis freeze-dried vaccine powder 30%, FOS 5%, bacillus subtilis are CGMCC6012, and aforementioned proportion is mass percent.
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103392946B (en) * | 2013-08-22 | 2014-09-03 | 广南(湛江)家丰饲料有限公司 | Pig feed additive capable of effectively improving immunity and intestinal health |
| CN103478414B (en) * | 2013-09-11 | 2015-01-21 | 湖南大北农农业科技有限公司 | Feed rich in conjugated linoleic acid and preparation method of feed |
| CN103609858A (en) * | 2013-11-20 | 2014-03-05 | 宁夏天地经纬电力设备工程有限公司 | Feed additive and preparation method thereof |
| CN104719632A (en) * | 2013-12-19 | 2015-06-24 | 天津工业大学 | Feed additive and preparation method thereof |
| CN103749977A (en) * | 2013-12-28 | 2014-04-30 | 邵素英 | Efficient feed additive |
| CN104366020A (en) * | 2014-09-28 | 2015-02-25 | 天津天绿健科技有限公司 | Feed additive product |
| CN109287872A (en) * | 2018-11-29 | 2019-02-01 | 佛山市南海东方澳龙制药有限公司 | For improving the additive and its preparation method and application of poultry immunity of organisms |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1096952A (en) * | 1993-07-02 | 1995-01-04 | 梁述尧 | The prescription of curing fowl diseases and manufacture method |
| CN1903058A (en) * | 2005-07-27 | 2007-01-31 | 湖南中业科技发展有限公司 | Ternary active microbiological prepns. for animals and birds |
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2012
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1096952A (en) * | 1993-07-02 | 1995-01-04 | 梁述尧 | The prescription of curing fowl diseases and manufacture method |
| CN1903058A (en) * | 2005-07-27 | 2007-01-31 | 湖南中业科技发展有限公司 | Ternary active microbiological prepns. for animals and birds |
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