CN112972582A - Traditional Chinese medicine composition for preventing and treating animal diarrhea and preparation method thereof - Google Patents
Traditional Chinese medicine composition for preventing and treating animal diarrhea and preparation method thereof Download PDFInfo
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
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- A61K31/4375—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom ortho- or peri-condensed with heterocyclic ring systems the heterocyclic ring system containing a six-membered ring having nitrogen as a ring heteroatom, e.g. quinolizines, naphthyridines, berberine, vincamine
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- A61K35/66—Microorganisms or materials therefrom
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- A61K35/742—Spore-forming bacteria, e.g. Bacillus coagulans, Bacillus subtilis, clostridium or Lactobacillus sporogenes
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- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
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- A61K36/06—Fungi, e.g. yeasts
- A61K36/062—Ascomycota
- A61K36/064—Saccharomycetales, e.g. baker's yeast
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- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
Abstract
The invention belongs to the technical field of veterinary medicines, and discloses a traditional Chinese medicine composition for preventing and treating animal diarrhea and a preparation method thereof, wherein the traditional Chinese medicine composition for preventing and treating animal diarrhea comprises, by mass, 20-25 parts of dandelion extract, 15-20 parts of brucea javanica, 10-15 parts of berberine extract, 10-15 parts of bighead atractylodes rhizome, 10-15 parts of garlic extract, 10-15 parts of isatis root, 10-15 parts of honeysuckle extract, 5-8 parts of Chinese pulsatilla root, 5-8 parts of purslane, 5-10 parts of roxburgh rose root, 4-8 parts of composite probiotic agent, 2-3 parts of notopterygium root and 2-5 parts of hairyvein agrimony. The traditional Chinese medicine composition does not contain chemical reagents such as antibiotics and the like, the animal diarrhea is prevented and treated by pure natural traditional Chinese medicines, the advantages of the traditional Chinese medicines and the composite probiotic agent can be exerted, the injection is not needed, the cost of the raw materials of the traditional Chinese medicines is low, the physique of the fed animals can be adjusted and improved, the growth of intestinal flora microorganisms can be adjusted, and the animal diarrhea can be effectively prevented and treated.
Description
Technical Field
The invention belongs to the technical field of veterinary medicines, and particularly relates to a traditional Chinese medicine composition for preventing and treating animal diarrhea and a preparation method thereof.
Background
At present, animal diarrhea is an acute contact intestinal infectious disease of animals, which is basically characterized by vomiting, diarrhea, appetite reduction and dehydration, and the mortality of sick livestock and poultry is extremely high, thereby causing great economic loss to farmers. In recent years, the epidemic area and the intensity of diarrhea tend to be enlarged and strengthened, which causes high lethality rate to the breeding animals and great economic loss to the breeding industry.
Vaccination is currently the main method of preventing diarrhoea in animals. Although the inactivated vaccine has the advantages of safety, stability and the like, the development of the technology is restricted by the defects of short immune cycle and large immune dose, the inactivated vaccine can only be used for immunization by injection, the protection effect is poor, and the injection can cause the reduction of animal feeding and influence on the yield.
Through the above analysis, the problems and defects of the prior art are as follows: the existing method for preventing and treating animal diarrhea has poor effect and high cost, and can cause the decrease of animal food intake and influence on yield.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a traditional Chinese medicine composition for preventing and treating animal diarrhea and a preparation method thereof.
The invention is realized in such a way that a preparation method of a traditional Chinese medicine composition for preventing and treating animal diarrhea comprises the following steps:
step one, preparing a dandelion extract: removing impurities of the dandelion, and cleaning and drying the dandelion; cutting dried herba Taraxaci into small segments, pulverizing, and sieving with 50-60 mesh sieve to obtain herba Taraxaci powder; adding the obtained dandelion powder into an ethanol solution with the mass fraction of 85%, and carrying out ultrasonic extraction to obtain a dandelion extracting solution; adding acetone into the obtained dandelion extract for extracting for 3-4 times to obtain acetone extract, and removing the acetone by rotary evaporation to obtain dandelion extract; separating and purifying the obtained herba Taraxaci extract by column chromatography, washing with water, eluting with ethyl acetate as eluent, collecting eluent, and drying to obtain herba Taraxaci extract;
step two, preparing the berberine extract: respectively sieving Coptidis rhizoma, cortex Phellodendri and cortex Phellodendri with 20 mesh sieve, soaking in 11-13 times of 0.6% sulfuric acid water solution for 8-10 hr, decocting, filtering, and collecting filtrate; adjusting pH of the obtained filtrate to 11 with lime milk, filtering, adjusting pH of the filtrate to 4-5 with sulfuric acid, adding 5-20% sodium chloride, stirring to dissolve completely, standing for a period of time, and vacuum filtering to obtain crude berberine; adding purified water 20-30 times the weight of the obtained berberine crude product, heating, refluxing, boiling, rapidly filtering, and collecting filtrate; adding 10-15 times of purified water into the residue, boiling for 30min, and filtering; mixing the filtrates, and adjusting pH to 3-4 with sulfuric acid to obtain berberine extract;
step three, preparing the honeysuckle extract: taking honeysuckle, removing impurities, cleaning, drying, grinding the dried honeysuckle into coarse powder, soaking the coarse powder in 65% methanol 8-9 times of the weight of the medicinal materials for 4-5h, performing ultrasonic treatment for 4-5h, filtering, and keeping filter residues for later use; recovering methanol from the filtrate until no alcohol smell exists, and obtaining a first concentrated solution for later use; adding 60-68% ethanol 10-15 times the weight of the medicinal materials into the filter residue, performing ultrasonic treatment for 4-5h, filtering, collecting the filter residue, and recovering ethanol from the filtrate until no ethanol smell exists to obtain a second concentrated solution; adding purified water 10 times the weight of the medicinal materials into the filter residue after the ethanol ultrasonic treatment, heating to 110-120 ℃, reflux extracting for 0.5-1h, and filtering; mixing the obtained filtrate with the first concentrated solution and the second concentrated solution, stirring, concentrating under reduced pressure to obtain soft extract, and vacuum drying to obtain flos Lonicerae extract; meanwhile, garlic is treated, decompressed, converted, neutralized and deodorized to obtain garlic extract for later use;
weighing dandelion extract, brucea javanica, berberine extract, bighead atractylodes rhizome, garlic extract, isatis root, honeysuckle extract, Chinese pulsatilla root, purslane, roxburgh rose root, notopterygium root and hairyvein agrimony according to mass fraction; cleaning weighed rhizoma Atractylodis Macrocephalae, radix Isatidis, radix Pulsatillae, herba Portulacae, radix Rosae Normalis, rhizoma Et radix Notopterygii, and herba Erodii seu Geranii, and oven drying;
step five, crushing the dried corresponding raw materials, sieving the crushed raw materials by a sieve of 80 to 90 meshes, uniformly mixing the crushed raw materials, adding water for soaking, and performing reflux extraction; concentrating the feed liquid to 3 times of the total weight of the traditional Chinese medicinal materials, standing for 12h, precipitating, taking supernatant, uniformly mixing with the dandelion extract, the berberine extract, the garlic extract and the honeysuckle extract, adding the composite probiotic agent, mixing, concentrating under reduced pressure, spray drying, collecting powder, crushing, sieving with a 50-mesh sieve, and uniformly mixing to obtain the traditional Chinese medicine composition for preventing and treating animal diarrhea.
Further, in step one, the ultrasonic extraction includes: ultrasonic extracting at 50-60 deg.C and 80-90kHz for 10-15 min.
Further, in step two, the decocting comprises: heating to 80-90 deg.C, decocting for 2-4 hr.
Further, in the second step, the reflux boiling method comprises: heating to 95-98 deg.C, reflux boiling for 20-25 min.
Further, in step three, the concentration under reduced pressure comprises: setting the vacuum degree at-0.1 to-0.2 MPa and the temperature at 50 to 55 ℃.
Further, in the third step, the garlic is processed, decompressed, converted and neutralized, and deodorized to obtain the garlic extract, which comprises:
peeling Bulbus Allii, cleaning, slicing, soaking in water at room temperature for 3-4 hr, and sieving with 30-40 mesh sieve to obtain Bulbus Allii extractive solution; distilling the obtained Bulbus Allii extractive solution under reduced pressure to obtain non-distillate and distillate; adding alkali into the obtained distillate, adjusting pH to 7-8, standing at room temperature for 25-30min to convert allicin into DADS, and neutralizing with acid; adding a certain amount of active carbon into the neutralized distillate to adsorb Bulbus Allii odor substances, removing Bulbus Allii odor substances adsorbed on active carbon, and recovering to obtain Bulbus Allii extract.
Further, the acid neutralization comprises: hydrochloric acid was added for neutralization.
Further, in step five, the reflux extraction includes:
adding 80-85 deg.C water 5-6 times of the total weight of the raw materials, soaking for 0.5 hr, adding 5-6 times of the total weight of the raw materials, and reflux extracting at 95-98 deg.C for 8 hr.
The traditional Chinese medicine composition for preventing and treating animal diarrhea is prepared by the preparation method of the traditional Chinese medicine composition for preventing and treating animal diarrhea, and the traditional Chinese medicine composition for preventing and treating animal diarrhea is composed of, by mass, 20-25 parts of dandelion extract, 15-20 parts of brucea javanica, 10-15 parts of berberine extract, 10-15 parts of bighead atractylodes rhizome, 10-15 parts of garlic extract, 10-15 parts of radix isatidis, 10-15 parts of honeysuckle extract, 5-8 parts of pulsatilla chinensis, 5-8 parts of purslane, 5-10 parts of rosa roxburghii tratt root, 4-8 parts of composite probiotic agent, 2-3 parts of notopterygium root and 2-5 parts of hairyvein agrimony.
Further, the composite probiotic agent is a composition of any one or more of bacillus subtilis, lactobacillus reuteri or yeast.
By combining all the technical schemes, the invention has the advantages and positive effects that: compared with the method for treating the animal diarrhea by adopting the gentamicin method at the present stage, the traditional Chinese medicine composition for preventing and treating the animal diarrhea, which is provided by the invention, has many advantages, does not contain chemical reagents such as antibiotics, does not need to be injected, is low in cost of raw materials of the traditional Chinese medicine, can adjust and improve the physique of the fed animal, adjusts the growth of microorganisms in intestinal flora, and effectively prevents and treats the animal diarrhea; meanwhile, the traditional Chinese medicine composition does not contain bitter traditional Chinese medicinal materials, has the advantages of good palatability and promotion of the rapid growth of animals, and is beneficial to the healthy growth and weight increment of the animals. The preparation method is simple, low in cost and short in period, and can be used in a large scale. Meanwhile, the traditional Chinese medicine and composite probiotic medicament composition provided by the invention can exert the advantages of the traditional Chinese medicine and the composite probiotic to form complementation, and creates a traditional Chinese medicine and probiotic combined medicament formula, so that the traditional Chinese medicine and composite probiotic medicament composition has the advantages of definite curative effect and natural cost, meets the requirements of safe veterinary medicaments and animal-derived food safety, and is an ideal traditional Chinese medicine composition for treating animal diarrhea.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings needed to be used in the embodiments of the present application will be briefly described below, and it is obvious that the drawings described below are only some embodiments of the present application, and it is obvious for those skilled in the art that other drawings can be obtained from the drawings without creative efforts.
Fig. 1 is a flow chart of a preparation method of a traditional Chinese medicine composition for preventing and treating animal diarrhea provided by the embodiment of the invention.
Fig. 2 is a flow chart of a method for preparing the dandelion extract according to the embodiment of the present invention.
FIG. 3 is a flow chart of a method for preparing berberine extract based on the leaching, pH value adjustment, filtration and extraction of Coptidis rhizoma, Phellodendri cortex and Phellodendri cortex.
Fig. 4 is a flowchart of a method for obtaining a honeysuckle extract by removing impurities from honeysuckle, cleaning, drying, performing ultrasonic reflux, and performing vacuum concentration according to an embodiment of the present invention.
Fig. 5 is a flow chart of a method for processing garlic, reducing pressure, converting, neutralizing and deodorizing to obtain garlic extract according to an embodiment of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Aiming at the problems in the prior art, the invention provides a traditional Chinese medicine composition for preventing and treating animal diarrhea and a preparation method thereof, and the invention is described in detail with reference to the accompanying drawings.
The traditional Chinese medicine composition for preventing and treating animal diarrhea provided by the embodiment of the invention comprises, by mass, 20-25 parts of dandelion extract, 15-20 parts of brucea javanica, 10-15 parts of berberine extract, 10-15 parts of bighead atractylodes rhizome, 10-15 parts of garlic extract, 10-15 parts of radix isatidis, 10-15 parts of honeysuckle extract, 5-8 parts of Chinese pulsatilla root, 5-8 parts of purslane, 5-10 parts of roxburgh rose root, 4-8 parts of composite probiotic agent, 2-3 parts of notopterygium root and 2-5 parts of hairyvein agrimonia herb.
The composite probiotic preparation provided by the embodiment of the invention is a composition of any one or more of bacillus subtilis, lactobacillus reuteri or yeast; the bacillus subtilis is bacillus subtilis ACCC 02136, the lactobacillus reuteri is lactobacillus reuteri ACCC03949, and the yeast is saccharomyces cerevisiae ACCC 21429.
The Bacillus subtilis ACCC 02136 provided by the embodiment of the invention is collected in the agricultural microorganism center of China Committee for culture Collection of microorganisms (ACCC for short, address: No. 12 south China street in Guancun, Haizhou, China academy of agricultural sciences, institute of agricultural resources and agricultural regionalism, postal code 100081) at 20.7.2007, and the strain can be obtained from the agricultural microorganism center of China Committee for culture Collection by the public from the collection date. Bacillus subtilis ACCC 02136 is hereinafter referred to simply as Bacillus subtilis ACCC 02136.
The Lactobacillus reuteri (Lactobacillus reuteri) ACCC03949 provided by the embodiment of the invention is collected in the agricultural microorganism center of China Committee for culture Collection of microorganisms (ACCC for short, address: No. 12 south street of Guancun in Haita district, Beijing, China academy of agricultural sciences, institute of agricultural resources and agricultural divisions, postal code 100081) at 6.15.2008, and the strain can be obtained by the public from the collection date through the agricultural microorganism center of the Committee for culture Collection of microorganisms in China. Lactobacillus reuteri ACCC03949 is hereinafter abbreviated Lactobacillus reuteri ACCC 03949.
The Saccharomyces cerevisiae (ACCC 21429) provided by the embodiment of the invention is collected in the agricultural microorganism center of China Committee for culture Collection of microorganisms (ACCC for short, address: No. 12 south street of Guancun in Haidian lake area of Beijing, institute of agricultural resources and agricultural regionalism, postal code 100081) in 24.2.2017, and the strain can be obtained by the public from the agricultural microorganism center of China Committee for culture Collection of microorganisms from the collection date. Saccharomyces cerevisiae ACCC21429 is hereinafter referred to as Saccharomyces cerevisiae ACCC 21429.
As shown in fig. 1, the preparation method of the traditional Chinese medicine composition for preventing and treating diarrhea of animals provided by the embodiment of the invention comprises the following steps:
s101, preparing a dandelion extract: cleaning dandelion, crushing, ultrasonically extracting, secondarily extracting and purifying to obtain a dandelion extract; meanwhile, berberine extract is prepared by leaching coptis chinensis, phellodendron amurense and phellodendron amurense, adjusting pH value, filtering and extracting;
s102, removing impurities from honeysuckle, cleaning, drying, performing ultrasonic reflux, and concentrating under reduced pressure to obtain a honeysuckle extract; processing Bulbus Allii, reducing pressure, converting, neutralizing, and deodorizing to obtain Bulbus Allii extract;
s103, weighing dandelion extract, berberine extract, bighead atractylodes rhizome, garlic extract, isatis root, honeysuckle extract, Chinese pulsatilla root, purslane, roxburgh rose root, notopterygium root and hairyvein agrimony according to mass fraction; cleaning weighed rhizoma Atractylodis Macrocephalae, radix Isatidis, radix Pulsatillae, herba Portulacae, radix Rosae Normalis, rhizoma Et radix Notopterygii, and herba Erodii seu Geranii, and oven drying;
s104, crushing the dried corresponding raw materials, sieving the crushed raw materials by a sieve of 80-90 meshes, uniformly mixing the crushed raw materials, adding water with the weight 5-6 times of the total weight of the raw materials and the temperature of 80-85 ℃ to soak the raw materials for 0.5h, and then adding water with the weight 5-6 times of the total weight of the raw materials to perform reflux extraction for 8h at the temperature of 95-98 ℃;
s105, concentrating the feed liquid, standing for 12 hours when the feed liquid is concentrated to be 3 times of the total weight of the traditional Chinese medicinal materials, precipitating, taking supernate, uniformly mixing the supernate with the dandelion extract, the berberine extract, the garlic extract and the honeysuckle extract, concentrating under reduced pressure, carrying out spray drying, collecting powder, crushing, sieving with a 50-mesh sieve, and uniformly mixing to obtain the traditional Chinese medicine composition for preventing and treating the animal diarrhea.
As shown in fig. 2, in step S101, the preparation of the dandelion extract according to the embodiment of the present invention includes:
s201, removing impurities of the dandelion, and cleaning and drying the dandelion; cutting dried herba Taraxaci into small segments, pulverizing, and sieving with 50-60 mesh sieve to obtain herba Taraxaci powder;
s202, adding the obtained dandelion powder into an ethanol solution with the mass fraction of 85%, and carrying out ultrasonic extraction to obtain a dandelion extracting solution;
s203, adding acetone into the obtained dandelion extracting solution for extraction for 3-4 times to obtain acetone extracting solution, and performing rotary evaporation to remove the acetone to obtain dandelion extract;
s204, separating and purifying the obtained dandelion extract by using a column chromatography method, washing with water, continuously eluting by using ethyl acetate as an eluent, collecting the eluent, and drying to obtain the dandelion extract.
In step S202, the ultrasound extraction includes: ultrasonic extracting at 50-60 deg.C and 80-90kHz for 10-15 min.
As shown in fig. 3, in step S101, the berberine extract prepared based on the leaching, pH adjustment, filtration and extraction of coptis chinensis, phellodendron amurense and phellodendron amurense provided by the embodiment of the present invention comprises:
s301, respectively sieving coptis chinensis, phellodendron amurense and phellodendron amurense through a 20-mesh sieve, soaking 11-13 times of 0.6% sulfuric acid aqueous solution by weight for 8-10 hours, decocting, filtering, and collecting filtrate;
s302, adjusting the pH value of the obtained filtrate to 11 by using lime milk, filtering, adjusting the pH value of the filtrate to 4-5 by using sulfuric acid, adding 5-20% of sodium chloride, stirring until the sodium chloride is completely dissolved, standing for a period of time, and performing suction filtration to obtain a crude berberine product;
s303, adding purified water of which the weight is 20-30 times that of the obtained berberine crude product, heating to 95-98 ℃, refluxing and boiling for 20-25min, quickly performing suction filtration, and collecting filtrate; adding 10-15 times of purified water into the residue, boiling for 30min, and filtering;
s304, combining the two filtrates, and adding sulfuric acid into the filtrate to adjust the pH value to 3-4 to obtain the berberine extract.
In step S301, the decoction provided in the embodiment of the present invention includes: heating to 80-90 deg.C, decocting for 2-4 hr.
As shown in fig. 4, in step S102, the honeysuckle extract obtained by removing impurities from honeysuckle, cleaning, drying, performing ultrasonic reflux, and concentrating under reduced pressure according to an embodiment of the present invention includes:
s401, removing impurities from honeysuckle, cleaning and drying, grinding the dried honeysuckle into coarse powder, soaking the coarse powder in 65% methanol 8-9 times of the weight of the medicinal materials for 4-5 hours, performing ultrasonic treatment for 4-5 hours, and filtering to obtain filter residues for later use; recovering methanol from the filtrate until no alcohol smell exists, and obtaining a first concentrated solution for later use;
s402, adding 60-68% of ethanol which is 10-15 times of the weight of the medicinal materials into the filter residue, carrying out ultrasonic treatment for 4-5h, filtering, keeping the filter residue for later use, and recovering ethanol from the filtrate until no ethanol smell exists, so as to obtain a second concentrated solution for later use;
s403, adding purified water which is 10 times of the weight of the medicinal materials into the filter residue after the ethanol ultrasonic treatment, heating to 110-120 ℃, reflux extracting for 0.5-1h, and filtering;
s404, mixing the obtained filtrate with the first concentrated solution and the second concentrated solution, uniformly stirring, concentrating under reduced pressure to obtain a thick paste extract, and performing vacuum drying to obtain the honeysuckle extract.
The decompression concentration provided by the embodiment of the invention comprises the following steps: setting the vacuum degree at-0.1 to-0.2 MPa and the temperature at 50 to 55 ℃.
As shown in fig. 5, in step S102, after the garlic is processed, decompressed, transformed and neutralized, and deodorized, a garlic extract is obtained, which includes:
s501, peeling garlic, cleaning, slicing, adding water, soaking at room temperature for 3-4h, and sieving with a 30-40-mesh sieve to obtain a garlic extract;
s502, carrying out reduced pressure distillation on the obtained garlic extract to obtain non-distillate and distillate; adding alkali into the obtained distillate, adjusting pH to 7-8, standing at room temperature for 25-30min to convert allicin into DADS, and neutralizing with acid;
s503, adding a certain amount of activated carbon into the neutralized distillate to adsorb the garlic odor substances, performing desorption treatment on the garlic odor substances adsorbed on the activated carbon, and recovering to obtain the garlic extract.
The acid neutralization provided by the embodiment of the invention comprises the following steps: hydrochloric acid was added for neutralization.
The technical solution of the present invention is further described below in connection with pharmacological analysis.
Taraxacum mongolicum hand-held, Mazz, Taraxacum mongolicum Kitam, a Compositae plant, or a dried whole plant of a tree species of the same genus. Collected in spring to autumn when the flower begins to bloom, removed of impurities, cleaned and dried, bitter, sweet and cold in nature, enters liver and stomach channels, has the effects of clearing heat and removing toxicity, relieving swelling and dissipating stagnation, inducing diuresis and treating stranguria when being used for medicine, and can be clinically used for treating furuncle pyogenic infections, acute mastitis, scrofula, conjunctival congestion, pharyngalgia, pulmonary abscess, damp-heat jaundice and stranguria with astringency and pain.
Brucea javanica (L.) Merr, also known as brucea javanica and ya Dan Zi, is fruit of brucea javanica (L.) Merr. Harvesting in autumn when the fruit is mature, removing impurities of branches and leaves, cleaning, and drying in the sun. When used, the shell is removed and the whole kernel is unprocessed. Nature and taste: bitter and cold with slight toxicity. The functional indications are as follows: clear heat, dry dampness, kill parasites and remove toxicity. It is indicated for dysentery, chronic diarrhea, malaria, hemorrhoids, furunculosis, excrescence wart and clavus.
Berberine, an alkaloid with a typical bitter taste, is often present in the form of a hydrochloride salt, called berberine hydrochloride. Berberine has remarkable antibacterial activity, is present in various traditional Chinese medicines such as coptis chinensis, phellodendron amurense, barberry root, mahonia stem and fibraurea stem, and is the main effective component of the medicinal materials.
Bai Zhu belongs to qi tonics. Nature, flavor and meridian tropism: bitter, sweet and warm. It enters spleen and stomach meridians. Has the functions of invigorating spleen and stomach, eliminating dampness and promoting diuresis, preventing miscarriage and stopping sweating. Indications, diarrhea due to spleen deficiency, abdominal distension, edema, sweating due to debility, threatened abortion.
Garlic, the underground bulb of garlic (Allium Sativum L.) of Allium of Liliaceae, is pungent and warm in taste, and has unique effects of activating qi-flowing, warming spleen and stomach, removing toxic substances, killing parasites, etc. In veterinary medicine, the traditional Chinese medicine composition is mainly used for treating chronic grass eating failure, abdominal distension and pain, lower diseases, abdominal pain due to parasitic infestation, fish enteritis and the like.
Radix Isatidis is divided into radix Isatidis and radix Isatidis, and the radix Isatidis is derived from root of Isatis tinctoria and radix Isatidis of Brassicaceae; radix Isatidis is rhizome and root of herba Kalimeridis of Acanthaceae; has effects of clearing away heat and toxic materials, cooling blood, relieving swelling, and relieving sore throat. The water extract has effect in inhibiting Bacillus subtilis, Staphylococcus aureus, Spanish Auricularia, Escherichia coli, Salmonella typhi, Bacillus paratyphi, Shigella dysenteriae, and enteritis bacillus.
Honeysuckle flower, flower bud of Lonicera japonica Thunb. Nature and taste: sweet and cold in nature. It enters lung and stomach meridians. The functions are mainly as follows: clear heat and remove toxicity. It is indicated for epidemic febrile disease, fever, bloody dysentery, carbuncle and external diseases, swelling, scrofula and anal fistula.
Pulsatilla chinensis belongs to heat-clearing and toxicity-removing herbs. The property and flavor of meridian tropism are bitter and cold. It enters stomach and large intestine meridians. Has the functions of clearing away heat and toxic material, cooling blood and stopping dysentery. The main indications are heat-toxin bloody dysentery and damp-heat yellow intestine.
Purslane, also called purslane, longevous vegetable, five-element grass and the like, is an annual fleshy herbaceous plant of purslane family, is widely distributed in temperate and tropical regions all over the world, is a plant which is recognized as being homologous to medicine and food in the medical and food industries, has the functions of clearing heat and detoxicating, cooling blood and stopping bleeding, and is used for treating heat toxin bloody dysentery, carbuncle swelling and furuncle, eczema, erysipelas, snake and insect bite, hematochezia, hemorrhoidal bleeding and metrorrhagia and metrostaxis.
Roxburgh rose root, Iso-name Chenopodium root, and Chenopodium root. Is root of Rosa roxburghii Tratt.f. Normalis Rehd.et Wils. Collected between 7-8 months. The root and stem both contain tannin. The root bark of Sichuan province contains tannin. But also contains 2.4 percent of vitamin B and 2.8 percent of vitamin P. Nature and taste: gansu acid, Ping. The functions are mainly as follows: strengthening the stomach, promoting digestion, checking diarrhea, and controlling nocturnal emission. It is indicated for stomach ache, diarrhea, dysentery, seminal emission, metrorrhagia, leukorrhagia, chronic cough.
Notopterygii rhizoma, Umbelliferae, Notopterygii rhizoma plant, with warm nature and exterior cold dispelling effects; dispelling wind-damp; benefiting the joints. Wind-cold affecting the exterior; headache without sweating; edema due to wind and water; sores and ulcers with pyogenic infections. Can be used for treating sexual impotence, spermatorrhea, frequent micturition, lumbago, gonalgia, asthma due to kidney deficiency, and diarrhea before dawn; it is used externally to treat vitiligo and alopecia areata. Relieve exterior syndrome, dispel cold, expel wind and remove dampness, and alleviate pain.
The extract of herba Erodii seu Geranii contains flavonoid, catechin, and tannin, and can inhibit replication of multiple viruses, wherein the extract has most obvious effect in resisting influenza virus, can reduce infectivity of various influenza virus strains, and also can inhibit growth of in vitro Staphylococcus aureus and Candida albicans, and has antibacterial and sterilizing effects.
The technical solution of the present invention is further described with reference to the following specific examples.
In a sick pig farm in a certain area, due to the influence of cold, newly purchased piglets and weaned piglets are diarrhea (about 500 piglets in the whole group), piglets are thin, have swelling condition, are obviously dehydrated, have a long course of disease, and die after being dehydrated finally. 80 piglets suffering from severe diarrhea are randomly selected and divided into two groups, namely 40 piglets in the first group and 40 piglets in the second group, and all the piglets are subjected to feeding management under the same nutrition level and management conditions.
The traditional Chinese medicine composition for preventing and treating animal diarrhea prepared by the invention is added to the first group of sick piglet diarrhea, and is mixed with 250 kg of materials for every 1kg of traditional Chinese medicine composition for continuous use for 15 days;
oxytetracycline is added into the second group of sick piglet diarrhea, and the mixture is mixed with 250 kg of oxytetracycline per 1kg of the mixture and is continuously used for 15 days.
Statistical treatment of test data: the test is managed by a specially-assigned person, and the spirit, body temperature and excrement conditions of the piglets are observed and recorded at any time; and (4) when the experiment is finished, counting the cure rate and the death rate of the piglets according to a conventional statistical method of zootechnics. After 10-day experiments and statistical arrangement of experimental data, the first group cures 35, dies 2 and heals 3 better but not heals; the second group had 34 healings, 5 deaths, and 11 healed but not healed. Therefore, the test obtains better results, and the traditional Chinese medicine composition for preventing and treating animal diarrhea prepared by the invention can effectively relieve and treat animal diarrhea without side effect, and is an ideal traditional Chinese medicine composition for treating animal diarrhea.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention, and the scope of the present invention is not limited thereto, and any modification, equivalent replacement, and improvement made by those skilled in the art within the technical scope of the present invention disclosed herein, which is within the spirit and principle of the present invention, should be covered by the present invention.
Claims (10)
1. A preparation method of a traditional Chinese medicine composition for preventing and treating animal diarrhea is characterized by comprising the following steps:
step one, preparing a dandelion extract: removing impurities of the dandelion, and cleaning and drying the dandelion; cutting dried herba Taraxaci into small segments, pulverizing, and sieving with 50-60 mesh sieve to obtain herba Taraxaci powder; adding the obtained dandelion powder into an ethanol solution with the mass fraction of 85%, and carrying out ultrasonic extraction to obtain a dandelion extracting solution; adding acetone into the obtained dandelion extract for extracting for 3-4 times to obtain acetone extract, and removing the acetone by rotary evaporation to obtain dandelion extract; separating and purifying the obtained herba Taraxaci extract by column chromatography, washing with water, eluting with ethyl acetate as eluent, collecting eluent, and drying to obtain herba Taraxaci extract;
step two, preparing the berberine extract: respectively sieving Coptidis rhizoma, cortex Phellodendri and cortex Phellodendri with 20 mesh sieve, soaking in 11-13 times of 0.6% sulfuric acid water solution for 8-10 hr, decocting, filtering, and collecting filtrate; adjusting pH of the obtained filtrate to 11 with lime milk, filtering, adjusting pH of the filtrate to 4-5 with sulfuric acid, adding 5-20% sodium chloride, stirring to dissolve completely, standing for a period of time, and vacuum filtering to obtain crude berberine; adding purified water 20-30 times the weight of the obtained berberine crude product, heating, refluxing, boiling, rapidly filtering, and collecting filtrate; adding 10-15 times of purified water into the residue, boiling for 30min, and filtering; mixing the filtrates, and adjusting pH to 3-4 with sulfuric acid to obtain berberine extract;
step three, preparing the honeysuckle extract: taking honeysuckle, removing impurities, cleaning, drying, grinding the dried honeysuckle into coarse powder, soaking the coarse powder in 65% methanol 8-9 times of the weight of the medicinal materials for 4-5h, performing ultrasonic treatment for 4-5h, filtering, and keeping filter residues for later use; recovering methanol from the filtrate until no alcohol smell exists, and obtaining a first concentrated solution for later use; adding 60-68% ethanol 10-15 times the weight of the medicinal materials into the filter residue, performing ultrasonic treatment for 4-5h, filtering, collecting the filter residue, and recovering ethanol from the filtrate until no ethanol smell exists to obtain a second concentrated solution; adding purified water 10 times the weight of the medicinal materials into the filter residue after the ethanol ultrasonic treatment, heating to 110-120 ℃, reflux extracting for 0.5-1h, and filtering; mixing the obtained filtrate with the first concentrated solution and the second concentrated solution, stirring, concentrating under reduced pressure to obtain soft extract, and vacuum drying to obtain flos Lonicerae extract; meanwhile, garlic is treated, decompressed, converted, neutralized and deodorized to obtain garlic extract for later use;
weighing dandelion extract, brucea javanica, berberine extract, bighead atractylodes rhizome, garlic extract, isatis root, honeysuckle extract, Chinese pulsatilla root, purslane, roxburgh rose root, notopterygium root and hairyvein agrimony according to mass fraction; cleaning weighed rhizoma Atractylodis Macrocephalae, radix Isatidis, radix Pulsatillae, herba Portulacae, radix Rosae Normalis, rhizoma Et radix Notopterygii, and herba Erodii seu Geranii, and oven drying;
step five, crushing the dried corresponding raw materials, sieving the crushed raw materials by a sieve of 80 to 90 meshes, uniformly mixing the crushed raw materials, adding water for soaking, and performing reflux extraction; concentrating the feed liquid to 3 times of the total weight of the traditional Chinese medicinal materials, standing for 12h, precipitating, taking supernatant, uniformly mixing with the dandelion extract, the berberine extract, the garlic extract and the honeysuckle extract, adding the composite probiotic agent, mixing, concentrating under reduced pressure, spray drying, collecting powder, crushing, sieving with a 50-mesh sieve, and uniformly mixing to obtain the traditional Chinese medicine composition for preventing and treating animal diarrhea.
2. The method for preparing a composition for preventing and treating diarrhea in an animal according to claim 1, wherein in the first step, the ultrasonic extraction comprises: ultrasonic extracting at 50-60 deg.C and 80-90kHz for 10-15 min.
3. The method of claim 1, wherein the step two, the decocting comprises: heating to 80-90 deg.C, decocting for 2-4 hr.
4. The method for preparing a composition for preventing and treating diarrhea in animals according to claim 1, wherein in the second step, the reflux boiling method comprises: heating to 95-98 deg.C, reflux boiling for 20-25 min.
5. The method for preparing a composition for preventing and treating diarrhea in animals according to claim 1, wherein the concentration under reduced pressure in step three comprises: setting the vacuum degree at-0.1 to-0.2 MPa and the temperature at 50 to 55 ℃.
6. The method of claim 1, wherein the step three, the garlic is processed, decompressed, transformed and neutralized, and deodorized to obtain the garlic extract, which comprises:
peeling Bulbus Allii, cleaning, slicing, soaking in water at room temperature for 3-4 hr, and sieving with 30-40 mesh sieve to obtain Bulbus Allii extractive solution; distilling the obtained Bulbus Allii extractive solution under reduced pressure to obtain non-distillate and distillate; adding alkali into the obtained distillate, adjusting pH to 7-8, standing at room temperature for 25-30min to convert allicin into DADS, and neutralizing with acid; adding a certain amount of active carbon into the neutralized distillate to adsorb Bulbus Allii odor substances, removing Bulbus Allii odor substances adsorbed on active carbon, and recovering to obtain Bulbus Allii extract.
7. The method of claim 6, wherein the acid neutralization comprises: converting allicin into DADS, and neutralizing with hydrochloric acid; adding a certain amount of active carbon into the neutralized distillate to adsorb Bulbus Allii odor substances, removing Bulbus Allii odor substances adsorbed on active carbon, and recovering to obtain Bulbus Allii extract.
8. The method for preventing and treating diarrhea in animals according to claim 1, wherein in the fifth step, the reflux extraction comprises:
adding 80-85 deg.C water 5-6 times of the total weight of the raw materials, soaking for 0.5 hr, adding 5-6 times of the total weight of the raw materials, and reflux extracting at 95-98 deg.C for 8 hr.
9. The traditional Chinese medicine composition for preventing and treating animal diarrhea, which is prepared by the preparation method of the traditional Chinese medicine composition for preventing and treating animal diarrhea according to any one of claims 1 to 8, is characterized by comprising, by mass, 20-25 parts of dandelion extract, 15-20 parts of brucea javanica, 10-15 parts of berberine extract, 10-15 parts of bighead atractylodes rhizome, 10-15 parts of garlic extract, 10-15 parts of isatis root, 10-15 parts of honeysuckle extract, 5-8 parts of pulsatilla chinensis, 5-8 parts of purslane, 5-10 parts of rosa roxburghii tratt root, 4-8 parts of composite probiotic agent, 2-3 parts of notopterygium root and 2-5 parts of hairyvein agrimony.
10. The traditional Chinese medicine composition for preventing and treating diarrhea of animals according to claim 9, wherein the complex probiotic is a composition of any one or more of bacillus subtilis, lactobacillus reuteri or yeast.
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| CN102318771A (en) * | 2011-09-20 | 2012-01-18 | 广东粤海饲料集团有限公司 | Chinese medicinal herb disease-resistant additive for prawn feed |
| CN110478397A (en) * | 2019-09-18 | 2019-11-22 | 四川成康动物药业有限公司 | Pharmaceutical composition and preparation method thereof for preventing and treating animal diarrhea |
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| CN102318771A (en) * | 2011-09-20 | 2012-01-18 | 广东粤海饲料集团有限公司 | Chinese medicinal herb disease-resistant additive for prawn feed |
| CN110478397A (en) * | 2019-09-18 | 2019-11-22 | 四川成康动物药业有限公司 | Pharmaceutical composition and preparation method thereof for preventing and treating animal diarrhea |
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| CN115554364A (en) * | 2022-10-24 | 2023-01-03 | 甘孜藏族自治州畜牧业科学研究所 | Traditional Chinese medicine and Tibetan medicine composition for preventing and treating diarrhea of yaks |
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