CN107439875A - A kind of Tea Caffeine green turnip ferment Traditional Chinese medicine potion and preparation method thereof - Google Patents
A kind of Tea Caffeine green turnip ferment Traditional Chinese medicine potion and preparation method thereof Download PDFInfo
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- 235000011293 Brassica napus Nutrition 0.000 title claims abstract description 127
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- LPHGQDQBBGAPDZ-UHFFFAOYSA-N Isocaffeine Natural products CN1C(=O)N(C)C(=O)C2=C1N(C)C=N2 LPHGQDQBBGAPDZ-UHFFFAOYSA-N 0.000 title claims abstract description 115
- 229960001948 caffeine Drugs 0.000 title claims abstract description 115
- VJEONQKOZGKCAK-UHFFFAOYSA-N caffeine Natural products CN1C(=O)N(C)C(=O)C2=C1C=CN2C VJEONQKOZGKCAK-UHFFFAOYSA-N 0.000 title claims abstract description 115
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- 238000002360 preparation method Methods 0.000 title claims description 13
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
- A23L2/382—Other non-alcoholic beverages fermented
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a kind of Tea Caffeine green turnip ferment Traditional Chinese medicine potion, the Tea Caffeine green turnip ferment Traditional Chinese medicine potion is prepared by the fermentation of tea leaching liquor, black tea coffee alkali extracting solution, green turnip, chlorella algae powder, zymophyte and sucrose, the Tea Caffeine green turnip ferment Traditional Chinese medicine potion of the present invention can improve the digestibility and absorbing proteins efficiency of chlorella, promote gastrointestinal peristalsis, reduce blood fat, softening blood vessel and stabilizing blood pressure；Black tea coffee alkali extracting solution promotes insulin secretion and reduces type II diabetes risk with that can improve blood circulation after other raw materials synergy in Tea Caffeine green turnip ferment Traditional Chinese medicine potion.In addition, Tea Caffeine green turnip ferment Traditional Chinese medicine potion excellent taste of the invention, its color and luster is light reddish brown color, and uniformity, juice turbidity is uniform, and free from admixture, delicate mouthfeel is soft, and sugariness is moderate, and smell is fresh and sweet, there is the delicate fragrance of black tea and green turnip.
Description
Technical field
The invention belongs to drug preparation technique field, relates in particular to a kind of Tea Caffeine green turnip ferment Chinese medicine drink
Agent and preparation method thereof.
Background technology
Various trace elements and bioactive substance are rich in microalgae cell, as polysaccharide, protein, unrighted acid,
Pigment etc., it is not only turned into indispensable important bait in aquatic economic animals seedling raising process, but also turn into mankind pole
Good natural health care.In addition, microalgae is in food, medicine, feed addictive, genetic engineering, cosmetics, chemical industry, environmental protection etc.
Field also all has broad application prospects.Wherein, chlorella is universally acknowledged novel health food, contains abundant albumen
Contain a kind of chlorella growth in the nutriments such as matter, nucleic acid, polysaccharide, vitamin, lipid, trace element, particularly chlorella
The factor, it can promote immunity of organism, and reinforced immunological function, thus enjoy the praise of various countries' scientific research scholar and nutritionist and
Praise highly.
At present, chlorella is to have realized one of microalgae species of industrialization production, because chlorella is unicellular alga, carefully
Cell wall is thick and structure is special, and the digestibility and absorbing proteins of the directly edible chlorella of people are less efficient, thus edible chlorella is led to
Often need to carry out deep processing processing.But the technology also very shortcoming of Traditional Chinese medicine potion so far, is made, it is necessary to science and technology using chlorella
The concern and research of personnel.It is contemplated that chlorella future has extensively in the related industries such as food, beverage, medicine, health products
Wealthy application prospect.
The content of the invention
In view of the shortcomings of the prior art, it is an object of the invention to provide a kind of Tea Caffeine green turnip ferment Traditional Chinese medicine potion
And preparation method thereof.
The purpose of the present invention is achieved by following technical proposals.
A kind of Tea Caffeine green turnip ferment Traditional Chinese medicine potion, the Tea Caffeine green turnip ferment Traditional Chinese medicine potion are soaked by tea
Extract, black tea coffee alkali extracting solution, green turnip, chlorella algae powder, zymophyte and sucrose fermentation are prepared.
The preparation method of above-mentioned Tea Caffeine green turnip ferment Traditional Chinese medicine potion, comprises the following steps：
Step 1, low caffeine black tea is put into water-bath in 70~90 DEG C of water and extracts 10~60min, filtering, obtain tea leaching
Extract, wherein, in parts by mass, the ratio of the low caffeine black tea and water is 1：(45~55)；
In step 1, the content of caffeine is 27~33mg/g, preferably 27~32mg/ in the low caffeine black tea
g。
In step 1, low caffeine black tea is put into water-bath in 75~85 DEG C of water and extracts 15~40min, filtering, obtained
Tea leaching liquor.
Step 2, high caffeine black tea is crushed, crosses 16~50 mesh sieves, the high caffeine is extracted with circumfluence distillation device
The caffeine of black tea, black tea coffee alkali extracting solution is obtained, it is standby；Wherein, extracting parameter is：In parts by mass, the high coffee
The ratio of alkali black tea and water is 1：(10~20), return time are 30~90min；
In step 2, the content of caffeine is 33~46mg/g, preferably 43~46mg/ in the high caffeine black tea
g。
Step 3, green turnip is put into 80~100 DEG C of water and boils 5~15min, pull green turnip out, be cooled to room temperature 20~25
DEG C, the green turnip is added in the tea leaching liquor, is mixed with beating, obtains slurry；Wherein, in parts by mass, the green grass or young crops
The ratio of radish and tea leaching liquor is (1~3)：(11~13)；
In step 3, in parts by mass, the ratio of green turnip and the tea leaching liquor is (7~12)：(57~61).
Step 4, chlorella algae powder, zymophyte and sucrose are added in the slurry, stirring makes it well mixed, uniformly mixed
Fermented 5~8 days at 22~32 DEG C after conjunction, the slurry after fermentation is filtered, fermented liquid is obtained after filtering；Wherein, wherein,
The zymophyte is bread microzyme (Saccharomyces cerevisiae), ferment bacillus (Bacillus
Subtilis) or the Mixed Microbes of the bread microzyme and ferment bacillus, bread microzyme are added in the form of dry bacterium powder
Add, ferment bacillus is added in the form of the nutrient solution of ferment bacillus, the ferment in the nutrient solution of ferment bacillus
The concentration of plain bacillus is 1.2 × 109CFU/mL；Mixed Microbes are added in the form of mixed bacteria liquid, in mixed bacteria liquid, face
The mass fraction of bag saccharomycete is consistent with the volume parts of the nutrient solution of ferment bacillus, is 1g per a mass fraction, often
A volume parts are 1mL；In parts by mass, the ratio of the slurry, chlorella algae powder and sucrose is 100：(3~7)：(2~
4)；
Wherein, based on parts by volume, the ratio of the slurry and the nutrient solution of the ferment bacillus is 100：(0.7~
1.2),
In parts by mass, the ratio of the slurry and bread microzyme is 100：(0.7~1.2)；
Based on parts by volume, the ratio of the slurry and mixed bacteria liquid is 100：(0.7~1.2)；
In step 4, chlorella algae powder, zymophyte and sucrose are added in 20~32 DEG C of the slurry.
In step 4, the sieve mesh number that the slurry is filtered is 16~100 mesh.
In step 4, the ferment bacillus is bacillus subtilis (Bacillus through strain idenfication
Subtilis it is general), and by bacterial strain Bacillus subtilis Liu-3 to be stored in China Committee for Culture Collection of Microorganisms
Logical microorganism center, culture presevation day：2013-08-15, culture presevation number are：CGMCC No.8026.
Step 5, the black tea coffee alkali extracting solution described in step 2, mixing are added in described fermented liquid in step 4
To uniform, Tea Caffeine green turnip ferment Traditional Chinese medicine potion is obtained；Wherein, in parts by mass, the black tea coffee alkali extracting solution and
The ratio of green turnip described in step 3 is (1~3)：(1~3).
In the above-mentioned technical solutions, after the step 5, the Tea Caffeine green turnip ferment Traditional Chinese medicine potion is entered
Row sterilizing：In 100 DEG C of 8~15min of water-bath, 20~25 DEG C of room temperature is naturally cooled to.
Compared to prior art, Tea Caffeine green turnip ferment Traditional Chinese medicine potion of the invention can improve disappearing for chlorella
Rate and absorbing proteins efficiency, promote gastrointestinal peristalsis, reduce blood fat, softening blood vessel and stabilizing blood pressure；Black tea coffee alkali extracting solution
With blood circulation can be improved after other raw materials synergy in Tea Caffeine green turnip ferment Traditional Chinese medicine potion, promote pancreas islet
Element secretion and reduction type II diabetes risk.In addition, Tea Caffeine green turnip ferment Traditional Chinese medicine potion mouthfeel pole of the invention
Good, its color and luster is light reddish brown color, and uniformity, juice turbidity is uniform, and free from admixture, delicate mouthfeel is soft, and sugariness is moderate, smell
Sweetness, there is the delicate fragrance of black tea and green turnip.
Embodiment
In the present invention, coffee in black tea is extracted with circumfluence distillation device (apparatus,Soxhlet's of circumfluence distillation device)
The method of alkali is：Zeolite (adsorbing contaminant) is put into round-bottomed flask, padding an absorbent cotton in constant pressure funnel bottom (prevents
Leaked under black tea tea dust), weigh black tea and obtain black tea quality, black tea is crushed and crosses 30 mesh sieves, constant pressure addition leakage is put into after sieving
In bucket, water is injected into constant pressure funnel, after soaking tea dust 15min, is flowed liquid into round-bottomed flask, into condenser
Cooling water is passed through, electric jacket heating round-bottomed flask, after solution boiling (100 DEG C) in round-bottomed flask, adjusts constant pressure funnel
Piston, make condensate liquid instillation speed be equal to constant pressure funnel leakage speed, 30~90min of refluxing extraction, obtain red
Tea coffee alkali extracting solution.
Reagent used in the content of caffeine includes in measure black tea (black tea coffee alkali extracting solution)：Caffeine standard items
(domestic analysis is pure, is purchased from Tianjin Tai Airui Science and Technology Ltd.s)；(domestic analysis is pure, and it is close to be purchased from Tianjin section for basic lead acetate
Europe chemical reagent Co., Ltd)；Hydrochloric acid, sulfuric acid etc. are that domestic analysis is pure.
The specific method of caffeine content is in measure black tea (black tea coffee alkali extracting solution)：Take 10mL black tea coffee alkali carries
Liquid is taken to add 4mL0.01mol/L hydrochloric acid and 1mL basic lead acetates solution (basic lead acetate solution concentration to 100mL volumetric flasks
For 0.5g/mL), scale (100mL) is settled to deionized water, is mixed, stands, clarification filtration, obtains filtrate.Take 25mL filtrates
Into 50mL volumetric flasks, 0.1mL 4.5mol/L sulfuric acid solution is added, is diluted with water to scale (50mL), mixed, stood clear
Clear filtering, with 10mm quartz cuvettes, under wavelength 274nm, reference, measure absorbance (A) are made with blank reagent solution.
Standard curve is made with caffeine standard items (purity is more than 99%).Reference is made with blank reagent solution, measure is inhaled
Luminosity (A).Using absorbance OD values as ordinate y, black tea coffee alkali concn is abscissa x, draws standard curve.Standard curve returns
It is y=0.0430x-0.0103, R to return equation2=0.9993, therefore this standard curve can use.
With caffeine content divided by black tea quality in black tea coffee alkali extracting solution, caffeine content in every gram of black tea is obtained.
Black tea kind：Anhua black tea, it is purchased from yiyang, hunan lucky chance small stream tea house；Fengqing Yunnan black tea tea, it is purchased from Yunnan Yunnan black tea group
Limited company；Jin Junmei, it is purchased from Wuyi Mountain Ming Cong Tea Industry Co., Ltd；Sunshine black tea, it is purchased from Rizhao City north pile spring tea industry
There is Co., Ltd；Keemun black tea, it is purchased from Anhui osmanthus son's high mountain tea shop；River black tea, is purchased from Sichuan Linhu Tea Industry Co., Ltd.；English
Moral black tea, it is purchased from Guangdong Qingyuan mountain leaf scented tea agriculture cooperative society.Above black tea sample number consecutively be I, II, III, IV, V, VI,
Ⅶ.The caffeine that method according to said extracted black tea caffeine extracts above-mentioned multiple kind black tea (extracts black tea caffeine
Design parameter is：Return time is 60min, is flowed back 1 time；In parts by mass, the ratio of black tea and water is 1：10.), respectively obtain 7
Kind black tea coffee alkali extracting solution, by the way that the content of caffeine in different black tea is calculated, the results are shown in Table 1.
The content of caffeine in the different cultivars black tea of table 1
Therefore, in an embodiment of the present invention, low caffeine black tea uses coffee in black tea (black tea coffee alkali extracting solution)
The relatively low Yingteh graded black tea of alkali content, high caffeine black tea use caffeine content in black tea (black tea coffee alkali extracting solution) higher
Anhua black tea.
Used green turnip is block (radish peeling) in an embodiment of the present invention, and size is 2~5cm.Through extensive
Frustule is cultivated, frustule (precipitation method, centrifugal process etc. can be used), then broken wall is then collected, is dried to obtain chlorella algae
Powder, chlorella algae powder purchase in the embodiment of the present invention is from Wudi Lv Qi bioengineering Co., Ltd, 120 mesh (chlorella algae powders
Granularity size), broken wall type；Bread microzyme is bought from Angel Yeast Co., Ltd；During ferment bacillus is derived from
State's Microbiological Culture Collection administration committee common micro-organisms center, culture presevation number are：CGMCC No.8026.Ferment gemma
The concentration of ferment bacillus is 1.2 × 10 in the nutrient solution of bacillus9CFU/mL；In mixed bacteria liquid, the matter of bread microzyme
It is consistent with the volume parts of the nutrient solution of ferment bacillus to measure number, is 1g per a mass fraction, per a volume parts
For 1mL；Wherein, the fluid nutrient medium for cultivating ferment bacillus is LB culture mediums, and its formula is：Tryptone (Tryptone)
10g/L；Yeast extract (Yeast extract) 5g/L；Sodium chloride (NaCl) 10g/L, the LB culture mediums are adjusted with NaOH
PH, reach 7.4.
Ferment one day is 24 hours.
The Tea Caffeine green turnip ferment Traditional Chinese medicine potion of the present invention is described in detail with reference to embodiment.
Embodiment 1
Step 1, Yingteh graded black tea (i.e. low caffeine black tea) is put into water-bath in 80 DEG C of water and extracts 15min, filtering, obtained
Tea leaching liquor, wherein, in parts by mass, the ratio of the Yingteh graded black tea and water is 1：50；
Step 2, crush and pacify magnificent black tea (i.e. high caffeine black tea), cross 30 mesh sieves, use circumfluence distillation according to the method described above
The caffeine of magnificent black tea is pacified in device extraction, obtains black tea coffee alkali extracting solution, standby.Wherein, extracting parameter is：By mass parts
Meter, the ratio for pacifying magnificent black tea and water is 1：10, return time 60min；
Step 3, the green turnip of bulk is put into 100 DEG C of water and boils 10min, pulled green turnip out, be cooled to room temperature 20~25
DEG C, green turnip is added in 20~25 DEG C of tea leaching liquor, is mixed with beating, obtains slurry；Wherein, in parts by mass, it is described
The ratio of green turnip and tea leaching liquor is 1：11；
Step 4, chlorella algae powder, bread microzyme and sucrose are added in 20~25 DEG C of slurry, stirring makes its mixing
Uniformly, uniformly fermented 7 days at 27 DEG C after mixing；Wherein, in parts by mass, the slurry, chlorella algae powder, bread microzyme and
The ratio of sucrose is 100：5：1：2；Slurry after fermentation is filtered and (crosses 50 mesh sieves), fermented liquid is obtained after filtering；
Step 5, the black tea coffee alkali extracting solution added in fermented liquid in step 4 in step 2, mix to uniform,
Obtain Tea Caffeine green turnip ferment Traditional Chinese medicine potion；Wherein, in parts by mass, described in black tea coffee alkali extracting solution and step 3
The ratio of green turnip is 1:1.
Embodiment 2
Step 1, Yingteh graded black tea is put into water-bath in 80 DEG C of water and extracts 15min, filtering, obtain tea leaching liquor, wherein, press
Mass parts meter, the ratio of the Yingteh graded black tea and water is 1：50；
Step 2, crush and pacify magnificent black tea, cross 30 mesh sieves, pacify magnificent black tea with the extraction of circumfluence distillation device according to the method described above
Caffeine, obtain black tea coffee alkali extracting solution, it is standby.Wherein, extracting parameter is：In parts by mass, the magnificent black tea of the peace and
The ratio of water is 1：10, return time 60min；
Step 3, the green turnip of bulk is put into 100 DEG C of water and boils 10min, pulled green turnip out, be cooled to room temperature 20~25
DEG C, green turnip is added in tea leaching liquor, is mixed with beating, obtains slurry；Wherein, in parts by mass, the green turnip and tea
The ratio of leaching liquor is 1：6；
Step 4, chlorella algae powder, bread microzyme and sucrose are added in the slurry, and stirring makes it well mixed, uniformly mixed
Fermented 7 days at 28 DEG C after conjunction；Wherein, in parts by mass, the ratio of the slurry, chlorella algae powder, bread microzyme and sucrose is
100：5：1：3；Slurry after fermentation is filtered and (crosses 50 mesh sieves), fermented liquid is obtained after filtering；Step 5, in step 4
Fermented liquid in add the black tea coffee alkali extracting solution in step 2, mix to uniform, obtain Tea Caffeine green turnip ferment
Traditional Chinese medicine potion；Wherein, in parts by mass, the ratio of green turnip described in black tea coffee alkali extracting solution and step 3 is 1：2.
Embodiment 3
Step 1, Yingteh graded black tea is put into water-bath in 80 DEG C of water and extracts 15min, filtering, obtain tea leaching liquor, wherein, press
Mass parts meter, the ratio of the Yingteh graded black tea and water is 1：50；
Step 2, crush and pacify magnificent black tea, cross 30 mesh sieves, pacify magnificent black tea with the extraction of circumfluence distillation device according to the method described above
Caffeine, obtain black tea coffee alkali extracting solution, it is standby.Wherein, extracting parameter is：In parts by mass, the magnificent black tea of the peace and
The ratio of water is 1：10, return time 60min；
Step 3, the green turnip of bulk is put into 100 DEG C of water and boils 10min, pulled green turnip out, be cooled to room temperature 20~25
DEG C, green turnip is added in tea leaching liquor, is mixed with beating, obtains slurry；Wherein, in parts by mass, the green turnip and tea
The ratio of leaching liquor is 3：13；
Step 4, chlorella algae powder, bread microzyme and sucrose are added in the slurry, and stirring makes it well mixed, uniformly mixed
Fermented 7 days at 29 DEG C after conjunction；Wherein, in parts by mass, the ratio of the slurry, chlorella algae powder, bread microzyme and sucrose is
100：5：1：4；Slurry after fermentation is filtered and (crosses 50 mesh sieves), fermented liquid is obtained after filtering；Step 5, in step 4
Fermented liquid in add the black tea coffee alkali extracting solution in step 2, mix to uniform, obtain Tea Caffeine green turnip ferment
Traditional Chinese medicine potion；Wherein, in parts by mass, the ratio of green turnip described in black tea coffee alkali extracting solution and step 3 is 1：3.
Embodiment 4
Step 1, Yingteh graded black tea is put into water-bath in 80 DEG C of water and extracts 15min, filtering, obtain tea leaching liquor, wherein, press
Mass parts meter, the ratio of the Yingteh graded black tea and water is 1：50；
Step 2, crush and pacify magnificent black tea, cross 30 mesh sieves, pacify magnificent black tea with the extraction of circumfluence distillation device according to the method described above
Caffeine, obtain black tea coffee alkali extracting solution, it is standby.Wherein, extracting parameter is：In parts by mass, the magnificent black tea of the peace and
The ratio of water is 1：10, return time 60min；
Step 3, the green turnip of bulk is put into 100 DEG C of water and boils 10min, pulled green turnip out, be cooled to room temperature 20~25
DEG C, green turnip is added in tea leaching liquor, is mixed with beating, obtains slurry；Wherein, in parts by mass, the green turnip and tea
The ratio of leaching liquor is 1：12；
Step 4, chlorella algae powder, bread microzyme and sucrose are added in the slurry, and stirring makes it well mixed, uniformly mixed
Fermented 7 days at 30 DEG C after conjunction；Wherein, in parts by mass, the ratio of the slurry, chlorella algae powder, bread microzyme and sucrose is
100：5：1：4；Slurry after fermentation is filtered and (crosses 50 mesh sieves), fermented liquid is obtained after filtering；Step 5, in step 4
Fermented liquid in add the black tea coffee alkali extracting solution in step 2, mix to uniform, obtain Tea Caffeine green turnip ferment
Traditional Chinese medicine potion；Wherein, in parts by mass, the ratio of green turnip described in black tea coffee alkali extracting solution and step 3 is 2：1.
Embodiment 5
Step 1, Yingteh graded black tea is put into water-bath in 80 DEG C of water and extracts 15min, filtering, obtain tea leaching liquor, wherein, press
Mass parts meter, the ratio of the Yingteh graded black tea and water is 1：50；
Step 2, crush and pacify magnificent black tea, cross 30 mesh sieves, pacify magnificent black tea with the extraction of circumfluence distillation device according to the method described above
Caffeine, obtain black tea coffee alkali extracting solution, it is standby.Wherein, extracting parameter is：In parts by mass, the magnificent black tea of the peace and
The ratio of water is 1：10, return time 60min；
Step 3, the green turnip of bulk is put into 100 DEG C of water and boils 10min, pulled green turnip out, be cooled to room temperature 20~25
DEG C, green turnip is added in tea leaching liquor, is mixed with beating, obtains slurry；Wherein, in parts by mass, the green turnip and tea
The ratio of leaching liquor is 2：11；
Step 4, chlorella algae powder, bread microzyme and sucrose are added in the slurry, and stirring makes it well mixed, uniformly mixed
Fermented 7 days at 23 DEG C after conjunction；Wherein, in parts by mass, the ratio of the slurry, chlorella algae powder, bread microzyme and sucrose is
100：5：1：2；Slurry after fermentation is filtered and (crosses 50 mesh sieves), fermented liquid is obtained after filtering；Step 5, in step 4
Fermented liquid in add the black tea coffee alkali extracting solution in step 2, mix to uniform, obtain Tea Caffeine green turnip ferment
Traditional Chinese medicine potion；Wherein, in parts by mass, the ratio of green turnip described in black tea coffee alkali extracting solution and step 3 is 1：1.
Embodiment 6
Step 1, Yingteh graded black tea is put into water-bath in 80 DEG C of water and extracts 15min, filtering, obtain tea leaching liquor, wherein, press
Mass parts meter, the ratio of the Yingteh graded black tea and water is 1：50；
Step 2, crush and pacify magnificent black tea, cross 30 mesh sieves, pacify magnificent black tea with the extraction of circumfluence distillation device according to the method described above
Caffeine, obtain black tea coffee alkali extracting solution, it is standby.Wherein, extracting parameter is：In parts by mass, the magnificent black tea of the peace and
The ratio of water is 1：10, return time 60min；
Step 3, the green turnip of bulk is put into 100 DEG C of water and boils 10min, pulled green turnip out, be cooled to room temperature 20~25
DEG C, green turnip is added in tea leaching liquor, is mixed with beating, obtains slurry；Wherein, in parts by mass, the green turnip and tea
The ratio of leaching liquor is 3：11；
Step 4, chlorella algae powder, the nutrient solution and sucrose of ferment bacillus are added in the slurry, and stirring makes its mixing
Uniformly, uniformly fermented 7 days at 30 DEG C after mixing；Wherein, in parts by mass, the ratio of the slurry, chlorella algae powder and sucrose is
100：5：3, based on parts by volume, the ratio of the nutrient solution of slurry and the ferment bacillus is 100：1；Slurry after fermentation is entered
Row filtering (crosses 50 mesh sieves), and fermented liquid is obtained after filtering；
Step 5, the black tea coffee alkali extracting solution added in fermented liquid in step 4 in step 2, mix to uniform,
Obtain Tea Caffeine green turnip ferment Traditional Chinese medicine potion；Wherein, in parts by mass, described in black tea coffee alkali extracting solution and step 3
The ratio of green turnip is 2：3.
Embodiment 7
Step 1, Yingteh graded black tea is put into water-bath in 80 DEG C of water and extracts 15min, filtering, obtain tea leaching liquor, wherein, press
Mass parts meter, the ratio of the Yingteh graded black tea and water is 1：50；
Step 2, crush and pacify magnificent black tea, cross 30 mesh sieves, pacify magnificent black tea with the extraction of circumfluence distillation device according to the method described above
Caffeine, obtain black tea coffee alkali extracting solution, it is standby.Wherein, extracting parameter is：In parts by mass, the magnificent black tea of the peace and
The ratio of water is 1：10, return time 60min；
Step 3, the green turnip of bulk is put into 100 DEG C of water and boils 10min, pulled green turnip out, be cooled to room temperature 20~25
DEG C, green turnip is added in tea leaching liquor, is mixed with beating, obtains slurry；Wherein, in parts by mass, the green turnip and tea
The ratio of leaching liquor is 1：13；
Step 4, chlorella algae powder, the nutrient solution and sucrose of ferment bacillus are added in the slurry, and stirring makes its mixing
Uniformly, uniformly fermented 7 days at 23 DEG C after mixing；Wherein, in parts by mass, the ratio of the slurry, chlorella algae powder and sucrose is
100：5：3, based on parts by volume, the ratio of the nutrient solution of slurry and the ferment bacillus is 100：1；Slurry after fermentation is entered
Row filtering (crosses 50 mesh sieves), and fermented liquid is obtained after filtering；
Step 5, the black tea coffee alkali extracting solution added in fermented liquid in step 4 in step 2, mix to uniform,
Obtain Tea Caffeine green turnip ferment Traditional Chinese medicine potion；Wherein, in parts by mass, described in black tea coffee alkali extracting solution and step 3
The ratio of green turnip is 3：1.
Embodiment 8
Step 1, Yingteh graded black tea is put into water-bath in 80 DEG C of water and extracts 15min, filtering, obtain tea leaching liquor, wherein, press
Mass parts meter, the ratio of the Yingteh graded black tea and water is 1：50；
Step 2, crush and pacify magnificent black tea, cross 30 mesh sieves, pacify magnificent black tea with the extraction of circumfluence distillation device according to the method described above
Caffeine, obtain black tea coffee alkali extracting solution, it is standby.Wherein, extracting parameter is：In parts by mass, the magnificent black tea of the peace and
The ratio of water is 1：10, return time 60min；
Step 3, the green turnip of bulk is put into 100 DEG C of water and boils 10min, pulled green turnip out, be cooled to room temperature 20~25
DEG C, green turnip is added in tea leaching liquor, is mixed with beating, obtains slurry；Wherein, in parts by mass, the green turnip and tea
The ratio of leaching liquor is 2：11；
Step 4, chlorella algae powder, the nutrient solution and sucrose of ferment bacillus are added in the slurry, and stirring makes its mixing
Uniformly, uniformly fermented 7 days at 25 DEG C after mixing；Wherein, in parts by mass, the ratio of the slurry, chlorella algae powder and sucrose is
100：5：4, based on parts by volume, the ratio of the nutrient solution of slurry and the ferment bacillus is 100：1；Slurry after fermentation is entered
Row filtering (crosses 50 mesh sieves), and fermented liquid is obtained after filtering；
Step 5, the black tea coffee alkali extracting solution added in fermented liquid in step 4 in step 2, mix to uniform,
Obtain Tea Caffeine green turnip ferment Traditional Chinese medicine potion；Wherein, in parts by mass, described in black tea coffee alkali extracting solution and step 3
The ratio of green turnip is 3：2.
Embodiment 9
Step 1, Yingteh graded black tea is put into water-bath in 80 DEG C of water and extracts 15min, filtering, obtain tea leaching liquor, wherein, press
Mass parts meter, the ratio of the Yingteh graded black tea and water is 1：50；
Step 2, crush and pacify magnificent black tea, cross 30 mesh sieves, pacify magnificent black tea with the extraction of circumfluence distillation device according to the method described above
Caffeine, obtain black tea coffee alkali extracting solution, it is standby.Wherein, extracting parameter is：In parts by mass, the magnificent black tea of the peace and
The ratio of water is 1：10, return time 60min；
Step 3, the green turnip of bulk is put into 100 DEG C of water and boils 10min, pulled green turnip out, be cooled to room temperature 20~25
DEG C, green turnip is added in tea leaching liquor, is mixed with beating, obtains slurry；Wherein, in parts by mass, the green turnip and tea
The ratio of leaching liquor is 3：12；
Step 4, chlorella algae powder, mixed bacteria liquid and sucrose are added in the slurry, and stirring makes it well mixed, uniformly mixing
Fermented 7 days at 25 DEG C afterwards；Wherein, in parts by mass, the ratio of the slurry, chlorella algae powder and sucrose is 100：5：2, by volume
Part meter, the ratio of the slurry and mixed bacteria liquid is 100：1；Slurry after fermentation is filtered and (crosses 50 mesh sieves), after filtering
To fermented liquid；
Step 5, the black tea coffee alkali extracting solution added in fermented liquid in step 4 in step 2, mix to uniform,
Obtain Tea Caffeine green turnip ferment Traditional Chinese medicine potion；Wherein, in parts by mass, described in black tea coffee alkali extracting solution and step 3
The ratio of green turnip is 1：1.
In above-described embodiment 1~9, the Tea Caffeine green turnip ferment Traditional Chinese medicine potion obtained by step 5 is sterilized：
In the vial that the Tea Caffeine green turnip ferment Traditional Chinese medicine potion prepared loading cleaning and sterilizing is crossed, by bottled tealeaves coffee
Alkali green turnip ferment Traditional Chinese medicine potion, which is put into 100 DEG C of boiling water, keeps 10min, is cooled to 20~25 DEG C of room temperature.
Appreciation evaluation by color, smell and taste, when extraction temperature is 80 DEG C, Tea Caffeine green turnip ferment Chinese medicine of the present invention
Potus has optimal mouthfeel.When the ratio of Yingteh graded black tea and water is 1：When 50, the effect of tea leaching liquor is best, bright, flavour
Mellow, quality is splendid.
Contain chlorella algae powder, chlorella (Chlorella in the Tea Caffeine green turnip ferment Traditional Chinese medicine potion of the present invention
Pyrenoidesa it is) nutritious, there is plurality of health care functions, protein content is close to 60g, folic acid in 100g chlorella algae powders
Content 1.2mg, chlorophyll content 7000mg, biological 0.18~0.25mg of cellulose content, content of cellulose 3.1g, active growth factor
(CGF) content 5000mg, polysaccharides content 8.8g, calcium content 230mg, iron content 86mg, Se content 30 μ g, Zn content 4mg, magnesium
Content 243mg, potassium content 1200mg, carotene carotene content 26mg, vitamin B1, B2, B6, C, D, E content about 30~90mg.
Wherein, as added 5g chlorella algae powders in 100g slurry, then increased each nutrition content is：Protein content approaches
3g, folate content 0.06mg, chlorophyll content 350mg, biological 0.009~0.0125mg of cellulose content, content of cellulose 0.155g,
Active growth factor (CGF) content 250mg, polysaccharides content 0.44g, calcium content 11.5mg, iron content 4.3mg, Se content 1.5
μ g, Zn content 0.2mg, content of magnesium 12.15mg, potassium content 60mg, carotene carotene content 1.3mg, vitamin B1、B2、B6、C、D、E
Content about 1.5~4.5mg.It can be obtained by experiment, compared to chlorella algae powder is directly taken, drink the tealeaves of the present invention
After caffeine green turnip ferment Traditional Chinese medicine potion, by the synergy of each raw material, the digestibility of chlorella algae powder can improve
15%, absorbing proteins efficiency improves 9%.
The present invention is fermented by tea leaching liquor, black tea coffee alkali extracting solution, green turnip, chlorella algae powder, zymophyte and sucrose to be made
It is standby to form, have the function that to reduce blood fat, stabilizing blood pressure and promote gastrointestinal peristalsis.Above-mentioned each raw material produces collaboration and made each other
With making it produce the therapeutic effect jointly.
Caffeine in tealeaves (black tea) is influenceed by local tea variety, manufacture craft, the place of production and extracting method, and caffeine contains
Amount is uncertain, chooses suitable tealeaves and extraction process can determine that the content of caffeine in tealeaves.Caffeine have a variety of physiology and
The effect of health care, it is possible to increase memory and recognition capability, alleviate the weak caused human-body fatigue of nervous system.In the present invention,
Black tea coffee alkali extracting solution is flat with that can be relaxed after other raw materials synergy in Tea Caffeine green turnip ferment Traditional Chinese medicine potion
Sliding flesh, increases painstaking effort output quantity, improves blood circulation, accelerates heartbeat；Promote insulin secretion and reduce type II diabetes risk；
With powerful diuresis, promote the excretion of urine；There is anti-oxidant, anticancer to become, be anti-for appropriate black tea coffee alkali extracting solution
Allergy, eliminate the multi-effects such as hydroxyl radical free radical, anti-inflammatory.Caffeine can rouse oneself nerve, refresh the mind.Tea is mutually tied with green turnip
Close, aromatic time of the crisp and refreshing combination black tea of green turnip is sweet, brings different mouth feeling experience.
200 people (man, each 100 people of female, including 100 a middle-aged persons, 100 the elderlys.Wherein, 100 are that hypertension is suffered from
Person, 100 are hyperlipemic patients, and have 20 defecations to have some setbacks in hyperpietic and hyperlipemic patients) drink above-mentioned implementation
The Tea Caffeine green turnip ferment Traditional Chinese medicine potion of example 1~9, drinking method (it is applied) are：Take 15 days, 9 points of morning and evening
, respectively drink 100ml at 8 points.The hypertension symptom of 96 hyperpietics is alleviated, and the hyperlipidemic conditions of 97 hyperlipemic patients delay
Solution, the defecation patient that has some setbacks start to become regular.
The present invention Tea Caffeine green turnip ferment Traditional Chinese medicine potion not only there is therapeutic effect, and drink it is good in taste,
Black tea is combined with green turnip juice, and black tea collides with the crisp and refreshing of green turnip juice, and the raw material of each component cooperates with caused mouthfeel each other
Fine and smooth soft, sugariness is moderate, and smell is fresh and sweet, there is the delicate fragrance of black tea and green turnip.It is evaluation group to select 15 professionals, is adopted
With hundred-mark system methods of marking, sense organ appraise is carried out to the color and luster of each drink, structural state, mouthfeel, smell, evaluation table is shown in Table 2, commented
Minute mark standard is shown in Table 3.
The Tea Caffeine green turnip ferment Traditional Chinese medicine potion embodiment evaluation table of table 2
| Embodiment | Color and luster | Structural state | Mouthfeel | Smell | Total score |
| Embodiment 1 | 16 | 17 | 30.43 | 16 | 79.43 |
| Embodiment 2 | 18 | 17.5 | 37.29 | 17.5 | 90.29 |
| Embodiment 3 | 17 | 16.7 | 33.16 | 16 | 82.86 |
| Embodiment 4 | 16.5 | 16.2 | 27.87 | 16 | 76.57 |
| Embodiment 5 | 16 | 15 | 26 | 15 | 72.00 |
| Embodiment 6 | 16 | 15.5 | 27.07 | 16 | 74.57 |
| Embodiment 7 | 15 | 15 | 26.57 | 15 | 71.57 |
| Embodiment 8 | 14 | 14 | 25.86 | 15 | 68.86 |
| Embodiment 9 | 14 | 14 | 22.93 | 15.5 | 66.43 |
The teabag drink sensory evaluation scores standard of table 3
As shown in Table 2, in embodiment 1~9, Tea Caffeine green turnip ferment Traditional Chinese medicine potion prepared by embodiment 2
Sensory evaluation scores highest, overall merit are best.
The content recorded according to present invention part carries out technical arrangement plan and the selection of different black tea can be achieved
The preparation of Tea Caffeine green turnip ferment Traditional Chinese medicine potion, shows the property basically identical with above-described embodiment after tested.
Exemplary description has been done to the present invention above, it should explanation, in the situation for the core for not departing from the present invention
Under, any simple deformation, modification or other skilled in the art can not spend the equivalent substitution of creative work equal
Fall into protection scope of the present invention.
Claims (10)
- A kind of 1. Tea Caffeine green turnip ferment Traditional Chinese medicine potion, it is characterised in that the Tea Caffeine green turnip ferment Chinese medicine Potus is prepared by the fermentation of tea leaching liquor, black tea coffee alkali extracting solution, green turnip, chlorella algae powder, zymophyte and sucrose；Its In,The preparation method of the Tea Caffeine green turnip ferment Traditional Chinese medicine potion, is prepared as steps described below：Step 1, low caffeine black tea is put into water-bath in 70~90 DEG C of water and extracts 10~60min, filtering, obtain tea extraction Liquid, wherein, in parts by mass, the ratio of the low caffeine black tea and water is 1：(45~55)；Step 2, high caffeine black tea is crushed, crosses 16~50 mesh sieves, the high caffeine black tea is extracted with circumfluence distillation device Caffeine, obtain black tea coffee alkali extracting solution, it is standby；Wherein, extracting parameter is：In parts by mass, the high caffeine is red The ratio of tea and water is 1：(10~20), return time are 30~90min；Step 3, green turnip is put into 80~100 DEG C of water and boils 5~15min, pull green turnip out, be cooled to 20~25 DEG C of room temperature, The green turnip is added in the tea leaching liquor, is mixed with beating, obtains slurry；Wherein, in parts by mass, the green turnip Ratio with tea leaching liquor is (1~3)：(11~13)；Step 4, chlorella algae powder, zymophyte and sucrose are added in the slurry, stirring makes it well mixed, uniformly after mixing Fermented 5~8 days at 22~32 DEG C, the slurry after fermentation is filtered, fermented liquid is obtained after filtering；Wherein, wherein, it is described Zymophyte is bread microzyme, ferment bacillus or the Mixed Microbes of the bread microzyme and ferment bacillus, bread ferment Female bacterium is added in the form of dry bacterium powder, and ferment bacillus is added in the form of the nutrient solution of ferment bacillus, in ferment The concentration of ferment bacillus is 1.2 × 10 in the nutrient solution of plain bacillus9CFU/mL；Mixed Microbes are entered in the form of mixed bacteria liquid Row addition, in mixed bacteria liquid, the mass fraction of bread microzyme is consistent with the volume parts of the nutrient solution of ferment bacillus, It is 1g per a mass fraction, is 1mL per a volume parts；In parts by mass, the slurry, chlorella algae powder and sucrose Than for 100：(3~7)：(2~4)；Wherein, based on parts by volume, the ratio of the slurry and the nutrient solution of the ferment bacillus is 100：(0.7~1.2), is pressed Mass parts meter, the ratio of the slurry and bread microzyme is 100：(0.7~1.2)；Based on parts by volume, the ratio of the slurry and mixed bacteria liquid is 100：(0.7~1.2)；Step 5, the black tea coffee alkali extracting solution described in step 2 is added in described fermented liquid in step 4, is mixed to equal It is even, obtain Tea Caffeine green turnip ferment Traditional Chinese medicine potion；Wherein, in parts by mass, the black tea coffee alkali extracting solution and step The ratio of green turnip described in 3 is (1~3)：(1~3).
- 2. Tea Caffeine green turnip ferment Traditional Chinese medicine potion according to claim 1, it is characterised in that in step 1, institute The content for stating caffeine in low caffeine black tea is 27~33mg/g, preferably 27~32mg/g；In step 1, by low coffee Alkali black tea is put into water-bath in 75~85 DEG C of water and extracts 15~40min, filtering, obtains tea leaching liquor.
- 3. Tea Caffeine green turnip ferment Traditional Chinese medicine potion according to claim 1, it is characterised in that in step 2, institute The content for stating caffeine in high caffeine black tea is 33~46mg/g, preferably 43~46mg/g；In step 3, by mass parts Meter, the ratio of green turnip and the tea leaching liquor is (7~12)：(57~61).
- 4. Tea Caffeine green turnip ferment Traditional Chinese medicine potion according to claim 1, it is characterised in that in step 4, Chlorella algae powder, zymophyte and sucrose are added in 20~32 DEG C of the slurry；In step 4, the slurry is filtered Sieve mesh number is 16~100 mesh；In step 4, the ferment bacillus is bacillus subtilis through strain idenfication, and by bacterium Strain Bacillus subtilis Liu-3 are stored in China Committee for Culture Collection of Microorganisms's common micro-organisms center, bacterium Kind preservation day：2013-08-15, culture presevation number are：CGMCC No.8026.
- 5. Tea Caffeine green turnip ferment Traditional Chinese medicine potion according to claim 1, it is characterised in that the step 5 it Afterwards, the Tea Caffeine green turnip ferment Traditional Chinese medicine potion is sterilized：In 100 DEG C of 8~15min of water-bath, room is naturally cooled to 20~25 DEG C of temperature.
- 6. a kind of preparation method of Tea Caffeine green turnip ferment Traditional Chinese medicine potion, it is characterised in that made as steps described below It is standby：Step 1, low caffeine black tea is put into water-bath in 70~90 DEG C of water and extracts 10~60min, filtering, obtain tea extraction Liquid, wherein, in parts by mass, the ratio of the low caffeine black tea and water is 1：(45~55)；Step 2, high caffeine black tea is crushed, crosses 16~50 mesh sieves, the high caffeine black tea is extracted with circumfluence distillation device Caffeine, obtain black tea coffee alkali extracting solution, it is standby；Wherein, extracting parameter is：In parts by mass, the high caffeine is red The ratio of tea and water is 1：(10~20), return time are 30~90min；Step 3, green turnip is put into 80~100 DEG C of water and boils 5~15min, pull green turnip out, be cooled to 20~25 DEG C of room temperature, The green turnip is added in the tea leaching liquor, is mixed with beating, obtains slurry；Wherein, in parts by mass, the green turnip Ratio with tea leaching liquor is (1~3)：(11~13)；Step 4, chlorella algae powder, zymophyte and sucrose are added in the slurry, stirring makes it well mixed, uniformly after mixing Fermented 5~8 days at 22~32 DEG C, the slurry after fermentation is filtered, fermented liquid is obtained after filtering；Wherein, wherein, it is described Zymophyte is bread microzyme, ferment bacillus or the Mixed Microbes of the bread microzyme and ferment bacillus, bread ferment Female bacterium is added in the form of dry bacterium powder, and ferment bacillus is added in the form of the nutrient solution of ferment bacillus, in ferment The concentration of ferment bacillus is 1.2 × 10 in the nutrient solution of plain bacillus9CFU/mL；Mixed Microbes are entered in the form of mixed bacteria liquid Row addition, in mixed bacteria liquid, the mass fraction of bread microzyme is consistent with the volume parts of the nutrient solution of ferment bacillus, It is 1g per a mass fraction, is 1mL per a volume parts；In parts by mass, the slurry, chlorella algae powder and sucrose Than for 100：(3~7)：(2~4)；Wherein, based on parts by volume, the ratio of the slurry and the nutrient solution of the ferment bacillus is 100：(0.7~1.2),In parts by mass, the ratio of the slurry and bread microzyme is 100：(0.7~1.2)；Based on parts by volume, the ratio of the slurry and mixed bacteria liquid is 100：(0.7~1.2)；Step 5, the black tea coffee alkali extracting solution described in step 2 is added in described fermented liquid in step 4, is mixed to equal It is even, obtain Tea Caffeine green turnip ferment Traditional Chinese medicine potion；Wherein, in parts by mass, the black tea coffee alkali extracting solution and step The ratio of green turnip described in 3 is (1~3)：(1~3).
- 7. the preparation method of Tea Caffeine green turnip ferment Traditional Chinese medicine potion according to claim 6, it is characterised in that In step 1, the content of caffeine is 27~33mg/g, preferably 27~32mg/g in the low caffeine black tea；In step 1 In, low caffeine black tea is put into water-bath in 75~85 DEG C of water and extracts 15~40min, filtering, obtains tea leaching liquor.
- 8. the preparation method of Tea Caffeine green turnip ferment Traditional Chinese medicine potion according to claim 6, it is characterised in that In step 2, the content of caffeine is 33~46mg/g, preferably 43~46mg/g in the high caffeine black tea；In step 3 In, in parts by mass, the ratio of green turnip and the tea leaching liquor is (7~12)：(57~61).
- 9. the preparation method of Tea Caffeine green turnip ferment Traditional Chinese medicine potion according to claim 6, it is characterised in that In step 4, chlorella algae powder, zymophyte and sucrose are added in 20~32 DEG C of the slurry；In step 4, the slurry The sieve mesh number filtered is 16~100 mesh；In step 4, the ferment bacillus is withered grass gemma through strain idenfication Bacillus, and it is commonly micro- that bacterial strain Bacillus subtilis Liu-3 are stored in into China Committee for Culture Collection of Microorganisms Bio-Centers, culture presevation day：2013-08-15, culture presevation number are：CGMCC No.8026.
- 10. the preparation method of Tea Caffeine green turnip ferment Traditional Chinese medicine potion according to claim 6, it is characterised in that After the step 5, the Tea Caffeine green turnip ferment Traditional Chinese medicine potion is sterilized：In 100 DEG C of 8~15min of water-bath, Naturally cool to 20~25 DEG C of room temperature.
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Application publication date: 20171208 |