CN116898098A - Boletus peptide anti-alcoholic formula and raw material preparation method - Google Patents

Boletus peptide anti-alcoholic formula and raw material preparation method Download PDF

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Publication number
CN116898098A
CN116898098A CN202310897236.9A CN202310897236A CN116898098A CN 116898098 A CN116898098 A CN 116898098A CN 202310897236 A CN202310897236 A CN 202310897236A CN 116898098 A CN116898098 A CN 116898098A
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parts
liver
lemon
powder
extract
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马海增
张贵士
司云鹏
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Zhongke Kangsheng Hebei Biotechnology Co ltd
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Zhongke Kangsheng Hebei Biotechnology Co ltd
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A23L33/18—Peptides; Protein hydrolysates
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Nutrition Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Botany (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

The invention discloses a bovine liver peptide anti-alcohol formula and a raw material preparation method, wherein the formula comprises the following raw materials: beef liver, corn gluten meal, kudzuvine root, hovenia dulcis thunb, chrysanthemum and lemon. The beneficial effects are that: the invention not only can achieve the effect of protecting the liver through the active bovine liver peptide (namely glutathione) extracted from the bovine liver, but also can add the medicinal and edible Chinese herbal medicines with the same source of medicine and food for dispelling the effects of alcohol and protecting the liver through the monarch, minister, assistant and guide compatibility principle, and the formula has the effects of sobering up and protecting the liver, soothing the nerves and improving eyesight, clearing heat and detoxicating, and promoting diuresis and treating stranguria.

Description

Boletus peptide anti-alcoholic formula and raw material preparation method
Technical Field
The invention belongs to the field of nutritional foods, and particularly relates to a beef liver peptide anti-alcohol formula and a raw material preparation method.
Background
The liver is an organ in the body that is mainly metabolic functions and plays a role in deoxidation, storage of hepatic glucose, synthesis of secreted proteins, and the like in the body. The liver also produces bile in the digestive system. The liver is the largest organ in the viscera of the human body, located in the abdominal position in the human body, under the right diaphragm, at the front end of the gallbladder and in front of the right kidney, above the stomach. The liver is the largest digestive gland in the digestive system of human body and is a reddish brown V-shaped organ. The liver is also an important organ of metabolism. Liver is a fragile organ, and after invasion of pathogenic factors such as viruses and the like into the liver, the liver can be caused to have normal function decline, most liver diseases can be cured, but a small number of liver diseases are converted into chronic hepatitis.
Liver injury can be classified into chemical liver injury, pathological liver injury and violent liver injury according to etiology. Wherein, the violent liver injury is obviously rapid and serious; pathological liver injury and chemical liver injury are the most common.
Chemical liver injury is mainly caused by chemical drug poisoning, allergy and the like, and can cause liver injury or liver function abnormality such as drug hepatitis, alcoholic liver, organic pesticide poisoning and the like. However, the most common and rising drug-induced liver injury is caused, and with the continuous development of new drugs, the liver toxicity of drugs is a difficult problem, and it is reported that recent thousands of drugs can cause liver injury, the development of biological technology will reduce the risk of certain liver toxicity of drugs, the key factor of alcoholic liver disease is alcohol, the alcohol oxidation causes liver and other organ injury, and the reduction of drinking volume and even abstinence from alcohol are the basis of treatment.
Glutathione is clinically used as an important pharmacological component for protecting liver, and currently, glutathione tablets or additives of medicines are clinically used for protecting liver, so that the glutathione is widely used for treating alcoholic liver injury, primary liver cancer, viral hepatitis and the like, has good curative effects, can improve liver microcirculation by increasing the content of nitric oxide in liver tissues, has important roles in animals and plants, and has high content in animal livers of 100-1000 mg/100g;
however, the administration of glutathione alone is easy to cause the disadvantage of inadequacy, and the effect of glutathione alone cannot be fully stimulated, so that the effect after administration is poor.
Disclosure of Invention
The invention aims to provide a beef liver peptide anti-alcoholic formula and a raw material preparation method for solving the problem of avoiding alcoholic liver injury after drinking.
In order to achieve the technical purpose, the invention provides the following technical scheme:
the invention provides a bovine liver peptide anti-alcohol formula which is prepared from the following raw materials: beef liver, corn gluten meal, kudzuvine root, hovenia dulcis thunb, chrysanthemum and lemon.
Preferably, the material comprises the following raw materials in parts by weight: 30-35 parts of beef liver, 20-25 parts of corn gluten meal, 15-20 parts of kudzuvine root, 10-15 parts of hovenia dulcis thunb, 5-10 parts of chrysanthemum and 1-5 parts of lemon.
Preferably, the material comprises the following raw materials in parts by weight: 35 parts of beef liver, 25 parts of corn gluten meal, 15 parts of kudzuvine root, 10 parts of hovenia dulcis thunb, 5 parts of chrysanthemum and 5 parts of lemon.
The invention also provides a formula for preparing the bovine liver peptide for dispelling the effects of alcohol and a preparation method of raw materials, which are characterized in that 35 parts of bovine liver, 25 parts of corn gluten meal, 15 parts of kudzuvine root, 10 parts of hovenia dulcis thunb, 5 parts of chrysanthemum and 5 parts of lemon are respectively weighed according to the weight parts of the bovine liver, the corn gluten meal, the kudzuvine root, the hovenia dulcis thunb, the chrysanthemum and the lemon, and the bovine liver peptide, the corn oligopeptide powder, the kudzuvine root extract, the hovenia dulcis thunb extract, the chrysanthemum extract and the lemon powder are respectively prepared by the following extraction methods of the 6 extracts:
bovine liver peptide powder: mechanically removing the outer fascia of the beef liver, cutting into 5 x 5cm blocks, soaking and cleaning, homogenizing by a colloid mill, and extracting the homogenized feed liquid by enzymolysis: adding 2 times of water, heating the raw material liquid to 55 ℃, adding animal protease (2 ten thousand U/g) with the concentration of 1% relative to the substrate, and carrying out enzymolysis for 4 hours; after enzymolysis, carrying out enzyme deactivation treatment on the raw material liquid at 90 ℃ for 30min, adding activated clay for adsorption at the same time of enzyme deactivation, wherein the adsorption time is 30min, and the adding amount is 2% of the volume of the feed liquid; the enzyme deactivation adsorption adopts a horizontal centrifuge for centrifugation, the discharge slag and the activated clay are separated, and the supernatant fluid is taken; purifying with 10kDa ultrafilter membrane; concentrating the purified feed liquid to 40% by double effect, and spray drying to obtain liver peptide powder;
corn oligopeptide powder: after the corn protein powder is dissolved by adding water, the feed liquid ratio is 1:3, heating to 45 ℃, adding alkaline protease (1 kiloU/g) and papain (2 kiloU/g) with the concentration of 0.5% relative to the substrate, carrying out enzymolysis for 3 hours, carrying out enzyme deactivation treatment on raw material liquid at 90 ℃ for 30 minutes after enzymolysis, carrying out tubular separation at 14000rpm after enzyme deactivation is finished, taking supernatant, and purifying by a 1kDa ultrafiltration membrane; concentrating the purified feed liquid to 40% by double effect, and spray drying to obtain corn oligopeptide powder;
radix Puerariae extract: pulverizing radix Puerariae with 80 mesh, adding 10 times of water, leaching with 90 deg.C water for 3 hr, continuously leaching for 3 times, centrifuging, collecting supernatant, concentrating to 1/5 of the total volume with double effect concentration, and spray drying to obtain radix Puerariae extract;
semen Hoveniae extract: pulverizing semen Hoveniae into coarse powder, and leaching with 10 times of water at 70deg.C for 2-3 hr; centrifuging, collecting supernatant, concentrating with double effect concentration to 1/5 of the total volume, and spray drying to obtain semen Hoveniae extract;
flos Chrysanthemi extract: crushing chrysanthemum into coarse powder, and leaching with 10 times of water at 70 ℃ for 2-3 h; centrifuging, collecting supernatant, concentrating by double effect concentration to 1/5 of the total volume, and spray drying to obtain flos Chrysanthemi extract;
lemon powder: cleaning lemon, peeling, removing pulp and seeds, crushing, squeezing, centrifuging at 14000rpm to obtain clear lemon juice, adjusting pH to 4.5-5.5 with sodium bicarbonate, adding starch for molecular embedding, and spray drying to obtain raw lemon pulp powder.
The efficacy of the formula raw materials in the invention is as follows:
bovine liver: regulating liver function; promoting metabolism; improving the antitoxic function of liver, enhancing immunity, and regulating bile production and biliary tract tension; protecting liver and resisting toxic materials.
Corn oligopeptide powder: corn oligopeptides are an angiotensin converting enzyme inhibitor which achieves a blood pressure lowering effect by inhibiting the activity of angiotensin converting enzyme. Has antioxidant effect similar to SOD (superoxide dismutase), has strong scavenging effect on superoxide anion free radical and hydroxyl free radical, and has unique amino acid composition, which is beneficial to promoting alcohol metabolism.
Radix Puerariae extract: sobering up, reducing blood sugar, heart failure, hypertension and myocardial infarction, and is clinically used for treating cardiovascular and cerebrovascular diseases, cancers, parkinsonism, alzheimer's disease, diabetes, diabetic complications and the like.
Semen Hoveniae extract: sobering up, tranquillizing, dispelling wind and dampness, is rich in loline and hovenin, and has the pharmacological effects of protecting liver, dispelling alcohol, resisting tumor, preventing and treating cancer, etc.
Flos Chrysanthemi extract: dispelling pathogenic wind, clearing heat, suppressing hyperactive liver, improving eyesight, lowering blood pressure, and is rich in chlorogenic acid, reducing body fat, resisting oxidation and aging, regulating blood sugar, resisting bacteria, relieving inflammation, resisting virus, etc.
Lemon powder: the lemon can remove fishy smell, is rich in organic acid, and can interact with ethanol to form esters, thereby achieving the purpose of dispelling effects of alcohol.
The beneficial effects are that: the invention takes bovine liver peptide (glutathione) as a monarch, corn oligopeptide powder as a minister, kudzuvine root extract, hovenia dulcis thunb extract and chrysanthemum extract as an assistant, and lemon powder as a guide. So as to achieve the effects of dispelling the effects of alcohol, protecting liver, soothing the nerves, improving eyesight and the like, ensure the edible safety and effectiveness by reasonable collocation of homology of medicine and food, avoid the effect of singly supplementing a certain kind of medicine, and avoid the phenomenon of hesitation.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be described in detail below. It will be apparent that the described embodiments are only some, but not all, embodiments of the invention. All other embodiments, based on the examples herein, which are within the scope of the invention as defined by the claims, will be within the scope of the invention as defined by the claims.
Embodiment one:
the bovine liver peptide anti-alcoholic formula consists of the following raw materials in parts by weight: 30 parts of beef liver, 20 parts of corn gluten meal, 15 parts of kudzuvine root, 10 parts of hovenia dulcis thunb, 5 parts of chrysanthemum and 1 part of lemon.
Embodiment two:
the bovine liver peptide anti-alcoholic formula consists of the following raw materials in parts by weight: 35 parts of beef liver, 25 parts of corn gluten meal, 15 parts of kudzuvine root, 10 parts of hovenia dulcis thunb, 5 parts of chrysanthemum and 5 parts of lemon.
Embodiment III:
the anti-alcoholic formula of the boletus peptide and the raw material preparation method thereof comprise the following raw materials in parts by weight: 35 parts of beef liver, 25 parts of corn gluten meal, 20 parts of kudzuvine root, 15 parts of hovenia dulcis thunb, 10 parts of chrysanthemum and 5 parts of lemon.
The raw material preparation method of the bovine liver peptide anti-alcoholic formula comprises the steps of weighing the bovine liver, the corn gluten meal, the kudzuvine root, the hovenia dulcis thunb, the chrysanthemum and the lemon according to the weight parts of the bovine liver, the corn gluten meal, the kudzuvine root, the hovenia dulcis thunb, the chrysanthemum and the lemon in the third embodiment, and respectively preparing the bovine liver peptide, the corn oligopeptide powder, the kudzuvine root extract, the hovenia dulcis thunb extract, the chrysanthemum extract and the lemon powder into the 6 extract extraction methods as follows:
bovine liver peptide powder: mechanically removing the outer fascia of the beef liver, cutting into 5 x 5cm blocks, soaking and cleaning, homogenizing by a colloid mill, and extracting the homogenized feed liquid by enzymolysis: adding 2 times of water, heating the raw material liquid to 55 ℃, adding animal protease (2 ten thousand U/g) with the concentration of 1% relative to the substrate, and carrying out enzymolysis for 4 hours; after enzymolysis, carrying out enzyme deactivation treatment on the raw material liquid at 90 ℃ for 30min, adding activated clay for adsorption at the same time of enzyme deactivation, wherein the adsorption time is 30min, and the adding amount is 2% of the volume of the feed liquid; the enzyme deactivation adsorption adopts a horizontal centrifuge for centrifugation, the discharge slag and the activated clay are separated, and the supernatant fluid is taken; purifying with 10kDa ultrafilter membrane; concentrating the purified feed liquid to 40% by double effect, and spray drying to obtain liver peptide powder;
corn oligopeptide powder: after the corn protein powder is dissolved by adding water, the feed liquid ratio is 1:3, heating to 45 ℃, adding alkaline protease (1 kiloU/g) and papain (2 kiloU/g) with the concentration of 0.5 percent relative to the substrate, carrying out enzymolysis for 3 hours, carrying out enzyme deactivation treatment on the raw material liquid at 90 ℃ for 30 minutes after enzymolysis, carrying out tubular separation at 14000rpm after enzyme deactivation is finished, taking supernatant, and purifying by a 1kDa ultrafiltration membrane; concentrating the purified feed liquid to 40% by double effect, and spray drying to obtain corn oligopeptide powder;
radix Puerariae extract: pulverizing radix Puerariae with 80 mesh, adding 10 times of water, leaching with 90 deg.C water for 3 hr, continuously leaching for 3 times, centrifuging, collecting supernatant, concentrating to 1/5 of the total volume with double effect concentration, and spray drying to obtain radix Puerariae extract;
semen Hoveniae extract: pulverizing semen Hoveniae into coarse powder, and leaching with 10 times of water at 70deg.C for 2-3 hr; centrifuging, collecting supernatant, concentrating by double effect concentration to 1/5 of the total volume, and spray drying to obtain semen Hoveniae extract;
flos Chrysanthemi extract: crushing chrysanthemum into coarse powder, and leaching with 10 times of water at 70 ℃ for 2-3 h; centrifuging, collecting supernatant, concentrating by double effect concentration to 1/5 of the total volume, and spray drying to obtain flos Chrysanthemi extract;
lemon powder: cleaning lemon, peeling, removing pulp and seeds, crushing, squeezing, centrifuging at 14000rpm to obtain clear lemon juice, adjusting pH to 4.5-5.5 with sodium bicarbonate, adding starch for molecular embedding, and spray drying to obtain raw lemon pulp powder.
And (3) manufacturing a finished product: the ingredients of the formula are all extracts, the ingredients are uniformly mixed by a V-shaped mixer, then the mixture is conveyed to a boiling granulator for mixing, granulating (the binder is purified water), and drying (the temperature is 135 ℃) to obtain brown small granular solid matters, and the brown small granular solid matters are filled and packaged to obtain the final product.
The specification of bagged finished products: 5 g/pack;
traits: brown granules; smell lemon flavor; lemon fruit taste with sweet taste;
preservation conditions: room temperature, sealing and light shielding;
physicochemical properties and biological characteristics: physicochemical properties and microbiological characteristics meet standard requirements;
the eating method comprises the following steps: the daily intake is less than or equal to 15g (namely 3 bags), and 600ml of warm water is used for brewing and drinking;
eating taboos: pregnant women and children under 14 years old are not suitable for taking; the patients with protein allergy cannot take the medicine.
Effect analysis
The above examples were given to 60 subjects who were drinking, aged 30-60 years, and equally divided into 2 experimental groups (formulas) and control groups (placebo), each group of 30 persons was blind, the subjects were not drinking for nearly 7 days, and the subjects were empty stomach in the test, and the same alcohol was used and the volume of alcohol was consistent (100 ml, 43 °). Blood alcohol concentration was measured 1 hour after drinking, data were recorded, and then subjects took 3 bags of product continuously, and after 1 hour, the measurement was performed again, and the change in alcohol content was analyzed in a lump. The evaluation summary results are as follows:
note that: the effect is obvious: the alcohol degree of alcohol meter test decreases by 30% -50% in unit time; the effect is effective: the alcohol degree of alcohol meter test decreases by 10% -30% in unit time; the effect is invalid: the alcohol degree of alcohol tester is reduced by less than 10% in unit time; the effective rate is as follows: effective rate (%) = (effective+significant)/group population.
From a summary table, the formula is effective in alleviating hangover, and the effective rate is 96.7%.
The present invention is not limited to the above-mentioned embodiments, and any person skilled in the art, based on the technical solution of the present invention and the inventive concept thereof, can be replaced or changed equally within the scope of the present invention.

Claims (4)

1. The bovine liver peptide anti-alcohol formula is characterized by comprising the following raw materials: ox liver, corn protein powder, kudzuvine root, hovenia dulcis thunb, chrysanthemum and lemon.
2. The bovine liver peptide anti-alcohol formula according to claim 1, which is characterized by comprising the following raw materials in parts by weight: 30-35 parts of beef liver, 20-25 parts of corn gluten meal, 15-20 parts of kudzuvine root, 10-15 parts of hovenia dulcis thunb, 5-10 parts of chrysanthemum and 1-5 parts of lemon.
3. A formulation for neutralizing the effect of alcoholic drinks of bovine liver peptide according to claim 2, wherein: the optimal proportion is composed of the following raw materials in parts by weight: 35 parts of beef liver, 25 parts of corn gluten meal, 15 parts of kudzuvine root, 10 parts of hovenia dulcis thunb, 5 parts of chrysanthemum and 5 parts of lemon.
4. A method for preparing raw materials for preparing the anti-alcoholic formulation of the boletpeptide of claim 3, which is characterized in that: the method for preparing the beef liver extract comprises the following steps of respectively weighing beef liver, corn gluten meal, kudzuvine root, hovenia dulcis thunb, chrysanthemum and lemon according to the parts by weight, and respectively preparing the beef liver peptide, corn oligopeptide powder, kudzuvine root extract, hovenia dulcis thunb extract, chrysanthemum extract and lemon powder according to the parts by weight:
bovine liver peptide powder: mechanically removing the outer fascia of the beef liver, cutting into 5 x 5cm blocks, soaking and cleaning, homogenizing by a colloid mill, and extracting the homogenized feed liquid by enzymolysis: adding 2 times of water, heating the raw material liquid to 55 ℃, adding animal protease (2 ten thousand U/g) with the concentration of 1% relative to the substrate, and carrying out enzymolysis for 4 hours; after enzymolysis, carrying out enzyme deactivation treatment on the raw material liquid at 90 ℃ for 30min, adding activated clay for adsorption at the same time of enzyme deactivation, wherein the adsorption time is 30min, and the adding amount is 2% of the volume of the feed liquid; the enzyme deactivation adsorption adopts a horizontal centrifuge for centrifugation, the discharge slag and the activated clay are separated, and the supernatant fluid is taken; purifying with 10kDa ultrafilter membrane; concentrating the purified feed liquid to 40% by double effect, and spray drying to obtain liver peptide powder;
corn oligopeptide powder: after the corn protein powder is dissolved by adding water, the feed liquid ratio is 1:3, heating to 45 ℃, adding alkaline protease (1 kiloU/g) and papain (2 kiloU/g) with the concentration of 0.5 percent relative to the substrate, carrying out enzymolysis for 3 hours, carrying out enzyme deactivation treatment on the raw material liquid at 90 ℃ for 30 minutes after enzymolysis, carrying out tubular separation at 14000rpm after enzyme deactivation is finished, taking supernatant, and purifying by a 1kDa ultrafiltration membrane; concentrating the purified feed liquid to 40% by double effect, and spray drying to obtain corn oligopeptide powder;
radix Puerariae extract: pulverizing radix Puerariae with 80 mesh, adding 10 times of water, leaching with 90 deg.C water for 3 hr, continuously leaching for 3 times, centrifuging, collecting supernatant, concentrating to 1/5 of the total volume with double effect concentration, and spray drying to obtain radix Puerariae extract;
semen Hoveniae extract: pulverizing semen Hoveniae into coarse powder, and leaching with 10 times of water at 70deg.C for 2-3 hr; centrifuging, collecting supernatant, concentrating by double effect concentration to 1/5 of the total volume, and spray drying to obtain semen Hoveniae extract;
flos Chrysanthemi extract: crushing chrysanthemum into coarse powder, and leaching with 10 times of water at 70 ℃ for 2-3 h; centrifuging, collecting supernatant, concentrating by double effect concentration to 1/5 of the total volume, and spray drying to obtain flos Chrysanthemi extract;
lemon powder: cleaning lemon, peeling, removing pulp and seeds, crushing, squeezing, centrifuging at 14000rpm to obtain clear lemon juice, adjusting pH to 4.5-5.5 with sodium bicarbonate, adding starch for molecular embedding, and spray drying to obtain raw lemon pulp powder.
CN202310897236.9A 2023-07-20 2023-07-20 Boletus peptide anti-alcoholic formula and raw material preparation method Pending CN116898098A (en)

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CN202310897236.9A CN116898098A (en) 2023-07-20 2023-07-20 Boletus peptide anti-alcoholic formula and raw material preparation method

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CN202310897236.9A CN116898098A (en) 2023-07-20 2023-07-20 Boletus peptide anti-alcoholic formula and raw material preparation method

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CN116898098A true CN116898098A (en) 2023-10-20

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