KR20110118911A - Lactic acid bacteria fermented extract using cereals and herb medicine materials, and method of preparing thereof - Google Patents

Abstract

PURPOSE: A fermented grain extract using medical herbs and lactic acid bacteria and a producing method thereof are provided to prepare high protein low calorie meal alternative or dietary food, which has an antioxidant effect by removing active oxygen and activates lactic acid bacteria to enhance the degree of absorption of nutrients. CONSTITUTION: A producing method of a fermented grain extract using medical herbs and lactic acid bacteria comprises the following steps: mixing 100 parts of hydrogen-rich water by weight and 0.5-10 parts of glycyrrhizae radix, astragalus membranaceus, angelica sinensis, or their mixture by weight; double boiling the mixture with water at 80-100 deg C for 5-6 hours; inserting 25-35 parts of grain by weight selected from soybeans, red beans, mung beans, brown rice, barley, corn, and other grains into the medical herb mixture; grinding the mixture before filtering grain juice; heating the filtered grain juice to 97-105 deg C, and filtering to obtain a lactic acid bacteria culture medium; injecting the lactic acid bacteria to the medium, and fermenting at 35-37 deg C for 20-30 hours; and extracting amino acid from the aged product.

Description

Lactic Acid Bacteria Fermented Extract Using Cereals and Herb Medicine Materials, and Method of Preparing Thereof}

The present invention relates to lactic acid bacteria grain fermented extract using a herbal medicine and a method for producing the same. More specifically, by fermenting herbal extracts and grains with lactic acid bacteria using hydrogen rich water, it removes free radicals when ingested, promotes the absorption of minerals in the body, promotes the proliferation of lactic acid bacteria, enhances the intestinal immune function, and harmful bacteria. And it relates to a high protein low calorie lactic acid bacteria fermented extract that can supplement the diet with the effect of removing and inhibiting the antigen and a preparation method thereof.

The human digestive system is approximately 9-10 meters from the mouth to the anus. The ingested food is passed through the esophagus and is made in the stomach from the stomach to the intestine. During intestinal digestion, food stays in the body for 30-120 hours depending on the intestinal environmental conditions. The optimal intestinal environmental condition is digestion in the range of 30 hours due to the proliferation of enteric beneficial bacteria. As a result, some of the ingested food is reduced to nutrients and absorbed into the nutrients of the body through the villi of the small intestine, and the remainder is discharged through the anus as the residue left by microorganisms. On the other hand, when the intestinal beneficial bacteria are not proliferated and the digestion period is over 30 hours, abnormal fermentation occurs due to the temperature of 36 ° C. or higher, and ammonia gas and active oxygen are generated by the harmful intestinal bacteria.

Among the microorganisms that provide nutrition to the cells that make up the human body, 20% of the beneficial bacteria, 30% harmful bacteria, and 50% opportunistic bacteria, they live together while living together. In healthy people, 50% of opportunistic bacteria produce beneficial substances, but in others, 50% of opportunistic bacteria produce harmful metabolites, which can lead to diseases such as various adult diseases, obesity, atopy, diabetes, high blood pressure, and cancer. Among the microorganisms in the intestine, whether opportunistic bacteria act as beneficial or harmful bacteria is an important measure of human health.

Lactic acid bacteria have physiological activities such as inhibition of proliferation of harmful bacteria, alleviation of lactose intolerance, lowering of serum cholesterol, anticancer activity, immunity, diarrhea and constipation improvement, and vitamin synthesis. In addition, lactic acid fermentation prevents food spoilage, secretes antibacterial substances such as bacteriocins to suppress food poisoning bacteria, and lowers intestinal hydrogen ion concentration (pH) in humans to inhibit the growth of intestinal rot bacteria. Acts as.

It is a diet substitute for the purpose of losing weight or losing weight of modern people with busy and irregular dietary habits. It uses various grains such as soybeans and various high-proteins containing nutrients such as amino acids, vitamins, minerals, lipids, and dietary fiber that the human body needs. Low calorie foods are available.

The rich nutrients and bioactive substances in grains are absorbed by 30-50% when cooked and eaten raw, whereas the fermentation of grains by lactic acid bacteria shows more than 90% absorption. In recent years, by adding a fermentation process to these ingredients, it is more diverse than the grain itself and contains a large amount of nutrients and physiologically active substances that are easily absorbed by the human body, thereby activating the human beneficial bacteria to the intestine.

As an example, Korean Patent Publication No. 2001-0069777 discloses odorless fermented soybeans by inoculating Bacillus Bacillus subtilis CHO99 (Accession No. KCTC-18052P) to soybeans for 12 to 36 hours to produce odorless fermented soybeans, It describes a healthy functional fermented soybean prepared by adding acidulant, flavoring, etc. to dry it to a moisture content of 8 to 40% at room temperature. Powdered and mixed with flour to prepare a new type of functional food.

As another example, Korean Patent Application No. 2008-93084 discloses a first step of obtaining red ginseng extract by mixing ocher Jijangsu and red ginseng powder and then heating it; A second step of making red porridge by mixing soybean powder with red ginseng extract; Adding a sugar to the porridge and inoculating the lactic acid bacteria to ferment to make fermented porridge; The fourth step of mixing the fermented porridge mixed grain powder fermentation and fermentation and fermentation, and the method for producing a fermented meal of lactic acid bacteria grains comprising red ginseng powder, comprising a, as a health food It is fermented with red ginseng at the top, soybean powder, which is a source of vegetable protein as a whole food, and lactic acid bacteria that balance vitamins and minerals with grains of carbohydrates and improve the intestinal environment.

In addition, research on a variety of ways to increase the absorption of food in the body by further activating the intestinal lactic acid bacteria using herbal medicines.

 It is an object of the present invention to inoculate and ferment lactic acid bacteria using a herb extract which promotes the growth of lactic acid bacteria in grains such as soybeans, and by using hydrogen-rich water as the water used in the preparation of extracts, the removal of active oxygen and absorption of minerals in the body To promote, and to promote the proliferation of lactic acid bacteria to enhance the immune function of the intestine, and to provide a high protein low calorie fermented extract of lactic acid bacteria fermentation extract and a method for producing the same can be supplemented with harmful bacteria and antigen removal and suppression efficacy.

Lactic acid bacteria grain fermentation extract according to the present invention, the herb extract, soybean, red beans, green beans, brown rice, oats, barley, rye, corn, licorice, Astragalus, Angelica, or a mixture of two or more thereof, hydrothermally extracted using hydrogen-rich water Extracted by fermenting one or more grains from crude, sorghum and millet with Bifidobacterium, Lactobacillus acidophilus and Lactococcus Lactis, or Lactobacillus It is characterized by being manufactured.

In addition, the production method of the lactic acid bacteria grain fermentation extract according to the present invention, after mixing 0.5-10 parts by weight of licorice, Astragalus, Angelica or two or more mixtures thereof with respect to 100 parts by weight of hydrogen-rich water at a temperature of 80-100 ℃ A first step of preparing an herbal extract by boiling water for 5-6 hours; 25-35 parts by weight of one or more grains of soybean, red beans, green beans, brown rice, oats, barley, rye, corn, crude, sorghum and millet with respect to 100 parts by weight of the hydrogen-rich water mixed with the herbal medicine extract, and then immersed Filtering the grain juice obtained by grinding and then heating at a temperature of 97-105 ° C., or heating the grain juice at the same temperature range and then filtering to form a lactic acid bacteria culture medium; Inoculating the medium with lactic acid bacteria, and then incubating, fermenting and maturing for 20-30 hours at a temperature of 35-37 ° C. in a culture tank; And a fourth step of extracting only amino acids from the aged material.

Hydrogen-rich water is used as water used for preparation of the extract in the present invention. 'Hydrogen-rich water' means water containing 200-500 times more hydrogen than ordinary water (hydrogen-deficient water), which is fast absorbed by the body because the water molecules are much smaller than ordinary water and is active. It contains a large amount of hydrogen, which combines with active oxygen in the body to remove the active oxygen, which has an antioxidant function, promotes the absorption rate of minerals in the body, and has the effect of enhancing immune function. Hydrogen-rich water in the present invention can be used without limitation as long as the content of hydrogen does not deviate from the above range and purchased on the market.

Licorice (甘草; Glycyrrhiza uralensis Fisher et. De candolle) is a perennial herb, a perennial herb belonging to the legume family, and tastes sweet and nontoxic. Eliminates poisoning, spleen and comfort inside, clear and detoxification (解毒) effect. In Dongbogam and others, 'the quality is flat, the taste is sweet, and there is no poison. It relieves all kinds of medicines. It is called Guk-ro because it has the effect of harmonizing all medicines such as 72 mineral medicines and 1,200 herbal medicines. There is a heat of fraud [5] in chapter 5, and it is written, and it is made through 9 gyu (竅), and all the blood vein is good. It also strengthens and strengthens tendons and bones. In February and August of the lunar calendar, the roots are dug up and dried in the sun. When you break the powder comes out because it is called Buncho (본 草). Licorice enters the foot ginseng penis (足 三陰 經), and when baked, the stomach harmonizes, and when used raw, fire (瀉) [탕 액]. People who vomit, are full, or drink alcohol are not good to eat for a long time or to eat a lot [blackout]. It looked in from China and planted in various parts of Korea, but did not breed well. However, it was best to fly in North Hamgyong Province. Licorice has a natural antibiotic action, promotes the growth of lactic acid bacteria, eliminates and suppresses harmful bacteria and antigens, and also has the function of protecting the health of the intestine by enhancing the immunity of the intestine. Glycyrrin, a component of licorice, is converted into 18beta-glycyrrhetic acid (GA) by intestinal bacteria and absorbed into the blood. It is known that the recovery of bioactivity of glycyrazine is accelerated.

Astragalus (黄 朞) is sweet and slightly warm, mainly acting on the spleen and lungs. Bogi boosts the energy and lifts the nourishment, high labeling to prevent sweating by firming the epidermis, and turbid poisoning to treat boils on the skin.膿) action, smooth water metabolism to treat edema by treating edema (利 水 退 腫) is also used in many diseases of children. Due to the weakness of the spleen, it is used for the appearance of white or yellowish face, limb boredom, and faint stool. In addition, the weakness and weakness of the hematopoietic organs due to weakness, weakness and paleness of the face is pale, there is no gloss, it is used when symptoms such as hemorrhage, hemostasis, subcutaneous bleeding, uterine bleeding. Efficacy in raising energy, so it is effective for symptoms such as sewage, prolapse, long-term dehydration, and declining energy. It is also used for the symptoms of yellowish, dizzy, lack of energy, difficulty speaking, cold sweating, heartbeat and inability to sleep. In addition, it is known that it is used when the skin rash is not healed for a long time, and it is known to be used for skin paralysis and sensory paralysis due to blood circulation disorder without the symptoms and energy that cannot be excreted due to excessive moisture stagnation in the body.

In the above, Angelica (當歸) to clear the bad blood, blood acts. In Dongbogam, 'the quality is warm [溫], the taste is sweet, very [甘辛], there is no poison. Heals all wind sickness, blood clots, and frailty, heals inclement blood, and gives rise to new blood. It is mainly used for the impregnation and wife's disintegration and inability to conceive, and heals various bad spears and irons to heal the blood. This dysentery swells the stomach, heals the whole body, saves chapter 5, and revives the flesh. It is also planted in the mountains and fields. In February and August of the lunar calendar, roots are dug up and dried in the shade. It is best to be fat and not thin. Or it is good to have a lot of flesh, to be damp and not dry. Or horsetails are good. When trying to get rid of the blood, write a hard word on the head of the head, and when you want to stop the pain or stop the bleeding, use the roots. If you use a head, you can bleed through the blood. If you write the whole, you will be bleeding on the one hand and stopping on the one hand, ie evening the blood. It enters the hydrogen penis because the mind presides over blood. It also enters the pedigree penis because the rain leads the blood. It also enters the foot penis because it stores blood [liquid juice]. When the blood donation (혼란) is confused, eat it soon. It has the effect of bringing it to the appropriate place, so if you want to heal your body disease, soak it in alcohol and write it.If you want to cure the outside bottle, wash it with alcohol and write it. Stir-fry it in [炒] and write [Introduction]. It is good to soak in alcohol [mobilization]. '

In the lactic acid bacteria grain fermentation extract according to the present invention, the licorice, Astragalus, Angelica, or a mixture of two or more thereof, based on 100 parts by weight of hydrogen-rich water, is used in an amount of 0.5-10 parts by weight. If it is out of this range, it is impossible to prepare an extract having a suitable lactic acid bacteria growth promotion, efficacy to suppress elimination of harmful bacteria and antigens.

These herbal medicines used in the present invention can be used without limitation so long as they have the effect of promoting lactic acid bacteria growth, these can be used without limitation if purchased in the market.

As the grains used in the present invention, one or more grains of soybean, red beans, green beans, brown rice, oats, barley, rye, corn, crude, sorghum and millet may be used, and may be ground in accordance with conventional methods in the art. Can be used. Instead of soybeans, a mixture of beans and soybeans, which can be added to conventional grain rice, such as peas, kidney beans, chungtae, black beans, and frost tea, may also be used. This single grain or a mixture thereof is used in an amount of 25 to 35 parts by weight based on 100 parts by weight of the hydrogen rich number. If it is out of this range, it is impossible to prepare an extract having a suitable lactic acid bacteria growth promotion, efficacy to suppress elimination of harmful bacteria and antigens. By adjusting the blending ratio for each of these grains in the total amount of grain used, the nutritional ratio, aesthetics, color and the like of the extract produced can be variously changed. More preferably, it is used to mix soybean and one or more kinds of other grains except soybean, and 10-15 parts by weight of soybean, 10-15 parts by weight of brown rice and the remaining amount, based on a total of 25-35 parts by weight of the grain mixture. As a grain mixture containing red beans, green beans or mixtures thereof are used.

As the lactic acid bacteria used in the present invention, only Lactobacillus bacteria or mixed bacteria of Bifidobacterium, Lactobacillus acidophilus and Lactococcus Lactis are used. Most preferably, only Lactobacillus bacteria are used. The lactic acid bacteria is used in an amount of 3-5 parts by weight based on 100 parts by weight of the hydrogen rich number. If it is out of this range, it is impossible to prepare an extract having a suitable lactic acid bacteria growth promotion, efficacy to suppress elimination of harmful bacteria and antigens.

The hydrogen rich number; Licorice, Astragalus, Angelica or mixtures of two or more thereof; One or more grains of soybean, red beans, green beans, brown rice, oats, barley, rye, corn, crude, sorghum, millet; And using lactic acid bacteria, in the production of lactic acid bacteria grain fermented extract, the first step of producing a herbal extract; Forming a lactic acid bacteria culture medium; Inoculating the lactic acid bacteria, the third step of culturing, fermenting and aging; The fourth step is to extract only amino acids.

In the first step, 0.5-10 parts by weight of licorice, astragalus, Angelica or two or more kinds thereof are mixed with 100 parts by weight of the hydrogen-rich water, and heated at a temperature of 80-100 ° C. for 5-6 hours. do. In this process, the herbal ingredients are extracted while being dissolved in hydrogen-rich water, and the weakness is easily absorbed. The hot water must be used to prevent the heat from directly affecting the herbal or hydrogen rich water so as not to destroy or alter various nutrients and pharmacological components including minerals.

The second step, the herbal medicine obtained 25-35 parts by weight of one or more grains of soybean, red beans, green beans, brown rice, oats, barley, rye, corn, crude, sorghum, millet with respect to 100 parts by weight of the hydrogen-rich water After mixing with the extract, it was immersed in the water soaking tank to facilitate the extraction of nutrients such as vegetable protein, carbohydrates, vitamins, minerals, and dietary fiber in the grain, and then the grain juice (soybean juice) concentration in the milling period is 7 ~ 12 brix Grain the grains while soaking the water 5-8 times as much as possible to obtain grain juice (soybean juice). The grain juice (soybean juice) is separated from soybean juice and soybeans in a filter and heated to 97-105 ° C in a heating pot, or the soybean juice is heated to 97-105 ° C in a heating pot so that the soybean juice and soybeans are Isolate to get soymilk.

In the third step, after inoculating 3-5 parts by weight of the lactic acid bacteria with respect to 100 parts by weight of the hydrogen-rich water in the medium (soy milk), incubation at a temperature of 35-37 ℃ in culture tank for 20-30 hours, fermentation and Mature. Most preferably, the temperature is 36.5 ° C. At this time, it has a floating yogurt (hosang) formulation in the form of soft tofu. It is possible to produce floating soy yogurt by further mixing 2-10% by weight fructose and 5-20% by weight of fruit syrup with respect to 100% by weight of the yogurt in this arc.

In the fourth step, the lactic acid bacteria are centrifuged according to a conventional method and only amino acids are extracted. Then, sterilization and sterilization is completed by storing for 30 minutes at a temperature of 100 ℃, then packed in a container in a predetermined dosage unit.

In addition, conventional sweeteners, acidulants, flavorings and the like used in fermentation products may be added according to various tastes.

After undergoing the amino acid extraction process, only the lactic acid bacteria collected and dried at a low temperature vacuum drying method at 70 ℃ temperature may be further powdered or granulated in a granulator according to a conventional method may be further added. Due to this, the lactic acid bacteria remain dormant, and when the lactic acid bacteria can adapt to the environment, they can be actively reactivated.

According to the present invention, it is possible to provide a high-protein, low-calorie meal-substituted fermentation formula containing nutrients such as proteins, vitamins, minerals, lipids, and dietary fiber that the human body needs to those who are easily skipped meals due to busy and irregular life, It can provide a diet fermentation formula that provides balanced nutrients to those who want to reduce weight, and also its antioxidant function by removing free radicals in the body when it is ingested, further activate the intestinal lactic acid bacteria, remove harmful bacteria and antigens Alternatively, by suppressing, the absorption rate of the food in the body can be increased.

Hereinafter, preferred embodiments of the present invention will be described. The following examples are intended to illustrate the invention and do not limit the scope of the invention.

Example  1-Preparation of Fermented Extract

5 parts by weight of licorice powder was mixed with 100 parts by weight of hydrogen-rich water, followed by heating at a temperature of 90 ° C. for 6 hours in water. After mixing 30 parts by weight of a 6: 6: 1: 1 mixture of soybean, brown rice, red beans, and mung beans with 100 parts by weight of the hydrogen-rich water in a bath solution according to a conventional method, Grain crushed grains were obtained by hydrolyzing 5-8 times the purified water so that the concentration of grain juice was 7-12 brix in the wedge. This soybean juice and soybeans were separated by a filter, and heated to 97-105 ° C. in a common heating cooker to obtain a soymilk medium. Thereafter, the medium was cooled, and then inoculated with 5 parts by weight of Lactobacillus bacteria with respect to 100 parts by weight of the hydrogen-rich water, and then cultured, fermented and aged at a temperature of 36.5 ° C. for 30 hours in a culture tank. Then, the lactic acid bacteria were centrifuged according to a conventional method, followed by extraction of only amino acids, sterilized and sterilized, stored at 100 ° C. for 30 minutes, and then packaged in a 100 ml container.

Example  2-Preparation of Fermented Extract

In Example 1, a 6: 6: 1: 1: 1: 1: 1: 1 mixture of soybean, brown rice, red beans, green beans, oats, barley, rye, and corn as a grain mixture was used, and bifidus instead of Lactobacillus bacteria was used. Fermented extracts were prepared according to the same method except for using a 1: 1: 1 mixed bacterium of the bacterium, Ecidophilus and Lactococcus lactis.

Example  3-Preparation of Fermented Extract in Powder Formulation

After the amino acid extraction in Example 1, only pure lactic acid bacteria were collected and dried at a temperature of 70 ℃ in a low temperature vacuum drying method and then powdered according to a conventional method to prepare a fermentation extract of powder formulation.

Example  4-preparation of fermented extract of granule formulation

After the amino acid extraction process in Example 1, only the lactic acid bacteria were collected, dried at a low temperature vacuum drying method at 70 ℃ temperature and granulated in a granulator according to a conventional method to prepare a fermentation extract of the granule formulation.

Test Example  One - Example  Component analysis of fermented extracts according to 1

The 100 ml extract prepared in Example 1 was subjected to the Japan Food Analysis Center (09037604001-02) and subjected to lactic acid bacteria count, calorie content and component analysis. As a result, the number of lactic acid bacteria is more than 1 billion, the calorie is 357 kcal, the results of the component analysis is shown in Table 1 below.

amino acid
(Unit: mg) vitamin mineral
(Unit: mg) fatty acid
(unit: %) Arginine 264 Riboflavin B2 0.11mg sign 76.9 16: 0 10.3 Lee Sin 253 Vitamin B6 62 µg iron 1.80 16: 1 0.3 Histidine 99 Vitamin c 0.2mg calcium 16.3 17: 1 1.0 Phenylalanine 184 β-tocophenol 0.1mg potassium 161 18: 0 3.5 Tyrosine 142 γ-tocophenol 1.3mg magnesium 22.9 18: 1 17.1 Leucine 292 δ-tocophenol 0.7mg Copper 0.10 18: 2n-6 52.8 Isoleucine 156 Folic acid 50 µg zinc 0.28 18: 3n-3 12.4 Methionine 59 Pantothenic Acid 0.08mg manganese 0.32 20: 0 0.3 Balin 186 Biotin 8.7 µg Selenium 5 µg 22: 0 0.5 Alanine 202 Niacin 1.61mg molybdenum 0.1 24: 0 0.2 Glycine 178 Tryptophan 57mg Unidentified 1.6 Proline 200 Other vitamins 1.3 g Other fatty acids 114mg Glutamic acid 631 Serine 181 Threonine 158 Aspartic acid 410 Tryptophan 57 Cystine 49 Saponin 37

As can be seen in Table 1, the fermented extract according to the present invention is configured to contain a variety of essential amino acids, vitamins, minerals and fatty acids in abundant amounts, it is suitable as a nutritional replacement diet or diet of high protein low calorie.

Claims (9)

At least one of Chinese herb extract, soybean, red bean, green bean, brown rice, oat, barley, rye, corn, crude, millet, millet A fermented extract of lactic acid bacteria grain fermented with herbal medicines prepared by fermenting grains of Bifidobacterium, Lactobacillus acidophilus and Lactococcus Lactis mixed or fermented with Lactobacillus. The method according to claim 1, wherein the medicinal herb or mixture thereof is used in an amount of 0.5-10 parts by weight, and the grains are used in an amount of 25-35 parts by weight, based on 100 parts by weight of hydrogen-rich water used in hot water extraction. Lactic acid bacteria Lactic acid bacteria grain fermented extract using herbal medicine, characterized in that used in an amount of 3-5 parts by weight. According to claim 1, The grain is lactic acid bacteria grain fermentation extract using herbal medicine, characterized in that the mixture of soybeans and one or more grains of other grains except soybeans. According to claim 1 or claim 2, wherein the hydrogen rich water is lactic acid bacteria grain fermented extract using herbal medicine, characterized in that the water content of hydrogen is approximately 200-500 times compared to ordinary water. According to claim 1, The extract is lactic acid bacteria grain fermentation extract using herbal medicine, characterized in that having a formulation of staple yogurt, powder or granules. The first step of preparing a herbal medicine extract by mixing 0.5-10 parts by weight of licorice, Astragalus, Angelica or two or more mixtures thereof with respect to 100 parts by weight of hydrogen-rich water and then in water for 5-6 hours at a temperature of 80-100 ° C. step;
25-35 parts by weight of one or more grains of soybean, red beans, green beans, brown rice, oats, barley, rye, corn, crude, sorghum and millet with respect to 100 parts by weight of the hydrogen-rich water mixed with the herbal medicine extract, and then immersed Filtering the grain juice obtained by grinding and then heating at a temperature of 97-105 ° C. or heating the grain juice at the same temperature range and then filtering to form a lactic acid bacteria culture medium;
A third step of inoculating the medium with 100 parts by weight of the hydrogen-rich water, followed by incubation, fermentation and aging for 20-30 hours at a temperature of 35-37 ° C. in a culture tank; And
Method for producing a lactic acid bacteria grain fermentation extract using herbal medicine, characterized in that the fourth step of extracting only the amino acid of the aged material. The method of claim 6, wherein the hydrogen-rich water is a method of producing lactic acid bacteria grain fermented extract using herbal medicine, characterized in that the water content of hydrogen is approximately 200-500 times compared to ordinary water. The lactic acid bacterium is a mixed bacterium or a Lactobacillus bacterium of Bifidus bacillus, Escidophilus and Lactococcus lactis, and is used in an amount of 3-5 parts by weight based on 100 parts by weight of hydrogen-rich water. Method of producing lactic acid bacteria grain fermented extract using the herbal medicine. The method of claim 6, further comprising the step of drying the cultured lactic acid bacteria after extraction of the amino acid under low temperature vacuum powder or granulate.