CN112690450A - Composition for relieving alcoholism and protecting liver, preparation method thereof and product containing composition - Google Patents
Composition for relieving alcoholism and protecting liver, preparation method thereof and product containing composition Download PDFInfo
- Publication number
- CN112690450A CN112690450A CN202011520581.3A CN202011520581A CN112690450A CN 112690450 A CN112690450 A CN 112690450A CN 202011520581 A CN202011520581 A CN 202011520581A CN 112690450 A CN112690450 A CN 112690450A
- Authority
- CN
- China
- Prior art keywords
- powder
- composition
- preparation
- extract
- parts
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000000203 mixture Substances 0.000 title claims abstract description 110
- 210000004185 liver Anatomy 0.000 title claims abstract description 55
- 238000002360 preparation method Methods 0.000 title claims abstract description 46
- 208000007848 Alcoholism Diseases 0.000 title claims abstract description 37
- 201000007930 alcohol dependence Diseases 0.000 title claims abstract description 37
- 230000000694 effects Effects 0.000 claims abstract description 81
- 244000010000 Hovenia dulcis Species 0.000 claims abstract description 46
- 235000008584 Hovenia dulcis Nutrition 0.000 claims abstract description 46
- 240000008042 Zea mays Species 0.000 claims abstract description 36
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 36
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 36
- 235000005822 corn Nutrition 0.000 claims abstract description 36
- 102000015636 Oligopeptides Human genes 0.000 claims abstract description 34
- 108010038807 Oligopeptides Proteins 0.000 claims abstract description 34
- 235000021466 carotenoid Nutrition 0.000 claims abstract description 34
- 150000001747 carotenoids Chemical class 0.000 claims abstract description 31
- 239000000284 extract Substances 0.000 claims abstract description 27
- 229940052016 turmeric extract Drugs 0.000 claims abstract description 10
- 235000020240 turmeric extract Nutrition 0.000 claims abstract description 10
- 239000008513 turmeric extract Substances 0.000 claims abstract description 10
- 239000000843 powder Substances 0.000 claims description 177
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 148
- 244000046146 Pueraria lobata Species 0.000 claims description 45
- 235000010575 Pueraria lobata Nutrition 0.000 claims description 45
- KJXSIXMJHKAJOD-LSDHHAIUSA-N (+)-dihydromyricetin Chemical compound C1([C@@H]2[C@H](C(C3=C(O)C=C(O)C=C3O2)=O)O)=CC(O)=C(O)C(O)=C1 KJXSIXMJHKAJOD-LSDHHAIUSA-N 0.000 claims description 40
- 238000000034 method Methods 0.000 claims description 34
- RXUWDKBZZLIASQ-UHFFFAOYSA-N Puerarin Natural products OCC1OC(Oc2c(O)cc(O)c3C(=O)C(=COc23)c4ccc(O)cc4)C(O)C(O)C1O RXUWDKBZZLIASQ-UHFFFAOYSA-N 0.000 claims description 29
- HKEAFJYKMMKDOR-VPRICQMDSA-N puerarin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1C1=C(O)C=CC(C2=O)=C1OC=C2C1=CC=C(O)C=C1 HKEAFJYKMMKDOR-VPRICQMDSA-N 0.000 claims description 29
- 235000012680 lutein Nutrition 0.000 claims description 22
- 239000001656 lutein Substances 0.000 claims description 22
- KBPHJBAIARWVSC-RGZFRNHPSA-N lutein Chemical compound C([C@H](O)CC=1C)C(C)(C)C=1\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\[C@H]1C(C)=C[C@H](O)CC1(C)C KBPHJBAIARWVSC-RGZFRNHPSA-N 0.000 claims description 22
- 229960005375 lutein Drugs 0.000 claims description 22
- ORAKUVXRZWMARG-WZLJTJAWSA-N lutein Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CCCC1(C)C)C=CC=C(/C)C=CC2C(=CC(O)CC2(C)C)C ORAKUVXRZWMARG-WZLJTJAWSA-N 0.000 claims description 22
- KBPHJBAIARWVSC-XQIHNALSSA-N trans-lutein Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CC(O)CC1(C)C)C=CC=C(/C)C=CC2C(=CC(O)CC2(C)C)C KBPHJBAIARWVSC-XQIHNALSSA-N 0.000 claims description 22
- FJHBOVDFOQMZRV-XQIHNALSSA-N xanthophyll Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CC(O)CC1(C)C)C=CC=C(/C)C=CC2C=C(C)C(O)CC2(C)C FJHBOVDFOQMZRV-XQIHNALSSA-N 0.000 claims description 22
- 244000163122 Curcuma domestica Species 0.000 claims description 20
- KQILIWXGGKGKNX-UHFFFAOYSA-N dihydromyricetin Natural products OC1C(=C(Oc2cc(O)cc(O)c12)c3cc(O)c(O)c(O)c3)O KQILIWXGGKGKNX-UHFFFAOYSA-N 0.000 claims description 20
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 19
- 235000003392 Curcuma domestica Nutrition 0.000 claims description 17
- 235000003373 curcuma longa Nutrition 0.000 claims description 17
- 239000003094 microcapsule Substances 0.000 claims description 17
- 235000013976 turmeric Nutrition 0.000 claims description 17
- 238000003908 quality control method Methods 0.000 claims description 16
- 235000013406 prebiotics Nutrition 0.000 claims description 13
- 210000000582 semen Anatomy 0.000 claims description 13
- 229930003944 flavone Natural products 0.000 claims description 12
- 235000011949 flavones Nutrition 0.000 claims description 12
- 206010019133 Hangover Diseases 0.000 claims description 11
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 claims description 11
- 150000002212 flavone derivatives Chemical class 0.000 claims description 11
- 238000002156 mixing Methods 0.000 claims description 11
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 claims description 11
- VFLDPWHFBUODDF-FCXRPNKRSA-N curcumin Chemical compound C1=C(O)C(OC)=CC(\C=C\C(=O)CC(=O)\C=C\C=2C=C(OC)C(O)=CC=2)=C1 VFLDPWHFBUODDF-FCXRPNKRSA-N 0.000 claims description 10
- NVNLLIYOARQCIX-MSHCCFNRSA-N Nisin Chemical compound N1C(=O)[C@@H](CC(C)C)NC(=O)C(=C)NC(=O)[C@@H]([C@H](C)CC)NC(=O)[C@@H](NC(=O)C(=C/C)/NC(=O)[C@H](N)[C@H](C)CC)CSC[C@@H]1C(=O)N[C@@H]1C(=O)N2CCC[C@@H]2C(=O)NCC(=O)N[C@@H](C(=O)N[C@H](CCCCN)C(=O)N[C@@H]2C(NCC(=O)N[C@H](C)C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCSC)C(=O)NCC(=O)N[C@H](CS[C@@H]2C)C(=O)N[C@H](CC(N)=O)C(=O)N[C@H](CCSC)C(=O)N[C@H](CCCCN)C(=O)N[C@@H]2C(N[C@H](C)C(=O)N[C@@H]3C(=O)N[C@@H](C(N[C@H](CC=4NC=NC=4)C(=O)N[C@H](CS[C@@H]3C)C(=O)N[C@H](CO)C(=O)N[C@H]([C@H](C)CC)C(=O)N[C@H](CC=3NC=NC=3)C(=O)N[C@H](C(C)C)C(=O)NC(=C)C(=O)N[C@H](CCCCN)C(O)=O)=O)CS[C@@H]2C)=O)=O)CS[C@@H]1C NVNLLIYOARQCIX-MSHCCFNRSA-N 0.000 claims description 9
- 108010053775 Nisin Proteins 0.000 claims description 9
- 240000006365 Vitis vinifera Species 0.000 claims description 9
- 235000014787 Vitis vinifera Nutrition 0.000 claims description 9
- 238000002835 absorbance Methods 0.000 claims description 9
- 238000004811 liquid chromatography Methods 0.000 claims description 9
- 239000004309 nisin Substances 0.000 claims description 9
- 235000010297 nisin Nutrition 0.000 claims description 9
- 238000001694 spray drying Methods 0.000 claims description 9
- 235000004936 Bromus mango Nutrition 0.000 claims description 8
- 240000007228 Mangifera indica Species 0.000 claims description 8
- 235000014826 Mangifera indica Nutrition 0.000 claims description 8
- 235000009184 Spondias indica Nutrition 0.000 claims description 8
- 238000000605 extraction Methods 0.000 claims description 8
- FTSSQIKWUOOEGC-RULYVFMPSA-N fructooligosaccharide Chemical compound OC[C@H]1O[C@@](CO)(OC[C@@]2(OC[C@@]3(OC[C@@]4(OC[C@@]5(OC[C@@]6(OC[C@@]7(OC[C@@]8(OC[C@@]9(OC[C@@]%10(OC[C@@]%11(O[C@H]%12O[C@H](CO)[C@@H](O)[C@H](O)[C@H]%12O)O[C@H](CO)[C@@H](O)[C@@H]%11O)O[C@H](CO)[C@@H](O)[C@@H]%10O)O[C@H](CO)[C@@H](O)[C@@H]9O)O[C@H](CO)[C@@H](O)[C@@H]8O)O[C@H](CO)[C@@H](O)[C@@H]7O)O[C@H](CO)[C@@H](O)[C@@H]6O)O[C@H](CO)[C@@H](O)[C@@H]5O)O[C@H](CO)[C@@H](O)[C@@H]4O)O[C@H](CO)[C@@H](O)[C@@H]3O)O[C@H](CO)[C@@H](O)[C@@H]2O)[C@@H](O)[C@@H]1O FTSSQIKWUOOEGC-RULYVFMPSA-N 0.000 claims description 6
- 229940107187 fructooligosaccharide Drugs 0.000 claims description 6
- 239000005452 food preservative Substances 0.000 claims description 5
- 235000019249 food preservative Nutrition 0.000 claims description 5
- AFSDNFLWKVMVRB-UHFFFAOYSA-N Ellagic acid Chemical compound OC1=C(O)C(OC2=O)=C3C4=C2C=C(O)C(O)=C4OC(=O)C3=C1 AFSDNFLWKVMVRB-UHFFFAOYSA-N 0.000 claims description 4
- 238000005119 centrifugation Methods 0.000 claims description 4
- 235000021255 galacto-oligosaccharides Nutrition 0.000 claims description 4
- 150000003271 galactooligosaccharides Chemical class 0.000 claims description 4
- 229920001542 oligosaccharide Polymers 0.000 claims description 4
- 150000002482 oligosaccharides Chemical class 0.000 claims description 4
- 235000013525 pomegranate juice Nutrition 0.000 claims description 4
- 235000010469 Glycine max Nutrition 0.000 claims description 3
- 229920001202 Inulin Polymers 0.000 claims description 3
- 244000288157 Passiflora edulis Species 0.000 claims description 3
- 235000000370 Passiflora edulis Nutrition 0.000 claims description 3
- FYGDTMLNYKFZSV-DZOUCCHMSA-N alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->4)-D-Glcp Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)O[C@H](O[C@@H]2[C@H](OC(O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-DZOUCCHMSA-N 0.000 claims description 3
- 235000020379 cucumber juice Nutrition 0.000 claims description 3
- HEBKCHPVOIAQTA-NGQZWQHPSA-N d-xylitol Chemical compound OC[C@H](O)C(O)[C@H](O)CO HEBKCHPVOIAQTA-NGQZWQHPSA-N 0.000 claims description 3
- 235000019674 grape juice Nutrition 0.000 claims description 3
- JYJIGFIDKWBXDU-MNNPPOADSA-N inulin Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)OC[C@]1(OC[C@]2(OC[C@]3(OC[C@]4(OC[C@]5(OC[C@]6(OC[C@]7(OC[C@]8(OC[C@]9(OC[C@]%10(OC[C@]%11(OC[C@]%12(OC[C@]%13(OC[C@]%14(OC[C@]%15(OC[C@]%16(OC[C@]%17(OC[C@]%18(OC[C@]%19(OC[C@]%20(OC[C@]%21(OC[C@]%22(OC[C@]%23(OC[C@]%24(OC[C@]%25(OC[C@]%26(OC[C@]%27(OC[C@]%28(OC[C@]%29(OC[C@]%30(OC[C@]%31(OC[C@]%32(OC[C@]%33(OC[C@]%34(OC[C@]%35(OC[C@]%36(O[C@@H]%37[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O%37)O)[C@H]([C@H](O)[C@@H](CO)O%36)O)[C@H]([C@H](O)[C@@H](CO)O%35)O)[C@H]([C@H](O)[C@@H](CO)O%34)O)[C@H]([C@H](O)[C@@H](CO)O%33)O)[C@H]([C@H](O)[C@@H](CO)O%32)O)[C@H]([C@H](O)[C@@H](CO)O%31)O)[C@H]([C@H](O)[C@@H](CO)O%30)O)[C@H]([C@H](O)[C@@H](CO)O%29)O)[C@H]([C@H](O)[C@@H](CO)O%28)O)[C@H]([C@H](O)[C@@H](CO)O%27)O)[C@H]([C@H](O)[C@@H](CO)O%26)O)[C@H]([C@H](O)[C@@H](CO)O%25)O)[C@H]([C@H](O)[C@@H](CO)O%24)O)[C@H]([C@H](O)[C@@H](CO)O%23)O)[C@H]([C@H](O)[C@@H](CO)O%22)O)[C@H]([C@H](O)[C@@H](CO)O%21)O)[C@H]([C@H](O)[C@@H](CO)O%20)O)[C@H]([C@H](O)[C@@H](CO)O%19)O)[C@H]([C@H](O)[C@@H](CO)O%18)O)[C@H]([C@H](O)[C@@H](CO)O%17)O)[C@H]([C@H](O)[C@@H](CO)O%16)O)[C@H]([C@H](O)[C@@H](CO)O%15)O)[C@H]([C@H](O)[C@@H](CO)O%14)O)[C@H]([C@H](O)[C@@H](CO)O%13)O)[C@H]([C@H](O)[C@@H](CO)O%12)O)[C@H]([C@H](O)[C@@H](CO)O%11)O)[C@H]([C@H](O)[C@@H](CO)O%10)O)[C@H]([C@H](O)[C@@H](CO)O9)O)[C@H]([C@H](O)[C@@H](CO)O8)O)[C@H]([C@H](O)[C@@H](CO)O7)O)[C@H]([C@H](O)[C@@H](CO)O6)O)[C@H]([C@H](O)[C@@H](CO)O5)O)[C@H]([C@H](O)[C@@H](CO)O4)O)[C@H]([C@H](O)[C@@H](CO)O3)O)[C@H]([C@H](O)[C@@H](CO)O2)O)[C@@H](O)[C@H](O)[C@@H](CO)O1 JYJIGFIDKWBXDU-MNNPPOADSA-N 0.000 claims description 3
- 229940029339 inulin Drugs 0.000 claims description 3
- DLRVVLDZNNYCBX-RTPHMHGBSA-N isomaltose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)C(O)O1 DLRVVLDZNNYCBX-RTPHMHGBSA-N 0.000 claims description 3
- 235000014375 Curcuma Nutrition 0.000 claims description 2
- 230000003472 neutralizing effect Effects 0.000 claims 2
- 230000002443 hepatoprotective effect Effects 0.000 claims 1
- 208000024891 symptom Diseases 0.000 abstract description 12
- 230000002195 synergetic effect Effects 0.000 abstract description 6
- 239000009262 Puerariae Radix plant extract Substances 0.000 abstract description 3
- 235000019441 ethanol Nutrition 0.000 description 80
- 239000000047 product Substances 0.000 description 68
- 230000035622 drinking Effects 0.000 description 50
- 239000000243 solution Substances 0.000 description 45
- 241000699670 Mus sp. Species 0.000 description 30
- 238000001914 filtration Methods 0.000 description 23
- 210000004369 blood Anatomy 0.000 description 19
- 239000008280 blood Substances 0.000 description 19
- 210000002966 serum Anatomy 0.000 description 18
- 239000000706 filtrate Substances 0.000 description 16
- 239000000463 material Substances 0.000 description 15
- 238000012360 testing method Methods 0.000 description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 15
- 235000013361 beverage Nutrition 0.000 description 14
- 108010021809 Alcohol dehydrogenase Proteins 0.000 description 13
- 238000005303 weighing Methods 0.000 description 13
- 235000014101 wine Nutrition 0.000 description 12
- 239000007788 liquid Substances 0.000 description 10
- 238000004519 manufacturing process Methods 0.000 description 10
- 239000000523 sample Substances 0.000 description 10
- 239000012085 test solution Substances 0.000 description 10
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 9
- 239000003814 drug Substances 0.000 description 9
- 238000004128 high performance liquid chromatography Methods 0.000 description 9
- 238000007873 sieving Methods 0.000 description 9
- 102100036475 Alanine aminotransferase 1 Human genes 0.000 description 8
- 108010082126 Alanine transaminase Proteins 0.000 description 8
- 241000699666 Mus <mouse, genus> Species 0.000 description 8
- 238000010521 absorption reaction Methods 0.000 description 8
- 238000000926 separation method Methods 0.000 description 8
- 210000002784 stomach Anatomy 0.000 description 8
- 108010003415 Aspartate Aminotransferases Proteins 0.000 description 7
- 102000004625 Aspartate Aminotransferases Human genes 0.000 description 7
- 241000219780 Pueraria Species 0.000 description 7
- 239000002775 capsule Substances 0.000 description 7
- 238000001816 cooling Methods 0.000 description 7
- 229930003935 flavonoid Natural products 0.000 description 7
- 150000002215 flavonoids Chemical class 0.000 description 7
- 235000017173 flavonoids Nutrition 0.000 description 7
- 210000001035 gastrointestinal tract Anatomy 0.000 description 7
- 230000004060 metabolic process Effects 0.000 description 7
- -1 oxygen free radical Chemical class 0.000 description 7
- 239000002245 particle Substances 0.000 description 7
- 239000013558 reference substance Substances 0.000 description 7
- 238000003756 stirring Methods 0.000 description 7
- 206010006326 Breath odour Diseases 0.000 description 6
- 244000303040 Glycyrrhiza glabra Species 0.000 description 6
- 235000006200 Glycyrrhiza glabra Nutrition 0.000 description 6
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 230000001476 alcoholic effect Effects 0.000 description 6
- 238000001514 detection method Methods 0.000 description 6
- 230000006870 function Effects 0.000 description 6
- 230000003647 oxidation Effects 0.000 description 6
- 238000007254 oxidation reaction Methods 0.000 description 6
- 230000001737 promoting effect Effects 0.000 description 6
- 235000018102 proteins Nutrition 0.000 description 6
- 102000004169 proteins and genes Human genes 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- 230000002829 reductive effect Effects 0.000 description 6
- 238000003860 storage Methods 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 102000007698 Alcohol dehydrogenase Human genes 0.000 description 5
- 241001465754 Metazoa Species 0.000 description 5
- 235000001014 amino acid Nutrition 0.000 description 5
- 229940024606 amino acid Drugs 0.000 description 5
- 150000001413 amino acids Chemical class 0.000 description 5
- 230000000052 comparative effect Effects 0.000 description 5
- 239000002552 dosage form Substances 0.000 description 5
- 238000001035 drying Methods 0.000 description 5
- 230000007937 eating Effects 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 239000000945 filler Substances 0.000 description 5
- 238000011049 filling Methods 0.000 description 5
- 239000000796 flavoring agent Substances 0.000 description 5
- 235000019634 flavors Nutrition 0.000 description 5
- 235000013305 food Nutrition 0.000 description 5
- 230000002496 gastric effect Effects 0.000 description 5
- 230000036541 health Effects 0.000 description 5
- 238000010438 heat treatment Methods 0.000 description 5
- 239000001301 oxygen Substances 0.000 description 5
- 229910052760 oxygen Inorganic materials 0.000 description 5
- 150000003254 radicals Chemical class 0.000 description 5
- 230000001603 reducing effect Effects 0.000 description 5
- 239000012088 reference solution Substances 0.000 description 5
- 239000012488 sample solution Substances 0.000 description 5
- 239000007921 spray Substances 0.000 description 5
- 206010067125 Liver injury Diseases 0.000 description 4
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 4
- 108010081577 aldehyde dehydrogenase (NAD(P)+) Proteins 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 230000001684 chronic effect Effects 0.000 description 4
- 238000004140 cleaning Methods 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 4
- 235000009508 confectionery Nutrition 0.000 description 4
- 235000012754 curcumin Nutrition 0.000 description 4
- 239000004148 curcumin Substances 0.000 description 4
- 229940109262 curcumin Drugs 0.000 description 4
- VFLDPWHFBUODDF-UHFFFAOYSA-N diferuloylmethane Natural products C1=C(O)C(OC)=CC(C=CC(=O)CC(=O)C=CC=2C=C(OC)C(O)=CC=2)=C1 VFLDPWHFBUODDF-UHFFFAOYSA-N 0.000 description 4
- 208000002173 dizziness Diseases 0.000 description 4
- 206010016256 fatigue Diseases 0.000 description 4
- 235000015203 fruit juice Nutrition 0.000 description 4
- 238000003304 gavage Methods 0.000 description 4
- LPLVUJXQOOQHMX-QWBHMCJMSA-N glycyrrhizinic acid Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@H](O[C@@H]1O[C@@H]1C([C@H]2[C@]([C@@H]3[C@@]([C@@]4(CC[C@@]5(C)CC[C@@](C)(C[C@H]5C4=CC3=O)C(O)=O)C)(C)CC2)(C)CC1)(C)C)C(O)=O)[C@@H]1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O LPLVUJXQOOQHMX-QWBHMCJMSA-N 0.000 description 4
- 239000008187 granular material Substances 0.000 description 4
- 231100000753 hepatic injury Toxicity 0.000 description 4
- 241000411851 herbal medicine Species 0.000 description 4
- 230000001965 increasing effect Effects 0.000 description 4
- 235000011477 liquorice Nutrition 0.000 description 4
- YTJSFYQNRXLOIC-UHFFFAOYSA-N octadecylsilane Chemical compound CCCCCCCCCCCCCCCCCC[SiH3] YTJSFYQNRXLOIC-UHFFFAOYSA-N 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 108090000765 processed proteins & peptides Proteins 0.000 description 4
- 239000002994 raw material Substances 0.000 description 4
- 230000001105 regulatory effect Effects 0.000 description 4
- 239000000377 silicon dioxide Substances 0.000 description 4
- 210000001519 tissue Anatomy 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 241000186000 Bifidobacterium Species 0.000 description 3
- ZQSIJRDFPHDXIC-UHFFFAOYSA-N Daidzein Natural products C1=CC(O)=CC=C1C1=COC2=CC(O)=CC=C2C1=O ZQSIJRDFPHDXIC-UHFFFAOYSA-N 0.000 description 3
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical class OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 3
- 206010019233 Headaches Diseases 0.000 description 3
- 208000032140 Sleepiness Diseases 0.000 description 3
- 206010041349 Somnolence Diseases 0.000 description 3
- 108090000340 Transaminases Proteins 0.000 description 3
- 206010047700 Vomiting Diseases 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 230000002075 anti-alcohol Effects 0.000 description 3
- 238000003556 assay Methods 0.000 description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- 238000004587 chromatography analysis Methods 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 235000013399 edible fruits Nutrition 0.000 description 3
- 230000002349 favourable effect Effects 0.000 description 3
- 231100000869 headache Toxicity 0.000 description 3
- 235000015110 jellies Nutrition 0.000 description 3
- 239000008274 jelly Substances 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- 231100000252 nontoxic Toxicity 0.000 description 3
- 230000003000 nontoxic effect Effects 0.000 description 3
- 102000004196 processed proteins & peptides Human genes 0.000 description 3
- 230000037321 sleepiness Effects 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- 230000035922 thirst Effects 0.000 description 3
- 238000004704 ultra performance liquid chromatography Methods 0.000 description 3
- UCTWMZQNUQWSLP-VIFPVBQESA-N (R)-adrenaline Chemical compound CNC[C@H](O)C1=CC=C(O)C(O)=C1 UCTWMZQNUQWSLP-VIFPVBQESA-N 0.000 description 2
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 2
- IKHGUXGNUITLKF-UHFFFAOYSA-N Acetaldehyde Chemical compound CC=O IKHGUXGNUITLKF-UHFFFAOYSA-N 0.000 description 2
- 238000011740 C57BL/6 mouse Methods 0.000 description 2
- 108090000317 Chymotrypsin Proteins 0.000 description 2
- 206010010774 Constipation Diseases 0.000 description 2
- 230000005526 G1 to G0 transition Effects 0.000 description 2
- 244000068988 Glycine max Species 0.000 description 2
- 235000001453 Glycyrrhiza echinata Nutrition 0.000 description 2
- 235000017382 Glycyrrhiza lepidota Nutrition 0.000 description 2
- 229920000569 Gum karaya Polymers 0.000 description 2
- 235000008586 Hovenia Nutrition 0.000 description 2
- 241000405398 Hovenia Species 0.000 description 2
- 206010020772 Hypertension Diseases 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 2
- 108010009736 Protein Hydrolysates Proteins 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 241000934878 Sterculia Species 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000003078 antioxidant effect Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000017531 blood circulation Effects 0.000 description 2
- 238000009835 boiling Methods 0.000 description 2
- 150000001746 carotenes Chemical class 0.000 description 2
- 235000005473 carotenes Nutrition 0.000 description 2
- 229960002376 chymotrypsin Drugs 0.000 description 2
- 239000012141 concentrate Substances 0.000 description 2
- 238000000354 decomposition reaction Methods 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 238000010828 elution Methods 0.000 description 2
- 230000001804 emulsifying effect Effects 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 210000001508 eye Anatomy 0.000 description 2
- 235000020828 fasting Nutrition 0.000 description 2
- 238000010579 first pass effect Methods 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 210000003736 gastrointestinal content Anatomy 0.000 description 2
- 238000005469 granulation Methods 0.000 description 2
- 230000003179 granulation Effects 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 238000009776 industrial production Methods 0.000 description 2
- 239000000231 karaya gum Substances 0.000 description 2
- 235000010494 karaya gum Nutrition 0.000 description 2
- 229940039371 karaya gum Drugs 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 229940010454 licorice Drugs 0.000 description 2
- 230000003859 lipid peroxidation Effects 0.000 description 2
- HQKMJHAJHXVSDF-UHFFFAOYSA-L magnesium stearate Chemical compound [Mg+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O HQKMJHAJHXVSDF-UHFFFAOYSA-L 0.000 description 2
- 229910052751 metal Inorganic materials 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- GBMDVOWEEQVZKZ-UHFFFAOYSA-N methanol;hydrate Chemical compound O.OC GBMDVOWEEQVZKZ-UHFFFAOYSA-N 0.000 description 2
- 230000036284 oxygen consumption Effects 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- 239000002504 physiological saline solution Substances 0.000 description 2
- 239000000049 pigment Substances 0.000 description 2
- 150000008442 polyphenolic compounds Chemical class 0.000 description 2
- 235000013824 polyphenols Nutrition 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 239000003531 protein hydrolysate Substances 0.000 description 2
- 238000010791 quenching Methods 0.000 description 2
- 239000003642 reactive oxygen metabolite Substances 0.000 description 2
- 235000020095 red wine Nutrition 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000028527 righting reflex Effects 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 230000000638 stimulation Effects 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 239000003826 tablet Substances 0.000 description 2
- 102000014898 transaminase activity proteins Human genes 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- 235000013343 vitamin Nutrition 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 230000008673 vomiting Effects 0.000 description 2
- 235000020097 white wine Nutrition 0.000 description 2
- UUUHXMGGBIUAPW-UHFFFAOYSA-N 1-[1-[2-[[5-amino-2-[[1-[5-(diaminomethylideneamino)-2-[[1-[3-(1h-indol-3-yl)-2-[(5-oxopyrrolidine-2-carbonyl)amino]propanoyl]pyrrolidine-2-carbonyl]amino]pentanoyl]pyrrolidine-2-carbonyl]amino]-5-oxopentanoyl]amino]-3-methylpentanoyl]pyrrolidine-2-carbon Chemical compound C1CCC(C(=O)N2C(CCC2)C(O)=O)N1C(=O)C(C(C)CC)NC(=O)C(CCC(N)=O)NC(=O)C1CCCN1C(=O)C(CCCN=C(N)N)NC(=O)C1CCCN1C(=O)C(CC=1C2=CC=CC=C2NC=1)NC(=O)C1CCC(=O)N1 UUUHXMGGBIUAPW-UHFFFAOYSA-N 0.000 description 1
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- 235000020927 12-h fasting Nutrition 0.000 description 1
- UCTWMZQNUQWSLP-UHFFFAOYSA-N Adrenaline Natural products CNCC(O)C1=CC=C(O)C(O)=C1 UCTWMZQNUQWSLP-UHFFFAOYSA-N 0.000 description 1
- 229910002012 Aerosil® Inorganic materials 0.000 description 1
- 208000005584 Alcoholic Intoxication Diseases 0.000 description 1
- 206010002091 Anaesthesia Diseases 0.000 description 1
- 206010002383 Angina Pectoris Diseases 0.000 description 1
- 206010003591 Ataxia Diseases 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 229940126062 Compound A Drugs 0.000 description 1
- 206010010947 Coordination abnormal Diseases 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- GMTUGPYJRUMVTC-UHFFFAOYSA-N Daidzin Natural products OC(COc1ccc2C(=O)C(=COc2c1)c3ccc(O)cc3)C(O)C(O)C(O)C=O GMTUGPYJRUMVTC-UHFFFAOYSA-N 0.000 description 1
- KYQZWONCHDNPDP-UHFFFAOYSA-N Daidzoside Natural products OC1C(O)C(O)C(CO)OC1OC1=CC=C2C(=O)C(C=3C=CC(O)=CC=3)=COC2=C1 KYQZWONCHDNPDP-UHFFFAOYSA-N 0.000 description 1
- 101710088194 Dehydrogenase Proteins 0.000 description 1
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 1
- 206010013183 Dislocation of vertebra Diseases 0.000 description 1
- 206010013911 Dysgeusia Diseases 0.000 description 1
- 206010013954 Dysphoria Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000220485 Fabaceae Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
- 229920002907 Guar gum Polymers 0.000 description 1
- 206010019837 Hepatocellular injury Diseases 0.000 description 1
- NLDMNSXOCDLTTB-UHFFFAOYSA-N Heterophylliin A Natural products O1C2COC(=O)C3=CC(O)=C(O)C(O)=C3C3=C(O)C(O)=C(O)C=C3C(=O)OC2C(OC(=O)C=2C=C(O)C(O)=C(O)C=2)C(O)C1OC(=O)C1=CC(O)=C(O)C(O)=C1 NLDMNSXOCDLTTB-UHFFFAOYSA-N 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 235000010955 Hovenia acerba Nutrition 0.000 description 1
- 241001533134 Hovenia acerba Species 0.000 description 1
- 208000031226 Hyperlipidaemia Diseases 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 208000005016 Intestinal Neoplasms Diseases 0.000 description 1
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 1
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 1
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 1
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 1
- 229920000161 Locust bean gum Polymers 0.000 description 1
- 239000005913 Maltodextrin Substances 0.000 description 1
- 229920002774 Maltodextrin Polymers 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- 240000000249 Morus alba Species 0.000 description 1
- 235000008708 Morus alba Nutrition 0.000 description 1
- 206010028836 Neck pain Diseases 0.000 description 1
- 206010034133 Pathogen resistance Diseases 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 102000004270 Peptidyl-Dipeptidase A Human genes 0.000 description 1
- 108090000882 Peptidyl-Dipeptidase A Proteins 0.000 description 1
- 102000003992 Peroxidases Human genes 0.000 description 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 208000001431 Psychomotor Agitation Diseases 0.000 description 1
- 206010037660 Pyrexia Diseases 0.000 description 1
- 206010038743 Restlessness Diseases 0.000 description 1
- 206010038776 Retching Diseases 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 241000219100 Rhamnaceae Species 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 241001424341 Tara spinosa Species 0.000 description 1
- 208000009205 Tinnitus Diseases 0.000 description 1
- 102000003929 Transaminases Human genes 0.000 description 1
- 229930003427 Vitamin E Natural products 0.000 description 1
- IKHGUXGNUITLKF-XPULMUKRSA-N acetaldehyde Chemical compound [14CH]([14CH3])=O IKHGUXGNUITLKF-XPULMUKRSA-N 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 229940102884 adrenalin Drugs 0.000 description 1
- 230000000274 adsorptive effect Effects 0.000 description 1
- 235000004279 alanine Nutrition 0.000 description 1
- 125000003158 alcohol group Chemical group 0.000 description 1
- 229930013930 alkaloid Natural products 0.000 description 1
- 150000003797 alkaloid derivatives Chemical class 0.000 description 1
- OENHQHLEOONYIE-UKMVMLAPSA-N all-trans beta-carotene Natural products CC=1CCCC(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C OENHQHLEOONYIE-UKMVMLAPSA-N 0.000 description 1
- 230000037005 anaesthesia Effects 0.000 description 1
- 239000005557 antagonist Substances 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 230000003542 behavioural effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 235000013734 beta-carotene Nutrition 0.000 description 1
- 239000011648 beta-carotene Substances 0.000 description 1
- TUPZEYHYWIEDIH-WAIFQNFQSA-N beta-carotene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CCCC1(C)C)C=CC=C(/C)C=CC2=CCCCC2(C)C TUPZEYHYWIEDIH-WAIFQNFQSA-N 0.000 description 1
- 229960002747 betacarotene Drugs 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000001851 biosynthetic effect Effects 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 238000010241 blood sampling Methods 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 210000005252 bulbus oculi Anatomy 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000002490 cerebral effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000004087 circulation Effects 0.000 description 1
- 229940052810 complex b Drugs 0.000 description 1
- 230000002508 compound effect Effects 0.000 description 1
- 208000004209 confusion Diseases 0.000 description 1
- 235000007240 daidzein Nutrition 0.000 description 1
- KYQZWONCHDNPDP-QNDFHXLGSA-N daidzein 7-O-beta-D-glucoside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CC=C2C(=O)C(C=3C=CC(O)=CC=3)=COC2=C1 KYQZWONCHDNPDP-QNDFHXLGSA-N 0.000 description 1
- 206010061428 decreased appetite Diseases 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 238000003795 desorption Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 235000013325 dietary fiber Nutrition 0.000 description 1
- 235000015872 dietary supplement Nutrition 0.000 description 1
- 235000019621 digestibility Nutrition 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 230000008034 disappearance Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 206010013395 disorientation Diseases 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 206010013781 dry mouth Diseases 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 210000005069 ears Anatomy 0.000 description 1
- 101150010415 eat-5 gene Proteins 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 238000004945 emulsification Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007071 enzymatic hydrolysis Effects 0.000 description 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 230000004438 eyesight Effects 0.000 description 1
- 239000012065 filter cake Substances 0.000 description 1
- 150000002213 flavones Chemical class 0.000 description 1
- 230000037406 food intake Effects 0.000 description 1
- 239000008369 fruit flavor Substances 0.000 description 1
- 235000012055 fruits and vegetables Nutrition 0.000 description 1
- 235000013376 functional food Nutrition 0.000 description 1
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 235000013922 glutamic acid Nutrition 0.000 description 1
- 239000004220 glutamic acid Substances 0.000 description 1
- 229930182470 glycoside Natural products 0.000 description 1
- 239000000665 guar gum Substances 0.000 description 1
- 235000010417 guar gum Nutrition 0.000 description 1
- 229960002154 guar gum Drugs 0.000 description 1
- 229920000591 gum Polymers 0.000 description 1
- 238000000265 homogenisation Methods 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229930195733 hydrocarbon Natural products 0.000 description 1
- 239000000852 hydrogen donor Substances 0.000 description 1
- 201000001421 hyperglycemia Diseases 0.000 description 1
- 230000005934 immune activation Effects 0.000 description 1
- 210000002865 immune cell Anatomy 0.000 description 1
- 230000003832 immune regulation Effects 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 208000016290 incoordination Diseases 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 201000002313 intestinal cancer Diseases 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 230000035987 intoxication Effects 0.000 description 1
- 231100000566 intoxication Toxicity 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 125000003690 ionone group Chemical group 0.000 description 1
- 230000007794 irritation Effects 0.000 description 1
- GOMNOOKGLZYEJT-UHFFFAOYSA-N isoflavone Chemical compound C=1OC2=CC=CC=C2C(=O)C=1C1=CC=CC=C1 GOMNOOKGLZYEJT-UHFFFAOYSA-N 0.000 description 1
- CJWQYWQDLBZGPD-UHFFFAOYSA-N isoflavone Natural products C1=C(OC)C(OC)=CC(OC)=C1C1=COC2=C(C=CC(C)(C)O3)C3=C(OC)C=C2C1=O CJWQYWQDLBZGPD-UHFFFAOYSA-N 0.000 description 1
- 235000008696 isoflavones Nutrition 0.000 description 1
- 230000002147 killing effect Effects 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 210000002429 large intestine Anatomy 0.000 description 1
- 235000005772 leucine Nutrition 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 210000005229 liver cell Anatomy 0.000 description 1
- 231100000849 liver cell damage Toxicity 0.000 description 1
- 210000005228 liver tissue Anatomy 0.000 description 1
- 239000000711 locust bean gum Substances 0.000 description 1
- 235000010420 locust bean gum Nutrition 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 210000002189 macula lutea Anatomy 0.000 description 1
- 235000019359 magnesium stearate Nutrition 0.000 description 1
- 229940035034 maltodextrin Drugs 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000004089 microcirculation Effects 0.000 description 1
- 230000027939 micturition Effects 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000004877 mucosa Anatomy 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 230000002107 myocardial effect Effects 0.000 description 1
- 210000004165 myocardium Anatomy 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 230000036542 oxidative stress Effects 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 239000000546 pharmaceutical excipient Substances 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 235000013930 proline Nutrition 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 239000008213 purified water Substances 0.000 description 1
- 230000000171 quenching effect Effects 0.000 description 1
- 239000001397 quillaja saponaria molina bark Substances 0.000 description 1
- 239000002516 radical scavenger Substances 0.000 description 1
- 239000001054 red pigment Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 210000001927 retinal artery Anatomy 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 229930182490 saponin Natural products 0.000 description 1
- 150000007949 saponins Chemical class 0.000 description 1
- 230000035807 sensation Effects 0.000 description 1
- 235000019615 sensations Nutrition 0.000 description 1
- 210000000813 small intestine Anatomy 0.000 description 1
- 235000014347 soups Nutrition 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 239000004834 spray adhesive Substances 0.000 description 1
- 238000013112 stability test Methods 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 208000011580 syndromic disease Diseases 0.000 description 1
- 238000009210 therapy by ultrasound Methods 0.000 description 1
- 231100000886 tinnitus Toxicity 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 230000002485 urinary effect Effects 0.000 description 1
- 235000019165 vitamin E Nutrition 0.000 description 1
- 229940046009 vitamin E Drugs 0.000 description 1
- 239000011709 vitamin E Substances 0.000 description 1
- 230000002618 waking effect Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 238000009736 wetting Methods 0.000 description 1
- 150000003735 xanthophylls Chemical class 0.000 description 1
- 235000008210 xanthophylls Nutrition 0.000 description 1
- OENHQHLEOONYIE-JLTXGRSLSA-N β-Carotene Chemical compound CC=1CCCC(C)(C)C=1\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C OENHQHLEOONYIE-JLTXGRSLSA-N 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
- A23G3/00—Sweetmeats; Confectionery; Marzipan; Coated or filled products
- A23G3/34—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof
- A23G3/36—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds
- A23G3/42—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds characterised by the carbohydrates used, e.g. polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
- A23G3/00—Sweetmeats; Confectionery; Marzipan; Coated or filled products
- A23G3/34—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof
- A23G3/36—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds
- A23G3/44—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds containing peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
- A23G3/00—Sweetmeats; Confectionery; Marzipan; Coated or filled products
- A23G3/34—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof
- A23G3/36—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds
- A23G3/48—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds containing plants or parts thereof, e.g. fruits, seeds, extracts
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/02—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation containing fruit or vegetable juices
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/385—Concentrates of non-alcoholic beverages
- A23L2/39—Dry compositions
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/42—Preservation of non-alcoholic beverages
- A23L2/44—Preservation of non-alcoholic beverages by adding preservatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L21/00—Marmalades, jams, jellies or the like; Products from apiculture; Preparation or treatment thereof
- A23L21/10—Marmalades; Jams; Jellies; Other similar fruit or vegetable compositions; Simulated fruit products
- A23L21/15—Marmalades; Jams; Jellies; Other similar fruit or vegetable compositions; Simulated fruit products derived from fruit or vegetable juices
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/3526—Organic compounds containing nitrogen
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/20—Reducing nutritive value; Dietetic products with reduced nutritive value
- A23L33/21—Addition of substantially indigestible substances, e.g. dietary fibres
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Polymers & Plastics (AREA)
- Food Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Mycology (AREA)
- Inorganic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention provides a composition for relieving alcoholism and protecting liver, a preparation method thereof and a product containing the composition. The composition of the present invention comprises puerariae radix extract, hovenia dulcis thunb extract, corn oligopeptide, turmeric extract and carotenoid. The product prepared by the components has good taste, good appearance, good solubility and stable efficacy through the synergistic effect of the components, and has the effects of relieving alcoholism, refreshing mind, protecting liver, relieving discomfort symptom of a drunk body and the like.
Description
Technical Field
The invention belongs to the technical field of functional foods, and relates to a composition for relieving alcoholism and protecting liver, a preparation method thereof and a product containing the composition.
Background
Alcohol and acetaldehyde, acetic acid, oxygen free radical and the like generated in the metabolic process of alcohol can cause damage to digestion, respiration, nerves, cardiovascular and cerebrovascular systems and urinary systems, and various diseases are caused. Therefore, the development of a natural, effective and convenient-to-drink anti-alcohol product without side effect is popular among the market and consumers.
Generally, the anti-hangover means that after a human body ingests a large amount of alcohol, the alcohol concentration can be decomposed, and symptoms such as dizziness, vomiting and the like caused by overhigh alcohol concentration can be relieved. At present, the products for preventing drunkenness and relieving alcoholism on the market are various in types, wherein most of components of the anti-alcoholism medicine only contain components such as an excitant, vitamins, amino acid and the like, and the effects of soothing, sobering, relieving headache and the like are provided, but the actual effect of relieving alcoholism is not achieved or is not obvious. Secondly, some anti-alcohol products in the market promote the decomposition speed of ethanol in the liver by adding ethanol dehydrogenase and acetaldehyde dehydrogenase which are biosynthesized, and the anti-alcohol products have higher cost and lower safety. In addition, some Chinese herbal medicine preparations are available on the market, and part of medicinal and edible Chinese herbal medicine raw materials are adopted, but the researches on raw material selection, compatibility mode, efficacy mechanism and efficacy component stability are all insufficient. Sang shu Tang is a classic traditional Chinese medicine, and was first recorded in the book of Tang Dynasty' Yuyuan Fang. Firstly, Tang Xuan Liangjiu good drink, which is drunk continuously for several days without fatigue, is recommended by all people, Xuan Shen Shu soup, which is recorded as: this is very surprising and is not good for drinking ears. The sangshu decoction is prepared from medicinal and edible components such as mulberry fruit, fructus alpiniae oxyphyllae and the like.
With the continuous improvement of the acceptance degree of Chinese herbal medicine health care functions in China in the international market, the Chinese herbal medicine preparation for relieving alcoholism and protecting liver needs to be deeply researched and developed, and a product which is safe and healthy, good in taste, obvious in effect and stable is designed.
Disclosure of Invention
Problems to be solved by the invention
In order to solve the problems in the prior art, the invention provides a composition for relieving alcoholism and protecting liver, and the prepared product has good taste, good appearance and good solubility, has the effects of relieving alcoholism, refreshing mind, protecting liver, relieving discomfort symptoms of drunk bodies and the like, and is relatively wide in applicable population.
The invention also provides a preparation method of the composition for learning and protecting liver, and the composition with stable content of functional components can be obtained in a large-scale industrial production process by adopting the method, and can be flexibly processed into products with different dosage forms according to requirements.
In addition, the invention also provides a product based on the anti-alcoholism and liver-protecting composition, and the product comprises a beverage.
Means for solving the problems
The invention provides the following technical scheme:
[1] a composition for relieving alcoholism and protecting liver comprises a kudzu root extract, a hovenia dulcis thunb extract, corn oligopeptide, a turmeric extract and carotenoid.
[2] The composition according to the item [1], wherein the content of puerarin in the composition accounts for 0.08-0.15 wt% of the total mass of the composition.
[3] The composition according to [1] or [2], wherein the composition does not include alcohol dehydrogenase and acetaldehyde dehydrogenase.
[4] The composition according to any one of [1] to [3], wherein,
the composition further comprises 15-100 parts by weight of hovenia dulcis thunb extract, 15-100 parts by weight of corn oligopeptide, 0.2-10 parts by weight of turmeric extract and no more than 0.6 part by weight of carotenoid, relative to 100 parts by weight of kudzu root extract.
[5] The composition according to any one of [1] to [4], wherein the carotenoid is selected from lutein.
[6] A preparation method of the composition for relieving alcoholism and protecting liver as described in any one of [1] to [5], wherein the preparation method comprises the steps of preparing kudzu root powder and raisin tree seed powder respectively, and then mixing the prepared kudzu root powder, raisin tree seed powder, corn oligopeptide powder, turmeric powder and carotenoid microcapsule powder in a mixer to obtain the composition.
[7] The preparation method according to [6], wherein the method for preparing the kudzu root powder or the hovenia dulcis thunb powder comprises the following steps: pretreatment, decoction and extraction, centrifugal separation, low-temperature concentration and spray drying.
[8] The production method according to [7], wherein the centrifugation step comprises centrifuging the extract by a butterfly centrifuge.
[9] The preparation method according to any one of [6] to [8], wherein the blending step comprises the following components in parts by weight:
10-30 parts of kudzu root powder
2-10 parts of semen hoveniae powder
2-10 parts of corn oligopeptide powder
0.1-0.5 part of turmeric powder
0.01-0.06 part of carotenoid micro-capsule powder.
[10] The production method according to any one of [6] to [9], wherein the production method further comprises a step of quality control of the kudzu root powder, the raisin tree seed powder and a step of quality control of the composition.
[11] The preparation method according to [10], wherein the step of quality control of the kudzu root powder comprises measuring the puerarin content in the kudzu root powder by liquid chromatography.
[12] The production method according to [10] or [11], wherein the step of quality control of hovenia dulcis thunb powder comprises measuring the content of total flavonoids in hovenia dulcis thunb powder by an ultraviolet absorbance method and measuring the content of dihydromyricetin in hovenia dulcis thunb powder by a liquid chromatography.
[13] The production method according to any one of [10] to [12], wherein the step of quality-controlling the composition comprises measuring a puerarin content in the composition by liquid chromatography.
[14] A product for relieving alcoholism and protecting liver, which comprises the composition for relieving alcoholism and protecting liver of any one of the items [1] to [3] and/or the composition prepared by the preparation method of any one of the items [6] to [13 ].
[15] The product for relieving alcoholism and protecting liver according to [14], wherein the product further comprises at least one prebiotic and/or at least one juice powder.
[16] The product for relieving alcoholism and protecting liver as recited in claim 15, wherein the prebiotics comprise one or more of fructo-oligosaccharide, galacto-oligosaccharide, xylo-oligosaccharide, malto-oligosaccharide, isomalto-oligosaccharide, soybean oligosaccharide and inulin.
[17] The product for relieving alcoholism and protecting liver according to [15], wherein the juice powder comprises one or more of mango juice powder, pomegranate juice powder, passion fruit juice powder, grape juice powder and cucumber juice powder.
[18] The product for relieving alcoholism and protecting liver as described in any one of [15] to [17], wherein the product further comprises natural food preservative such as nisin.
Effect of the inventionFruit
The composition for relieving alcoholism and protecting liver provided by the invention is added with various natural medicinal and edible extracts such as kudzuvine root, hovenia dulcis thunb and the like, and does not contain any hormone and harmful components of human body. The product has synergistic effect of different components, has the effects of relieving alcoholism, refreshing mind, protecting liver, reducing or inhibiting liver cell damage, resisting lipid peroxidation and relieving various body discomfort symptoms after drinking on the premise of ensuring safety and health, good taste, good appearance, good solubility and stable quality, and is suitable for relatively wide crowds. The kudzu root and corn oligopeptide in the composition form a layer of film insoluble in alcohol on gastrointestinal mucosa together to block the absorption of human body to alcohol, and the flavone, protein, polyphenol and/or prebiotics in the composition can synergistically reduce the absorption of gastrointestinal tract to alcohol, accelerate the metabolism of alcohol and acetaldehyde in liver, effectively relieve oxidative stress and immune activation reaction induced in the metabolic process, and achieve the purpose of strongly decomposing alcohol.
According to the preparation method of the composition for relieving alcoholism and protecting liver, the kudzuvine root and the hovenia dulcis thunb are independently extracted, centrifuged, concentrated and sprayed to obtain single spray-dried powder, the spray-dried powder is good in solubility and stable in content of functional components, further the finished product is good in solubility and obvious in effect, and the medication safety of traditional Chinese medicinal materials and the effectiveness and controllability of the quality of the finished product in a large-scale industrial production process can be guaranteed through a specific quality control method. The composition of the invention can be flexibly processed into products with different dosage forms according to requirements, including but not limited to tablets, capsules and granules; the product can be processed into different products on the basis of different dosage forms, including but not limited to liquid beverage, solid beverage, jelly, capsule and candy. Besides the composition disclosed by the invention, prebiotics can be added into the product, and can be matched with the composition disclosed by the invention to effectively improve the micro-ecology of the gastrointestinal tract of a body and promote the health of the gastrointestinal tract, so that the stimulation of alcohol to the gastrointestinal tract is reduced; the fruit juice powder is added to adjust the pH value of the whole system, ensure the stability of the turmeric, lutein and nisin, and can be used as a component for adjusting the flavor of the product; the high-efficiency and nontoxic natural food preservative nisin is added, can be degraded by chymotrypsin in a human body, does not remain in the human body, and has high stability and safety. The product of the invention has good taste, low calorie and good efficacy, and can be directly drunk and carried easily. The above description does not disclose all embodiments of the present invention and all advantages of the present invention.
Drawings
FIG. 1 shows the effect of consumption of 5 materials on the concentration of ethanol in human serum after drinking.
FIG. 2 shows the effect of consumption of example 3 of the present invention on the concentration of ethanol in human serum before and after drinking.
FIG. 3 shows the effect of consumption of example 3 of the present invention on alcohol-related parameters in mice before and after drinking alcohol. (FIG. 3A: the effect of example 3 before and after drinking on the concentration of ethanol in the serum of mice; FIG. 3B: the effect of example 3 before and after drinking on the gastric ADH activity of mice; FIG. 3C: the effect of example 3 before and after drinking on the ADH activity in the liver of mice A, B, C, indicating that there is a significant difference (p < 0.05) between the different experimental groups.)
FIG. 4 shows the effect of consumption of example 3 of the present invention on the relative transaminase in mice before drinking alcohol. (FIG. 4A: the effect of example 3 on ALT activity in mouse serum before drinking alcohol; FIG. 4B: the effect of example 3 on AST activity in mouse serum before drinking alcohol A, B, and c, which indicate that there is a significant difference (p < 0.05) between different experimental groups.)
Detailed Description
In the following detailed description, numerous specific details are set forth in order to provide a better understanding of the invention. It will be understood by those skilled in the art that the present invention may be practiced without some of these specific details. In other instances, methods, means, devices and steps which are well known to those skilled in the art have not been described in detail so as not to obscure the invention.
Unless defined otherwise, technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.
In the present specification, the numerical range represented by "numerical value a to numerical value B" means a range including the end point numerical value A, B.
In the present specification, the meaning of "may" includes both the meaning of performing a certain process and the meaning of not performing a certain process.
It should be understood that, as used in the specification and claims of this application, the singular forms "a," "an," and "the" include plural referents unless the context clearly dictates otherwise.
In the present specification, reference to "one or some particular/preferred embodiments", "another or some other particular/preferred embodiments", "one or another embodiment", or the like, means that a particular element (e.g., feature, structure, property, and/or characteristic) described in connection with the embodiment is included in at least one embodiment described herein, and may or may not be present in other embodiments. In addition, it is to be understood that the described elements may be combined in any suitable manner in the various embodiments.
The terms "comprises" and "comprising," and any variations thereof, in the description and claims of this invention and the above-described drawings are intended to cover non-exclusive inclusions. For example, a process, method, or system, article, or apparatus that comprises a list of steps or elements is not limited to only those steps or elements listed, but may alternatively include other steps or elements not listed, or inherent to such process, method, article, or apparatus.
The term "control", as used herein, unless otherwise indicated, refers to a standard substance used for identification, inspection, assay and calibration of the performance of assay instruments, generally approved by the national drug certification authority for examination, which standard should not be less than the quality standard for the product.
The term "test article" as used herein, unless otherwise specified, refers to an experimental sample used for detection or identification.
As used herein, unless otherwise specified, the term "precisely weigh" means that the weight is weighed to the nearest thousandth of the weight taken, the term "weigh" means that the weight is weighed to the nearest hundredth of the weight taken, the term "precisely measure" means that the volume is measured to the nearest thousandth of the volume taken, and the term "precisely aspirate" means that the sample is accurately measured by the microsyringe.
As used herein, unless otherwise indicated, the term "secondary filtrate" refers to the filtrate collected on filtration after discarding the primary filtrate. Compared with the primary filtrate, the secondary filtrate is closer to the real concentration of the sample, because the filtering medium (such as a filter membrane, filter paper and the like) can possibly adsorb the solute, the concentration of the sample in the primary filtrate is lower; in addition, the subsequent filtrate is cleaner, and because the solute adsorbed by the filter medium can form a filter cake, the filter pore size is reduced, and therefore, more tiny particles can be trapped.
As used herein, unless otherwise indicated, the term "filler" refers to a substance used as a stationary phase in chromatography, the term "stationary phase" refers to a phase that is immobilized in position in chromatography and exerts an adsorptive effect on a sample, and the term "mobile phase" refers to a phase that is not immobilized in position in chromatography and can freely flow and exert a desorption effect on a sample.
< composition for relieving alcoholism and protecting liver >
The invention provides a composition for relieving alcoholism and protecting liver. The composition of the present invention comprises puerariae radix extract, hovenia dulcis thunb extract, corn oligopeptide, turmeric extract and carotenoid. In some embodiments of the present invention, the composition of the present invention does not include biosynthetic alcohol dehydrogenase and acetaldehyde dehydrogenase, which reduces the cost and improves the safety, and although the composition of the present invention and the product prepared therefrom do not include the enzymes, the composition of the present invention can act on alcohol dehydrogenase and acetaldehyde dehydrogenase, so that the prepared product has the effects of alleviating hangover and protecting the liver. In other embodiments of the present invention, the composition of the present invention consists essentially of puerariae radix extract, hovenia dulcis thunb extract, corn oligopeptide, turmeric extract and carotenoid.
Unless otherwise stated, the term "kudzu root" in the present invention refers to the root of Pueraria lobata (Willd.) Ohwi, a perennial deciduous vine of the family Leguminosae, which is distributed in the north and south of China. Radix Puerariae is cool in nature, sweet and pungent in flavor, and has effects of relieving exterior syndrome, expelling pathogenic factors from muscles, invigorating yang, promoting eruption, relieving fever, and promoting fluid production. Modern medicine shows that the kudzuvine root can improve the blood circulation quality of heart and cerebral vessels, reduce the content of blood sugar, reduce the oxygen consumption of heart muscles, prevent hypertension and play a certain role in tinnitus. Radix Puerariae contains various flavonoids such as daidzin, daidzein, puerarin, etc. Wherein, the radix Puerariae total isoflavone has effects of improving neck pain, improving brain circulation, relieving angina pectoris, lowering blood pressure, reducing blood sugar, treating retinal artery obstruction, and enhancing immunity. The puerarin is a representative compound in the root of kudzu vine, is an isoflavone carbon glycoside compound, has certain functions of preventing drunkenness, relieving alcoholism, protecting liver, protecting health and the like, can resist the blood sugar increasing effect of adrenalin, has a certain blood sugar reducing effect, and has the functions of expanding blood vessels, improving blood circulation, reducing myocardial oxygen consumption, increasing coronary flow, regulating blood microcirculation and the like.
In some embodiments of the present invention, the pueraria extract includes pueraria powder, and the preparation method of the pueraria powder includes:
A) pretreatment: cleaning radix Puerariae, and drying;
B) decocting and extracting: adding the dried radix puerariae into an extraction tank, decocting twice with water with the volume being 6-8 times that of the material, 1-2 hours each time, filtering, and combining the extracting solutions for later use; in one embodiment of the invention, the filtration is performed using a 200 mesh screen;
C) centrifugal separation: carrying out centrifugal separation on the combined extracting solution to obtain a clarified solution; in a preferred embodiment, the centrifugal separation is carried out by a disk centrifuge, so that the dissolubility of the extract can be better ensured, and the peculiar smell substances can be removed;
D) low-temperature concentration: concentrating the clear solution at a low temperature, keeping the concentration temperature at 60-70 ℃, and then filtering the concentrated solution to obtain a clear concentrated solution; in one embodiment of the invention, a negative pressure concentrating tank is used for low temperature concentration; in another embodiment of the present invention, the concentrate is filtered through a 200 mesh screen when it is concentrated to a relative density of 1.11 to 1.13(65 ℃. + -. 5 ℃);
E) spray drying: heating the clear concentrated solution, stirring, filtering, spray drying to obtain dry powder, and sieving with 80 mesh sieve to obtain radix Puerariae powder.
The content of puerarin in the kudzu root powder is determined by high performance liquid chromatography, and in some embodiments of the invention, the content of puerarin in the kudzu root powder is not less than 6 wt%, further, the content of puerarin is not less than 7 wt%, and further, the content of puerarin is not less than 8 wt%.
The term "Hovenia dulcis thunb" in the present invention is, unless otherwise specified, mature seeds of Hovenia dulcis thunb, Hovenia acerba lindl, and Hovenia tricchart Chun et Tsiang, both belonging to the genus Hovenia of the family Rhamnaceae, and distributed in south China and provinces of western China. Semen Hoveniae is sweet in nature, mild in taste, and nontoxic, has effects of nourishing yin, promoting fluid production, moistening dryness, quenching thirst, cooling blood, clearing heat, promoting urination, and relieving alcoholism, and can be used for treating alcoholic intoxication, dysphoria with smothery sensation, thirst, emesis, and constipation. The main chemical components of the hovenia dulcis thunb comprise saponin, flavone, alkaloid and the like, and also contain a large amount of glucose, organic acid, peroxidase and the like, and under a certain condition, the hovenia dulcis thunb can expand the blood volume of a human body, remove excessive free radicals generated in the body after drinking wine, inhibit lipid peroxidation, further reduce the concentration of ethanol in blood, and further reduce the damage of the ethanol to liver tissues.
In some embodiments of the present invention, the hovenia dulcis thunb extract of the present invention comprises hovenia dulcis thunb powder, and in some embodiments of the present invention, the method for preparing hovenia dulcis thunb powder is as follows:
A) pretreatment: cleaning semen Hoveniae, and drying;
B) decocting and extracting: adding the dried radix puerariae into an extraction tank, decocting twice with water with the volume being 8-10 times that of the material, 1-2 hours each time, filtering, and combining the extracting solutions for later use; in one embodiment of the invention, the filtration is performed using a 200 mesh screen;
C) centrifugal separation: carrying out centrifugal separation on the combined extracting solution to obtain a clarified solution; in a preferred embodiment, the centrifugal separation is carried out by a disk centrifuge, so that the dissolubility of the extract can be better ensured, and the peculiar smell substances can be removed;
D) low-temperature concentration: concentrating the clear solution at a low temperature, keeping the concentration temperature at 60-70 ℃, and then filtering the concentrated solution to obtain a clear concentrated solution; in one embodiment of the invention, a negative pressure concentrating tank is used for low temperature concentration; in another embodiment of the present invention, the concentrate is filtered through a 200 mesh screen when it is concentrated to a relative density of 1.08 to 1.10(65 ℃. + -. 5 ℃);
E) spray drying: heating the clear concentrated solution, stirring uniformly, filtering, performing spray drying to obtain dry powder, and further sieving the dry powder with a 80-mesh sieve to obtain the hovenia dulcis thunb powder.
The total flavone in the hovenia dulcis thunb powder is determined by using an ultraviolet spectrophotometry, and in some embodiments of the invention, the content of the total flavone in the hovenia dulcis thunb powder is more than or equal to 6 wt%, further the content of the total flavone is more than or equal to 7 wt%, and further the content of the total flavone is more than or equal to 8 wt%. The content of dihydromyricetin in the hovenia dulcis thunb powder is determined by high performance liquid chromatography, and in some embodiments of the invention, the content of dihydromyricetin in the hovenia dulcis thunb powder is more than or equal to 1.5 wt%, further the content of dihydromyricetin is more than or equal to 2.0 wt%, and further the content of dihydromyricetin is more than or equal to 2.4 wt%.
Unless otherwise indicated, the term "corn oligopeptide" in the present invention is a mixture of small peptides (having an average molecular weight of less than 1000Da) produced by enzymatic hydrolysis (e.g., protease) using corn protein as the main raw material. As a new resource product, the corn oligopeptide is completely extracted from grains, and has the characteristics of natural source, no toxic or side effect, high activity and easy absorption by oral administration. Meanwhile, the corn oligopeptide is also rich in alanine, leucine, glutamic acid, proline and the like, and the special amino acid composition and connection mode of the corn oligopeptide endows the corn peptide with various biological activities, such as promotion of alcohol metabolism, protection of alcoholic chemical liver injury, fatigue resistance, tumor resistance, inhibition of angiotensin converting enzyme activity and the like. In some embodiments of the present invention, the adopted corn oligopeptide contains more than 95% of protein hydrolysate with molecular weight lower than 1000Da, i.e. the corn oligopeptide contains more than 95% of small peptides, so that the corn oligopeptide can be rapidly absorbed by the body, enters blood to exert physiological activity, and accelerates the sobering-up speed. Furthermore, the acid soluble protein content of the corn oligopeptide is more than 80%, which shows that the protein digestibility is high; furthermore, the content of free amino acid in the corn oligopeptide is more than 2 percent, and the free amino acid is an important taste component and a flavor precursor in fruits and vegetables and is used as a flavor component to contribute to forming the unique flavor of the product. In some embodiments of the invention, the corn oligopeptide (or corn oligopeptide powder) used may be commercially available, such as from Convergence, Inc., Guangzhou.
The term "turmeric" in the present invention is, unless otherwise specified, dried rhizome of Curcuma longa l. Curcumin is one of the main active ingredients of turmeric, has wide pharmacological actions such as liver protection, tumor resistance, mutation resistance, inflammation resistance, oxidation resistance and the like, has low toxicity, and is widely regarded. Curcumin has a greater antioxidant effect than vitamins, polyphenols and flavones, and is a natural antioxidant worthy of further tests and clinical studies. In addition, curcumin is not only a scavenger of free radicals, but also a hydrogen donor, so that the curcumin has dual activities of oxophilicity and oxidation resistance.
In some embodiments of the present invention, the turmeric extract of the present invention comprises turmeric powder, and in some embodiments of the present invention, the turmeric powder may be prepared by water decoction, centrifugation, concentration, drying, or the like, or may be obtained by a commercially available method, such as in some embodiments of the present invention, the turmeric powder is obtained from Hubei Noctt pharmaceutical Co.
Unless otherwise indicated, the term "carotenoid" in the present invention is a generic term for an important class of natural pigments, belonging to terpenoid compounds, which are commonly found in yellow, orange-red or red pigments in animals, higher plants, fungi, algae. Carotenoids can be divided into oxygenated carotenoids and non-oxygenated carotenoids, depending on their molecular composition. The oxygen-containing carotenoids are called xanthophylls (xanthylphenols), such as: carotenoid esters and carotenoid acids, etc.; non-oxygenated carotenoids are known as carotenes (carotenes) or carotenoid hydrocarbons. In the present invention the carotenoids may be selected from oxygen containing carotenoids and/or non-oxygen containing carotenoids such as lutein, beta-carotene and the like. In some preferred embodiments of the present invention, the mango juice powder is yellow in color, and orange lutein is added to the present composition, considering product function and color. Lutein, also known as lutein, contains ionone ring and multiple unsaturated double bonds, is also the main pigment constituting the macula lutea region of human eyes, and has certain effect on maintaining eye health. The lutein is nontoxic and safe, has strong physiological functions of resisting oxidation, improving eyesight and the like, and can be used as a food supplement to be added into food, beverage and the like. Multiple conjugated double bonds in the molecular structure of lutein give it the ability to quench singlet oxygen, and can react with free radicals to form harmless products or scavenge free radicals by breaking free radical chain reactions. In the aspect of immune regulation, lutein is used as a reactive oxygen free radical antagonist and can reduce the damage of reactive oxygen species (R0S) generated by the immune system to cell membranes, cell proteins, nucleic acids and the like of immune cells.
In some technical schemes, the carotenoid is from carotenoid microcapsule powder, and the stability of effective components is improved by microencapsulation technology, so that the carotenoid is not damaged in the digestive tract; in addition, the microcapsule powder is added with vitamin E, oligomeric maltose, etc., and has high safety and good dispersibility in cold water. In some embodiments of the present invention, the lutein microcapsule powder with a particle size of no more than 400 μm, further no more than 355 μm, is used for the present invention, which is more favorable for the efficient absorption in the small intestine, and can be obtained by a commercial method, for example, in some embodiments of the present invention, the lutein microcapsule powder is used from Dalian Denko corporation.
The puerarin is selected as an index of functional components and an index of stability evaluation of a finished product, and the content of the puerarin is measured by adopting a high performance liquid chromatography. In some technical schemes of the invention, the content of puerarin in the composition of the invention accounts for 0.08-0.15 wt% of the total mass of the composition, further the content is 0.09-0.12 wt%, further 0.09-0.10 wt%, and the finished product in the range has relatively more stable and excellent efficacy.
In some technical schemes of the invention, relative to 100 parts by weight of the kudzu root extract, the composition comprises 15-100 parts by weight of hovenia dulcis thunb extract, 15-100 parts by weight of corn oligopeptide, 0.2-10 parts by weight of turmeric extract and no more than 0.6 part by weight of carotenoid, and the proportion of each component in the range is favorable for better exerting the synergistic effect among the components and obtaining better effects of relieving alcoholism, protecting liver and resisting oxidation. Further, the composition comprises 20-80 parts by weight (further 30-50 parts by weight) of hovenia dulcis thunb extract, relative to 100 parts by weight of pueraria lobata extract; and/or, the composition comprises 20-80 parts by weight (further 30-50 parts by weight) of corn oligopeptide relative to 100 parts by weight of the kudzu root extract; and/or, the composition comprises 0.5-8 parts by weight (more preferably 0.8-5 parts by weight) of turmeric extract relative to 100 parts by weight of pueraria extract; and/or, the composition comprises 0.1-0.5 weight part of carotenoid relative to 100 weight parts of kudzu root extract.
< method for producing composition >
The invention provides a preparation method of the composition, which comprises the steps of respectively preparing kudzu root powder and hovenia dulcis thunb powder, and then blending the prepared kudzu root powder, hovenia dulcis thunb powder, corn oligopeptide powder, turmeric powder and carotenoid microcapsule powder in a mixer to obtain the composition. In a specific embodiment of the invention, the kudzu root powder, the hovenia dulcis thunb powder, the corn oligopeptide, the turmeric powder and the lutein microcapsule powder in the formula are mixed in a mixer for 20-40 minutes to obtain the composition with the functions of relieving alcoholism, protecting liver and resisting oxidation. In addition, as an alternative of the present invention, commercially available pueraria root powder and hovenia dulcis thunb powder can be selected, and then the pueraria root powder, hovenia dulcis thunb powder, corn oligopeptide powder, turmeric powder and carotenoid are put in a mixer for blending to obtain the composition.
In some technical schemes of the invention, the blending steps are as follows:
10-30 parts of kudzu root powder
2-10 parts of semen hoveniae powder
2-10 parts of corn oligopeptide powder
0.1-0.5 part of turmeric powder
0.01-0.06 part of carotenoid micro-capsule powder.
Further, the weight parts of the components are as follows:
10-20 parts of kudzu root powder
2-6 parts of semen hoveniae powder
2-6 parts of corn oligopeptide powder
0.2-0.4 part of turmeric powder
0.01-0.05 part of carotenoid micro-capsule powder.
In some technical schemes of the present invention, the preparation method of the present invention further comprises a step of performing quality control on the pueraria lobata powder and the hovenia dulcis thunb powder and a step of performing quality control on the composition, and further, the step of performing quality control on the pueraria lobata powder comprises a step of measuring the puerarin content in the pueraria lobata powder by using a liquid chromatography (including using HPLC or UPLC), for example, the puerarin content can be measured by using a high performance liquid chromatography 0512 according to the general rules of the four departments of the 2020 edition of chinese pharmacopoeia. Further, the step of quality control of the hovenia dulcis thunb powder comprises measuring the content of total flavonoids in the hovenia dulcis thunb powder by an ultraviolet absorbance method and measuring the content of dihydromyricetin in the hovenia dulcis thunb powder by a liquid chromatography (including HPLC or UPLC). In some embodiments of the present invention, in order to obtain more accurate determination results, the present inventors designed the following detection methods for the content determination of total flavonoids and dihydromyricetin in hovenia dulcis thunb powder:
and (3) measuring the content of the total flavonoids: preparation of a test solution: weighing sample powder, precisely weighing, adding appropriate amount of ethanol, ultrasonic treating, cooling, adding ethanol to desired scale, shaking, filtering, and collecting filtrate. Preparation of control solutions: weighing appropriate amount of dihydromyricetin (purity 98.0%) as reference, and dissolving in ethanol to obtain 0.1mg/mL solution. Preparation of a standard curve: precisely measuring the reference substance solutions of 0.1 mL, 0.5 mL, 1.0mL, 1.5 mL and 2.0mL, respectively placing in 25mL volumetric flasks, adding ethanol to the scales, measuring absorbance at 290nm wavelength, and drawing a standard curve with the absorbance as ordinate and the concentration as abscissa. The determination method comprises the following steps: precisely measuring 1.0mL of the test solution, placing the test solution in a 25mL volumetric flask, adding ethanol to a scale, measuring absorbance at 290nm wavelength, reading out the content of total flavonoids in the test solution from a standard curve, and calculating to obtain the total flavonoids.
And (3) measuring the content of dihydromyricetin: chromatographic conditions and system practicability test: octadecylsilane chemically bonded silica is used as a filling agent; taking methanol as a mobile phase A and a phosphoric acid solution as a mobile phase B, and carrying out gradient elution according to the specification in the following table; the detection wavelength was 290 nm. The number of theoretical plates is not less than 2000 calculated according to the peak of dihydromyricetin.
TABLE 1
Preparation of a test solution: weighing sample powder, precisely weighing, adding appropriate amount of ethanol, ultrasonic treating, cooling, adding ethanol to desired scale, shaking, filtering, and collecting filtrate. Further, the filtration may be performed by using a 0.45 μm membrane. Preparation of a reference solution: accurately weighing 5mg of dihydromyricetin reference substance, placing in a 100mL measuring flask, adding ethanol to scale, and shaking to obtain dihydromyricetin reference substance solution. The determination method comprises the following steps: precisely sucking 5 μ l of each of the reference solution and the sample solution, injecting into liquid chromatograph, and measuring.
According to the determination method, in some embodiments of the present invention, the content of puerarin in the prepared pueraria root powder is equal to or more than 6 wt%. In some embodiments of the invention, the total flavone content of the prepared hovenia dulcis thunb powder is required to be more than or equal to 4.5 wt%, and the content of dihydromyricetin is required to be more than or equal to 1.3 wt%. If the conditions are not met, the kudzu root powder or the raisin tree seed powder of the batch is not qualified.
The step of quality control of the composition in the present invention comprises measuring the puerarin content of the composition by liquid chromatography, including by HPLC or UPLC. The present inventors have conducted research and study on chromatographic conditions and a method for preparing a test solution. In a preferred embodiment of the present invention, the measuring method uses octadecylsilane chemically bonded silica as a filler; methanol-water (25:75) as the mobile phase; the detection wavelength was 250 nm. In one embodiment of the invention, considering the solubility and the complete extraction and the efficiency, the preparation of the sample solution comprises the steps of taking the composition, precisely weighing, placing the composition in a volumetric flask, dropwise adding ethanol to a near-scale line, vibrating while adding ethanol, sealing, ultrasonically extracting for 20-40 minutes, cooling, fixing the volume to the scale line with ethanol, shaking uniformly, centrifuging, filtering, and taking a continuous filtrate.
According to the above determination method, in some embodiments of the present invention, the puerarin content of the prepared composition should satisfy 0.08 wt% or more. If the above conditions are not satisfied, the batch of the composition is considered to be rejected.
According to the invention, the content of index components of the kudzu root powder, the hovenia dulcis thunb powder and the composition is determined, so that the quality control is more comprehensive and accurate, the high specificity and the high repeatability are achieved, and the safety and the controllability of subsequent products are ensured.
< products >
The invention further provides a product for relieving alcoholism and protecting liver, wherein the product contains the composition for relieving alcoholism and protecting liver and/or the composition prepared by the preparation method. The product of the invention has good taste, good appearance and stable quality, and also has the effects of relieving alcoholism, refreshing mind, protecting liver and relieving various body discomfort symptoms after drinking.
In some embodiments of the present invention, the product of the present invention may be directly the composition prepared by the aforementioned method of the present invention, and in other embodiments of the present invention, the product of the present invention may further comprise various food-acceptable excipients in addition to the composition of the present invention, and may be processed into various dosage forms, including but not limited to tablets, capsules, granules; the product can be processed into different products on the basis of different dosage forms, including but not limited to liquid beverage, solid beverage, jelly, capsule and candy. The food acceptable adjuvants include food gelatinizer, and the food adhesive includes one or more of karaya gum, Caesalpinia spinosa gum, locust bean gum, and guar gum.
In some embodiments of the invention, the product of the invention comprises at least one prebiotic (prebiotic) in order to further improve the efficacy of the product and reduce the irritation of alcohol to the gastrointestinal tract. The prebiotics of the invention include, but are not limited to, one or more of fructo-oligosaccharide, galacto-oligosaccharide, xylo-oligosaccharide, malto-oligosaccharide, isomalto-oligosaccharide, soybean oligosaccharide and inulin. Furthermore, the prebiotics are selected from fructo-oligosaccharides, the fructo-oligosaccharides are functional oligosaccharides with the polymerization degree of 2-9 and formed by connecting fructosyl groups through beta-2, 1-glycosidic bonds, have low energy and low sweetness, are water-soluble dietary fibers with excellent quality, are prebiotics capable of promoting the growth of bifidobacteria, have the sweetness of 30-60 percent of the mass fraction of sucrose, can directly enter the large intestine without being digested and absorbed by human bodies, are preferentially utilized by the bifidobacteria, are effective proliferation factors of the bifidobacteria, have the health-care effects of regulating constipation, regulating intestinal flora, promoting mineral absorption, regulating hypertension, hyperlipidemia and hyperglycemia, preventing and treating intestinal cancer and the like, and the inventors found that the fructo-oligosaccharides added into the product can generate a synergistic effect with the composition of the invention, and can further reduce the stimulation of alcohol to the gastrointestinal tract. The prebiotics are added in an amount of about 20% to about 40% by weight of the total composition of the invention.
In some embodiments of the present invention, the product of the present invention may further comprise a juice powder in order to further improve mouthfeel and improve system stability. The pH value of the whole system is adjusted by using the juice powder, the stability of the turmeric, the lutein and the optional added nisin is ensured, and the unique smell of the product can be formed with the corn oligopeptide in the composition, so that the mouthfeel of the product is further improved. The juice powder of the invention comprises one or more of mango juice powder, pomegranate juice powder, passion fruit juice powder, grape juice powder and green cucumber juice powder, and can endow products with different fruit flavors. In some preferred embodiments of the invention, the juice powder of the invention is selected from mango juice powder, taking into account the colour of the product.
In some embodiments of the invention, the product of the invention further comprises a minor amount of a natural food preservative such as nisin. The lactobacillin is active antibacterial protein or polypeptide generated in the metabolic process of lactic acid bacteria, can be degraded by chymotrypsin in a human body once entering the human body, does not remain in the human body, does not induce pathogenic drug-resistant strains in the human body, and does not generate cross resistance with other antibiotics.
In some technical schemes of the invention, the preparation method of the product of the invention comprises the step of uniformly mixing the composition of the invention with optionally added prebiotics, fruit juice powder, natural food preservatives and optionally added various food acceptable auxiliary materials according to formula dosage.
In some embodiments of the present invention, when producing a liquid beverage for alleviating hangover and protecting liver, 30 to 40 parts of the composition of the present invention, 30 to 60 parts of fruit juice powder, 20 to 40 parts of fructo-oligosaccharide, and optionally 0.04 to 0.06 part of nisin are added into a storage tank, the pH of the beverage is adjusted to 4.0 to 5.0 by using the fruit juice powder, 200 parts of hot water (purified water) is added into the storage tank, the mixture is uniformly stirred, the complete dissolution is ensured by emulsification and homogenization, and then the finished product is obtained by filling, high-temperature and high-pressure sterilization.
While ingestion of the product of the present invention is effective in alleviating the degree of intoxication, in some embodiments of the present invention, it is preferred that consumption of the product of the present invention prior to drinking is beneficial in enhancing the first pass effect of the stomach and reducing alcohol absorption. In addition, the product of the invention can play a certain role in protecting chronic alcoholic liver injury after being eaten for a long time.
The technical solution of the present invention will be further described with reference to specific examples. It will be readily understood by those skilled in the art that the specific experimental conditions and results thereof described in the following examples are illustrative of the present invention only and should not be, and should not be construed as, limiting the invention. Changes and substitutions in detail and form may be made without departing from the spirit and scope of the invention, but it is intended that such changes and substitutions fall within the scope of the invention. In addition, unless otherwise specified, instruments, materials, reagents and the like used in the examples can be obtained by conventional commercial means.
Examples
Example 1: composition and preparation of composition for dispelling effects of alcohol and protecting liver
(1) Preparing kudzu root powder:
cleaning radix Puerariae (purchased in national medicine system production base), removing impurities, drying, adding processed radix Puerariae into an extraction tank, boiling and extracting with water 8 times the volume of the material twice, each time for 1 hr, filtering with 200 mesh sieve, mixing filtrates to obtain extractive solution, and centrifuging the extractive solution with butterfly centrifuge to obtain clarified solution; placing the clarified solution in a negative pressure concentration tank, concentrating under reduced pressure at low temperature of 0.07Mpa at 65 + -5 deg.C until the relative density is 1.11-1.13(65 + -5 deg.C), and sieving with 200 mesh sieve to obtain clarified concentrated solution; heating, uniformly stirring and filtering the clarified concentrated solution, and then carrying out spray drying by a spray dryer, wherein the air inlet temperature is 175-: sieving at 80-90 deg.C with 80 mesh sieve to obtain radix Puerariae spray dried powder. Measuring the content of puerarin in the radix Puerariae powder by high performance liquid chromatography according to 0512 of the general rules of four departments in the 2020 edition of Chinese pharmacopoeia, wherein the content of puerarin in the radix Puerariae powder of the batch is 8.5 wt%.
(2) Preparing raisin tree seed powder:
cleaning semen Hoveniae (purchased in a production base of a traditional Chinese medicine system), removing impurities, drying, adding the treated semen Hoveniae into an extraction tank, boiling and extracting with water with the volume 10 times that of the material twice, each time for 1 hour, filtering with 200 meshes, mixing filtrates to obtain an extract, and performing centrifugal separation on the extract by using a butterfly centrifuge to obtain a clear solution; placing the clarified solution in a negative pressure concentration tank, concentrating under reduced pressure at low temperature of 0.07Mpa at 65 + -5 deg.C until the relative density is 1.08-1.10(65 + -5 deg.C), and sieving with 200 mesh sieve to obtain clarified concentrated solution; heating, uniformly stirring and filtering the clarified concentrated solution, and then performing spray drying by a spray dryer, wherein the air inlet temperature is 165-: sieving with 80 mesh sieve at 80-90 deg.C to obtain semen Hoveniae spray dried powder. The content of the functional components in the hovenia dulcis thunb is determined according to the following method: UV method for measuring total flavone content
Preparation of a test solution: weighing 0.2g of sample powder, precisely weighing, placing in a 100mL volumetric flask, pouring an appropriate amount of 60% ethanol, performing ultrasonic treatment for 30min, cooling, adding 60% ethanol to scale, shaking up, filtering, and collecting the subsequent filtrate.
Preparation of control solutions: weighing appropriate amount of dihydromyricetin (purity 98.0%) reference substance, and dissolving in 70% ethanol to obtain 0.1mg/mL solution.
Preparation of a standard curve: precisely measuring reference substance solutions 0.1 mL, 0.5 mL, 1.0mL, 1.5 mL and 2.0mL, respectively placing in 25mL volumetric flasks, adding 60% ethanol to the scale, measuring absorbance at 290nm wavelength, and drawing a standard curve with the absorbance as ordinate and the concentration as abscissa.
The determination method comprises the following steps: precisely measuring 1.0mL of sample solution, placing in a 25mL volumetric flask, adding 60% ethanol to scale, measuring absorbance at 290nm wavelength, and calculating according to standard curve to obtain total flavone content of 8.20% in semen Hoveniae powder
High performance liquid chromatography for measuring dihydromyricetin content
Chromatographic conditions and system practicability test: octadecylsilane chemically bonded silica is used as a filler (column length: 250 mm); gradient elution was carried out as specified in table 1 above using methanol as mobile phase a and 0.2% phosphoric acid solution as mobile phase B; the detection wavelength was 290 nm. The number of theoretical plates is not less than 2000 calculated according to the peak of dihydromyricetin.
Preparation of a test solution: filtering the solution obtained from the preparation of the test solution under the item of total flavone determination with 0.45 μm membrane, and collecting the filtrate.
Preparation of a reference solution: accurately weighing 5mg of dihydromyricetin reference substance, placing in a 100mL measuring flask, adding 60% ethanol to scale, and shaking to obtain dihydromyricetin reference substance solution.
The determination method comprises the following steps: precisely sucking 5 μ L of each of the reference solution and the sample solution, injecting into a liquid chromatograph, and measuring to obtain 2.5% dihydromyricetin content in the semen Hoveniae powder.
(3) Corn oligopeptides were purchased from guangzhou honest and truthful industries, ltd; the protein hydrolysate with molecular weight less than 1000 accounts for over 95% of the total mass, the content of acid soluble protein is more than 80%, and the content of free amino acid is more than 2%.
(4) Curcuma rhizome powder purchased from Hubei Nuokete pharmaceutical industry GmbH
(5) The lutein microcapsule powder is purchased from Dalian medicine Nobio, Co, and has a particle size of 95% passing through 40 meshes.
The kudzu vine root powder, the hovenia dulcis thunb powder, the corn oligopeptide, the turmeric powder and the lutein microcapsule powder with the following formula amount are mixed in a mixer for 30 minutes to obtain the composition with the functions of dispelling the effects of alcohol, protecting the liver and resisting oxidation.
The formula comprises the following components: 1000g of kudzuvine root powder, 330g of hovenia dulcis thunb powder, 350g of corn oligopeptide powder, 23g of turmeric powder and 5g of lutein microcapsule powder.
The puerarin content in the composition is determined by the following method:
chromatographic conditions and system applicability test: octadecylsilane chemically bonded silica is used as a filling agent; methanol-water (25:75) as the mobile phase; the detection wavelength was 250 nm.
Preparation of control solutions: precisely weighing appropriate amount of puerarin control, adding 30% ethanol solution to obtain solution containing 80 μ g of puerarin per 1mL, and shaking.
Preparation of a test solution: precisely weighing about 1.0g of the prepared composition, placing the composition into a 25mL volumetric flask, dropwise adding 30% ethanol close to a scale mark while adding, oscillating, sealing, ultrasonically extracting for 30 minutes, cooling, fixing the volume to the scale mark with 30% ethanol, shaking uniformly, centrifuging at 4000r for 10min, filtering, and taking a subsequent filtrate to obtain the composition.
The determination method comprises the following steps: precisely sucking 10 μ L of each of the reference solution and the sample solution, injecting into liquid chromatograph, and measuring to obtain the composition with puerarin content of 0.095%.
Accelerated stability tests are carried out under the conditions that the temperature is 40 +/-2 ℃ and the relative humidity is 75% +/-5%, the results are shown in table 2 after accelerated 6-month investigation, and the puerarin content in the composition is stable, so that the puerarin can be used as the evaluation index of the stability of finished products.
TABLE 2
| Time | T0(0 month) | T1(1 month) | T2(2 months) | T3(3 months) | T6(6 months) |
| Puerarin content | 0.095％ | 0.094％ | 0.094％ | 0.095％ | 0.096％ |
Example 2: preparation of solid beverage for dispelling effects of alcohol and protecting liver
168g of the composition prepared in example 1, 240g of pomegranate juice powder and 133g of galacto-oligosaccharide were put into a hopper and mixed for 20 min. The water is used as a spray adhesive, fluidized granulation is carried out by fluidized bed granulation equipment, intermittent sampling observation is carried out, corresponding adjustment is carried out according to the granule condition, and the water content is controlled to be less than or equal to 6.0 percent. And (4) carrying out whole-particle sieving on the dried mixed particles, and collecting particles with the particle size of 18-60 meshes.
Example 3: preparation of liquid beverage for dispelling effects of alcohol and protecting liver
400g of the composition prepared in example 1, 560g of mango juice powder, 320g of fructo-oligosaccharide and 0.5g of nisin are added into a storage tank. Adjusting pH of the beverage to 4.0-5.0 with mango juice powder. Adding 1.9kg of hot water into a material storage tank, uniformly stirring, emulsifying and homogenizing to ensure that the materials are completely dissolved, and then filling and sterilizing at high temperature and high pressure to obtain a finished product.
Example 4: preparation of jelly for dispelling effects of alcohol and protecting liver
Taking 400g of the composition prepared in example 1, 560g of mango juice powder, 300g of fructo-oligosaccharide, 0.5g of nisin and a proper amount of karaya gum, adding the mixture into a storage tank, adding a proper amount of hot water into the storage tank, uniformly stirring, emulsifying and homogenizing to ensure that the mixture is completely dissolved, and then carrying out hot filling, high-temperature sterilization and cooling to obtain a finished product.
Example 5: preparation of capsule for relieving alcoholism and protecting liver
560g of the composition prepared in example 1, 5g of maltodextrin DE 2015 g and magnesium stearate were mixed in a mixer for 40 minutes to give granules of 18-60 mesh, and then mixed with 5g of aerosil in a mixer for 5 minutes to give a uniform mixture. And (5) placing the totally mixed materials in a full-automatic capsule filling machine, and filling capsules.
Comparative example 1: composition and preparation of Complex A
Compound A was prepared as in example 1, except that lutein microcapsule powder was not added.
Comparative example 2: composition and preparation of Complex B
The lutein microcapsule powder in example 1 was changed to licorice powder of the same weight part, and the rest of the same procedure was carried out in example 1 to prepare compound B.
The licorice powder is prepared as follows: wetting liquorice slices; adding the processed Glycyrrhrizae radix into an extraction tank, decocting with 10 times of water for 1 hr twice, filtering with 200 mesh sieve, mixing filtrates to obtain extractive solution, and centrifuging the extractive solution with a butterfly centrifuge to obtain clarified solution; placing the clarified solution in a negative pressure concentration tank, concentrating under reduced pressure at low temperature of 0.07Mpa at 70 + -5 deg.C until the relative density is 1.08-1.10(65 + -5 deg.C), and sieving with 200 mesh sieve to obtain clarified concentrated solution; heating, uniformly stirring and filtering the clarified concentrated solution, and then performing spray drying by a spray dryer, wherein the air inlet temperature is 165-: sieving at 80-90 deg.C with 80 mesh sieve to obtain Glycyrrhrizae radix powder.
Test example 1: crowd eating trial test of composition for dispelling effects of alcohol and protecting liver
To evaluate the effect of the composition of the present invention in alleviating hangover, 120 volunteers were selected and divided into four groups: blank group (drinking, not eating any sobering material), post-drinking group 1 (drinking immediately after drinking 15 g), post-drinking group 2 (drinking immediately after drinking 14 g), post-drinking group 3 (drinking immediately after drinking 25 g), post-drinking group 4 (drinking immediately after drinking 2g), post-drinking group 5 (drinking immediately after drinking 2 g). Wherein, the kudzu root powder and the hovenia dulcis thunb powder are prepared by the corresponding method recorded in the embodiment 1. The drinking condition is that 300mL of 12-degree red wine is drunk, the breath alcohol content is tested 30min after drinking, and then a breath alcohol tester is adopted to collect data every 30min, and the test time is 2 h. The ratio of the Blood Alcohol Concentration (BAC) to the breath alcohol concentration (BrAC) is 2200, which is a standard value in continental china, and the conversion relationship between the two units is BAC ═ BrAC × 2200. The effect of eating 5 materials after drinking on the concentration of ethanol in human serum is shown in figure 1.
As can be seen from FIG. 1, the alcohol concentration in the breath of the drinker is significantly decreased with time. After drinkers eat 5 materials immediately after drinking, the concentration of ethanol in blood is obviously lower than that of uneaten people, the reduction speed of the concentration of the ethanol in serum of people eating the wine is relatively fastest, and the blood is respectively the comparative example 2, the comparative example 1, the kudzu root powder and the hovenia dulcis thunb powder, so that the decomposition of the alcohol in a human body can be accelerated by the single raw materials of the kudzu root powder and the hovenia dulcis thunb powder, but the compound effect is better. Meanwhile, as for the compound in the comparative example 2, the liquorice powder and the lutein are added, and analysis shows that both can play a synergistic effect capability in the aspect of relieving alcoholism, but the synergistic effect of the lutein is relatively better than that of the liquorice powder, and in addition, considering that the compound of the liquorice powder is poor in taste and heavy in aftertaste, the lutein is preferred.
Meanwhile, questionnaires of each volunteer about hangover symptoms are collected and counted. The questionnaire was classified into 5 grades (very effective fruit: 5 points; effective fruit: 4 points; effect generally: 3 points; effect is a little poor: 2 points; effect is a very poor: 1 point) for 7 items with a dizziness relieving effect, a dry mouth relieving effect, a fatigue relieving effect, a refreshing effect, a sleepiness relieving effect, a headache relieving effect, a vomiting relieving effect, and the like, and the effects thereof were evaluated. The results of the effects of 5 materials gavage after drinking on hangover symptoms in humans are shown in Table 3.
TABLE 3
Hangover response refers to the symptoms of sleepiness, dizziness, disorientation, and fine motor incoordination in the morning after the administration of alcohol, which may be related to the slow elimination of alcohol in the human body and delayed action. As shown by the scores in Table 3, the drinker can feel a series of hangover symptoms such as sleepiness, dizziness after waking up, headache, thirst, intolerance, physical fatigue, retching, inappetence, even restlessness and the like in the next day.
The hangover related correlation scores in the post-alcohol group were all increased compared to the blank group, with the average score between 3.64-3.72 for post-alcohol group 2, post-alcohol group 4 and post-alcohol group 5, indicating that these 3 groups improved the post-alcohol symptoms, but the effect was general; the average scores of the post-alcohol group 1 and the post-alcohol group 3 ranged from 3.92 to 4.11, indicating that these 2 groups had significant effects in relieving post-alcohol symptoms, with the post-alcohol group 1 being relatively more effective.
Test example 2: crowd eating trial test of liquid beverage for dispelling effects of alcohol and protecting liver
Selecting 90 volunteers, and dividing the volunteers into three groups: blank group (drinking wine, not drinking this product), before drinking group (drinking example 3 half an hour before drinking wine), after drinking group (drinking example 3 after drinking wine). The drinking condition is that 300mL of 12-degree red wine is drunk, the breath alcohol content is tested 30min after drinking, and then a breath alcohol tester is adopted to collect data every 30min, and the test time is 2 h. The ratio of the Blood Alcohol Concentration (BAC) to the breath alcohol concentration (BrAC) is 2200, which is a standard value in continental china, and the conversion relationship between the two units is BAC ═ BrAC × 2200. The effect of consumption of example 3 on the concentration of ethanol in human serum before and after drinking is shown in FIG. 2.
As can be seen from FIG. 2, the alcohol concentration in the breath of the drinker is significantly decreased with time. After drinking the wine, the alcohol concentration in blood is obviously lower than that of the people who do not eat the wine, and the alcohol content of the wine before drinking is obviously lower than that of the wine after 30 min. The descending trend analysis of the data of 0-120min is integrated, and the liquid beverage provided by the invention has a good effect on accelerating alcohol metabolism, wherein the effect is better when the liquid beverage is eaten 30min before drinking.
Test example 3: relieving effect on acute alcoholism mouse
SPF grade healthy C57BL/6 male mice, 30, 8 weeks old, were randomized into 3 groups of 10 mice each after one week of acclimatized feeding. The wine is a blank group (56-degree white wine), a before-drinking group (gastric lavage example 3 half an hour before drinking wine) and a after-drinking group (gastric lavage example 3 after drinking wine). The product is 40g for healthy adults, and the weight of the adult is calculated by 70kg, which is equivalent to 100mg for mice. After fasting for 12h, mice were bled from the fasting orbital venous plexus. The experimental groups were gavaged with 15g/kg (bw) of white wine, then sacrificed by cervical dislocation, and blood and tissue samples were collected. During the experiment, the feeding environment is strictly controlled in the environment of temperature (25 +/-2) DEG C, relative humidity (50 +/-5)%, illumination for 12h and night for 12h, and the mice freely eat and are filled with water. The methods used in the animal experiments all conform to the European Union animal guidelines (Directive 2010/63/EU).
1. And (3) determining a behavioral index: 10 mice were placed on a vertical metal net immediately after gavage of each group, and the time until the mice fell off the metal net was recorded. The mice were then placed on a flat plate in a reversed manner, and the disappearance time of the righting reflex was observed and recorded (i.e., the mice were placed in a reversed manner, and if the posture was kept in a back-down state for 30 seconds or more, the righting reflex was judged to have disappeared).
2. Determination of biochemical index
1) And (3) serum ethanol determination: blood samples of mice were collected and the concentration of ethanol in serum was determined by means of the kit.
2) Determination of alcohol dehydrogenase in stomach: taking out the stomach immediately after the sacrifice, taking out the stomach contents, washing the stomach contents with cold normal saline, preparing the stomach tissue homogenate, and measuring the ADH activity in the tissue.
3) Alcohol dehydrogenase assay in liver: the liver was taken out immediately after sacrifice, washed clean with cold physiological saline to prepare liver homogenate, and ADH activity in the tissue was measured.
3. Results of the experiment
Results of the effect of gavage before and after drinking example 3 on the behavior of mice intoxicated are shown in table 4.
TABLE 4
Note: a, b and c, which show that the difference between different experimental groups is significant (p < 0.05)
The results of the effect of example 3 on alcohol-related parameters in mice before and after drinking are shown in FIG. 3. FIG. 3A: the effect of consumption of example 3 on the concentration of ethanol in the serum of mice before and after drinking; FIG. 3B: effect of consumption of example 3 on gastric ADH activity in mice before and after drinking; FIG. 3C: effect of consumption of example 3 on ADH activity in mouse liver before and after drinking. a, b and c, which show that the difference between different experimental groups is significant (p < 0.05).
The content of ethanol in the serum of mice in the group before and after drinking is obviously less than that of mice in the blank group, and by combining the activities of ADH enzymes in the stomach and the liver, the intake of the product is speculated to be beneficial to improving the activities of ADH in the stomach and the liver of the mice, so that more alcohol is promoted to be metabolized in the stomach, the absorption of an organism to the ethanol is reduced, the metabolism speed of the liver to the absorbed alcohol is accelerated, and the drunkenness symptom is relieved. In addition, the alcohol content in the mice in the pre-alcoholic group was relatively lower, probably because pre-alcoholic consumption was more favorable to enhance the first-pass effect of the stomach and reduce alcohol absorption.
Test example 4: protective action on chronic alcoholic liver injury of mice
SPF grade healthy C57BL/6 male mice, 30, 8 weeks old, were randomized into 3 groups of 10 mice each after one week of acclimatized feeding. The test samples were respectively blank (gavage normal saline), model building (30 degree distilled spirit) and prevention (edible example 3+30 degree distilled spirit). The product is 40g for healthy adults, and the weight of the adult is calculated by 70kg, which is equivalent to 100mg for mice. Therefore, the mice are subjected to intragastric administration for 1 time every day, 100mg of intragastric administration is carried out every time, the intragastric administration is carried out for 15d continuously, 30-degree white spirit (15mL/kg) is given to the model building group and the prevention group after sample administration for 30min, the same amount of 0.9% physiological saline is given to the blank group, eyeballs are picked after 12h of anesthesia in a fasting state for blood sampling, the cervical vertebra dislocation method is used for killing, and blood samples are collected. The activity of glutamic-oxaloacetic transaminase and glutamic-pyruvic transaminase in serum is measured by a full-automatic serum biochemical analyzer. During the experiment, the feeding environment is strictly controlled in the environment of temperature (25 +/-2) DEG C, relative humidity (50 +/-5)%, illumination for 12h and night for 12h, and the mice freely eat and are filled with water. The methods used in the animal experiments all conform to the European Union animal guidelines (Directive 2010/63/EU).
The effect of consumption of example 3 on the relevant transaminases in mice before drinking alcohol is shown in figure 4. FIG. 4A: effect of consumption of example 3 on ALT activity in mouse serum before drinking; FIG. 4B: effect of consumption of example 3 on AST activity in mouse serum before drinking. a, b and c, which show that the difference between different experimental groups is significant (p < 0.05). Alanine Aminotransferase (ALT) and aspartate Aminotransferase (AST) are primarily present in the plasma of liver cells, and when the liver is damaged, the intracellular aminotransferase enters the blood, resulting in elevated levels of ALT and AsT in the blood. According to the test result, the ALT and AST activity in the serum of the mouse is obviously increased due to the chronic alcohol exposure, and the ALT and AST levels of the serum of the mouse can be effectively recovered after the product is taken, which shows that the product can play a certain role in protecting the chronic alcoholic liver injury after being eaten for a long time.
It should be understood by those skilled in the art that the above embodiments are only for illustrating the present invention and are not to be used as limitations of the present invention, and modifications, variations and changes of the above embodiments are within the scope of the present invention as long as they are within the spirit and scope of the present invention.
Claims (10)
1. The composition for relieving alcoholism and protecting liver is characterized by comprising a kudzu root extract, a hovenia dulcis thunb extract, corn oligopeptide, a turmeric extract and carotenoid.
2. The composition as claimed in claim 1, wherein the puerarin content in the composition is 0.08-0.15 wt% of the total mass of the composition.
3. The composition according to any one of claims 1 or 2,
the composition further comprises 15-100 parts by weight of hovenia dulcis thunb extract, 15-100 parts by weight of corn oligopeptide, 0.2-10 parts by weight of turmeric extract and no more than 0.6 part by weight of carotenoid, relative to 100 parts by weight of kudzu root extract, and further preferably, the carotenoid is selected from lutein.
4. A preparation method of the composition for relieving alcoholism and protecting liver as claimed in any one of claims 1-3, wherein the preparation method comprises preparing radix Puerariae powder and semen Hoveniae powder respectively, and then mixing the prepared radix Puerariae powder, semen Hoveniae powder, corn oligopeptide powder, Curcuma rhizome powder and carotenoid microcapsule powder in a mixer to obtain the composition.
5. The preparation method according to claim 4, wherein the method for preparing the kudzu root powder or the hovenia dulcis thunb powder comprises the following steps: pretreatment, decoction extraction, centrifugation, low-temperature concentration and spray drying, and further preferably, the centrifugation step comprises centrifuging the extract by a butterfly centrifuge.
6. The preparation method according to claim 4 or 5, wherein the blending step comprises the following components in parts by weight:
10-30 parts of kudzu root powder
2-10 parts of semen hoveniae powder
2-10 parts of corn oligopeptide powder
0.1-0.5 part of turmeric powder
0.01-0.06 part of carotenoid micro-capsule powder.
7. The preparation method according to any one of claims 4 to 6, further comprising a step of quality control of the kudzu root powder and the raisin tree seed powder and a step of quality control of the composition, further preferably the step of quality control of the kudzu root powder comprises determination of puerarin content in the kudzu root powder by liquid chromatography, further preferably the step of quality control of the raisin tree seed powder comprises determination of total flavone content in the raisin tree seed powder by ultraviolet absorbance method and determination of dihydromyricetin content in the raisin tree seed powder by liquid chromatography, further preferably the step of quality control of the composition comprises determination of puerarin content in the composition by liquid chromatography.
8. A product for relieving or neutralizing the effect of alcohol and protecting liver, which is characterized by comprising the composition for relieving or neutralizing the effect of alcohol and protecting liver as claimed in any one of claims 1 to 3 and/or the composition prepared by the preparation method as claimed in any one of claims 4 to 7.
9. The anti-hangover and hepatoprotective product of claim 8, further comprising at least one prebiotic and/or at least one juice powder; further preferably, the prebiotics comprise one or more of fructo-oligosaccharide, galacto-oligosaccharide, xylo-oligosaccharide, malto-oligosaccharide, isomalto-oligosaccharide, soy oligosaccharide and inulin; still further preferably, the juice powder comprises one or more of mango juice powder, pomegranate juice powder, passion fruit juice powder, grape juice powder, and cucumber juice powder.
10. The product for alleviating hangover and protecting liver as claimed in claim 9, further comprising a natural food preservative such as nisin.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011520581.3A CN112690450A (en) | 2020-12-21 | 2020-12-21 | Composition for relieving alcoholism and protecting liver, preparation method thereof and product containing composition |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011520581.3A CN112690450A (en) | 2020-12-21 | 2020-12-21 | Composition for relieving alcoholism and protecting liver, preparation method thereof and product containing composition |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112690450A true CN112690450A (en) | 2021-04-23 |
Family
ID=75509458
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011520581.3A Pending CN112690450A (en) | 2020-12-21 | 2020-12-21 | Composition for relieving alcoholism and protecting liver, preparation method thereof and product containing composition |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112690450A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114128813A (en) * | 2021-12-09 | 2022-03-04 | 天津创源生物技术有限公司 | Beverage for relieving alcoholism and protecting liver and promoting vitality of liver and preparation method thereof |
| CN115299614A (en) * | 2022-08-05 | 2022-11-08 | 仁和全域(上海)大健康研究院有限公司 | Hangover alleviating composition, hangover alleviating jelly and preparation method and application of hangover alleviating jelly |
| IT202200008018A1 (en) * | 2022-04-22 | 2023-10-22 | Calliero S P A | PROCESS FOR THE PULVERISATION AND STABILIZATION OF ESSENTIAL OILS INCLUDING FURANODIENES |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107853532A (en) * | 2017-12-14 | 2018-03-30 | 江西颐迪科技有限公司 | A kind of Antialcoholic liver-protecting protect liver solid beverage and preparation method thereof |
| CN109007808A (en) * | 2018-08-14 | 2018-12-18 | 宝健（北京）生物技术有限公司 | A kind of relieving alcoholism and protecting liver composition and the relieving alcoholism and protecting liver preparation comprising it |
| CN109170843A (en) * | 2018-07-05 | 2019-01-11 | 绵阳市润土农业科技开发有限公司 | Internal ferment and preparation method thereof for alleviating alcoholism |
| CN109432386A (en) * | 2018-11-08 | 2019-03-08 | 武汉森澜生物科技有限公司 | A kind of corn peptide combinations of relieving alcoholism and protecting liver and preparation method thereof |
| CN110269166A (en) * | 2019-07-04 | 2019-09-24 | 北京九前健康科技有限公司 | A kind of preparation method of relieving alcoholism and protecting liver composition and drink and drink |
| CN111375049A (en) * | 2018-12-29 | 2020-07-07 | 辽宁上药好护士药业（集团）有限公司 | Composition for relieving alcoholism and protecting liver as well as preparation method and application thereof |
| CN111671026A (en) * | 2020-07-31 | 2020-09-18 | 广东粤微食用菌技术有限公司 | Turmeric composite alcohol-relieving and liver-protecting drink for relieving alcohol, dispelling effects of alcohol and protecting gastrointestinal tract and preparation method thereof |
-
2020
- 2020-12-21 CN CN202011520581.3A patent/CN112690450A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107853532A (en) * | 2017-12-14 | 2018-03-30 | 江西颐迪科技有限公司 | A kind of Antialcoholic liver-protecting protect liver solid beverage and preparation method thereof |
| CN109170843A (en) * | 2018-07-05 | 2019-01-11 | 绵阳市润土农业科技开发有限公司 | Internal ferment and preparation method thereof for alleviating alcoholism |
| CN109007808A (en) * | 2018-08-14 | 2018-12-18 | 宝健（北京）生物技术有限公司 | A kind of relieving alcoholism and protecting liver composition and the relieving alcoholism and protecting liver preparation comprising it |
| CN109432386A (en) * | 2018-11-08 | 2019-03-08 | 武汉森澜生物科技有限公司 | A kind of corn peptide combinations of relieving alcoholism and protecting liver and preparation method thereof |
| CN111375049A (en) * | 2018-12-29 | 2020-07-07 | 辽宁上药好护士药业（集团）有限公司 | Composition for relieving alcoholism and protecting liver as well as preparation method and application thereof |
| CN110269166A (en) * | 2019-07-04 | 2019-09-24 | 北京九前健康科技有限公司 | A kind of preparation method of relieving alcoholism and protecting liver composition and drink and drink |
| CN111671026A (en) * | 2020-07-31 | 2020-09-18 | 广东粤微食用菌技术有限公司 | Turmeric composite alcohol-relieving and liver-protecting drink for relieving alcohol, dispelling effects of alcohol and protecting gastrointestinal tract and preparation method thereof |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114128813A (en) * | 2021-12-09 | 2022-03-04 | 天津创源生物技术有限公司 | Beverage for relieving alcoholism and protecting liver and promoting vitality of liver and preparation method thereof |
| IT202200008018A1 (en) * | 2022-04-22 | 2023-10-22 | Calliero S P A | PROCESS FOR THE PULVERISATION AND STABILIZATION OF ESSENTIAL OILS INCLUDING FURANODIENES |
| CN115299614A (en) * | 2022-08-05 | 2022-11-08 | 仁和全域(上海)大健康研究院有限公司 | Hangover alleviating composition, hangover alleviating jelly and preparation method and application of hangover alleviating jelly |
| CN115299614B (en) * | 2022-08-05 | 2024-05-14 | 仁和全域(上海)大健康研究院有限公司 | Anti-alcohol composition, anti-alcohol jelly and preparation method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104432084B (en) | A kind of conditioning powder and its production and use | |
| KR102076459B1 (en) | Composition containing enzyme treatmented Tenebrio molitor for muscle mass enhancement and restorative rejuvenation of recovering patients or elderly patients | |
| CN102552462B (en) | Effervescent preparation as well as preparation method and application thereof | |
| CN102273639B (en) | Nidus collocaliae perfume (or spice) system product and preparation method thereof | |
| CN112690450A (en) | Composition for relieving alcoholism and protecting liver, preparation method thereof and product containing composition | |
| KR101433188B1 (en) | Composition For Relieving Alcohol-induced Hangover Comprising Starch, Amino Acids, Active Complex Vitamin And Oriental Herb Medicine materials AND, The Manufacturing Method Thereof | |
| CN104799146A (en) | Functional nutritional good composition for improving sub-health and preparation method thereof | |
| TWI500391B (en) | Composition for reliving and preventing hangover and beverage comprising composition | |
| CN106975069B (en) | Liver-protecting composition and preparation method thereof | |
| CN110447902B (en) | Composition with weight-losing effect | |
| CN104643250A (en) | Solid beverage capable of bidirectionally regulating body weight and improving subhealth | |
| CN110269224A (en) | A kind of elder jelly and preparation method thereof | |
| CN106135891A (en) | A kind of health food to alcoholic liver injury with defencive function | |
| KR20130003520A (en) | Composition for preventing or relieving alcohol-induced hangover comprising medicinal herbs | |
| CN102524781A (en) | Edible composition with alcoholism alleviation function and preparation method thereof | |
| CN107455625A (en) | A kind of nutrient for plants beverage and its preparation technology with antifatigue weight losing function | |
| KR101919839B1 (en) | Composition for hepatoprotective and ameliorating hangover containing cricket material enzyme-decomposed with Alcalase | |
| KR101930277B1 (en) | Composition for Preventing and Improving Hangover | |
| CN112618608A (en) | Composition with function of dispelling effects of alcohol and preparation method and application thereof | |
| KR101394358B1 (en) | Health composition relieving alcohol hangover | |
| CN105920198B (en) | Cell wall-broken micropowder with blood replenishing effect and preparation method thereof | |
| KR101228920B1 (en) | A composition comprising of a leaf extract of dendropanax morbifera for treating and preventing intestinal function disorder | |
| CN111357891A (en) | Plant beverage containing artichoke and ginseng and preparation method thereof | |
| CN108245531B (en) | Composition for improving gastrointestinal tract function and preventing and treating constipation | |
| KR101034986B1 (en) | Composition comprising the mixed herbal extract for relieving hangover |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |