KR101416674B1 - Ginseng prosapogenin high concentration containing American ginseng preparation using sonication and process for thereof - Google Patents

Abstract

The present invention relates to the use of ginsenosides Rg ₂ , Rg ₃ , Rg _{5 And,} Rk ₁ The present invention relates to a method for producing a high-concentration western-style ginseng preparation and a method for producing the same, and a method of ultrasonifying western ginseng or western ginseng extract at a temperature within a range of 1 to 60 minutes and a temperature within a range of 80 to 120 ° C, , Ginsenosides Rg ₂ , Rg ₃ , and Rg ₅ , which are small amounts of functional substances produced by heat during red ginseng production, _And, Rk ₁ In a high concentration, and a method for producing the same.

Description

TECHNICAL FIELD The present invention relates to a ginseng prosapogenin containing high concentration of ginseng prosapogenin and a method for producing the ginseng prosapogenin,

The present invention relates to a Western ginseng preparation and a method for producing the same. In particular, the content of prosapogenin, a functional substance produced by heat during the production of red ginseng, can be increased by ultrasonication of western ginseng.

Panax quinquefolium is a herbal medicine developed by immigrant Chinese immigrants who immigrated to the United States 200 years ago as an alternative to ginseng, and is also called American ginseng or flower ginseng. It was first listed as a Chinese herb medicine and a medicine as a raw liquid medicine for the first time in the 1700s Chinese Qing Dynasty. The name of the scientific name was named quinquefolium because there are five leaves (lobules) on the ground like ginseng.

Rg1, Rd, Rc, Rb1, Rb2, Rg3, and 24 (R) of ginsenosides Rg1, Rb2, Rg3, ) - pseudoginsenoside RT5, F11, and notoginsenoside K were isolated. Dou et al reported the isolation of ginsenoside Rg8. Wang et al. Reported the isolation of quinquenoside L3 and vinaginosenoside R3 from western ginseng leaves (leaves and stems). Wang et al. Isolated quinquenoside L9. Nakamura et al. Reported that floraquinquenosides A, B, C, D, E and others. Although some studies on the constituents and pharmacological actions of western ginseng have been conducted, it is necessary to develop western ginseng preparations containing high concentration of functional substances.

According to the Shibata paper published in 1966, when saponin is hydrolyzed to a weak acid such as acetic acid, only glucosidic bonds of C-20 (ginsenosides Rb ₁ , Rb ₂ , Rc and Rd) (R & S) - Ginsenoside Rg ₃ ) was obtained, but this only resulted in the production of standard products. In addition, other researchers have used high-temperature treatment, which is a physical reaction, or enzymatic treatment, which is a biochemical reaction, in order to produce a ginseng preparation containing a large amount of ginsenoside Rg ₃ .

The ginseng is heat-treated at a high temperature of 120 to 180 ° C for 0.5 to 20 hours to obtain ginsenoside (Rg ₃ + Rg ₅ ). The ginseng is heated at a high temperature, for example, Wherein the ratio of (Rc + Rd + Rb ₁ + Rb ₂ ) is greater than or equal to 1, and a patent for beverage composition contained in the processed food (Korean Patent No. 192678) and saponin glucoside degrading enzyme (Korean Patent No. 329259) on a method for producing rare-anticancer saponins (Rh ₁ , Rh ₂ ) by hydrolysis of the saponin sugar, and they are commercially available as sansam and sinsam in the market. However, this method increases the efficacy, but it takes a long time to manufacture, requires special equipment such as a high-pressure heater, and particularly requires heating treatment at a temperature higher than normal processing temperature, so that ginseng is carbonized when mass- There are many operational difficulties in the manufacturing process.

(Japanese Patent No. 228510) and a blood vessel relaxant composition (Korean Patent No. 201585), ginsenosides Rg ₃ and / or Rg ₅ are produced under mild conditions instead of heat treatment at a high temperature, for example, An effective component such as Rg ₃ is produced in the same manner as in the reaction condition of heating treatment at a high temperature even when the acid is treated with a lower organic acid such as acetic acid, tartaric acid, oxalic acid or the like, but using a lower organic acid such as acetic acid or citric acid As a result of the experiment, it was confirmed that it was difficult to prepare ginseng preparations containing Rg ₃ and Rg ₅ , and a large amount of impurities were contained in the analysis of the components (Korean Patent No. 635025).

Examples of techniques using conventional vinegar include a ginseng preparation using vinegar and a preparation method thereof (Korean Patent No. 635025), a process for producing red ginseng vinegar mixed with ginseng and red ginseng (Korean Patent No. 244849), a method for producing ginseng vinegar (Korean Patent No. 344949), which is intended to produce vinegar by mixing only trace amounts of ginseng or red gins into vinegar, or a method for manufacturing ginseng preparations containing ginsenoside Rg _{3 at a} high concentration.

The present inventors processing method for heat-treating the ginseng at a high temperature, of ohdeon preceding continued research in order to produce a ginseng preparation containing the functional material, such as ginsenoside Rg _2, Rg _3, Rg _{5 and,} Rk ₁ at a high concentration and , The present inventors have found that a western blodding agent containing a high concentration of a prosapogenin component (for example, ginsenoside Rg ₂ , Rg ₃ and Rg ₅ ), which is a functional substance produced by heat in the production of red ginseng, It is intended to develop.

It is another object of the present invention to provide a Western-style ginseng preparation containing a high concentration of the above-described prosapogenin, which is much more effective than conventional ginseng, and a method for economical production thereof.

In order to accomplish the above object, the present invention relates to a method for producing a microorganism, which comprises adding 1 part by weight of a western ginseng or a western ginseng extract into 5 to 300 parts by weight of distilled water and ultrasonic treatment at a temperature within a range of 90 to 110 ° C. ; And a step of concentrating and lyophilizing the ultrasound-treated western ginseng or western ginseng extract under a reduced pressure, wherein the content of ginsenosides Rg ₂ , Rg ₃ and Rg ₅ is increased.

Here, it is preferable to use an ultrasonic wave generator of 600 W to perform the ultrasonic wave processing.

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On the other hand, another embodiment of the present invention is a process for producing a ginsenoside Rb ₁ , Rb ₂ , Rc, Rd, Re, Rf, Rg 1, Rg 2, Rg 3, Rg 5, Rg 6, Rh 1, Rh 4, Rk 1, Rk 3, F 1 and binary respect to the sum of F ₄ ginsenoside Rg _2, Rg _{3, 5} and _Rg, Rk is ₁ is seoyangsam agents, characterized in that embedded into 1% by weight to 60% by weight.

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The details of other embodiments are included in the detailed description and drawings.

As described above, the present invention relates to a process for producing red ginseng, which comprises subjecting a Western ginseng or a Western ginseng extract to ultrasonic treatment to thereby obtain a content of a functional ingredient (for example, ginsenoside Rg ₂ , Rg ₃ and Rg ₅ ) There is an effect that can be extended.

In addition, the present invention can provide a method for easily producing a Western-style ginseng preparation containing a high concentration of the above-mentioned prosapogenin component by adding Western ginseng or Western ginseng extract into distilled water and subjecting the ginseng to ultrasonic treatment.

BRIEF DESCRIPTION OF THE DRAWINGS The present invention is capable of various modifications and various embodiments, and specific embodiments are illustrated in the drawings and will be described in detail in the detailed description. It is to be understood, however, that the invention is not to be limited to the specific embodiments, but includes all modifications, equivalents, and alternatives falling within the spirit and scope of the invention. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the present invention will be described in detail with reference to the accompanying drawings.

The terminology used in this application is used only to describe a specific embodiment and is not intended to limit the invention. The singular expressions include plural expressions unless the context clearly dictates otherwise. In the present application, the terms "comprises" or "having" and the like are used to specify that there is a feature, a number, a step, an operation, an element, a component or a combination thereof described in the specification, But do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, or combinations thereof.

The present invention relates to a method for producing a microorganism, which comprises ultrasonifying Western or ginseng extract for a time within a range of 1 minute to 60 minutes; And drying the ultrasonically treated western ginseng or western ginseng extract. Of the present invention.

That is, the present invention relates to a method for producing a Western-style ginseng preparation to be subjected to ultrasonic treatment, wherein a ginseng prosapogenin component (for example, ginsenoside Rg ₂ , Rg ₃ and Rg ₅ ) To produce a new concept of Western-style ginseng.

As described above, the present invention is characterized by maximizing the change of the active ingredient in Western ginseng and the content of rare ginseng ingredient by using ultrasonic waves.

The manufacturing method according to the present invention will be described in more detail as follows.

First, the present invention involves a step of ultrasonifying the extract of Western or Oriental ginseng for a period of time ranging from 1 minute to 60 minutes.

The western blotter includes all kinds of western blots belonging to the technical field of the present invention. The western hemp which can be used in the present invention includes the underground part of Panax quinquefolium such as Misham, White ginseng, Red ginseng, Red ginseng, Black ginseng, Black ginseng, Taekguk ginseng, Ginseng and their processed products, It is based on facts confirmed from relevant iterative experiments. In addition, the present invention is also applicable to the use of the above-mentioned seaweed ginseng extract (western ginseng extract).

In addition, the present invention is characterized in that the above-mentioned Western ginseng or Western ginseng extract is subjected to ultrasonic treatment for a time within a range of 1 minute to 60 minutes. The ultrasonic treatment can be performed by various treatment methods known in the art. For example, it is possible to use an ultrasonic generator of an oscillator 600W and a vibration unit 600W. The ultrasonic treatment is preferably performed for a time within a range of 1 minute to 60 minutes, more preferably 30 to 50 minutes, more preferably 30 to 40 minutes, .

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In another embodiment of the present invention, the ultrasound treatment is performed at a high temperature within a range of 90 ° C to 110 ° C, and thus the western blotting agent having an increased drug efficacy can be produced by extracting at such a high temperature.

In another embodiment of the present invention, in performing the ultrasonic treatment, the western or ginseng extract is put into distilled water and treated with ultrasonic waves. In other words, it is intended to transmit ultrasound more effectively and stably to western ginseng through distilled water. When the western or ginseng extract is added to distilled water and subjected to ultrasonic treatment, it is more preferable to add 1 part by weight of western ginseng or western ginseng extract to 5 to 300 parts by weight of distilled water. If the amount of distilled water is less than the above range, If it exceeds the range, the change of the useful component may be caused by the distilled water.

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Then, the present invention is carried out by drying the ultrasound-treated western ginseng or western ginseng extract. The time or method of drying is not particularly limited, but the ultrasound-treated western ginseng or western ginseng extract can be dried in a vacuum dryer at a temperature within a range of 30 ° C to 50 ° C for a period of time ranging from 1 day to 3 days. It is more preferable that the ultrasonically treated western ginseng or western ginseng extract is concentrated under reduced pressure and lyophilized.

Thus, when distilled water or a vinegar of pH 2.0 to 3.0 is added to Western or Oriental ginseng extract and treated at 100 ° C for 1 minute to 60 minutes, the content of Rg ₂ , Rg ₃ and Rg ₅ as useful ginsenoside components It is possible to produce a high-western-style ginseng preparation.

In addition, the manufacturing method of the present invention may further include a post-processing step. The processing may be performed by a known method to prepare a processed ginseng extract. That is, it is preferable that the western triplet is extracted by using water, a lower alcohol such as methanol or ethanol, a lower ketone such as acetone or methyl ethyl ketone, a supercritical fluid or a mixed solvent thereof, The solution is dried to remove the solvent, and the dried powdery processed ginseng extract, i.e., ginseng, can be prepared in the form of soft or dry extract.

In another embodiment of the present invention, there is provided a process for producing a prosapogenin composition, which comprises preparing a prosapogenin component which is produced by the above-described production method and comprises ginsenoside ≪ / RTI >

Here, the ginsenosides are ginsenosides Rb ₁ , Rb ₂ , Rc, Rd, Re, Rf, Rg 1, Rg 2, Rg 3, Rg 5, Rg 6, Rh 1, Rh 4, Rk 1, Rk 3, F 1 and binary respect to the sum of F ₄ ginsenoside Rg _2, Rg ₃ , Rg ₅ And it is _preferably a ₁ Rk contains a high concentration in a 1% by weight to 60% by weight.

In particular, it is contained at a high concentration of 0.1 to 10.0% of Rg _{3 as a} functional substance, 0.1 to 4.0% of Rg ₅ , 0.1 to 3.0% of Rk _{1 and} 0.1 to 3.0% of Rg ₂ ,

That is, the Western blotting agent according to the present invention, which is manufactured by the above method and contains ginseng prosapogenin at a high concentration, Improvement of metabolic syndrome, anticancer, blood pressure lowering, sexual function improvement, liver function improvement, brain cognitive function improvement, ADHD improvement, atopy and allergy improvement.

Furthermore, the total daily dose to be administered to the host at a single dose or two to three separate doses for clinical use of the western blods of the present invention is preferably in the range of 1 to 50 mg / kg of body weight, The specific dose level for a particular compound may vary depending on the particular compound being employed, body weight, age, sex, health condition, diet, time of administration, method of administration, excretion rate, drug mix and severity of the disease.

The compounds of the present invention may be administered as injectable preparations and oral preparations as desired. Injectable preparations, for example sterile injectable aqueous or oleaginous suspensions, may be prepared according to the known art using suitable dispersing agents, wetting agents or suspending agents. Solvents that may be used herein include water, Ringer's solution and isotonic NaCl solution, and sterile, fixed oils are typically used as a solvent or suspending medium. Any non-irritating fixed oils, including mono-, di-glycerides, may be used for this purpose, and fatty acids such as oleic acid may be used in injectable preparations. Dosage forms for oral administration include capsules, tablets, pills, powders, granules and solutions, and particularly capsules, tablets and liquids are useful. Tablets and pills are preferably prepared as preservative. Solid and liquid dosage forms can be prepared by mixing the active ingredient with one or more inert diluents such as sucrose, lactose, starch and the like, lubricants such as magnesium stearate, talc, disintegration aids such as calcium CM, binders, fragrances, preservatives such as sodium benzoate , Sweeteners such as sugars or fructose, and surfactants. More specifically, a capsule may be prepared by adding starch and lactose as an excipient at a ratio of 7: 3 as an excipient, and adding magnesium stearate and talc at 3% or less for increasing fluidity. The starch and lactose Is added at a ratio of 7: 3, followed by tableting with calcium carbonate as a binder and disintegration aid. Flavor as a fragrance, sodium benzoate as a preservative, sugar or fructose as a sweetener, and a surfactant are added to a liquid Can be produced.

The present invention may be better understood by the following examples, which are for the purpose of illustrating the invention and are not intended to limit the scope of protection defined by the appended claims.

Manufacturing example  : Western ginseng  Manufacture of X

2,500 ml of ethyl alcohol was added to 500 g of each of the dried products of white ginseng (white ginseng, red ginseng, and minced ginseng), and the mixture was refluxed four times, filtered and concentrated under reduced pressure to obtain ethyl alcohol western ginseng extract (western ginseng ethanol extract).

Example  1 ~ 7: Using ultrasonic Western ginseng  Manufacture of pharmaceutical preparations

Example  1: Ultrasonic treatment for 5 minutes with distilled water, Western ginseng  Manufacture of pharmaceutical preparations

200 ml of distilled water was added to 1 g of western ginseng ethanol extract obtained from the above Preparation Example, and this was placed in a sonicator of 600 W, 600 W, 100 ° C, and sonication for 5 minutes. Then, this was repeatedly extracted twice with 95% ethyl alcohol for 2 hours, concentrated under reduced pressure, and lyophilized to obtain a brownish extract.

Example  2: Ultrasonic treatment with distilled water for 10 minutes, Western ginseng  Manufacture of pharmaceutical preparations

An extract was obtained by the method of Example 1, except that the ultrasonic treatment was performed for 10 minutes in the above Example 1.

Example  3: Ultrasonic treatment with distilled water for 20 minutes, Western ginseng  Manufacture of pharmaceutical preparations

The extract was obtained by the method of Example 1, except that the ultrasonic treatment was carried out for 20 minutes in the above Example 1.

Example  4: Ultrasonic treatment with distilled water for 30 minutes, Western ginseng  Manufacture of pharmaceutical preparations

An extract was obtained by the method of Example 1, except that the ultrasonic treatment was performed for 30 minutes in the above-mentioned Example 1.

Example  5: Ultrasonic treatment with distilled water for 40 minutes, Western ginseng  Manufacture of pharmaceutical preparations

An extract was obtained by the method of Example 1, except that the ultrasonic treatment was performed for 40 minutes in Example 1.

Example  6: Ultrasonic treatment with distilled water for 60 minutes, Western ginseng  Manufacture of pharmaceutical preparations

An extract was obtained by the method of Example 1, except that the ultrasonic treatment was performed for 60 minutes in Example 1.

Example  7: Ultrasonic treatment with vinegar for 40 minutes, Western ginseng  Manufacture of pharmaceutical preparations

An extract was obtained in the same manner as in Example 1, except that the vinegar was used in place of the distilled water in Example 1 and ultrasonicated for 40 minutes.

Comparative Example  1: without ultrasonic treatment Western ginseng  Manufacture of pharmaceutical preparations

To 500 g of western gypsum was added 2,500 ml of ethyl alcohol, and the mixture was refluxed twice, filtered, and concentrated under reduced pressure to obtain ethyl alcohol. Then, the obtained ethyl alcohol wax extract was lyophilized to obtain a brownish extract.

Experimental Example  : HPLC Through law Gin Senocide  Component analysis

① Experimental method

50 ml of diethylether was added to each 2 g of each of the western ginseng extract preparations obtained in Examples 1 to 7 and Comparative Example 1, and the mixture was centrifuged three times for 1 hour, and the supernatant was removed.

Subsequently, 50 ml of butanol saturated with water was added to the obtained residue, and the mixture was extracted three times for 2 hours. The resulting mixture was centrifuged, and the supernatant was filtered and concentrated under reduced pressure to obtain crude saponin. The thus obtained crude saponin was quantified by HPLC method, and the results are shown in Tables 1 to 3 below.

② HPLC analysis conditions

The results obtained by the above-mentioned experimental method were directly compared with the specimens. That is, the content and the composition of ginseng saponin were repeatedly tested three times per each sample to confirm the reproducibility of the results. The product used was ginsenoside of 99% or more purity purchased from Chromadex (U.S.A.).

The HPLC apparatus used was a Waters 1525 binary HPLC system (Waters, USA) and a column was Eurospher 100-5 C18 (250 * 3 mm).

The mobile phase was acetonitrile (HPLC grade, Sigma, USA) and distilled water for HPLC. The ratio of acetonitrile was changed from 17% (0 min) to 30% (55 min), 40% (80 min), 60% % (140 min) and finally adjusted to 17%. The development temperature was measured at room temperature, the flow rate was 0.8 ml per minute, and the chromatogram was detected at 203 nm using uv / vis Waters 2487 Dual λ Absorbance Detector (Waters, U.S.A.) detector.

As described above, the sample was directly compared with the sample through HPLC, and the average was statistically calculated.

Table 1 below shows the ginsenoside content of the western ginseng extract obtained by the above Examples 1 to 7 and Comparative Example 1.

As shown in Table 1, analysis of ginseng saponin ginsenosides Rb _1, Rb _2, Rc, Rd, Re, Rf, Rg _1, Rg _2, Rg _3, Rg _5, Rg _6, Rh _1, Rh ₄ , Rk _1, Rk _3, F _1, F ₄ was, embodiments of the present invention 1 to example 7 useful pro sapogenin component (ginsenoside Rg _2, Rg _3, Rg _{5 and,} Rk ₁₎ is highly confirmed .

In particular, when Western ginseng was treated with distilled water for 40 minutes (Example 5), ginsenoside Rg ₃ was 10.698%, ginsenoside Rg ₅ Was found to be high in the concentration of 4.525%, and it was confirmed that the ginsenoside Rg ₂ Was found to be 2.998% in 30 minutes of ultrasound treatment, while ginsenoside Rk ₁ showed a high concentration of 3.456% in 30 minutes of ultrasound treatment.

While the present invention has been particularly shown and described with reference to preferred embodiments thereof, it is to be understood that the invention is not limited to the disclosed embodiments, but, on the contrary, It will be apparent to those skilled in the art that changes may be made.

Claims (11)

1 part by weight of western ginseng or western ginseng extract is put into 5 to 300 parts by weight of distilled water and ultrasonicated at a temperature within a range of 90 ° C to 110 ° C for a time within a range of 30 minutes to 40 minutes; And
And concentrating the ultrasound-treated western ginseng or western ginseng extract under reduced pressure and lyophilization,
Wherein the content of ginsenosides Rg ₂ , Rg ₃ and Rg ₅ is increased.
delete delete The method according to claim 1,
Wherein the ultrasound treatment is performed using an ultrasonic generator of 600W.
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