CN103652442A - Feed additive for improving growth immune function of broilers, preparation method and application - Google Patents
Feed additive for improving growth immune function of broilers, preparation method and application Download PDFInfo
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Abstract
The invention discloses a feed additive for improving growth immune function of broilers, and the feed additive includes astragalus, folium isatidis, herba leonuri, hawthorn, malt, medicated leaven, pine needle powder, gastrodia armillaria mellea powder and a carrier. The invention also provides a preparation method and an application method of the feed additive. A variety of Chinese herbal medicines and the gastrodia armillaria mellea are used as main raw materials for preparing the feed additive to realize the coordination linkage of the Chinese herbal medicines and the gastrodia armillaria mellea, the broiler daily feed intake and daily weight gain are significantly increased, the mortality and the feed conversion ratio are decreased significantly and the broiler immunity (including immune organ indexes, hemagglutination inhibition antibody titer, peripheral lymphocyte transformation rate, NK (natural killer) cell activity, IL (interleukin) activity indexes) is significantly enhanced.
Description
Technical field
The invention belongs to field of feed additive technology, specifically relate to a kind of feed addictive, preparation method and application that improves growth of meat chicken immune performance.
Background technology
Along with the development of herding intensive culture production model, the factor such as feeding environment worsens, nutrient is unbalanced or not enough, has caused animal immunizing power low, show as poor growth, feedstuff-meat ratio rises, and the death rate raises, raiser's loss, and then affect livestock products market supply.Meanwhile, intensive lower stress effect, makes animal show as restlessness, this be stress external manifestation, for anti-stress, animals consuming a large number of nutrients is for the various metabolism of balance, its feeding achievement reduces.In production, need suitable product and technology to solve above two hang-ups.
Undeniable, in the past few decades, antibiotic is being prevented and treated Animal diseases as feed addictive, improves breeding performonce fo animals aspect and has played positive role.But the long-term use of antibiotics additive, causes many adverse consequences, makes germ produce drug resistance, and then the residual health that has influence on people in livestock products.For this reason, developed country is used and has carried out strict restriction antibiotic, and within 2006, European Union forbids fodder antibiotics comprehensively.The new feed addictive management rules of China have also been made strict regulations to the use of Antibiotic Additive.
Therefore, Antistress agent is exempted from the increasing of the safety of development of new, is to overcome in Production of Livestock and Poultry process, to occur hypoimmunity, improve Safety of Livestock Products ' Quality and overcome the optimal path that stress wait problem.Animal nutrition scholar, finding natural active matter, substitutes the production performance that antibiotic improves animal at present.Mainly researched and developed the multiple additives such as probiotic, oligosaccharides, plant extracts, Chinese herbal medicine both at home and abroad, to improving premunition, promoting production performance performance to have certain effect, but effect does not have breakthrough progress.Publication number be CN1788779A Patent Application Publication a kind of poultry body-strengthening conditioning agent for fowl, this conditioning agent selects prepared from traditional Chinese medicines to form, and includes folium isatidis, golden cypress, the root of large-flowered skullcap, garlic, Radix Isatidis, raw hawthorn, the Radix Astragali, cactus, the bark of ash, radix pseudostellariae, the capsule of weeping forsythia, ginseng, the leaf of bamboo, raw Radix Glycyrrhizae, Fructus Hordei Germinatus, dried orange peel, honeysuckle, dark plum, motherwort.In the document, comprise 19 kinds of traditional Chinese medicinal components, Chinese medicine cost is higher, although improved to a certain extent kind adult livability and laying rate, but not obvious for the raising of immunity (comprising Immune Organs Index, HI antibody titer, periphery lymphocyte conversion ratio, NK cytoactive, IL activity index) that improves the broiler chicken later stage, and feedstuff-meat ratio is higher, cannot meet the demand of existing feeding of broiler producer.
We are devoted in the research of polysaccharide when having accepted multinomial provinces and cities scientific research project always, especially on fungi polysaccharide and bacterial polysaccharides, because they can carry out suitability for industrialized production by fermentation, cost is controlled, and environmental protection pressure is little, does not have the problem of resource shortage.Primary Study is found, adds armillaria mellea accreting with Rhizoma Gastrodiae powder (main component is armillaria mellea polysaccharide, has other several functions product concurrently) and can make piglet skin ruddy glossy, shows as peace and quiet, and feed conversion rate improves, and the incidence of disease and expenses for medicine decline.In conjunction with our research as feed addictive at Chinese herbal medicine in early days, comprise and improve immunity calm Chinese medicine preparation and process technology (cellular level pulverizing technology), can be used as supporting technology and further investigate, form brand-new increasing and exempt from Antistress agent, be expected to solve the above-mentioned difficult problem in producing.Through Ministry of Education's current awareness search work station (Z09), look into new confirmation, armillaria mellea accreting with Rhizoma Gastrodiae powder is had no to report as the research of feed addictive both at home and abroad, armillaria mellea accreting with Rhizoma Gastrodiae powder and Chinese herbal medicine combination also have no report as the research of feed addictive.
Summary of the invention
The invention provides a kind of feed addictive that improves growth of meat chicken immune performance, it is that primary raw material prepares that this additive adopts multiple Chinese herbal medicine and armillaria mellea accreting with Rhizoma Gastrodiae, can significantly improve broiler production and immune performance.
The present invention also provides a kind of preparation method of feed addictive of above-mentioned raising growth of meat chicken immune performance, and transformation of the way Preparation Method technique is simple, can be suitable for suitability for industrialized production.
The present invention also provides a kind of application process of feed addictive of above-mentioned raising growth of meat chicken immune performance, in application process, can make broiler chicken daily ingestion amount, daily gain significantly improve, and the death rate and feedstuff-meat ratio significantly decline; Immunity of meat chickens significantly strengthens.
A feed addictive that improves growth of meat chicken immune performance, comprising: the Radix Astragali, folium isatidis, motherwort, hawthorn, Fructus Hordei Germinatus, Divine Comedy, pine needle meal, armillaria mellea accreting with Rhizoma Gastrodiae powder and carrier.
As preferably, according to percentage by weight, calculate, the feed addictive of described raising growth of meat chicken immune performance comprises:
As further preferably, according to percentage by weight, calculate, the feed addictive of described raising growth of meat chicken immune performance comprises:
Described carrier is generally zeolite powder, also can select as required drug carrier in other.
In above-mentioned each traditional Chinese medicinal components:
The Radix Astragali: slightly warm in nature, taste is sweet, returns lung, spleen, liver, kidney channel, there is enhanced machine body immunity function, protect the liver, diuresis, anti-ageing, anti-stress, step-down and antibacterial effect widely, be used for the treatment of night sweat due to yin deficiency, syndrome of deficiency of lung qi, hidrosis edema.
Folium isatidis: cold in nature, bitter, returns liver, the heart, stomach warp, has heat-clearing, removing toxic substances, cool blood, the effect of hemostasis, is used for the treatment of heat poison and sends out the diseases such as spot, erysipelas, abscess of throat, aphthae, sore and toxic.
Motherwort: cool in nature, bitter, the thoughts of returning home, liver, urinary bladder channel.The effect with invigorate blood circulation, dispel silt, menstruation regulating, the water that disappears, is used for the treatment of irregular menstruation, threatened abortion difficult labour, and placenta retension, postpartum anemic fainting, abdominal pain due to blood stasis, metrorrhagia and metrostaxis, hematuria, rushes down blood, the carbuncle sore that swells.
Hawthorn: slightly warm in nature, taste acid, sweet, returns spleen, stomach, Liver Channel, has promoting digestion and removing indigestion, the effect of promoting blood circulation to remove blood stasis cures mainly carnivorous stagnantly, stomachache is had loose bowels, postpartum stasis blocking, hernia pain.
Fructus Hordei Germinatus: property is flat, and taste is sweet, returns spleen, stomach through, Liver Channel, has promote qi circulation digestion promoting, and spleen benefiting and stimulating the appetite moves back the effect of newborn dissipate-swelling, and cure mainly dyspepsia and do not disappear, abdominal distention, insufficiency of the spleen food is few, and newborn sweat smoulders, swollen breasts, women's ablactation.
Divine Comedy: warm in nature, bitter, returns spleen, stomach, large intestine channel, has reinforcing spleen to promote digestion, regulating QI to expel dampness, the effect of inducing sweat, cures mainly the feeling of stuffiness in chest of getting ill from overeating, and stomachache is vomited and diarrhoea, dysentery, cold headache, children's hinders hungry mistake and satisfies.
Pine needle meal: the leaf by masson pine, Pinus taiwanesis, black pine, Japanese red pine, larch, Chinese pine, pinus sylvestris var. mongolica, dragon spruce and fir is processed into, contains abundant thick protein, fat, trace element and vitamin etc.
Above-mentioned Chinese medicine all can be selected from commercially available prod.
The present invention also provides the method for the feed addictive of preparing above-mentioned raising growth of meat chicken immune performance, comprising:
(1) get required Chinese medicine, section, mix, mixed mixture is carried out to coarse crushing, then carry out cellular level pulverizing, it is 25～40 microns (approximately fineness is 350～500 orders) that mixture is crushed to average grain diameter, then adds first's carrier, mix, obtain sub-micron Chinese herbal medicine;
(2) the sub-micron Chinese herbal medicine obtaining and armillaria mellea accreting with Rhizoma Gastrodiae powder are mixed, then add second portion carrier, mix and obtain feed addictive.
In step (1), coarse crushing can adopt existing equipment, and general corase grind is to 60 order left and right.Carry out cellular level pulverizing and generally adopt QYF type fluidized bed air flow crusher.The carrier adopting is generally the zeolite powder that fineness is 100 order left and right.
Armillaria mellea accreting with Rhizoma Gastrodiae powder derives from Zhejiang pharmaceutical Co. Ltd of Wan Feng group fermenting and producing, and this product is sold on market.
The invention provides a kind of application process of feed addictive of above-mentioned raising growth of meat chicken immune performance, this feed addictive is when reality is used, and addition is the 0.1-0.5% of feed weight.Further preferred addition is the 0.1-0.3% of feed weight, more more preferably 0.2%.
Compared with prior art, beneficial effect of the present invention is embodied in:
It is that primary raw material prepares feed addictive by multiple Chinese herbal medicine and armillaria mellea accreting with Rhizoma Gastrodiae that the present invention adopts, realize the coordination linkage of Chinese herbal medicine and armillaria mellea accreting with Rhizoma Gastrodiae, can make broiler chicken daily ingestion amount and daily gain significantly improve, feedstuff-meat ratio significantly declines, immunity significantly strengthens, and without any harmful effect.
The specific embodiment
Embodiment 1
Respectively by the Radix Astragali, folium isatidis (leaf of Radix Isatidis), motherwort, hawthorn, Fructus Hordei Germinatus, Divine Comedy, pine needle meal is dried, then weigh respectively 200 grams of the Radixs Astragali, 200 grams of folium isatidis (leaf of Radix Isatidis), 100 grams of motherworts, 50 grams of hawthorn, 50 grams, Fructus Hordei Germinatus, 50 grams of Divine Comedy, 150 grams of pine needle meals, then section arranges, mix, first mixed mixture is carried out to 60 order coarse crushings, then adopt QYF type fluidized bed air flow crusher to carry out cellular level pulverizing, it is 25～40 microns (approximately fineness is 350～500 orders) that mixture is crushed to average grain diameter, add again 200 grams of carrier zeolite powders (fineness is 100 orders), total amount reaches 1000 grams, again mix, obtain this sub-micron Chinese herbal medicine.
Above-mentioned 500 grams of sub-micron Chinese herbal medicines are mixed with 250 grams of armillaria mellea accreting with Rhizoma Gastrodiae powder, then add 250 grams of carrier zeolite powders (fineness is 100 orders), total amount reaches 1000 grams, again mixes, and obtains this feed addictive.
The experiment that the formula of Application Example 1 is carried out
Test site: Jiaxing joyful cultivation Co., Ltd.
Time: 2011.3.7-2011.4.19,42 days by a definite date.
Experimental animal: test selects 1 age in days to like to pull out young young 320 (male and female half and half) of commercial generation meat increasingly, initial body weight difference not significantly (P>0.05) between group in group.Be divided at random 4 processed group, establish 4 repetitions for every group, each repeats 20 chickens.
Test daily ration: adopt corn-soybean meal basal diet, with reference to U.S. NRC(1998) meat chick nutritional need is formulated, identical for the trophic level of each processed group of examination animal.Basal diet formula is in Table 1.The control group meat chick basal diet of feeding, test I group is added 400mg/kg armillaria mellea accreting with Rhizoma Gastrodiae powder on basal diet basis, test II group is added 800mg/kg sub-micron Chinese herbal medicine on basal diet basis, and test III group is added this product 0.2%(and substituted in basal diet 0.2% stone flour on basal diet basis).This product is the feed addictive that embodiment 1 prepares.
Table 1
Note: the rope premix of 1. supporting one's family (every kilogram of feed provides): rope A12500IU supports one's family; Calciferol 500IU; Be below milligram: vitamin e1 8.75, vitamin K
32.65, Cobastab
12, Cobastab
26, Cobastab
120.025, biotin 0.0325, folic acid 1.25, pantothenic acid 12, nicotinic acid 50;
2. microelement pre-mix material (per kilogram feed provides a milligram number): Cu8, Zn75, Fe80, Mn100, Se0.15, I0.35;
3. except metabolizable energy, result is calculated value; Later stage feed does not add any antibiotic, or with armillaria mellea accreting with Rhizoma Gastrodiae powder or astragalus polyose or zeolite powder.
Feeding and management and carry out immunity routinely.
Index determining: weigh and calculate daily ingestion amount, daily gain and feedstuff-meat ratio; Statistics survival rate; Butcher rear separating immune organ and calculate Immune Organs Index; After blood sampling, detect antibody titer of ND, PBLC conversion ratio, NKT (NK) cellular immune function and interleukin (IL) activity.
Data processing method: utilize SPSS(16.0) statistics software carries out variance analysis, adopts the least significant difference (LSD) to carry out multiple ratio.Data represent with average ± standard deviation (X ± SD).
Test result is as following table 2～table 7:
Table 2
Note: the female different person's significant differences of same column shoulder marking-up.
As shown in Table 2, from whole feeding period, compare with control group, the daily ingestion amount of test I group has improved 3.86%(p<0.05), daily gain has improved 5.99%(p<0.01), feedstuff-meat ratio has reduced 2.12%(p<0.05), meat chick livability has improved 5%; The daily ingestion amount of test II group has improved 2.17%(p<0.05), daily gain has improved 3.94%(p<0.05), feedstuff-meat ratio has reduced 1.59%(p<0.05), meat chick livability has improved 4%; The daily ingestion amount of test III group has improved 5.13%(p<0.01), daily gain has improved 8.48%(p<0.01), feedstuff-meat ratio has reduced 3.17%(p<0.01), meat chick livability has improved 6%.That is: in daily ration of broiler, add after feed addictive of the present invention, broiler chicken daily ingestion amount, daily gain and meat chick livability are significantly increased, and broiler chicken feedstuff-meat ratio significantly reduces.
Table 3
Note: the female different person's significant differences of colleague's shoulder marking-up.
As shown in Table 3,21d(21 days, lower same) time, compare with control group, the thymus index of test I group has improved 13.16%(p<0.01), index and spleen index has improved 20.41%(p<0.01), bursa of farbricius index has improved 13.23%(p<0.01); The thymus index of test II group has improved 10.53%(p<0.01), index and spleen index has improved 18.37%(p<0.01), bursa of farbricius index has improved 12.06%(p<0.01); The thymus index of test III group has improved 20.47%(p<0.01), index and spleen index has improved 28.57%(p<0.01), bursa of farbricius index has improved 22.57%(p<0.01).
During 42d, compare with control group, the thymus index of test I group has improved 18.69%(p<0.01), index and spleen index has improved 25%(p<0.01), bursa of farbricius index has improved 18.87%(p<0.01); The thymus index of test II group has improved 16.26%(p<0.01), index and spleen index has improved 21.05%(p<0.01), bursa of farbricius index has improved 17.61%(p<0.01); The thymus index of test III group has improved 27.68%(p<0.01), index and spleen index has improved 36.84%(p<0.01), bursa of farbricius index has improved 30.19%(p<0.01).
That is: in daily ration of broiler, add feed addictive of the present invention, after eaten by broilers 21d, 42d, the Immune Organs Indexes such as the thymus index of broiler chicken, index and spleen index and bursa of farbricius index all significantly strengthen.
Table 4
Note: the female different person's significant differences of colleague's shoulder marking-up.
As shown in Table 4, during 21d, compare with control group, the HI antibody titer of test I group has improved 26.49%(p<0.01), the HI antibody titer of test II group has improved 24.59%(p<0.01), the HI antibody titer of test III group has improved 37.03%(p<0.01).
During 42d, compare with control group, the HI antibody titer of test I group has improved 26.09%(p<0.01), the HI antibody titer of test II group has improved 25.06%(p<0.01), the HI antibody titer of test III group has improved 37.60%(p<0.01).
That is: in daily ration of broiler, add feed addictive of the present invention, after eaten by broilers 21d, 42d, the HI antibody titer of broiler chicken significantly increases.
Table 5
Note: the female different person's significant differences of colleague's shoulder marking-up.
As shown in Table 5, during 21d, compare with control group, the T lymphocyte transformation rate of test I group has improved 24.59%(p<0.01), bone-marrow-derived lymphocyte conversion ratio has improved 34.69%(p<0.01); The T lymphocyte transformation rate of test II group has improved 19.67%(p<0.01), bone-marrow-derived lymphocyte conversion ratio has improved 28.57%(p<0.01); The T lymphocyte transformation rate of test III group has improved 37.70%(p<0.01), bone-marrow-derived lymphocyte conversion ratio has improved 53.06%(p<0.01).
During 42d, compare with control group, the T lymphocyte transformation rate of test I group has improved 20.99%(p<0.01), bone-marrow-derived lymphocyte conversion ratio has improved 23.61%(p<0.01); The T lymphocyte transformation rate of test II group has improved 16.05%(p<0.01), bone-marrow-derived lymphocyte conversion ratio has improved 18.06%(p<0.01); The T lymphocyte transformation rate of test III group has improved 34.57%(p<0.01), bone-marrow-derived lymphocyte conversion ratio has improved 38.89%(p<0.01).
That is: in daily ration of broiler, add feed addictive of the present invention, after eaten by broilers 21d, 42d, the T lymphocyte transformation rate of broiler chicken and bone-marrow-derived lymphocyte conversion ratio all significantly increase.
Table 6
Note: the female different person's significant differences of colleague's shoulder marking-up.
As shown in Table 6, during 21d, compare with control group, the NK cytoactive of test I group has improved 32.89%(p<0.01), the NK cytoactive of test II group has improved 29.62%(p<0.01), the NK cytoactive of test III group has improved 51.25%(p<0.01).
During 42d, compare with control group, the NK cytoactive of test I group has improved 35.95%(p<0.05), the NK cytoactive of test II group has improved 27.90%(p<0.01), the NK cytoactive of test III group has improved 44.96%(p<0.01).
That is: in daily ration of broiler, add feed addictive of the present invention, after eaten by broilers 21d, 42d, the NK cytoactive of broiler chicken all significantly increases.
Table 7
Note: the female different person's significant differences of colleague's shoulder marking-up.
As shown in Table 7, during 21d, compare with control group, the IL-2 activity of test I group has improved 52.63%(p<0.01), IL-3 activity has improved 75%(p<0.01); The IL-2 activity of test II group has improved 42.11%(p<0.01), IL-3 activity has improved 56.25%(p<0.01); The IL-2 activity of test III group has improved 84.21%(p<0.01), IL-3 activity has improved 112.5%(p<0.01).
During 42d, compare with control group, the IL-2 activity of test I group has improved 66.67%(p<0.01), the active 92.86%(p<0.01 that improves of IL-3); The IL-2 activity of test II group has improved 55.56%(p<0.01), IL-3 activity has improved 78.57%(p<0.01); The IL-2 activity of test III group has improved 100%(p<0.01), IL-3 activity has improved 128.6%(p<0.01).
That is: in daily ration of broiler, add feed addictive of the present invention, after eaten by broilers 21d, 42d, the IL-2 of broiler chicken activity and IL-3 activity all significantly increase.
From above-mentioned test result, in daily ration of broiler, add after feed addictive of the present invention, to compare with control group, broiler chicken daily ingestion amount, daily gain significantly improve, and the death rate and feedstuff-meat ratio significantly decline; Immunity of meat chickens (comprising Immune Organs Index, HI antibody titer, periphery lymphocyte conversion ratio, NK cytoactive, IL activity index) significantly strengthens.
Claims (8)
1. a feed addictive that improves growth of meat chicken immune performance, is characterized in that, comprising: the Radix Astragali, folium isatidis, motherwort, hawthorn, Fructus Hordei Germinatus, Divine Comedy, pine needle meal, armillaria mellea accreting with Rhizoma Gastrodiae powder and carrier.
2. the feed addictive of raising growth of meat chicken immune performance according to claim 1, is characterized in that, according to percentage by weight, calculates, and comprising:
3. the feed addictive of raising growth of meat chicken immune performance according to claim 1, is characterized in that, according to percentage by weight, calculates, and comprising:
4. according to the feed addictive of the raising growth of meat chicken immune performance described in the arbitrary claim of claim 1～3, it is characterized in that, described carrier is zeolite powder.
5. prepare a method that improves the feed addictive of growth of meat chicken immune performance described in the arbitrary claim of claim 1～3, it is characterized in that, comprising:
(1) get required Chinese medicine, section, mixes, and mixed mixture is carried out to coarse crushing, then carries out cellular level pulverizing, and it is 25～40 microns that mixture is crushed to average grain diameter, adds first's carrier, mixes, and obtains sub-micron Chinese herbal medicine;
(2) the sub-micron Chinese herbal medicine obtaining and armillaria mellea accreting with Rhizoma Gastrodiae powder are mixed, then add second portion carrier, mix and obtain feed addictive.
6. an application process that improves the feed addictive of growth of meat chicken immune performance, is characterized in that, described feed addictive is the feed addictive of the raising growth of meat chicken immune performance described in the arbitrary claim of claim 1～3.
7. the application process of the feed addictive of raising growth of meat chicken immune performance according to claim 1, is characterized in that, described feed addictive is when reality is used, and addition is the 0.1-0.5% of feed weight.
8. the application process of the feed addictive of raising growth of meat chicken immune performance according to claim 1, is characterized in that described feed addictive is when reality is used, and addition is the 0.1-0.3% of feed weight.
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| CN104138498A (en) * | 2014-06-28 | 2014-11-12 | 四川农业大学 | Traditional Chinese medicine decoction composition for prompting chicken meat productivity and application thereof |
| CN104857376A (en) * | 2015-05-11 | 2015-08-26 | 安阳工学院 | Compound traditional Chinese medicine capable of promoting growing of broilers |
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| CN106360040A (en) * | 2016-08-24 | 2017-02-01 | 广东海大集团股份有限公司畜牧水产研究中心 | Composite Chinese herbal medicine and probiotics feed additive for improving growth property and immuno-functions of broiler chicken |
| CN107183397A (en) * | 2017-04-16 | 2017-09-22 | 苫君强 | Improve the preparation method of the feed addictive of quail immunologic function |
| CN110250357A (en) * | 2019-06-11 | 2019-09-20 | 云南农业大学 | A kind of Chinese herbal feed additive improving meat chicken growth performance |
| CN116870091A (en) * | 2023-07-13 | 2023-10-13 | 华南农业大学 | Traditional Chinese medicine compound for resisting LPS stress of broiler chickens as well as preparation and application thereof |
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| CN110250357A (en) * | 2019-06-11 | 2019-09-20 | 云南农业大学 | A kind of Chinese herbal feed additive improving meat chicken growth performance |
| CN116870091A (en) * | 2023-07-13 | 2023-10-13 | 华南农业大学 | Traditional Chinese medicine compound for resisting LPS stress of broiler chickens as well as preparation and application thereof |
| CN116870091B (en) * | 2023-07-13 | 2024-04-12 | 华南农业大学 | Traditional Chinese medicine compound for resisting LPS stress of broiler chickens as well as preparation and application thereof |
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