CN113455656A

CN113455656A - Emulsified composition

Info

Publication number: CN113455656A
Application number: CN202110338259.7A
Authority: CN
Inventors: 金秀焕; 罗相列; 郑炫禹; 金完起; 金志成; 朴赞雄
Original assignee: Amorepacific Corp
Current assignee: Amorepacific Corp
Priority date: 2020-03-31
Filing date: 2021-03-30
Publication date: 2021-10-01
Also published as: JP2021159078A; US11717012B2; US20210298339A1; KR20210121892A

Abstract

The present disclosure relates to an emulsified composition comprising ginseng seed oil and ginseng seed extract, the composition being a single phase. In the case of the composition containing ginseng seed oil as a main component, which is a conventional water-soluble component, there is a problem in that the ginseng seed oil as an oil and fat component is separated from the water-soluble component, thereby lowering the stability of the dosage form, but the emulsion composition according to the present disclosure has excellent stability because the ginseng seed oil as an oil and fat component and the water-soluble component are uniformly mixed, and thus the ginseng seed oil and the water-soluble component are not separated for a long time.

Description

Emulsified composition

Technical Field

The present disclosure relates to an emulsified composition containing ginseng seed oil and ginseng seed extract.

Background

Ginseng belongs to the genus panax of the family araliaceae, and has been used as a crude drug for the purpose of preventing diseases and prolonging life in korea, china, japan, and the like since more than 2000 years ago. The hitherto known effects and effects of ginseng are an effect on the central nervous system, an anticancer effect and anticancer activity, an immune function regulating effect, an anti-diabetic effect, a liver function hyperfunction effect, an improvement in cardiovascular disorders and an anti-arteriosclerosis effect, a blood pressure regulating effect, an improvement in climacteric disorders and an effect affecting osteoporosis, an anti-stress and anti-fatigue effect, an antioxidant activity, an anti-aging effect and the like. Ginsenosides, which are representative physiologically active ingredients of ginseng, are uniformly distributed in the above-ground and underground parts of ginseng, and a considerable amount of ginsenosides is also distributed in ginseng seeds.

Many studies have been made on the main components and pharmacological actions of ginseng, and it is expected that various adult diseases can be prevented and the possibility of onset can be reduced when ginseng is taken for a long time in consideration of the pharmacological effects of ginseng.

However, in the case of ginseng seed oil or a composition containing ginseng seed oil as a main component, it is difficult to take the composition because of the greasy feeling of oil, and therefore, it can be taken only as a capsule dosage form. In addition, when ginseng seed oil is contained as a main component containing a water-soluble component, there is a problem that ginseng seed oil as an oil component is separated from the water-soluble component, and the stability of the product is lowered.

Disclosure of Invention

In one aspect, the present invention provides a stable emulsified composition in which ginseng seed oil as an oil and fat component is uniformly mixed with a water-soluble component.

In one aspect, the present invention provides an emulsified composition comprising ginseng seed oil and ginseng seed extract.

In an exemplary embodiment, the emulsified composition is a single phase.

In an exemplary embodiment, it may be that the ginseng includes one or more selected from the group consisting of Korean ginseng (Panax ginseng), American ginseng (Panax quinquefolia), Panax notoginseng (Panax notogeng), Vietnamese ginseng (Panax vietnamensis), Panax japonicus (Panax japonica), Korean ginseng (Panax elegator), Panax quinquefolius (Panax wangianus), Panax pinnatifida (Panax bipinnatidifus) and pseudoginseng (Panax pseudoginseng).

In an exemplary embodiment, the ginseng seed extract may be water, C₁-C₆Ethanol or a mixed solvent thereof.

In an exemplary embodiment, the composition may include 0.001 wt% to 70 wt% of the ginseng seed oil, relative to the total weight of the composition.

In an exemplary embodiment, the composition may include 0.01 wt% to 60 wt% of the ginseng seed extract, relative to the total weight of the composition.

In an exemplary embodiment, the ginseng seed oil and the ginseng seed extract may be in a weight ratio of 1:2 to 1: 100.

In an exemplary embodiment, the composition may contain viscose and dietary fiber.

In an exemplary one embodiment, it may be that the viscose includes one or more selected from the group consisting of Xanthan gum (Xanthan gum), carrageenan (carageenans gum), Guar gum (Guar gum), Gellan gum (Gellan gum), Locust Bean gum (Locust Bean gum), Tamarind gum (Tamarind gum), gum Arabic (Arabic gum), konjac, pectin, agar, tapioca starch, dextrin, Hydroxypropylmethylcellulose (HPMC), sodium alginate, and modified starch.

In an exemplary embodiment, it may be that the dietary fiber contains one or more selected from the group consisting of citrus dietary fiber and wheat dietary fiber.

In one aspect, the composition according to the present invention has ginseng seed oil as an oil and fat component uniformly mixed with a water-soluble component, so that the ginseng seed oil and the water-soluble component are not separated for a long time, and thus is excellent in stability.

Drawings

Fig. 1 is a graph comparing emulsion stability of example 1 and comparative example 1 according to an aspect of the present disclosure.

Fig. 2 is a graph comparing emulsion stability of example 2 and comparative example 2 according to an aspect of the present disclosure.

Fig. 3A to 3F are graphs illustrating an intra-myocyte energy metabolism promoting effect of ginseng seed oil and ginseng seed extract according to an aspect of the present disclosure.

Fig. 4A and 4B are graphs illustrating a synergistic effect of mitochondrial generation of ginseng seed oil and ginseng seed extract according to an aspect of the present disclosure.

Detailed Description

The present invention is described in detail below.

In one aspect, the present disclosure provides an emulsified composition containing ginseng seed oil and ginseng seed extract. In the embodiments of the present disclosure, the composition contains ginseng seed oil and ginseng seed extract, thereby preventing separation of ginseng seed oil and water-soluble components, which are oil and fat components, and thus can provide a stable emulsified composition.

In one embodiment, the emulsified composition may be a single phase. In the present specification, "single phase" means that an oil phase and an aqueous phase are not separated but remain as one phase in an emulsified composition.

The "oil phase" is a phase that is not easily mixed with an aqueous phase, and may be an oil, an organic solvent, a mixture thereof, or the like as a liquid at room temperature and atmospheric pressure. The "water phase" is not limited as long as it is a phase that is not easily mixed with an oil phase and allows the water-soluble polymer to be easily dissolved. For example, water such as purified water or distilled water, polyhydric alcohol, and the like can be included.

In one embodiment, the ginseng may include one or more selected from the group consisting of Korean ginseng (Panax ginseng), American ginseng (Panax quinfolia), Panax notoginseng (Panax notogeng), Vietnamese ginseng (Panax vietnamensis), Panax japonicus (Panax japonica), Korean ginseng (Panax elegator), Panax quinquefolius (Panax wangianus), Panax notoginseng (Panax bipinnatifidus), and Panax pseudoginseng (Panax pseudo-ginseng), but not limited thereto.

In the present specification, as a method for obtaining the "ginseng seed oil", a squeezing method, a supercritical extraction method, a subcritical extraction method, a microwave extraction method, an ultrasonic extraction method, and the like may be used, but the method is not limited as long as it is obvious to those skilled in the art. The ginseng seeds used in the production of the ginseng seed oil of the present specification include all of the germinated or non-germinated ginseng seeds such as dehisced ginseng seeds, and may be fresh ginseng seeds, or ginseng seeds dried by sunlight drying or hot air drying. For example, in the case of the pressing method, ginseng seed oil can be obtained by placing ginseng seeds or ginseng seed powder in an oil press, which can be used without limitation as long as the oil press is used for food, and pressing the ginseng seeds or ginseng seed powder at normal temperature (about 15 ℃ to 25 ℃) for 3 minutes to 20 minutes. In the supercritical extraction method, ginseng seeds or ginseng seed powder may be placed in a supercritical extraction tank, 50mL to 1L of an extraction solvent is added to 1g of ginseng seed powder, and then a supercritical fluid (e.g., carbon dioxide) is supplied, and then the pressure in the extraction tank is maintained at 300bar to 500bar for extraction for 3 hours to 4 hours.

In one embodiment, the ginseng seed oil may be subjected to a purification process as needed. The purification process is not particularly limited, and any method known in the art may be used. For example, the solvent may be removed by filtration under reduced pressure, ion exchange resin chromatography or silica gel column chromatography, and freeze-drying or concentration.

In the present specification, "ginseng seed" may refer to a fruit obtained during the growth of ginseng. Specifically, it may refer to ginseng that grows from 3 to 5 years, more specifically, fruits that bear fruit for about one week in the middle 7 months among ginseng that grows from 4 years. The method of starting the ginseng used in the present specification is not limited, and the ginseng can be cultivated or purchased in the market. The ginseng seed contains more mineral components including vitamins and ginsenoside than the commonly used ginseng root, and particularly, the ginsenoside contained in the ginseng seed has completely different components from the ginseng root. More specifically, the ginseng seeds contain more PT (Protopanaxatriol) ginsenoside including ginsenoside Re, Rg1, Rg2 and the like than PD (Protopanaxadiol) ginsenoside including ginsenoside Rb1, Rb2, Rc, Rd and the like, and thus can exhibit remarkably superior effects than the ginseng roots.

In the present specification, the "ginseng seed extract" is a broad concept including the following regardless of the extraction method, the extraction solvent, the extracted components, or the form of the extract: the substances obtained by extracting the components of ginseng seeds are all included and include substances obtained by an extraction method including a process of treatment with heat, acid (acid), base (base), enzyme, etc. in the process of extracting the components, and substances obtained by extracting the components of ginseng seeds and then processing or treating them by other methods are all included. Specifically, the processing or treatment may be further fermentation or enzyme treatment of the ginseng seed extract. Therefore, the ginseng seed extract of the present specification may be a fermented product. In the present specification, the ginseng seed extract may be an extract of the following ginseng seeds, specifically, an extract of fresh ginseng seeds or an extract of dried ginseng seeds. In the present specification, fresh ginseng seeds, dried ginseng seeds, or other processed products of ginseng seeds (for example, fermented products, dried pulverized products, etc.) that can be used for the extraction process may be similar or identical in composition to each other. Therefore, fresh ginseng seeds or dried ginseng seeds or other processed products of ginseng seeds having similar or identical components may also exhibit the effects and uses of the ginseng seed extract disclosed in the present specification.

In the present specification, the extraction method for obtaining the "ginseng seed extract" may be heating extraction, cold-soaking extraction, reflux-cooling extraction, ultrasonic extraction, or the like, but is not limited as long as it is an extraction method apparent to those skilled in the art. The ginseng seed used for preparing the ginseng seed extract of the present specification may be a dried ginseng seed, a fresh ginseng seed fruit, or a ginseng seed powder, and any material may be used without limitation as long as it is prepared from a fresh ginseng seed fruit. The ginseng seed extract of the present specification may be an extract of water, an organic solvent or a mixed solvent thereof. The organic solvent may be C₁-C₆Ethanol, acetone, hexane, ethyl acetate, carbon dioxide, or a mixed organic solvent of two or more thereof, but is not limited thereto. The ginseng seed extract of the present specification further contains a substance obtained by concentrating under reduced pressure the extract obtained by the extraction with the extraction solvent. The concentration under reduced pressure may be specifically carried out at 35 ℃ to 50 ℃, but is not limited thereto. When the concentration under reduced pressure is performed at a temperature within the above range, the synergistic effect of the various components in the ginseng seed extract of the present invention is excellent. From the above-mentioned viewpoints, the concentration under reduced pressure may be carried out specifically under conditions of 36 ℃ or higher, 37 ℃ or higher, 38 ℃ or higher, 39 ℃ or higher, 40 ℃ or higher, 41 ℃ or higher, 42 ℃ or higher, and/or 49 ℃ or lower, 48 ℃ or lower, 47 ℃ or lower, 46 ℃ or lower, 45 ℃ or lower, 44 ℃ or lower, and 43 ℃ or lower. The concentration under reduced pressure may be specifically performed under conditions of 600mmHg or more, 620mmHg or more, 640mmHg or more, 650mmHg or more, 660mmHg or more, 680mmHg or more, 700mmHg or more, 720mmHg or more, 740mmHg or more, and/or 760mmHg or less, 740mmHg or less, 720mmHg or less, 700mmHg or less, 680mmHg or less, 660mmHg or less, 650mmHg or less, 640mmHg or less, 620mmHg or less, and 600mmHg or less.

In one embodiment, the composition may contain 0.001 wt% to 70 wt% of the ginseng seed oil, relative to the total weight of the composition. When the ginseng seed oil is contained in an amount exceeding 70% by weight, the stability of the composition may be reduced. Specifically, the composition may contain 0.001% by weight or more, 0.002% by weight or more, 0.003% by weight or more, 0.005% by weight or more, 0.007% by weight or more, 0.01% by weight or more, 0.02% by weight or more, 0.03% by weight or more, 0.05% by weight or more, 0.07% by weight or more, 0.1% by weight or more, 0.2% by weight or more, 0.3% by weight or more, 0.5% by weight or more, 0.7% by weight or more, 1% by weight or more, 1.1% by weight or more, 1.2% by weight or more, 1.3% by weight or more, 1.4% by weight or more, 1.5% by weight or more, 2% by weight or more, 3% by weight or more, 4% by weight or more, 5% by weight or more, 6% by weight or more, 7% by weight or more, 8% by weight or more, 9% by weight or more, 10% by weight or more, 12% by weight or more, 13% by weight or more, or 15% by weight or more, or 13% by weight or more, 70 wt% or less, 69 wt% or less, 68 wt% or less, 67 wt% or less, 66 wt% or less, 65 wt% or less, 64 wt% or less, 63 wt% or less, 62 wt% or less, 61 wt% or less, 60 wt% or less, 59 wt% or less, 58 wt% or less, 57 wt% or less, 56 wt% or less, 55 wt% or less, 54 wt% or less, 53 wt% or less, 52 wt% or less, 51 wt% or less, 50 wt% or less, 49 wt% or less, 48 wt% or less, 47 wt% or less, 46 wt% or less, 45 wt% or less, 44 wt% or less, 43 wt% or less, 42 wt% or less, 41 wt% or less, or 40 wt% or less of the ginseng seed oil.

In one embodiment, the composition may contain 0.01 wt% to 60 wt% of the ginseng seed extract, relative to the total weight of the composition. When the ginseng seed extract is contained in an amount exceeding 60% by weight, the flowability is poor, which may cause inconvenience in the production process. Specifically, the composition may contain 0.01 wt% or more, 0.02 wt% or more, 0.03 wt% or more, 0.05 wt% or more, 0.07 wt% or more, 0.1 wt% or more, 0.2 wt% or more, 0.3 wt% or more, 0.5 wt% or more, 0.7 wt% or more, 1 wt% or more, 2 wt% or more, 3 wt% or more, 5 wt% or more, 7 wt% or more, 9 wt% or more, 10 wt% or more, 11 wt% or more, 12 wt% or more, 13 wt% or more, 14 wt% or more, 15 wt% or more, 16 wt% or more, 17 wt% or more, 18 wt% or more, 19 wt% or more, or 20 wt% or more, and 60 wt% or less, 59 wt% or less, 58 wt% or less, 57 wt% or less, 56 wt% or less, 54 wt% or less, 53 wt% or less, or more, The ginseng seed extract is 52 wt% or less, 51 wt% or less, 50 wt% or less, 48 wt% or less, 46 wt% or less, 44 wt% or less, 42 wt% or less, or 40 wt% or less.

In one embodiment, the weight ratio of the ginseng seed oil and the ginseng seed extract may be 1:2 to 1:100, or 1:2 to 1:50, or 1:2 to 1:15, or 1:7 to 1:13, or 1: 10. When the weight ratio of the ginseng seed oil to the ginseng seed extract exceeds the above range, the oil layer may be separated, thereby reducing the dosage form stability.

In one embodiment, the composition is free of other surfactants. The present disclosure substantially provides an emulsified composition that is stably dispersible without a surfactant. The term "substantially free of surfactant" means that the amount of surfactant in the synthetic ingredients can be minimized or completely free of surfactant, and means that the surfactant is contained in an amount of 1% or less, preferably 0.5% or less, preferably 0.1% or less, and more preferably 0.01% or less, based on the total weight of the composition.

In one embodiment, the composition may further comprise viscose (gum) and dietary fiber.

In one embodiment, the viscose may contain one or more selected from the group consisting of Xanthan gum (Xanthan gum), carrageenan (Carrageenans gum), Guar gum (Guar gum), Gellan gum (Gellan gum), Locust Bean gum (locusts Bean gum), Tamarind gum (Tamarind gum), gum Arabic (arabitic gum), konjac, pectin, agar, tapioca starch, dextrin, Hydroxypropylmethylcellulose (HPMC), sodium alginate, and modified starch.

In one embodiment, the composition may contain from 0.001% to 5% by weight of the viscose, relative to the total weight of the composition. When the viscose is contained in an amount exceeding 5% by weight, a large amount of foam is generated and may cause discomfort in swallowing the composition when it is drunk. Specifically, the composition may contain 0.001 wt% or more, 0.002 wt% or more, 0.003 wt% or more, 0.005 wt% or more, 0.007 wt% or more, 0.01 wt% or more, 0.02 wt% or more, 0.03 wt% or more, 0.04 wt% or more, 0.05 wt% or more, 0.06 wt% or more, 0.07 wt% or more, 0.08 wt% or more, 0.09 wt% or more, or 0.1 wt% or more, and 5 wt% or less, 4.9 wt% or less, 4.8 wt% or less, 4.7 wt% or less, 4.6 wt% or less, 4.5 wt% or less, 4.4 wt% or less, 4.3 wt% or less, 4.2 wt% or less, 4.1 wt% or less, 4 wt% or less, 3.9 wt% or less, 3.8 wt% or less, 3.5 wt% or less, 3.4.4 wt% or less, 3.3.3 wt% or less, 3.1 wt% or less, 3.3 wt% or less, 3.3.3 wt% or less, 3.1 wt% or less, 3.9 wt% or less, 3.3.3.3 wt% or less, 3.1 wt% or less, 3.3.3.1 wt% or less, 3 or less, 3.3.1 wt% or less, 3 or less, 3.3 or less, 3.1 wt% or less, or more, or less, or more, or less, or more, or less, or more, or less, or more, or less, or more, or less, or more, or less, or more, or less, or more, or less, or more, or less, 3% by weight or less, 2.9% by weight or less, 2.8% by weight or less, 2.7% by weight or less, 2.6% by weight or less, 2.5% by weight or less, 2.4% by weight or less, 2.3% by weight or less, 2.2% by weight or less, 2.1% by weight or less, or 2% by weight or less.

In one embodiment, the dietary fiber may contain one or more selected from the group consisting of citrus dietary fiber and wheat dietary fiber.

In one embodiment, the composition may contain 0.001 to 20% by weight of the dietary fiber, relative to the total weight of the composition. When the dietary fiber is contained in an amount exceeding 5% by weight, a large amount of foam is generated and the stability of the dosage form may be lowered. Specifically, the composition may contain 0.001 wt% or more, 0.002 wt% or more, 0.003 wt% or more, 0.005 wt% or more, 0.007 wt% or more, 0.01 wt% or more, 0.02 wt% or more, 0.03 wt% or more, 0.04 wt% or more, 0.05 wt% or more, 0.06 wt% or more, 0.07 wt% or more, 0.08 wt% or more, 0.09 wt% or more, 0.1 wt% or more, 0.2 wt% or more, 0.3 wt% or more, 0.4 wt% or more, or 0.5 wt% or more, and at the same time, 20 wt% or less, 19 wt% or less, 18 wt% or less, 17 wt% or less, 16 wt% or less, 15 wt% or less, 14 wt% or less, 13 wt% or less, 12 wt% or less, 11 wt% or less, 10 wt% or less, 9 wt% or less, 7 wt% or less, 6 wt% or less, 7 wt% or less, or less, 4.8% by weight or less, 4.6% by weight or less, 4.4% by weight or less, 4.2% by weight or less, 4% by weight or less, 3.8% by weight or less, 3.6% by weight or less, 3.4% by weight or less, 3.2% by weight or less, 3% by weight or less, 2.8% by weight or less, 2.6% by weight or less, 2.4% by weight or less, 2.2% by weight or less, or 2% by weight or less.

In one embodiment, the ginseng seed extract may be an extract of water, an organic solvent, or a mixed solvent thereof. The water may include distilled water or purified water, and the organic solvent may be ethanol, acetone, hexane, ethyl acetate, carbon dioxide, or a mixed organic solvent of two or more thereof, but is not limited thereto. The ethanol can be C₁-C₆Low concentration ethanol.

In one embodiment, the composition may be a liquid composition.

In one embodiment, the composition may be a food composition. The formulation of the food composition is not particularly limited, and for example, it may be formulated as tablets, granules, pills, liquid such as drinks, caramel, gel, bars, tea bags, and the like. The food composition of each dosage form can be formulated by appropriately selecting the ingredients generally used in the art, in addition to the effective ingredients, depending on the dosage form or the purpose of use, and can produce a synergistic effect when used together with other raw materials.

The food composition according to one embodiment may contain various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, colorants and accelerators (cheese, chocolate, etc.), pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, ethanol, carbonic acid agents used in carbonated beverages, and the like. In addition, the food composition according to an embodiment may contain natural fruit juice and pulp for preparing fruit juice beverages, vegetable beverages. These components may be used alone or in combination. The ratio of the additives is not critical, but generally ranges from 0 parts by weight to about 50 parts by weight for 100 parts by weight of the composition according to one embodiment.

In one embodiment, the composition may be a muscle energy metabolism-promoting composition.

In one embodiment, the composition may be a fat oxidation promoting composition.

In one embodiment, the composition can be a composition for mitochondrial augmentation and activation.

In one aspect, the present disclosure provides a capsule containing an emulsified composition. The capsule may be a soft or hard capsule.

In one aspect, the present disclosure provides a ginseng seed oil and use of a ginseng seed extract in the preparation of a composition for promoting muscle energy metabolism.

In one aspect, the present disclosure provides a ginseng seed oil and use of a ginseng seed extract in the preparation of a composition for fat oxidation.

In one aspect, the present disclosure provides a ginseng seed oil and use of a ginseng seed extract in the preparation of a composition for mitochondria augmentation and activation.

The present invention will be described in more detail below with reference to examples and the like. It should be clear to a person having ordinary knowledge in the art that the described embodiments are only intended to illustrate the invention and should not be construed as limiting the scope of the invention to the described embodiments.

[ PREPARATION EXAMPLE 1] preparation of ginseng seed oil

Harvesting fresh ginseng (Panax ginseng C.A. Meyer) seeds, separating out the seeds, and harvesting the ginseng seeds. Cleaning the harvested ginseng seeds, removing foreign matters, and screening only the seeds which can be squeezed. Drying, pulverizing, removing impurities, squeezing in oil press (DS-1500, DASAN food industry apparatus, Korea) to obtain Ginseng radix seed oil stock solution. Purifying the extracted ginseng seed oil stock solution, and filtering to obtain the ginseng seed oil.

[ PREPARATION EXAMPLE 2] preparation of Ginseng seed extract

Mixing pulp and fruit juice of fresh radix Ginseng (Panax ginseng C.A. Meyer) seed without peel to obtain radix Ginseng seed material. To 1kg of the ginseng seed material prepared as above, 3L of ethanol was added, and after reflux extraction at 25 ℃ for 4 hours, the ginseng seed extract was obtained by concentration under reduced pressure.

[ PREPARATION EXAMPLE 3] preparation of Red Ginseng extract

1kg of red ginseng material was put into an extractor, and 3L of water and ethanol mixed in a volume ratio of 1:1 were added to the extractor. The extraction was repeated 4 times at 70 ℃ for 10 hours, and the extract was subjected to pressure filtration using a 250 mesh filter. Transferring the liquid into a vacuum concentrator, concentrating at 70 deg.C under 600mmHg, transferring into a sterilizing tank, and steam sterilizing at 83 deg.C for 20 min to obtain Ginseng radix Rubri extract.

[ example 1]

Example 1 was prepared as an emulsified composition of ginseng seed oil and ginseng seed extract according to the components of table 1 below. Specifically, the ginseng seed oil of preparation example 1 was added to the ginseng seed extract of preparation example 2, heated to 70 ℃, and stirred at 200rpm using an Agi mixer. Example 1 was prepared by adding a solution of ginseng seed oil stirred with a ginseng seed extract to purified water and stirring at 200rpm using an Agi mixer (or homogenizer) at 80 ℃.

[ Table 1]

(unit: wt%)	Example 1	Example 2	Comparative example 1	Comparative example 2
					Ginseng seed oil	2.5	2.5	2.5	2.5
Ginseng seed extract	25	25	-	-
					Ginseng radix Rubri extract	-	-	25	25
Gellan gum	-	0.1	-	0.1
					Citrus reticulata dietary fiber	-	0.4	-	0.4
Purified water	To 100	To 100	To 100	To 100
					Total up to	100	100	100	100

[ example 2]

Example 2 was prepared as an emulsified composition of ginseng seed oil and ginseng seed extract containing gellan gum and dietary fiber according to the components of table 1 above. Specifically, the ginseng seed oil of preparation example 1 was added to the ginseng seed extract of preparation example 2, heated to 70 ℃, and stirred at 200rpm using an Agi mixer. Adding gellan gum and citrus dietary fiber into purified water, and heating to 80 deg.C to hydrate gellan gum and citrus dietary fiber. Then, a solution in which ginseng seed oil was stirred to the ginseng seed extract was added to purified water in which gellan gum and dietary fiber were hydrated and reacted, and stirred at 200rpm using an Agi mixer at 80 ℃ to prepare example 2.

Comparative example 1

The composition of comparative example 1 was prepared according to the components of table 1 above. Specifically, the ginseng seed oil of preparation example 1 was added to the red ginseng extract of preparation example 3, and the mixture was heated to 70 ℃ and stirred at 200rpm using an Agi mixer. To the purified water was added a solution of ginseng seed oil stirred with the ginseng seed extract, and stirred at 80 ℃ with an Agi mixer at 200rpm to prepare comparative example 1.

Comparative example 2

The composition of comparative example 2 containing gellan gum and dietary fiber was prepared according to the components of table 1 above. Specifically, the ginseng seed oil of preparation example 1 was added to the red ginseng extract of preparation example 3, and the mixture was heated to 70 ℃ and stirred at 200rpm using an Agi mixer. Adding gellan gum and citrus dietary fiber into purified water, and heating to 80 deg.C to hydrate gellan gum and citrus dietary fiber. Then, a solution in which ginseng seed oil was stirred to the ginseng seed extract was added to purified water in which gellan gum and dietary fiber were hydrated, and stirred at 200rpm using an Agi mixer at 80 ℃ to prepare comparative example 2.

Experimental example 1 confirmation of emulsion stability

The results of mixing immediately after examples 1 and 2 and comparative examples 1 and 2 were confirmed, and after standing at 25 ℃ for 24 hours, the mixture was stirred again for 10 seconds to confirm whether or not the oil phase and the water phase were separated, and the results are shown in table 2, fig. 1, and fig. 2.

[ Table 2]

	Example 1	Example 2	Comparative example 1	Comparative example 2
					Just after preparation	Mixing uniformly	Mixing uniformly	Oil phase separation	Generating a large amount of foam
Standing for 24 hours	Maintaining uniformity	Maintaining uniformity	Oil phase separation	Generating a large amount of foam

As confirmed from the results of table 2, fig. 1 and fig. 2, the examples 1 and 2, which contained ginseng seed oil and ginseng seed extract, were confirmed to have a single phase, in which the oil components were uniformly mixed and maintained stable uniformity even after 24 hours. In contrast, in comparative example 1 and comparative example 2 containing ginseng seed oil and red ginseng extract, the oil component and the water-soluble component were not uniformly mixed, but the oil phase was separated, and in comparative example 2 containing gellan gum, it was confirmed that a large amount of foam was generated instead.

[ Experimental example 2] measurement of the absorption amount of ginsenoside

In order to measure the amount of ginsenoside absorbed, example 1, comparative example 1, the ginseng seed extract (prepared example 2 diluted 4 times with purified water) and the red ginseng extract (prepared example 3 diluted 4 times with purified water) were each passed through a human digestion simulation system, and then the amount of ginsenoside transferred to Caco-2 cells (ATCC) taken from human intestinal cells was measured. Specifically, in the saliva step (salivar phase) of the human digestion simulation system, amylase (2ml) was added to the samples (example 1, comparative example 1, ginseng seed extract, red ginseng extract), followed by stirring at 100rpm for 3 minutes at 37 ℃ and pH6.9, and then in the stomach step (gastrotic phase), pepsin (4ml) was added to the samples passed through the saliva step, followed by stirring at 100rpm for 1 hour at 37 ℃ and pH2(1M HCl), followed by digestion. Then, as a Small intestinal step (Small intestinal phase), lipase, pancreatin, bile acid (bile acid), and amyloglucosidase (amyloglucosidase) were added to the sample digested in the above step, and then the mixture was treated at 37 ℃ and pH5.3(1M NaHCO) to obtain a Small intestinal sample₃) Next, the mixture was stirred at 100rpm for 2 hours. As described above, the sample passed through the human digestion simulation system was centrifuged, the supernatant was diluted 10 times to DMEM, and the processed DMEM was used5% CO at 37 ℃ in a trans well₂Caco-2 cells were cultured for 2 hours under the conditions of (1). After the cultured Caco-2 cells were recovered, the amount of ginsenoside absorbed by Caco-2 cells was measured by High Performance Liquid Chromatography (HPLC), and the results are shown in Table 3 below.

[ Table 3]

As confirmed from the results in table 3, it was found that example 1 containing ginseng seed oil and ginseng seed extract was excellent in the content of ginsenoside absorbed by small intestinal cells and the dosage form stability.

[ Experimental example 3] measurement of expression of genes involved in energy metabolism based on addition of ginseng seed oil

DMEM (Dulbecco' S Modified Essential Medium; Sigma Aldrich) supplemented with 10% fetal bovine serum (total bovine serum; Hyclone), 1% penicillin/streptomycin (penicillin/streptomycin; P/S; Sigma Aldrich) at 5% CO was used₂Myoblasts (myoblasts) obtained from rats purchased from ATCC company were cultured in an incubator (incubator) at 37 ℃. When the number of myoblasts in the Plate (Plate) reached 100%, myotube cells (myotube) were generated by differentiation for one week by a serum starvation (DMEM supplemented with 2% horse serum (Hyclone) and 1% P/S). In myotube cells that completed differentiation, the ginseng seed oil (GS0) of preparation example 1, the ginseng seed extract (GB) of preparation example 2, and the red ginseng extract (RG) of preparation example 3 were treated individually (100ug/ml) or in combination (100. mu.g/ml for each sample and 200. mu.g/ml for the sum of samples) for 16 hours. The positive control group used fenofibrate (100 μ M)) as a PPAR α agonist (agonst) that promotes muscle energy metabolism.

Then, RNA was extracted from myotube cells treated with the above-described sample using a kit (Trizol agent; Invitrogen corporation), and cDNA was synthesized using a RevertAID first strand cDNA Synthesis kit (RevertAID 1st strand cDNA Synthesis kit). Q-PCR (quantitative real-time polymerase chain reaction) was analyzed using CFX96 thermal cycler (thermocycler) of Bio-Rad company, and genes related to energy metabolism (tissue long-chain Acyl-CoA Oxidase (Acyl-CoA Oxidase; ACO), Carnitine-lipid acyltransferase-1 (Carnitine-lipid transferase 1; CPT1), Peroxisome Proliferator-activated Receptor alpha (Peroxisome Proliferator-activated Receptor alpha; PPAR alpha), gamma PPAR co-activated factor 1 alpha (PPAR gamma-coactivator 1 alpha; PGC1 alpha), transcription factor a mitochondria (transcription factor a mitochondria; tfam), uncoupling protein 3 (monoclonal protein 3; P3)) were compared based on the expression level of cyclophilin (cyclophilin) and the relative expression level of RG 3 (RG 3A to RG 3) is shown in the graph, and FIG. 3 shows the relative expression level of RG 3A to RG 3G 3B seed oil, and FIG. 3 shows the relative expression level of RG 3A seed oil, fano stands for fenofibrate).

As shown in fig. 3A to 3F, it was confirmed that the ginseng seed extract has high fat oxidation and expression levels of mitochondrial-related genes relative to the red ginseng extract, and the ginseng seed oil has no particular effect on lipid metabolism in muscle cells. In addition, it was confirmed that the red ginseng extract and the ginseng seed oil showed a synergistic effect, and the gene expression of the red ginseng extract was increased by about 20 to 30% by adding the ginseng seed oil, whereas the ginseng seed extract showed an increasing effect of about 30 to 50% by adding the ginseng seed oil to the ginseng seed extract. Thus, it was confirmed that the ginseng seed oil doubles the energy metabolism promoting effect of the red ginseng extract and the ginseng seed extract, and the synergistic effect is more significant in the ginseng seed extract than in the red ginseng extract. In addition, no increase in energy metabolism based on the treatment of ginseng seed oil was observed in the fenofibrate (fenofibrate) treated group, so that it can be understood that the energy metabolism promoting synergistic effect of ginseng seed oil was specifically shown in red ginseng as well as ginseng seeds.

[ Experimental example 4] based on the increase of ginseng seed oil, increase of mitochondria in myocyte was observed

After treating a sample with the same conditions for myocytes differentiated by the same method as in experimental example 4, the sample was washed 2 times with PBS, and the residual medium was removed. Then, the muscle cells were treated with a mitochondrial Green fluorescent probe (Mitotrack Green FM; Invitrogen corporation) for 30 minutes to stain mitochondria. After 3 additional PBS washing operations, the cells were fixed with formaldehyde (formaldehyde; sigma Aldrich) and observed by a fluorescence microscope (EVOS M5000), and shown in fig. 4A and 4B (in fig. 4A and 4B, GSO represents ginseng seed oil, GB represents ginseng seed extract, RG represents red ginseng extract, and Fano represents fenofibrate).

As shown in fig. 4A and 4B, the phenomenon of increased mitochondria resulting from the treatment of ginseng seed extract was more significant than that of the red ginseng extract treated group, as in the case of increased expression of mitochondrial generation-related genes (PGC1 α, tfam) measured in experimental example 3. In particular, it was observed that in the group in which the ginseng seed extract and the ginseng seed oil were treated together, the mitochondrial density increased to a similar extent to the positive control (fenofibrate) result, and thus it was expected that the ginseng seed oil could actually promote the energy metabolism in the muscle cells.

Formulation example 1 health drink

A healthy beverage was prepared by a general method according to the components described in the following table 4.

[ Table 4]

Formulation of ingredients	Content (wt.)
		Ginseng seed oil	2g
Ginseng seed extract	20g
		Gellan gum	0.1g
Vitamin A acetate	70μg
		Vitamin E	1mg
Vitamin B1	0.13mg
		Vitamin B2	0.15mg
Vitamin B6	0.5mg
		Vitamin B12	0.2μg
Vitamin C	10mg
		Biotin	10μg
Oligosaccharides	2.5g
		Purified water	125ml

The ingredients are mixed according to a method generally used for preparing a health drink, and after stirring at 80 ℃ for about 1 hour, the resulting solution is filtered and sterilized.

[ dosage form example 2] Soft Capsule

Mixing 50mg of ginseng seed oil, 320mg of ginseng seed extract, 60mg of L-carnitine, 2mg of palm oil, 8mg of vegetable hydrogenated oil, 4mg of yellow beeswax and 6mg of soft phospholipid, and filling 1 capsule according to a conventional method to prepare a soft capsule.

Formulation example 3 liquid

A liquid preparation was prepared by mixing 2g of ginseng seed oil, 25g of ginseng seed extract, 3g of isomerized sugar, 1g of mannitol, and 70ml of purified water according to a conventional method for preparing a liquid preparation, filling the mixture in a brown bottle, and sterilizing the bottle.

[ formulation example 4] drink preparation

After 10g of ginseng seed oil, 100g of ginseng seed extract, 10g of glucose, 0.6g of citric acid and 25g of liquid oligosaccharide were mixed, 500ml of purified water was added, and 200ml of each bottle was filled. And (3) after filling the mixture into a bottle, sterilizing the mixture at 130 ℃ for 4-5 seconds, and preparing the beverage.

This application claims priority from korean patent application No. 10-2020-0039315, filed 3/31/2020, which is hereby incorporated by reference in its entirety.

Claims

1. An emulsified composition comprises ginseng seed oil and ginseng seed extract.

2. The emulsified composition according to claim 1,

the ginseng includes one or more selected from the group consisting of Korean ginseng (Panax ginseng), American ginseng (Panax quinquefolia), Panax notoginseng (Panax notogeng), Vietnamese ginseng (Panax vietnamensis), Panax japonicus (Panax japonica), Panax elegans (Panax elegator), Panax quinquefolium (Panax wangianus), Panax notoginseng (Panax bipinnatifidus) and Panax pseudoginseng (Panax pseudoginseng).

3. The emulsified composition according to claim 1,

the Ginseng radix extract is water, C₁-C₆Ethanol or a mixed solvent thereof.

4. The emulsified composition according to claim 1,

the composition comprises 0.001 wt% to 70 wt% of the ginseng seed oil relative to the total weight of the composition.

5. The emulsified composition according to claim 1,

the composition comprises 0.01 wt% to 60 wt% of the ginseng seed extract relative to the total weight of the composition.

6. The emulsified composition according to claim 1,

the weight ratio of the ginseng seed oil to the ginseng seed extract is 1:2 to 1: 100.

7. The emulsified composition according to claim 1,

the composition also contains viscose and dietary fiber.

8. The emulsified composition according to claim 7,

the viscose comprises at least one selected from the group consisting of Xanthan gum (Xanthan gum), carrageenan (Carrageenans gum), Guar gum (Guar gum), Gellan gum (Gellan gum), Locust Bean gum (locusts Bean gum), Tamarind gum (Tamarind gum), Arabic gum (Arabic gum), konjac, pectin, agar, tapioca starch, dextrin, Hydroxypropylmethylcellulose (HPMC), sodium alginate and modified starch.

9. The emulsified composition according to claim 7,

the dietary fiber contains at least one selected from the group consisting of citrus dietary fiber and wheat dietary fiber.

10. The emulsion composition according to any one of claims 1 to 9, wherein,

the composition is a liquid composition.

11. The emulsion composition according to any one of claims 1 to 9, wherein,

the composition is a food composition.