CN103431190A - Immunopotentiator for young portunus trituberculatus and application thereof - Google Patents
Immunopotentiator for young portunus trituberculatus and application thereof Download PDFInfo
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- CN103431190A CN103431190A CN2013102998427A CN201310299842A CN103431190A CN 103431190 A CN103431190 A CN 103431190A CN 2013102998427 A CN2013102998427 A CN 2013102998427A CN 201310299842 A CN201310299842 A CN 201310299842A CN 103431190 A CN103431190 A CN 103431190A
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Abstract
The invention discloses an immunopotentiator for a young portunus trituberculatus and an application thereof. The immunopotentiator is characterized by being prepared by evenly mixing the following raw materials in parts by weight: 10-15 parts of vitamin C, 5-10 parts of levamisole, 10-15 parts of astaxanthin, 10-20 parts of hypoxanthine nucleotide, 20-30 parts of beta-1,3-glucan, and 20-30 parts of seaweed polysaccharides. The mass percentage content of the immunopotentiator in a feeding feed is 0.06-0.08%. The immunopotentiator has the advantages that: the addition amount of the immunopotentiator in the feed is only 0.06-0.08%, thus the immunity and the disease resistance of the young portunus trituberculatus are significantly improved, so as to improve the survival rate of breeding portunus trituberculatus, wherein the survival rate can be increased by 25% or more, and the immune protection rate can reach 50% or more; and the immunopotentiator has no toxic or side effect, no residue and no drug resistance, also has no adverse effect on the feed palatability, has less addition amount, and can significantly improve the efficacy of the immunity and the disease resistance of the young portunus trituberculatus.
Description
Technical field
The present invention relates to a kind of crabs immune reinforcing agent, especially relate to immunopotentiator and application thereof that a kind of juvenile horse crab is used.
Background technology
In recent years, along with the popularization of the intensive cultural technique of crab class, and cause the cultivated crabs class incidence of disease greatly to increase; For reducing the cultivation loss, and use in a large number antibiotic, thereby causing causing the sick resistance to the action of a drug of bacterium strengthens; In addition owing to using antibiotic to cause the food-safety problem of cultivated crabs class to cause people's extensive concern in breeding process.In order to improve the resistance against diseases of cultivated crabs class, be necessary to develop the additive that improves crab para-immunity ability, the immunopotentiator be widely used at present mainly comprises Chinese herbal and crude drugs preparations, polysaccharide preparation, small peptide preparation and nutrition class preparation etc.The Chinese herbal and crude drugs preparations slow curative effect, the factor such as active constituents of medicine is uncertain and use limited.The polysaccharide preparation is as astragalus polyose, β-1,3 glucan, and the small peptide preparation is as not remarkable as curative effects such as bacitracins.Oligosaccharides is subject to extensive concern owing to can improving interleukin 8 quality in blood etc., current oligosaccharide kind preparation is 200810063203.X as Chinese Patent Application No., name is called a kind of feed addictive that strengthens immunity of sea water fish, disclose by Clostridium butylicum, reveal oligosaccharides, xylo-oligosaccharide and or the fish immunopotentiator that forms of FOS; Add this immunopotentiator 1% and can significantly improve the fish daily gain in feed, reduce feed coefficient.Chinese Patent Application No. is CNCN201010595637.1, and name is called the immunopotentiator that a kind of larval Pseudosciaena crocea is used, and disclosing by FOS, galactooligosacchari(es, manna oligosacchride and yeast extract is that the even mixed preparing of raw material forms; This immunopotentiator addition in feed is only 0.1～0.2%, just obviously improves immunity and the disease resistance of larval Pseudosciaena crocea.But the related immune reinforcing agent of not using for juvenile horse crab at present.
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Summary of the invention
Technical problem to be solved by this invention is to provide a kind of immunopotentiator used for juvenile horse crab; it is few that this immunopotentiator has an addition; can obviously improve immunity and the disease resistance of juvenile horse crab; the cultivation survival rate can improve 25% and more than, immune protective rate reaches the advantage more than 50%.
The present invention solves the problems of the technologies described above adopted technical scheme: the immunopotentiator that a kind of juvenile horse crab is used is formed by the even mixed preparing of the raw material of following portions by weight: vitamin C 10-15; Levamisol 5-10, astaxanthin 10-15, inosinic acid (IMP) 10-20, β-1,3-glucan 20-30, algal polysaccharides 20-30.
The immunopotentiator that above-mentioned juvenile horse crab is used, preferably formed by the even mixed preparing of the raw material of following portions by weight: vitamin C 12; Levamisol 8, astaxanthin 12, inosinic acid (IMP) 15, β-1,3-glucan 25, algal polysaccharides 25.
The application of the immunopotentiator that a kind of juvenile horse crab is used, the mass percentage content of this immunopotentiator in the feed of throwing something and feeding is 0.06-0.08%.
In the formula of above-mentioned immunopotentiator, vitamin C is biological anti-oxidant, has the juvenile horse crab premunition of raising and anti-stress ability; Levamisol is chemical synthesis class material, has the effect of regulating the juvenile horse crab immunologic function; Astaxanthin is chemical synthesis class material or extracts from marine alga and shellfish to have the function that improves the juvenile horse crab oxidation resistance and have immunoprotection; Inosinic acid has the immune response that improves juvenile horse crab, resists the function that various pathogen are invaded; Algal polysaccharides extracts in brown alga or red algae, and major function is to improve the immunity of cultivation juvenile horse crab; β-1, the 3-glucan is extracted by yeast cell wall, and major function is to improve the immunity of cultivation juvenile horse crab.This composite immune reinforcing agent is the factors such as vulnerable and environment and immunity reduction of causing etc. can significantly improve the growth of juvenile horse crab and the effect of survival rate for the juvenile horse crab shell phase.
Compared with prior art, the invention has the advantages that: the immunopotentiator that a kind of juvenile horse crab of the present invention is used, by vitamin C, levamisol, astaxanthin, inosinic acid, β-1,3-glucan, algal polysaccharides are that the even mixed preparing of raw material forms; This immunopotentiator addition in feed is only 0.06～0.08%, just obviously improve immunity and the disease resistance of juvenile horse crab, thereby improve the survival rate of Portunus trituberculatus Miers fry complete period cultivation, survival rate can improve 25% and more than, immune protective rate reaches more than 50%; And this immunopotentiator has no side effect, noresidue and without the resistance to the action of a drug, to the feed palatability also without any harmful effect.
In sum; immunopotentiator and application thereof that a kind of juvenile horse crab of the present invention is used, have an addition few, can obviously improve immunity and the disease resistance of juvenile horse crab; the cultivation survival rate can improve 25% and more than, immune protective rate reaches more than 50%.
The specific embodiment
Below in conjunction with embodiment, the present invention is described in further detail.
Embodiment 1
The immunopotentiator that a kind of juvenile horse crab is used, take respectively vitamin C 10 by weight percentage; Levamisol 5, astaxanthin 15, inosinic acid (IMP) 10, β-1,3-glucan 30, algal polysaccharides 30, evenly mix above-mentioned each raw material, is prepared into immunopotentiator.
In the formula of above-mentioned immunopotentiator, β-1, the do extract of beer yeast cells wall of 3-glucan, make by extracting yeast cell wall after supersonic wave wall breaking, β in yeast extract-1,3-glucan concentration is 20%.
Embodiment 2
The immunopotentiator that a kind of juvenile horse crab is used, substantially the same manner as Example 1, difference is in composition of raw materials: vitamin C 12; Levamisol 8, astaxanthin 12, inosinic acid (IMP) 15, β-1,3-glucan 25, algal polysaccharides 25, evenly mix above-mentioned each raw material, is prepared into immunopotentiator.
Embodiment 3
The immunopotentiator that a kind of juvenile horse crab is used, substantially the same manner as Example 1, difference is in composition of raw materials: vitamin C 15; Levamisol 10, astaxanthin 10, inosinic acid (IMP) 20, β-1,3-glucan 20, algal polysaccharides 20, evenly mix above-mentioned each raw material, is prepared into immunopotentiator.
Test example
Breed variety: 2.5 gram Portunus trituberculatus Miers juvenile crab 4000 tails, Random assignment is 4 groups, every group of 5 repetitions, each cement pit (specification is 3m * 1.5m * 1m, long * wide * dark)) cultivation Portunus trituberculatus Miers 200 tails, it is identical that initial body weight is respectively organized in experiment; Cultivating condition is as basic identical as environmental factor, chemical factor and biological factor.
Aquaculture management: other condition is basic identical, be added with 0.06%(weight in the different just cement pit feed of throwing something and feeding for 1 every day) immunopotentiator of the Portunus trituberculatus Miers of embodiment 1; Be added with 0.06%(weight in the feed that cement pit is thrown something and fed for 2 every days) immunopotentiator of the Portunus trituberculatus Miers of embodiment 2; Be added with 0.06%(weight in the feed that cement pit is thrown something and fed for 3 every days) immunopotentiator of the Portunus trituberculatus Miers of embodiment 3; Do not add the immunopotentiator of Portunus trituberculatus Miers in the feed that cement pit is thrown something and fed for 4 every days.
Result compares: after cultivating 8 weeks, calculate the Portunus trituberculatus Miers mantissa of each cement pit, be calculated to be motility rate; The all crabs of each cement pit are weighed simultaneously, calculate rate of body weight gain.Experimental result is as shown in table 1.As can be seen from Table 1, the high 25-30.5% of cement pit 1,2,3 survival rate, the immune protective rate of the immunopotentiator group of throwing something and feeding is at 51.2-62.2%.Illustrate that the anti-of Portunus trituberculatus Miers of the present invention stress have advantages of the survival rate that improves Portunus trituberculatus Miers and accelerate Growth of Portunus Trituberculatus speed by reinforcing agent.Immune protective rate [immune protective rate=(the 1-experimental group death rate/control group death rate) * 100].
Portunus trituberculatus Miers survival rate and the immune protective rate of each cement pit of table 1
| ? | Cement pit 1 | Cement pit 2 | Cement pit 3 | Cement pit 4 |
| Total mantissa | 152 | 163 | 156 | 102 |
| Survival rate % | 76.0 | 81.5 | 78.0 | 51 |
| Immune protective rate | 51.2 | 62.2 | 55.1 | 0 |
Above-described embodiment is to further illustrate of the present invention, but protection scope of the present invention is as the criterion with claims.
Claims (3)
1. the immunopotentiator that juvenile horse crab is used, is characterized in that being formed by the even mixed preparing of the raw material of following portions by weight: vitamin C 10-15; Levamisol 5-10, astaxanthin 10-15, inosinic acid (IMP) 10-20, β-1,3-glucan 20-30, algal polysaccharides 20-30.
2. the immunopotentiator that juvenile horse crab is used, is characterized in that being formed by the even mixed preparing of the raw material of following portions by weight: vitamin C 12; Levamisol 8, astaxanthin 12, inosinic acid (IMP) 15, β-1,3-glucan 25, algal polysaccharides 25.
3. the application of the immunopotentiator that a juvenile horse crab according to claim 1 is used, it is characterized in that: the mass percentage content of immunopotentiator claimed in claim 1 in the feed of throwing something and feeding is 0.06-0.08%.
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| CN201310299842.7A CN103431190B (en) | 2013-07-17 | 2013-07-17 | Immunopotentiator for young portunus trituberculatus and application thereof |
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| CN201310299842.7A CN103431190B (en) | 2013-07-17 | 2013-07-17 | Immunopotentiator for young portunus trituberculatus and application thereof |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103844139A (en) * | 2013-12-23 | 2014-06-11 | 浙江省海洋开发研究院 | Compound feed for cultivating soft-shell portunus trituberculatus and preparation method thereof |
| CN104273345A (en) * | 2014-08-14 | 2015-01-14 | 浙江省淡水水产研究所 | Complex immunity enhancer for Macrobra'chiumrosenbergiideman and application of complex immunity enhancer |
| CN104273333A (en) * | 2014-08-14 | 2015-01-14 | 浙江省淡水水产研究所 | Complex immunity enhancer for freshwater shrimps and application thereof |
| CN104872030A (en) * | 2015-06-16 | 2015-09-02 | 浙江海洋学院 | Babylonia areolata processing method |
| CN104872449A (en) * | 2015-06-15 | 2015-09-02 | 中国水产科学研究院黄海水产研究所 | Immunoenhancer for improving prawn quality |
| CN111743036A (en) * | 2020-06-24 | 2020-10-09 | 广东海洋大学 | Fish feed additive for improving antioxidant and immune functions |
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| CN101480488A (en) * | 2008-11-19 | 2009-07-15 | 广东海洋大学 | Complex immunopotentiator for grouper |
| CN101756026A (en) * | 2008-12-11 | 2010-06-30 | 天津瑞贝特科技发展有限公司 | Nutrient composition for enhancing shell hardening speed of shrimp and crab, preparation method and application thereof |
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Patent Citations (2)
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| CN101480488A (en) * | 2008-11-19 | 2009-07-15 | 广东海洋大学 | Complex immunopotentiator for grouper |
| CN101756026A (en) * | 2008-12-11 | 2010-06-30 | 天津瑞贝特科技发展有限公司 | Nutrient composition for enhancing shell hardening speed of shrimp and crab, preparation method and application thereof |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103844139A (en) * | 2013-12-23 | 2014-06-11 | 浙江省海洋开发研究院 | Compound feed for cultivating soft-shell portunus trituberculatus and preparation method thereof |
| CN104273345A (en) * | 2014-08-14 | 2015-01-14 | 浙江省淡水水产研究所 | Complex immunity enhancer for Macrobra'chiumrosenbergiideman and application of complex immunity enhancer |
| CN104273333A (en) * | 2014-08-14 | 2015-01-14 | 浙江省淡水水产研究所 | Complex immunity enhancer for freshwater shrimps and application thereof |
| CN104273345B (en) * | 2014-08-14 | 2019-03-12 | 浙江省淡水水产研究所 | A kind of Macrobrachium rosenbergii composite immune reinforcing agent and its application |
| CN104872449A (en) * | 2015-06-15 | 2015-09-02 | 中国水产科学研究院黄海水产研究所 | Immunoenhancer for improving prawn quality |
| CN104872030A (en) * | 2015-06-16 | 2015-09-02 | 浙江海洋学院 | Babylonia areolata processing method |
| CN111743036A (en) * | 2020-06-24 | 2020-10-09 | 广东海洋大学 | Fish feed additive for improving antioxidant and immune functions |
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| CN103431190B (en) | 2015-04-29 |
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