CN109275914A - Black fungus polypeptide function edible composition and its preparation method and application - Google Patents

Abstract

The invention discloses a kind of compositions and its preparation method and application for keeping object mixing preparation pair in mind comprising black fungus polypeptide, jujube and ginger.The composition main composition is black fungus micromolecule polypeptide, is easy to absorb, and Efficacy experiments prove that the treatment to a variety of subhealth symptoms has good booster action.The constitutive material of composition is from a wealth of sources, integration of drinking and medicinal herbs, has good market application prospect.

Description

Black fungus polypeptide function edible composition and its preparation method and application

Technical field

The invention belongs to technical field of food science, it is related to the function edible composition that a kind of plant is raw material, more Body is related to a kind of function edible composition and preparation method thereof of extract of complex polypeptide containing black fungus.

Background technique

With world economy high speed development, social rhythm increasingly accelerates, and psychological pressure continues to increase, and environmental pollution damage is tight Weight, causes majority to be in sub-health state, in terms of there is psychoneural aspect, treating cardiac and cerebral vascular diseases, digestive system and moves The activity that system aspects are related to multiple systems deviates normal condition.When human body is in sub-health state, on cellular level, show as Na+, K+-ATP enzymatic activity are suppressed on cell membrane or inactivate, and are not able to satisfy the exchange of intraor extracellular potassium, sodium ion, influence film electricity Position and cause contractile function to reduce；The molecules transmembrane transports such as glucose, fatty acid and lactic acid are reduced, endocellular metabolism function is influenced Can, hinder on film ion exchange, reduction Na+, Ca2+-ATP inside and outside H+ and the films such as lactate transhipment, Cl-/HCO3-, Na+/H+ Enzymatic activity makes Ca2+ conveying be obstructed, and superoxide radical increases, and changes polypeptide, configuration of catecholamine hormone receptor etc. on film Deng eventually leading to the generation and development of various diseases so as to cause the sub-health state of human body.Sub-health state seriously affects People's lives quality affects our work and cause.Improve inferior health constitution and reverts to health status as the whole world And the important health care demand in China.

Although there is the product of the improvement inferior health constitution of a multitude of names on the market at present, most expensive starting materials have Preparation process it is complicated, some tastes are bad, and the design feature and product component system of some product components cause the product to inhale The symptom index that rate of producing effects is undesirable, conditioning is improved is few.In short, in the function edible composition technical field of Subhealth treating, , there is still a need for continually developing raw material to be easy to get, simple process is in good taste for we, and absorption efficiency is high, improves more than symptom type Healthcare function product.

Black fungus is China's Subhealth treating food materials important since ancient times.Containing colloid protein and water-soluble fibre, black wood Ear colloid albumen and water-soluble fibre can clear up enteron aisle rubbish, increase intestines peristalsis, treat constipation, for cleaning blood vessel and clearly Except lung's rubbish has remarkable result.It is the health care product of the improvement inferior health of the dust pollutions industries such as haircut production, textile worker.But The black fungus of edible routine form is unfavorable for intestinal absorption, and eating human absorptivity as vegetable is only 5-8%.

Modern nutriology has many physiology function the study found that plant polypeptide is lower-molecular substance important in organism Can, in addition to the precursor as RNA, DNA, also as Biochemical processes Auto-regulator participate in vivo be metabolized, eucaryotic cell structure, It plays an important role in terms of metabolism and regulatory function.Therefore black fungus polypeptide is then a kind of important Subhealth treating polypeptide.

There is jujube QI invigorating to support kidney, blood-enriching face-nourishing, tonifying liver decompression, soothing nerves, tonifying yang, control the effect of consumptive disease damage." therefore jujube is pole Good tonic food.Li Shizhen (1518-1593 A.D.) is said in Compendium of Material Medica [1]: jujube is sweet in flavor, warm-natured, and energy tonifying middle-Jiao and Qi, nourishes blood and promotes the production of body fluid are used for The diseases such as treatment " spleen deficiency weak, anorexia and loose stool, deficiency of qi and blood ".It is recorded in the herbal medicine books " this warp " of China, jujube property sweet in flavor Temperature, returns spleen stomach meridian have tonifying middle-Jiao and Qi, nourishing blood and tranquilization, the function of mitigating pharmacological property；And modern pharmacology then finds that jujube contains The nutritions abundant such as protein, carbohydrate, organic acid, vitamin A, vitamin C, lot of trace calcium and amino acid, jujube energy It improves the immunity of the human body, and can inhibit cancer cell.Pharmacological research discovery, jujube can promote the generation of leucocyte, reduce serum gallbladder Sterol improves seralbumin, protects liver, also contains in jujube and inhibit cancer cell, or even cancer cell can be made to normal cell The substance of conversion.Rutin contained by jujube can make angiomalasia, to reduce blood pressure, have prophylactic-therapeutic effect to high blood pressure.Jujube is rich Containing the ingredients such as protein, fat, carbohydrate, carrotene, B family vitamin, vitamin C, citrin and phosphorus, calcium, iron, wherein tieing up The content of raw element C comes out at the top in fruit, there is the laudatory title of " natural vitamin ball ".With stronger tonic effect, can improve Immune function of human body enhances disease resistance；Containing flavones -- double-glycoside A has calm, hypnosis and antihypertensive effect, wherein shaddock Aglycon C glucoside has central inhibitory action, i.e. reduction autogenic movement and stimulation reflex, tetanic numb effect, therefore jujube has There is the function of tranquilizing the mind, calmness；The cyclic adenosine monophosphate being rich in is the necessary material of human energy metabolism, can enhance muscular strength, eliminate it is tired Labor, increases myocardial contractive power, improves myocardial nutrition expansion blood vessel, has good effect to prevention and cure of cardiovascular disease.Jujube has Tonic and nourishing, nourishing blood and tranquilization, strengthening the spleen and stomach and other effects.Jujube is to diseases such as acute, chronic hepatitis, cirrhosis, anaemia, anaphylactoid purpuras There is good therapeutic effect.Jujube contains triterpene compound and cyclic adenosine monophosphate, there is stronger suppression cancer, anti-allergic effects, to prevention and treatment bone Matter is loose, postpartum anemia plays an important role.

(3) the effect of ginger: cardiac stimulus and blood vessel

Rich in gingerol, there is certain stimulation to heart and blood vessel, palpitating speed, blood vessel dilatation can be made, to make Channels is unobstructed, and supply is normal.

In Subhealth treating function edible composition field, not yet occur commercialized including black fungus polypeptide, and compound The complex function edible composition composition of jujube and ginger effective active composition.

Summary of the invention

Deficiency that is existing and seeming is made up the object of the present invention is to provide a kind of, a kind of raw material is easy to get, simple process, In good taste, absorption efficiency is high, and the function comprising black fungus complex polypeptide of improving the improvement inferior health more than symptom type eats group Close object and preparation method thereof and medical usage.

For achieving the above object, a technical solution of the invention is a kind of function edible composition, and it includes black Agaric polypeptide extract, Fructus Jujubae extract and Ginger P.E.Wherein, the black fungus polypeptide, Ginger P.E and jujube mention The weight ratio for taking object is 10~20 parts: 30~60 parts: 5~10 parts.

Wherein, the weight ratio of the black fungus polypeptide, Fructus Jujubae extract and Ginger P.E is 10~20 parts: 30 ~60 parts: 5~10 parts.

Second scheme of the invention is kind of the function comprising black fungus polypeptide extract, Fructus Jujubae extract and Ginger P.E The preparation method of energy edible composition, compound protease decompose black fungus raw material and obtain black fungus complex polypeptide enriched product, match With jujube and Ginger P.E, the function and service object for improving human-body sub-health is made.Preparation method includes: processing homogenate, carefully Born of the same parents are broken, digest, and extract filtering, add jujube, ginger extract in proportion, dry after mixing, must can be used for Subhealth treating Function composition dry powder formulations.Including step

(1) black fungus small-molecular peptides solution is prepared；

(2) jujube and ginger mixed extract are prepared respectively；

(3) by black fungus small-molecular peptides solution, jujube extracting solution and ginger extract according to 10~20 parts::

30~60 parts: 5~10 of weight ratio mixing preparation；

(4) mixture drying is formed into powder mixture.

(5) using step (4) resulting powder mixture as additive, function edible composition is prepared.

In one embodiment, the function edible composition is oral solution.

In one embodiment, the step (5) includes such as colored, filling, pasteurization, labeling.

In some embodiments, in the step (2) jujube extracting solution extracting method comprising steps of cleaning, cleaning, Thermophilic digestion, mashing, filtering, colloid mill, homogenate.

In some embodiments, in the step (2) ginger extract extracting method comprising steps of cleaning, juicing, Filtering.

In some embodiments, the compound small-molecular peptides of the black fungus preparation method comprising steps of

Pre-treatment

(1) physics is broken；

(2) pyrohydrolysis；

(3) it is homogenized；

(4) it sterilizes；

Digest black fungus polypeptide

(4) compound protease, heat preservation hydrolysis are added；

(5) it is centrifuged, separation and fermentation liquid；

Drying and moulding and packing

(6) it is spray-dried；

(7) it pelletizes, packing.

In some embodiments, the sieving is that the black fungus after Mechanical Crushing is crossed 20~60 meshes.

In a specific embodiment, the sieving is that the black fungus after Mechanical Crushing is crossed 35 meshes.

In some embodiments, the temperature of the pyrohydrolysis is 80 to 100.

In one embodiment, the temperature of the pyrohydrolysis is 100.

In some embodiments, the temperature of the pyrohydrolysis is 80 to 100

In some embodiments, the time of the pyrohydrolysis is 1 to 6 hours.

In a specific embodiment, the time of the pyrohydrolysis is 3 hours.

In some embodiments, the homogenate is that the black fungus after pyrohydrolysis acts on 0.5 in grinding homogenate equipment ~2 hours.

In a specific embodiment, the homogenate is that the black fungus after pyrohydrolysis acts on 1 in grinding homogenate equipment Hour.

In a specific embodiment, the compound small-molecular peptides of the black fungus be decomposed by compound protease it is pretreated Broken agaric obtain.

Efficient proteolytic enzyme should have other than answering percent hydrolysis with higher to substrate protein white matter using peace Entirely, cheap, the features such as working condition is of less demanding.Enzyme is as a kind of with physiological activity

Specific proteins, active centre is different, and the corresponding specificity that hydrolyzes is also different, selects not to same protein Same protease is hydrolyzed, and hydrolysis effect has very big difference.Different according to the pH scope of application, test uses neutral protein Two kinds of enzyme united hydrolysis of enzyme and acid protease, to obtain high percent hydrolysis.

In some embodiments, the proteinase complex includes albumen excision enzyme, protein incision enzyme and food flavor enzyme.

In some embodiments, the protease includes animal origin, plant origin and microbe-derived.

The protease of the animal origin includes trypsase, pepsin and chymotrypsin；The plant origin Protease include papain, bromelain and ficin；The microbe-derived protease includes type Bacillus 2709, actinomyces 166, rare earth aspergillus 3.942, As1.389 and As3.350.

In some embodiments, the protein incision enzyme is in comprising papain, bromelain, 1.39 The group of property protease, 2709 alkali proteases and acid protease.

In some embodiments, the albumen excision enzyme, which is selected from, includes aspergillus oryzae excision enzyme, aspergillus niger excision enzyme and rice The group of bent black bent excision enzyme.

In some embodiments, the food flavor enzyme includes yeast flavor enzyme, lactic acid bacteria food flavor enzyme and hay bacillus flavor Enzyme.

In some embodiments, the proteinase complex is selected from pawpaw restriction endonuclease, bromelain, 1.398 neutrality It is two or more in protease, 2709 alkali proteases, acid protease, aspergillus oryzae excision enzyme and yeast flavor enzyme.

In some embodiments, the proteinase complex is by aspergillus oryzae excision enzyme, pawpaw restriction endonuclease and yeast flavor Enzyme composition.

Concentration of substrate, enzyme concentration, hydrolysis temperature, pH value, the condition appropriate such as time be influence hydrolysis result it is main because Element.

In some embodiments, the Mole percent composition ratio of the pawpaw restriction endonuclease and yeast flavor enzyme is 1:10 ~10:1.

In some embodiment party examination, the dosage of the aspergillus oryzae excision enzyme is 1000U/g, the dosage of the pawpaw restriction endonuclease For 1000U/g, the dosage of the food flavor enzyme is 100U/g.

In a specific embodiment, the Mole percent composition ratio of the pawpaw restriction endonuclease and yeast flavor enzyme is 4: 4:2。

In some embodiments, it includes circumscribed enzyme effect that the proteolytic enzyme complex hydrolyses black fungus, which is homogenized, interior Enzyme cutting effect.

In some embodiments, the proteolytic enzyme complex hydrolyses black fungus homogenate further includes food flavor enzyme three steps Suddenly.

In some embodiments, the circumscribed enzyme action time is 6~20 hours, and the inscribe enzyme action time is 20 ~100 hours, the flavor enzyme action time was 10~20 hours.

In some embodiments, the circumscribed enzyme action time is 12 hours, and the inscribe enzyme action time is 60 small When, the flavor enzyme action time is 15 hours.

In some embodiments, the effect pH value of the protein incision enzyme is 6.0~7.0.

In one embodiment, the effect pH value of the protein incision enzyme is 6.5.

In some embodiments, the operative temperature of the albumen excision enzyme is 50 degree to 60 degree.

In some embodiments, the operative temperature of the protein incision enzyme is 50 degree to 60 degree.

In some embodiments, the operative temperature of the albumen food flavor enzyme is 50 degree to 60 degree.

In some embodiments, the heating method is steam heating.

In some embodiments, the heating method is microwave heating.

In some embodiments, the additional amount of the albumen excision enzyme is the every 200g black fungus of 30`80g protease.

In some embodiments, the compound small-molecular peptides of the black fungus are to ferment to obtain by composite bacillus.? In one embodiment, the bacillus compound bacteria is made of bacillus subtilis and bacillus licheniformis, and the spore bacillus is multiple The thallus number ratio of careless bacillus and bacillus licheniformis in combined bacteria is 1::10~10:1.

Bacillus subtilis is the widely applied a kind of bacteriums in fields such as industry, agricultural and medical industry, neutral proteinase A kind of important products raw as producing bacillus subtilis, the application in food-processing industry increasingly causes people in recent years Pay attention to.Bacillus cereus is a kind of critical strain for generating alkali protease.

Black fungus homogenate obtains polypeptide product after enzymatic hydrolysis to be had since the physicochemical property of black fungus includes

1) low viscosity under good dissolubility and high concentration；

2) good and acidproof and heat-resistant stability；

3) black fungus polypeptide Instant is good, and NS1 value is up to 85%.；

4) black fungus polypeptide has good hygroscopicity, moisture retention and gel softening function.These good reasons

Changing characteristic is that black fungus polypeptide has the high property digested and assimilated, high antioxidant, low antigenicity, has protection and improves The multiple functions such as function of human body, skin care.Third technical solution of the invention is that polypeptide extract containing black fungus, jujube mention Take application of the function and service object of object and Ginger P.E in conditioning subhealthy state symptom.

In some embodiments, the effect of the conditioning subhealthy state includes

1, active absorption restores rapidly physical efficiency

Human body just needs to consume the amino acid in muscle when carrying out vigorous exercise in order to feed energy.That is body It needs to obtain from muscle when energy.After amino acid in muscle is consumed, musculature will be damaged, Muscle generates feeling of fatigue, is difficult to play original strength.Active peptide can more quickly be absorbed by the body than amino acid.Restoring body In terms of power, active peptide also plays excellent effect.

In some embodiments, the effect of the conditioning subhealthy state includes

2, brain fag is eliminated

Caused by brain fag is the rising as oxygenated haemoglobin concentration, taking agaric active peptide can inhibit to aoxidize blood The rising of hemoglobin concentration, it is thus possible to mitigate the stress of brain.

In some embodiments, the effect of the conditioning subhealthy state includes accelerating lipolysis:

Peptide has the function of hindering fat absorption, accelerates lipolysis, promotes basic metabolism, can increase energy after diet Consumption, promote body fat burning.After the intake of black fungus oligopeptide, blood is rapidly entered, sporter can be met to albumen The metabolic demand of matter prevents muscle, skin, blood etc. because of the generation of movement consumption albumen, muscle damage phenomenon.Active peptide can be with It solves the problems, such as " supplementing protein within the growth hormone secretion vigorous period ", there is quick, entirety to be absorbed function Feature

In some embodiments, the effect of the conditioning subhealthy state includes mitigating to move caused muscle damage

Active peptide is compared with amino acid, and faster by the speed of intestinal absorption, edible jelly fungi peptide can be rapid after movement for active peptide Ground supplement protein, the muscle damage as caused by SRT load and resulting myalgia, swelling are all substantially reduced.Pass through The continuous repetition of circulation, muscle will become more and more strongr.

In some embodiments, the effect of the conditioning subhealthy state includes adjusting blood lipid, norcholesterol

After taking in active peptide, the intracorporal thyroid body of people can be stimulated, increases thyroid hormone secretion amount.Thyroid hormone It is external to have the function of that the acidification of promotion cholesterol, obstruction intestinal wall accelerate to exclude to cholesterol absorption.Active peptide is for reducing people Body cholesterol has selection index system characteristic, and when body's cholesterol is under normal state of value, intake active peptide is to cholesterol without drop Inefficient to answer, after high cholesterol patient takes in small-molecular peptides, then generating cholesterol reduces effect, and reduced Lipoprotein classes category It is beneficial to human body, blood lipid molecule can be carried decomposed to liver, exclude body in the low-density lipoprotein being harmful to the human body Outer high-density lipoprotein effect.Active peptide can increase the activity of mitochondria inside brown fat, promote fat metabolism.It can also increase Add the conversion ratio of norepinephrine, the inhibition to lipase is reduced, to promote fat metabolism.Take in black fungus small molecule Peptide can also play the role of preventing serum cholesterol and blood lipid raised.Show in the culture experiment made of enterocyte: active peptide Pass through the DNA structure for invading cholesterol cell, destroying cholesterol, it is suppressed that the proliferation of cholesterol.

In some embodiments, the effect of the conditioning subhealthy state includes improving the effect of immunity

Contain the amino acid that immunity can be improved in active peptide.It is wherein representative to be exactly: arginine and paddy ammonia Acid.The immune function of the macrophage cell in immunocyte can be enhanced in arginine, while attacking invading virus.In addition, When a large amount of Virus entry human bodies, glutamic acid can produce immunocyte, beat back virus.So active peptide can improve machine The cellular immune function of body, it can promote T lymphocyte to be proliferated, and enhance the function of macrophage, enhance the activity of NK cell. Research report, active peptide can also promote the generation of tumor necrosis factor.

In some embodiments, the effect of the conditioning subhealthy state include be particularly suitable for small intestine occur functional disorder and Postoperative patient is edible.

Black fungus active peptide can increase mucous membrane of small intestine height of naps, deepen Crypt depth, increase the absorbing surface of intestinal mucosa Product promotes small intestine body of gland flourishing, increases the activity of amino acid peptase, therefore can promote the improvement of Small Intestinal.Small When dysfunction occurs for intestines, the absorption function of active peptide is barely affected, and has high healthy nutritive value.In addition, art The enteron aisle of patient is easy to be exposed under the infringement of pathogen afterwards, and active peptide can prevent pathogen from invading intestinal mucosa.

In some embodiments, the effect of the conditioning subhealthy state includes blood pressure lowering

Active peptide antihypertensive effect is steady, is not in the fluctuation in drug pressure reduction, is also depressured and endangers without drug The side effect of other organs such as evil liver, kidney, has significantly more effect particularly with primary hypertension patient.

In some embodiments, the effect of the conditioning subhealthy state includes that buffering blood glucose rises

Active peptide is the effective component for constituting alpha-glucosidase inhibin.Contain a kind of alpha-glucosaccharase in human small intestine Enzyme, major function are the various sugar that fast decoupled enters small intestine, supply demand of the human body to glucose.α-Portugal that active peptide is constituted Polyglycoside enzyme inhibin, which has, to be prevented, alpha-glucosidase is delayed to make the degradation of the carbohydrate such as starch, sucrose, oligosaccharide With, alleviate carbohydrate to glucose conversion, into blood, lead to blood glucose rise phenomenon, to reach reduction blood glucose target Purpose, prevent Blood Glucose rise caused by complication.

In some embodiments, the effect of the conditioning subhealthy state includes anti-oxidant, anti-aging

Active peptide can be improved the activity of superoxide dismutase, glutathione peroxidase, anti-lipid peroxidation, Scavenging hydroxyl advantageously reduces tissue oxidizing, protects body.Safeguard that cell eubolism, delaying cell aging make one to prolong Year lengthens one's life.Accelerate damaged cell reparation simultaneously, keep human body vigor, reduce pigmented generation, to prevent and postpone the " longevity Spot " appearance.

The another technical solution that the present invention takes is: a kind of medical usage of compound plant polypeptide, based on black fungus Raw material is wanted, low temperature extracts complex polypeptide, is equipped with jujube and closes pharmaceutical preparation made from ginger extract, for adjusting sub-health population Symptom, effectively improve sub-health status, enhance reserve, the resistance, restoring force of body, promote sub-health population to strong Health conversion, prevents sub-health population from developing to disease direction.

In some embodiments, the effect of the conditioning subhealthy state includes antiallergy

Active peptide is the elderly that convalescent, digestive function decline, especially to the infant of cow milk protein allergy, And easily cause food hypersenstivity people provide safety, without anaphylactoid nutrient protein source.

In some embodiments, the effect of the conditioning subhealthy state includes that accelerating alcohol decomposes, and reduces the murder by poisoning of alcohol Effect

In some embodiments, the effect of the conditioning subhealthy state includes the alanine and leucine improved in blood Content can effectively reduce the concentration of alcohol in blood under the collective effect of alanine and leucine, and accelerating alcohol decomposes, Reduce the toxic action of alcohol.Leucine can make the alcohol for gathering brain tissue, reduce to the paralysis of bone and the work of abnormal contraction With reducing the generation of the alcoholism phenomenon such as behavioral disorder, aphasia of alcohol user.

In some embodiments, the effect of the conditioning subhealthy state includes improving attention

The present invention uses biotechnology, and a kind of micromolecule polypeptide is extracted from black fungus cell, can increase cell membrane Na+, K +-atpase activity is conducive to body synthesis ATP to cell energy and power is provided and promotes intraor extracellular mass exchange ability, Accelerate metabolism, the acidic materials and superoxide radical accumulated in vivo are discharged, transfer cell viability, improves cellular sodium-potassium ATP The activity of enzyme, it is anti-oxidant, comprehensive adjustment sub-health population psychosoma lack of proper care the stage fatigue, insomnia, etc. symptoms, promote inferior health to Health conversion improves people's health level and quality of life.And raw material sources are extensive, integration of drinking and medicinal herbs, main composition Using the black fungus complex polypeptide for the high-purity that black fungus is prepared by extraction, cooperate the concentrated extract of jujube and ginger The resulting composition of mixing preparation has efficient booster action to the treatment of a variety of inferior health diseases, therefore has extremely good Commercial promise.

Detailed description of the invention,

The Capillary Electrophoresis result for the compound protein enzymatic hydrolysis that the broken homogenate of Fig. 1 black fungus is generated by ferment

The molecular weight standard of Fig. 2 polypeptide

Fig. 3 standard molecular weight working curve

Fig. 4 albumen microwave acidolysis peptide-based gel permeation chromatography

Specific embodiment

The following examples are used to further illustrate the present invention, without that should limit the scope of the present invention:

Embodiment one: the preparation of black fungus polypeptide dry powder

1. taking 100 kilograms of black fungus, select, clean, is crushed in mechanical roller.It is added 5 times 55 DEG C of black fungus weight Distilled water is crushed black fungus historrhexis and is homogenized 2 hours in high pressure homogenizer.

2. using 6.0mol/L HCl solution, pH value 5.5 is controlled.It is 5% according to substrate mass concentration, by aspergillus oryzae excision enzyme Dosage 8%, Papain enzyme solution dosage 15%, under 55 DEG C of constant temperatures, aspergillus oryzae flavor enzyme dosage 5%, successively with being outer Enzyme cutting effect 20 hours, Papain enzyme effect 60 hours, aspergillus oryzae flavor enzyme effect 10 hours.

3. being cooled to room temperature, 3000r/min is centrifuged 5 minutes, the enzyme deactivation 50 minutes of 70 DEG C of supernatant, is concentrated under reduced pressure, is obtained more Peptide concentrate；55 DEG C of distilled water of 3-5 times of residual residue addition will be centrifuged, carry out 2 enzymic digestions, method and 2 phase of above step Together.

(6) isolate and purify, with specific process, by small-molecular peptides liquid residual enzyme and other materials separate, small point Sub- peptide liquid, which extracts, to be completed, and molecular weight is in 180 to 480 dalton.

(7) it is arranged with reverse osmosis concentration and removes moisture, become wet-milling, with the drying of microwave vacuum machine or is spray dried to do Powder.

(8) particle of certain specification is made of granulator black fungus small molecule active peptide particles, is packed with vacuum packing machine At required various quantitative packages.

4. step 3 gained concentrate is mixed well, it is spray-dried, granule or tablet, capsule is made；

5. the packaging of granule: 5g/ bags, 20 bags/box.

Usage and dosage: once in the morning and once at night, 1 bag every time, warm boiled water.

Two, of embodiment

Embodiment one: the preparation of black fungus polypeptide dry powder

1. taking 100 kilograms of black fungus, select, clean, is crushed in mechanical roller.It is added 5 times 55 DEG C of black fungus weight Distilled water is crushed black fungus historrhexis and is homogenized 2 hours in high pressure homogenizer.

2. using 6.0mol/L HCl solution, pH value 5.5 is controlled.It is 5% according to substrate mass concentration, by aspergillus oryzae excision enzyme Dosage 8%, Papain enzyme solution dosage 15%, under 55 DEG C of constant temperatures, aspergillus oryzae flavor enzyme dosage 5%, successively with being outer Enzyme cutting effect 20 hours, Papain enzyme effect 60 hours, aspergillus oryzae flavor enzyme effect 10 hours.

3. being cooled to room temperature, 3000r/min is centrifuged 5 minutes, the enzyme deactivation 50 minutes of 70 DEG C of supernatant, is concentrated under reduced pressure, is obtained more Peptide concentrate；55 DEG C of distilled water of 3-5 times of residual residue addition will be centrifuged, carry out 2 enzymic digestions, method and 2 phase of above step Together.

(6) isolate and purify, with specific process, by small-molecular peptides liquid residual enzyme and other materials separate, small point Sub- peptide liquid, which extracts, to be completed, and molecular weight is in 180 to 480 dalton.

(7) it is arranged with reverse osmosis concentration and removes moisture, become wet-milling, with the drying of microwave vacuum machine or is spray dried to do Powder.

The detection of two, black fungus polypeptide of embodiment

1 materials and methods

The black fungus protein isolate homogenate of 1.1 raw materials and instrument embodiment one, protein content >=89%；Neutral protein Enzyme, Wuxi bioengineering Co., Ltd of Jie Neng section avenge plum board (food-grade)；Alkali protease, Wuxi Jie Neng section bioengineering have Limit company avenges plum board (food-grade)；Polypeptide molecular weight standard, Amersham Bio2sciences company, Capillary Electrophoresis are special With；Micro-wave oven, lg electronics Electrical Appliances Co., Ltd, MS22089T；Formaldehyde, Xi'an chemical reagent factory are analyzed pure；Efficient capillary electricity Swimming instrument, CE2212, Peking University's manufacture；Gel permeation chromatograph, 24142515, U.S. Waters.

1.2 test methods take 8g black fungus to separate homogenate, and 90g water is added, mixes well, and adjust solution ph to 8.0, are added 0.35g neutral proteinase, microwave 150W heat 1min, take out and cool down and supply moisture, place into microwave stove heating 1min, repeatedly For several times, 40~45 DEG C of such temperature control, is heated repeatedly, and until pH value is constant, solution ph is 7.0 at this time；Adjust solution ph To 3.5,0.3 g acid protease is added, microwave 150W heating, is heated repeatedly by 45~50 DEG C of temperature control, until pH value is constant, PH value is 2~3 at this time；It takes out spare for detection.

Detection method

1. the detection of degree of hydrolysis and ammonia nitrogen is with formol titration (GB/T 14771-1993).The definition of degree of hydrolysis is original The percentage that peptide bond is cleaved in material protein, formula are as follows: DH=h/htot × 100%=(CNH2/CSPIW)-C0NHI/ Htot × 100%；It is the peptide bond number of material protein that h, which is albumen is cleaved after hydrolyzing peptide bond number mmol/g, htot, in formula mmol/g；It is a constant, black fungus albumen htot=8.8mmol/g for a certain specific protein htot；CNH2 is water The content mmol/L, CSPIW for solving amino-acid nitrogen in protein liquid are the content mg/ml, C0NH2 of protein in hydrolyzed protein liquid The content mmol/g of free amino nitrogen in material protein；To black fungus albumen, value is 0. 43mmol/g.

2. the detection of polypeptide surveys its molecular weight using Capillary Electrophoresis and gel permeation chromatography

The detection of 2 high performance capillary electrophoresis

1) detection of polypeptide.Protein breakdown object is dissolved in (5%22 sulfydryl second of 60mmol/L Tris/HCl+1%SDS+ Alcohol, pH value=6.0) mixed liquor in, set 5min in boiling water, be centrifugated 10 min (4 000r/min), supernatant is taken to pass through 0.45 μm of membrane filtration, sample introduction is analyzed after ultrasonic degassing.Quartz capillary respectively with distilled water, 0.1mol/L NaOH, 0.1mol/L HCl rinse 3min after, with buffer (100mmol/L Tris/HCl+0.1%SDS+0.1%PEO, pH value= 8.5) flushing is steady to baseline, sample running buffer 5min is not added, then 10 s of sample introduction in a manner of gas driven injection, is powered on Press 10kV operation, cathode ultraviolet (214nm) detection.The result is shown in Figure 1.As can be seen from Figure 1: retention time 10.44,11.90, 13.22,14.19,14.70,16.16, there is peptide peak at 19.38min, and peak shape is independent, no trailing phenomenon illustrates separating effect Well.

2. the determination of polypeptide molecular weight.Take Amersham Biosciences company polypeptide standard items by the above same method Detection, is as a result shown in Fig. 2.Comparison diagram 1,2 can be seen that wherein 10.44,11.90min peptide peak be located at 2512Da or less, illustrate to decompose It is lower than the small-molecular peptides of 2 512Da in object containing molecular weight；13.22,14.19min peptide peak is located at 2 512~6 214 base peaks, Illustrate the peptide for being located at 2 512~6 214Da in decomposition product containing molecular weight；14.70,16.16min peptide peak is located at 6 214~8 159 base peaks illustrate the peptide for being located at 6 214~8 159Da in decomposition product containing molecular weight；19.38min peptide peak is located at 8 159 ~10 700 base peaks, but close to 8 159 base peaks, illustrate the peptide for being greater than 8 159Da in decomposition product containing a small amount of molecular weight.

In addition the polypeptide molecular weight after black fungus soybean protein decomposes concentrates on 2 000~8 000.Divide greatly compared to protein For son amount (100 000~600 000Da [6]), resulting polypeptide is within low molecular weight range.

3. gel permeation chromatography detects

3.1 gel infiltration instrument systems.2414 refractive index detection device (Refractive Index of Waters Detector)；515 geopressure gradients pump；7725i manual injector；Gel chromatographic columns: UltranydrogelTM Linear 7.8 ×300mm column.Chromatographic condition: mobile phase 0.1M NaNO3,35 DEG C of column temperature, flow velocity 0.8ml/min.Standard sample: poly- Molecular weight glycol standard (Ploycethylene gloycol Molecular Weight), place of production Polmer Standards service2USA, range 106,194,440,628,960,1 460,4 290,7 130,12 900,20 600。

The production of 2 standard curves.Standard sample is configured to mobile phase

0.01% solution distinguishes sample introduction, after 0.45 μm of filtering with microporous membrane with the logarithm ln MW couple of standard molecular weight Retention time mapping, can be obtained standard molecular weight working curve, such as Fig. 3.

3 sample tests.By protein,split high purity water soaked overnight, taken after 4 000r/min centrifuge separation Clear liquid, through 0.45 μm of membrane filtration, sample introduction is analyzed after ultrasonic degassing, as a result sees Fig. 4.

By known to Fig. 3,4 controls, 25~40min occur 6 peptide peaks (40min with

Small molecule peak can not integrate out afterwards), it can be obtained with Millennium32 chromatographic work station integral, molecular weight is 12 respectively 826,3 735,1 389,945,481,121Da, and macromolecule molecule content is less, majority has concentrated on small-molecular-weight rank Section, it is similar to capillary electrophoresis detection result.It should be the amino generated in hydrolytic process and with the presence of the molecule of more low molecular weight Sour small molecule.It is heated using multiple, uninterrupted microwave, each 1min, protease will not be made to lose within the shorter time (15min) It is living.

It is heated using multiple, uninterrupted microwave, every time 1

Min will not be such that protease inactivates within the shorter time (15min)

Three, black fungus polypeptide complex function of embodiment is for granule

30 parts of black fungus raw materials, 45 parts of jujube raw materials and 5 parts of ginger raw materials are taken, black fungus is made by by one method of embodiment Polypeptide concentrate and jujube and ginger extract mix well, spray drying.It is with granulator that one gained black fungus of embodiment is small The particle of certain specification is made in molecular activity peptide dry powder, and various quantitative packages required for being packaged into vacuum packing machine are made Granula.

The packaging of granule: 5g/ bags, 20 bags/box.

Black fungus polypeptide complex function is for granule usage and dosage: once in the morning and once at night, 1 bag every time, warm boiled water.

The preparation of example IV black fungus polypeptide complex function oral solution

Take 20 parts of black fungus raw materials, 45 parts of jujube raw materials and 3 parts of ginger raw materials, the concentration of one gained black fungus polypeptide of embodiment Liquid and jujube and ginger extract, which mix well, is sufficiently mixed allotment, emulsification, filling, pasteurization, dispatches from the factory after labeling.It will implement One gained black fungus polypeptide concentrate of example and jujube and ginger extract mix well, and black fungus small-molecular peptides content is in 150mg/ 100ml or more, product soluble solid 3.4g/100g or more.

The preparation of five, black fungus polypeptide complex function oral solution of embodiment

Take 40 parts of black fungus raw materials, 45 parts of jujube raw materials and 8 parts of ginger raw materials, the concentration of one gained black fungus polypeptide of embodiment Liquid and jujube and ginger extract, which mix well, is sufficiently mixed allotment, emulsification, filling, pasteurization, dispatches from the factory after labeling.It will implement One gained black fungus polypeptide concentrate of example and jujube and ginger extract mix well, and black fungus small-molecular peptides content is in 150mg/ 100ml or more, product soluble solid 3.4g/100g or more.

The functional verification of the black fungus polypeptide complex function oral solution of six, example IV of embodiment

1. restoring physical efficiency and mitigating the verification test for moving caused muscle damage

Crowd's grouping: 60 normal adults of validation group, 25~40 one full year of life, male 30, female 30,40 people of control group, 25 ~40 one full year of life, male 20, female 30.

Test method: 60 normal adults of validation group and 40 people of control group participate in the running of 50000 meters of same field respectively, than Pre-games validation group takes the drinks of embodiment, and control group does not take the drinks.After 2 myriametres, verifying race is still able to maintain Former speed is advanced, and it is to slow down that the Athletess speed of control group, which is opened,.Validation group 58 people of agent after end of match damages without muscle Resulting myalgia, swelling feeling are injured, 2 people have the slight swollen feeling of acid；38 people of control group feels severe to moderate flesh Meat pain, swelling.

The result shows that before movement, in movement, supply active peptide can provide sufficient energy to body, be able to suppress flesh The decline of meat strength maintains abundant physical strength for a long time.

2. eliminating the verification test of brain fag

Crowd's grouping: 60 normal adults of validation group, 25~40 one full year of life, male 30, female 30,40 people of control group, 25 ~40 one full year of life, male 20, female 30.

Test method: 60 normal adults of validation group and 40 people of control group are arranged to be involved in reading 4 hours, validation group exists The drinks of embodiment are taken before reading, control group does not take the drinks.After 4 hours read, validation group is detected The rising of the blood oxidation hemoglobin concentration of 40 people of 60 normal adults and control group.As a result, 60 health of validation group at Year, people only had the blood oxidation hemoglobin ratio of 5 people to rise 1~3%, and 40 normal adults of control group have 35 blood oxygen Change hemoglobin ratio and rises 20~30%.

3. the verification test for hindering fat absorption, accelerating lipolysis, promoting basic metabolism

Crowd's grouping: 60 obese adults of validation group, 25~40 one full year of life, male 30, female 30,40 obesities of control group Adult, 25~40 one full year of life, male 20, female 20.

Test method: 60 normal adults of validation group and 40 people of control group are arranged to be involved in and take test, daily The drinks of 200mL embodiment replace lunch, and control group does not take the drinks.After 3 months, 56 people of validation group is fat Figure is significant, and control group does not have change.

4. adjusting the verifying of blood lipid, norcholesterol function

Male mice 60 are taken, is randomly divided into 6 groups by weight, every group 10, respectively Normal group, model group, tool Body embodiment 1-4.

Normal group, feed common distilled water；

Model group, feeding high lipid food (1% cholesterol, 0.5% Pig cholate, 12% lard, basal feed 86.5%) are fed It supports；

Model group gives deep sea fish oil (5mg/kg), twice daily, 2 milliliters every time；

Specific embodiment 1-4 feeds high lipid food (1% cholesterol, 0.5% Pig cholate, 12% lard, basal feed 86.5%) it feeds；

Specific embodiment 1-5 group is fed specific embodiment 1-4 content (5mg/kg), twice daily, 2 milliliters every time；

It is continuous to feed 3 weeks.

Once a day, successive administration 21 days, 1.5h (fasting 12h), mouse pluck eyeball and take blood after the last administration, separate blood Clearly, TG, TC are surveyed with kit method.

Statistical analysis

All experimental datas are indicated with mean ± SD, are examined using t and are carried out statistical analysis, and p < 0.05 thinks there is system Meter learns difference.

Experimental result

Statistical analysis

All experimental datas are indicated with mean ± SD, are examined using t and are carried out statistical analysis, and p < 0.05 thinks there is system Meter learns difference.

Experimental result

Group	TC(mmol/L)	TG(mmol/L)
			Validation group	2.85±0.12	1.28±0.23
Model group	4.23±0.28	2.81±0.21
			Example 1 group	3.90±0.29**	1.91±0.31**
2 groups of embodiment	3.83±0.32*	1.85±0.13*
			3 groups of embodiment	3.82±0.51**	1.62±0.35**
Embodiment 4	3.72±0.30**	1.52±0.22**

^#Normal group of P < 0.01vs；* P < 0.05, * * P < 0.01vs model group

Brief summary:

The content of specific embodiment 1-4 can significantly reduce the content of total cholesterol (TC) and triglycerides (TG), and Sharing for content component has synergistic function in specific embodiment 4.

5. improving the verifying of immunity function to functions of murine peritoneal macrophages/active influence)

Test animal: experimental animal is kunming mice, half male and half female, 18~22 grams of weight.

Experiment is taken to be randomly divided into 4 groups with kunming mice, blank group daily diet, gives tested products by 3 groups of experimental group (composition of embodiment 1-3 preparation), after being administered daily 2 times, 10 days, in 30 minutes after the last administration, (blank group was after feeding 30 minutes), 6% chicken erythrocyte suspension 1.5ml/ of each group mouse peritoneal injection only, extracts mouse peritoneal liquid, sees under microscope Examine, macrophage swallows chicken red blood cell number in the visual field in record 5 minutes, and with formula: phagocytic percentage=swallowed is red thin Erythrocytes × 100% in born of the same parents' number/visual field；Macrophage in the Erythrocytes/visual field for phagocytic index=swallowed Number.Phagocytic percentage is calculated, experimental result see the table below.

The influence of functions of murine peritoneal macrophages

Two groups of curative effects of table compare

Group	Phagocytic percentage	Phagocytic index
			Blank group	25.2±3.89	0.41±0.15
Example 1 group	46.4±5.13	0.62±0.13
			2 groups of embodiment	49.7±4.65	0.69±0.35
3 groups of embodiment	52.9±6.25	0.72±0.23

It can be seen that the mouse of experimental group after drug of the present invention is used continuously from upper table data, the total of macrophage must To enhancing by a small margin, while the activity of macrophage is remarkably enhanced, it is seen that pharmaceutical composition of the invention has good The effect of good resistance building.

6. buffering the functional verification that blood glucose rises

In vivo evaluation

After buying the male SD system big white mouse (CHARLES RIVER company) of 7 weeks sizes, the preparation raising carried out 1 week (is raised Material is subsequently used for testing with MF particle (yeast society of Japan manufactures).

1 obtained active peptide composition (20mg/ml) of embodiment is dissolved in deionized water 1ml, sample solution is made, With feeding tube, the oral big white mouse stomach being sent into after going on a hunger strike 16 hours.After five minutes, saccharic (maltose is orally put by force 2g/kg), it takes a blood sample after 0 point, 30 points, 60 points and 120 minutes from tail vein after saccharic load.By obtained blood at once into Promoting circulation of blood sugar determination (glucose tests PRO, three and chemistry institute).And control group then replaces propolis solution with water.

The effect for inhibiting blood glucose rise is to measure glucose amount in blood according to different periods, compares blood glucose rise area (AUC) judge.Blood glucose rise area calculates according to the following formula:

AUC(h^*Mg/dl)=(a+2b+3c+4d)/4-2a

A, b, c and d in above formula, the blood glucose value after meaning 0 point, 30 points, 60 points and 120 minutes respectively.

Numerical value indicates that effectively poor measurement between 2 groups is examined according to the t of Student and carried out with average value ± standard deviation.

The results are shown in Table 3.N in table indicates sample number.In table and figure^**Indicate with less than 1% level of significance with it is right It is effective poor to compare according to group.

Table 3

With in saccharic load only with the control group of water compared with, 50% methanol of propolis is molten to be had effectively really from component Inhibition blood glucose rise effect, which can effectively continue to saccharic load after sixty minutes, and for AUC, and control group is 130.7±8.3h^*Mg/dl, 50% methanol is molten is divided into 80.7 ± 4.2h from group^*mg/dl.Compared according to the AUC, blood glucose rise suppression Rate processed is 38.3%.

7. active peptide has feritin inhibitory activity and ACE inhibitory activity to the verifying ACE with antihypertensive function simultaneously Inhibitory activity detection method is as follows: 1mL 0.5mM FAPGG (full name N- [3- (2-Furyl) being added in cuvette Acryloyl]-L-phenylalanyl-glycyl-glycine) (it is dissolved in 50mM Tris-HCl buffer, contains 0.3M NaCl, pH 7.5), 20 μ L ACE (1U/mL, final vigor 20mU), (albumen is dense for 200 μ L blood pressure lowering black fungus activity peptide solutions Degree is 1mg/mL；Solvent is 50mM Tris-HCl buffer, NaCl containing 0.3M, pH 7.5), it mixes at room temperature, is surveyed in 2min Determine the reduction rate blank of light absorption value under 345nm and replaces sample to calculate ACE suppression according to the following formula with Tris-HCl buffer System activity:

ACE inhibitory activity (%)=1- [△ A.min^-1 _(sample)/△A.min^-1 _(blank)] × 100%,

Wherein, △ A.min^-1 _(sample)With △ A.min^-1 _(blank)It is the ACE activity suppression rate for indicating sample and blank respectively.

The detection method of feritin inhibitory activity is as follows: blood pressure lowering vegetable seed black fungus active peptide inhibits angiotensins active External test according to renin activity kit (purchase in Cayman Chemical, number 10006270) providing method, press with Lower step measurement: (1) blank: 20 μ L substrates, 160 μ L test buffer, 10 μ L pure water are sequentially added in 96- orifice plate；(2) empty White control: 20 μ L substrates, 150 μ L test buffer, and 10 μ L pure water；(3) sample: 20 μ L substrates, 150 μ L test are used slow Fliud flushing, and 10 μ L samples.The starting reaction of 10 μ L feritins is added in each hole (in addition to blank) respectively later.96- orifice plate room temperature Lower concussion 10s, preheats 15min at 37 DEG C, and the fluorescence intensity of sample, excitation wavelength 340nm, transmitted wave are measured in 10min Long 490nm calculates the feritin inhibitory activity of sample according to the following formula:

Wherein, 100% suppression speed (blank Control) and suppression speed (sample) be the renin activity suppression speed for indicating blank control and sample in 10min respectively.Wherein The test preparation of buffer: (1) buffer: 50mM Tris-HCl buffer, NaCl containing 0.1M, pH 8.0 is prepared；(2) will Blood pressure lowering vegetable seed active peptide is dissolved in the buffer, and the solution that protein concentration is 1mg/mL is made, and buffer is used in as test.

Inhibited using the ACE inhibitory activity 50.84% of the blood pressure lowering vegetable seed black fungus active peptide of above method preparation, feritin Activity 75.31%, has a good application prospect in terms of blood pressure lowering.

8. the functional verification of resisting oxidation and delaying senility

Anti-oxidant experiment:

In order to verify whether product of the invention has oxidation resistant functionality, 1 sample of sample selection example of the present invention Clinical test is carried out, test process is as follows.

100 40~60 years old ages of study subject, health volunteer test.

Detection method:

Experimental group: 50 volunteers are daily test-manufactured by recommendation instructions of taking (daily 2,2 times a day), dose Product；

Control group: in addition 50 volunteers take placebo (vitamin E) as a control group.

Continuously take 30 days.All subjects adhere to primary work during test, and diet is constant.It is tested after test The content that subject takes malonaldehyde, superoxide dismutase and glutathione peroxidase in the blood of front and back is carried out pair Than.

Table 1: the testing result table of MDA, SOD and GSH-Px level in serum

Note: compared with before test-meal,^aP < 0.05；Compared with the control group,^bP < 0.05.

As can be known from the results of Table 1: 50 volunteers sera's malonaldehyde, superoxide dismutase and the paddy Guangs for taking placebo The contents level of sweet peptide peroxidase is without significant change；SOD vigor is significantly raised in test-meal group serum after test, with the same period pair According to relatively, difference is statistically significant (P < 0.05) before group and itself test.In addition, all volunteers during the test and After the test in 6 months, do not occur influencing the reaction of body health.The composition of comprehensive judgement the present embodiment 1 has anti- Oxidative function.

Delay senescence test:

In order to verify the functionality whether product of the invention has anti-aging, sample of the present invention carries out human clinical's survey Examination, test process are as follows:

Skin dimness, coarse 30~50 years old women, 70 people are chosen, is divided into invention group and control group at random, control group is adopted With commercially available common natural vitamin E soft capsule, invention group uses the product composition of embodiment 1.

Application method are as follows: pressure contracting face paper is immersed in clear water and impregnated, and soft capsule is punctured, liquid in soft capsule is instilled In clear water, face paper is impregnated 5 minutes in the mixed liquor of clear water and flexible glue capsule-core liquid then, takes out face paper, opening is applied On the face, face paper is removed after 15 minutes, is cleaned face with warm water, once a night.The shield of other faces used in every group of experimenter Skin condition is same, adheres to that primary work, diet are constant.After 30 days, control group have the facial skin of 18 people relatively using it is preceding it is fine and smooth, Bleach and glossy, invention group have the facial skin of 26 people compared with using it is preceding it is fine and smooth, bleach and glossy.It follows that of the invention Resulting composition can improve skin tone, delay senescence, and effect is significantly better than existing commercially available common natural VE flexible glue Capsule.In addition, all volunteers during the test and after the test in 6 months, do not occur influencing the reaction of body health, It can be using the health care product with preparation for anti-oxidant anti-aging.

9. the functional verification that accelerating alcohol decomposes --- improve for expiration alcohol concentration

Divided 2 times with 6 average age 28 years old male people and tested, each trial interval is greater than 72 hours.Experiment 4: On an empty stomach, 42 degree of white wine 90ml are drunk, drink pure water 300ml before wine.

Experiment 5: on an empty stomach, 42 degree of white wine 90ml is drunk, the formula is drunk before wine and sobers up and drink 300ml.

Experiment 6: on an empty stomach, 42 degree of white wine 90ml are drunk, drink, which is chatted with the black fungus active peptide function one of embodiment with you, before wine is Main Anti-alcohol beverage 300ml.

Conclusion: the formula has improvement result to alcohol breath concentration, i.e., has facilitation to alcohol dehydrogenase activity, Ethanol decomposition efficiency can effectively be promoted.

The formula has excitation alcohol dehydrogenase, aldehyde dehydrogenase activity after can finally drinking by molten water, improve simultaneously Constitution, thus the function of Soboring-up liver-protecting.

10. improving the functional verification of attention

The clinical observation of present composition intervention attention

It collects 20 readme attentions and is difficult to the adult concentrated, the age is between 19-50 years old.Using Shu Er special formula lattice amount Table assesses volunteer's attention, and is divided into 2 groups immediately by attention, and 1 group is treatment group, and 2 groups are control group.Treatment group suffers from The daily edible nourishing of person pastes 30g, wherein the present composition prepared in embodiment 3 ratio in nutrition paste is 16%.Control Group gives the paste of the nutrition without present composition 30g.Nutrition paste is made of red bean, corn etc..After volunteer is 1 month edible again It is secondary that attention force estimation is carried out using scale.As a result such as table 2.

Clinical observation of 2 present composition of table to attention is intervened

Note: compared with the control group: * P < 0.05；* P < 0.01

Experimental result: the edible nutrition paste containing 16% present composition is remarkably improved absent minded crowd Attention scale score, it was demonstrated that the present composition, which has, preferable improves attention effect.

Claims (9)

1. a kind of function edible composition, characterized in that described comprising black fungus polypeptide, Ginger P.E and Fructus Jujubae extract Black fungus polypeptide derives from black fungus raw material, and the Ginger P.E derives from ginger raw material, and the Fructus Jujubae extract derives from Jujube raw material.

2. function edible composition according to claim 1, characterized in that the black fungus raw material, ginger raw material and big The weight ratio of jujube raw material is 20~40 parts: 30~60 parts: 3~10.

3. function edible composition according to claim 1, characterized in that the black fungus polypeptide, Fructus Jujubae extract and The weight ratio of Ginger P.E is 30 parts: 45 parts: 4~6 parts.

4. the preparation method of the described in any item function edible compositions of claims 1 to 3, characterized in that comprising steps of

(1) black fungus small-molecular peptides solution is prepared；

(2) jujube and ginger mixed extract are prepared；

(3) by black fungus small-molecular peptides solution, jujube extracting solution and ginger extract according to 10~20 parts: 30~60 parts: 5~10 Weight ratio mixing preparation；

(4) mixture drying is formed into powder mixture.

(5) using step (4) resulting powder mixture as additive, function edible composition is prepared.

5. the preparation method of function edible composition according to claim 4, characterized in that

(1) prepare black fungus small-molecular peptides solution comprising steps of

Pre-treatment:

A pyrohydrolysis；

B homogenate；

C sterilizing；

Digest black fungus polypeptide:

D is added compound protease and decomposes；

E centrifugation, separates the proteases for decomposing object of black fungus；

(2) prepare jujube and ginger mixed extract include be respectively washed, jujube and ginger, impregnate, decocting, filtering is dense It is reduced to the concentrate that specific gravity is 1.25~1.38；

(3) include by black fungus complex polypeptide concentrate and 10~20 parts: 30 in parts by weight of jujube, ginger concentrate~ 60 parts: 5~10 of parts by weight mix, and spray drying forms mixture dry powder,

Drying in (4) includes that spray drying or reduced vacuum are dry.

6. the preparation method of function edible composition according to claim 5, characterized in that the proteinase complex includes Albumen excision enzyme, protein incision enzyme and food flavor enzyme, the albumen excision enzyme, protein incision enzyme and food flavor enzyme.

7. the preparation method of function edible composition according to claim 5, characterized in that the proteinase complex is by wood Melon restriction endonuclease and yeast flavor enzyme composition.

8. the preparation method of function edible composition according to claim 7, characterized in that the proteinase complex is by egg Ultrawhite enzyme cutting and protein incision enzyme composition, the albumen excision enzyme, protein incision enzyme composition ratio are 1:10~10:11 or excellent Select 1:5`5:1, or more preferably 1:2~2:1.

9. function edible composition according to claims 1 to 8, characterized in that the function edible composition include but It is not limited to capsule, lozenge, drink with function and biscuit.