CN103768205A - Traditional Chinese medicine composition for treating freshwater fish bacterial liver-gall syndrome and preparation method of traditional Chinese medicine composition - Google Patents
Traditional Chinese medicine composition for treating freshwater fish bacterial liver-gall syndrome and preparation method of traditional Chinese medicine composition Download PDFInfo
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- CN103768205A CN103768205A CN201210413426.0A CN201210413426A CN103768205A CN 103768205 A CN103768205 A CN 103768205A CN 201210413426 A CN201210413426 A CN 201210413426A CN 103768205 A CN103768205 A CN 103768205A
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Abstract
The invention relates to a traditional Chinese medicine composition for a treating freshwater fish bacterial liver-gall syndrome. The traditional Chinese medicine composition comprises the following components by weight: 20 to 30 parts of oriental wormwood, 2 to 3 parts of immature bitter orange, 5 to 10 parts of radix hedysari, 5 to 10 parts of fructus forsythiae, 15 to 20 parts of rheum officinale and 5 to 10 parts of cape jasmine. The traditional Chinese medicine composition aims to improve the lysozyme activity in the freshwater fish blood and tissue and the phagocytic ability of lymphocytes, and improve the immunity of the organism, so that the bacterial liver-gall syndrome caused by streptococcus, vibrio and the like in recent years can be treated. The natural herbal medicines are performed natural airdrying, smashed to more than 80 mesh, and is uniformly mixed with fish feed to feed, the use is convenient, and toxic and side effects can be avoided.
Description
Technical field:
The invention belongs to Aquatic product field of veterinary,, especially a kind of Chinese medicine composition for the treatment of fresh water fish bacterial liver and gallbladder syndrome and preparation method thereof.
Background technology:
In recent years, because cultivation density is too high, feedstuff feeding is too much, causes fish body nutrient imbalance, and body constitution declines, and secondary infection antibacterial, causes that the case of bacillary liver and gallbladder syndrome can be found everywhere.Such disease is because treatment cycle is longer, and the conventional antimicrobial of tradition not yet reaches ideal effect and will produce drug resistance, and therefore treatment exists larger difficulty.And Chinese herbal medicine is because it possesses the fish of raising body self lysozyme activity, increases the cytophagous feature of engulfing ratio, strengthening the immunocompetence of ill fish body self, treat such disease from the disease-resistant angle of fish body self, change the outside antibacterial Therapeutic Method of conventional medicament, just can overcome the blind area of traditional drug for such disease treatment, and Chinese medicine, compared with traditional antimicrobial, possesses the features such as conditioning, treatment are more comprehensive, no drug residue.
Summary of the invention:
The object of the present invention is to provide a kind of Chinese medicine composition for the treatment of fresh water fish bacterial liver and gallbladder syndrome, this Chinese medicine composition can improve lysozyme activity and lymphocytic phagocytic activity in fresh-water fishes body blood and tissue, improve immunity of organisms, and can there is not drug residue, easy to use, cost is lower.
Another object of the present invention is to provide a kind of preparation method of Chinese medicine composition for the treatment of fresh water fish bacterial liver and gallbladder syndrome.
The present invention is achieved through the following technical solutions:
Treat a Chinese medicine composition for fresh water fish bacterial liver and gallbladder syndrome, its Chinese medicine composition component and ratio of weight and number thereof are respectively:
Herba Artemisiae Scopariae 20-30 part;
Fructus Aurantii Immaturus 2-3 part;
Radix Hedysari 5-10 part;
Fructus Forsythiae 5-10 part;
Radix Et Rhizoma Rhei 15-20 part;
Fructus Gardeniae 5-10 part.
And the component of this Chinese medicine composition and optimum ratio of weight and number thereof are:
18 parts of Herba Artemisiae Scopariaes;
3 parts of Fructus Aurantii Immaturuss;
10 parts of Radix Hedysari;
8 parts of Fructus Forsythiaes;
17 parts of Radix Et Rhizoma Rhei;
8 parts of Fructus Gardeniaes.
Treat a preparation method for the Chinese medicine composition of fresh water fish bacterial liver and gallbladder syndrome, its step is as follows:
(1) take respectively Herba Artemisiae Scopariae, Fructus Aurantii Immaturus, Radix Hedysari, Fructus Forsythiae, Radix Et Rhizoma Rhei, Fructus Gardeniae according to weight proportion and pulverize, and respectively all by 80 mesh sieves;
(2) by after sieving Herba Artemisiae Scopariae, Fructus Aurantii Immaturus, Radix Hedysari, Fructus Forsythiae, Radix Et Rhizoma Rhei, Fructus Gardeniae put in three-dimensional hybrid tank fully mix homogeneously, the Chinese medicine composition finished product of the fresh water fish bacterial liver and gallbladder syndrome that obtains medical treatment.
Advantage of the present invention and beneficial effect are:
1, the present invention can improve lysozyme activity and lymphocytic phagocytic activity in fresh-water fishes body blood and tissue, improves immunity of organisms,, go to resist disease by autoimmune system, thus the bacillary liver and gallbladder syndrome that treatment causes due to streptococcus, vibrio etc.
2, the present invention adopts natural Chinese medicines composition, little for the damage of fish body, drug residue free.
The specific embodiment
The present invention is described in further detail by following examples.It should be noted that: following embodiment is illustrative, is not determinate, can not limit protection scope of the present invention with following embodiment.
In component involved in the present invention, Herba Artemisiae Scopariae, Fructus Aurantii Immaturus, Radix Hedysari, Fructus Forsythiae, Radix Et Rhizoma Rhei, Fructus Gardeniae all can be bought and obtain on market.
Embodiment 1
Treat a Chinese medicine composition for fresh water fish bacterial liver and gallbladder syndrome, component and the weight thereof of its Chinese medicine composition are respectively: Herba Artemisiae Scopariae 20g, Fructus Aurantii Immaturus 2g, Radix Hedysari 5g, Fructus Forsythiae 5g, Radix Et Rhizoma Rhei 15g, Fructus Gardeniae 5g.
Treat a preparation method for the Chinese medicine composition of fresh water fish bacterial liver and gallbladder syndrome, the step of preparation method is as follows:
(1) take respectively Herba Artemisiae Scopariae, Fructus Aurantii Immaturus, Radix Hedysari, Fructus Forsythiae, Radix Et Rhizoma Rhei, Fructus Gardeniae according to weight proportion and pulverize, and respectively all by 80 mesh sieves;
(2) by after sieving Herba Artemisiae Scopariae, Fructus Aurantii Immaturus, Radix Hedysari, Fructus Forsythiae, Radix Et Rhizoma Rhei, Fructus Gardeniae put in three-dimensional hybrid tank fully mix homogeneously, the Chinese medicine composition finished product of the fresh water fish bacterial liver and gallbladder syndrome that obtains medical treatment.
Embodiment 2
Treat a Chinese medicine composition for fresh water fish bacterial liver and gallbladder syndrome, component and the weight thereof of its Chinese medicine composition are respectively: Herba Artemisiae Scopariae 18g, Fructus Aurantii Immaturus 3g, Radix Hedysari 10g, Fructus Forsythiae 8g, Radix Et Rhizoma Rhei 17g, Fructus Gardeniae 8g.
The step of its preparation method is with embodiment 1.
Embodiment 3
Treat a Chinese medicine composition for fresh water fish bacterial liver and gallbladder syndrome, component and the weight thereof of its Chinese medicine composition are respectively: Herba Artemisiae Scopariae 30g, Fructus Aurantii Immaturus 3g, Radix Hedysari 10g, Fructus Forsythiae 10g, Radix Et Rhizoma Rhei 20g, Fructus Gardeniae 10g.
The step of its preparation method is with embodiment 1.
The using method of the Chinese medicine composition of this treatment fresh water fish bacterial liver and gallbladder syndrome, by 1% interpolation and fish meal evenly mix throw something and feed can reach treatment disease effect.
Clinical practice
1 materials and methods
1.1 material
1.1.1 treating the Chinese medicine composition of fresh water fish bacterial liver and gallbladder syndrome is prepared by our company.
1.1.2 test fish: specification is the ill Cyprinus carpio of 200-350g.
1.1.3 feedstuff: the Tong Wei feedstuff 1012# of group Cyprinus carpio materials.
1.2 method
1.2.1 EXPERIMENTAL DESIGN
Sick test fish is put into ready pond and support 10 temporarily, remove sick fish and dead fish that disease is serious, leave altogether ill Cyprinus carpio 80 tails, body weight is 200g-350g.Test fish is assigned in No. 1,2, temporary curing cement pond in (1.6m × 1.6m × 0.8m) after sterilization.Wherein No. 1 pond is matched group, is for No. 2 test group of the present invention.Feedstuff interpolation and medicine adding proportion are in table 1.
Table 1 matched group and test group feedstuff adding proportion of the present invention
| Group | 1 | 2 |
| Feedstuff master divide | Basestocks | Basestocks |
| Chinese medicine adding proportion | 0％ | 1％ |
| Daily ration of feeding | 3％ | 3％ |
Throw something and feed after 7 days, the dead fish body in pond quantity during record cultivation, the sick fish body of dissecting, determines liver infection conditions.
1.2.2 blood sampling
Blood is got in docking, and the blood of every tail fish is divided into 2 parts, and wherein 1 part with anticoagulant heparin, prepares anticoagulation, for detecting leukocytes phagocytic activity.Another 1 part of blood is treated to it solidifies in first at room temperature static 1 hour, keep after 4h to 4 ℃ of constant temperature refrigerators, under 4 ℃ of conditions with 4000r/min centrifugal 10 minutes, collect upper serum.
1.2.3 lysozyme (LSZ) determination of activity
Assay method, 1, No. 2 15 tail test fishes are randomly drawed in pond, get epidermal mucus, and after homogenate, the centrifugal 10min of 2000r/min, gets supernatant test sample.Jointly adopt agar plate method to measure with serum on blood.
1.2.4 leukocytes phagocytic situation is measured
In 0.2ml anticoagulant anticoagulation, add the S.aureus suspension that 0.1ml sterile saline is adjusted into every milliliter, 1 × 108 cell, after shaking up, put into 28 ℃, in thermostat water bath, hatch 1 hour, smear, each blood sample is coated with 5 sheets, naturally dries, and methanol is fixed.Giemsa dyeing 2 hours, washing wind is put oily Microscopic observation counting, and by the percentage phagocytosis of formula calculating below.(phagocytic percentage, pp) and phagocytic index (phagocytic index, pi)
Pp=(cell number/100 that in 100 leukocyte, participation is engulfed) × 100
Pi=is engulfed bacterial population/engulf bacterial cell number
2 results and analysis
2.1 fish body mortality contrasts
After 7 days, dead 3 tails in matched group pond, residue 37 tails, after dissecting, liver is 34 tails of obvious bacterial infection symptom.Test group of the present invention, dead 0 tail, residue 40 tails, after dissecting, liver is obvious bacterial infection 1 tail.
2.2 lysozyme activities change contrast
As shown in table 2, test group fish liver lysozyme activity of the present invention is significantly increased compared with matched group, proves that Chinese drug-treated group can effectively improve the lysozyme activity of fish body, improves fish body autoimmune ability.
Table 2 Cyprinus carpio lysozyme activity changes contrast
2.3 leukocytic phagocytosises change
As shown in table 3, after bait feeding on the 7th, Chinese prescription has effectively improved the phagocytic activity of Cyprinus carpio blood medium-sized lymphocyte and has engulfed quantity, has played desirable defense reaction for extraneous thalline.
Table 3 leukocytes phagocytic rate and phagocytic index change list
| Group | Phagocytic rate (pp) | Phagocytic index (pi) |
| 1 | 32.73 | 3.07 |
| 2 | 49.22 | 5.36 |
3 practice tests
Wide sharp breeding cultivation base, in June, 2012 Henan, main cultivation fresh water Siberia sturgeon, bacillary liver and gallbladder syndrome is serious, do not having in effective situation by antibiotic and quinolones, use the present invention to throw something and feed 7 days according to 1% ratio of throwing something and feeding, mortality drops to 13 tails from single cylinder tail every days 70, substantially stops death after 13 days.Randomly draw fish body 20 tails, obvious 1 tail of the symptoms of liver-gallbladder after dissecting, good 17 tails, still have 3 tails in the tissue repair phase, and visible the present invention's treatment is because the bacillary liver and gallbladder syndrome effect that streptococcus, vibrio etc. cause is fairly obvious.
Claims (3)
1. treat the Chinese medicine composition of fresh water fish bacterial liver and gallbladder syndrome for one kind.It is characterized in that: this Chinese medicine composition component and ratio of weight and number thereof are respectively:
Herba Artemisiae Scopariae 20-30 part;
Fructus Aurantii Immaturus 2-3 part;
Radix Hedysari 5-10 part;
Fructus Forsythiae 5-10 part;
Radix Et Rhizoma Rhei 15-20 part;
Fructus Gardeniae 5-10 part.
2. the Chinese medicine composition for the treatment of fresh water fish bacterial liver and gallbladder syndrome according to claim 1, is characterized in that: the component of this Chinese medicine composition and optimum ratio of weight and number thereof are:
18 parts of Herba Artemisiae Scopariaes;
3 parts of Fructus Aurantii Immaturuss;
10 parts of Radix Hedysari;
8 parts of Fructus Forsythiaes;
17 parts of Radix Et Rhizoma Rhei;
8 parts of Fructus Gardeniaes.
3. a Chinese medicine composition for treatment fresh water fish bacterial liver and gallbladder syndrome as claimed in claim 1, is characterized in that: the step of this preparation method is as follows:
(1) take respectively Herba Artemisiae Scopariae, Fructus Aurantii Immaturus, Radix Hedysari, Fructus Forsythiae, Radix Et Rhizoma Rhei, Fructus Gardeniae according to weight proportion and pulverize, and respectively all by 80 mesh sieves;
(2) by after sieving Herba Artemisiae Scopariae, Fructus Aurantii Immaturus, Radix Hedysari, Fructus Forsythiae, Radix Et Rhizoma Rhei, Fructus Gardeniae put in three-dimensional hybrid tank fully mix homogeneously, the Chinese medicine composition finished product of the fresh water fish bacterial liver and gallbladder syndrome that obtains medical treatment.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104998195A (en) * | 2015-07-15 | 2015-10-28 | 湖南康易达绿茵科技有限公司 | Traditional Chinese medicine for treating fish liver-gallbladder syndrome |
| CN108815464A (en) * | 2018-08-13 | 2018-11-16 | 成都通威三新药业有限公司 | A kind of Traditional Chinese drug mixture that treating aquatic fish liver and gall syndrome and preparation method |
| DE202023100017U1 (en) | 2023-01-03 | 2023-02-17 | Ravi Dhondiraj Barde | Novel drug for the prevention and treatment of bacterial mixed infections in freshwater fish |
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| JPS6475422A (en) * | 1987-09-17 | 1989-03-22 | Nisshin Flour Milling Co | Preventive and remedy for furunculosis of fishes |
| CN101961391A (en) * | 2010-06-23 | 2011-02-02 | 天津生机集团股份有限公司 | Traditional Chinese medicine composition for preventing and controlling grass carp disease |
| CN102018137A (en) * | 2010-10-27 | 2011-04-20 | 西北农林科技大学 | Fish liver injury-resistant Chinese herb additive and preparation method thereof |
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2012
- 2012-10-24 CN CN201210413426.0A patent/CN103768205A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6475422A (en) * | 1987-09-17 | 1989-03-22 | Nisshin Flour Milling Co | Preventive and remedy for furunculosis of fishes |
| CN101961391A (en) * | 2010-06-23 | 2011-02-02 | 天津生机集团股份有限公司 | Traditional Chinese medicine composition for preventing and controlling grass carp disease |
| CN102018137A (en) * | 2010-10-27 | 2011-04-20 | 西北农林科技大学 | Fish liver injury-resistant Chinese herb additive and preparation method thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104998195A (en) * | 2015-07-15 | 2015-10-28 | 湖南康易达绿茵科技有限公司 | Traditional Chinese medicine for treating fish liver-gallbladder syndrome |
| CN108815464A (en) * | 2018-08-13 | 2018-11-16 | 成都通威三新药业有限公司 | A kind of Traditional Chinese drug mixture that treating aquatic fish liver and gall syndrome and preparation method |
| DE202023100017U1 (en) | 2023-01-03 | 2023-02-17 | Ravi Dhondiraj Barde | Novel drug for the prevention and treatment of bacterial mixed infections in freshwater fish |
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Application publication date: 20140507 |