CN112370516A - A wall-broken Ganoderma spore powder product with active ingredients and its preparation method - Google Patents
A wall-broken Ganoderma spore powder product with active ingredients and its preparation method Download PDFInfo
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Abstract
The invention discloses a wall-broken ganoderma lucidum spore powder product with active ingredients, and particularly relates to the technical field of ganoderma lucidum spore products, which comprises the following raw materials: broken wall ganoderma lucidum spore powder, ganoderma lucidum fruiting body, selfheal, dendrobium officinale, astragalus membranaceus, gentian, curcuma zedoary and oil additive. According to the invention, the ganoderma lucidum fruiting body extract, the selfheal, the dendrobium officinale, the astragalus membranaceus, the gentian and the curcuma zedoary are added into the wall-broken ganoderma lucidum spore powder, so that the traditional Chinese medicines have synergistic effect, the activity of a wall-broken ganoderma lucidum spore powder product is fully improved, the treatment effect on a patient is good, the mixed powder of the wall-broken ganoderma lucidum spore powder is subjected to film coating in a coating machine by adopting an oil additive, and the oil additive contains wheat germ oil, coix seed oil, olive oil and sunflower seed oil, so that the oxidation resistance of the wall-broken ganoderma lucidum spore powder product can be effectively improved.
Description
Technical Field
The invention relates to the technical field of ganoderma lucidum spore powder products, in particular to a wall-broken ganoderma lucidum spore powder product with active ingredients and a preparation method thereof.
Background
The Ganoderma spore powder contains multiple unique components beneficial to human body. Firstly, the ganoderma lucidum polysaccharide has the functions of enhancing the immune system and preventing and treating tumors; reducing blood pressure and preventing cardiovascular epidemic diseases; stimulating the secretion of insulin and reducing the blood sugar concentration; accelerating blood microcirculation, improving oxygen supply capacity of blood, reducing ineffective oxygen consumption of organism in static state, eliminating in vivo free radicals, improving cell membrane sealing degree of organism, resisting radiation, improving DNA, RNA and protein synthesis ability of liver, bone marrow and blood, and prolonging life. Secondly, the ganoderic acid has the effects of improving microcirculation, reducing cholesterol and avoiding angiosclerosis; strengthening the functions of the liver, the spleen and the intestines and stomach and strengthening the operation of digestive organs. Thirdly, the natural organic germanium has the functions of enhancing the blood supply of human bodies, promoting blood metabolism, eliminating free radicals in the bodies and preventing cell aging; can extract electrons from cancer cells to reduce the potential of the cancer cells, thereby inhibiting the cancer cells from deteriorating and spreading. And the IV is adenosine, and has the effects of inhibiting platelet aggregation and preventing thrombosis. And the trace element selenium has the effects of preventing cancer, relieving pain and preventing prostate lesion, and can be used together with vitamin C to prevent heart disease. The ganoderma lucidum spore powder coagulates the essence of ganoderma lucidum, and is rich in various unique components beneficial to human bodies.
At present, the ganoderma lucidum spore powder products in the market of China mainly comprise: firstly, ganoderma lucidum spore powder has the defects that the ganoderma lucidum spore powder is not subjected to wall breaking treatment, is not easy to digest and absorb by human bodies and is extremely waste; secondly, wall-broken ganoderma lucidum spore powder has the defects that the effective components are easy to be oxidized, and the packaging and using requirements are strict; the ganoderma lucidum spore polysaccharide capsule has the defects of single effective component, unused other effective components and high price. Therefore, it is our aim to utilize the ganoderma lucidum spore powder with high efficiency and to make the ganoderma lucidum spore powder serve human beings to the maximum extent.
The existing wall-broken ganoderma lucidum spore powder is mostly used independently, the raw materials of the product are single, the activity of the wall-broken ganoderma lucidum spore powder is low, the wall-broken ganoderma lucidum spore powder is easy to oxidize in the using process, the requirements on packaging and use are high, and the use by people is inconvenient.
Disclosure of Invention
In order to overcome the above defects of the prior art, the embodiments of the present invention provide a wall-broken ganoderma lucidum spore powder product with active ingredients and a preparation method thereof, and the problems to be solved by the present invention are: how to improve the activity of the wall-broken ganoderma lucidum spore powder and improve the oxidation resistance of the wall-broken ganoderma lucidum spore powder.
In order to achieve the purpose, the invention provides the following technical scheme: a wall-broken ganoderma lucidum spore powder product with active ingredients comprises the following raw materials in parts by weight: 50-60 parts of wall-broken ganoderma lucidum spore powder, 10-15 parts of ganoderma lucidum fruiting body, 5-8 parts of selfheal, 6-10 parts of dendrobium officinale, 2-4 parts of astragalus membranaceus, 6-10 parts of felwort, 6-10 parts of curcuma zedoary and 120 parts of grease additive 100.
In a preferred embodiment, the feed comprises the following raw materials in parts by weight: 53-57 parts of sporoderm-broken ganoderma lucidum spore powder, 12-13 parts of ganoderma lucidum fruit body, 6-7 parts of selfheal, 7-9 parts of dendrobium officinale, 2-3 parts of astragalus membranaceus, 7-9 parts of felwort, 7-9 parts of curcuma zedoary and 115 parts of grease additive 105.
In a preferred embodiment, the feed comprises the following raw materials in parts by weight: 55 parts of wall-broken ganoderma lucidum spore powder, 12.5 parts of ganoderma lucidum fruiting body, 6.5 parts of selfheal, 8 parts of dendrobium officinale, 3 parts of astragalus membranaceus, 8 parts of felwort, 8 parts of curcuma zedoary and 110 parts of oil additive.
In a preferred embodiment, the wall-broken ganoderma lucidum spore powder is prepared by an ultralow-temperature physical wall-breaking method, and the ganoderma lucidum fruiting body is a ganoderma lucidum fruiting body which is not sprayed with the spore powder.
In a preferred embodiment, the grease additive comprises the following raw materials in parts by weight: 20-30 parts of wheat germ oil, 5-8 parts of pigskin glue, 10-15 parts of coix seed oil, 10-20 parts of olive oil, 10-20 parts of sunflower seed oil and 30-45 parts of water.
The invention also provides a preparation method of the wall-broken ganoderma lucidum spore powder product with the active ingredients, which comprises the following specific preparation steps:
the method comprises the following steps: weighing the wall-broken ganoderma lucidum spore powder, the ganoderma lucidum fruiting body, the selfheal, the dendrobium officinale, the astragalus membranaceus, the gentian, the curcuma zedoary and the grease additive in parts by weight for later use;
step two: pulverizing the weighed Ganoderma fruiting body, adding resolvase, performing enzymolysis at pH of 5-6 and 40-48 deg.C for 30-50min, adding 95 wt% ethanol-water solution into the zymolyte, reflux-extracting at 75-85 deg.C for 1-3 hr, filtering, concentrating the filtrate to obtain intermediate product, desalting and eluting the intermediate product with D101 macroporous adsorbent resin chromatographic column, collecting the eluted liquid, and removing organic phase to obtain Ganoderma triterpene extract;
step three: cleaning, draining and cutting the weighed prunella vulgaris, dendrobium officinale, astragalus membranaceus, gentian and curcuma zedoary, freezing at a low temperature, drying, putting into a grinder for superfine grinding, screening the ground powder with a 800-mesh sieve, and mixing with the weighed wall-broken ganoderma lucidum spore powder;
step four: adding water into the uniformly mixed powder obtained in the third step, decocting twice, filtering and collecting the decoction in the two steps, combining the collected decoction, concentrating under reduced pressure to obtain thick paste, adding 55-65% by volume of ethanol aqueous solution into the thick paste, stirring for 20-30min, standing for 20min, taking supernatant, recovering ethanol under reduced pressure to obtain extract, and placing the extract into a pulverizer for ultrafine grinding to obtain composite powder;
step five: putting the components in the grease additive into a mixing tank, uniformly stirring, then carrying out reduced pressure concentration and drying, obtaining grease additive thick paste after drying, putting the grease additive thick paste into a superfine pulverizer for pulverization, screening by using a 800-mesh sieve after pulverization, and fully mixing with the composite powder obtained in the fourth step after screening;
step six: and (4) drying and cooling the composite powder mixed in the fifth step in a helium tank, and bagging, sealing and packaging after cooling to obtain the wall-broken ganoderma lucidum spore powder product with high active ingredients.
In a preferred embodiment, the fat and oil additive is prepared by mixing the pigskin glue with water to form a suspension, and then adding the wheat germ oil, the olive oil, the coix seed oil and the sunflower seed oil and stirring uniformly.
In a preferred embodiment, the decomposing enzyme in the second step is a mixed enzyme of cellulase, hemicellulase and papain.
In a preferred embodiment, 5 times of water is added in the first decoction in the fourth step, the decoction temperature is 50-60 ℃, the steam pressure is less than or equal to 0.1Mpa, the decoction time is 2 hours, 3 times of water is added in the second decoction, the decoction temperature is 50-60 ℃, the steam pressure is less than or equal to 0.1Mpa, and the decoction time is 1 hour.
In a preferred embodiment, the eluent used in the second step is 94-96 wt% ethanol-water solution, the ratio of the eluent to the intermediate product is 400-1000mL:1g, and the flow rate of the eluent is 0.85-1.0 mL/min.
The invention has the technical effects and advantages that:
1. the wall-broken ganoderma lucidum spore powder product with active ingredients prepared by the raw material formula has the advantages of promoting lymphocyte proliferation, improving the phagocytic capacity and the killing power of macrophages, NK cells and T cells and the like by adding the ganoderma lucidum fruiting body extract, the selfheal, the dendrobium candidum, the astragalus, the gentian and the curcuma zedoary into the wall-broken ganoderma lucidum spore powder, improving the phagocytic capacity and the killing power of the macrophages, NK cells and T cells by the ganoderma lucidum fruiting body extract which is ganoderma triterpene, and having good treatment effect on scrofula, gall and acute mastitis gall by the dendrobium candidum with the effects of clearing heat and purging fire and reducing the stagnation, enhancing the immunity of human body, promoting the phagocytic function of macrophages of tumor-bearing animals, enhancing the proliferation and differentiation of T lymphocytes and the activity of NK cells, and synergistically acting the wall-broken ganoderma lucidum spore powder, the selfheal, the dendrobium candidum, the astragalus, the gentian and the curcuma zedo, the activity of the wall-broken ganoderma lucidum spore powder product is fully improved, and the treatment effect on patients is better;
2. according to the invention, the grease additive and the mixed powder of the wall-broken ganoderma lucidum spore powder are mixed, the pigskin glue has a reticular space structure in the treatment process and can be combined with wheat germ oil, coix seed oil, olive oil and sunflower seed oil and then coated on the outer side of the wall-broken ganoderma lucidum spore powder, the grease additive contains the wheat germ oil, the coix seed oil, the olive oil and the sunflower seed oil, the wall-broken ganoderma lucidum spore powder is coated by the grease additive, the oxidation resistance of the product can be effectively improved, the wheat germ oil, the olive oil and the pigskin glue have good oxidation resistance, and the functions of delaying senescence, improving immunity and the like, the coix seed oil has certain cachexia resistance and pain relieving effects, the sunflower seed oil contains a large amount of unsaturated fatty acids such as linoleic acid and the like which are necessary for a human body, and the regeneration and growth of human body cells can.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1:
the invention provides a wall-broken ganoderma lucidum spore powder product with active ingredients, which comprises the following raw materials in parts by weight: 50 parts of wall-broken ganoderma lucidum spore powder, 10 parts of ganoderma lucidum sporocarp, 5 parts of selfheal, 6 parts of dendrobium officinale, 2 parts of astragalus mongholicus, 6 parts of gentian, 6 parts of curcuma zedoary and 100 parts of grease additive.
In a preferred embodiment, the wall-broken ganoderma lucidum spore powder is prepared by an ultralow-temperature physical wall-breaking method, and the ganoderma lucidum fruiting body is a ganoderma lucidum fruiting body which is not sprayed with the spore powder.
In a preferred embodiment, the grease additive comprises the following raw materials in parts by weight: 20 parts of wheat germ oil, 5 parts of pigskin glue, 10 parts of coix seed oil, 10 parts of olive oil, 10 parts of sunflower seed oil and 30 parts of water.
The invention also provides a preparation method of the wall-broken ganoderma lucidum spore powder product with the active ingredients, which comprises the following specific preparation steps:
the method comprises the following steps: weighing the wall-broken ganoderma lucidum spore powder, the ganoderma lucidum fruiting body, the selfheal, the dendrobium officinale, the astragalus membranaceus, the gentian, the curcuma zedoary and the grease additive in parts by weight for later use;
step two: crushing the weighed ganoderma lucidum sporocarp, adding a resolvase into the crushed ganoderma lucidum sporocarp for enzymolysis for 40min at the temperature of 45 ℃ and the pH value of 5.5, adding 95 wt% of ethanol-water solution into the zymolyte, performing reflux extraction for 3h at the temperature of 80 ℃, filtering and concentrating filtrate to obtain an intermediate product, desalting and eluting the intermediate product in a D101 macroporous adsorption resin chromatographic column, collecting eluted liquid, and removing an organic phase to obtain ganoderma lucidum triterpenoid extracted from the ganoderma lucidum sporocarp;
step three: cleaning, draining and cutting the weighed prunella vulgaris, dendrobium officinale, astragalus membranaceus, gentian and curcuma zedoary, freezing at a low temperature, drying, putting into a grinder for superfine grinding, screening the ground powder with a 800-mesh sieve, and mixing with the weighed wall-broken ganoderma lucidum spore powder;
step four: adding water into the uniformly mixed powder obtained in the third step, decocting twice, filtering and collecting the decoction in the two steps, combining the collected decoction, concentrating under reduced pressure to obtain thick paste, adding 60% ethanol water solution by volume fraction into the thick paste, stirring for 30min, standing for 20min, taking supernate, recovering ethanol under reduced pressure to obtain extract, and placing the extract into a pulverizer for ultrafine grinding to obtain composite powder;
step five: putting the components in the grease additive into a mixing tank, uniformly stirring, then carrying out reduced pressure concentration and drying, obtaining grease additive thick paste after drying, putting the grease additive thick paste into a superfine pulverizer for pulverization, screening by using a 800-mesh sieve after pulverization, and fully mixing with the composite powder obtained in the fourth step after screening;
step six: and (4) drying and cooling the composite powder mixed in the fifth step in a helium tank, and bagging, sealing and packaging after cooling to obtain the wall-broken ganoderma lucidum spore powder product with high active ingredients.
In a preferred embodiment, the fat and oil additive is prepared by mixing the pigskin glue with water to form a suspension, and then adding the wheat germ oil, the olive oil, the coix seed oil and the sunflower seed oil and stirring uniformly.
In a preferred embodiment, the decomposing enzyme in the second step is a mixed enzyme of cellulase, hemicellulase and papain.
In a preferred embodiment, in the fourth step, 5 times of water is added in the first decoction, the decoction temperature is 55 ℃, the steam pressure is less than or equal to 0.1MPa, and the decoction time is 2 hours, and 3 times of water is added in the second decoction, the decoction temperature is 55 ℃, the steam pressure is less than or equal to 0.1MPa, and the decoction time is 1 hour.
In a preferred embodiment, the eluent used in the second step is 95 wt% ethanol-water solution, the ratio of the eluent to the intermediate product is 700mL:1g, and the flow rate of the eluent is 0.9 mL/min.
Example 2:
different from the embodiment 1, the material comprises the following raw materials in parts by weight: 60 parts of wall-broken ganoderma lucidum spore powder, 15 parts of ganoderma lucidum sporocarp, 8 parts of selfheal, 10 parts of dendrobium officinale, 4 parts of astragalus membranaceus, 10 parts of felwort, 10 parts of curcuma zedoary and 120 parts of grease additive.
Example 3:
different from the examples 1-2, the material comprises the following raw materials in parts by weight: 55 parts of wall-broken ganoderma lucidum spore powder, 12.5 parts of ganoderma lucidum fruiting body, 6.5 parts of selfheal, 8 parts of dendrobium officinale, 3 parts of astragalus membranaceus, 8 parts of felwort, 8 parts of curcuma zedoary and 110 parts of oil additive.
Respectively taking the wall-broken ganoderma lucidum spore powder product with the active ingredients prepared in the above examples 1-3, and the wall-broken ganoderma lucidum spore powder without other substances and a blank control group for testing, selecting 50 Balb/c female mice, wherein the female mice grow for five weeks and have the weight of 20-25g, and the tumor model is mouse liver cancer H22 tumor strain; the administration mode is oral; balb/c female mice were randomly divided into five groups, i.e., a group of examples, a group of three groups of examples, a group of control group one and a group of control group two, ten of which were administered once a day at 400mg/kg per administration.
The products produced in the embodiments 1, 2 and 3 are respectively selected in the first embodiment, the second embodiment and the third embodiment, the first control group adopts the wall-broken ganoderma lucidum spore powder without other substances, and the second control group adopts normal saline;
the experimental method comprises the following steps: subcutaneously inoculating 100 μ L of H22 liver cancer cells with the concentration of 5 × 107/mL; the administration is started 24 hours after the inoculation, and the administration is continuously carried out for 10 times; during the experiment, the body weight of the mice is weighed every other day, the growth condition of the tumor is observed, the experiment is observed till 15 days after inoculation, the tumor of the mice is weighed and the volume is measured, the tumor inhibition rate is calculated, and the tumor inhibition rate is (the average tumor weight of a blank control group-the average tumor weight of a treatment group)/the average tumor weight of the blank control group is multiplied by 100 percent, and the measurement results are shown as the following table I:
watch 1
As can be seen from the table one, compared with the wall-broken ganoderma lucidum spore powder product without other substances, the wall-broken ganoderma lucidum spore powder product with active ingredients produced by the invention has the tumor inhibition rate of up to 69.4 percent and better active ingredients, by adding the ganoderma lucidum fruit body extract, the selfheal, the dendrobium officinale, the astragalus, the gentian and the curcuma zedoary into the wall-broken ganoderma lucidum spore powder, the ganoderma lucidum fruit body extract is ganoderma lucidum triterpene, the ganoderma lucidum triterpene is the main ingredient in the wall-broken ganoderma lucidum spore powder, has the advantages of promoting the proliferation of lymphocytes, improving the phagocytic capacity and the killing power of macrophages, NK cells and T cells, and the like, the selfheal has the effects of clearing heat, purging pathogenic fire, eliminating stagnation and reducing swelling, has better treatment effect on scrofula, gall and acute mastitis, the dendrobium officinale can enhance the immunity of a human body, can promote the phagocytic function of macrophages of tumor-bearing animals, and enhance the proliferation, the wall-broken ganoderma lucidum spore powder, the selfheal, the dendrobium officinale, the astragalus, the gentian and the curcuma zedoary act synergistically, so that the activity of a wall-broken ganoderma lucidum spore powder product is fully improved, and the treatment effect on patients is better.
Example 4
In the preferable technical scheme, the invention provides a wall-broken ganoderma lucidum spore powder product with active ingredients, which comprises the following raw materials in parts by weight: 60 parts of wall-broken ganoderma lucidum spore powder, 15 parts of ganoderma lucidum sporocarp, 8 parts of selfheal, 10 parts of dendrobium officinale, 4 parts of astragalus membranaceus, 10 parts of felwort, 10 parts of curcuma zedoary and 120 parts of grease additive.
In a preferred embodiment, the wall-broken ganoderma lucidum spore powder is prepared by an ultralow-temperature physical wall-breaking method, and the ganoderma lucidum fruiting body is a ganoderma lucidum fruiting body which is not sprayed with the spore powder.
In a preferred embodiment, the grease additive comprises the following raw materials in parts by weight: 25 parts of wheat germ oil, 6.5 parts of pigskin glue, 12.5 parts of coix seed oil, 15 parts of olive oil, 15 parts of sunflower seed oil and 37.5 parts of water.
The invention also provides a preparation method of the wall-broken ganoderma lucidum spore powder product with the active ingredients, which comprises the following specific preparation steps:
the method comprises the following steps: weighing the wall-broken ganoderma lucidum spore powder, the ganoderma lucidum fruiting body, the selfheal, the dendrobium officinale, the astragalus membranaceus, the gentian, the curcuma zedoary and the grease additive in parts by weight for later use;
step two: crushing the weighed ganoderma lucidum sporocarp, adding a resolvase into the crushed ganoderma lucidum sporocarp for enzymolysis for 40min at the temperature of 45 ℃ and the pH value of 5.5, adding 95 wt% of ethanol-water solution into the zymolyte, performing reflux extraction for 3h at the temperature of 80 ℃, filtering and concentrating filtrate to obtain an intermediate product, desalting and eluting the intermediate product in a D101 macroporous adsorption resin chromatographic column, collecting eluted liquid, and removing an organic phase to obtain ganoderma lucidum triterpenoid extracted from the ganoderma lucidum sporocarp;
step three: cleaning, draining and cutting the weighed prunella vulgaris, dendrobium officinale, astragalus membranaceus, gentian and curcuma zedoary, freezing at a low temperature, drying, putting into a grinder for superfine grinding, screening the ground powder with a 800-mesh sieve, and mixing with the weighed wall-broken ganoderma lucidum spore powder;
step four: adding water into the uniformly mixed powder obtained in the third step, decocting twice, filtering and collecting the decoction in the two steps, combining the collected decoction, concentrating under reduced pressure to obtain thick paste, adding 60% ethanol water solution by volume fraction into the thick paste, stirring for 30min, standing for 20min, taking supernate, recovering ethanol under reduced pressure to obtain extract, and placing the extract into a pulverizer for ultrafine grinding to obtain composite powder;
step five: putting the components in the grease additive into a mixing tank, uniformly stirring, then carrying out reduced pressure concentration and drying, obtaining grease additive thick paste after drying, putting the grease additive thick paste into a superfine pulverizer for pulverization, screening by using a 800-mesh sieve after pulverization, and fully mixing with the composite powder obtained in the fourth step after screening;
step six: and (4) drying and cooling the composite powder mixed in the fifth step in a helium tank, and bagging, sealing and packaging after cooling to obtain the wall-broken ganoderma lucidum spore powder product with high active ingredients.
In a preferred embodiment, the fat additive is prepared by mixing the pigskin glue with water to form a suspension, adding the wheat germ oil, the olive oil, the coix seed oil and the sunflower seed oil, homogenizing, and placing the obtained fat additive into a spray gun of a coating machine.
In a preferred embodiment, the decomposing enzyme in the second step is a mixed enzyme of cellulase, hemicellulase and papain.
In a preferred embodiment, in the fourth step, 5 times of water is added in the first decoction, the decoction temperature is 55 ℃, the steam pressure is less than or equal to 0.1MPa, and the decoction time is 2 hours, and 3 times of water is added in the second decoction, the decoction temperature is 55 ℃, the steam pressure is less than or equal to 0.1MPa, and the decoction time is 1 hour.
In a preferred embodiment, the eluent used in the second step is 95 wt% ethanol-water solution, the ratio of the eluent to the intermediate product is 700mL:1g, and the flow rate of the eluent is 0.9 mL/min.
Example 5
Different from the embodiment 4, the grease additive comprises the following raw materials in parts by weight: 30 parts of wheat germ oil, 8 parts of pigskin glue, 15 parts of coix seed oil, 20 parts of olive oil, 20 parts of sunflower seed oil and 45 parts of water.
Experiments were carried out on the wall-broken ganoderma lucidum spore powder products with active ingredients prepared in the above examples 2, 4 and 5 and the wall-broken ganoderma lucidum spore powder without adding other substances: the test samples were divided into test group 1, test group 2, test group 3 and control group, test group 1 corresponded to the product obtained in example 2, test group 2 corresponded to the product obtained in example 4, and test group 3 corresponded to the product obtained in example 5, the samples were placed in transparent sealed ampoules, placed at 60 ℃ for 10 days, and the acid value and peroxide value of the samples were measured on day 0, day 5 and day 10, respectively, and the test results are shown in table two:
watch two
From the above table, the wall-broken ganoderma lucidum spore powder is mixed with the grease additive, the wall-broken ganoderma lucidum spore powder is placed for 10 days at the temperature of 60 ℃, the acid value and the peroxide value of the wall-broken ganoderma lucidum spore powder only have small amplitude change, which indicates that the grease additive can have strong oxidation resistance to the wall-broken ganoderma lucidum spore powder, the pigskin glue has a reticular space structure in the treatment process, can be combined with the wheat germ oil, the coix seed oil, the olive oil and the sunflower seed oil and then coated on the outer side of the wall-broken ganoderma lucidum spore powder, the grease additive contains the wheat germ oil, the coix seed oil, the olive oil and the sunflower seed oil, the wall-broken ganoderma lucidum spore powder is coated by the grease additive, the oxidation resistance of the product can be effectively improved, the wheat germ oil, the olive oil and the pigskin glue have good oxidation resistance, and have the functions of delaying senility, improving immunity and the like, and the coix seed oil has certain functions of, the sunflower seed oil contains a large amount of linoleic acid and other unsaturated fatty acids necessary for human body, and can promote regeneration and growth of human body cells.
And finally: the above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that are within the spirit and principle of the present invention are intended to be included in the scope of the present invention.
Claims (10)
1. A wall-broken ganoderma lucidum spore powder product with active ingredients is characterized in that: the feed comprises the following raw materials in parts by weight: 50-60 parts of wall-broken ganoderma lucidum spore powder, 10-15 parts of ganoderma lucidum fruiting body, 5-8 parts of selfheal, 6-10 parts of dendrobium officinale, 2-4 parts of astragalus membranaceus, 6-10 parts of felwort, 6-10 parts of curcuma zedoary and 120 parts of grease additive 100.
2. The product of wall-broken ganoderma lucidum spore powder with active ingredients according to claim 1, wherein the product comprises the following components in percentage by weight: the feed comprises the following raw materials in parts by weight: 53-57 parts of sporoderm-broken ganoderma lucidum spore powder, 12-13 parts of ganoderma lucidum fruit body, 6-7 parts of selfheal, 7-9 parts of dendrobium officinale, 2-3 parts of astragalus membranaceus, 7-9 parts of felwort, 7-9 parts of curcuma zedoary and 115 parts of grease additive 105.
3. The product of wall-broken ganoderma lucidum spore powder with active ingredients according to claim 1, wherein the product comprises the following components in percentage by weight: the feed comprises the following raw materials in parts by weight: 55 parts of wall-broken ganoderma lucidum spore powder, 12.5 parts of ganoderma lucidum fruiting body, 6.5 parts of selfheal, 8 parts of dendrobium officinale, 3 parts of astragalus membranaceus, 8 parts of felwort, 8 parts of curcuma zedoary and 110 parts of oil additive.
4. The product of wall-broken ganoderma lucidum spore powder with active ingredients according to claim 1, wherein the product comprises the following components in percentage by weight: the wall-broken ganoderma lucidum spore powder is prepared by an ultralow-temperature physical wall-breaking method, and the ganoderma lucidum fruiting body which is not sprayed with the spore powder is adopted.
5. The product of wall-broken ganoderma lucidum spore powder with active ingredients according to claim 1, wherein the product comprises the following components in percentage by weight: the grease additive comprises the following raw materials in parts by weight: 20-30 parts of wheat germ oil, 5-8 parts of pigskin glue, 10-15 parts of coix seed oil, 10-20 parts of olive oil, 10-20 parts of sunflower seed oil and 30-45 parts of water.
6. The method for preparing a sporoderm-broken ganoderma lucidum spore powder product with an active ingredient according to any one of claims 1 to 5, wherein the method comprises the following steps: the preparation method comprises the following specific steps:
the method comprises the following steps: weighing the wall-broken ganoderma lucidum spore powder, the ganoderma lucidum fruiting body, the selfheal, the dendrobium officinale, the astragalus membranaceus, the gentian, the curcuma zedoary and the grease additive in parts by weight for later use;
step two: pulverizing the weighed Ganoderma fruiting body, adding resolvase, performing enzymolysis at pH of 5-6 and 40-48 deg.C for 30-50min, adding 95 wt% ethanol-water solution into the zymolyte, reflux-extracting at 75-85 deg.C for 1-3 hr, filtering, concentrating the filtrate to obtain intermediate product, desalting and eluting the intermediate product with D101 macroporous adsorbent resin chromatographic column, collecting the eluted liquid, and removing organic phase to obtain Ganoderma triterpene extract;
step three: cleaning, draining and cutting the weighed prunella vulgaris, dendrobium officinale, astragalus membranaceus, gentian and curcuma zedoary, freezing at a low temperature, drying, putting into a grinder for superfine grinding, screening the ground powder with a 800-mesh sieve, and mixing with the weighed wall-broken ganoderma lucidum spore powder;
step four: adding water into the uniformly mixed powder obtained in the third step, decocting twice, filtering and collecting the decoction in the two steps, combining the collected decoction, concentrating under reduced pressure to obtain thick paste, adding 55-65% by volume of ethanol aqueous solution into the thick paste, stirring for 20-30min, standing for 20min, taking supernatant, recovering ethanol under reduced pressure to obtain extract, and placing the extract into a pulverizer for ultrafine grinding to obtain composite powder;
step five: putting the components in the grease additive into a mixing tank, uniformly stirring, then carrying out reduced pressure concentration and drying, obtaining grease additive thick paste after drying, putting the grease additive thick paste into a superfine pulverizer for pulverization, screening by using a 800-mesh sieve after pulverization, and fully mixing with the composite powder obtained in the fourth step after screening;
step six: and (4) drying and cooling the composite powder mixed in the fifth step in a helium tank, and bagging, sealing and packaging after cooling to obtain the wall-broken ganoderma lucidum spore powder product with high active ingredients.
7. The method for preparing the product of the sporoderm-broken ganoderma lucidum spore powder with the active ingredients according to claim 6, wherein the method comprises the following steps: the preparation method of the oil additive comprises the steps of mixing the pigskin glue with water to form a suspension, adding the wheat germ oil, the olive oil, the coix seed oil and the sunflower seed oil, and uniformly stirring.
8. The method for preparing the product of the sporoderm-broken ganoderma lucidum spore powder with the active ingredients according to claim 6, wherein the method comprises the following steps: in the second step, the decomposing enzyme adopts cellulase, hemicellulase and papain mixed enzyme.
9. The method for preparing the product of the sporoderm-broken ganoderma lucidum spore powder with the active ingredients according to claim 6, wherein the method comprises the following steps: in the fourth step, 5 times of water is added in the first decoction, the decoction temperature is 50-60 ℃, the steam pressure is less than or equal to 0.1Mpa, the decoction time is 2 hours, 3 times of water is added in the second decoction, the decoction temperature is 50-60 ℃, the steam pressure is less than or equal to 0.1Mpa, and the decoction time is 1 hour.
10. The method for preparing the product of the sporoderm-broken ganoderma lucidum spore powder with the active ingredients according to claim 6, wherein the method comprises the following steps: and the eluent adopted in the elution in the second step is 94-96 wt% of ethanol-water solution, the ratio of the eluent to the intermediate product is 400-1000mL:1g, and the flow rate of the eluent is 0.85-1.0 mL/min.
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| CN114514982A (en) * | 2021-12-17 | 2022-05-20 | 四川黑农夫农业科技开发有限公司 | Active storage and preservation method of health-preserving porridge material |
| CN115624178A (en) * | 2022-10-28 | 2023-01-20 | 浙江海兴药业有限公司 | Compound ganoderma lucidum extract spore powder and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106722875A (en) * | 2016-12-07 | 2017-05-31 | 盐城工学院 | The extracting method and Ganoderma lucidum of a kind of ganodenic acid |
| CN111643531A (en) * | 2020-07-10 | 2020-09-11 | 浙江海兴药业有限公司 | Compound ganoderma lucidum wall-broken spore powder and preparation method thereof |
-
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106722875A (en) * | 2016-12-07 | 2017-05-31 | 盐城工学院 | The extracting method and Ganoderma lucidum of a kind of ganodenic acid |
| CN111643531A (en) * | 2020-07-10 | 2020-09-11 | 浙江海兴药业有限公司 | Compound ganoderma lucidum wall-broken spore powder and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114514982A (en) * | 2021-12-17 | 2022-05-20 | 四川黑农夫农业科技开发有限公司 | Active storage and preservation method of health-preserving porridge material |
| CN115624178A (en) * | 2022-10-28 | 2023-01-20 | 浙江海兴药业有限公司 | Compound ganoderma lucidum extract spore powder and preparation method thereof |
| CN115624178B (en) * | 2022-10-28 | 2023-11-17 | 浙江海兴药业有限公司 | Composite ganoderma lucidum extract spore powder and preparation method thereof |
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