CN112956627A - Compound solid drink of chaga for women - Google Patents

Compound solid drink of chaga for women Download PDF

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Publication number
CN112956627A
CN112956627A CN202110037547.9A CN202110037547A CN112956627A CN 112956627 A CN112956627 A CN 112956627A CN 202110037547 A CN202110037547 A CN 202110037547A CN 112956627 A CN112956627 A CN 112956627A
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extract
parts
drying
concentrating
inonotus obliquus
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朱明会
张莹
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Shanxi Yunyidao Biotechnology Co ltd
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Shanxi Yunyidao Biotechnology Co ltd
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/385—Concentrates of non-alcoholic beverages
    • A23L2/39—Dry compositions
    • A23L2/395—Dry compositions in a particular shape or form
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/15—Vitamins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

Abstract

The betulin composite solid beverage suitable for women comprises the following components in parts by weight: 15-18 parts of inonotus obliquus extract, 16-20 parts of rhizoma polygonati extract, 28-32 parts of blueberry extract, 3-7 parts of aloe extract, 3-7 parts of angelica extract, 22-28 parts of inulin, 4-6 parts of vitamin C and 0.5-0.8 part of stevioside. The betulin composite solid beverage suitable for women is mainly prepared from natural plant extracts, has the effects of tonifying qi and blood, regulating menstruation, relieving pain, resisting aging and maintaining beauty for women, and is suitable for industrial production in the field of food.

Description

Compound solid drink of chaga for women
Technical Field
The invention relates to the field of functional foods, in particular to a betulin composite solid beverage suitable for women.
Background
Inonotus obliquus, the scientific name Inonotus obliquus, is a fungus parasitic on the subcutaneous surface of living standing trees such as white birch, silver birch, elm, and alder or on the dead trunk of felled trees. The obtained Fuscoporia obliqua polysaccharide, lanosterol and triterpenoid oxide, Fuscoporia obliqua alcohol and Fuscoporia obliqua rhzomorph, melanin and tannin compounds, lignin, etc. have wide medicinal value, and have effects of lowering blood sugar, resisting tumor, resisting virus, enhancing immunity, etc. The traditional Chinese medicine holds that the inonotus obliquus mainly strengthens the body resistance, and can tonify qi and nourish blood, nourish yin and promote the production of body fluid, invigorate the spleen and stomach, nourish the liver and kidney, and tranquilize the mind; at the same time, it can also eliminate pathogenic factors, and has the actions of clearing away heat and toxic material, soothing liver, relieving depression, promoting blood circulation and resolving hard mass.
Rhizoma Polygonati is sweet and neutral in taste, has effects of invigorating qi, nourishing yin, moistening lung, and invigorating kidney, and can be used for treating weakness of spleen and stomach, tiredness, debilitation, xerostomia, cough due to lung deficiency, essence and blood deficiency, and internal heat diabetes. The blueberry is rich in anthocyanin and fiber, and has the functions of improving eyesight, enhancing autoimmunity, enhancing memory, resisting oxidation, resisting aging and the like. Aloe has antibacterial, antiinflammatory, and skin caring effects, and radix Angelicae sinensis has effects in replenishing blood, promoting blood circulation, regulating menstruation, and relieving pain. The angelica has sweet taste and warm nature, enters liver, heart and spleen channels, and has the functions of enriching blood and activating blood, regulating menstruation and relieving pain, and relaxing bowel.
The modern life of fast rhythm and high pressure leads women to have the problems of menstrual disorder, deficiency of qi and blood, dark and dim face and the like, and how to regulate the female is an important problem.
Disclosure of Invention
In order to solve the existing problems, the invention provides the betulin composite solid beverage suitable for women, and the solid beverage has the functions of maintaining beauty and keeping young, resisting aging, tonifying qi and blood, regulating menstruation and relieving pain for women. The invention is realized by the following technical scheme.
The invention aims to provide a betulin composite solid beverage suitable for women, which comprises the following components in parts by weight:
15-18 parts of inonotus obliquus extract, 16-20 parts of rhizoma polygonati extract, 28-32 parts of blueberry extract, 3-7 parts of aloe extract, 3-7 parts of angelica extract, 22-28 parts of inulin, 4-6 parts of vitamin C and 0.5-0.8 part of stevioside.
Wherein the Fuscoporia obliqua extract is prepared from wild Fuscoporia obliqua or artificially cultured Fuscoporia obliqua mycelium.
Preferably, the inonotus obliquus composite solid beverage comprises the following components in parts by weight:
15 parts of inonotus obliquus extract, 18 parts of rhizoma polygonati extract, 27 parts of blueberry extract, 4.7 parts of aloe extract, 4.7 parts of angelica sinensis extract, 15.3 parts of inulin, 4.7 parts of vitamin C and 0.6 part of stevioside.
In addition, the invention also provides a preparation method of the betulin composite solid beverage suitable for women, which comprises the following steps:
(1) cleaning Chaba, drying, pulverizing, sieving, adding Chaba powder into an extraction kettle, adding 70% ethanol according to the weight ratio of 1:8-1:10 of the material liquid, extracting at 55-63 deg.C for 4-5 h, filtering, extracting the residue for the second time, concentrating the extractive solution, and drying to obtain Chaba extract.
(2) Removing impurities from rhizoma polygonati, cleaning, drying, crushing and sieving, putting rhizoma polygonati powder into an extraction kettle, adding distilled water according to the weight ratio of 1:3-1:5 of material liquid, soaking for 3-4h at 55-60 ℃, separating extract, performing secondary extraction, evaporating and concentrating, adding 3-5 times of 90% alcohol, standing for 3-4h, concentrating separated supernatant, washing lower substances with 90% alcohol, concentrating washing liquid again, mixing the two concentrates, and performing vacuum drying to obtain the rhizoma polygonati extract.
(3) Cleaning blueberries, pulping, putting pulp into an extraction kettle, and mixing the pulp according to a feed liquid weight ratio of 1:8-1:10 adding 70% acidic ethanol, soaking at 55-60 deg.C for 1-2h, centrifuging at 1200r/min for 20min, evaporating the supernatant, concentrating, and vacuum drying at 55 deg.C to obtain fructus Myrtilli extract.
(4) Cleaning Aloe, peeling, mashing to obtain slurry, vacuum evaporating at 40 deg.C for concentration, extracting with ethyl acetate at 55-60 deg.C for 3 times, discarding organic phase, combining three water phases, evaporating for concentration, dissolving the obtained concentrate with isobutanol at 70 deg.C, cooling to 4-5 deg.C, crystallizing, and drying the separated crystal to obtain Aloe extract.
(5) Drying and pulverizing radix Angelicae sinensis, adding 70% ethanol at a weight ratio of 1:1-1:1.5, soaking for 2-3 hr, placing in a percolation barrel, percolating at a speed of 1mL/min, collecting 18-20 times of percolate, recovering ethanol under reduced pressure, and drying the rest liquid to obtain radix Angelicae sinensis extract.
(6) And (2) uniformly mixing the inonotus obliquus extract obtained in the step (1), the polygonatum sibiricum extract obtained in the step (2), the blueberry extract obtained in the step (3), the aloe extract obtained in the step (4), the angelica sinensis extract obtained in the step (5), inulin, vitamin C and stevioside in proportion, performing wet granulation, drying, and performing size stabilization, sterilization and packaging to obtain the inonotus obliquus composite solid beverage.
The invention introduces the effects of each component:
the efficacy of the inonotus obliquus extract: the active ingredients in the Inonotus obliquus extract comprise Inonotus obliquus polysaccharide, water-soluble fiber, triterpenoid, SOD, multiple minerals, vitamins, etc., and have effects of resisting tumor, preventing and treating diabetes, preventing and treating hypertension, resisting aging, enhancing immunity, resisting inflammation, relieving pain, resisting virus, resisting platelet aggregation, protecting liver, and improving and preventing allergic skin.
The efficacy of the polygonatum extract is as follows: the active ingredients of the polygonatum extract comprise polygonatum polysaccharide, polygonatum oligosaccharide, nicotinic acid, quinone compounds and the like, have the effects of resisting pathogenic microorganisms such as staphylococcus aureus and herpes virus, reducing blood sugar and blood fat, improving atherosclerosis and enhancing cellular immune function, and are clinically used for treating pulmonary tuberculosis, ringworm, cold and heat deficiency of the old, poor appetite and weakness, weak bones and muscles, soreness and weakness of waist and knees, coronary heart disease, hyperlipidaemia, diabetes, chronic hepatitis, leukopenia, low immune function and the like.
The blueberry extract has the following effects: the blueberry extract mainly contains anthocyanin as an active ingredient, has the effects of protecting vision, preventing blindness, glaucoma, cataract and retinal hemorrhage, improving myopia, macular degeneration, diabetic retinopathy, retinitis pigmentosa, nyctalopia and the like, can increase the flexibility of capillary vessels, promote the expansion and flexibility of the blood vessels, prevent the blood vessels from being ruptured, has strong antioxidant capacity, can eliminate free radicals for hardening the blood vessels, and prevents arteriosclerosis.
Efficacy of aloe vera extract: aloe has antibacterial, antiinflammatory, toxic materials clearing away, wound healing promoting, stomach invigorating, laxative, anticancer, skin caring, and immunity enhancing effects. In beautifying skin, aloe has the functions of resisting allergy, softening skin, repeating acne, effectively inhibiting ultraviolet rays, preventing pigmentation, keeping skin white and fine, and delaying aging.
The efficacy of the angelica extract is as follows: the angelica has the effects of enriching blood and activating blood, regulating menstruation and relieving pain, and loosening the bowel to relieve constipation, and is mainly used for treating blood deficiency and chlorosis, dizziness and palpitation, irregular menstruation, amenorrhea and dysmenorrheal, deficiency-cold abdominal pain, rheumatic arthralgia, traumatic injury, carbuncle, cellulitis, sore injury, and constipation due to intestinal dryness in traditional Chinese medicine.
The efficacy of inulin is as follows: inulin can control blood sugar, reduce blood sugar, promote mineral absorption, regulate intestinal microbial flora, improve intestinal health, and prevent constipation; inhibiting the generation of toxic fermentation products, protecting liver, and preventing colon cancer; treating obesity, reducing weight; antidepression, etc.
Preferably, the Inonotus obliquus extract of the present invention is prepared using artificially cultured Inonotus obliquus mycelia. The preparation method of artificially cultured Inonotus obliquus mycelium comprises the following steps:
providing a corn mixture consisting of corn kernels, corn cobs and corn silk, wherein the corn mixture consists of the following components in parts by weight: 65-75% of corn kernels, 20-30% of corncobs and 3-7% of corn stigma;
providing rice bran;
the corn mixture and the rice bran form uniformly mixed culture medium powder, and the weight of the culture medium powder comprises the following components: 75-95% of corn mixture and 5-25% of rice bran;
adding water into the culture medium powder to form a packaged culture medium, wherein the weight of the added water is 50-150% of the weight of the culture medium powder;
inoculating the inonotus obliquus strain to the packaging culture medium for packaging culture, wherein the culture temperature is 18-30 ℃, and the culture time is 40-60 days;
and taking out all the encapsulated cultures and drying to obtain the biotransformation mycelium composition.
According to a preferred embodiment of the present invention, the corn mixture is composed of corn kernels, corn cobs and corn stigma of black corn crops, and the rice bran is millet rice bran. Compared with common light-colored corn, the black corn has advantages in the aspects of crude fiber, mineral substances (the content of iron is up to 20 percent), vitamin (such as VB1, VB2 and the like) component content and the like, which can cause that the black corn can generate strong synergistic promotion effect on the growth of inonotus obliquus mycelium under the condition of being matched with millet rice bran which has high fiber content, high porosity and difficult caking.
In addition, through years of research, the inventor believes that a proper amount of corn stigma, particularly black corn stigma, has the effect of drawing pindianjing on the efficient accumulation of active ingredients such as triterpenes in hyphae.
According to the present invention, the corn blend preferably comprises 70% corn kernels, 25% corn cobs and 5% corn silks by weight.
According to the invention, the composition of the culture medium powder is preferably 80-90% by weight of the corn mixture and 10-20% by weight of the rice bran.
According to the invention, the granularity of the culture medium powder is preferably 10-20 meshes.
In accordance with the present invention, the Inonotus obliquus strain is preferably an Inonotus obliquus (Fr) Pilat strain of the genus Fuscoporia of the family Polyporaceae.
With respect to female health. In traditional Chinese medicine, women usually take blood as the main part, and the importance of nourishing blood and enriching blood is emphasized. Qi is the commander of blood, blood is the mother of qi, qi circulation is the blood circulation, and qi stagnation is the blood stasis, so both qi and blood are emphasized. In addition, without removing excessive pathogenic heat, latent cold, food toxicity, water dampness and blood stasis, blood can not be enriched and blood can be nourished, but instead fire heat, etc. are promoted to promote dryness. Tonifying blood and moving qi, dispelling pathogenic heat, warming and latent cold, removing food toxicity, draining water and dampness, and removing blood stasis and blood, the other two herbs can complement each other.
Under the guidance of the theory of traditional Chinese medicine, the angelica, the rhizoma polygonati, the blueberries and the vitamin C in the formula of the solid beverage have the effects of tonifying qi and enriching blood, the stevia rebaudiana and the inulin have the effects of promoting gastrointestinal peristalsis and helping digestion, and the inonotus obliquus, the inulin and the aloe have the effect of removing grease, so that greasy taste caused by excessive nourishing is avoided. Through the optimized combination and synergistic effect of the components, the solid beverage provided by the invention has the effects of removing blood poison and damp poison in vivo, removing heat, tonifying deficiency, tonifying qi, moistening lung and enriching blood, thereby achieving the effects of resisting aging, maintaining beauty and keeping young.
The betulin composite solid beverage suitable for women is mainly prepared from natural plant extracts, has the effects of tonifying qi and blood, regulating menstruation, relieving pain, resisting aging and maintaining beauty for women, and is suitable for industrial production in the field of food.
Drawings
FIG. 1 is a graph comparing the scavenging capacity of the products and partial components of the examples for hydroxyl radicals.
FIG. 2 is a graph comparing the removal of DPPH by the products and partial compositions of the various examples.
Detailed Description
The technical solution of the present invention will be described in more detail and fully with reference to the following embodiments.
The main raw materials in each example were prepared by the following method:
(1) preparation of Inonotus obliquus extract: the extraction process comprises the following steps: cleaning and drying the inonotus obliquus, crushing and sieving the inonotus obliquus by a 60-mesh sieve, putting the inonotus obliquus powder into an extraction kettle, and mixing the raw materials according to a feed liquid weight ratio of 1:10 adding 70% ethanol, extracting at 60 deg.C for 4 hr, filtering, extracting the residue twice, concentrating the extractive solution, and drying to obtain Chaba extract.
(2) Preparing a polygonatum extract: removing impurities from rhizoma polygonati, cleaning, drying at 55 ℃, crushing, sieving with a 60-mesh sieve, putting rhizoma polygonati powder into an extraction kettle, and mixing the raw materials according to a feed liquid weight ratio of 1:5 adding distilled water, leaching at 60 ℃ for 3h, separating the extract, performing secondary extraction, combining the two extracts, evaporating and concentrating at 60 ℃, adding 4 times of 90% alcohol, standing for 4h, evaporating and concentrating the separated supernatant, washing the lower substance with 90% alcohol, evaporating and concentrating the washing solution at 60 ℃, mixing the two concentrates, and performing vacuum drying at 60 ℃ and 0.065MPa to obtain the sealwort extract.
(3) Preparation of the blueberry extract: cleaning blueberries, pulping, putting pulp into an extraction kettle, and mixing the pulp according to a feed liquid weight ratio of 1: adding 70% acidic ethanol into 10, soaking at 60 deg.C for 2h, centrifuging at 1200r/min for 20min, evaporating supernatant at 60 deg.C, concentrating, and vacuum drying at 60 deg.C under 0.06MPa to obtain fructus Myrtilli extract.
(4) Preparation of aloe extract: cleaning Aloe, peeling, mashing to obtain slurry, vacuum concentrating at 60 deg.C, extracting with ethyl acetate at 60 deg.C for 3 times, discarding organic phase, mixing three water phases, concentrating by evaporation, dissolving the obtained concentrate with isobutanol at 70 deg.C, cooling to 4 deg.C, crystallizing, drying the separated crystal, and pulverizing to obtain Aloe extract.
(5) Preparing an angelica extract: drying and pulverizing radix Angelicae sinensis, adding 70% ethanol at a feed liquid weight ratio of 1:1.5, soaking for 2.5h, placing in a percolation barrel, percolating at a speed of 1mL/min, collecting 20 times of percolate, recovering ethanol under reduced pressure, and drying the residual liquid at 60 deg.C to obtain radix Angelicae sinensis extract.
Inulin, vitamin C and stevioside, other ingredients, are commercially available.
Example 1
Inonotus obliquus extract: extracting with wild Chaba.
Proportioning: 16 parts of inonotus obliquus extract, 17 parts of rhizoma polygonati extract, 28 parts of blueberry extract, 4.7 parts of aloe extract, 4.7 parts of angelica sinensis extract, 24.3 parts of inulin, 4.7 parts of vitamin C and 0.6 part of stevioside.
And compounding to obtain the solid beverage EG.1.
Example 2
Inonotus obliquus extract: extracting with wild Chaba.
Proportioning: 15 parts of inonotus obliquus extract, 18 parts of rhizoma polygonati extract, 27 parts of blueberry extract, 4.7 parts of aloe extract, 4.7 parts of angelica sinensis extract, 15.3 parts of inulin, 4.7 parts of vitamin C and 0.6 part of stevioside.
And compounding to obtain the solid beverage EG.2.
Example 3
Inonotus obliquus extract: extracting with wild Chaba.
Proportioning: 16 parts of inonotus obliquus extract, 17 parts of rhizoma polygonati extract, 28 parts of blueberry extract, 4.7 parts of aloe extract, 4.7 parts of angelica sinensis extract, 24.3 parts of inulin, 4.7 parts of vitamin C and 0.6 part of stevioside.
And compounding to obtain the solid beverage EG.3.
Example 4
Inonotus obliquus extract: extracting with artificially cultured Inonotus obliquus mycelium.
Proportioning: 15 parts of inonotus obliquus extract, 18 parts of rhizoma polygonati extract, 27 parts of blueberry extract, 4.7 parts of aloe extract, 4.7 parts of angelica sinensis extract, 15.3 parts of inulin, 4.7 parts of vitamin C and 0.6 part of stevioside.
And compounding to obtain the solid beverage EG.4.
The artificially cultured Inonotus obliquus mycelium of example 4 was prepared by the following method comprising:
(1) culture substrate raw Material preparation
The black corn seeds are purchased from Chinese agricultural academy of sciences for field planting, and after the seeds are mature, the corn kernels, the corn cobs and the corn silk are respectively mixed according to the weight ratio of 70%, 25% and 5% and then crushed to form a black corn mixture for later use.
Millet bran is purchased from the market for standby.
Inonotus obliquus (Fr) Pilat strain of Inonotus obliquus (Fuscoporia obliqua) of Fuscoporia of Polyporaceae is purchased (from "northeast edible fungi research institute") and pre-cultured into mother strain for use.
(2) Artificial cultivation process
Mixing the black corn mixture and millet bran at a weight ratio of 80% to 20%, and adding purified water to obtain culture medium.
The resulting culture medium was bottled (500ml) and sterilized before inoculation with the strain stock.
Culturing at 25 deg.C for 55 days, taking out the dense mycelium block material in the bottle, and oven drying to obtain the biotransformation mycelium composition.
Oxidation resistance test
If the free radicals are generated too much or the scavenging speed is too slow, after reaching a certain amount in vivo, the free radicals can be continuously generated and cause biological effects, and macromolecular substances and various organelles can be attacked by the free radicals to cause the damage of the organism, accelerate the aging of the organism and cause various diseases.
The antioxidant capacity of the product is evaluated by measuring the clearance rate of hydroxyl free radical (. OH) and 1, 1-diphenyl-2-trinitrophenylhydrazine free radical (DPPH) in vitro.
Sample processing method
Weighing 5g of EG.1, EG.2, EG.3 and EG.4 respectively, diluting to 50mL with absolute ethyl alcohol, carrying out ultrasonic treatment for 30min, and filtering to obtain filtrate as mother liquor for later use.
(1) Ability to scavenge OH
Taking 0.1mL of mother liquor, adding 1.9mL of absolute ethanol into a 10mL test tube to prepare a working solution (1) to be detected of the sample#). Simultaneously, 2mL of the mother liquor (2) was added to each of the two other tubes#) And 2mL of absolute ethanol (0)#) And the solution is used as a solution to be detected.
Adding 1mL of FeSO with the concentration of 6mmol/L into each tube of solution to be detected respectively4Mixing the solution with 1mL of 6mmol/L salicylic acid solution, adding 0.2mL of 8mmol/L H2O2Initiation of the reaction (2)#0.2mL of distilled water was used in place of H2O2). Reacting in water bath at 37 deg.C for 30min, centrifuging at 4000rpm for 10min, collecting supernatant, and measuring absorbance at 510nm wavelength.
Hydroxyl radical clearance calculation formula: clearance (%) ═ a0-(A1-A2)]/A0×100
A02mL of absolute ethanol and 1mL of FeSO41mL salicylic acid, 0.2mL H2O2Absorbance of the reaction.
A1-2 mL sample with 1mL FeSO41mL salicylic acid, 0.2mL H2O2Absorbance of the reaction.
A2-2 mL sample with 1mL FeSO4Absorbance of the reaction was 1mL of salicylic acid and 0.2mL of distilled water.
(2) Ability to scavenge DPPH
Taking 0.1mL of mother liquor, adding 1.9mL of absolute ethanol into a 10mL test tube to prepare a working solution (1) to be detected of the sample#). Simultaneously, 2mL of the mother liquor (2) was added to each of the two other tubes#) And 2mL of absolute ethanol (0)#) And the solution is used as a solution to be detected.
And (3).0mL of 0.1mmol/L DPPH-ethanol solution was added to each tube of the test solution (2)#3mL of absolute ethyl alcohol) are used, the mixture is uniformly mixed, and the mixture is subjected to light-shielding reaction for 30min and then absorbs at the wavelength of 517 nm.
DPPH free radical clearance calculation formula: clearance (%) ═ a0-(A1-A2)]/A0×100
A0Absorbance of 2mL absolute ethanol reacted with 3mL DPPH solution.
A1Absorbance of 2mL of sample reacted with 3mL of DPPH solution.
A2Absorbance of 2mL sample reacted with 3mL absolute ethanol.
Control experiment
Processing partial components (the inonotus obliquus extract, the blueberry extract, the aloe extract and the vitamin C) according to the mixture ratio of each embodiment to ensure that the concentration of the mother liquor of the solution to be tested of each component is consistent with the mixture ratio. Thereafter, OH and DPPH clearance of each fraction was examined as described above.
Results of the experiment
As shown in FIGS. 1 and 2, the products obtained in each example have good antioxidant ability, and particularly, the solid beverage prepared by using artificially cultured Inonotus obliquus mycelium in example 4 has the best antioxidant ability.
The above description is only an embodiment of the present invention, and is not intended to limit the present invention, and all equivalent changes and modifications according to the spirit of the present invention are within the scope of the technical protection scheme of the present invention.

Claims (2)

1. The betulin composite solid beverage suitable for women comprises the following components in parts by weight:
15-18 parts of inonotus obliquus extract, 16-20 parts of rhizoma polygonati extract, 28-32 parts of blueberry extract, 3-7 parts of aloe extract, 3-7 parts of angelica extract, 22-28 parts of inulin, 4-6 parts of vitamin C and 0.5-0.8 part of stevioside.
2. A method for preparing the betulin complex solid beverage as set forth in claim 1, comprising the steps of:
(1) cleaning Chaba, drying, pulverizing, sieving, adding Chaba powder into an extraction kettle, adding 70% ethanol according to the weight ratio of 1:8-1:10 of the material liquid, extracting at 55-63 deg.C for 4-5 h, filtering, performing secondary extraction on the residue, concentrating the extract, and drying to obtain Chaba extract;
(2) removing impurities from rhizoma polygonati, cleaning, drying, crushing and sieving, putting rhizoma polygonati powder into an extraction kettle, adding distilled water according to the weight ratio of 1:3-1:5 of material liquid, soaking for 3-4h at 55-60 ℃, separating extract, performing secondary extraction, evaporating and concentrating, adding 3-5 times of 90% alcohol, standing for 3-4h, concentrating separated supernatant, washing lower substances with 90% alcohol, concentrating washing liquid again, mixing the two concentrates, and performing vacuum drying to obtain rhizoma polygonati extract;
(3) cleaning blueberry, pulping, placing the pulp into an extraction kettle, adding 70% acidic ethanol according to the weight ratio of the material liquid of 1:8-1:10, soaking at 55-60 ℃ for 1-2h, centrifuging at 1200r/min for 20min, evaporating and concentrating the supernatant, and vacuum drying at 55 ℃ to obtain a blueberry extract;
(4) cleaning aloe, peeling, mashing to obtain pulp, concentrating under reduced pressure at 40 deg.C, extracting with ethyl acetate at 55-60 deg.C for 3 times, discarding organic phase, mixing three water phases, concentrating by evaporation to obtain concentrate, dissolving with isobutanol at 70 deg.C, cooling to 4-5 deg.C, crystallizing, and drying to obtain aloe extract;
(5) drying and pulverizing radix Angelicae sinensis, adding 70% ethanol according to the weight ratio of the material liquid of 1:1-1:1.5, soaking for 2-3h, placing in a percolation barrel, percolating at 1mL/min, collecting percolate of 18-20 times, recovering ethanol under reduced pressure, and drying the residual liquid to obtain radix Angelicae sinensis extract;
(6) and (2) uniformly mixing the inonotus obliquus extract obtained in the step (1), the polygonatum sibiricum extract obtained in the step (2), the blueberry extract obtained in the step (3), the aloe extract obtained in the step (4), the angelica sinensis extract obtained in the step (5), inulin, vitamin C and stevioside according to a ratio, performing wet granulation, drying, and performing size stabilization, sterilization and packaging to obtain the inonotus obliquus compound solid beverage.
CN202110037547.9A 2021-01-12 2021-01-12 Compound solid drink of chaga for women Pending CN112956627A (en)

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Application publication date: 20210615