KR20130074118A - Ginseng prosapogenin high concentration containing ginseng berry preparation using sonication and process for thereof - Google Patents

Abstract

PURPOSE: A method for manufacturing a ginseng fruit formulation containing high concentration ginseng prosapogenin by sonication is provided to obtain high concentration ginsenoside Rg_2, Rh_1, and F_4 when making red ginseng. CONSTITUTION: A method for manufacturing a ginseng fruit formulation containing a prosapogenin ingredient comprises the steps of: treating a ginseng fruit by sonication at 80-120 deg. C for 1-60 minutes; and drying the treated ginseng fruits. The ginseng fruit is ginseng or an alcohol extract of a ginseng fruit. The ginseng fruit is treated by adding distilled water and performing sonication.

Description

The present invention relates to a ginseng prosapogenin high concentration containing ginseng berry preparation using sonication and a process for preparing the same,

The present invention relates to a ginseng fruit preparation and a method for producing the same. Especially, the content of prosapogenin, which is a functional substance produced by heat during the production of red ginseng, can be increased by ultrasonication of ginseng fruit.

Korean Ginseng ( Panax ginseng ) is a representative product of Korea, which is a summary of Daebo- boo , which is handled by products of sacred Maternity, menstrual cycle , taste is slightly warm, KOREA INSAM is a physiologically active substance which contains 30 kinds of ginseng saponins including ginsenoside Rb ₂ having anti-diabetic action, polyacetylene having anticancer action, phenolic compound having antioxidant activity, ginseng protein having radiation- , An acidic polysaccharide having an immunoregulatory action, and the like. In particular, Korean ginseng contains a larger amount of phenolic compounds, polyacetylenes and acidic polysaccharides than Panax quinquefolium , so that stronger physiological activity is expected. The ginseng saponin, which is known as the main pharmacological component of Korean ginseng, is called Jinseong - said, and its chemical structure has been confirmed by the Shibata group of Tokyo University. As such, the ginseng saponin contained in KOREA INSAM is 30 species, 14 species of western ginseng and 13 species of Panax notoginseng ) than 15 species. The ginsenosides are broadly divided into the protopanaxdiol group and the protopanaxtriol group. The major component of the protopanaxadiol group is ginsenoside Rb ₁ , which exhibits a central nervous inhibitory action , A major component of the protopanaxyl triol group, is shown to exhibit central nerve stimulation by ginsenoside Rg ₁ and is believed to contribute mainly to the adaptogen activity of Korean ginseng.

The content ratio (Rb ₁ / Rg ₁ ) of ginsenoside Rb ₁ to ginsenoside Rg ₁ (Rb ₁ / Rg ₁ ) in the Korean ginseng was 1.96 and that of protopanaxadiol and protopanaxatriol 3.14, showing a more balanced and more exciting, rather than exciting, visual action, as compared with 2.48 and 25.96 of the western ginseng exhibiting excessive central nervous inhibitory action. Therefore, the Korean ginseng has a pharmacological effect, and it has a true meaning, that is, the ginsenoside Rb ₁ of the inner spirit action and the ginsenoside Rg ₁ of the inner gesture are balanced, It seems to be the best tonic medicine of modern people in.

The pharmacological activities of Korean ginseng so far confirmed include reinforcement of heart and blood vessels, recovery of blood function, improvement of blood flow, prevention of atherosclerosis and hypertension, strengthening of gastrointestinal function, promotion of liver function and elimination of hangover, The present invention relates to a pharmaceutical composition for treating or preventing anxiety or depression of a person suffering from or at risk of developing a disease, Enhancement of immunity, inhibition of AIDS virus proliferation, and promotion of protein synthesis ability.

Also, it is said that dried red ginseng, peel, and roots are dried and dried naturally in the sunlight. Particularly, red ginseng is attracted attention as a special feature of red ginseng because of its specific action such as ginsenosides Rg _{3 ,} Rg _{5 , and} Rh _{1 ,} which are generated by heat and have a small amount _{, have} antitumor activity, antitumor activity, hypotensive action and antioxidant action .

Ginseng has been regarded as the best brand for a long time, but now it has a market share of 3% in the world ginseng market. In order to innovate such ginseng industry, it is necessary to develop high value-added ginseng products Do.

Nevertheless, the ginseng and red ginseng preparations so far have been developed at the level of the tonic to form the mainstream extract. However, in order to develop more specialized and functional ginseng preparations, it is essential to develop ginseng preparations containing a high concentration of a functional substance with a high level of physiological activity and safety.

For these reasons, recently, methods for making ginseng preparations containing a large amount of ginsenoside Rg ₃ having excellent effects among components of ginseng have been attempted.

According to the Shibata paper published in 1966, when saponin is hydrolyzed to a weak acid such as acetic acid, only glucosidic bonds of C-20 (ginsenosides Rb ₁ , Rb ₂ , Rc and Rd) (R & S) - Ginsenoside Rg ₃ ) was obtained, but this only resulted in the production of standard products. In addition, other researchers have used high-temperature treatment, which is a physical reaction, or enzymatic treatment, which is a biochemical reaction, in order to produce a ginseng preparation containing a large amount of ginsenoside Rg ₃ .

The ginseng is heat-treated at a high temperature of 120 to 180 ° C for 0.5 to 20 hours to obtain ginsenoside (Rg ₃ + Rg ₅ ). The ginseng is heated at a high temperature, for example, Wherein the ratio of (Rc + Rd + Rb ₁ + Rb ₂ ) is greater than or equal to 1, and a patent for beverage composition contained in the processed food (Korean Patent No. 192678) and saponin glucoside degrading enzyme (Korean Patent No. 329259) on a method for producing rare-anticancer saponins (Rh ₁ , Rh ₂ ) by hydrolysis of the saponin sugar, and they are commercially available as sansam and sinsam in the market. However, this method increases the efficacy, but it takes a long time to manufacture, requires special equipment such as a high-pressure heater, and particularly requires heating treatment at a temperature higher than normal processing temperature, so that ginseng is carbonized when mass- There are many operational difficulties in the manufacturing process.

(Japanese Patent No. 228510) and a blood vessel relaxant composition (Korean Patent No. 201585), ginsenosides Rg ₃ and / or Rg ₅ are produced under mild conditions instead of heat treatment at a high temperature, for example, An effective component such as Rg ₃ is produced in the same manner as in the reaction condition of heating treatment at a high temperature even when the acid is treated with a lower organic acid such as acetic acid, tartaric acid, oxalic acid or the like, but using a lower organic acid such as acetic acid or citric acid As a result of the experiment, it was confirmed that it was difficult to prepare ginseng preparations containing Rg ₃ and Rg ₅ , and a large amount of impurities were contained in the analysis of the components (Korean Patent No. 635025).

Examples of techniques using conventional vinegar include a ginseng preparation using vinegar and a preparation method thereof (Korean Patent No. 635025), a process for producing red ginseng vinegar mixed with ginseng and red ginseng (Korean Patent No. 244849), a method for producing ginseng vinegar (Korean Patent No. 344949), which is intended to produce vinegar by mixing only trace amounts of ginseng or red gins into vinegar, or a method for manufacturing ginseng preparations containing ginsenoside Rg _{3 at a} high concentration.

Therefore, the inventors of the present invention, while continuing to research to prepare a ginseng preparation containing a high concentration of functional substances, such as ginsenosides Rg ₂ , Rh ₁ and F ₄ , ginseng, unlike the processing method of heat treatment at high temperature To develop a ginseng fruit preparation containing high concentrations of prosapogenin components (for example, ginsenosides Rg ₂ , Rh ₁ and F ₄ ), which are functional substances produced in small amounts by heat when preparing red ginseng by ultrasonic irradiation. It is.

It is another object of the present invention to provide a ginseng fruit preparation containing the prosapogenin component at a high concentration, which is greatly enhanced in the efficacy of the ginseng, and a method for economical production thereof.

In order to accomplish the above object, the present invention provides a composition comprising a prosapogenin component Method for producing a ginseng fruit preparation containing, the step of sonicating the ginseng fruit for a time within the range of 1 minute to 60 minutes; And drying the sonicated ginseng fruit.

Here, the sonication is preferably sonicated for a time within a range of 30 to 50 minutes.

And, the ginseng fruit may be an alcohol extract of the ginseng fruit.

The ultrasonic treatment may be carried out at a temperature within a range of 80 ° C to 120 ° C.

In addition, the ultrasonic treatment is preferably put the ginseng fruit in distilled water and ultrasonic treatment, and the ginseng fruit in distilled water is more preferably 1 part by weight of ginseng fruit in 5 to 300 parts by weight of distilled water.

In addition, the ultrasonic treatment is preferably put the ginseng fruit in the vinegar within the range of pH 2 ~ 4, and ultrasonic treatment, and putting the ginseng fruit in the vinegar is more than 1 part by weight of the ginseng fruit in 5 to 300 parts by weight vinegar. desirable.

In addition, in the drying step, it is possible to concentrate and freeze-dried the sonicated ginseng fruit under reduced pressure.

On the other hand, another embodiment of the present invention is produced by the manufacturing method as described above, comprising a ginsenoside (Ginsenoside) characterized in that the high concentration of the prosapogenin component Containing ginseng fruit preparation.

Here, the ginsenosides are ginsenosides Rb ₁ , Rb ₂ , Ginsenoside Rg ₂ , for the sum of Rc, Rd, Re, Rf, Rg _{1 ,} Rg _2, Rg ₃ , Rg ₅ , Rg ₆ , Rh ₁ , Rh ₄ , Rk ₁ , Rk ₃ , F ₁ and F ₄ it is the Rh ₁ and F ₄ contained within the 65% to 85% by weight is preferred.

In addition, the ginseng fruit is ginseng fruit extract (extract), ginsenosides Rg ₂ , Rh ₁ and F ₄ Prosapogenin, characterized in that included in the range of 1% by weight to 10% by weight relative to the ginseng fruit extract Ingredients It may be a ginseng fruit preparation containing.

The details of other embodiments are included in the detailed description and drawings.

As described above, the present invention can increase the content of prosapogenin components (eg, ginsenosides Rg ₂ , Rh ₁ and F ₄ ), which are functional substances produced in small amounts by heat during the manufacture of red ginseng by sonicating ginseng fruit. It has an effect.

In addition, the present invention can provide a method for simply preparing a ginseng fruit preparation containing the high concentration of the above-mentioned prosapogenin ingredient by treating the ginseng fruit with distilled water or vinegar and subjecting it to ultrasonic treatment.

Figure 1 is an HPLC chromatogram of a standard authentic ginsenoside component.
FIG. 2 is an HPLC chromatogram of a ginsenoside component of a ginseng fruit preparation prepared by ultrasonic treatment for 10 minutes according to an embodiment of the present invention (Example 1)
3 is an HPLC chromatogram of the ginsenoside component of the ginseng fruit preparation prepared without the ultrasonic treatment according to Comparative Example 1 of the present invention (Comparative Example 1).

BRIEF DESCRIPTION OF THE DRAWINGS The present invention is capable of various modifications and various embodiments, and specific embodiments are illustrated in the drawings and will be described in detail in the detailed description. It is to be understood, however, that the invention is not to be limited to the specific embodiments, but includes all modifications, equivalents, and alternatives falling within the spirit and scope of the invention. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the present invention will be described in detail with reference to the accompanying drawings.

The terminology used in this application is used only to describe a specific embodiment and is not intended to limit the invention. Singular expressions include plural expressions unless the context clearly indicates otherwise. In the present application, the terms "comprises" or "having" and the like are used to specify that there is a feature, a number, a step, an operation, an element, a component or a combination thereof described in the specification, But do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, or combinations thereof.

The present invention relates to a method for treating ginseng fruit, comprising: sonicating ginseng fruit for a period of time ranging from 1 minute to 60 minutes; And drying the ultrasonic treated ginseng fruit. Containing ginseng extract of the present invention.

More specifically, the present invention relates to a method for producing a ginseng fruit preparation to be subjected to ultrasonic treatment, wherein a ginseng prosapogenin component (for example, ginsenoside Rg ₂ , Rh ₁ and F ₄ ), which is a functional substance, To produce a new concept of ginseng fruit preparation.

As described above, the present invention is characterized by maximizing the change of the active ingredient in the ginseng fruit and the content of the ginseng rare ingredient using ultrasonic waves.

The manufacturing method according to the present invention will be described in more detail as follows.

In the present invention, the ginseng fruit is subjected to ultrasonic treatment for a time within a range of 1 minute to 60 minutes.

The ginseng includes all kinds of ginseng belonging to the technical field of the present invention. The ginseng that can be used in the present invention is Korean ginseng ( Panax ginseng ), Panax quinquefolium , Panax notoginseng , Panax The present invention also encompasses a herbal medicine for a plant of the genus Panax such as Japonicum japonicum or Panax vietnamensis , for example, a ginseng, a ginseng, a japonica, a bamboo fruit, Based on facts confirmed from repeated experiments involving Preferably, the fruit of Korean ginseng can be used. The present invention is also applicable to the above-mentioned ginseng fruit (ginseng fruit extract).

In addition, the present invention is characterized in that the ginseng fruit is subjected to ultrasonic treatment for a time within a range of 1 minute to 60 minutes. The ultrasonic treatment can be performed by various treatment methods known in the art. For example, it is possible to use an ultrasonic generator of an oscillator 600W and a vibration unit 600W. The ultrasonic treatment is preferably performed for a time within a range of 1 minute to 60 minutes, more preferably 30 to 50 minutes, more preferably 35 to 45 minutes, .

One embodiment of the present invention, the ginseng fruit is an alcohol extract of the ginseng fruit, in the case of using the alcohol extract in this way has an effect that can be more effectively extract useful components from the ginseng fruit.

Another embodiment of the present invention, the ultrasonic treatment is performed at a high temperature within the range of 80 ℃ ~ 120 ℃, by extracting at a high temperature in this way can be prepared ginseng fruit formulations with increased drug efficacy.

Another embodiment of the present invention, in carrying out the ultrasonic treatment, is to put the ginseng fruit in distilled water or vinegar to treat the ultrasonic waves. In other words, the ultrasonic wave is more effectively and stably delivered to the ginseng fruit through distilled water or vinegar. When the ginseng fruit is sonicated in distilled water or vinegar, it is more preferable to add 1 part by weight of ginseng fruit to 5 to 300 parts by weight of distilled water or vinegar, but if the amount of distilled water or vinegar is less than the above range, the ultrasonic effect is insufficient. This is because when the above range is exceeded, changes in useful components may be caused by distilled water or vinegar.

Specifically, since the vinegar used in the present invention has the effect of preventing or eliminating the occurrence of lactic acid, which is a fatigue component in the body, smoothing metabolism, and generating adrenal cortex hormone, the present invention manufactured using such vinegar The ginseng preparation of may also contain more than 3% citric acid in the preparation to enhance and supplement the active ingredient effect in ginseng. Such vinegar is not particularly limited, and may be applied to all kinds of edible fermented vinegar, for example, brewed vinegar or a substitute thereof. As the brewing vinegar, grain vinegar such as rice vinegar, brown rice vinegar, malt vinegar or sake vinegar and persimmon vinegar, apple vinegar, grape vinegar, vinegar, tangerine vinegar, strawberry vinegar or plum vinegar can be used. For the citric acid effect, it is more preferable to use vinegar in the range of pH 2-4.

Then, the present invention is carried out by drying the ultrasonic treated ginseng fruit. The time and method of drying are not particularly limited, but the ultrasonic treated ginseng fruit can be dried in a vacuum dryer at a temperature within the range of 30 ° C to 50 ° C for a period of time ranging from 1 day to 3 days. In the drying step, it is more preferable that the ultrasonic treated ginseng fruit is concentrated under reduced pressure and freeze-dried.

As described above, when treated with distilled water or a vinegar having a pH of 2.0 to 3.0 at about 100 ° C for 1 minute to 60 minutes, ginsenoside components having high content of Rg ₂ , Rh ₁ and F ₄ A preparation can be prepared.

In addition, the manufacturing method of the present invention may further include a post processing step. The processing may be performed by a known method to prepare a processed ginseng extract. That is, the ginseng is extracted by using water, a lower alcohol such as methanol or ethanol, a lower ketone such as acetone or methyl ethyl ketone, a supercritical fluid or a mixed solvent thereof, To remove the solvent to prepare a dried powdery processed ginseng extract, that is, ginseng, in the form of a soft or dry extract.

In another embodiment of the present invention, there is provided a process for producing a prosapogenin composition, which comprises preparing a prosapogenin component which is produced by the above-described production method and comprises ginsenoside Containing ginseng fruit preparation.

Here, the ginsenosides are ginsenosides Rb ₁ , Rb ₂ , Rc, Rd, Re, Rf, Rg 1, Rg 2, Rg 3, Rg 5, Rg 6, Rh 1, Rh 4, Rk 1, Rk 3, F 1 and binary respect to the sum of F ₄ ginsenoside Rg _2, It is preferable that Rh ₁ and F ₄ are contained at a high concentration in the range of 65 wt% to 85 wt%.

In particular, the functional material Rg ₂ 1.0 to 2.3%, Rh ₁ 0.4 to 1.4%, and F _4: 0.3 to 1.3% at a high concentration.

That is, the ginseng fruit preparation according to the present invention, which is manufactured by the above method and contains ginseng prosapogenin at a high concentration, Improvement of metabolic syndrome, anticancer, blood pressure lowering, sexual function improvement, liver function improvement, brain cognitive function improvement, ADHD improvement, atopy and allergy improvement.

Furthermore, the total daily dose to be administered to the host at a single dose or two to three separate doses for clinical purposes of the ginseng fruit preparation of the present invention is preferably in the range of 1 to 50 mg per kg of body weight, May be varied depending on the particular compound being used, body weight, age, sex, health condition, diet, time of administration, method of administration, excretion rate, drug mix and severity of the disease.

The compounds of the present invention may be administered as injectable preparations and oral preparations as desired. Injectable preparations, for example sterile injectable aqueous or oleaginous suspensions, may be prepared according to the known art using suitable dispersing agents, wetting agents or suspending agents. Solvents that may be used herein include water, Ringer's solution and isotonic NaCl solution, and sterile, fixed oils are typically used as a solvent or suspending medium. Any non-irritating fixed oils, including mono-, di-glycerides, may be used for this purpose, and fatty acids such as oleic acid may be used in injectable preparations. Dosage forms for oral administration include capsules, tablets, pills, powders, granules and solutions, and particularly capsules, tablets and liquids are useful. Tablets and pills are preferably prepared as preservative. Solid and liquid dosage forms can be prepared by mixing the active ingredient with one or more inert diluents such as sucrose, lactose, starch and the like, lubricants such as magnesium stearate, talc, disintegration aids such as calcium CM, binders, fragrances, preservatives such as sodium benzoate , Sweeteners such as sugars or fructose, and surfactants. More specifically, a capsule may be prepared by adding starch and lactose as an excipient at a ratio of 7: 3 as an excipient, and adding magnesium stearate and talc at 3% or less for increasing fluidity. The starch and lactose Is added at a ratio of 7: 3, followed by tableting with calcium carbonate as a binder and disintegration aid. Flavor as a fragrance, sodium benzoate as a preservative, sugar or fructose as a sweetener, and a surfactant are added to a liquid Can be produced.

The present invention may be better understood by the following examples, which are for the purpose of illustrating the invention and are not intended to limit the scope of protection defined by the appended claims.

Manufacturing example  : Manufacture of ginseng fruit extract

2,500 ml of ethyl alcohol was added to 500 g of dried ginseng fruit, and the mixture was refluxed four times, filtered, and concentrated under reduced pressure to obtain ethyl alcohol ginseng extract.

Example  1 ~ 7: Preparation of ginseng fruit preparation using ultrasonic

Example  1: Ultrasonic treatment for 10 minutes with distilled water to prepare a ginseng fruit preparation

200 ml of distilled water was added to 1 g of ginseng fruit extract obtained in the above Preparation Example, and the mixture was put into a sonicator of 600 W, 600 W, and 100 캜, and sonicated for 10 minutes. Then, it was concentrated under reduced pressure and then lyophilized to obtain a brownish extract.

Example  2: Ultrasonic treatment for 20 minutes with distilled water to prepare a ginseng fruit preparation

The extract was obtained by the method of Example 1, except that the ultrasonic treatment was carried out for 20 minutes in the above Example 1.

Example  3: Ultrasonic treatment for 30 minutes with distilled water to prepare a ginseng fruit preparation

An extract was obtained by the method of Example 1, except that the ultrasonic treatment was performed for 30 minutes in the above-mentioned Example 1.

Example  4: Ultrasonic treatment with distilled water for 40 minutes to prepare a ginseng fruit preparation

An extract was obtained by the method of Example 1, except that the ultrasonic treatment was performed for 40 minutes in Example 1.

Example  5: Ultrasonic treatment for 50 minutes with distilled water to prepare a ginseng fruit preparation

An extract was obtained by the method of Example 1, except that the ultrasonic treatment was performed for 50 minutes in the above-mentioned Example 1.

Example  6: Ultrasonic treatment for 60 minutes with distilled water to prepare a ginseng fruit preparation

An extract was obtained by the method of Example 1, except that the ultrasonic treatment was performed for 60 minutes in Example 1.

Example  7: Ultrasonic treatment with vinegar for 40 minutes to prepare a ginseng fruit preparation

An extract was obtained in the same manner as in Example 1, except that the vinegar was used in place of the distilled water in Example 1 and ultrasonicated for 40 minutes.

Comparative example  1: Preparation of ginseng fruit preparation without ultrasonic treatment

To 500 g of dried ginseng fruit was added 2,500 ml of ethyl alcohol, and the mixture was refluxed twice, filtered and concentrated under reduced pressure to obtain ethyl alcohol ginseng fruit extract. Then, the obtained ginseng fruit extract was lyophilized to obtain a brownish extract.

Experimental Example  : HPLC Through law Gin Senocide  Component analysis

① Experimental method

50 ml of diethylether was added to 2 g of each of the ginseng fruit extract preparations obtained in Examples 1 to 7 and Comparative Example 1, and the mixture was centrifuged three times for 1 hour, and then the supernatant was removed.

Subsequently, 50 ml of butanol saturated with water was added to the obtained residue, and the mixture was extracted three times for 2 hours. The resulting mixture was centrifuged, and the supernatant was filtered and concentrated under reduced pressure to obtain crude saponin. The thus obtained crude saponin was quantified by HPLC method, and the results are shown in Tables 1 to 3 below.

② HPLC analysis conditions

The results obtained by the above-mentioned experimental method were directly compared with the specimens. That is, the content and the composition of ginseng saponin were repeatedly tested three times per each sample to confirm the reproducibility of the results. The product used was ginsenoside of 99% or more purity purchased from Chromadex (U.S.A.).

The HPLC apparatus used was a Waters 1525 binary HPLC system (Waters, USA) and a column was Eurospher 100-5 C18 (250 * 3 mm).

The mobile phase was acetonitrile (HPLC grade, Sigma, USA) and distilled water for HPLC. The ratio of acetonitrile was changed from 17% (0 min) to 30% (55 min), 40% (80 min), 60% % (140 min) and finally adjusted to 17%. The development temperature was measured at room temperature, the flow rate was 0.8 ml / min, and the chromatogram was detected at 203 nm using uv / vis Waters 2487 Dual λ Absorbance Detector (Waters, USA) detector.

BRIEF DESCRIPTION OF THE DRAWINGS Figure 1 is an HPLC chromatogram of a standard authentic ginsenoside component wherein the designation of each symbol represents the following ginsenoside;

  a: Ginsenoside Rg1, b: Ginsenoside Re, c: Ginsenoside Rf,

     d: Ginsenoside Rb1, e: Ginsenoside Rg2, f: Ginsenoside Rh1,

   g: Ginsenoside Rc, h: Ginsenoside Rb2, i: Ginsenoside F1,

    j: Ginsenoside Rd, k: Ginsenoside Rg6, l: Ginsenoside F4,

   m: Ginsenoside Rk3, n: Ginsenoside Rh4 o: Ginsenoside (20S) Rg3,

p: Ginsenoside (20R) Rg3, q: Ginsenoside Rk1, r: Ginsenoside Rg5.

FIG. 2 is an HPLC chromatogram of a ginsenoside component of a ginseng fruit preparation prepared by ultrasonic treatment for 10 minutes according to an embodiment of the present invention (Example 1) (Comparative Example 1) of the ginsenoside component of the ginseng fruit preparation prepared without the ultrasonic treatment.

As shown here, the sample was directly compared with the sample through HPLC, and the average was statistically calculated.

Table 1 below shows the ginsenoside content of the ginseng fruit extract obtained in Examples 1 to 7 and Comparative Example 1 described above.

As shown in Table 1, analysis of ginseng saponin ginsenosides Rb _1, Rb _2, Rc, Rd, Re, Rf, Rg _1, Rg _2, Rg _3, Rg _5, Rg _6, Rh _1, Rh ₄ , Rk ₁ , Rk ₃ , F ₁ and F ₄ , and the prosapogenin components (ginsenosides Rg ₂ , Rh ₁ and F ₄ ) useful in Examples 1 to 7 of the present invention were highly confirmed.

In particular, the content of ginsenoside Rg ₂ , which exhibits the effects of wrinkle-improving action and antithrombotic action, was highest at 2.278% in Example 4, and was higher than that of Example 2 (1.756% %), Respectively.

On the other hand, in the case of ginsenoside Rh ₁ in which ginsenoside Rg ₂ could be produced by hydrolysis, Example 4 showed the highest content as 1.35%, and Example 5 (0.96%), Example 2 (0.949% ), And the content of ginsenoside F ₄ was the highest as 1.21% in Example 4, and the highest content in Example 5 (0.856%) and Example 2 (0.778%) Respectively.

While the present invention has been particularly shown and described with reference to preferred embodiments thereof, it is to be understood that the invention is not limited to the disclosed embodiments, but, on the contrary, It will be apparent to those skilled in the art that changes may be made.

Claims (12)

Sonicating the ginseng fruit for a time within the range of 1 minute to 60 minutes; And
Drying the ultrasonically treated ginseng fruit; a prosapogenin component comprising a The manufacturing method of the ginseng fruit preparation containing.
The method of claim 1,
The sonication is characterized in that the prosapogenin component, characterized in that the ultrasonic treatment for a time within the range of 30 to 50 minutes The manufacturing method of the ginseng fruit preparation containing.
The method of claim 1,
The ginseng fruit is a prosapogenin component, characterized in that the alcohol extract of ginseng fruit The manufacturing method of the ginseng fruit preparation containing.
The method of claim 1,
The ultrasonication is carried out at a temperature in the range of 80 ℃ ~ 120 ℃ prosapogenin component, characterized in that The manufacturing method of the ginseng fruit preparation containing.
The method of claim 1,
The ultrasonication is a prosapogenin component, characterized in that the ginseng fruit is put in distilled water and sonicated. The manufacturing method of the ginseng fruit preparation containing.
The method of claim 5,
Putting the ginseng fruit in distilled water is a prosapogenin component, characterized in that 1 part by weight of ginseng fruit in 5 to 300 parts by weight of distilled water The manufacturing method of the ginseng fruit preparation containing.
The method of claim 1,
The ultrasonication is a prosapogenin component, characterized in that the ginseng fruit is put in vinegar within the pH 2 ~ 4 sonication The manufacturing method of the ginseng fruit preparation containing.
The method of claim 7, wherein
Putting the ginseng fruit in the vinegar is a prosapogenin component, characterized in that 1 part by weight of ginseng fruit is put in 5 to 300 parts by weight of vinegar The manufacturing method of the ginseng fruit preparation containing.
The method of claim 1,
In the drying step, the sosa-treated ginseng fruit is concentrated under reduced pressure and freeze-dried to a component of prosapogenin. The manufacturing method of the ginseng fruit preparation containing.
A prosapogenin component, which is prepared by the method according to any one of claims 1 to 9, comprising ginsenosides. Ginseng fruit preparation containing.
The method of claim 10,
The ginsenoside is ginsenoside Rb ₁ , Rb ₂ , Ginsenoside Rg ₂ , for the sum of Rc, Rd, Re, Rf, Rg _{1 ,} Rg _{2 ,} Rg ₃ , Rg ₅ , Rg ₆ , Rh ₁ , Rh ₄ , Rk ₁ , Rk ₃ , F ₁ and F ₄ Prosapogenin component, characterized in that Rh ₁ and F ₄ is included in the range of 65% by weight to 85% by weight Ginseng fruit preparation containing.
The method of claim 10,
The ginseng fruit is ginseng fruit extract (extract),
Ginsenosides Rg ₂ , Rh ₁ and F _{4 based} on the ginseng fruit extract X contained in the range of 1% by weight to 10% by weight of the prosapogenin component Ginseng fruit preparation containing.

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