KR100706111B1 - A ginseng preparation using vitamin c and process for thereof - Google Patents

Abstract

The present invention relates to a ginseng preparation using vitamin C (Ascorbic acid) and a method for preparing the same, and more specifically, made by dissolving vitamin C in distilled water at a predetermined ratio (vitamin C 10 g / distilled water 50 ml to 100 ml) in ginseng. Red ginseng was prepared by adding a vitamin C solution and heating it for 1 to 10 hours to prepare a ginseng preparation containing high concentrations of ginsenosides Rg _{2 ,} Rg _{3 ,} Rh ₁ , and Rh ₂ as ginseng prosapogenin. relates a small amount of red ginseng specific component produced in ginsenoside _{Rg 2, Rg 3, Rh 1} , Rh 2 by the heat when the ginseng formulations and methods for their preparation containing a high concentration.

Description

Ginseng preparation using vitamin C and preparation method thereof

The present invention relates to a method for preparing ginseng preparations using vitamin C (Ascorbic acid), and more specifically, ginsenoside (ginsenoside) Rg _2, Rg by adding a distilled water solution of vitamin C to ginseng and heat-extracting for 1 to 10 hours. _{The present} invention relates to a method for producing a ginseng preparation containing a high concentration of Rh ₂ for preparing a ginseng preparation containing a high concentration of _3, Rh ₁ and Rh ₂ .

Korean ginseng ( Panax ginseng ) is a summary of the original energy of the human body's vitality, which is described in the product of the new agricultural plant , the best herbal book of the East. Korean ginseng is a representative physiologically active substance, ginsenoside, which is a triterpenoid ginseng saponins, polyacetylene having an anticancer activity, a phenolic compound having an antioxidant action, and a radiation protective action. It contains ginseng proteins, acidic polysaccharides with immunomodulatory activity, and the like. In particular, Korean ginseng contains a large amount of phenolic compounds, polyacetylene, and acidic polysaccharide components compared to Western ginseng ( Panax quinquefolium ), it is expected that better physiological activity. Ginsenoside, a ginseng saponin, known as a major pharmacological component of Korean ginseng, has been identified by its Shibata group at Tokyo University. The ginseng saponins contained in Korean ginseng are about 30 species and contain much more ginseng saponins than 14 kinds of Western ginseng and 15 kinds of Panax notoginseng . Gene major component of ginsenoside Rb ₁ of ginsenosides in the protocol wave incident diol group (ginsenoside Rb _1, Rb _2, Rc, Rd, Rg ₃₎ represents the inhibitory central nervous, protocol wave incident triol group (ginsenoside Re Ginsenoside Rg ₁ , a major component of Rf, Rg ₁ , Rg ₂ , and Rh ₁ ), is known to exhibit central nervous system excitability and contribute to the adaptogen activity of Korean ginseng.

In Korean ginseng, the content ratio (diol / triol) of the ProtoPanacodiol group and ProtoPanacotri triol group and the content ratio (Rb ₁ / Rg ₁ ) of Ginsenoside Rb ₁ and Ginsenoside Rg ₁ were 1.96 and 3.14, which is more balanced than the western ginseng's 2.48 and 25.96, which exhibits excessive central nervous system suppression, but exhibits a true viewing rather than excitement. Therefore, Korean ginseng is a herbal pharmacological effect, which has a balanced and stable dexterity effect between the ginsenoside Rb ₁ and the ginsenoside Rg _{1, which} are both stable and stable. It is considered to be the best tonic of modern people.

The pharmacological activities of Korean ginseng identified to date include reinforcement of heart and blood vessels, recovery of blood function, improvement of blood flow, prevention of arteriosclerosis and hypertension, enhancement of gastrointestinal regulation, promotion of liver function and hangover, anti-fatigue and anti Stress action, anti-aging action, brain activity promoting action, anti-inflammatory activity, allergic disease treatment, gynecological disease, diabetes mellitus, anti-radiation action, energy enhancement, anti-tumor action, improvement of learning ability, inhibition of production of lipid oxide, promotion of wound treatment, Improving immune capacity, inhibiting AIDS virus proliferation, and promoting protein synthesis have been reported.

In addition, red ginseng extracted from fresh ginseng from the field is removed, and red ginseng is dried naturally in daylight and white ginseng is dried.

In particular, red ginseng is produced by heat, and the small amount of ginsenosides such as Rg ₂ , Rg _3, Rh ₁ , and Rh ₂ has the advantages of red ginseng because it has cancer prevention, cancer metastasis suppression, blood pressure lowering and antioxidant activity. It is attracting attention.

As mentioned above, Korean ginseng has been recognized as the best brand for a long time, but currently occupies about 3% market share in the global ginseng market. To innovate the ginseng industry, it is necessary to develop high value-added ginseng products. Do. Nevertheless, ginseng preparations and red ginseng preparations up to now have been developed at the level of tonics, with a simple extract level preparation. However, in order to develop more professional and functional ginseng preparations, it will be necessary to develop a ginseng preparation containing a high concentration of functional substances with strong physiological activity and safety.

For this reason, in recent years, methods for making ginseng preparations containing a large amount of ginsenoside Rg ₃ having excellent effects among ginseng components have been tried.

According to the Shibata paper published in 1966, hydrolysis of saponins with weak acids such as acetic acid hydrolyzed only C-20 glucoside bonds (ginsenosides Rb ₁ , Rb ₂ , Rc, Rd) and prosapogenin (20). (R & S) -ginsenoside Rg ₃ ) has been published, but it has only been made into a standard. In addition, other researchers have used a method of ginsenoside Rg ₃ containing a large amount of enzyme treatment the physical reaction of high-temperature treatment or biochemical reaction to make a ginseng preparation.

The method of high temperature treatment is to obtain processed ginseng with enhanced efficacy by, for example, heating ginseng at a high temperature. The ginseng is heat treated at a high temperature of 120 to 180 ° C. for 0.5 to 20 hours to obtain ginsenosides (Rg ₃ + Rg _5). It is for obtaining processed ginseng products in which the active ingredient is increased so that the ratio of) / (Rc + Rd + Rb ₁ + Rb ₂ ) is 1 or more. The patent on a beverage composition containing the processed ginseng product is known from Korean Patent No. 192678. The rare anticancer saponin (Rh ₁ , Rh ₂ ) is hydrolyzed by saponin glucoside degrading enzyme to hydrolyze sugar groups of ginseng saponin. Patents related to the manufacturing method are known in Korean Patent No. 329259 and the like. Currently, commercially available products are marketed under the trade names Sunsam and Sinsam. However, this method increases the efficacy, but it takes a long time to manufacture or requires special equipment such as a high-pressure heater, and in particular, the ginseng is carbonized when mass production because it must be heated to a higher temperature than the normal processing temperature, etc. There are many difficulties in the operation of the manufacturing process.

A process for the preparation of ginsenosides Rg ₃ and / or Rg ₅ is disclosed in Korean Patent No. 228510, and in Korean Patent No. 201585 for Vascular Relaxant Compositions, under mild conditions instead of heat treatment at high temperatures, for example, dilute mines. In addition, it is described that an active ingredient such as Rg ₃ is produced even under acid treatment with a lower organic acid such as acetic acid, tartaric acid, oxalic acid, and the like under the reaction conditions of heat treatment at high temperature.

On the other hand, if you look at the history of vitamin-C, in 1497, about 60% of the crew began to die for an unknown reason during the months of Vasco da Gama's voyage to the East Indies. One of them is bleeding easily from the gums and oral mucosa. For that reason, the disease was called "scurvy."

In 1907, Norway's Dr. Holst and Frorich proved experimentally that green vegetables are very effective in developing scurvy and treating cures in guinea pigs (a kind of rat). In addition, since the body cannot synthesize this substance, I realized that if I do not eat green vegetables, I have to suffer from scurvy.

Ascorbic acid, also known as vitamin-C, is derived from the anti-scurvy factor, or anti-scorbutic acid. Dr. Warp succeeded in extracting this substance from lemon juice at the University of Pittsburgh to cure guinea pigs with scurvy, and for the first time confirmed that vitamin-C was actually extracted and that scurvy came from a lack of this substance.

In 1933, Swiss scientist Reichstein succeeded in the first biosynthesis of vitamin C, and experimentally began to open the way for the synthesis of large amounts of vitamin C. And finally, in 1938, the name ascorbic acid was officially accepted worldwide as the official chemical name for vitamin-C. In addition, the pharmacological action of vitamin C has been reported antioxidant activity and active oxygen scavenging effect, and many research results such as sunscreen effect has been published.

Products using vitamin C are available in tablets, liquids, and capsules, and simple mixed drinks with ginseng are already on the market, but vitamin C is used to induce changes in the components of ginseng to produce high concentrations of functional substances. The technology to be included may be said to be the first to be different from existing technologies and products.

Therefore, the inventors of the present invention, unlike the processing method of heating the ginseng at a high temperature during continuous research to prepare a ginseng preparation containing a high concentration of functional substances such as ginsenosides Rg ₂ , Rg _3, Rh ₁ , Rh ₂ Ginsenoside Rg ₂ , Rg _3, Rh after adding 10 g / distilled water 50 ml to 100 ml of vitamin C in distilled water at a constant ratio to ginseng extract ₁ , it was found that ginseng preparation containing a high concentration of Rh ₂ can be prepared to complete the present invention.

Therefore, the present invention is ginsenoside Rg ₂ , Rg _3, Rh ₁ , Rh ₂ is ginseng saponin glycoside ginsenoside Rb ₁ , Rb ₂ , Ratios for Rc, Rd, Re, Rf, Rg ₁ (ginsenosides Rg ₂ , Rg _3, Rh ₁ , Rh ₂ / ginsenosides Rb ₁ , Rb ₂ , It is an object of the present invention to provide a method for economically preparing a ginseng preparation containing Rc, Rd, Re, Rf, and Rg ₁ ) at a high concentration of 0.3 or more and less than 1.0.

In the present invention, a solution of vitamin C and distilled water mixed with vitamin C 10g and distilled water at a ratio of 50ml to 100ml is added to ginseng extract and heated and extracted for 1 to 10 hours to obtain ginsenosides Rg ₂ , Rg _3, Rh ₁ , and Rh. ₂ the ginsenoside Rb _1, Rb ₂ , It consists of a production method of the ginseng formulation made to be contained in a 30-100% for the total of Rc, Rd, Re, Rf, Rg 1.

Hereinafter, the present invention will be described in more detail.

The present invention relates to a method for preparing a ginseng preparation containing high concentration ginsenosides Rg ₂ , Rg _{3 ,} Rh ₁ , Rh ₂ , using vitamin C as a functional substance ginsenosides Rg ₂ , Rg _{3 ,} Rh ₁ , Rh ₂ It relates to a new concept ginseng preparation containing a high concentration.

As described above, the present invention is characterized by using vitamin C to maximize the change in the active ingredient in ginseng and the content of rare ginseng components to enhance the potency and to serve as an auxiliary role.

Referring to the production method of the ginseng preparation according to the present invention in more detail as follows.

A solution obtained by mixing 10 g of vitamin C in a ratio of 5 to 10 times the amount of distilled water is added to ginseng extract and heated and extracted at 70 to 150 ° C. for 1 to 24 hours to obtain a ginseng preparation. If the heating temperature is less than 70 ℃ to obtain a very small amount of the desired active ingredient in the final product obtained, if it exceeds 150 ℃ the content of the active ingredient is also lowered and there are many difficulties in the operation of the manufacturing process. In addition, when the heating time is less than 1 hour, a desired amount of the desired active ingredient is obtained in the final obtained product, and if it exceeds 24 hours, the desired ginsenoids Rg ₂ , Rg _3, Rh ₁ , and Rh ₂ may be decomposed in the final obtained product. And, the final extract according to the present invention may be in any form, such as liquid or powder.

In particular, ginsenosides Rg ₂ , Rg _{3 ,} Rh ₁ , Rh ₂ are added to the ginseng extract with a certain ratio (vitamin C 10 g / distilled water 50 ml to 100 ml) and extracted for 1 to 24 hours at 70-150 ° C. Ginsenoside Rb ₁ , Rb ₂ , It contains 30 to 100% of Rc, Rd, Re, Rf, and Rg ₁ and can be useful for treating hyperglycemia, metabolic syndrome, improving blood circulation, improving erectile dysfunction, fatigue recovery, hypertension, arteriosclerosis, thrombosis, and stroke .

In the present invention, which part of the ginseng is used cannot be an important factor in determining the effect of the present invention. Therefore, in the present invention, the ginseng X refers to Korean ginseng ( Panax Panax , including ginseng quinquefolium), three hundred seventy-three (Panax notoginseng), jukjeol three (Panax japonicum ) or an extract of the above-ground or underground herbal medicines of plants of the genus Panax , such as Panax vietnamensis . It includes one case, which is based on the findings from repeated experiments involving ginseng.

At this time, the processing may be extracted by a known method if necessary to make a processed ginseng extract. That is, after extracting ginseng using water, lower alcohol (e.g. methanol, ethanol, etc.), lower ketone (e.g. acetone, methyl ethyl ketone, etc.), supercritical fluid, or a mixed solvent thereof, the concentrated or concentrated liquid The dried ginseng extract, ie, ginseng, may be prepared in the form of a soft or dried extract by drying the dried powdery processed ginseng extract.

In addition, the vitamin C used by this invention can use both the thing obtained from a natural product and the thing obtained by organic synthesis as a lascorobic acid.

The present invention ginsenosides Rg ₂ , Rg _3, Rh ₁ , Rh ₂ prepared by the same method as ginsenosides Rb ₁ , Rb ₂ , It contains 30 to 100% of Rc, Rd, Re, Rf, and Rg ₁ so that these ginseng preparations can treat hyperglycemia, metabolic syndrome, improve blood circulation, improve erectile dysfunction, recover from fatigue, hypertension, arteriosclerosis, antithrombosis, and stroke And preventive and brain function improvement purposes.

Ginseng preparation prepared according to the method of the present invention as described above ginsenoside Rg₂, Rg_3,Rh_One, Rh₂Ginsenoside Rb_One, Rb₂, Rc, Rd, Re, Rf, Rg_OneIt contains about 30-100% of high concentration, and it is excellent in efficacy.

When the ginseng preparation of the present invention is administered for clinical purposes, the total daily dose to be administered to the host in a single dose or in two or three separate doses is preferably in the range of 1 to 50 mg / kg body weight, but specific for a particular patient. Dosage levels may vary depending on the specific compound to be used, weight, age, sex, health condition, diet, time of administration, method of administration, rate of excretion, drug mixture and severity of the disease.

The compounds of the present invention can be administered in injectable and oral formulations as desired. Injectable preparations, for example sterile injectable aqueous or oleaginous suspensions, can be prepared using suitable dispersing agents, wetting agents or suspending agents according to known techniques. Solvents that can be used include water, Ringer's solution and isotonic NaCl solution, and sterile fixed oils are conventionally employed as a solvent or suspending medium. Any non-irritating fixed oil, including mono-, diglycerides can be used for this purpose, and fatty acids such as oleic acid can be used in the preparation of injectables. Dosage forms for oral administration may be capsules, tablets, pills, powders, granules, and solutions. Particularly, capsules, tablets, and solutions are useful. Tablets and pills are preferably prepared with enteric agents. Solid and liquid dosage forms contain the active ingredient in one or more inert diluents such as sucrose, lactose, starch, etc., lubricants such as magnesium stearate, talc, disintegrating aids such as calcium CMC, preservatives such as binders, fragrances, sodium benzoate And a sweetener such as sugar or fructose and a carrier selected from surfactants. Excipients other than the active ingredient may be prepared by adding starch and lactose in a ratio of 7: 3, and adding magnesium stearate and talc to within 3% to increase fluidity, and a formulation containing these ingredients may be prepared as a capsule. As an excipient, starch and lactose may be added in a ratio of 7: 3, and a binder and a disintegration aid may be added to calcium CMC and compressed into tablets.As a flavoring agent, a fruit flavor, sodium benzoate as a preservative, sugar or fructose and an interface An active agent may also be added to prepare a liquid formulation.

Hereinafter, the present invention will be described in more detail with reference to the following Examples and Experimental Examples. However, these examples and comparative examples are only to aid the understanding of the present invention, and are not intended to limit the scope of the present invention in any way.

Comparative example  One : Ginseng extract  Produce

500 g of dried ginseng and 250 ml of 95% ethanol (alcohol) were added to an airtight container, followed by shaking for 4 hours at 76 ° C. in a water bath to extract and filter dried ginseng x four times to prepare ginseng x.

Comparative example  2 : Red ginseng extract  Produce

500 g of red ginseng and 250 ml of 95% ethanol (alcohol) were added to an airtight container, and red ginseng X was prepared by vacuum drying the red ginseng X obtained by extracting and filtering four times by stirring for 4 hours in a 76 ° C water bath.

Example  One

Vitamin C solution (10 g of vitamin C was dissolved in 50 ml of distilled water) was added to 5 g of ginseng x 5 g and extracted once at 100 ° C. for 1 hour. The filtrate was lyophilized to give a brown extract, and ginseng saponin was analyzed by HPLC method.

Example  2

Vitamin C solution (10 g of vitamin C was dissolved in 60 ml of distilled water) was added to 5 g of ginseng x 5 g, and extracted once at 100 ° C. for 1 hour. The filtrate was lyophilized to give a brown extract, and ginseng saponin was analyzed by HPLC method.

Example  3

Vitamin C solution (10 g of vitamin C was dissolved in 70 ml of distilled water) was added to 5 g of ginseng x 5 g, and extracted once at 100 ° C. for 1 hour. The filtrate was lyophilized to give a brown extract, and ginseng saponin was analyzed by HPLC method.

Example  4

Vitamin C solution (10 g of vitamin C was dissolved in 80 ml of distilled water) was added to 5 g of ginseng x 5 g and extracted once at 100 ° C. for 1 hour. The filtrate was lyophilized to give a brown extract, and ginseng saponin was analyzed by HPLC method.

Example  5

Vitamin C solution (10 g of vitamin C was dissolved in 90 ml of distilled water) was added to 5 g of ginseng x 5 g and extracted once at 100 ° C. for 1 hour. The filtrate was lyophilized to give a brown extract, and ginseng saponin was analyzed by HPLC method.

Example  6

Vitamin C solution (10 g of vitamin C was dissolved in 50 ml of distilled water) was added to 5 g of ginseng x 5 g, and extracted once at 100 ° C. for 5 hours. The filtrate was lyophilized to give a brown extract, and ginseng saponin was analyzed by HPLC method.

Example  7

Vitamin C solution (10 g of vitamin C was dissolved in 60 ml of distilled water) was added to 5 g of ginseng x 5 g, and extracted once at 100 ° C. for 5 hours. The filtrate was lyophilized to give a brown extract, and ginseng saponin was analyzed by HPLC method.

Example  8

Vitamin C solution (10 g of vitamin C was dissolved in 70 ml of distilled water) was added to 5 g of ginseng x 5 g, and extracted once at 100 ° C. for 5 hours. The filtrate was lyophilized to give a brown extract, and ginseng saponin was analyzed by HPLC method.

Example  9

Vitamin C liquid (10 g of vitamin C was dissolved in 80 ml of distilled water) was added to 5 g of ginseng x 5 g, and extracted once at 100 ° C. for 5 hours. The filtrate was lyophilized to give a brown extract, and ginseng saponin was analyzed by HPLC method.

Example  10

Vitamin C liquid (10 g of vitamin C was dissolved in 90 ml of distilled water) was added to 5 g of ginseng x 5 g, and extracted once at 100 ° C. for 5 hours. The filtrate was lyophilized to give a brown extract, and ginseng saponin was analyzed by HPLC method.

Instead of the ginseng X used in Examples 1 to 2 may be applied by selecting the X of the red ginseng, Taegeuksam, ginseng leaf, ginseng fruit.

Example  11

Vitamin C solution (10 g of vitamin C was dissolved in 50 ml of distilled water) was added to 5 g of red ginseng x 5 g, and extracted once at 100 ° C. for 1 hour. The filtrate was lyophilized to give a brown extract, and ginseng saponin was analyzed by HPLC method.

Example  12

Vitamin C solution (10 g of vitamin C was dissolved in 50 ml of distilled water) was added to 5 g of red ginseng x 5 g, and extracted once at 100 ° C. for 2 hours. The filtrate was lyophilized to give a brown extract, and ginseng saponin was analyzed by HPLC method.

Example  13

Four times of vitamin C solution (10 g of vitamin C was dissolved in 50 ml of distilled water) was added to 5 g of red ginseng extract and extracted once at 100 ° C. for 3 hours. The filtrate was lyophilized to give a brown extract, and ginseng saponin was analyzed by HPLC method.

Example  14

Four times of vitamin C liquid (10 g of vitamin C was dissolved in 50 ml of distilled water) was added to 5 g of red ginseng extract and extracted once at 100 ° C for 4 hours. The filtrate was lyophilized to give a brown extract, and ginseng saponin was analyzed by HPLC method.

Example  15

Four times of vitamin C solution (10 g of vitamin C was dissolved in 50 ml of distilled water) was added to 5 g of red ginseng extract and extracted once at 100 ° C. for 5 hours. The filtrate was lyophilized to give a brown extract, and ginseng saponin was analyzed by HPLC method.

Example  16

Four times of vitamin C solution (10 g of vitamin C was dissolved in 50 ml of distilled water) was added to 5 g of red ginseng extract and extracted once at 100 ° C. for 6 hours. The filtrate was lyophilized to give a brown extract, and ginseng saponin was analyzed by HPLC method.

Example  17

Four times of vitamin C solution (10 g of vitamin C was dissolved in 50 ml of distilled water) was added to 5 g of red ginseng extract and extracted once at 100 ° C. for 7 hours. The filtrate was lyophilized to give a brown extract, and ginseng saponin was analyzed by HPLC method.

Example  18

Four times of vitamin C liquid (10 g of vitamin C was dissolved in 50 ml of distilled water) was added to 5 g of red ginseng extract and extracted once at 100 ° C for 8 hours. The filtrate was lyophilized to give a brown extract, and ginseng saponin was analyzed by HPLC method.

Example  19

Vitamin C solution (10 g of vitamin C was dissolved in 50 ml of distilled water) was added to 5 g of red ginseng x 5 g, and extracted once at 100 ° C. for 9 hours. The filtrate was lyophilized to give a brown extract, and ginseng saponin was analyzed by HPLC method.

Example  20

Vitamin C solution (10 g of vitamin C was dissolved in 50 ml of distilled water) was added to 5 g of red ginseng x 5 g, and extracted once at 100 ° C. for 10 hours. The filtrate was lyophilized to give a brown extract, and ginseng saponin was analyzed by HPLC method.

Experimental Example  One: HPLC Through the law Ginsenoside  Ingredient analysis

① Experiment Method

1g each of the samples produced in Comparative Example 1 and Examples 1 to 16 was dissolved in 100 ml of methanol and filtered, and quantified as shown in Figure 1 by HPLC method, and the results are shown in Table 1 and FIG. 1.

② HPLC analysis conditions

Each ginseng saponin component was prepared based on 1 mg / mL (1000 ppm) calibration curve, where HPLC conditions were as follows:

      HPLC: Alltel Binary Gradient HPLC System (Model 627)

Column: Pre-Vale Carbohydrate YES Column

Detector: Alltech ELSD 2000 YES Detector

Temperature: room temperature

Flow rate: 0.8 mg / ml

Mobile phase: Solvent A-ACN: Water: IPA = 80: 5: 15

               Solvent B-ACN: Water: IPA = 67: 21: 12

       ingredient

 Time  0  28  35  45  50  51  57  58  65  % B  10  85  80  75  90  100  25  10  10

[ standard ]

[ Example 15 ]

Ginsenoside Content of Ginseng Preparation Ginsenoside Comparative Example 1 Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Example 7 Example 8 Example 9 Example 10 Rh2 0 0.439 0 0 0 0 0.782 0 0 0 0 Rh1 0 0.096 0 0 0 0 0.327 0.192 0.232 0 0 Rg2 0 0.166 0.072 0.113 0.100 0.078 0.254 0.178 0.153 0.127 0.131 Rg3 0 0.595 0.035 0.153 0.106 0.078 2.683 0.424 0.268 0.125 0.164 Rg1 0.318 0.329 0.111 0.179 0.210 0.234 0.616 0.234 0.123 0.226 0.328 Rf 0.439 0.147 0.229 0.413 0.253 0.356 0.289 0.260 0.236 0.349 0.313 Re 1.982 0.345 1.218 1.494 1.639 1.844 0.553 3.621 1.096 1.304 1.695 Rd 1.707 1.123 2.591 2.551 2.594 2.276 1.491 1.706 1.599 1.873 2.083 Rc + Rb2 2.081 0.647 1.381 1.787 2.066 2.194 0.547 1.815 2.090 1.759 2.312 Rb1 4.613 1.614 3.150 3.956 4.535 4.668 1.257 3.811 4.079 4.296 5.413 * 0 30.82 1.23 2.56 1.82 1.35 85.13 6.94 7.08 2.57 2.43

*: Ginsenosides Rg ₂ , Rg _{3 ,} Rh ₁ , Rh ₂ / ginsenosides Rb ₁ , Rb ₂ , Rc, Rd, Re, Rf, Rg ₁ × 100

As shown in Table 1, as a result of analyzing the content of various ginsenosides by the HPLC method from the ginseng extract (preparation formulation) and the ginseng formulation (example formulation) obtained according to the method of the present invention, red ginseng specific to ginseng extract Ginsenosides Rg ₂ , Rg _{3 ,} Rh ₁ , and Rh ₂ were detected in the ginseng preparation of the present invention, whereas the components ginsenosides Rg ₂ , Rg _3, Rh ₁ , and Rh ₂ were not detected at all. . In particular, the ginseng prosapogenin ginsenosides Rg ₂ , Rg _{3 ,} Rh ₁ , Rh ₂ content of 4.046% of the ginseng saponin of Example 6 preparations treated with vitamin C solution of vitamin C 10g / 50 ml of distilled water for 5 hours. Glycosides (ginsenoside Rb ₁ , Rb ₂ , Rc, Rd, Re, Rf, Rg ₁ ) content of 85.13% in the high content ratio, ginsenoside Rg also in the case of Example 1 formulation treated with vitamin C solution of vitamin C 10g / 50ml of distilled water for 1 hour The content of ₂ , Rg _{3 ,} Rh ₁ , Rh ₂ was 1.296%, indicating a high content ratio of 30.82% in ginseng saponin glycosides. Therefore, it is thought that the extraction ratio of vitamin C solution dissolved in 50 ml of distilled water in 10 g of vitamin C can be used to prepare a high concentration of ginseng prosapogenin ginseng preparations. Was reviewed.

Ginsenoside Comparative example  2 Vitamin C Liquid (Vitamin C 10g / 50ml of Distilled Water) room Example 11 room Example 12 room Example 13 room Example 14 room Example 15 room Example 16 room Example 17 room Example 18 room Example 19 room Example 20 Rh2 0.257 0.439 0.582 0.524 0.670 0.774 0.782 0.689 0.732 0.749 0.778 Rh1 0.277 0.087 0.092 0.106 0.127 0.222 0.268 0.273 0.262 0.256 0.271 Rg2 0.101 0.211 0.198 0.226 0.225 0.257 0.278 0.263 0.278 0.253 0.252 Rg3 0.306 0.853 0.787 1.539 1.782 2.795 1.700 2.843 2.948 3.390 3.434 Rg1 0.282 0.353 0.386 0.515 0.569 0.703 0.591 0.682 0.688 0.701 0.716 Rf 0.256 0.252 0.253 0.278 0.252 0.305 0.342 0.367 0.359 0.370 0.373 Re 2.815 1.192 1.141 1.056 1.057 1.159 1.056 1.001 0.861 0.971 1.003 Rd 1.323 0.592 0.613 0.528 0.542 0.790 1.095 0.746 1.042 0.857 0.946 Rc + Rb2 2.034 0.940 1.028 0.678 0.617 0.533 0.839 0.636 0.651 0.623 0.547 Rb1 3.936 1.687 1.778 1.182 1.072 0.939 1.449 3.546 3.194 3.001 2.578 * 0.088 31.70 31.91 56.53 68.24 91.40 56.37 58.30 62.11 71.26 76.83

*: Ginsenosides Rg ₂ , Rg _{3 ,} Rh ₁ , Rh ₂ / ginsenosides Rb ₁ , Rb ₂ , Rc, Rd, Re, Rf, Rg ₁ × 100

In the results of Table 2, when treating the vitamin C solution of vitamin C 10g / 50ml of distilled water using red ginseng extract, and reviewed by time zone, as in Example 15, ginsenoside Rg ₂ , Rg _{3 ,} Rh ₁ , Rh ₂ was 91.40% of ginseng saponin glycosides, and it was confirmed that ginseng prosapogenin was produced at the highest concentration than other time periods. However, a high concentration of ginseng prosapogenin at 50% or higher from 3 hours extraction to 10 hours extraction was possible.

Therefore, according to the present invention, the concentration of vitamin C (vitamin C 10g / distilled water 50-100ml) and the extraction time (1-10 hours) are appropriately adjusted under the reaction temperature conditions of 1-24 hours at 70-150 ° C. It was confirmed that ginseng preparations containing high concentrations of Rg ₂ , Rg _{3 ,} Rh ₁ , and Rh ₂ could be prepared.

As described above, the present invention can easily prepare a ginseng preparation containing a high concentration of ginsenosides Rg ₂ , Rg _{3 ,} Rh ₁ , Rh ₂ , which are functional substances produced in a small amount by heat when preparing red ginseng.

Claims (11)

Ginsenosides Rg ₂ , Rg _{3 ,} Rh ₁ , and Rh ₂ are ginsenosides Rb ₁ , added with vitamin C solution (vitamin C 10g / distilled water 50-100ml) to ginseng or ginseng extract. Rb ₂ , A method for producing a ginseng preparation, characterized in that it is prepared to contain 30 to 100% of the total of Rc, Rd, Re, Rf, and Rg ₁ . According to claim 1, wherein the ginseng formulation is ginsenoside Rg ₃ 0.79 to 3.43%, Rg ₂ 0.20 to 0.28%, Rh ₁ 0.09 to 0.27%, Rh ₂ 0.44 to 0.78% containing ginseng preparation method characterized in that it contains. The method according to claim 1, wherein the heating temperature is 70 ~ 150 ℃. The method according to claim 1, wherein the ginseng is an underground or ground herbal medicine or extract thereof of the plant of the genus Ginseng. According to claim 4, Ginseng genus plants are selected from the group consisting of Panax ginseng , Panax quinquefolium , Panax notoginseng , Panax japonicum and Panax vietnamensis . Ginseng preparation method characterized in that The method of claim 4, wherein the ground or ground herbal medicine of the genus Ginseng is selected from the group consisting of rice, white ginseng, red ginseng, ginseng, taegeuk ginseng, ginseng leaf and ginseng fruit. delete 2. The method of claim 1, wherein the vitamin C is a natural and organic synthetic product. The method for preparing a ginseng preparation according to claim 1, wherein a vitamin C solution (vitamin C 10 g / distilled water 50 ml) is added to ginseng and ginseng extract for 3 to 10 hours at 90 to 120 ° C. The method according to claim 1, wherein ginsenosides Rg ₂ , Rg _{3 ,} Rh ₁ , Rh ₂ are ginsenosides Rb ₁ , Rb ₂ , A method for producing a ginseng preparation, characterized in that it is prepared to contain 50 to 95% of the total of Rc, Rd, Re, Rf, and Rg ₁ . Prepared by the method according to claims 1 to 10 described above, ginsenosides Rg ₂ , Rg _3, Rh ₁ , Rh ₂ are ginsenosides Rb ₁ , Rb ₂ , Process for producing a formulation containing ginseng to 30~100% of the sum of Rc, Rd, Re, Rf, Rg 1.