CN110269224A

CN110269224A - A kind of elder jelly and preparation method thereof

Info

Publication number: CN110269224A
Application number: CN201910653407.7A
Authority: CN
Inventors: 刘昕
Original assignee: GEOTHINK ECO TECH (SHANGHAI) Co Ltd
Current assignee: GEOTHINK ECO TECH (SHANGHAI) Co Ltd
Priority date: 2019-07-19
Filing date: 2019-07-19
Publication date: 2019-09-24

Abstract

The invention discloses a kind of preparation method of elder jelly, include the following steps: to prepare synthetism rasp berry inspissated juice, prepare flores sambuci inspissated juice, change mass, ingredient is filling, sterilizing and drying.Jelly flavor grace of the invention is pure and fresh, and delicate mouthfeel is convenient, has preventing cold, improves the immunity of the human body, product wide market.

Description

A kind of elder jelly and preparation method thereof

Technical field

The present invention relates to the preparation methods of jelly more particularly to a kind of elder jelly and preparation method thereof.

Background technique

Sambucus (Sambucus) is a category in Caprifoliaceae (Caprifoliaceae), and the whole world has more than 40, It is distributed in the temperate zone and subtropical zone of two hemispheres, China is there are about 15 kinds, and resourceful, yield is very high, it is distributed southwards from northeast, To the north of Nanling, west to SOUTH OF GANSU and Sichuan, Yunnan (southeast).

Elder is gained the name because it has effects that synthetism, is a kind of medicinal plant of preciousness, can complete stool be used as medicine.It is main to use To treat the illnesss such as fracture, traumatic injury, rheumatic arthritis, gout, Kaschin-Beck disease, oedema.It is recorded in Compendium of Material Medica Release entitled symplectic bone wood, the wooden capsule.Gu has the laudatory title of " catholicon ", and ancient Egyptian thinks that it gets rid of evils and flees from evil, and can prevent The serious illness is burdened with Zaphie；Gypsy just had before centuries is used to treat chill, influenza and branch gas for elder The history of pipe discomfort.Elder is widely used in Europe, there is the good reputation of " the earth jewel ", " omnipotent medical box ", hands in season For when facilitate adjust constitution.

Elder berry (Elderberries), bitter, pungent, gas is flat, can promote sweating, anti-schneiderian membrance inflammation, diuresis, helps body Body resists cold, flu.Flores sambuci (Sambucus nigra), property and flavor of peppery and warm enter lung, bladder meridian, are sudorific, " traditional Chinese medicines Summary " in have relevant record.It is then recorded in " pharmacology of traditional Chinese medicines " " for medicinal tea, to be used for sweating, and have the benefits of diuresis." connect Bone wood is annual five, the edelweiss of slightly milk yellow is bloomed between June, and pure and fresh pleasant fragrant and sweet taste and muscat are very close, Elder jasmine tea can be made.Tea is drunk while hot, can be promoted blood circulation, sweating, various poison in body are also beneficial to The discharge of element.It is drunk in common cold initial stage, helps to mitigate the symptoms such as sneezing, rhinorrhea, aversion to cold.

Elder berry polyphenols containing a large amount and plant element, such as flavones and flavonoid glycoside (Quercetin, rutin sophorin), cyanine Element, hemagglutinin SNA- III, organic acid, vitamin.Johansen isolates 4 kinds of cyanine from Canadian erberry Glycosides.Nakatani etc. isolates 2 kinds of new acylation cyanine glycosides compounds from the fruit.The researchs such as Du Fengguo find elder Vitamin C rich in the certain kind of berries has very important function to human body.Contain 17 kinds of amino acid in fruit, 7 kinds are human body Essential amino acid accounts for 30.62%.Synthetism rasp berry, which contain, enriches beneficial mineral element, and potassium, calcium, phosphorus content are very high, and are free of The ingredient of lead.

Flores sambuci contains essential oil, triterpene, flavonoids, lymphatic temperament, tannin, colloid, the main components such as carbohydrate.Lamaison Deng isorhamnetin -3- glucosides isolated from black flores sambuci and 3- rutin glycosides.Kislichenko etc. is from European elder 16 kinds of amino acid are separated in colored extract, wherein there are 9 kinds is essential amino acid.

Studies have shown that the abundant anthocyanidin and bioflavonoid that contain in erberry, there is antiviral and raising to exempt from The effect of epidemic disease power.The researchs such as Barak find black elderberries reality extract in vitro experiment, can inhibit 10 kinds of influenza viruses. By double blind experiment, the patient for taking extract Antibody of Influenza in convalescent serum is apparently higher than control group, and β-IL-1, α-TNF and IL-6 content can be made significantly to increase.The researchs such as Zakay-Rones think that black elderberries extract object in fact Red cell agglutination can be reduced outside, and inhibits the influenza virus A of most of cultures, the duplication of B.Researcher once gives flu victims The extract is taken orally, cures 90% patient completely in 2-3d, the duration of flu symptom is obviously shortened. The researchs such as Caceres find that Mexico's elderberry extract has the active effect of anti-dermatophyte.The researchs such as Yesilada hair Existing danewort extract concentrations have stronger helicobacter pylori resistant to act in 1.95-250g/L.Elder is lived immune Property in terms of effect: Anon etc. the study found that golden elder can by adjust human cytokine adjust immunity, Therefore the fruit juice and wine product made using Sambucus plant as raw material is the drink beneficial to human health.Sambucus plant fruit Anthocyanidin contained in reality can enhance the immune function of human body by promoting the production of cytokine.

Jelly is one of favorite food of modern, however the nutritive value of traditional jelly is not but high.As consumer is raw The flat raising of running water, the reinforcement of health care consciousness, requirement of the consumer to snack food are higher and higher.Not only need delicious, safety, Health, or even also to have the function of health care dietotherapy.Jelly on the market is not built up resistance as snack food, prevention The functional jelly of flu.

For the children for being in growth and development stage, sweet food is eaten for a long time, it is useless that organism metabolism generates a large amount of metabolism Object pyruvic acid, lactic acid etc. lead to children Yi Chengwei acidic constitution and are deficient in vitamin, to influence development and the function of children's brain The maintenance of energy.For the crowd people of hyperglycemia, edible rear blood sugar concentration undoubtedly will increase.The sweetener that jelly is selected is in addition to white The synthetic sweeteners such as food additives saccharin sodium, honey element, Aspartame are also added in granulated sugar, glucose, part.These additives Normal additive amount, without harm, excessively will lead to internal food additives and excessively accumulate to human body itself if it is edible, Equally body can also be adversely affected.

The food that elder is developed as raw material at present, there is elder Yoghourt, elder in ground zero, market at home The minority product such as syrup is partially elder flavour product made of addition elder essence.There has been no concentrations currently on the market The Related product of synthetism rasp berry juice and concentration flores sambuci extracting solution, there has been no maceraring enzyme combination film concentrations, and use super-pressure Sterilization technology prepares the open report of synthetism rasp berry inspissated juice, and also there has been no ultra high pressure extraction combination film filterings, and using sterile Cold filling technology prepares the open report of flores sambuci inspissated juice.

Synthetism rasp berry, flores sambuci composite yeast beta glucan are added, further enhance health value mutually；Using Healthy and beneficial low calorie sweetener substitutes sucrose；The natural gelling agent of compatibility.Have using the above product as raw material to prepare The elder jelly of health-care efficacy, rare finding, substantially belongs to blank at home.Product also has small in size, carrying, edible letter Single, quick feature, can be increasingly by the welcome of consumer.Elder jelly is that new way is opened up in the development and utilization of elder Diameter has good market prospects.

Maceraring enzyme (Macerating enzyme) is also known as macerating enzyme, it is a kind of complex enzyme, is mainly used for garden stuff processing, Broken fruit is acted on, crushing juice rate is improved；Simultaneously can promote to filter, reduce production energy consumption, keep fruits and vegetables original local flavor and Improve its biological stability etc..Since the enzyme range of hydrolysis of this kind of single variety of pectase is relatively narrow, enzymolysis efficiency is not so good as by several The maceraring enzyme of the kind compound composition of ingredient is high.The report of the method for fruit tree fruit juice is connect with maceraring enzyme enzymatic hydrolysis synthetism rasp berry juice preparation at present Road has not been reported.

Super-pressure (ultra-high pressure, UHP) or high static pressure (high hydrostatic pressure, HHP) processing technology refers in closed ultrahigh pressure vessel, is that medium is pressurized under conditions of room temperature or heating with water or oil 100-1000MPa handles flexible packaging food, new to reach one kind of the purpose of sterilization, blunt enzyme, extraction and processed food Emerging foods processing technique.Ultra high pressure extraction technology due to extraction efficiency is high, extraction time is short, impurity component dissolution less, The features such as extract component activity is high starts to be applied in the extraction of active components of plants.

Oligofructose is a kind of special water-soluble dietary fiber, has not only maintained the pure sweetening characteristics of sucrose, but also compare sugarcane Sugared sweet taste is salubrious.Oligofructose is that have to adjust intestinal flora, is proliferated Bifidobacterium, and relief of constipation promotes the absorption of calcium, adjusts Blood lipid, immunological regulation, the novel sweetener of the healthcare functions such as anti-caries tooth.Oligofructose is not easy to be absorbed by the body, and calorie value is very low, It not will lead to obesity, it is very popular.

Antierythrite, calorific value is low, about 1/10th of sucrose calorific value, and calorie value is minimum in all sugar alcohols, no It will lead to undesirable stomach reaction.Antierythrite has substantially no effect on blood glucose and insulin level in blood, is suitable for sugar Urinate patient.Without saprodontia.

Isomaltoketose, also known as isomery sucrose, using white granulated sugar as raw material, through sucrose isomerase enzymatic conversion, then concentrated, knot It is the new sugar source of low sweet tea with alimentary health-care function made of brilliant, separation.It can continue, slowly, balancedly provide energy, mentions Highly anti-fatigue ability, low glycemic index and low pancreas islet index promote fat oxidation, masking peculiar smell, balance taste and flavor, protection Tooth improves cerebral function, attention is kept to concentrate.

It is raw material through prepared by chlorination that Sucralose, also known as Sucralose, which are using sucrose,.It is with noenergy, sweet tea The features such as degree is high, and sweet taste is pure, highly safe is one of current classic functional sweetener.

Yeast beta-dextran is the polysaccharide that D-Glucose monomer is formed by glucosides key connection, and general chemical structure is β -1, 3 glucans and β -1,6 glucan.It is with many lifes such as strengthen immunity, anti-anti-inflammatory, antibacterial, anti-infective, antiviral, anticancers Manage function.

Citric acid is mainly used for assigning product tart flavour, adjusts product special flavour, and reduces pH value, inhibits bacterial growth.Lemon Sour sodium is a kind of acidity regulator.

Therefore, those skilled in the art is dedicated to developing a kind of preparation method of elder jelly.

Summary of the invention

In view of the above drawbacks of the prior art, the technical problem to be solved by the present invention is to how prepare elder berry Freeze.

To achieve the above object, the present invention provides a kind of preparation methods of elder jelly, which is characterized in that including such as Lower step:

(1) it prepares synthetism rasp berry inspissated juice: being crushed cleaned elder berry to obtain slurries with crusher, by congee Change and digested in enzyme addition slurries, filter, obtain the first extracting solution and slag, RO water is added into slag and obtains slag liquid, slag and RO The ratio of water is 1:3~4, extracts 0.5~1h under conditions of 40~55 DEG C to slag liquid, the second extracting solution is obtained by filtration, and is merged First extracting solution and the second extracting solution obtain synthetism rasp berry juice, are centrifuged to obtain the first supernatant to synthetism rasp berry juice, will be on first Clear liquid by molecular cut off be 100KDa ultrafiltration membrane obtain permeate, will transmit through liquid be concentrated into soluble solid be 25~ 30%, then sterilize under 400-600MPa pressure to obtain synthetism rasp berry inspissated juice；

(2) it prepares flores sambuci inspissated juice: crushing flores sambuci, smashed flores sambuci is extracted to obtain third Extracting solution, is centrifuged third extracting solution to obtain the second supernatant, filtered solution is obtained to the second supernatant liquid filtering, to filter It crosses liquid to carry out being concentrated into soluble solid being 15~20%, and then sterilizes and obtain flores sambuci inspissated juice；

(3) change mass: by carragheen, gellan gum, tamarind gum, oligofructose, antierythrite, isomaltoketose, three Chlorine sucrose and yeast beta-dextran are uniformly mixed so as to obtain glycocoll mixture, after room temperature RO water is added into glycocoll mixture, through colloid mill It is put into after milled and boils material pot, boiled material to abundant dissolution and obtain feed liquid, feed liquid is then cooled to 85~95 DEG C；

(4) ingredient: calcium lactate is dissolved with 80~90 DEG C of RO water, dissolved calcium lactate is added to the feed liquid of step (3) In, after at least 1min, the synthetism rasp berry inspissated juice of step (1) and the flores sambuci inspissated juice of step (2) is added, every at least After 1min, the additive of 80~90 DEG C of 5.9~6.8 parts of RO water dissolutions is added, obtains semi-finished product solution, detects semi-finished product solution Color, flavor, mouthfeel, pH, soluble solid and feed liquid forming degree, when semi-finished product solution soluble solid be 18.5 ± 0.2%, pH is 3.7 ± 0.1, flavor is synthetism rasp berry juice flavor, color is aubergine, and semi-finished product solution is qualification；

(5) filling: filtering and encapsulating while hot being carried out to qualified semi-finished product solution, are fills up to jelly package with filling machine Interior, at 73-75 DEG C, each batch of material control is filled in 40min for filling temperature control, sterilizes immediately after the completion of filling；

(6) sterilize: sterilising temp is 80~85 DEG C, 15~20min of sterilization time, is put into immediately after sterilizing cold in cold water But, the water temperature of cooling bay is controlled at 20 DEG C hereinafter, be no less than 15min cooling time, is cooled to room temperature；

(7) dry: drying temperature is not higher than 40 DEG C, and elder jelly is made.

Further, the temperature of the enzymatic hydrolysis in step (1) is 45~55 DEG C, and enzymolysis time is 1~2h.

Further, the additive amount of the maceraring enzyme in step (1) is the 0.05~0.38% of fruit weight.Preferably, maceraring enzyme Dosage be fruit weight 0.05-0.1%.

Further, the composition ratio of maceraring enzyme are as follows: pectase 6000-8000 μ/g, cellulase 400-800 μ/g, wood Dextranase 2000-3000 μ/g.

Further, the process of the extraction in step (2) is that RO water is added into smashed flores sambuci, smashed The ratio of flores sambuci and RO water is 1:12~15, and citric acid is added, and the additive amount of citric acid is the 2% of RO water, 250~ Under conditions of 300MPa, 5~15min of pressure maintaining.

Further, the temperature of the sterilizing in step (2) is 130-135 DEG C, sterilization time 5-15s.

Further, the additive in step (4), including citric acid and sodium citrate.

The present invention also provides a kind of elder jelly, which is characterized in that score meter by weight, including the concentration of synthetism rasp berry 13~25 parts of juice, 4.5~6.5 parts of flores sambuci inspissated juice, 0.25~0.4 part of carragheen, 0.08~0.12 part of gellan gum, Luo Wang 0.11~0.14 part of sub- glue, 3.2~7.0 parts of oligofructose, 3.0~7.0 parts of antierythrite, 2.9~4.5 parts of isomaltoketose, 0.02~0.025 part of Sucralose, 0.15~0.25 part of yeast beta-dextran, 0.25~0.35 part of citric acid, sodium citrate 0.20~0.25 part, 0.25~0.32 part of L-calcium lactate.

Further, specific retention is 79.4~83.9%, and procyanidins content is 1.35~1.68mg/g.

Technical effect

(1) present invention handles synthetism rasp berry using maceraring enzyme, and the more single enzyme of efficiency is high, and crushing juice rate is high.Using being limited Dosage and ratio maceraring enzyme, effectively promote the ingredients such as pectin, cellulose in elder enzyme to degrade in a short time, promote to connect Bone rasp berry fruit juice liquid is rapidly discharged from cell.

(2) method for preparing synthetism rasp berry inspissated juice does not use only maceraring enzyme processing synthetism rasp berry, to improve enzymatic hydrolysis Efficiency shortens enzymolysis time, improves fruit juice yield；It is combined using by film hyperfiltration technique and reverse osmosis technology, fruit juice is carried out It filters and is concentrated, effectively solve nutriment and flavor component in vacuum concentration fruit juice and be destroyed, retain fruit juice to the full extent Mouthfeel, sterilized using low-temperature ultrahigh-pressure, sterilization effect in addition to be able to satisfy it is edible, save etc. quality requirements while, can also protect Nutrition, flavor components in full fruit juice.

(3) method for preparing flores sambuci inspissated juice is extracted using superelevation platen press, and extraction time is short, and low energy consumption, the present invention The technique membrane technology of flores sambuci inspissated juice is concentrated, and UHT combination cryogenic sterile cold filling technique, production process is efficient, maximum journey The flavor of this fragrant plant of flores sambuci is remained on degree.

(4) be concentrated synthetism rasp berry juice, flores sambuci extracting solution and sweetener oligofructose, antierythrite, isomaltoketose, Sucralose and acidity regulator citric acid, sodium citrate form optimal sugar-acid ratio under the parts by weight, keep jelly sour Sweet tea is palatable, is full of fruit juice sense.Isomaltoketose can cover the peculiar smell that elderberry juice and yeast beta-dextran slightly have, balance port Sense and flavor.

(5) gelling agent selects carragheen, gellan gum and tamarind.Carragheen selects lota type carragheen.Gellan gum selects low Acyl gellan gum.Calcium salt is reacted with gellan gum, carragheen, can form gel structure.Gellan gum can form transparent, hard brittle Brittleness gel, have pure and fresh tasty and refreshing mouthfeel.Proper amount of carragheen can form soft with calcium binding and have bullet The gel of property.In addition, calcium lactate, since dissolubility is preferable, uniform gelling easy to form is preferably used as gelling agent.Add Add the calcium lactate of doses, can not only stable prod gel rubber system, also improve nutritive value.When gellan gum and carragheen are mixed A variety of textures will be automatically generated when conjunction, the excessive then jelly of the proportion of gellan gum and carragheen can be partially hard, and too small then jelly can mistake It is soft.The tamarind gum of gel be it is a kind of extracted from tamarind have excellent salt tolerant, acidproof and thermal stability polysaccharide Substance.Tamarind gum plays synergy, while reducing gellan gum and carragheen additive amount, also enhances jelly Water-retaining property.Gellan gum, carragheen and tamarind gum pass through reasonable compatibility under the conditions of suitable soluble solid, elder Jelly quality is fine and smooth, retentiveness is good, and elder Flavor release is good, there is the mouthfeel of just melt in the mouth, can feel at ease to eat.

(6) present invention combines concentration technique using film, nutriment in synthetism rasp berry juice caused by avoiding because of evaporator concentration With the destruction of flavor component, retain the mouthfeel of fruit juice to the full extent.

(7) present invention substitutes traditional heat sterilization means with super-pressure, and it is original to maintain synthetism rasp berry juice for normal-temperature operation Mouthfeel, utmostly avoid nutritional ingredient caused by high temperature sterilization destroy and flavor components loss.

(8) present invention is small to the active skull cap components destruction in flores sambuci using ultra high pressure extraction, extraction time is short, energy Consume the features such as low.

(9) present invention reasonable compatibility gellan gum, carragheen and tamarind gum, jelly matter under the conditions of soluble solid Ground is fine and smooth, retentiveness is good, and elder Flavor release is good, there is the mouthfeel of just melt in the mouth, can feel at ease to eat.

(10) preparation method working specification of the present invention, quality monitoring are stringent, it is ensured that the safety of product and can By property.

(11) under 400-600MPa pressure, bacterium, saccharomycete, mould can be killed, avoids general high temperature sterilization band The disadvantageous changes come, and the nutritional ingredient in the intact retaining food product of energy, food mouthfeel is good, and color is natural, highly-safe, guarantees the quality Phase is long, this is the advantages of conventional high-temperature heat sterilization method is not had.

Elder jelly of the invention has the advantage that

(a) elder jelly provided by the invention selects natural gelling agent to be matched with synthetism rasp berry and flower for raw material 5, it adds a variety of sweeteners low in calories and substitutes sucrose, add yeast beta-dextran, no added essence, pigment and preservative.It connects Bone wood jelly is in aubergine, and present-color material is natural pigment, natural, graceful.Its sweet and sour taste, the fruity with synthetism rasp berry. Elder jelly provided by the invention, smooth to mix delicious fruit juice, the refrigerant sense of pleasant stroke and mistake, tool in throat There is good Flavor release, there is the mouthfeel of just melt in the mouth.Jelly retentiveness is good, delicate mouthfeel, can feel at ease to eat.

(b) since elderberry juice slightly has astringent taste, be not suitable for being made into 100% pure juice beverage.And it provided by the invention connects Bone wood jelly, it is small in size, simply eaten, quick, light and handy suction nozzle packaging is carried, can be tasted at any time.

(c) elder jelly provided by the invention has balanced human's immunity, has good prevention effect for flu, Help to reduce the incidence caught a cold, mitigate influenza bring discomfort degree, shortens the duration.Day can also be used as Normal oxidation resistant supplement.

(d) elder jelly provided by the invention has health using oligofructose, antierythrite, isomaltoketose etc. The low calorie sweetener of benefit substitutes sucrose, glucose, reduces the intake of heat.

The technical effect of design of the invention, specific structure and generation is described further below, to be fully understood from The purpose of the present invention, feature and effect.

Specific embodiment

Multiple preferred embodiments of the invention introduced below keep its technology contents more clear and are easy to understand.The present invention It can be emerged from by many various forms of embodiments, protection scope of the present invention is not limited only to the reality mentioned in text Apply example.

It is described herein and " RO water " be commercially available pure water；" UHT " refers to uht sterilisation method, is by very short time Inside it is warmed as the method for affairs sterilizing；" % " according to the present invention refers to weight percent.

Embodiment 1:

By weight percentage include following components: flores sambuci extracting solution 5.9 is concentrated in 13.6 parts of synthetism rasp berry juice of concentration Part, 0.32 part of carragheen, 0.10 part of gellan gum, 0.11 part of tamarind gum, 6.7 parts of oligofructose, 3.0 parts of antierythrite, different wheat 2.9 parts of bud ketose, 0.02 part of Sucralose, 0.18 part of yeast beta-dextran, 0.35 part of citric acid, 0.25 part of sodium citrate, L- 0.28 part of calcium lactate, remaining is RO water, and total amount is 100 parts.

(1) it prepares synthetism rasp berry inspissated juice: synthetism rasp berry being frozen after fruit is thawed and are washed with water completely, pest and disease damage, corruption are sorted out Rotten fruit.Juicing: cleaned elder berry is crushed with crusher, breaks into pulpous state.Enzymatic hydrolysis: maceraring enzyme is added and is starched It is digested in liquid, 1.5h is digested under conditions of 50 DEG C, the additive amount of maceraring enzyme is 0.3%.Filtering, obtains the first extracting solution And slag, RO water is added into the slag that extraction obtains, solid-liquid ratio=1:4 extracts 0.5h under conditions of 50 DEG C, is obtained by filtration second Extracting solution.Merge the first extracting solution and the second extracting solution, obtains synthetism rasp berry juice.Centrifugation: the centrifuge separation of horizontal spiral seperator, Vibrating screen takes out supernatant.Filtering: supernatant is made to obtain permeate by the ultrafiltration membrane of molecular cut off 100KDa.Concentration: Permeate is concentrated into soluble solid 25% through reverse osmosis membrane again, and every 1mL concentrate is equivalent to original fruit juice 2.7g.Sterilization: Under the ultra-high pressure condition of 550MPa, synthetism rasp berry juice must be concentrated in pressure maintaining sterilizing 8min.

(2) prepare flores sambuci inspissated juice: the selected removal of impurities of flores sambuci crushed 60 mesh.It extracts: ultra high pressure extraction tank It extracts, RO water, solid-liquid ratio 1:15 is added into elder dried flower.Citric acid is added, additive amount is the 2% of RO water.In 300MPa Ultra-high pressure condition under, pressure maintaining 8min.Centrifugation: extracting solution is taken out after release, is existed after coarse filtration through being pumped into disc centrifuge It is centrifuged under conditions of 4800r/min, isolates supernatant.Filtering: with the ceramic membrane filter of aperture 100nm.Concentration: reverse osmosis concentrated It is reduced to soluble solid 15%, every 1mL concentrate is equivalent to former flores sambuci 10g.Sterilizing: cold-aseptic filling, UHT sterilizing temperature 131 DEG C, 15s of degree, 30 DEG C of the filling temperature < of liquid, obtains concentration flores sambuci extracting solution out.Using the technique of ceramic membrane filter Do not add foreign substance, such as the gelatin of clarification, diatomite auxiliary agent realize the clarification of flores sambuci extracting solution.Above-mentioned auxiliary agent Substance adds the content that can reduce Flavonoid substances in flores sambuci, in some instances it may even be possible to have an impact to flavor.

(3) change mass: by carragheen, gellan gum, tamarind gum, oligofructose, antierythrite, isomaltoketose, three After chlorine sucrose and yeast beta-dextran mix, 57 parts of room temperature RO water is added, enters to boil material pot after colloid mill milled.Open steam Material is boiled, boils material to 95 DEG C of sufficiently dissolutions.Feed liquid is cooled to 90 DEG C.

(4) with 3 parts of the RO water dissolution calcium lactate for being pre-heated to 85 DEG C, dissolved calcium lactate ingredient: is added upper one It walks in feed liquid.After at least 1min, addition is preheated to 70 DEG C of concentration synthetism rasp berry juice and concentration flores sambuci extracting solution.Every After at least 1min, addition is pre-heated to the additives of 85 DEG C of 6.8 parts of RO water dissolutions, and wherein additive includes citric acid, lemon Sour sodium.The semi-finished product of 10-15g are taken to detect color, flavor, mouthfeel, pH, soluble solid and the feed liquid forming degree of solution, it can Dissolubility solid content 18.5 ± 0.2%, pH3.7 ± 0.1, flavor are synthetism rasp berry juice flavor, color is aubergine, and detection is qualified Afterwards, after the filtering of 80 mesh, through 100 mesh filters to heat insulation tank, the temperature of fireless cooker will be controlled at 76-78 DEG C pumping material.

(5) filling: to start to filter simultaneously encapsulating, 100 mesh of filling machine material cylinder filter bag after qualified while hot.It is fills up to filling machine In jelly package, filling temperature control is at 73-75 DEG C, and filling specification 35g/ bags.Each batch of material control is filled in 40min, It is sterilized immediately after the completion of filling.

(6) sterilize: sterilization water management sterilizes 18min at 83 DEG C.Cooling in cold water is put into after sterilizing immediately, in order to avoid when long Between high temperature colloid.The water temperature of cooling bay is controlled at 20 DEG C hereinafter, be no less than 15min cooling time, is cooled to room temperature, is made Finished product.

(7) dry: drying temperature should not be greater than 40 DEG C in drying course.

Embodiment 2:

By weight percentage include following components: flores sambuci extracting solution 6.1 is concentrated in 15.2 parts of synthetism rasp berry juice of concentration Part, 0.27 part of carragheen, 0.08 part of gellan gum, 0.14 part of tamarind gum, 4.2 parts of oligofructose, 4.5 parts of antierythrite, different wheat 3.9 parts of bud ketose, 0.02 part of Sucralose, 0.22 part of yeast beta-dextran, 0.30 part of citric acid, 0.20 part of sodium citrate, L- 0.25 part of calcium lactate, remaining is RO water, and total amount is 100 parts.

(1) it prepares synthetism rasp berry inspissated juice: synthetism rasp berry being frozen after fruit is thawed and are washed with water completely, pest and disease damage, corruption are sorted out Rotten fruit.Juicing: cleaned elder berry is crushed with crusher, breaks into pulpous state.Enzymatic hydrolysis: maceraring enzyme is added and is starched It is digested in liquid, 2h is digested under conditions of 50 DEG C, the additive amount of maceraring enzyme is 0.2%.Filtering, obtain the first extracting solution and Slag, RO water is added into the slag that extraction obtains, and solid-liquid ratio=1:3 extracts 0.5h under conditions of 50 DEG C, is obtained by filtration second and mentions Take liquid.Merge the first extracting solution and the second extracting solution, obtains synthetism rasp berry juice.Centrifugation: the centrifuge separation of horizontal spiral seperator, vibration Dynamic sieve, takes out supernatant.Filtering: supernatant is made to obtain permeate by the ultrafiltration membrane of molecular cut off 100KDa.Concentration: thoroughly It crosses liquid and is concentrated into soluble solid 28% through reverse osmosis membrane again, every 1mL concentrate is equivalent to original fruit juice 3g.Sterilization: Under the ultra-high pressure condition of 500MPa, synthetism rasp berry juice must be concentrated in pressure maintaining sterilizing 10min.

(2) prepare flores sambuci inspissated juice: the selected removal of impurities of flores sambuci crushed 60 mesh.It extracts: ultra high pressure extraction tank It extracts, RO water, solid-liquid ratio 1:12 is added into elder dried flower.Citric acid is added, additive amount is the 3% of RO water.In 300MPa Ultra-high pressure condition under, pressure maintaining 10min.Centrifugation: extracting solution is taken out after release, is existed after coarse filtration through being pumped into disc centrifuge It is centrifuged under conditions of 4800r/min, isolates supernatant.Filtering: with the ceramic membrane filter of aperture 100nm.Concentration: reverse osmosis concentrated It is reduced to soluble solid 20%, every 1mL concentrate is equivalent to former flores sambuci 13.3g.Sterilizing: cold-aseptic filling, UHT sterilizing 132 DEG C of temperature, 15s, 30 DEG C of the filling temperature < of liquid, obtains concentration flores sambuci extracting solution out.

(3) change mass: by carragheen, gellan gum, tamarind gum, oligofructose, antierythrite, isomaltoketose, three After chlorine sucrose and yeast beta-dextran mix, 55 parts of room temperature RO water is added, enters to boil material pot after colloid mill milled.Open steam Material is boiled, boils material to 95 DEG C of sufficiently dissolutions.Feed liquid is cooled to 90 DEG C.

(4) with 3 parts of the RO water dissolution calcium lactate for being pre-heated to 85 DEG C, dissolved calcium lactate ingredient: is added upper one It walks in feed liquid.After at least 1min, addition is preheated to 70 DEG C of concentration synthetism rasp berry juice and concentration flores sambuci extracting solution.Every After at least 1min, addition is pre-heated to the additives of 85 DEG C of 6.3 parts of RO water dissolutions, and wherein additive includes citric acid, lemon Sour sodium.The semi-finished product of 10-15g are taken to detect color, flavor, mouthfeel, pH, soluble solid and the feed liquid forming degree of solution, it can Dissolubility solid content 19.6 ± 0.2%, pH3.7 ± 0.1, flavor are synthetism rasp berry juice flavor, color is aubergine, and detection is qualified Afterwards, after the filtering of 80 mesh, through 100 mesh filters to heat insulation tank, the temperature of fireless cooker will be controlled at 76-78 DEG C pumping material.

Embodiment 3:

By weight percentage include following components: flores sambuci extracting solution 4.5 is concentrated in 24.5 parts of synthetism rasp berry juice of concentration Part, 0.35 part of carragheen, 0.12 part of gellan gum, 0.12 part of tamarind gum, 3.2 parts of oligofructose, 7 parts of antierythrite, different malt 4.5 parts of ketose, 0.025 part of Sucralose, 0.15 part of yeast beta-dextran, 0.25 part of citric acid, 0.25 part of sodium citrate, L- cream 0.32 part of sour calcium, remaining is RO water, total amount 100.

(1) it prepares synthetism rasp berry inspissated juice: synthetism rasp berry being frozen after fruit is thawed and are washed with water completely, pest and disease damage, corruption are sorted out Rotten fruit.Juicing: cleaned elder berry is crushed with crusher, breaks into pulpous state.Enzymatic hydrolysis: maceraring enzyme is added and is starched It is digested in liquid, 1.5h is digested under conditions of 50 DEG C, the additive amount of maceraring enzyme is 0.32%.Filtering, obtains the first extraction RO water is added into the slag that extraction obtains for liquid and slag, and solid-liquid ratio=1:3.5 extracts 1h under conditions of 50 DEG C, is obtained by filtration the Two extracting solutions.Merge the first extracting solution and the second extracting solution, obtains synthetism rasp berry juice.Centrifugation: horizontal spiral seperator centrifugation point From vibrating screen takes out supernatant.Filtering: supernatant is made to obtain permeate by the ultrafiltration membrane of molecular cut off 100KDa.It is dense Contracting: permeate is concentrated into soluble solid 30% through reverse osmosis membrane again, and every 1mL concentrate is equivalent to original fruit juice 3.2g.Sterilization: Under the ultra-high pressure condition of 500MPa, synthetism rasp berry juice must be concentrated in pressure maintaining sterilizing 12min.

(2) prepare flores sambuci inspissated juice: the selected removal of impurities of flores sambuci crushed 60 mesh.It extracts: ultra high pressure extraction tank It extracts, RO water, solid-liquid ratio 1:15 is added into elder dried flower.Citric acid is added, additive amount is the 2.5% of RO water.? Under the ultra-high pressure condition of 250MPa, pressure maintaining 15min.Centrifugation: extracting solution is taken out after release, be pumped into after coarse filtration disc-type from Scheming is centrifuged under conditions of 4800r/min, isolates supernatant.Filtering: with the ceramic membrane filter of aperture 50nm.Concentration: anti- Osmosis concentration to soluble solid 15%, every 1mL concentrate is equivalent to former flores sambuci 10g.Sterilizing: cold-aseptic filling, UHT 132 DEG C of sterilising temp, 12s, 30 DEG C of the filling temperature < of liquid, obtains concentration flores sambuci extracting solution out.

(3) change mass: by carragheen, gellan gum, tamarind gum, oligofructose, antierythrite, isomaltoketose, three After chlorine sucrose and yeast beta-dextran mix, 45 parts of room temperature RO water is added, enters to boil material pot after colloid mill milled.Open steam Material is boiled, boils material to 95 DEG C of sufficiently dissolutions.Feed liquid is cooled to 90 DEG C.

(4) ingredient: with 3.5 parts of the RO water dissolution calcium lactate for being pre-heated to 85 DEG C, dissolved calcium lactate is added In one step feed liquid.After at least 1min, addition is preheated to 70 DEG C of concentration synthetism rasp berry juice and concentration flores sambuci extracting solution. After at least 1min, addition is pre-heated to the additives of 85 DEG C of 5.9 parts of RO water dissolutions, and wherein additive includes citric acid, lemon Lemon acid sodium.The semi-finished product of 10-15g are taken to detect color, flavor, mouthfeel, pH, soluble solid and the feed liquid forming degree of solution, Soluble solid 24.4 ± 0.2%, pH3.7 ± 0.1, flavor are synthetism rasp berry juice flavor, color is aubergine, and detection is qualified Afterwards, after the filtering of 80 mesh, through 100 mesh filters to heat insulation tank, the temperature of fireless cooker will be controlled at 76-78 DEG C pumping material.

(5) filling: to start to filter simultaneously encapsulating, 100 mesh of filling machine material cylinder filter bag after qualified while hot.It is fills up to filling machine In jelly package, filling temperature control is at 73-75 DEG C, and filling specification 50g/ bags.Each batch of material control is filled in 40min, It is sterilized immediately after the completion of filling.

(6) sterilize: sterilization water management sterilizes 15min at 85 DEG C.Cooling in cold water is put into after sterilizing immediately, in order to avoid when long Between high temperature colloid.The water temperature of cooling bay is controlled at 20 DEG C hereinafter, be no less than 15min cooling time, is cooled to room temperature, is made Finished product.

Embodiment 4:

According to table 1 to the spy of color, flavor, mouthfeel and the tissue morphology of embodiment 1-3 elder jelly totally four aspects Sign compares evaluation, and evaluation result is specifically as shown in table 2.Items are respectively 25 points, and full marks are 100 points.Choose 30 food Professional carries out sensory testing according to the standard, is averaged as appraisal result, is determined test result.

1 sensory quality assessment standard of table

2 elder jelly feature grade form of table

The measurement of retentiveness

A certain amount of jelly is taken to set in centrifugal filter (volume 5mL, 0.45 μm of filter plate aperture), under the conditions of 2000rpm It is centrifuged 30min, is weighed immediately after centrifugation, the water holding of the ratio of the quality before quality and centrifugation after being centrifuged using jelly as jelly Property.Testing result is as follows: the specific retention 79.4% of embodiment 1, the specific retention 83.9% of embodiment 2, the specific retention of embodiment 3 82.6%.

Embodiment 5:

To pollutant limitation (lead), microorganism limitation in 3 gained elder jelly of embodiment 1, embodiment 2 and embodiment (total plate count, coliform, mould) is detected, the results showed that detection project pollutant limitation, microorganism limitation meet Relevant regulations in GB19299-2015.

The procyanidine ingredient of embodiment is determined, using standard GB/T 22244-2008, testing result is such as Under: the 1.35mg/g of content containing procyanidins in embodiment 1, the 1.68mg/g of content containing procyanidins in embodiment 2, in embodiment 3 The 1.42mg/g of content containing procyanidins.

Embodiment 6:

The test of chmice acute Oral toxicity: using maximum tolerated dose method, selects female, male SPF grades of ICR mouse each 10. Concentration synthetism rasp berry juice is taken, is concentrated after flores sambuci extracting solution and yeast beta-dextran are amplified by 2 equal proportion of embodiment and adds Into tested material, concentration is added up to 40g/100g (can stomach-filling maximum concentration), remaining supplementary material is added by the concentration of embodiment 2, Intragastric administration on mice is made by the production method and gives jelly tested material.Fasting 16h gives tested material, stomach-filling in interior stomach-filling for 24 hours Amount is 0.2g/10g BW, and being converted into dosage is 20g/kg BW, distilled water of the control group to equal volume.Give tested material 14d Ordinary circumstance, poisoning manifestations and the death condition of interior observation mouse.Gross anatomy is done to dead animal, and is weighed in 14d Weight calculates weight gain.

Experimental result: after mouse empty stomach stomach-filling, being observed continuously 14d, and the activity of each group mouse is normal during test, and hair color is just Often, have no that obvious poisoning symptom, no an example are dead.Animal is put to death after 14d, Organs of Mice no abnormality seen is visually observed, because giving Medicine body product, concentration have reached to greatest extent, therefore maximum tolerated dose (MTD) > 15g/kg.It, should according to acute toxicity dose fractionation Sample belongs to nontoxic grade.

Embodiment 7:

Mouse marrow cell micro nuclear test: selection SPF grades ICR mouse 50 is randomly divided into 5 groups, every group 10, male and female are each Half.Test group intragastric administration on mice gives tested material (tested material in embodiment 6), and high, medium and low dosage is respectively 10.0,5.0, 2.5g/kg BW；Negative control group (distilled water)；Positive controls (cyclophosphamide 40mg/kg BW).Using 2 oral stomach-fillings Method is tested, and interval is for 24 hours.6h is put to death after the 2nd stomach-filling with cervical dislocation；Femur is taken, it is fixed, it dyes, counts, calculate PCE/NCE value.Mouse inbred strain: selection bull SPF grades ICR mouse 50 is randomly divided into 5 groups, every group 10. The group design of test is identical as micronucleus test.Daily stomach-filling 1 time, continuous 5d, 35d is after last stomach-filling with cervical vertebra Dislocation method is put to death；Epididymis is taken to shred, smear is dry, with 1.5% eosin stains after methanol is fixed, counts, and calculates defective sperm hair Raw rate.

Experimental result: each dosage group mouse of the tested product of elder jelly, polychromatic erythrocyte Rate of Appearance Micronuclei and feminine gender Control group compares, and is statistically no significant difference (P > 0.05)；And positive controls then show mutagenesis (P < 0.05), illustrate that mouse microkernel test result for feminine gender, has no mutagenesis under each dosage conditions.The tested product of elder jelly Each dosage group mouse sperm deformity rate compared with negative control group, be statistically no significant difference (P > 0.05)；It is positive Control has statistical significant difference (P < 0.05) with negative control, illustrates that sperm shape abnormality test result is feminine gender, to small Mouse sperm is acted on without aberration inducing.

Embodiment 8:

Rat 30d feeding trial: selection SPF grades Wistar rat 80 is randomly divided into 4 groups, every group 20, male and female are each Half.High dose group is the tested material in embodiment 6；(concentration synthetism rasp berry juice, concentration flores sambuci extracting solution and yeast β-Portugal are poly- Sugar) respectively by 50% of the tested material concentration in embodiment 6 and 25% be added in, in low dose group, remaining supplementary material is by real Apply the tested material concentration addition in example 6, by production method of the present invention it is obtained in, low dose group tested material.Control group (steams Distilled water).Daily stomach-filling 1 time, continuous 30d, gives volume 1g/100g BW.Observed during test the general performance of animal, behavior, Poisoning symptom and death condition, weighing calculate intake, food week utilization rate and overall utilization.It weighed in test the 31st day, fiber crops Liquor-saturated, inferior caval vein blood sampling carries out hematological indices detection and blood parameters detection, and dissection animal taking internal organ weighs and does disease Manage histological examination.

Experimental result: groups of animals well-grown, weight are by Zhou Zengchang, diet, behavioral activity, mode of appearance during test Etc. being showed no exception.It has no toxic reaction, and occurs without death.Weight, food-intake, food are sharp weekly for each sample dosage group animal Compared with the control group with each index of rate, difference is not statistically significant (P > 0.05).The white blood cell count(WBC) of each dosage group rat, The routine blood indexes such as red blood cell count(RBC), hemoglobin concentration, lymphocyte ratio, monocyte ratio, glutamic-pyruvic transaminase, millet straw The blood biochemistry index such as transaminase, urea nitrogen, creatinine, blood glucose, seralbumin, total protein, total cholesterol, triglycerides, on an empty stomach Weight, internal organs weight, also there was no significant difference (P > 0.05) for dirty body ratio.Rats Organs and Tissues absolute weight and relative weight (dirty/body ratio Value), tissue pathology checking's (liver,kidney,spleen, Stomach duodenum, testis or ovary) there are no exception.

Embodiment 9:

The zoopery of elder jelly preventing cold: SPF grades BALB/c mouse 60 are chosen, is randomly divided into 5 groups, every group 12, half male and half female.Oseltamivir phosphate particle group (9.67mg/ (kgd)), " Zhengcaihu Drink " group (1933mg/ (kg ) and elder jelly group (sample of embodiment 2,20g/ (kgd)) d).Volume 20g/kg BW is given, it is 1 time a day, normal right Equal amount of distilled water stomach-filling is given according to group and model control group.Continuous gavage 30d.After 30d, in addition to Normal group, remaining each group Mouse ether light anesthesia, with the infection of 50LD50 influenza virus drop nose, every 50 μ l, Normal group is with the sterile life of equivalent Manage salt water collunarium.The 5th day, 12h fasting for solids and liquids after administration after infection, mouse weight is weighed, cervical dislocation puts to death mouse, and dissection is plucked Full lung is taken, it is clean with brine, surface residual moisture is blotted with filter paper, calculates Lung Exponent.Lung Exponent=lung quality (g)/body quality (g) * 100%.Lung index=(model group Lung Exponent mean value-administration group Lung Exponent mean value)/model Group Lung Exponent mean value * 100%.Using SPSS19.0 statistical software, measurement data uses mean ± standard deviation (mean ± SD) table Show, uses independent samples t test.

Experimental result: seen from table 3, compared with Normal group, significant difference (P < 0.01).Though each group weight is relatively made It is light before mould, but decline is unobvious, and compared with model control group, there were significant differences.Wherein effectiveness is successively phosphoric acid Austria Si Tawei particle group, " Zhengcaihu Drink " group, elder jelly group.After intragastric administration on mice elder jelly 28d, with influenza virus sense Mouse is contaminated, the anti-infection ability of mouse can be improved in elder jelly, and has the function of reducing Lung Exponent.

The results of animal (mean ± SD) of the 3 anti-flu of elder jelly of table

Note: compared with Normal group, ▲ ▲ P < 0.01.Compared with model control group, △ △ P < 0.01, △ P < 0.05。

Embodiment 10:

Influence of the elder jelly to immune function of mice: SPF grades of male BALB/c mouses are chosen, are randomly divided into 4 groups, often Group 10.High dose group (sample of embodiment 2) stomach-filling daily 2 times, each 0.4g；Middle dose group (sample of embodiment 2) is every Its stomach-filling 1 time, each 0.4g；Low dose group (sample of embodiment 2) stomach-filling daily 1 time, each 0.2g elder jelly；Control Group daily stomach-filling 1 time, each 0.4g distilled water.Continuous gavage 30d.Referring to " health food is examined and assessment technique enforcement of regulations Handbook " in strengthen immunity functional check method, carry out the mouse spleen lymphocyte conversion test of ConA induction, mouse delayed Allergy test and NK cytoactive detection.

Experimental result:

Influence of the elder jelly to mouse cell immune function: mice organs/weight ratio is in low, middle and high dose groups With compare comparison among groups there are no significant difference (P > 0.05), illustrate that elder jelly does not make significant difference to spleen weight.By Table 4 as it can be seen that compared with the control group, middle and high dosage group has the proliferation (P < 0.05, P < 0.01) for remarkably promoting splenic lymphocytes, and There was no significant difference for low dose group (P > 0.05), illustrates that elder jelly significantly improves spleen lymphocyte proliferation ability.With Control group is compared, and the swelling degree of the paw of middle and high dosage group mouse increases obvious (P < 0.05, P < 0.01), and low dose group is without aobvious It writes sex differernce (P > 0.05), it is seen that elder jelly can improve animal delayed allergy.Elder jelly is thin to mouse NK The influence of cytoactive: by table 5 as it can be seen that various dose group is administered mouse, each group dosage group compared with the control group, NK cell Activity rate has that there were significant differences, can enhance the NK cell activity of mouse.After elder jelly continuous gavage 30d, to mouse Test for celluar immunity and NK cells in mice test result are positive, and meet " health food is examined and assessment technique specification " The requirement of enhancing body immunity in (2003) illustrates that elder jelly has the work of immunity function in enhancing Mice Body With.

Influence (mean ± SD) of the 4 elder jelly of table to mouse cell immune function

Note: compared with the control group, ▲ ▲ P < 0.01, ▲ P < 0.05.

5 elder pudding of table influence (mean ± SD) active on NK cells in mice

Note: compared with the control group, ▲ ▲ P < 0.01, ▲ P < 0.05.

The preferred embodiment of the present invention has been described in detail above.It should be appreciated that the ordinary skill of this field is without wound The property made labour, which according to the present invention can conceive, makes many modifications and variations.Therefore, all technician in the art Pass through the available technology of logical analysis, reasoning, or a limited experiment on the basis of existing technology under this invention's idea Scheme, all should be within the scope of protection determined by the claims.

Claims

1. a kind of preparation method of elder jelly, which comprises the steps of:

(1) prepare synthetism rasp berry inspissated juice: elder berry is crushed to obtain slurries, by maceraring enzyme be added in the slurries into Row enzymatic hydrolysis, filtering obtain the first extracting solution and slag, and RO water is added in Xiang Suoshu slag and obtains slag liquid, the slag and the RO water Ratio is 1:3~4, extracts 0.5~1h under conditions of 40~55 DEG C to the slag liquid, the second extracting solution is obtained by filtration, and is merged First extracting solution and the second extracting solution obtain synthetism rasp berry juice, are centrifuged to obtain the first supernatant to the synthetism rasp berry juice, by institute It states the first supernatant and obtains permeate by ultrafiltration membrane, the permeate is concentrated into soluble solid 25~30%, then Sterilize to obtain the synthetism rasp berry inspissated juice under 400-600 MPa pressure；

(2) it prepares flores sambuci inspissated juice: crushing flores sambuci, smashed flores sambuci is extracted to obtain third extraction Liquid is centrifuged to obtain the second supernatant to the third extracting solution, obtains filtered solution to second supernatant liquid filtering, The filtered solution is carried out to be concentrated into soluble solid 15~20%, and then sterilizes and obtains flores sambuci inspissated juice；

(3) change mass: by carragheen, gellan gum, tamarind gum, oligofructose, antierythrite, isomaltoketose, trichlorine sugarcane Sugar and yeast beta-dextran are uniformly mixed so as to obtain glycocoll mixture, after room temperature RO water is added in Xiang Suoshu glycocoll mixture, through colloid mill It is put into after milled and boils material pot, boiled material to abundant dissolution and obtain feed liquid, the feed liquid is then cooled to 85~95 DEG C；

(4) ingredient: dissolving calcium lactate with 80~90 DEG C of RO water, dissolved calcium lactate be added in the feed liquid of step (3), every After at least 1min, the synthetism rasp berry inspissated juice of step (1) and the flores sambuci inspissated juice of step (2) is added, after at least 1min, The additive of 80~90 DEG C of 5.9~6.8 parts of RO water dissolutions is added, obtains semi-finished product solution, detects the semi-finished product solution Color, flavor, mouthfeel, pH, soluble solid and feed liquid forming degree, when the soluble solid 18.5 of the semi-finished product solution ± 0.2%, pH is 3.7 ± 0.1, flavor is synthetism rasp berry juice flavor, color is aubergine, and the semi-finished product solution is qualification；

(5) filling: filtering and encapsulating while hot being carried out to qualified semi-finished product solution, filled the semi-finished product solution with filling machine To jelly package, at 73-75 DEG C, the control of each batch of material is filled in 40min for filling temperature control, it is filling after the completion of immediately Sterilizing；

(6) sterilize: sterilising temp is 80~85 DEG C, 15~20min of sterilization time, puts into cooling in cold water after sterilizing immediately, cold But the water temperature in pond is controlled at 20 DEG C hereinafter, be no less than 15min cooling time, is cooled to room temperature；

(7) dry: drying temperature is not higher than 40 DEG C, and the elder jelly is made.

2. preparation method as described in claim 1, wherein the temperature of the enzymatic hydrolysis in step (1) is 45~55 DEG C, enzymolysis time For 1~2h.

3. preparation method as described in claim 1, wherein the additive amount of the maceraring enzyme in step (1) be fruit weight 0.05~ 0.38%.

4. preparation method as claimed in claim 3, wherein the dosage of the maceraring enzyme is the 0.05-0.1% of fruit weight.

5. preparation method as claimed in claim 3, wherein the composition ratio of the maceraring enzyme are as follows: pectase 6000-8000 μ/ G, cellulase 400-800 μ/g, zytase 2000-3000 μ/g.

6. preparation method as described in claim 1, wherein the process of the extraction in step (2) is to smashed elder Spend middle addition RO water, the ratio of the smashed flores sambuci and the RO water is 1:12~15, citric acid is added, according to weight Score ratio is measured, the additive amount of the citric acid is the 2% of RO water, under conditions of 250~300MPa, 5~15min of pressure maintaining.

7. preparation method as described in claim 1, wherein the temperature of the sterilizing in step (2) is 130-135 DEG C, when sterilizing Between be 5-15s.

8. preparation method as described in claim 1, wherein the additive in step (4), including citric acid and sodium citrate.

9. a kind of elder jelly, which is characterized in that according to weight fraction meter, including 13~25 parts of synthetism rasp berry inspissated juice, connect Bone wood 4.5~6.5 parts of inspissated juice of flower, 0.25~0.4 part of carragheen, 0.08~0.12 part of gellan gum, tamarind gum 0.11~ 0.14 part, 3.2~7.0 parts of oligofructose, 3.0~7.0 parts of antierythrite, 2.9~4.5 parts of isomaltoketose, Sucralose 0.02~0.025 part, 0.15~0.25 part of yeast beta-dextran, 0.25~0.35 part of citric acid, sodium citrate 0.20~0.25 Part and 0.25~0.32 part of L-calcium lactate.

10. elder jelly as claimed in claim 9, wherein specific retention is 79.4~83.9%, and procyanidins content is 1.35~1.68mg/g.