CN114053204A - Anti-aging cream containing Changbai mountain wild ginseng cell water extract - Google Patents
Anti-aging cream containing Changbai mountain wild ginseng cell water extract Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/04—Plant cells or tissues
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
Abstract
The invention discloses an anti-aging cream containing a Changbai mountain wild ginseng cell water extract and a preparation method thereof, belonging to the technical field of cosmetics, and the anti-aging cream mainly comprises the following components: acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer, butanediol, glycerol, xanthan gum, EDTA-2Na, allantoin, dipotassium glycyrrhizinate, betaine, trehalose, hydrogenated lecithin, PEG-2 methyl glucose sesquistearate, caprylic/capric triglyceride, polydimethylsiloxane, tocopheryl acetate, propyl hydroxybenzoate, squalane, cetyl stearate alcohol, jojoba seed oil, cyclopenta dimethyl siloxane, tetrahydroxypropyl ethylenediamine, a mixed solution of phenoxyethanol and ethylhexyl glycerol, hydroxyethyl piperazine ethanesulfonic acid, nicotinamide, panthenol, essence, and mountain ginseng Changbai mountain wild herb cell water extract and the like.
Description
Technical Field
The invention belongs to the technical field of skin care products, and particularly relates to an anti-aging cream containing a Changbai mountain wild ginseng cell water extract.
Background
Skin aging is mainly divided into two forms of natural aging and photoaging, and natural aging is mainly caused by genetic factors and is obviously characterized by the appearance of wrinkles and the laxity of skin. Photoaging refers to the accumulation of ultraviolet damage during skin aging, and is the result of the combined action of natural aging and ultraviolet radiation. Skin aging is mainly characterized by the deterioration of the protective ability and the regulating ability of skin to the body, appearance changes such as skin color, form, color and luster and the like. With aging, the exposed part of skin is coarse and elastic, wrinkles are deepened and thickened, irregular pigmentation, vasodilation, epidermal keratosis, dermal elasticity fibrosis, degradation product accumulation and the like.
With the improvement of living standard of people, anti-aging becomes a persistent theme, and the market of anti-aging skin care products is rapidly developed. Although various anti-aging skin care products which are full in the existing market still cannot meet the requirements of different social people, the research of an anti-aging product has great significance to the market.
The Changbai mountain ginseng is one of natural treasures bred naturally, grows in an original forest with the altitude of one thousand hundred meters to two thousand meters, has the effects of tonifying five internal organs, calming the mind, calming the soul, stopping fright, removing pathogenic qi, improving eyesight, benefiting heart, benefiting intelligence, prolonging life after long-term use, has a history of thousands of years in application in China, is more and less at present, has higher requirements on the growth environment and very slow growth in a deep mountain, and has the root weight of 60-100 years which is dozens of grams. Wild ginseng is difficult to harvest, and the longer the growth period is, the better the drug effect is. The tonic effect is remarkable due to the rare yield, and the tonic tea has high reputation in the world, and the use of the tonic tea is limited due to the rare quantity, particularly the wild ginseng in the Changbai mountain for hundreds of years. Based on that each undifferentiated cell potentially contains all components of the ginseng, the research on obtaining the anti-aging cream containing the water extract of the ginseng cells has not been reported yet by carrying out amplification culture on the ginseng cells through a plant cell culture technology.
Disclosure of Invention
In view of the above, the invention aims to provide an anti-aging cream containing a water extract of Changbai mountain wild ginseng cells and a preparation method thereof.
The purpose of the invention is realized by the following modes:
the invention provides anti-aging cream containing a Changbai mountain wild ginseng cell water extract, which comprises the following components in parts by weight:
50-75 parts of pure water, 10-25 parts of humectant, 0.2-0.8 part of thickener, 0.05-0.4 part of chelating agent, 8-20 parts of emollient, 3-6 parts of emulsifier, 1-10 parts of skin conditioner, 0.1-10 parts of Changbai mountain wild ginseng cell water extract, 0.02-0.4 part of essence and 0.1-0.8 part of preservative.
Preferably, the humectant is one or a combination of more than two of glycerin, propylene glycol, butanediol, 1, 2-hexanediol, polyglutamic acid, sodium hyaluronate, trehalose and betaine.
Preferably, the thickening agent is one or a composition of more than two of xanthan gum, acrylic acid/C10-30 alkanol acrylate cross-linked polymer, acryloyl dimethyl ammonium taurate/VP copolymer and cetearyl alcohol.
Preferably, the chelating agent is one or a combination of more than two of EDTA-2Na and tetrahydroxypropyl ethylenediamine.
Preferably, the emollient is one or more of squalane, jojoba oil, caprylic/capric triglyceride, polydimethylsiloxane, tocopheryl acetate, and cyclopentadimethylsiloxane.
Preferably, the emulsifier is one or more of hydrogenated lecithin, potassium cetyl phosphate, PEG-20 methyl glucose sesquistearate, polysorbate-20, and polyglycerol-3 methyl glucose distearate.
Preferably, the skin conditioning agent is one or a combination of more than two of panthenol, nicotinamide, dipotassium glycyrrhizinate, allantoin and hydroxyethyl piperazine ethane sulfonic acid.
Preferably, the essence is selected from one or a composition of more than two of flower fragrance, fruit fragrance, green fragrance and costus root.
Preferably, the preservative is one or a combination of more than two of methyl paraben, propyl paraben, phenoxyethanol and ethylhexyl glycerin.
Preferably, the anti-aging cream comprises the following components in percentage by weight: 0.05-0.3% of acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer, 2-6% of butanediol, 2-5% of glycerol, 0.05-0.3% of xanthan gum, 0.01-0.1% of sodium hyaluronate, 0.01-0.1% of sodium polyglutamate, 0.03-0.1% of EDTA-2Na, 0.05-0.3% of allantoin, 0.01-0.2% of dipotassium glycyrrhizinate, 1-3% of betaine, 0.1-1% of trehalose, 0.15-0.3% of methyl hydroxybenzoate, 0.01-0.2% of hydrogenated lecithin, 1-3% of PEG-20 methyl glucose sesquistearate, 1-3% of methyl glucose sesquistearate, 1-4% of caprylic/capric triglyceride, 1-5% of polydimethylsiloxane, 0.1-0.5% of tocopherol acetate, 0.05-0.5% of propyl hydroxybenzoate, 0.5-3% of squalane, 0.5-3% of cetyl stearyl alcohol, 1 to 3 percent of jojoba seed oil, 2 to 10 percent of cyclopentadidimethyl siloxane, 0.1 to 0.5 percent of tetrahydroxypropyl ethylenediamine, 0.2 to 1 percent of mixed solution of phenoxyethanol and ethylhexyl glycerol, 1 to 3 percent of hydroxyethyl piperazine ethanesulfonic acid, 1 to 3 percent of nicotinamide, 0.1 to 1 percent of panthenol, 0.01 to 0.1 percent of essence, 4 to 6 percent of the water extract of the cells of the Changbai mountain wild ginseng, and the balance of water.
Preferably, the anti-aging cream comprises the following components in percentage by weight: 0.05 to 0.3 percent of acryloyl dimethyl ammonium taurate/VP copolymer, 2 to 6 percent of butanediol, 2 to 5 percent of glycerol, 0.05 to 0.3 percent of xanthan gum, 0.01 to 0.1 percent of sodium hyaluronate, 0.01 to 0.1 percent of sodium polyglutamate, 0.03 to 0.1 percent of EDTA-2Na, 0.05 to 0.3 percent of allantoin, 0.01 to 0.2 percent of dipotassium glycyrrhizinate, 1 to 3 percent of betaine, 0.1 to 1 percent of trehalose, 0.15 to 0.3 percent of methyl hydroxybenzoate, 0.01 to 0.2 percent of hydrogenated lecithin, 201 to 3 percent of polysorbate, 0.1 to 1 percent of potassium cetyl phosphate, 1 to 4 percent of caprylic/capric triglyceride, 1 to 5 percent of polydimethylsiloxane, 0.1 to 0.5 percent of tocopherol acetate, 0.05 to 0.5 percent of propyl hydroxybenzoate, 0.5 to 3 percent of squalane, 0.5 to 3 percent of cetylstearyl alcohol, 1 to 3 percent of jojoba seed oil, 1 to 3 percent of cyclopentadimethylsiloxane, 0.1 to 0.5 percent of tetrahydroxypropyl ethylenediamine, 0.2 to 1 percent of mixed solution of phenoxyethanol and ethylhexyl glycerol, 1 to 3 percent of hydroxyethyl piperazine ethanesulfonic acid, 1 to 3 percent of nicotinamide, 0.1 to 1 percent of panthenol, 0.01 to 0.1 percent of essence, 4 to 6 percent of the water extract of the Changbai mountain wild ginseng cells and the balance of water.
Preferably, the anti-aging cream comprises the following components in percentage by weight: 0.05-0.3% of acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer, 2-6% of butanediol, 2-5% of glycerol, 0.05-0.3% of ammonium acryloyldimethyl taurate/VP copolymer, 0.01-0.1% of sodium hyaluronate, 0.01-0.1% of sodium polyglutamate, 0.03-0.1% of EDTA-2Na, 0.05-0.3% of allantoin, 0.01-0.2% of dipotassium glycyrrhizinate, 1-3% of betaine, 0.1-1% of trehalose, 0.15-0.3% of methyl hydroxybenzoate, 0.01-0.2% of hydrogenated lecithin, 201-3% of polysorbate, 1-3% of PEG-20 methyl glucose sesquistearate, 0.1-1% of polyglycerol-3-methyl glucose distearate, 1-4% of caprylic/capric triglyceride, 1-5% of polydimethylsiloxane, 0.1-0.5% of tocopherol acetate, 0.05-0.5% of propyl hydroxybenzoate, 0.5-3% of squalane, 0.5-3% of cetyl stearate, 1-3% of jojoba seed oil, 2-10% of cyclopentadimethyl siloxane, 0.1-0.5% of tetrahydroxypropyl ethylenediamine, 0.2-1% of a mixed solution of phenoxyethanol and ethylhexyl glycerol, 1-3% of hydroxyethyl piperazine ethanesulfonic acid, 1-3% of nicotinamide, 0.1-1% of panthenol, 0.01-0.1% of essence, 4-6% of a water extract of a Changbai mountain wild ginseng cell, and the balance of water.
Preferably, the preparation method of the water extract of the wild ginseng in Changbai mountain comprises the following steps:
A. under aseptic conditions, after callus induction, subculture and screening of isolated plant organs, tissues, cells or protoplasts of the Changbai mountain wild ginseng, inoculating the isolated plant organs, tissues, cells or protoplasts on a B5 or MS plant tissue culture medium, and culturing under the culture condition of 25 +/-5 ℃ to obtain a Changbai mountain wild ginseng cell culture;
B. b, washing the Changbai mountain wild ginseng cells obtained in the step A by using pure water;
C. placing the cleaned Changbai mountain wild ginseng cells into a homogenizer, adding pure water, and homogenizing, wherein the weight ratio of the Changbai mountain wild ginseng cells to the water is 1:1-1: 10;
D. filtering the homogenate obtained in step C with a filter membrane with pore diameter of 0.22um, subpackaging, and storing at-5 deg.C.
Preferably, in step B, the washing times are 3 times, and the amount of pure water used in each time is 5 times of the weight of the cells of the Changbai mountain wild ginseng.
Preferably, the callus induction, subculture and screening in the step a specifically comprises the following steps:
1) taking Changbai mountain wild ginseng root as an explant, removing surface epidermis after disinfection, inoculating on a callus induction culture medium, and culturing at 25 +/-2 ℃ to obtain wild ginseng callus;
2) inoculating the callus of wild ginseng obtained in the step 1) into a wild ginseng cell culture medium, culturing at 25 +/-2 ℃, and transferring once in 30-40 days;
3) continuously subculturing the wild ginseng cells obtained in the step 2), and screening by using the growth speed and the wild ginseng saponin content as indexes to obtain a wild ginseng cell line with better growth vigor and highest wild ginseng saponin content.
The invention also provides a preparation method of the anti-aging cream containing the Changbai mountain wild ginseng cell water extract, which mainly comprises the following steps:
(1) adding pure water into a water phase pot, starting rapid stirring, slowly adding acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer or acryloyl dimethyl ammonium taurate/VP copolymer, (butanediol, glycerol, xanthan gum or acryloyl dimethyl ammonium taurate/VP copolymer, sodium hyaluronate and sodium polyglutamate, pre-mixing) into the mixture, fully stirring the mixture to fully disperse and swell the mixture, adding trehalose, allantoin, EDTA-2Na, dipotassium glycyrrhizinate, betaine and methyl hydroxybenzoate, and supplementing 1.5% of pure water;
(2) heating to raise the temperature (target 85 deg.C, deviation + -3 deg.C), adding hydrogenated lecithin, and stirring for 10 min;
(3) sequentially adding PEG-20 methyl glucose sesquistearate or/and polysorbate-20, methyl glucose sesquistearate or potassium cetyl phosphate or polyglycerol-3-methyl glucose distearate, squalane, jojoba seed oil, caprylic/capric triglyceride, polydimethylsiloxane, tocopheryl acetate, cetyl stearyl alcohol and propyl hydroxybenzoate into an oil pan, heating to 85 deg.C, and keeping the temperature for 10 min;
(4) preheating a vacuum emulsifying pot to 40 ℃, vacuumizing, pumping a water phase into the emulsifying pot, starting rapid stirring, starting homogenizing at 1800rpm for 3min, pumping an oil phase, starting homogenizing at 3000rpm for 5min, keeping the temperature and stirring for 15min, then starting cooling, and turning to the next operation;
(5) cooling to 75 deg.C in a vacuum emulsifying pot, adding cyclopentadimethylsiloxane, homogenizing at 3000rpm for 5min, stirring for 10min, and cooling;
(6) cooling to 50 deg.C in a vacuum emulsifying pot, adding the raw materials (tetrahydroxypropyl ethylenediamine, water, pre-dissolved) into the vacuum emulsifying pot, continuously stirring and cooling, cooling to 45 deg.C, adding phenoxyethanol, ethylhexyl glycerol, essence, (hydroxyethyl piperazine ethanesulfonic acid, water, pre-dissolved), (nicotinamide, water, pre-dissolved), (panthenol, water, pre-dissolved), and Changbai mountain wild ginseng cell water extract into the vacuum emulsifying pot one by one, homogenizing at 1800rpm, continuously stirring for 1min and cooling; cooling to 38 deg.C, and discharging.
Compared with the prior art, the invention has the following beneficial effects:
1. the anti-aging cream containing the wild ginseng cell water extract can effectively ensure the activity of the wild ginseng cell water extract, can play a role in reducing wrinkles by matching with various skin care ingredients, and has the effects of moisturizing, brightening and moistening without stimulation.
2. The method adopts the combination of homogenization and ultrafiltration to extract the wild ginseng cell water extract, ensures the extraction sufficiency by combining the two, can obtain the extract with proper grain diameter, does not need evaporation and drying, and has the advantages of simple operation, high extraction efficiency, high yield of active ingredients, energy conservation and improvement on industrial production efficiency compared with the common extraction method.
Detailed Description
The present invention is described in detail below with reference to examples, but the embodiments of the present invention are not limited thereto, and it is obvious that the examples in the following description are only some examples of the present invention, and it is obvious for those skilled in the art to obtain other similar examples without inventive exercise and falling into the scope of the present invention.
Example 1 obtaining of wild ginseng cells
1. Preparation of culture Medium
(1) Preparing wild ginseng callus induction culture medium, and adding 0.2mg/L-0.5mg/LGA (gibberellin), 20g/L-30g/L sucrose and 0.6g/L-0.8g/L agar into MS basal culture medium (formula shown in table 1).
(2) Preparing a wild ginseng cell culture medium, and adding 0.2mg/L-0.5 mg/L6-BA (6-benzylpurine), 2-4mg/L NAA (naphthylacetic acid), 0.6g/L-0.8g/L agar and 20g/L-30g/L cane sugar into an MS basal culture medium (the formula is shown in table 1).
TABLE 1 MS basal Medium
2. Induction of wild ginseng cells
(1) Preparing wild ginseng callus tissues: taking wild ginseng root as an explant, removing surface epidermis after disinfection, inoculating on a callus induction culture medium, and culturing at 25 +/-2 ℃ to obtain callus;
(2) culturing wild ginseng cells: inoculating the wild ginseng callus obtained in the step (1) into a wild ginseng cell culture medium, culturing at 25 +/-2 ℃, and transferring once in 30-40 days;
(3) subculturing and screening wild ginseng cells: continuously subculturing the cells in the step (2), and screening to obtain three wild ginseng cell tissue lines: white, green and yellow, and detecting the growth speed and the content of wild ginseng saponin to determine that the yellow is a cell line with better growth vigor and the highest wild ginseng saponin content.
3. Growth rate determination
(1) And (3) dry weight determination: culturing under the same inoculation and culture conditions, drying the collected culture at 50 deg.C to constant weight under the same culture days, measuring dry weight of each bottle of culture,
4. determination of content
Chromatographic conditions and system applicability test: octadecylsilane chemically bonded silica is used as a filling agent; taking acetonitrile as a mobile phase A and water as a mobile phase B, and carrying out gradient elution according to an elution procedure in the table 2; the detection wavelength was 203 nm. The number of theoretical plates is not less than 6000 according to the peak calculation of ginsenoside Rg 1.
TABLE 2 elution parameters of liquid chromatography
| Time (minutes) | Mobile phase A (%) | Mobile phase B (%) |
| 0～35 | 19 | 81 |
| 35～55 | 19-29 | 81-71 |
| 55～70 | 29 | 71 |
| 70～100 | 29-40 | 71-60 |
Preparation of control solutions: accurately weighing ginsenoside Rg reference substance, ginsenoside Re reference substance and ginsenoside Rb reference substance, adding methanol to obtain mixed solution containing 0.2mg of ginsenoside Re and ginsenoside Rb per 1ml, and shaking.
Preparation of a test solution: taking about 1g of cultured wild ginseng cell powder (screened by a sieve with the number four), precisely weighing, placing in a Soxhlet extractor, adding trichloromethane, heating and refluxing for 3 hours, discarding trichloromethane liquid, volatilizing solvent from dregs, transferring into a 100ml conical flask together with a filter paper cylinder, precisely adding water to saturate n-butyl alcohol by 50ml, sealing, standing overnight, carrying out ultrasonic treatment (power 250W and frequency 50kHz) for 30 minutes, filtering, discarding primary filtrate, precisely measuring 25ml of secondary filtrate, placing in an evaporating dish, evaporating to dryness, dissolving residues with methanol, transferring into a 5ml measuring flask, adding methanol to dilute to scale, shaking uniformly, filtering, and taking secondary filtrate to obtain the final product.
The determination process comprises the following steps: precisely sucking 10 μ l of the reference solution and 10-20 μ l of the test solution, respectively, injecting into a liquid chromatograph, and measuring.
5. Mass propagation of wild ginseng cells
And (4) inoculating the yellow cell line with the highest content in the step (3) into a wild ginseng cell culture medium, culturing at 25 +/-2 ℃, and collecting after 30-40 days to obtain the required culture.
6. Content of effective components in culture
The content of ginsenoside in wild ginseng cells obtained by mass propagation was determined by reference to the above content determination method, and the specific results are shown in Table 3.
TABLE 3 ginsenoside content of massively propagated wild ginseng cells
| RS-0927-1 | RS-927-2 | RS-0928-3 | RS-200915 | RS-0916 | |
| Ginsenoside Rg1 | 0.878％ | 0.784％ | 0.058％ | 0.049％ | 0.035％ |
| Ginsenoside Re | 0.152％ | 0.675％ | 0.197％ | 0.285％ | 0.330％ |
| Ginsenoside Rg2 | 0.066％ | 0.185％ | 0.022％ | 0.023％ | 0.025％ |
| Ginsenoside Rb1 | 0.170％ | 0.541％ | 1.039％ | 1.149％ | 1.628％ |
| Ginsenoside Rc | 0.025％ | 0.079％ | 0.415％ | 0.426％ | 0.843％ |
| Ginsenoside Rb2 | 0.032％ | 0.109％ | 0.427％ | 0.404％ | 0.761％ |
| Ginsenoside Rd | 0.042％ | 0.189％ | 0.301％ | 0.242％ | 0.598％ |
| Total saponin content | 1.364％ | 2.562％ | 2.458％ | 2.577％ | 4.221％ |
Example 2
A method for preparing anti-aging cream containing water extract of Changbai mountain wild ginseng cell comprises the following steps:
(1) adding pure water into water phase pot, rapidly stirring, and adding acrylic acid (ester)/C10-30Adding alkanol acrylate cross-linked polymer, (butanediol, glycerol, xanthan gum, sodium hyaluronate and sodium polyglutamate, premixing) slowly, stirring to fully disperse and swell, adding trehalose, allantoin, EDTA-2Na, dipotassium glycyrrhizinate, betaine and methyl hydroxybenzoate, and adding pure water 1.5%;
(2) heating to raise the temperature (target 85 deg.C, deviation + -3 deg.C), adding hydrogenated lecithin, and stirring for 10 min; simultaneously carrying out oil phase pot operation:
(3) sequentially adding PEG-20 methyl glucose sesquistearate, squalane, jojoba seed oil, caprylic/capric triglyceride, polydimethylsiloxane, tocopheryl acetate, cetearyl alcohol and methyl hydroxybenzoate into an oil pan, heating to 85 deg.C, and keeping the temperature for 10 min;
(4) preheating a vacuum emulsifying pot to 40 ℃, vacuumizing, pumping a water phase into the emulsifying pot, starting rapid stirring, starting homogenizing at 1800rpm for 3min, pumping an oil phase, starting homogenizing at 3000rpm for 5min, keeping the temperature and stirring for 15min, then starting cooling, and turning to the next operation;
(5) cooling to 75 deg.C in a vacuum emulsifying pot, adding cyclopentadimethylsiloxane, homogenizing at 3000rpm for 5min, stirring for 10min, and cooling;
(6) cooling to 50 deg.C in a vacuum emulsifying pot, adding the raw materials (tetrahydroxypropyl ethylenediamine, water, pre-dissolved) into the vacuum emulsifying pot, continuously stirring and cooling, cooling to 45 deg.C, adding phenoxyethanol, ethylhexyl glycerol, essence, (hydroxyethyl piperazine ethanesulfonic acid, water, pre-dissolved), (nicotinamide, water, pre-dissolved), (panthenol, water, pre-dissolved), and Changbai mountain wild ginseng cell water extract into the vacuum emulsifying pot one by one, homogenizing at 1800rpm, continuously stirring for 1min and cooling; cooling to 38 deg.C, and discharging.
The preparation method of the Changbai mountain wild ginseng cell water extract comprises the following steps:
a, taking fresh Changbai mountain wild ginseng cells, and washing with pure water;
b, putting the washed Changbai mountain wild ginseng cells into a homogenizer, adding pure water, and homogenizing, wherein the weight ratio of the Changbai mountain wild ginseng cells to the water is 1: 1;
c, filtering the homogenate obtained in the step B by using a filter membrane with the aperture of 0.22um, subpackaging and storing at-5 ℃.
Wherein, the water extract of the Changbai mountain wild ginseng cell comprises the following components by weight percent: ginsenoside Rg 10.0211%, ginsenoside Re 0.0151%, ginsenoside Rg 20.0038%, ginsenoside Rb10.0023%, ginsenoside Rc 0.0001%, ginsenoside Rb20.0003% and ginsenoside Rd 0.0006%.
Table 4: anti-aging cream component composition of example 2
Example 3
A method for preparing anti-aging cream containing water extract of Changbai mountain wild ginseng cell comprises the following steps:
(1) adding pure water into a water phase pot, starting rapid stirring, slowly adding acryloyl dimethyl ammonium taurate/VP copolymer, (butanediol, glycerol, xanthan gum, sodium hyaluronate and sodium polyglutamate, and mixing in advance), fully stirring to fully disperse and swell, adding trehalose, allantoin, EDTA-2Na, dipotassium glycyrrhizinate, betaine and methyl hydroxybenzoate, and supplementing 1.5% of pure water;
(2) heating to raise the temperature (target 85 deg.C, deviation + -3 deg.C), adding hydrogenated lecithin, and stirring for 10 min; simultaneously carrying out oil phase pot operation:
(3) sequentially adding polysorbate-20, potassium cetyl phosphate, squalane, jojoba oil, caprylic/capric triglyceride, polydimethylsiloxane, tocopheryl acetate, cetearyl alcohol and propyl hydroxybenzoate into an oil pan, heating to 85 deg.C, and keeping the temperature for 10 min;
(4) preheating a vacuum emulsifying pot to 40 ℃, vacuumizing, pumping a water phase into the emulsifying pot, starting rapid stirring, starting homogenizing at 1800rpm for 3min, pumping an oil phase, starting homogenizing at 3000rpm for 5min, keeping the temperature and stirring for 15min, then starting cooling, and turning to the next operation;
(5) cooling to 75 deg.C in a vacuum emulsifying pot, adding cyclopentadimethylsiloxane, homogenizing at 3000rpm for 5min, stirring for 10min, and cooling;
(6) cooling to 50 deg.C in a vacuum emulsifying pot, adding the raw materials (tetrahydroxypropyl ethylenediamine, water, pre-dissolved) into the vacuum emulsifying pot, continuously stirring and cooling, cooling to 45 deg.C, adding phenoxyethanol, ethylhexyl glycerol, essence, (hydroxyethyl piperazine ethanesulfonic acid, water, pre-dissolved), (nicotinamide, water, pre-dissolved), (panthenol, water, pre-dissolved), and Changbai mountain wild ginseng cell water extract into the vacuum emulsifying pot one by one, homogenizing at 1800rpm, continuously stirring for 1min and cooling; cooling to 38 deg.C, and discharging.
The preparation method of the Changbai mountain wild ginseng cell water extract comprises the following steps:
a, taking fresh Changbai mountain wild ginseng cells, and washing with pure water;
b, putting the washed Changbai mountain wild ginseng cells into a homogenizer, adding pure water, and homogenizing, wherein the weight ratio of the Changbai mountain wild ginseng cells to the water is 1: 1;
c, filtering the homogenate obtained in the step B by using a filter membrane with the aperture of 0.22um, subpackaging and storing at-5 ℃.
Table 5: anti-aging cream component composition of example 3
Example 4
A method for preparing anti-aging cream containing water extract of Changbai mountain wild ginseng cell comprises the following steps:
(1) adding pure water into water phase pot, rapidly stirring, and adding acrylic acid (ester)/C10-30Adding alkanol acrylate cross-linked polymer, (butanediol, glycerol, ammonium acryloyldimethyl taurate/VP copolymer, sodium hyaluronate and sodium polyglutamate, mixing in advance) slowly, stirring sufficiently to disperse and swell sufficiently, adding trehalose, allantoin, EDTA-2Na, dipotassium glycyrrhizinate, betaine and methyl hydroxybenzoate, and supplementing pure water by 1.5%;
(2) heating to raise the temperature (target 85 deg.C, deviation + -3 deg.C), adding hydrogenated lecithin, and stirring for 10 min; simultaneously carrying out oil phase pot operation:
(3) sequentially adding polysorbate-20, PEG-20 methyl glucose sesquistearate, polyglycerol-3 methyl glucose distearate, squalane, jojoba oil, caprylic/capric triglyceride, polydimethylsiloxane, tocopheryl acetate, cetyl stearyl alcohol and methylparaben into an oil pan, heating to 85 deg.C, and keeping the temperature for 10 min;
(4) preheating a vacuum emulsifying pot to 40 ℃, vacuumizing, pumping a water phase into the emulsifying pot, starting rapid stirring, starting homogenizing at 1800rpm for 3min, pumping an oil phase, starting homogenizing at 3000rpm for 5min, keeping the temperature and stirring for 15min, then starting cooling, and turning to the next operation;
(5) cooling to 75 deg.C in a vacuum emulsifying pot, adding cyclopentadimethylsiloxane, homogenizing at 3000rpm for 5min, stirring for 10min, and cooling;
(6) cooling to 50 deg.C in a vacuum emulsifying pot, adding the raw materials (tetrahydroxypropyl ethylenediamine, water, pre-dissolved) into the vacuum emulsifying pot, continuously stirring and cooling, cooling to 45 deg.C, adding phenoxyethanol, ethylhexyl glycerol, essence, (hydroxyethyl piperazine ethanesulfonic acid, water, pre-dissolved), (nicotinamide, water, pre-dissolved), (panthenol, water, pre-dissolved), and Changbai mountain wild ginseng cell water extract into the vacuum emulsifying pot one by one, homogenizing at 1800rpm, continuously stirring for 1min and cooling; cooling to 38 deg.C, and discharging.
The preparation method of the Changbai mountain wild ginseng cell water extract comprises the following steps:
a, taking fresh Changbai mountain wild ginseng cells, and washing with pure water;
b, putting the washed Changbai mountain wild ginseng cells into a homogenizer, adding pure water, and homogenizing, wherein the weight ratio of the Changbai mountain wild ginseng cells to the water is 1: 1;
c, filtering the homogenate obtained in the step B by using a filter membrane with the aperture of 0.22um, subpackaging and storing at-5 ℃.
Table 6: component composition of anti-aging cream of example 4
Comparative example 1
A preparation method of anti-aging cream comprises the following steps:
(1) adding pure water into water phase pot, rapidly stirring, and adding acrylic acid (ester)/C10-30Adding alkanol acrylate cross-linked polymer, (butanediol, glycerol, xanthan gum, sodium hyaluronate and sodium polyglutamate, premixing) slowly, stirring to fully disperse and swell, adding trehalose, allantoin, EDTA-2Na, dipotassium glycyrrhizinate, betaine and methyl hydroxybenzoate, and adding pure water 1.5%;
(2) heating to raise the temperature (target 85 deg.C, deviation + -3 deg.C), adding hydrogenated lecithin, and stirring for 10 min; simultaneously carrying out oil phase pot operation:
(3) sequentially adding PEG-20 methyl glucose sesquistearate, squalane, jojoba seed oil, caprylic/capric triglyceride, polydimethylsiloxane, tocopheryl acetate, cetearyl alcohol and methyl hydroxybenzoate into an oil pan, heating to 85 deg.C, and keeping the temperature for 10 min;
(4) preheating a vacuum emulsifying pot to 40 ℃, vacuumizing, pumping a water phase into the emulsifying pot, starting rapid stirring, starting homogenizing at 1800rpm for 3min, pumping an oil phase, starting homogenizing at 3000rpm for 5min, keeping the temperature and stirring for 15min, then starting cooling, and turning to the next operation;
(5) cooling to 75 deg.C in a vacuum emulsifying pot, adding cyclopentadimethylsiloxane, homogenizing at 3000rpm for 5min, stirring for 10min, and cooling;
(6) cooling to 50 deg.C, adding the raw materials (tetrahydroxypropyl ethylenediamine, water, pre-dissolved) into vacuum emulsifying pan, continuously stirring, cooling to 45 deg.C, adding phenoxyethanol, ethylhexyl glycerol, essence, (hydroxyethyl piperazine ethanesulfonic acid, water, pre-dissolved), (nicotinamide, water, pre-dissolved), (panthenol, water, pre-dissolved), and the balance water into vacuum emulsifying pan, homogenizing at 1800rpm, stirring for 1min, and cooling; cooling to 38 deg.C, and discharging.
Table 7: composition of anti-aging cream of comparative example 1
Example 5
Evaluation of anti-aging cream Effect
The test method comprises the following steps: in the same area, 200 female volunteers with aged skin were screened at the same time, aged 35-50 years, and the average age was 43 years.
And (3) inclusion standard: the skin around the eyes has mild or moderate wrinkles, dry skin, reduced elasticity, wrinkles, pigmentation, dull skin color and other skin aging phenomena; and randomly dividing the eye-care product into 4 groups, wherein 50 persons in each group and 4 groups of volunteers respectively correspond to the anti-aging creams in the embodiments 2-4 and the comparative example 1, and each group of volunteers uses the corresponding test product as a unique eye-care product, after cleaning the face in the morning and in the evening, the eye-care product is smeared around the eyes and is gently pressed until the eye-care product is absorbed, the test time is 90 days, the volunteers participating in the test feed back the change condition and the use feeling of the eye skin every day, the condition is recorded, the data is summarized, and the effect evaluation is carried out on the test subjects.
Skin aging evaluation: evaluated by observing the subject's test area (crow's feet, glabellar lines, forehead wrinkles).
And (4) judging the standard:
has no wrinkles
Mild: 2-3 wrinkles, length less than 1cm
Medium: 3-6 wrinkles, length less than 2cm
And (3) severe degree: has several wrinkles of length more than 3cm and simultaneously has shallow wrinkles
Table 8: statistics of skin aging of each group of subjects after completion of the test
| Group of | Without wrinkles (example) | Slight (example) | Moderate (example) | Severe (example) |
| Example 2 | 44 | 5 | 1 | 0 |
| Example 3 | 40 | 7 | 3 | 0 |
| Example 4 | 41 | 4 | 5 | 0 |
| Comparative example 1 | 19 | 20 | 5 | 6 |
The tests show that the anti-aging cream prepared in the embodiments 2 to 4 can relieve and prevent wrinkles, and has a good moisturizing effect, so that the anti-aging purpose is achieved.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.
Claims (9)
1. An anti-aging cream containing a Changbai mountain wild ginseng cell water extract is characterized by comprising the following components in parts by weight:
50-75 parts of pure water, 10-25 parts of humectant, 0.2-0.8 part of thickener, 0.05-0.4 part of chelating agent, 8-20 parts of emollient, 3-6 parts of emulsifier, 1-10 parts of skin conditioner, 0.1-10 parts of Changbai mountain wild ginseng cell water extract, 0.02-0.4 part of essence and 0.1-0.8 part of preservative.
2. The anti-aging cream as claimed in claim 1, wherein the humectant is one or a combination of two or more selected from glycerin, propylene glycol, butylene glycol, 1, 2-hexanediol, polyglutamic acid, sodium hyaluronate, trehalose, and betaine; the thickening agent is one or a composition of more than two of xanthan gum, acrylic acid/C10-30 alkanol acrylate cross-linked polymer, acryloyl dimethyl ammonium taurate/VP copolymer and cetearyl alcohol; the chelating agent is one or two of EDTA-2Na and tetrahydroxypropyl ethylenediamine; the emollient is one or more of squalane, jojoba oil, caprylic/capric triglyceride, polydimethylsiloxane, tocopherol acetate and cyclopentadimethylsiloxane; the emulsifier is one or more of hydrogenated lecithin, potassium cetyl phosphate, PEG-20 methyl glucose sesquistearate, polysorbate-20, and polyglycerol-3-methyl glucose distearate; the skin conditioning agent is one or more of panthenol, nicotinamide, dipotassium glycyrrhizinate, allantoin and hydroxyethyl piperazine ethane sulfonic acid; the essence is one or a composition of more than two of flower fragrance, fruit fragrance, green fragrance and costustoot; the preservative is one or a composition of more than two of methyl hydroxybenzoate, propyl hydroxybenzoate, phenoxyethanol and ethylhexylglycerin.
3. The anti-aging cream as claimed in claim 1, wherein the anti-aging cream comprises the following components in percentage by weight: 0.05-0.3% of acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer, 2-6% of butanediol, 2-5% of glycerol, 0.05-0.3% of xanthan gum, 0.01-0.1% of sodium hyaluronate, 0.01-0.1% of sodium polyglutamate, 0.03-0.1% of EDTA-2Na, 0.05-0.3% of allantoin, 0.01-0.2% of dipotassium glycyrrhizinate, 1-3% of betaine, 0.1-1% of trehalose, 0.15-0.3% of methyl hydroxybenzoate, 0.01-0.2% of hydrogenated lecithin, 1-3% of PEG-20 methyl glucose sesquistearate, 1-3% of methyl glucose sesquistearate, 1-4% of caprylic/capric triglyceride, 1-5% of polydimethylsiloxane, 0.1-0.5% of tocopherol acetate, 0.05-0.5% of propyl hydroxybenzoate, 0.5-3% of squalane, 0.5-3% of cetyl stearyl alcohol, 1 to 3 percent of jojoba seed oil, 2 to 10 percent of cyclopentadidimethyl siloxane, 0.1 to 0.5 percent of tetrahydroxypropyl ethylenediamine, 0.2 to 1 percent of mixed solution of phenoxyethanol and ethylhexyl glycerol, 1 to 3 percent of hydroxyethyl piperazine ethanesulfonic acid, 1 to 3 percent of nicotinamide, 0.1 to 1 percent of panthenol, 0.01 to 0.1 percent of essence, 4 to 6 percent of the water extract of the cells of the Changbai mountain wild ginseng, and the balance of water.
4. The anti-aging cream as claimed in claim 1, wherein the anti-aging cream comprises the following components in percentage by weight: 0.05 to 0.3 percent of acryloyl dimethyl ammonium taurate/VP copolymer, 2 to 6 percent of butanediol, 2 to 5 percent of glycerol, 0.05 to 0.3 percent of xanthan gum, 0.01 to 0.1 percent of sodium hyaluronate, 0.01 to 0.1 percent of sodium polyglutamate, 0.03 to 0.1 percent of EDTA-2Na, 0.05 to 0.3 percent of allantoin, 0.01 to 0.2 percent of dipotassium glycyrrhizinate, 1 to 3 percent of betaine, 0.1 to 1 percent of trehalose, 0.15 to 0.3 percent of methyl hydroxybenzoate, 0.01 to 0.2 percent of hydrogenated lecithin, 201 to 3 percent of polysorbate, 0.1 to 1 percent of potassium cetyl phosphate, 1 to 4 percent of caprylic/capric triglyceride, 1 to 5 percent of polydimethylsiloxane, 0.1 to 0.5 percent of tocopherol acetate, 0.05 to 0.5 percent of propyl hydroxybenzoate, 0.5 to 3 percent of squalane, 0.5 to 3 percent of cetylstearyl alcohol, 1 to 3 percent of jojoba seed oil, 1 to 3 percent of cyclopentadimethylsiloxane, 0.1 to 0.5 percent of tetrahydroxypropyl ethylenediamine, 0.2 to 1 percent of mixed solution of phenoxyethanol and ethylhexyl glycerol, 1 to 3 percent of hydroxyethyl piperazine ethanesulfonic acid, 1 to 3 percent of nicotinamide, 0.1 to 1 percent of panthenol, 0.01 to 0.1 percent of essence, 4 to 6 percent of the water extract of the Changbai mountain wild ginseng cells and the balance of water.
5. The anti-aging cream as claimed in claim 1, wherein the anti-aging cream comprises the following components in percentage by weight: acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer 0.05-0.3%, butanediol 2-6%, glycerol 2-5%, ammonium acryloyldimethyl taurate/VP copolymer 0.05-0.3%, sodium hyaluronate 0.01-0.1%, sodium polyglutamate 0.01-0.1%, EDTA-2Na 0.03-0.1%, allantoin 0.05-0.3%, dipotassium glycyrrhizinate 0.01-0.2%, betaine 1-3%, trehalose 0.1-1%, methylparaben 0.15-0.3%, hydrogenated lecithin 0.01-0.2%, polysorbate-201-3%, PEG-20 methyl glucose sesquistearate 1-3%, polyglycerol-3-methyl glucose distearate 0.1-1%, caprylic acid/capric acid triglyceride 1-4%, polydimethylsiloxane 1-5%, tocopherol acetate 0.1-0.5%, 0.05-0.5% of propyl hydroxybenzoate, 0.5-3% of squalane, 0.5-3% of cetyl stearate alcohol, 1-3% of jojoba seed oil, 2-10% of cyclopentadimethylsiloxane, 0.1-0.5% of tetrahydroxypropylethylenediamine, 0.2-1% of a mixed solution of phenoxyethanol and ethylhexyl glycerol, 1-3% of hydroxyethyl piperazine ethanesulfonic acid, 1-3% of nicotinamide, 0.1-1% of panthenol, 0.01-0.1% of essence, 4-6% of a water extract of Changbai mountain wild ginseng cells and the balance of water.
6. The anti-aging cream as claimed in any one of claims 1 to 5, wherein the preparation method of the aqueous extract of Changbai mountain wild ginseng cell comprises the following steps:
A. under aseptic conditions, after callus induction, subculture and screening of isolated plant organs, tissues, cells or protoplasts of the Changbai mountain wild ginseng, inoculating the isolated plant organs, tissues, cells or protoplasts on a plant tissue culture medium, and culturing under the culture condition of 25 +/-5 ℃ to obtain a Changbai mountain wild ginseng cell culture;
B. b, washing the Changbai mountain wild ginseng cells obtained in the step A by using pure water;
C. placing the cleaned Changbai mountain wild ginseng cells into a homogenizer, adding pure water, and homogenizing, wherein the weight ratio of the Changbai mountain wild ginseng cells to the water is 1:1-1: 10;
D. filtering the homogenate obtained in step C with a filter membrane with pore diameter of 0.22um, subpackaging, and storing at-5 deg.C.
7. The anti-aging cream as claimed in claim 6, wherein the callus induction, subculture and screening in step A are carried out by the following specific processes:
1) taking Changbai mountain wild ginseng roots, sterilizing, removing surface epidermis, inoculating on a callus induction culture medium, and culturing at 25 +/-2 ℃ to obtain wild ginseng callus;
2) inoculating the callus of wild ginseng obtained in the step 1) into a wild ginseng cell culture medium, culturing at 25 +/-2 ℃, and transferring once in 30-40 days;
3) continuously subculturing the wild ginseng cells obtained in the step 2), and screening by using the growth speed and the wild ginseng saponin content as indexes to obtain a wild ginseng cell line with better growth vigor and highest wild ginseng saponin content.
8. The method for preparing the anti-aging cream as claimed in any one of claims 3 to 7, which is characterized by mainly comprising the following steps:
(1) adding pure water into a water phase pot, starting stirring, adding acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer or acryloyl dimethyl ammonium taurate/VP copolymer, butanediol, glycerol, xanthan gum or acryloyl dimethyl ammonium taurate/VP copolymer, sodium hyaluronate and sodium polyglutamate, fully dispersing and swelling, and adding trehalose, allantoin, EDTA-2Na, dipotassium glycyrrhizinate, betaine and methyl hydroxybenzoate;
(2) heating the water phase pot to 80-90 ℃, adding hydrogenated lecithin, and stirring for 5-30 min under heat preservation;
(3) sequentially adding PEG-20 methyl glucose sesquistearate or/and polysorbate-20, methyl glucose sesquistearate or potassium cetyl phosphate or polyglycerol-3-methyl glucose distearate, squalane, jojoba seed oil, caprylic/capric triglyceride, polydimethylsiloxane, tocopheryl acetate, cetyl stearyl alcohol and propyl hydroxybenzoate into an oil pan, heating to 80-90 ℃, and keeping the temperature for 10-30 min;
(4) preheating a vacuum emulsification pot to 35-45 ℃, pumping the water phase obtained in the step (2) into the vacuum emulsification pot, starting stirring, starting homogenizing at 1500-2000 rpm for 2-30 min, pumping the oil phase obtained in the step (3), starting homogenizing at 2500-3500 rpm for 5-30 min, and keeping the temperature and stirring for 10-30 min;
(5) cooling the vacuum emulsification pot to 65-75 ℃, adding cyclopentadimethylsiloxane, homogenizing at 2500-3500 rpm for 3-10 min, and stirring for 10-20 min under the condition of heat preservation;
(6) cooling a vacuum emulsification pot to 48-52 ℃, adding a tetrahydroxypropyl ethylenediamine aqueous solution, stirring and cooling to 42-45 ℃, adding phenoxyethanol, ethylhexyl glycerol, essence, a hydroxyethyl piperazine ethanesulfonic acid aqueous solution, a nicotinamide aqueous solution, a panthenol aqueous solution and a Changbai mountain wild ginseng cell water extract into the vacuum emulsification pot one by one, homogenizing at 1500-2000 rpm for 1-10 min, stirring and cooling to below 38 ℃, and discharging to obtain the feed.
9. The method of claim 8, wherein the butylene glycol, glycerin, xanthan gum or acryloyldimethyl taurate/VP copolymer, sodium hyaluronate and sodium polyglutamate in step (1) are mixed in advance and slowly added to the aqueous phase pot.
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