CN110251561B - Antiviral compound traditional Chinese medicine preparation for pigs and preparation method thereof - Google Patents
Antiviral compound traditional Chinese medicine preparation for pigs and preparation method thereof Download PDFInfo
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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Abstract
The invention discloses an antiviral compound traditional Chinese medicine preparation for pigs and a preparation method thereof. The compound traditional Chinese medicine preparation is prepared from 2-5 parts of mulberry leaves, 2-5 parts of spanishneedles herb, 1-2 parts of liquorice, 1-5 parts of gynostemma pentaphylla and 5-10 parts of soybean meal, composite bacteria consisting of bacillus subtilis, bacillus licheniformis, lactobacillus and saccharomycetes, and composite enzyme consisting of neutral protease and acid protease, wherein the traditional Chinese medicine preparation is prepared by mixing and fermenting the traditional Chinese medicine composition, the composite bacteria and the composite enzyme. The traditional Chinese medicine preparation is added into the feed to feed pigs, so that the infection and incidence ratio of the virus diseases of the swinery can be reduced, the death rate of the pigs suffering from the diseases can be controlled, the production performance of the pigs and the vaccine immunity effect can be improved, the diarrhea rate can be reduced, and the like.
Description
Technical Field
The invention belongs to the technical field of antiviral diseases in the breeding industry, and particularly relates to an antiviral compound traditional Chinese medicine preparation for pigs and a preparation method thereof.
Background
In the pig industry, when suffering from virus infection or invasion of swine fever, porcine pseudorabies, porcine reproductive and respiratory syndrome, porcine circovirus and other virus diseases, the pig is easy to cause higher mortality rate of pigs and even death of all pigs. At present, the vaccine is mainly controlled through vaccine immunity prevention, the high immune serum can be injected during the disease occurrence, the death rate is controlled through emergency immunity, the pigs can be healed or endured and possibly carry toxin for life, the immune function is reduced, and the economic value is lost.
In addition, the high immune serum is expensive, the utilization rate of the pig farm is low, and the epidemic diseases are mainly prevented by means of vaccine immunization, but some diseases such as pig breeding and respiratory syndrome, pig ring disease and the like can cause immunosuppression so that pig groups are infected with other diseases or have immune failure and the like; or some viral diseases have different serotypes, genotypes, etc., while the injected vaccine may not have cross-protection between different serotypes, genotypes; during the evolution process, the virus may have variation to cause virulence enhancement, and may cause poor vaccine immunity effect or even immunity failure.
In the aspect of treatment, the conventional method is to prevent secondary bacterial infection by symptomatic treatment, such as high fever symptoms caused by virus infection, by adopting antipyretic analgesic drugs such as intramuscular analgin, aminopyrine and the like and combining antibiotics, wherein the drugs only have the effects of simply relieving fever, resisting inflammation and easing pain, have no antiviral effect, only can be controlled temporarily and are easy to relapse, and the death rate can be reduced to a certain extent, but the growth performance of pigs is greatly influenced, and the economic benefit is reduced.
Many Chinese herbal medicines have natural antibacterial and antiviral effects and can regulate animal organism immunity, and different from western medicines, the antibacterial and antiviral effects of the Chinese herbal medicines are multi-target, and are not easy to generate drug resistance, virus and bacteria variation and the like; meanwhile, the Chinese herbal medicines are used for preventing and treating animal diseases generally without side effects and drug residues, and the requirements of 'food safety', 'no-resistance cultivation', 'animal welfare' and the like are met. Therefore, the development of the antiviral compound traditional Chinese medicine preparation for pigs has important significance.
Disclosure of Invention
The invention aims at providing an antiviral compound traditional Chinese medicine preparation for pigs and a preparation method thereof aiming at several important viral diseases in pig industry.
In order to achieve the above purpose, the invention adopts the following technical scheme:
the antiviral compound traditional Chinese medicine preparation for pigs is prepared from a traditional Chinese medicine composition, compound bacteria and compound enzyme, wherein the traditional Chinese medicine composition comprises the following components in parts by weight: 2-5 parts of mulberry leaf, 2-5 parts of spanishneedles herb, 1-2 parts of liquorice, 1-5 parts of gynostemma pentaphylla and 5-10 parts of soybean meal; the composite bacteria consist of bacillus subtilis, bacillus licheniformis, lactobacillus and saccharomycetes; the complex enzyme consists of neutral protease and acid protease.
Further, in the composite bacteria, the weight ratio of the bacillus subtilis to the traditional Chinese medicine composition is 1:2000-5000, the weight ratio of the bacillus licheniformis to the traditional Chinese medicine composition is 1:2000-5000, the weight ratio of the lactobacillus to the traditional Chinese medicine composition is 1:2000-5000, and the weight ratio of the saccharomycete to the traditional Chinese medicine composition is 1:2000-5000.
Further, in the complex enzyme, the weight ratio of the neutral protease to the traditional Chinese medicine composition is 1:5000-10000, and the weight ratio of the acid protease to the traditional Chinese medicine composition is 1:3000-8000.
The preparation method of the antiviral compound traditional Chinese medicine preparation for pigs comprises the following steps: mixing folium Mori, herba Bidentis Bipinnatae, glycyrrhrizae radix, herba Gynostemmatis and bean pulp, adding bacillus subtilis, bacillus licheniformis, lactobacillus, yeast, neutral protease and acid protease, mixing, adding water, fermenting, and drying the fermentation product to obtain the antivirus compound Chinese medicinal preparation for pig.
Further, the water is added in an amount such that the water content of the total material is 30-40%.
Further, the fermentation condition is that the fermentation is carried out for 2-4d at 35-38 ℃ and after-ripening is carried out for 7-15d at room temperature.
Further, the fermentation product is dried to a moisture content of not more than 13%.
The application method of the antiviral compound traditional Chinese medicine preparation for pigs comprises the following steps: the compound traditional Chinese medicine preparation is directly mixed into pig feed according to the weight ratio of 0.5-2 percent of pig feed.
Animal virus diseases are generally considered to be febrile wasting diseases, and are mostly treated by adopting heat clearing and detoxifying combined with spleen strengthening and deficiency tonifying medicines, and are prevented by using medicines for regulating immunity. The invention relates to a Chinese herbal medicine compound developed aiming at several important viral diseases in pig industry, which is prepared by mixing and fermenting mulberry leaves, spanishneedles herb, liquorice, gynostemma pentaphylla, bean pulp, bacillus subtilis, bacillus licheniformis, lactobacillus, saccharomycetes, neutral protease and acid protease. Wherein, the spanishneedles herb has the functions of resisting bacteria and viruses, resisting inflammation and easing pain, protecting liver, clearing heat, detoxicating, removing blood stasis, reducing swelling and the like, and is commonly used for treating enteritis, diarrhea and other diseases in folks. The mulberry leaf has the functions of clearing heat and detoxicating, and also has the functions of anti-inflammatory, antibacterial and antivirus, and can improve the immunity of organisms. The licorice has the functions of clearing heat and detoxicating, eliminating phlegm and relieving cough, resisting inflammation and allergy, and the like, and is commonly found in the traditional Chinese medicine prescription, and has the function of harmonizing various medicines. The gynostemma pentaphylla has the effects of replenishing qi to invigorate the spleen, resolving phlegm and relieving cough, and clearing heat and detoxicating; the efficacy is classified into deficiency-tonifying drugs, phlegm-resolving drugs and heat-clearing drugs; researches show that gynostemma pentaphylla has a good effect of regulating organism immunity. The soybean isoflavone in the soybean meal can resist oxidation and delay aging, and researches show that the soybean isoflavone can promote the development of immune organs and has the effect of improving the immune function.
The invention has the beneficial effects that: the traditional Chinese medicine preparation provided by the invention is added into feed to feed pigs, so that the infection and incidence ratio of the porcine virus diseases can be reduced, the death rate of the affected pigs can be controlled, the production performance of the porcine raw pigs and the vaccine immunity effect can be improved, the diarrhea rate can be reduced, and the like.
Drawings
FIG. 1 is a graph showing the symptoms of death of mice infected with pseudorabies virus by the pharmaceutical formulation in the compound of example 6;
a is a low dose group, B is a medium dose group, C is a high dose group, D is a PrV control group, and E is a valacyclovir group;
FIG. 2 shows the total serum IgG content (g/L) of piglets after 28d administration of the compound traditional Chinese medicine preparation in example 7; p <0.01 compared to the blank; * P <0.001;
FIG. 3 is a graph showing the diarrhea rate (%) of the piglets of the test period 28d in example 7; p <0.01 compared to the blank;
FIG. 4 is the feed to meat ratio of the piglets of example 7, run 28 d; p <0.05, < P <0.01 compared to the blank.
Detailed Description
The antiviral compound traditional Chinese medicine preparation for pigs is prepared from a traditional Chinese medicine composition, compound bacteria and compound enzyme, wherein the traditional Chinese medicine composition comprises the following components in parts by weight: 2-5 parts of mulberry leaf, 2-5 parts of spanishneedles herb, 1-2 parts of liquorice, 1-5 parts of gynostemma pentaphylla and 5-10 parts of soybean meal; the composite bacteria consist of bacillus subtilis, bacillus licheniformis, lactobacillus and saccharomycetes; the complex enzyme consists of neutral protease and acid protease. Wherein, the weight ratio of bacillus subtilis to the traditional Chinese medicine composition is 1:2000-5000, the weight ratio of bacillus licheniformis to the traditional Chinese medicine composition is 1:2000-5000, the weight ratio of lactobacillus to the traditional Chinese medicine composition is 1:2000-5000, the weight ratio of saccharomycetes to the traditional Chinese medicine composition is 1:2000-5000, the weight ratio of neutral proteinase to the traditional Chinese medicine composition is 1:5000-10000, and the weight ratio of acid proteinase to the traditional Chinese medicine composition is 1:3000-8000.
The preparation method of the antiviral compound traditional Chinese medicine preparation for pigs comprises the following steps: mixing folium Mori, herba Bidentis Bipinnatae, glycyrrhrizae radix, herba Gynostemmatis and bean pulp, adding bacillus subtilis, bacillus licheniformis, lactobacillus, yeast, neutral protease and acid protease, mixing, adding water to control the water content of the total material to 30-40%, fermenting at 35-38deg.C for 2-4d, after-ripening at room temperature for 7-15d, and drying the fermentation product at low temperature until water content is not higher than 13% to obtain the final product.
The application method of the antiviral compound traditional Chinese medicine preparation for pigs comprises the following steps: the compound traditional Chinese medicine preparation is directly mixed into pig feed according to the weight ratio of 0.5-2 percent of pig feed.
Example 1
Antiviral compound traditional Chinese medicine preparation for pigs
1) Raw materials: 2 parts of mulberry leaf, 5 parts of spanishneedles herb, 1 part of liquorice, 5 parts of gynostemma pentaphylla and 5 parts of soybean meal (the total weight of the raw materials is recorded as A);
bacillus subtilis (1:2000 weight ratio to A)
Bacillus licheniformis (1:5000 weight ratio to A)
Lactic acid bacteria (1:2000 weight ratio to A)
Yeast (1:5000 weight ratio to A)
Neutral proteinase (1:5000 weight ratio to A)
Acid proteinase (1:3000 weight ratio to A)
2) The preparation method comprises the following steps: mixing mulberry leaves, spanishneedles herb, liquorice, gynostemma pentaphylla and bean pulp, adding bacillus subtilis, bacillus licheniformis, lactobacillus, saccharomycetes, neutral protease and acid protease, uniformly mixing, adding water to control the water content of the total material to be 30%, fermenting for 4d at 35 ℃, after-ripening for 7d at room temperature, and drying the fermentation product at low temperature until the water content is not higher than 13% after the fermentation is finished, thereby obtaining the antiviral compound traditional Chinese medicine preparation for pigs.
Example 2
Antiviral compound traditional Chinese medicine preparation for pigs
1) Raw materials: 5 parts of mulberry leaf, 2 parts of spanishneedles herb, 2 parts of liquorice, 1 part of gynostemma pentaphylla and 10 parts of soybean meal (the total weight of the raw materials is recorded as A);
bacillus subtilis (1:5000 weight ratio to A)
Bacillus licheniformis (1:2000 weight ratio to A)
Lactic acid bacteria (1:5000 weight ratio to A)
Yeast (1:2000 weight ratio to A)
Neutral proteinase (1:10000 weight ratio to A)
Acid proteinase (1:3000 weight ratio to A)
2) The preparation method comprises the following steps: mixing mulberry leaves, spanishneedles herb, liquorice, gynostemma pentaphylla and bean pulp, adding bacillus subtilis, bacillus licheniformis, lactobacillus, saccharomycetes, neutral protease and acid protease, uniformly mixing, adding water to control the water content of the total material to be 40%, fermenting for 2d at 38 ℃, after-ripening for 15d at room temperature, and drying the fermentation product at low temperature until the water content is not higher than 13% after the fermentation is finished, thereby obtaining the antiviral compound traditional Chinese medicine preparation for pigs.
Example 3
Antiviral compound traditional Chinese medicine preparation for pigs
1) Raw materials: 3.5 parts of mulberry leaf, 3.5 parts of spanishneedles herb, 1.5 parts of liquorice, 3 parts of gynostemma pentaphylla and 7.5 parts of soybean meal (the total weight of the raw materials is recorded as A);
bacillus subtilis (1:3500 weight ratio to A)
Bacillus licheniformis (1:3000 weight ratio to A)
Lactic acid bacteria (1:4000 weight ratio to A)
Yeast (1:4500 weight ratio to A)
Neutral proteinase (1:6000 weight ratio to A)
Acid proteinase (1:5000 weight ratio to A)
2) The preparation method comprises the following steps: mixing mulberry leaves, spanishneedles herb, liquorice, gynostemma pentaphylla and bean pulp, adding bacillus subtilis, bacillus licheniformis, lactobacillus, saccharomycetes, neutral protease and acid protease, uniformly mixing, adding water to control the water content of the total material to be 35%, fermenting for 3d at 37 ℃, after-ripening for 10d at room temperature, and drying the fermentation product at low temperature until the water content is not higher than 13% after the fermentation is finished, thereby obtaining the antiviral compound traditional Chinese medicine preparation for pigs.
Example 4
Antiviral compound traditional Chinese medicine preparation for pigs
1) Raw materials: 2.5 parts of mulberry leaf, 3.5 parts of spanishneedles herb, 1 part of liquorice, 4 parts of gynostemma pentaphylla and 8 parts of soybean meal (the total weight of the raw materials is recorded as A);
bacillus subtilis (1:3000 weight ratio to A)
Bacillus licheniformis (1:3000 weight ratio to A)
Lactic acid bacteria (1:3500 weight ratio to A)
Yeast (1:3500 weight ratio to A)
Neutral proteinase (1:6000 weight ratio to A)
Acid proteinase (1:6000 weight ratio to A)
2) The preparation method comprises the following steps: mixing mulberry leaves, spanishneedles herb, liquorice, gynostemma pentaphylla and bean pulp, adding bacillus subtilis, bacillus licheniformis, lactobacillus, saccharomycetes, neutral protease and acid protease, uniformly mixing, adding water to control the water content of the total material to be 35%, fermenting for 3d at 37 ℃, after-ripening for 12d at room temperature, and drying the fermentation product at low temperature until the water content is not higher than 13% after the fermentation is finished, thereby obtaining the antiviral compound traditional Chinese medicine preparation for pigs.
Example 5
Antiviral compound traditional Chinese medicine preparation for pigs
1) Raw materials: 5 parts of mulberry leaf, 4 parts of spanishneedles herb, 2 parts of liquorice, 3.5 parts of gynostemma pentaphylla and 7.5 parts of soybean meal (the total weight of the raw materials is recorded as A);
bacillus subtilis (1:3500 weight ratio to A)
Bacillus licheniformis (1:2500 weight ratio to A)
Lactic acid bacteria (1:4000 weight ratio to A)
Yeast (1:5000 weight ratio to A)
Neutral proteinase (1:5000 weight ratio to A)
Acid proteinase (1:5000 weight ratio to A)
2) The preparation method comprises the following steps: mixing mulberry leaves, spanishneedles herb, liquorice, gynostemma pentaphylla and bean pulp, adding bacillus subtilis, bacillus licheniformis, lactobacillus, saccharomycetes, neutral protease and acid protease, uniformly mixing, adding water to control the water content of the total material to be 35%, fermenting for 3d at 37 ℃, after-ripening for 13d at room temperature, and drying the fermentation product at low temperature until the water content is not higher than 13% after the fermentation is finished, thereby obtaining the antiviral compound traditional Chinese medicine preparation for pigs.
Example 6
The compound Chinese medicinal preparation obtained in the example 3 is divided into three dosages of low dosage, medium dosage and high dosage, wherein the dosage is calculated as low dosage 10g/kg, medium dosage 20g/kg and high dosage 40g/kg (calculated as the amount of Chinese medicaments) according to the weight of Chinese medicaments/mice; the pseudorabies virus is challenged and the administration is continued after one week of administration, and the clinical symptoms, the onset time, the onset to death time and the death rate of the mice after the challenge are observed and recorded.
Experimental results show that the death rate of the mice without toxin attack reaches 100% in the third day after toxin attack, obvious pruritus symptoms appear, toxin attack parts drop due to biting hair, drinking water is wasted, and the weight drops sharply. The death rate of the mice in the group of the drug administration is only 25% in the fifth day, the pruritus symptom of the mice in the control group does not appear, the weight rises rapidly after the mice are slightly reduced in the third day, the time for onset is delayed, and the time for onset to death is prolonged.
TABLE 1
Example 7
The compound traditional Chinese medicine preparation obtained in the example 3 is mixed with basic feed according to the proportion of 0.25%, 0.5% and 1% (calculated by the amount of Chinese medicine), and the mixture is fed to weaned piglets growing during 'Du growth' for 28 days. Recording data such as feeding weight gain diarrhea of piglets during an experiment, collecting blood after 28d, separating serum, measuring the total IgG content in the piglet serum, and detecting the virulence condition of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) in a pig group.
The total serum IgG content of the piglets after the compound traditional Chinese medicine preparation is administrated for 28d is shown in figure 2, the diarrhea rate of the piglets in the test period 28d is shown in figure 3, and the feed for the piglets in the test period 28d is shown in figure 4.
Experimental results show that compared with the method without adding medicines, the method has the advantages that the feed-to-weight ratio is obviously reduced, the detection rate of PRRSV in serum is reduced, the diarrhea rate of piglets is obviously reduced, and the total IgG content in serum is obviously improved.
Table 2 weaned piglet toxicity carrying rate
Claims (6)
1. A preparation method of an antiviral compound traditional Chinese medicine preparation for pigs is characterized by comprising the following steps: the traditional Chinese medicine composition is prepared from a traditional Chinese medicine composition, compound bacteria and compound enzyme, wherein the traditional Chinese medicine composition comprises the following components in parts by weight: 2-5 parts of mulberry leaf, 2-5 parts of spanishneedles herb, 1-2 parts of liquorice, 1-5 parts of gynostemma pentaphylla and 5-10 parts of soybean meal;
the composite bacteria comprise bacillus subtilis, bacillus licheniformis, lactobacillus and saccharomycetes, wherein the weight ratio of the bacillus subtilis to the traditional Chinese medicine composition is 1:2000-5000, the weight ratio of the bacillus licheniformis to the traditional Chinese medicine composition is 1:2000-5000, the weight ratio of the lactobacillus to the traditional Chinese medicine composition is 1:2000-5000, and the weight ratio of the saccharomycetes to the traditional Chinese medicine composition is 1:2000-5000;
the complex enzyme consists of neutral protease and acid protease, wherein the weight ratio of the neutral protease to the traditional Chinese medicine composition is 1:5000-10000, and the weight ratio of the acid protease to the traditional Chinese medicine composition is 1:3000-8000;
the preparation method comprises the following steps: mixing folium Mori, herba Bidentis Bipinnatae, glycyrrhrizae radix, herba Gynostemmatis and bean pulp, adding bacillus subtilis, bacillus licheniformis, lactobacillus, yeast, neutral protease and acid protease, mixing, adding water, fermenting, and drying the fermentation product to obtain the antivirus compound Chinese medicinal preparation for pig.
2. The method for preparing the antiviral compound traditional Chinese medicine preparation for pigs, which is characterized in that: the water is added in an amount such that the water content of the total material is 30-40%.
3. The method for preparing the antiviral compound traditional Chinese medicine preparation for pigs, which is characterized in that: the fermentation condition is that the fermentation is 2-4d under the temperature of 35-38 ℃, and the after-ripening is 7-15d under the room temperature.
4. The method for preparing the antiviral compound traditional Chinese medicine preparation for pigs, which is characterized in that: the fermentation product is dried to a moisture content of not more than 13%.
5. The antiviral compound Chinese medicinal preparation for pigs, which is prepared by the preparation method according to any one of claims 1 to 4.
6. The antiviral compound traditional Chinese medicine preparation for pigs according to claim 5, which is characterized in that: the compound traditional Chinese medicine preparation is directly mixed into pig feed according to the weight ratio of 0.5-2 percent of pig feed.
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| CN103960482A (en) * | 2014-05-19 | 2014-08-06 | 湖南博雅特科技有限公司 | Traditional Chinese medicine microbiological feed additive as well as preparation method and application thereof |
| CN105902791A (en) * | 2016-05-17 | 2016-08-31 | 成都奥盛康生物科技有限公司 | Microbiological traditional Chinese medicine preparation for greatly improving animal metabolism and immunity and preparation method thereof |
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| CN103960482A (en) * | 2014-05-19 | 2014-08-06 | 湖南博雅特科技有限公司 | Traditional Chinese medicine microbiological feed additive as well as preparation method and application thereof |
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