CN104666326A - Composition capable of improving immunity of fish body and preparation method of composition - Google Patents
Composition capable of improving immunity of fish body and preparation method of composition Download PDFInfo
- Publication number
- CN104666326A CN104666326A CN201310645540.0A CN201310645540A CN104666326A CN 104666326 A CN104666326 A CN 104666326A CN 201310645540 A CN201310645540 A CN 201310645540A CN 104666326 A CN104666326 A CN 104666326A
- Authority
- CN
- China
- Prior art keywords
- fish body
- composition
- compositions
- preparation
- parts
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Fodder In General (AREA)
Abstract
The invention relates to a composition capable of improving immunity of a fish body and a preparation method of the composition. The composition comprises the following components in parts by weight: 10-30 parts of amino acid, 10-20 parts of immune polysaccharides, 10-20 parts of levamisole hydrochloride, 5-30 parts of vitamin C and 10-20 parts of glucose. The composition provided by the invention is scientific in compatibility, simple in technology, convenient to use and relatively low in cost; the resistivity of the fish body can be effectively increased; the activity of an immune organ is increased; and the immune response of the organism is increased.
Description
Technical field
The invention belongs to field of veterinary, especially a kind of composition and method of making the same improving fish body immunity.
Background technology
Immunologic development is the thing of recent more than 30 years, nineteen sixty also only knew each different effect in the past, at the beginning of this century 1903 can being traced back to for the immunology history of fish, that year, Riegler found a kind ofly to make the fish of fourth eel to create agglutinating antibody at first, after this in more than 40 year, many experts and scholars are had progressively to have studied the immunological investigation of fish, the immune organs and tissues of Fish mainly contains spleen, thymus, lymphoid tissue, the environmental factors affecting fish immunity response mainly contains temperature, season and various water quality factors etc., within critical temperature, Fish antibody mediated immunity response intensity temperature raises and strengthens, and low temperature can enhancing non-specific immunity effect, granulocytic vigor and season closely related, water quality factors, as radioactive substance, what be dissolved in the water is organic, inorganic, the pollutant such as heavy metal ion, affect Fish body health, to the immunologic function of Fish, there is toxic action in various degree simultaneously.
Summary of the invention
The object of the present invention is to provide a kind of compositions improving fish body immunity, said composition overcomes the deficiencies in the prior art, and provide a kind of technique simple, easy to use, cost is lower.
Another object of the present invention is to provide a kind of preparation method improving the compositions of fish body immunity.
The present invention is achieved by the following technical solutions:
Improve a compositions for fish body immunity, component and the ratio of weight and number thereof of its compositions are respectively:
And said composition component and optimum ratio of weight and number thereof are respectively:
Improve a preparation method for the compositions of fish body immunity, the step of its preparation method is as follows:
(1) take aminoacid respectively according to weight proportion, immune polysaccharide, levamisole hydrochloride, vitamin C, glucose cross 65 mesh sieves;
(2) aminoacid after sieving, immune polysaccharide, levamisole hydrochloride, vitamin C, glucose are placed in the middle of three-dimensional mixer and carried out mixing 30 minutes, the compositions finished product of the fish body immunity that is namely improved.
Advantage of the present invention and beneficial effect are:
1. the present invention can promote propagation and the differentiation of target cell, strengthens infection and cell killing effect; Immune polysaccharide can affect the content of cytokine in blood circulation, also can affect the secretion of the lymphocyte cell factor of In vitro culture, and can promote the secretion of spleen lymphocyte interleukin, interferon, can also strengthen the effect of cytokine.
2. the present invention is except except direct killing and dissolution of bacteria or target cell and virus, also can have the opsonic action to immune complex, Chemotaxis Function and regulate the effect such as T, B cell immunity.
3. the present invention can increase in immunne response process and plays central role, and immune polysaccharide can strengthen T-lymphocyte function, promotes lymphocyte transformation and propagation.
Detailed description of the invention
The present invention is described in further detail by following examples.It should be noted that: following embodiment is illustrative, is not determinate, can not limit protection scope of the present invention with following embodiment.
Aminoacid involved in the present invention, immune polysaccharide, levamisole hydrochloride, vitamin C, glucose all commercially can be bought and obtain.
Embodiment 1
Improve a compositions for fish body immunity, component and the weight thereof of its compositions are respectively: 25 grams, aminoacid, immune polysaccharide 15 grams, levamisole hydrochloride 15 grams, Catergen 0 gram, glucose 15 grams.
Improve a preparation method for the compositions of fish body immunity, the step of its preparation method is as follows:
(1) take aminoacid respectively according to weight proportion, immune polysaccharide, levamisole hydrochloride, vitamin C, glucose cross 65 mesh sieves;
(2) aminoacid after sieving, immune polysaccharide, levamisole hydrochloride, vitamin C, glucose are placed in the middle of three-dimensional mixer and carried out mixing 30 minutes, the compositions finished product of the fish body immunity that is namely improved.
Embodiment 2
Improve a compositions for fish body immunity, component and the weight thereof of its compositions are respectively: 30 grams, aminoacid, immune polysaccharide 20 grams, levamisole hydrochloride 20 grams, vitamin C 30 grams, glucose 20 grams.
The step of its preparation method is with embodiment 1.
Embodiment 3
Improve a compositions for fish body immunity, component and the weight thereof of its compositions are respectively: amino acid/11 0 gram, immune polysaccharide 10 grams, levamisole hydrochloride 10 grams, vitamin C 50 grams, glucose 10 grams.
The step of its preparation method is with embodiment 1.
Clinical practice
1 materials and methods
1.1 material
1.1.1 the present invention is prepared by our company.
1.1.2 fish is tested: specification is the ill Cyprinus carpio of 200-350g.
1.1.3 feedstuff: certain feed companies 1012# Cyprinus carpio materials.
1.2 method
1.2.1 EXPERIMENTAL DESIGN
Sick for test fish is put into ready pond to support 10 temporarily, remove the serious sick fish of disease and dead fish, leave ill Cyprinus carpio 80 tail altogether, body weight is 200g-350g.Test fish to be assigned in No. 1,2, temporary curing cement pond in (1.6m × 1.6m × 0.8m) after sterilization.Wherein No. 1 pond is matched group, and No. 2 are of the present invention group.Feedstuff interpolation and medicine adding proportion are in table 1.
Table 1 matched group and of the present invention group of feedstuff adding proportion
| Group | 1 | 2 |
| Feedstuff master divide | Basestocks | Basestocks |
| Adding proportion | 0% | 1% |
| Daily ration of feeding | 3% | 3% |
After throwing something and feeding 7, the dead fish body in pond quantity during record cultivation, sick dissection fish body, determines hepatic infection situation.
1.2.2 blood sampling
Blood is got in docking, and the blood of every tail fish is divided into 2 parts, and wherein 1 part with anticoagulant heparin, prepares anticoagulation, active for detection leukocytes phagocytic.Another 1 part of blood is treated that it solidifies in first at room temperature static 1 hour, after keeping 4h to 4 DEG C of constant temperature refrigerators, under 4 DEG C of conditions with 4000r/min centrifugal 10 minutes, collects upper serum.
1.2.3 lysozyme (LSZ) determination of activity
Assay method, 15 tail test fishes are randomly drawed in 1, No. 2 pond, and get epidermal mucus, after homogenate, the centrifugal 10min of 2000r/min, gets supernatant test sample.Agar plate method is jointly adopted to measure with serum on blood.
1.2.4 leukocytes phagocytic situation measures
Add the S.aureus suspension that 0.1ml sterile saline is adjusted to 1 × 108 cells/ml in 0.2ml anticoagulant anticoagulation, put into 28 DEG C, hatch 1 hour, smear in thermostat water bath after shaking up, each blood sample is coated with 5 sheets, and naturally dry, methanol is fixed.Giemsa dyes 2 hours, and washing wind puts oily Microscopic observation counting, and presses formulae discovery percentage phagocytosis below.(phagocytic percentage, pp) and phagocytic index (phagocytic index, pi)
Cell number/100 of engulfing are participated in Pp=(100 leukocyte) × 100
Pi=is engulfed bacterial population/engulf bacterial cell number
2 results and analysis
2.1 fish body mortality contrasts
After 7 days, dead 3 tails in matched group pond, remain 37 tails, and after dissecting, liver is 34 tails of obvious bacterial infection symptom.Of the present invention group, dead 0 tail, remains 40 tails, and after dissecting, liver is obvious bacterial infection 1 tail.
2.2 lysozyme activity change contrasts
As shown in table 2, test group fish liver lysozyme activity of the present invention comparatively matched group is significantly increased, and proves that the present invention effectively can improve the lysozyme activity of fish body, improves fish body autoimmune ability.
Table 2 Cyprinus carpio lysozyme activity change contrast
2.3 leukocytic phagocytosis changes
As shown in table 3, after bait feeding on the 7th, the present invention effectively improves the phagocytic activity of Cyprinus carpio lymphocytes in blood and engulfs quantity, serves desirable defense reaction for extraneous thalline.
Table 3 leukocytes phagocytic rate and phagocytic index change list
| Group | Phagocytic rate (pp) | Phagocytic index (pi) |
| 1 | 33.71 | 3.08 |
| 2 | 50.22 | 5.46 |
Conclusion: the present invention has obvious immune enhancing function to the immune organ of Cyprinus carpio and phagocytic activity of leukocytes, is significantly improved to the immunologic function of body.
Claims (3)
1. improve a compositions for fish body immunity, it is characterized in that: component and the ratio of weight and number thereof of said composition are respectively:
2. the compositions of raising fish body immunity according to claim 1, is characterized in that: the optimum ratio of weight and number of said composition component is respectively:
3. the preparation method improving the compositions of fish body immunity as claimed in claim 1, is characterized in that: the step of this preparation method is as follows:
(1) take aminoacid respectively according to weight proportion, immune polysaccharide, levamisole hydrochloride, vitamin C, glucose cross 65 mesh sieves;
(2) aminoacid after sieving, immune polysaccharide, levamisole hydrochloride, vitamin C, glucose are placed in the middle of three-dimensional mixer and carried out mixing 30 minutes, the compositions finished product of the fish body immunity that is namely improved.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310645540.0A CN104666326A (en) | 2013-12-03 | 2013-12-03 | Composition capable of improving immunity of fish body and preparation method of composition |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310645540.0A CN104666326A (en) | 2013-12-03 | 2013-12-03 | Composition capable of improving immunity of fish body and preparation method of composition |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104666326A true CN104666326A (en) | 2015-06-03 |
Family
ID=53302264
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310645540.0A Pending CN104666326A (en) | 2013-12-03 | 2013-12-03 | Composition capable of improving immunity of fish body and preparation method of composition |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104666326A (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008011599A2 (en) * | 2006-07-20 | 2008-01-24 | Gourmetceuticals, Llc | Phosphorylated glucomannan polysaccharide for receptor mediated activation and maturation of monocyte-derived dendritic cells |
| CN101513231A (en) * | 2009-03-23 | 2009-08-26 | 厦门百拓生物工程有限公司 | Glycan additive premixing feed for aquatic animals |
| CN101664107A (en) * | 2008-09-04 | 2010-03-10 | 黄翔鹄 | High-efficiency shrimp immunomodifier |
| CN101757604A (en) * | 2008-12-11 | 2010-06-30 | 天津瑞贝特科技发展有限公司 | Immune polysaccharide intensifier and preparation method and application thereof |
| CN103211088A (en) * | 2013-05-08 | 2013-07-24 | 吕明涛 | Preparation method of sea cucumber bait |
| CN103271249A (en) * | 2013-06-14 | 2013-09-04 | 百洋水产集团股份有限公司 | Health-care fodder for freshwater fish |
-
2013
- 2013-12-03 CN CN201310645540.0A patent/CN104666326A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008011599A2 (en) * | 2006-07-20 | 2008-01-24 | Gourmetceuticals, Llc | Phosphorylated glucomannan polysaccharide for receptor mediated activation and maturation of monocyte-derived dendritic cells |
| CN101664107A (en) * | 2008-09-04 | 2010-03-10 | 黄翔鹄 | High-efficiency shrimp immunomodifier |
| CN101757604A (en) * | 2008-12-11 | 2010-06-30 | 天津瑞贝特科技发展有限公司 | Immune polysaccharide intensifier and preparation method and application thereof |
| CN101513231A (en) * | 2009-03-23 | 2009-08-26 | 厦门百拓生物工程有限公司 | Glycan additive premixing feed for aquatic animals |
| CN103211088A (en) * | 2013-05-08 | 2013-07-24 | 吕明涛 | Preparation method of sea cucumber bait |
| CN103271249A (en) * | 2013-06-14 | 2013-09-04 | 百洋水产集团股份有限公司 | Health-care fodder for freshwater fish |
Non-Patent Citations (1)
| Title |
|---|
| 宋航 等: "《制剂工程专业实验》", 28 February 2005 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109161515B (en) | Isolation culture method of primary hepatocytes of megalobrama amblycephala | |
| KR101709169B1 (en) | Anti-Bacterial Composition for Comprising Extract of Mixed Herbal Medication | |
| Ojerio et al. | Alginic acid as immunostimulant: effects of dose and frequency on growth performance, immune responses, and white spot syndrome virus resistance in tiger shrimp Penaeus monodon (Fabricius, 1798) | |
| Manayi et al. | Immunomodulation effect of aqueous extract of the artist's conk medicinal mushroom, Ganoderma applanatum (Agaricomycetes), on the rainbow trout (Oncorhynchus mykiss) | |
| de Oliveira David et al. | Fine structure of Mytella falcata (Bivalvia) gill filaments | |
| Mohan et al. | Isolation and characterization of Molluscan glycosaminoglycans from Pazhayar, South-East coast of India | |
| CN104666326A (en) | Composition capable of improving immunity of fish body and preparation method of composition | |
| JP2013188196A (en) | Virus infection prevention lactic acid bacterium composition and virus infection prevention lactic fermentation food | |
| CN103768205A (en) | Traditional Chinese medicine composition for treating freshwater fish bacterial liver-gall syndrome and preparation method of traditional Chinese medicine composition | |
| Wang et al. | Lentinan extracted from shiitake mushrooms (Lentinus edodes) improves the non-specific immunity of sea cucumber (Apostichopus japonicus) | |
| CN102551041A (en) | Refined seal oil, seal oil soft capsules and application of seal oil soft capsules in preparing health products for improving chemical liver injury | |
| CN116850086A (en) | Epicatechin hydrogel and preparation method and application thereof | |
| Benson et al. | Water-soluble egg membrane enhances the immunoactivating properties of an Aloe vera-based extract of Nerium oleander leaves | |
| Ofongo et al. | Effect of enzyme supplementation and plant extracts on villus height and microbial counts in broilers | |
| Ainsworth et al. | Effects of in vitro exposure to autologous blood and serum on expression of interleukin-8, interleukin-1β, and chemokine (CXC motif) ligand 2 in equine primary bronchial epithelial cell cultures | |
| CN113667630A (en) | In-vitro model and method for evaluating and screening traditional Chinese medicine extract for resisting inflammation and resisting oxidation of intestinal tract | |
| CN113278669A (en) | Silkworm chrysalis peptide and preparation and application thereof | |
| CN111568912A (en) | Glycyrrhizic acid and probiotics are combined to be used as feed additive for relieving vomitoxin harm | |
| Zhang et al. | Evaluation of the difference of L-selectin, tumor necrosis factor-α and sialic acid concentration in dairy cows with subclinical ketosis and without subclinical ketosis. | |
| CN108967667B (en) | Application of chitosan oligosaccharide in pelteobagrus fulvidraco feed additive | |
| Ortizo et al. | Plasma biochemistry levels and hematological parameters in Mallard ducks (Anas platyrhynchos Linn.) from selected semi-free range duck farms in Misamis Occidental and Zamboanga Del Sur, Philippines. | |
| CN106616029B (en) | Feed additive for improving disease resistance of rainbow trout and preparation method thereof | |
| CN111643518B (en) | Fishery immunopotentiator and application thereof | |
| CN105341507B (en) | Disease-resistant immunopotentiator cyclo- (alanine-tryptophan) for snakeheads | |
| Foekh et al. | The ability of earthworm Lumbricus rubellus extract in slowing down the activation of NFkB and TNF-αin lipopolysaccharide-induced Rattus norvegicus |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20150603 |
|
| WD01 | Invention patent application deemed withdrawn after publication |