CN110917291A - Composition with function of improving immunity and preparation method and application thereof - Google Patents
Composition with function of improving immunity and preparation method and application thereof Download PDFInfo
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- CN110917291A CN110917291A CN201911275765.5A CN201911275765A CN110917291A CN 110917291 A CN110917291 A CN 110917291A CN 201911275765 A CN201911275765 A CN 201911275765A CN 110917291 A CN110917291 A CN 110917291A
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- lily
- ginseng
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Abstract
The invention provides a composition with an immunity improving function, which is characterized by comprising 5-50 parts by weight of poria cocos; 3-30 parts of lentinus edodes; 2-20 parts of wolfberry fruit; 1.5-20 parts of agaricus blazei; 1.5-20 parts of grifola frondosa; 1.5-20 parts of lily; 1.5-10 parts of ginseng. The composition and the preparation of the invention have the functions of promoting the proliferation of thymus and spleen of mice, improving the capability of phagocytizing inert carbon granules by a reticuloendothelial system of the mice and having obvious in-vivo anti-tumor effect on S180 tumor mice.
Description
Technical Field
The invention relates to the field of medicines, foods or health-care products, in particular to a composition with an immunity improving function and a preparation method and application thereof.
Background
In the current society, with the rapid development of economy, social competition is increasingly intense, the pace of life of people is continuously accelerated, the pressure of work, study and life is continuously increased, a lot of people are busy with remuneration and work all night, the general physical quality is reduced due to overdraft health, overload work, irregular diet time, lack of exercise and the like, the body is in a fatigue state for a long time, and more people are in a sub-health state between health and non-health. The consequence that the organism is continuously in a sub-health state is serious, which causes the reduction of disease resistance and the reduction of immunity, and organic diseases of the body and even tumors are easy to form for a long time, thus seriously threatening the health of people.
Experts at home and abroad point out that the most effective method for eliminating sub-health and preventing diseases is to enhance the immunity of human bodies to diseases, and the key of enhancing the immunity is self-adjustment, diet or Chinese medicine regulation. Modern medical research shows that most edible fungi contain immunoregulation polysaccharide, so that precious mushroom and natural medicinal and edible plants containing the immunoregulation polysaccharide are selected to develop and develop health-care food with the functions of enhancing immunity, resisting tumors and preventing diseases, and intervention is performed when a human body is in a sub-health state with low immunity, so that the immunity of the human body is improved, the probability of occurrence of various diseases is reduced, the life quality of people is ensured, and the physical quality is improved.
Disclosure of Invention
In view of the above, the main object of the present invention is to provide a health food composition with the functions of improving immunity, resisting tumor, removing toxic substance, strengthening body resistance and preventing diseases, and a preparation method thereof.
Specifically, the invention provides a composition with an immunity improving function, which comprises 5-50 parts by weight of poria cocos; 3-30 parts of lentinus edodes; 2-20 parts of wolfberry fruit; 1.5-20 parts of agaricus blazei; 1.5-20 parts of grifola frondosa; 1.5-20 parts of lily; 1.5-10 parts of ginseng. The composition and the raw materials can be directly ground into powder, or can be prepared into extract or other forms by conventional methods.
Further, the composition comprises 15-35 parts of poria cocos; 8-20 parts of shiitake; 5-15 parts of wolfberry fruit; 4-10 parts of agaricus blazei; 4-10 parts of grifola frondosa; 4-10 parts of lily; 2-5 parts of ginseng.
Further, the composition comprises 20-32 parts of poria cocos; 10-16 parts of mushroom; 8-12 parts of wolfberry fruit; 5-8 parts of agaricus blazei; 5-8 parts of grifola frondosa; 5-8 parts of lily; 3-4 parts of ginseng.
Further, the composition comprises 20 parts of tuckahoe; 10 parts of mushroom; 8 parts of wolfberry fruit; 5 parts of agaricus blazei; 5 parts of grifola frondosa; 5 parts of lily; 3 parts of ginseng; or the like, or, alternatively,
32 parts of poria cocos; 16 parts of mushroom; 12 parts of wolfberry fruit; 8 parts of agaricus blazei; 8 parts of grifola frondosa; 8 parts of lily; 4 parts of ginseng; or the like, or, alternatively,
26 parts of poria cocos; 12 parts of mushroom; 10 parts of wolfberry fruit; 6 parts of agaricus blazei; 6 parts of grifola frondosa; 6 parts of lily; 3.5 parts of ginseng.
The invention also provides application of the composition in preparing a medicine, a health-care product or food with the function of improving immunity or resisting tumors.
The invention also provides a medicine, a health-care product or a food containing the composition with the function of improving the immunity.
Specifically, the medicine, health product or food is selected from oral, injection or external preparation. The medicine, food or health care product is prepared from the composition and auxiliary materials or additives acceptable on the medicine, food or health care product.
The adjuvants can be white sugar, lactose, mannitol, sorbitol, erythritol, stevioside, citric acid, malic acid, potassium sorbate, sodium benzoate, sodium carboxymethylcellulose, gelatin, tragacanth, xanthan gum, carrageenan or konjac gum.
Specifically, the medicine, health care product or food comprises but is not limited to oral tablets, buccal tablets, chewable tablets, effervescent tablets, hard capsules, soft capsules, granules, syrup, medicated wine and oral liquid.
Preferably, the oral liquid (which may be in the form of a drink beverage, for example) comprises the composition provided by the present invention, sorbitol and citric acid; the mass percentage concentration of sorbitol in the beverage is 10-20%, and the mass percentage concentration of citric acid is 0.01-0.2%.
The invention also provides a preparation method of the composition, which is characterized by comprising the following steps:
taking poria cocos, shiitake mushroom, wolfberry fruit, agaricus blazei, grifola frondosa, lily and ginseng, removing impurities, cleaning, adding water which is 8-12 times of the total weight of the medicinal materials, boiling and extracting for 2-4 times, wherein the extraction time is 0.5-1.5 h each time, filtering, combining filtrates, concentrating the filtrate at 60-70 ℃ under reduced pressure until the relative density is 1.05-1.10 to obtain a water extraction concentrated solution, refrigerating and centrifuging the concentrated solution.
Further, the preparation of the composition comprises the following steps:
taking poria cocos, shiitake mushroom, wolfberry fruits, agaricus blazei, grifola frondosa, lily and ginseng, removing impurities, cleaning, adding water with the weight being 10 times of the total weight of the raw materials, boiling and extracting for 2 times, wherein the time for boiling and extracting each time is 1 hour, filtering through 120 meshes, combining two filtrates, concentrating the filtrate at 60-70 ℃ under reduced pressure until the relative density is 1.05-1.10 to prepare a water extract concentrated solution, refrigerating the concentrated solution at 0-10 ℃ for 48 hours, and centrifuging at 6000-7000 r/m to obtain the composition.
The invention also provides a preparation method of the medicine, health-care product or food with the function of improving immunity, which is characterized in that when the medicine, health-care product or food is selected from oral preparations, the preparation method comprises the following steps:
taking poria cocos, shiitake mushroom, wolfberry fruit, agaricus blazei, grifola frondosa, lily and ginseng, removing impurities, cleaning, adding water which is 8-12 times of the total weight of the raw materials, boiling and extracting for 2-4 times, wherein the extraction time is 0.5-1.5 h each time, filtering, combining filtrates, concentrating the filtrate at 60-70 ℃ under reduced pressure until the relative density is 1.05-1.10 to obtain a water extraction concentrated solution, refrigerating and centrifuging to obtain the composition; mixing water with the weight 5-10 times that of the composition, the sorbitol and the citric acid with the composition, fully stirring for dissolving, boiling for 30-60 min, cooling, filtering, fixing the volume, refrigerating and centrifuging to obtain a preparation solution; homogenizing the mixed solution, packaging, and sterilizing.
Specifically, the oral preparation comprises the following steps: (1) extraction: respectively taking cleaned poria cocos, shiitake mushroom, wolfberry fruit, agaricus blazei, grifola frondosa, lily and ginseng, adding water which is 8-12 times of the total weight of the medicinal materials, boiling and extracting for 2 times, wherein the extraction time is 1 hour each time, filtering by 120 meshes, and combining the filtrates of the two times; concentrating the filtrate at 65 deg.C under reduced pressure to obtain concentrated solution with relative density of 1.07, refrigerating at 0-10 deg.C for 48 hr, and centrifuging at 7000r/m to obtain composition; (2) blending: mixing water with the weight 5-10 times that of the composition, the sorbitol and the citric acid with the composition, fully stirring and dissolving, boiling for 30-60 min, cooling to room temperature, fine filtering with 200 meshes, fixing the volume, refrigerating the constant volume liquid at 0-10 ℃ for 48h, and centrifuging at 14000r/m to obtain a prepared liquid, wherein the mass percentage concentration of the sorbitol in the prepared liquid is 10-20%, and the mass percentage concentration of the citric acid is 0.01-0.2%; (3) homogenizing: heating the prepared liquid to 60-70 ℃, homogenizing twice in a high-pressure homogenizer at the homogenizing pressure of 30-60 Mpa to obtain a homogeneous liquid; (4) filling: filling and sealing the homogeneous liquid by an automatic filling and sealing machine, and rolling and covering; (5) and (3) sterilization: and (4) filling and sealing the mixture, and then placing the mixture in a sterilizer for sterilization at the temperature of 115-121 ℃ for 20-30 min to obtain the product.
Preferably, when the medicine, health product or food is selected from oral preparation, the preparation method comprises the following steps:
(1) extraction: adding water which is 10 times of the total weight of the medicinal materials into the medicinal materials, boiling and extracting for 2 times, wherein the time for boiling and extracting each time is 1 hour, filtering through a 120-mesh filter, combining filtrates of the two times, concentrating the filtrate at 65 ℃ under reduced pressure until the relative density is 1.07 to prepare a water extraction concentrated solution, refrigerating the concentrated solution at 0-10 ℃ for 48 hours, and centrifuging at 7000r/m to obtain the composition;
(2) blending: mixing 8 times of the weight of the composition of water, sorbitol and citric acid with the composition, stirring for dissolving, boiling for 40min, cooling to room temperature, fine filtering with 200 meshes, fixing the volume, refrigerating the constant volume liquid at 0-10 ℃ for 48h, and centrifuging at 14000r/m to obtain a prepared liquid, wherein the mass percentage concentration of the sorbitol in the prepared liquid is 14%, and the mass percentage concentration of the citric acid is 0.1%;
(3) homogenizing: heating the prepared liquid to 60-70 ℃, homogenizing twice in a high-pressure homogenizer at the homogenizing pressure of 30-60 Mpa to obtain a homogeneous liquid;
(4) filling: filling and sealing the homogeneous liquid by an automatic filling and sealing machine, and rolling and covering;
(5) and (3) sterilization: filling, sterilizing in a sterilizer at 115 deg.C for 30 min.
The composition provided by the invention has the functions of maintaining the balance of functions in vivo, strengthening the body resistance to consolidate the constitution, improving the immunity of the human body, resisting tumors, protecting the health of the human body, preventing invasion of exogenous pathogenic factors and reducing the probability of infection diseases of people, and the composition has reasonable compatibility of the components, can effectively improve the cell repair function of sub-health patients, old people, chronic patients, seriously ill people and the like, promotes the improvement of the immune function of the organism, delays aging and promotes the rapid rehabilitation of the diseases. Wherein the content of the first and second substances,
poria is dry sclerotium of Poria cocos (a fungus in Polyporaceae), and traditional Chinese medicinal materials used as both medicine and food have high compatibility in common clinical formula of traditional Chinese medicine, and the principle is 'ten prescriptions and nine poria cocos'. It has sweet and light taste, mild property, and no damage to vital qi, and has effects of calming heart and tranquilizing mind.
Lentinus edodes is the fruiting body of Lentinus edodes of Pleurotaceae, also known as flower mushroom and Lentinus edodes, and is called "the king of mountain delicacies" and is also a well-known edible and medicinal fungus. It has sweet taste and mild property, and has effects of strengthening body resistance, tonifying deficiency, invigorating spleen, promoting appetite, dispelling pathogenic wind, promoting eruption, eliminating phlegm, regulating qi-flowing, and removing toxic substance.
The medlar is a dry mature fruit of the solanaceae plant medlar, commonly called as the medlar, the red eardrop, the blood medlar and the like, and is a rare plant with homology of medicine and food. The traditional Chinese medicine holds that the wolfberry has mild nature and sweet taste, enters liver and kidney channels, and has the effects of strengthening body resistance, consolidating body resistance, tonifying and nourishing the liver, moistening the lung for improving eyesight, and building up the body.
Agaricus blazei Murill belonging to the genus Agaricus of the order Agaricales of the subphylum Basidiomycotina, also called Agaricus blazei Murill, Pleurotus ostreatus, etc., is a medicinal and edible rare edible fungus, has mild nature and sweet taste, and has effects of invigorating kidney, strengthening essence, strengthening body resistance, consolidating constitution, etc. by entering heart, lung, liver and kidney channels.
Grifola frondosa is fruiting body of Polyporaceae fungus Polyporus Bayes, commonly known as Lyophyllum shiitake, Lyophyllum shiitake and the like, is edible and medicinal mushroom, has mild nature and sweet taste, enters kidney and bladder channels, and has the effects of tonifying deficiency, consolidating constitution, inducing diuresis and relieving swelling.
The lily is dry fleshy scaly leaf of lilium tigrinum, lily or lilium tenuifolia belonging to the liliaceae family, also called lily garlic, is the first product of homology of medicine and food which is passed by the Ministry of health of China, has sweet taste and slightly cold nature, enters heart and lung channels, and has the efficacies of nourishing yin, clearing heat, nourishing essence and blood and the like.
Ginseng is the dry root and rhizome of Panax ginseng of Araliaceae, also known as blood ginseng, Shencao, etc., is a precious and long-standing homologous product of medicine and food, has sweet and slightly bitter taste and mild nature, has the functions of returning heart, lung, spleen and kidney channels, greatly reinforcing primordial qi, recovering pulse, relieving depletion, reinforcing spleen, benefiting lung, promoting the production of body fluid and soothing nerves, and is known as a precious product of the king and the world of various herbs.
Compared with the prior art, the invention has the following beneficial effects:
the composition and the preparation of the invention integrate the mushroom with high nutritive value and immunoregulation polysaccharide and the essence of medicine and food homologous products, and the tuckahoe, the mushroom, the medlar, the agaricus blazei murill, the grifola frondosa, the lily and the ginseng with high safety are scientifically and reasonably matched: wherein Poria is a four-season medicinal preparation with effects of invigorating spleen, eliminating dampness, promoting digestion, and strengthening body constitution; lentinus Edodes and fructus Lycii have effects of strengthening body resistance and tonifying deficiency; agaricus blazei, maitake mushroom, lily and ginseng strengthen body resistance to eliminate pathogenic factors; the raw materials supplement each other, maintain the balance of functions in vivo by regulating the endocrine of the organism, support the healthy energy, combine treatment and nourishment, synergize, improve the immunity of the organism, resist tumors, delay senility, prevent various diseases and regulate the functions of the body.
Detailed Description
The following will clearly and completely describe the technical solutions of the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
A composition for improving immunity and resisting tumor is prepared from the following raw materials in parts by weight: 32 parts of poria cocos; 16 parts of mushroom; 12 parts of wolfberry fruit; 8 parts of agaricus blazei; 8 parts of grifola frondosa; 8 parts of lily; 4 parts of ginseng. The preparation method comprises the following steps: taking the medicinal materials according to the weight parts, removing impurities, cleaning, adding water which is 10 times of the total weight of the medicinal materials, boiling and extracting for 2 times, wherein the time of each boiling and extracting is 1 hour, filtering by 120 meshes, combining filtrates obtained in two times, concentrating the filtrate at 65 ℃ under reduced pressure until the relative density is 1.07 to prepare a water extraction concentrated solution, refrigerating the concentrated solution at 0-10 ℃ for 48 hours, and centrifuging at 7000r/m to obtain the composition (1g is equivalent to 0.61g of crude drug).
Example 2
A composition for improving immunity and resisting tumor is prepared from the following raw materials in parts by weight: 26 parts of poria cocos; 12 parts of mushroom; 10 parts of wolfberry fruit; 6 parts of agaricus blazei; 6 parts of grifola frondosa; 6 parts of lily; 3.5 parts of ginseng. The preparation method comprises the following steps: taking the medicinal materials according to the weight parts, removing impurities, cleaning, adding water which is 10 times of the total weight of the medicinal materials, boiling and extracting for 2 times, wherein the time of each boiling and extracting is 1 hour, filtering through a 120-mesh sieve, combining filtrates obtained in two times, concentrating the filtrate at 65 ℃ under reduced pressure until the relative density is 1.07 to prepare a water extraction concentrated solution, refrigerating the concentrated solution at 0-10 ℃ for 48 hours, and centrifuging at 7000r/m to obtain the composition.
Example 3
An oral preparation for improving immunity and resisting tumor is prepared from the following raw materials in parts by weight: 20 parts of poria cocos; 10 parts of mushroom; 8 parts of wolfberry fruit; 5 parts of agaricus blazei; 5 parts of grifola frondosa; 5 parts of lily; 3 parts of ginseng. The preparation method comprises the following steps: (1) extraction: taking the medicinal materials according to the weight parts, removing impurities, cleaning, adding water which is 10 times of the total weight of the medicinal materials, boiling and extracting for 2 times, wherein the time of each boiling and extracting is 1 hour, filtering through a 120-mesh sieve, combining filtrates obtained in two times, concentrating the filtrate at 65 ℃ under reduced pressure until the relative density is 1.07 to prepare a water extraction concentrated solution, refrigerating the concentrated solution at 0-10 ℃ for 48 hours, and centrifuging at 7000r/m to obtain the composition; (2) blending: mixing 8 times of water, a proper amount of sorbitol and citric acid and the composition, fully stirring and dissolving, boiling for 40min, cooling to room temperature, fine filtering with 200 meshes, fixing the volume, refrigerating the constant volume liquid at 0-10 ℃ for 48h, and centrifuging at 14000r/m to obtain a prepared liquid, wherein the mass percentage concentration of the sorbitol in the prepared liquid is 14%, and the mass percentage concentration of the citric acid is 0.1%; (3) homogenizing: heating the prepared liquid to 60-70 ℃, homogenizing twice in a high-pressure homogenizer at the homogenizing pressure of 30-60 Mpa to obtain a homogeneous liquid; (4) filling: filling and sealing the homogeneous liquid by an automatic filling and sealing machine, and rolling and covering; (5) and (3) sterilization: filling, sterilizing at 115 deg.C for 30min to obtain oral preparation (1ml equivalent to 0.058g crude drug).
Example 4
The efficacy of the experiment for improving the immunity of the organism and resisting the tumor function of the invention is evaluated as follows.
1 materials of the experiment
1.1 Experimental animals and tumor strains
ICR mice, clean grade, 18-22g, male; s180 tumor strain.
1.2 materials and reagents
The composition or oral dosage form prepared in examples 1-3, wherein the concentration of the composition is 0.155 g/mL; 0.9% physiological saline; india ink; 0.1% Na2CO3And (3) solution.
1.3 instruments and devices
Clean bench, electronic analytical balance, type 722 spectrophotometer, sterile surgical instrument, cell counter, cell culture plate, disposable syringe.
2 method of experiment
2.1 Effect of example samples on mouse thymus index and spleen index
36 mice were taken and randomly divided into 3 groups, namely a blank group (normal saline group), an experimental group A (example 1 sample 0.2 ml/d dose group equivalent to 10 times of human recommended dose) and an experimental group B (example 1 sample 0.1 ml/d dose group equivalent to 5 times of human recommended dose), wherein 12 mice in each group were subjected to intragastric gavage once a day by the intragastric gavage method for 12 days continuously, and the mice had free food and water intake. On day 12, the feeding and water supply was stopped, the weight was weighed, the cervical vertebrae were removed, the mice were sacrificed and dissected, the thymus and spleen were removed, surface blood was aspirated, the mass was weighed with an analytical balance, and thymus and spleen indices were calculated.
Thymus (spleen) index (mg/g) ═ thymus (spleen) mass (mg)/mouse body weight (g)
2.2 Effect of the samples of example on the ability of the reticuloendothelial System of mice to phagocytose inert carbon particles in the clear blood stream
36 mice were divided into 3 groups at random, namely a blank group (normal saline group), an experimental group A (example 2 sample 0.2 ml/d dose group) and an experimental group B (example 2 sample 0.1 ml/d dose group), and 12 mice were fed with water and water freely by gavage once a day for 12 days. 24h after the last gastric lavage, injecting Indian ink 10ml/kg diluted 4 times into tail vein of mouse, recording immediately (discarding unsuccessful injection), taking 20 μ l blood from inner canthus vein of mouse 2min and 10min after injecting ink, respectively, and blowing 2ml 0.1% Na2CO3In solution. Killing the mouse by removing cervical vertebra, taking liver, spleen and thymus, weighing the liver, spleen and thymus by using an analytical balance, and adding lyso Na2CO3The solution was shaken up, the absorbance A was measured at a wavelength of 600nm and the phagocytosis index was calculated α.
k＝(lgA1-lgA2)/(T2-T1)
In the formula T1、T2Respectively indicating that ink is injected for 2min and 10 min; a. the1、A2Represents T1、T2The light absorption value at a moment; m represents a bulk mass g; m is1The total mass g of the liver, spleen and thymus is shown.
2.3 in vivo antitumor testing of samples from example
2.3.1 establishment of mouse S180 tumor-bearing model
Aseptically extracting ascites from 7 d-age Holosarcoma S180 transplantation tumor mice, adding physiological saline to adjust tumor cells in cell suspension to 1 × 107And (5) obtaining ascites diluent for later use.
2.3.2 tumor-inhibiting action of the samples of example on S180 tumor mice
36 mice were selected and randomly divided into 3 groups, namely a blank group (normal saline group), an experimental group A (example 3 sample 1.5 ml/d dose group, three times of gavage, 0.5 ml/d dose group each time, 4-6h of gavage each time) and an experimental group B (example 3 sample 0.9 ml/d dose group, three times of gavage, 0.3 ml/d dose group each time, 4-6h of gavage each time), and 12 mice in each group. 0.2ml of ascites diluent is inoculated subcutaneously to the axilla of the right forelimb of each mouse, and after inoculation, the mice are infused with gastric physiological saline or sample liquid within 24 hours for 12 days continuously, and freely eat and drink water. On day 12, the water supply and food supply were stopped, the weight was weighed, the cells were sacrificed by the conventional method, the tumors were taken and weighed, and the tumor inhibition rate was calculated.
3 results of the experiment
3.1 Effect of samples on mouse organ index
See table below.
| Group of | Animals number (n) | Index of thymus | Spleen index |
| Blank group | 12 | 2.25±0.33 | 5.23±0.27 |
| Experiment group A | 12 | 3.12±0.48* | 6.62±0.71* |
| Experiment group B | 12 | 2.91±0.54* | 6.21±0.76* |
Note: p <0.05 compared to saline group.
As can be seen, the thymus index and spleen index of the mice in the experimental group are significantly different (P <0.05) compared with those in the blank group, which indicates that the samples in the examples can obviously increase the thymus and spleen mass of the adult mice.
3.2 Effect of samples on phagocytic index of mouse carbon Clearance
See table below.
Note: p <0.05 compared to saline group.
Therefore, the carbon clearance phagocytosis index of the mice in the experimental group is obviously different from that of the blank group (P <0.05), which indicates that the samples in the examples can improve the phagocytosis index α of the mice and enhance the function of a phagocytosis system.
3.3 Effect of samples on growth of S180 tumor mice
See table below.
Note: p <0.05 compared to saline group.
Therefore, the tumor inhibition rates of the experimental groups are all over 30 percent, and compared with the blank group, the experimental groups have significant difference (P <0.05), which indicates that the samples in the examples have obvious in-vivo anti-tumor effect on S180 tumor mice.
The experimental results show that the composition and the preparation have the effects of promoting the proliferation of thymus and spleen of mice, improving the ability of phagocytizing inert carbon granules by a reticuloendothelial system of the mice and having obvious in-vivo anti-tumor effect on S180 tumor mice, and the composition has the effects of improving immunity and resisting tumor.
The above description of the embodiments is only intended to facilitate the understanding of the method of the invention and its core idea. It should be noted that, for those skilled in the art, it is possible to make various improvements and modifications to the present invention without departing from the principle of the present invention, and those improvements and modifications also fall within the scope of the claims of the present invention.
Claims (10)
1. A composition with the function of improving immunity is characterized by comprising 5-50 parts of tuckahoe by weight; 3-30 parts of lentinus edodes; 2-20 parts of wolfberry fruit; 1.5-20 parts of agaricus blazei; 1.5-20 parts of grifola frondosa; 1.5-20 parts of lily; 1.5-10 parts of ginseng.
2. The composition of claim 1, wherein the composition comprises 15-35 parts of poria cocos wolf; 8-20 parts of shiitake; 5-15 parts of wolfberry fruit; 4-10 parts of agaricus blazei; 4-10 parts of grifola frondosa; 4-10 parts of lily; 2-5 parts of ginseng.
3. The composition of claim 1, wherein the composition comprises 20-32 parts of poria cocos wolf; 10-16 parts of mushroom; 8-12 parts of wolfberry fruit; 5-8 parts of agaricus blazei; 5-8 parts of grifola frondosa; 5-8 parts of lily; 3-4 parts of ginseng.
4. The composition as claimed in claim 1, wherein the composition comprises 20 parts of poria cocos wolf; 10 parts of mushroom; 8 parts of wolfberry fruit; 5 parts of agaricus blazei; 5 parts of grifola frondosa; 5 parts of lily; 3 parts of ginseng.
5. Use of the composition of any one of claims 1 to 4 for the preparation of a medicament, health product or food having an immunostimulating or antitumor effect.
6. A pharmaceutical, health product or food containing the composition having an immunity-enhancing effect according to any one of claims 1 to 4.
7. The medicament, health product or food of claim 6, wherein the medicament, health product or food is selected from oral, injectable or topical dosage forms.
8. The drug, health product or food of claim 7, wherein the drug, health product or food comprises an oral tablet, a buccal tablet, a chewable tablet, an effervescent tablet, a hard capsule, a soft capsule, a granule, a syrup, a medicated wine, an oral liquid.
9. A process for the preparation of a composition according to any one of claims 1 to 4, comprising the steps of:
adding water which is 8-12 times of the total weight of the medicinal materials into poria cocos, shiitake mushrooms, wolfberry fruits, agaricus blazei murill, grifola frondosa, lily and ginseng, boiling and extracting for 2-4 times, wherein the extraction time is 0.5-1.5 h each time, filtering, combining filtrates, concentrating the filtrate at 60-70 ℃ under reduced pressure until the relative density is 1.05-1.10, obtaining a water extraction concentrated solution, refrigerating and centrifuging.
10. The preparation method of the medicine, health-care product or food with the function of improving immunity according to claim 6, wherein when the medicine, health-care product or food is selected from oral dosage forms, the preparation method comprises the following steps:
(1) extraction: adding water which is 10 times of the total weight of the medicinal materials into the medicinal materials, boiling and extracting for 2 times, wherein the time for boiling and extracting each time is 1 hour, filtering through a 120-mesh filter, combining filtrates of the two times, concentrating the filtrate at 65 ℃ under reduced pressure until the relative density is 1.07 to prepare a water extraction concentrated solution, refrigerating the concentrated solution at 0-10 ℃ for 48 hours, and centrifuging at 7000r/m to obtain the composition;
(2) blending: mixing 8 times of the weight of the composition of water, sorbitol and citric acid with the composition, stirring for dissolving, boiling for 40min, cooling to room temperature, fine filtering with 200 meshes, fixing the volume, refrigerating the constant volume liquid at 0-10 ℃ for 48h, and centrifuging at 14000r/m to obtain a prepared liquid, wherein the mass percentage concentration of the sorbitol in the prepared liquid is 14%, and the mass percentage concentration of the citric acid is 0.1%;
(3) homogenizing: heating the prepared liquid to 60-70 ℃, homogenizing twice in a high-pressure homogenizer at the homogenizing pressure of 30-60 Mpa to obtain a homogeneous liquid;
(4) filling: filling and sealing the homogeneous liquid by an automatic filling and sealing machine, and rolling and covering;
(5) and (3) sterilization: filling, sterilizing in a sterilizer at 115 deg.C for 30 min.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911275765.5A CN110917291A (en) | 2019-12-12 | 2019-12-12 | Composition with function of improving immunity and preparation method and application thereof |
| PCT/CN2020/094737 WO2021114586A1 (en) | 2019-12-12 | 2020-06-05 | Immune response-improving composition, preparation method and application thereof |
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Cited By (4)
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| CN112535287A (en) * | 2020-12-07 | 2021-03-23 | 皖西学院 | Compound edible fungus oral liquid and preparation process thereof |
| WO2021114586A1 (en) * | 2019-12-12 | 2021-06-17 | 江苏康仁医药科技开发有限公司 | Immune response-improving composition, preparation method and application thereof |
| CN114848786A (en) * | 2022-04-29 | 2022-08-05 | 江西永昇制药股份有限公司 | Compound Sanshen decoction for resisting cancer and increasing immunity |
| CN115176996A (en) * | 2022-06-22 | 2022-10-14 | 无限极（中国）有限公司 | Composition and powder beneficial to enhancing immunity and application thereof |
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| WO2021114586A1 (en) * | 2019-12-12 | 2021-06-17 | 江苏康仁医药科技开发有限公司 | Immune response-improving composition, preparation method and application thereof |
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| CN114848786A (en) * | 2022-04-29 | 2022-08-05 | 江西永昇制药股份有限公司 | Compound Sanshen decoction for resisting cancer and increasing immunity |
| CN114848786B (en) * | 2022-04-29 | 2024-01-16 | 江西永昇制药股份有限公司 | Anticancer and immunity-increasing compound three-ginseng decoction |
| CN115176996A (en) * | 2022-06-22 | 2022-10-14 | 无限极（中国）有限公司 | Composition and powder beneficial to enhancing immunity and application thereof |
| CN115176996B (en) * | 2022-06-22 | 2024-04-05 | 无限极（中国）有限公司 | Composition and powder for enhancing immunity and application thereof |
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| WO2021114586A1 (en) | 2021-06-17 |
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