CN105559089A - Liver-protecting and toxin-expelling fruit and vegetable enzyme and preparation method thereof - Google Patents
Liver-protecting and toxin-expelling fruit and vegetable enzyme and preparation method thereof Download PDFInfo
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- CN105559089A CN105559089A CN201610076958.8A CN201610076958A CN105559089A CN 105559089 A CN105559089 A CN 105559089A CN 201610076958 A CN201610076958 A CN 201610076958A CN 105559089 A CN105559089 A CN 105559089A
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- 239000002689 soil Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 235000013599 spices Nutrition 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000000153 supplemental effect Effects 0.000 description 1
- 239000006188 syrup Substances 0.000 description 1
- 235000020357 syrup Nutrition 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- 230000004614 tumor growth Effects 0.000 description 1
- 235000019155 vitamin A Nutrition 0.000 description 1
- 239000011719 vitamin A Substances 0.000 description 1
- 235000019156 vitamin B Nutrition 0.000 description 1
- 239000011720 vitamin B Substances 0.000 description 1
- 235000021468 vitamin B8 Nutrition 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- 229940045997 vitamin a Drugs 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a liver-protecting and toxin-expelling fruit and vegetable enzyme and a preparation method thereof. The method includes the following steps of firstly, smashing fruit and vegetables; secondly, adding cane sugar, chlorella, enzyme activity-keeping agent and probiotics to the smashed fruit and vegetables to be sealed and fermented; thirdly, conducting filtering; fourthly, conducting sealing and standing. The enzyme contains main components including various edible enzymes, trace elements, minerals, vitamins, amino acid, protein, carbohydrate and the like; particularly, after the enzyme activity-keeping agent is used, the activity of the enzyme is protected, various outside-cell enzymes are complete, and activity is strong. The enzyme can adjust absorption and metabolism of bodies for individual physiques and compensate for the most comprehensive nutrients, and can effectively protect livers and expel toxin.
Description
Technical field
The present invention relates to nutraceutical field, be specifically related to a kind of protecting liver and expelling toxin pectase and preparation method thereof.
Background technology
Ferment is also called enzyme, is a class biocatalyst, and majority is protein, also has some to be RNA or DNA molecular.Containing thousands of kinds of enzymes in organism, they dominate many catalytic process such as biological metabolism, nutrition and power conversion, and the reaction in close relations with life process is enzymic catalytic reaction mostly, but enzyme not necessarily only plays catalytic action in cell.
In present stage, modern society's dog-eat-dog, rhythm of life is accelerated, and environmental pollution is day by day serious, and along with the growth at people's age, the ferment in people's body can reduce day by day.The minimizing of ferment, the appearance of various symptom will be caused, due to zymosthenic unicity, want these diseases of prevention and therapy, we must absorb more ferment from meals, and food is after high temperature, fine finishining, makes again the ferment in food be destroyed, the ferment causing people to absorb from food is very low, cannot meet the normal need of people.For making enzyme food more meet modern to diversity, the balance of nutritional need be easy to absorbability, the making of ferment has become instant topic.
Enzyme microb is with one or more raw materials such as vegetables or fruit, the functional microbial fermented food being rich in the nutritional labelings such as vitamin, enzyme, mineral matter and secondary metabolite produced through the fermentation of multiple beneficial bacterium.Enzyme microb has protecting liver and expelling toxin, delays senility, anti-inflammation, purify the blood, strengthen the plurality of health care functions such as body immunity and detoxicating and fighting cancer.
Therefore, provide one never containing artificial color, chemical spices and anticorrisive agent, it is necessary for having the fruit ferment that enzyme activity is high, protecting liver and expelling toxin is effective.
Summary of the invention
For above shortcomings in prior art, one of technical problem to be solved by this invention is to provide a kind of preparation method of protecting liver and expelling toxin pectase.
Two of technical problem to be solved by this invention is to provide protecting liver and expelling toxin pectase prepared by said method.
The present invention seeks to be achieved through the following technical solutions:
A preparation method for protecting liver and expelling toxin pectase, comprises the following steps:
(1) fruits and vegetables are pulverized;
(2) in the fruits and vegetables after pulverizing, sucrose, chlorella, enzyme live keeping agent, probio is added, sealing, fermentation;
(3) filter;
(4) sealing and standing.
Fruits and vegetables in described step (1), are made up of the raw material of following weight parts: red common beet 50-70 part, pineapple 40-60 part, carrot 5-15 part, apple 5-15 part, mulberry fruit 5-15 part, red date 5-15 part, agropyron 5-15 part.
In described step (2), sucrose addition is the 20-40wt% of fruits and vegetables gross weight; chlorella addition is the 2-12wt% of fruits and vegetables gross weight; enzyme live keeping agent addition is the 0.5-3.5wt% of fruits and vegetables gross weight, and probio addition is the 0.5-1.5wt% of fruits and vegetables gross weight.
Preferably, described enzyme live keeping agent is mixed by maltitol, hydroxyl isomaltulose, sweet mellow wine, and the mass ratio of described maltitol, hydroxyl isomaltulose, sweet mellow wine is (1-3): (1-3): (1-3).
Preferably, described probio is mixed by monascus ruber, aspergillus oryzae, Bifidobacterium, candida utili, and the mass ratio of described monascus ruber, aspergillus oryzae, Bifidobacterium, candida utili is (1-3): (1-3): (1-3): (1-3).
Preferably, in described step (2), the temperature of fermentation is 35-41 DEG C, and the time is 140-210 days.
Preferably, described step (3) is filtered and is used plate and frame filter to filter 1-3 time, and adopt 60-120 object filter cloth, controlled pressure is 0.1-0.5Mpa.
Preferably, described step (4) the sealing and standing time is 85-145 days.
Present invention also offers a kind of protecting liver and expelling toxin pectase, adopt said method to be prepared from.
Concrete, in the present invention:
Maltitol, No. CAS: 585-88-6.
Hydroxyl isomaltulose, No. CAS: 534-73-6.
Sweet mellow wine, No. CAS: 69-65-8.
Sucrose, No. CAS: 57-50-1.Preferably, sugarcane raw sugar.Sugarcane raw sugar take sugarcane as raw material, through pressure extracting juice, clarifying syrup process, boiling-off crystallization, centrifugal purging, make with one deck molasses; Its pol (pol) is not less than 97%.
Red common beet, i.e. beet root.Original name: beet, another name: Chard dish, formal name used at school: BetavulgarisL., English name: beetroot, Chenopodiaceae, Beta biennial herb, root is coniform to fusiform, succulence.Contained mineral compound and plant compound are that it is distinctive, and these compounds can be anti-infective, increase cell oxygen content, treatment blood disease, hepatopathy and immune system dysfunction; There are abundant potassium, phosphorus, sodium, iron, magnesium, sugar and vitamin A, B, C and B8 (Biotin) can excite insulin secretion to strengthen apple sugar to join and can help to digest betaine and can accelerate choleresis to help dredging liver blood vessel to block zinc enzyme plain.Containing to the extraordinary folic acid of human body, prevent anaemia, one of important substance making cell normal development indispensable.As according to there being certain antitumor action, belonging to a kind of natural anti-cancer food, and preventing hypertension, senile dementia in addition, the effect reduced blood pressure.
Agropyron, formal name used at school: Wheatgrass, Roegneria C. Koch is a Plants, is a kind of wheat of uniqueness, preferably complete agropyron.Containing a kind of SOD enzyme with the special ion such as zinc, copper, and content is up to 0.1%.SOD has special curative effect for the inflammation such as collagen disease, rhinitis, pleurisy of arthritis, iuntercellular tissue inflammation.There is the function of purification and activation kidney and liver cell.In the research of doctor to agropyron, have also discovered the abscisic acid of energy reversing tumor growth.Agropyron is the known unique method that can obtain a large amount of abscisic acid.
Apple, formal name used at school: MaluspumilaMill.Apple belongs to the rose family, deciduous tree, and elliptic leaf shape, has sawtooth; Fruit is spherical, and taste is sweet, and being common fruit, is also modal fruit, is rich in abundant nutrition.
Pineapple, formal name used at school: Ananascomosus is one of famous tropical fruit (tree).Fujian and Taiwan, area are referred to as pineapple, and Singapore and Malaysia one band is called yellow pears, and continent and Hongkong are called pineapple.Pineapple is nutritious, containing a large amount of fructose, and apple sugar, Cobastab, C, phosphorus, the material such as citric acid and protease.Containing multiple organic acid and bromelain, containing a kind of material being " pineapple proteinase ", its energy decomposing protein, help digest, dissolve the fibrin and blood clot that block in tissue, improve the blood circulation of local, dilution blood fat, diminishes inflammation and oedema, can promote blood circulation.To ephritis and hyperpietic useful, also have auxiliary curative effect to bronchitis.
Carrot, formal name used at school: Daucuscarota.Also known as sweet Xun, be Umbelliferae carrot, belong to biennial herb plant, do vegetables with fleshy root and eat.
Mulberry fruit, formal name used at school: FructusMori is the fruit of Moraceae Morus perennial woody plant mulberry tree, oval, long 1-3 centimetre, and air spots is sliding.Being green during prematurity, gradually growing up and become white, redness, is aubergine or atropurpureus after ripe, sweet and sour.Normal food mulberry fruit can improving eyesight, the symptom that relieving eye strain is dry and astringent.It has immunologic enhancement, has effect of gain to spleen, has humidification to hemolytic reaction, can prevent human body artery sclerosis, skeletal joint sclerosis, enhance metabolism.It can promote the growth of erythrocyte, prevents Neuroleptic Leukocytopenia, and has auxiliary effect to illnesss such as treatment diabetes, anaemia, hypertension, high fat of blood, coronary heart disease, neurasthenia.
Red date, formal name used at school: Ziziphuszizyphus, has another name called date.Feature is that vitamin content is very high, has the good reputation of " natural complex ball ", has nourishment for vitality, the effect of enriching blood.Red date is temperate crop, strong adaptability.
Chlorella, formal name used at school: Chlorellavulgaris, Chlorophyceae, chlorella section.Single-cell algae, Chang Dansheng, also has many cells to assemble.Cell is spherical, oval, inside has the chromatoplast of a Zhousheng, cup-shaped or sheet.In Europe, a lot of country has confirmed that chlorella is the medicable pollution-free food of tool.Chlorella is applicable to baby, pregnant woman, men and women, old and young completely, is best nutritional food that is safe, reliable, that have no side effect.The T cell activation in organism in immunocyte can be made, effectively bring back to life immunologic function, extend the life of cancer patient.Pneumonocyte fibrillatable can be prevented, prevention lung cancer; The organic chemical pollutant assembled in effective eliminating human body and heavy metal, and have no side effect.The teenager in growing, children's best green organic active nutritional food.In Japan, chlorella has been applied to clinical, radiation proof, the malnutrition caused wound, constipation, Neuroleptic Leukocytopenia, hypoferric anemia, juvenile's partial eclipse, hypertension, diabetes, atherosclerotic and hypercholesterolemia and tumour etc. carry out supplemental treatment.
Aspergillus oryzae, formal name used at school: Aspergillusoryzae.Aspergillus oryzae belongs to Deuteromycotina, Hyphomycetes, hyphomycetales, from Geng Bao section, and a Common Species in aspergillus fungi.Aspergillus oryzae is the bacterial strain that a class produces complex enzyme, except product protease, also can produce more than the 700 kind of enzymes such as amylase, carbohydrase, cellulase, phytase.Under diastatic effect, the straight chain in raw material, amylopectin are degraded to dextrin and various low molecule carbohydrate, as maltose, apple sugar etc.; Under the effect of protease, stodgy macro-molecular protein is degraded to peptone, polypeptide and each seed amino acid, and the mass degradation that in auxiliary material, the difficulty such as crude fibre, phytic acid absorbs can be made, improve nutritive value, health-care efficacy and digestibility, and applied more than 1000 year safely.
Monascus ruber, formal name used at school: monascuspurpureus, monascus ruber is a kind of fungi useful to the mankind, and monascus ruber can make rice fermentation be prepared from red yeast rice.Red colouring agent for food, also used as a Chinese medicine claims red bent ancient times, and being Chinese medicine, is again food.Red colouring agent for food, also used as a Chinese medicine have help digestion invigorate blood circulation, the energy of the dry stomach of invigorating the spleen, and there is hypotensive, reducing blood lipid, the effect such as hypoglycemic, antibacterial.
Bifidobacterium, formal name used at school: bifidobacterium, Bifidobacterium is the Gram-positive bacillus by French scholar Tissier isolated a kind of anaerobism from the ight soil of breast milk nutrition youngster in 1899, and end is bifurcated usually, therefore named Bifidobacterium.
Candida utili, formal name used at school: Candidautilis.Candida utili is again Torula utilis Henneb or edible torula.The content of its protein and Cobastab is all high than brewer's yeast, and it can using urea and nitric acid as nitrogenous source, and not needing to add any growth factor in the medium can grow.。
A kind of protecting liver and expelling toxin pectase provided by the invention, the main component of ferment, except multiple eating ferment, also has trace element, mineral matter, vitamin, amino acid, protein, carbohydrate etc.; Especially after employing enzyme live keeping agent, the activity of ferment is protected, and the outer ferment of various born of the same parents is complete, energetic.Ferment can for your individual physique, the absorption of adjustment health and metabolism, and is that you supplement the most omnibearing nutrition, can effective protecting liver and expelling toxin.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described further, the following stated, only to preferred embodiment of the present invention, not do other forms of restriction to the present invention, any those skilled in the art may utilize the technology contents of above-mentioned announcement to be changed to the Equivalent embodiments of equal change.Everyly do not depart from the present invention program's content, any simple modification done following examples according to technical spirit of the present invention or equivalent variations, all drop in protection scope of the present invention.
Embodiment 1
The preparation method of protecting liver and expelling toxin pectase, comprises the following steps:
(1) by red common beet 6kg, pineapple 5kg, carrot 1kg, apple 1kg, mulberry fruit 1kg, red date 1kg, agropyron 1kg water cleaning, be crushed to 3 orders, mix, obtain fruit and vegetable materials;
(2) in fruit and vegetable materials, add sugarcane raw sugar 4.5kg, chlorella 0.9kg, enzyme live keeping agent 0.13kg, probio 0.15kg, sealing, the temperature of fermentation is 35 DEG C, ferments 175 days, obtains tunning;
(3) used by tunning plate and frame filter to filter 2 times, adopt 100 object filter clothes, controlled pressure is 0.3Mpa, obtains filtrate;
(4) filtrate sealed, left at room temperature 115 days, obtains the ferment of embodiment 1.
Described enzyme live keeping agent is obtained for 1:1:1 is uniformly mixed in mass ratio by maltitol, hydroxyl isomaltulose, sweet mellow wine.
Described probio is obtained for 1:1:1:1 is uniformly mixed in mass ratio by monascus ruber, aspergillus oryzae, Bifidobacterium, candida utili.
Embodiment 2
According to the pulp furnish described in embodiment 1 and method preparation, difference is only: described probio is obtained for 1:1:1 is uniformly mixed in mass ratio by aspergillus oryzae, Bifidobacterium, candida utili.Obtain the protecting liver and expelling toxin pectase of embodiment 2.
Embodiment 3
According to the pulp furnish described in embodiment 1 and method preparation, difference is only: described probio is obtained for 1:1:1 is uniformly mixed in mass ratio by monascus ruber, Bifidobacterium, candida utili.Obtain the protecting liver and expelling toxin pectase of embodiment 3.
Embodiment 4
According to the pulp furnish described in embodiment 1 and method preparation, difference is only: described probio is obtained for 1:1:1 is uniformly mixed in mass ratio by monascus ruber, aspergillus oryzae, candida utili.Obtain the protecting liver and expelling toxin pectase of embodiment 4.
Embodiment 5
According to the pulp furnish described in embodiment 1 and method preparation, difference is only: described probio is obtained for 1:1:1 is uniformly mixed in mass ratio by monascus ruber, aspergillus oryzae, Bifidobacterium.Obtain the protecting liver and expelling toxin pectase of embodiment 5.
Embodiment 6
According to the pulp furnish described in embodiment 1 and method preparation, difference is only: described enzyme live keeping agent is obtained for 1:1 is uniformly mixed in mass ratio by hydroxyl isomaltulose, sweet mellow wine.Obtain the protecting liver and expelling toxin pectase of embodiment 6.
Embodiment 7
According to the pulp furnish described in embodiment 1 and method preparation, difference is only: described enzyme live keeping agent is obtained for 1:1 is uniformly mixed in mass ratio by maltitol, sweet mellow wine.Obtain the protecting liver and expelling toxin pectase of embodiment 7.
Embodiment 8
According to the pulp furnish described in embodiment 1 and method preparation, difference is only: described enzyme live keeping agent is obtained for 1:1 is uniformly mixed in mass ratio by maltitol, hydroxyl isomaltulose.Obtain the protecting liver and expelling toxin pectase of embodiment 8.
Test case 1
Adopt prolease activity in determined by ultraviolet spectrophotometry ferment, assay NBT photoreduction is adopted to measure the vigor of SOD in ferment, adopt iodine colorimetry to measure amylase activity with reference to " national clinical examination operational procedure ", with reference to QB/T1803-1993, adopt titration measuring lipase activity.In the protecting liver and expelling toxin pectase prepare embodiment 1-8, enzyme activity measures, and test result is in table 1:
Table 1 primary efficacy enzyme activity unit: U/mL
Comparing embodiment 1 and embodiment 2-5, embodiment 1 (monascus ruber, aspergillus oryzae, Bifidobacterium, candida utili composite) ferment enzyme vigor is apparently higher than embodiment 2-5 (in monascus ruber, aspergillus oryzae, Bifidobacterium, candida utili, any three is composite); Comparing embodiment 1 and embodiment 6-8, embodiment 1 (maltitol, hydroxyl isomaltulose, sweet mellow wine are composite) ferment enzyme vigor is apparently higher than embodiment 6-8 (in maltitol, hydroxyl isomaltulose, sweet mellow wine, the two is composite arbitrarily).
Test case 2
Liver effect measuring is protected to protecting liver and expelling toxin pectase prepared by embodiment 1-8.
Every group selection outpatient 20 example, wherein man 10 example, female 10 example, max age 60 years old, minimal ages 20 years old,
Clinical manifestation is alcoholic liver, moderate cirrhosis.
Experimental technique: take protecting liver and expelling toxin pectase every day 3 times, each 30mL, drinking with the water of 5 times 35 DEG C dilution, 12 weeks is a course for the treatment of.
The standard of curative effect evaluation:
Effective: to serve on this ferment after 1 month, transference cure, disease is healed on inspection.
Effective: to serve on this ferment after 1 month, symptom takes a turn for the better, and also has slight constipation symptom.
Invalid: serve on this ferment after 1 month, symptom is not improved or increased the weight of.
Collect data before and after patient therapy, carry out statistical analysis more afterwards, result shows P < 0.05, and difference has statistical significance.
Concrete test data is in table 3:
Table 3: constipation result for the treatment of comparison sheet
Experiment proves, the present invention effectively can protect liver, has good curative effect to hepatopathy.
Test case 3
Effect of ejecting poison mensuration is carried out to protecting liver and expelling toxin pectase prepared by embodiment 1-8.
Every group selection outpatient 20 example, wherein man 10 example, female 10 example, max age 60 years old, minimal ages 20 years old,
Clinical manifestation is constipation more than three months, below stool is secondary on every Wendesdays.
Experimental technique: take protecting liver and expelling toxin pectase every day 2 times, each 30mL, drinks with the water of 5 times 35 DEG C dilution, within 8 weeks, is a course for the treatment of, does not namely re-use after reaching normal defecation, follow up a case by regular visits to 4 weeks, see with or without rebound phenomenon.
The standard of curative effect evaluation:
Effective: to serve on this ferment after 1 month, constipation symptom disappears, and defecation is normal.
Effective: to serve on this ferment after 1 month, constipation symptom takes a turn for the better, and also has slight constipation symptom.
Invalid: serve on this ferment after 1 month, constipation symptom is not improved.
Collect data before and after patient therapy, carry out statistical analysis more afterwards, result shows P < 0.05, and difference has statistical significance.
Concrete test data is in table 3:
Table 3: constipation result for the treatment of comparison sheet
Experiment proves, the present invention effectively can improve constipation symptom, has good curative effect to constipation.
Claims (5)
1. the preparation method of protecting liver and expelling toxin pectase, is characterized in that, comprises the following steps:
(1) fruits and vegetables are pulverized;
(2) in the fruits and vegetables after pulverizing, sucrose, chlorella, enzyme live keeping agent, probio is added, sealing, fermentation;
(3) filter;
(4) sealing and standing.
Fruits and vegetables in described step (1), are made up of the raw material of following weight parts: red common beet 50-70 part, pineapple 40-60 part, carrot 5-15 part, apple 5-15 part, mulberry fruit 5-15 part, red date 5-15 part, agropyron 5-15 part.
In described step (2), sucrose addition is the 20-40wt% of fruits and vegetables gross weight; chlorella addition is the 2-12wt% of fruits and vegetables gross weight; enzyme live keeping agent addition is the 0.5-3.5wt% of fruits and vegetables gross weight, and probio addition is the 0.5-1.5wt% of fruits and vegetables gross weight.
Described enzyme live keeping agent is mixed by maltitol, hydroxyl isomaltulose, sweet mellow wine, and the mass ratio of described maltitol, hydroxyl isomaltulose, sweet mellow wine is (1-3): (1-3): (1-3).
Described probio is mixed by monascus ruber, aspergillus oryzae, Bifidobacterium, candida utili, and the mass ratio of described monascus ruber, aspergillus oryzae, Bifidobacterium, candida utili is (1-3): (1-3): (1-3): (1-3).
2. the preparation method of protecting liver and expelling toxin pectase as claimed in claim 1, is characterized in that, in described step (2), the temperature of fermentation is 35-41 DEG C, and the time is 140-210 days.
3. the preparation method of protecting liver and expelling toxin pectase as claimed in claim 1, is characterized in that, described step (3) is filtered and used plate and frame filter to filter 1-3 time, and adopt 60-120 object filter cloth, controlled pressure is 0.1-0.5Mpa.
4. the preparation method of protecting liver and expelling toxin pectase as claimed in claim 1, it is characterized in that, described step (4) the sealing and standing time is 85-145 days.
5. a protecting liver and expelling toxin pectase, is characterized in that, adopts method according to any one of claim 1-4 to be prepared from.
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Application publication date: 20160511 |