KR101919839B1 - Composition for hepatoprotective and ameliorating hangover containing cricket material enzyme-decomposed with Alcalase - Google Patents

Abstract

The present invention relates to a composition containing enzyme-treated Gryllidae and Tenebrio molitor larva, and extracts of Bupleurum falcatum, Pueraria montana, Hovenia dulcis and Curcuma longa. The composition has an effect of inhibiting liver damage and can be used as a composition capable of preventing or treating various liver diseases by lowering the hepatitis index, and the alcohol decomposition effect and the acetaldehyde decomposition effect are excellent. The composition can be easily used as the composition for protecting the liver protection or easing the hangover by being taken before/after drinking by alleviating headache and vomiting symptoms of the next day after drinking.

Description

TECHNICAL FIELD The present invention relates to a composition for hepatoprotective or hangover containing cricket-enzyme-treated water,

The present invention relates to a composition for liver protection or hangover containing a cricket enzyme-treated product.

The liver is the central organ that metabolizes carbohydrates, proteins, and fats. It is an important organ responsible for the excretion of substances that enter the body through the body through oxidation, reduction, hydrolysis, and inclusion. However, in the early stages of liver disease, the pain or awareness symptoms do not appear well, and only when it has become worse, the liver is known as the 'organ of silence'.

Symptoms of worsening liver disease include constipation, irritable bowel syndrome, or color and variation of the stool. Patients with liver damage may have persistent vomiting, and sudden changes in body weight . In addition, body fluids that are impaired in the body's protein production and circulation ability due to hepatic dysfunction may be lowered to the lower body by gravity, causing cardiovascular problems such as edema of the legs, ankles and feet, and liver damage such as fatigue, chronic fatigue, Of the most common symptoms may occur.

Signs of severe liver damage include elevated levels of albumin and protein in the blood and body, resulting in swelling of the abdomen due to accumulation of fluid (ascites) in the abdomen. Suddenly, yellowish skin and eyes It is a sign of liver damage. There may also be pain in the right upper abdomen or the lower right part of the ribcage (liver pain), increased bilirubin levels in the bloodstream, which can turn the urine into a dark yellow color, and the skin may become tickle or peel off.

Causes of hepatic damage include hepatitis viruses (A, B, C), alcohol (alcohol), and nonalcoholic fatty liver, drugs or toxic hepatitis. According to the statistics of the Health Insurance Evaluation and Evaluation Agency, the cost of treatment for alcoholic liver disease in Korea is rapidly increasing every year. In addition, even if we analyze the statistics of domestic life insurers, death rate of alcoholic liver disease among hospitals has surged seven times compared with 10 years ago. Alcoholic liver disease caused by persistent alcohol consumption has become a global social problem including domestic. Especially, when alcoholic fatty liver and hepatitis persist, liver fibrosis and liver cirrhosis can no longer be expected to recover, Is the only treatment option.

According to National Cancer Center statistics, 60 ~ 70% of hepatitis virus carriers in Korea are hepatitis B carriers, and 75% of liver cancer patients are carriers of hepatitis B, and 5 ~ 10% There is also a statistic called a virus carrier. Hepatitis B virus and chronic hepatitis B are the most common malignancies in the world.

Conventional liver disease drugs are currently used as liver function supplements, antiviral agents, hepatocyte stimulators, immunosuppressants, fibrosis inhibitors, biphenyldimethyldicarboxylate and interferon. However, low therapeutic effects, high side effects, Is known to increase the side effects rather than increase the therapeutic effect. Therefore, it is necessary to study the development of functional raw materials for liver function improvement from natural substances with few side effects.

In Korea, the drinking rate of men over 20 years old is very high (83.3%) and the rate of women drinking is also rapidly increasing to 54.9%, which is seriously exposed to excessive drinking due to wrong drinking culture. The damage caused by excessive consumption of alcohol is not only a cause of various diseases such as liver disease, but also affects the emotional life and life of individual, family, and society, causing national economic and social loss.

Excessive overeating can lead to many forms of side effects, such as thirst, general boredom, fatigue, memory loss, abdominal bloating, dyspepsia, vomiting, diarrhea, and vitamin deficiency, and the risk of alcoholism do.

Alcoholic fatty liver disease, alcoholic hepatitis, and alcoholic cirrhosis are the major diseases caused by chronic alcoholism in liver disease, but it is rare that only one pure disease appears in one person. It is the most common cause of liver cirrhosis in the United States and is the second most common cause of cirrhosis after viral hepatitis in Korea.

Normal alcohol metabolism is absorbed in the stomach or small intestine when alcohol is ingested, 10% of which is excreted by breathing, sweat, urine, and the remaining 90% of the alcohol enters the blood vessel and is metabolized in the liver (M. Nakanishi Saishin Igaku, 31, p2086, 1976). Alcohol dehydrogenase (ADH), which is present in hepatocytes, oxidizes alcohol to acetaldehyde. Acetaldehyde is decomposed into acetic acid by acetaldehyde dehydrogenase in hepatocytes and transferred to muscle or fat tissue of the whole body Finally, it is decomposed into carbon dioxide and water. However, when the alcohol metabolism abnormality occurs due to the excessive metabolism, the blood alcohol level which is not metabolized is increased and acetaldehyde accumulates, causing cerebral vasoconstriction, resulting in heavy head, hangover of headache or heartburn .

On the other hand, searching for new substances that can be used as health functional foods that can prevent or treat human diseases from natural products and improve various functions has been studied by many scientists from the past. Through the development of such natural resources, more than 50% of currently used drugs are derived from natural products, and about 120 kinds of natural medicine approved by the US FDA have reached a market size of about 10 trillion dollars. Although many natural products with antitoxic activity have been searched and developed, only a small number of cases have been used as actual therapeutic agents or have undergone clinical trials. Natural products related to liver health that can be reproduced in the books of Donguibogam, natural materials and other books include apple with hepatotoxic cleansing function, broccoli with liver detoxification function, liver with protection function, shiitake with preventive effect on liver, Cabbage with liver function improving function, and tofu with hepatocyte regeneration function. It is known that sufficient protein supply is required for regeneration of injured hepatocytes. Functional raw materials that help protect the liver from alcoholic damage include milk cow extract, broccoli sprout powder, mushroom mycelium extract, bramble extract powder, and bellflower extract, Tree and bottle extracts, and fermented seaweed extracts of lactic acid bacteria (Food and Drug Administration, 2017).

Korean Patent No. 10-0477957 (a novel pyrrole derivative having a liver protecting activity isolated from Goji) and a composition containing the same, Korea Patent No. 10-0633851 A composition for prevention and treatment of liver disease or liver disease comprising an acidic garlic extract as an active ingredient), Korean Patent No. 10-1106499 (a food composition for liver protecting effect including a hinoki extract, 25 (3), p307, 2015), the effect of the recovery of toxicity (Jeongsu Jeon et al., Korean Journal of Sericultural Science, 50 (2), p93, 2012) Liver protection effect (An Mi Yeong et al., Journal of the Korean Society of Food Science and Technology, 34 (4), p684, 2002).

Korean Patent Laid-Open No. 2002-0081995 (solution of a hangover and a liver function-improving agent and a preparation method thereof), Korean Patent Publication No. 2002-0064151 (having hepatotoxicity and hangover resolution activity separated from Hovenia dulcis (Such as extracts of low alcohol-insoluble fractions and polysaccharides and compositions containing the same) have been found to have efficacy for hangover in a variety of plants such as rice ginseng extract, alder tree, oak wood vinegar, , But they are known to be effective or insignificant in some of the various changes induced by drinking.

Therefore, the development of functional foods related to liver health is advantageous for the development of a combination of natural products having effects on each factor that induces liver disease, rather than a single natural product, which helps maintain liver health, In order to do this, it is necessary to evaluate the activity of liver diseases by using many natural products.

In recent years, some of the insects that have been frequently used as herbal medicines have been recognized as food raw materials, and research into the development of functional foods based on insects has been actively conducted.

A pair of crickets ( Gryllus bimaculatus ) belongs to the cricket and is an incomplete metamorphic insect. About 800 crickets are known worldwide, and about 40 crickets are known in Korea. Crickets of each pair are recognized by the Food and Drug Administration as food ingredients. In Oriental medicine, crickets have been known to be effective in the treatment of fever, diuretic, nerve palsy, urinary incontinence, and women's dystocia. Pairs of crickets have a high protein content, and omega-3 and omega-6 fatty acids have been reported to be effective in the development of brain and dementia in young children due to the EPA component and the neurotransmitter, DHA, have. It is also known that alcohol degradation enzyme promotes alcohol metabolism and protects the liver and prevents the accumulation of anti-fatigue and lipid peroxidation in the body.

Brown duck ( Tenebrio molitor ) is a species of beetle neck and insect. The brown goose is distributed all over the world including Korea. Nutritional components of brown larva larvae are attracting attention as a food resource of the future worldwide because they are 45 ~ 57% of dry weight, 25 ~ 34% of fat and 8 ~ 11% of carbohydrate. Recently, antioxidant activity, anti-inflammatory effect of polysaccharide, toxicity to cancer cells and increase of anticancer activity have been reported in studies on pharmacological efficacy of brown goats.

Bupleurum falcatum is a perennial herbaceous plant belonging to the genus Porphyra and has an efficacy to smoothly communicate the energy of the liver and acts on liver and spleen and thus has the effect of normalizing the liver and resolving the crying. Saikosaponin D, a major component of psoriasis, inhibits liver damage by its pharmacological effect. Inhibition of urinary protein secretion, inhibition of blood cholesterol levels, increase of corticosterone in blood and adrenal gland have been reported. In addition, inhibition of VCAM-1 / VLA-4 activity and recovery of hepatic injury have been reported in extracts of Chiho, Is used to prescribe such things as Kami-on-bomb and Shiho-keolmoryotang.

Pueraria thunbergiana is a perennial plant that does not freeze in winter, but most of its stem survives. The stem is classified as a tree because it grows thicker every year and forms a thick stem. In one room, the root is used as a medicinal substance called 葛根, and it has effects such as sweating and fever. It is rich in catechin components and has the effects of liver function improvement, liver function recovery, hangover resolution and detoxification.

The Hovenia dulcis It is a deciduous tree of the Seagull. It is a good food for preventing various kinds of liver diseases. It is a liver detoxification which can show effects such as frequent fatigue, fatty liver, jaundice, hangover resolution and liver function improvement. Good food.

Curcuma longa is a plant belonging to the ginger family. Ulgum contains yellow pigment Curcumin, Turmerone, zingiberene and PTMC with bile secretion. Recently, studies on physiological activity have been reported that promote hepatic detoxification, promote bile secretion, remove bile duct stones, kink, diuretic, anti-hemorrhagic, antibiotic, anti-ulcer and blood cholesterol inhibition. Hepatitis, especially chronic type C, , Gastritis, menstrual irregularities, hypertension, arteriosclerosis, etc., anticancer and anti-diabetic. Also, it is a herb that is antioxidant effect of free oxygen which is the source of aging and panic disease.

In studying various physiological activities of various insects and plants, the inventors of the present invention found that the enzymatic treatment of the cricket, the enzyme-treated water of the brown goat larva, the myrtle extract, the myrtle extract, the myrtle extract, And thus the present invention has been completed.

Korean Patent No. 10-0477957 (entitled "Novel pyrrole Derivatives Having Liver-protecting Activity Isolated from Gugija and Compositions Containing the Same", Applicant: Seoul National University Industry-University Cooperation Foundation, Registered Date: March 10, 2005) Korean Patent No. 10-0633851 (Title of the Invention: Composition for prevention or treatment of liver diseases or liver diseases containing the heat-dried acid garlic extract as an active ingredient, Applicant: Sangji Institute of Education, Date of Registration: 2006 April 04) Korean Patent No. 10-1106499, entitled "Food Composition for Liver Protection Effect Containing Extract of Hodgkin's Thunb extract," Applicant: Jangheung Gun, Registered: January 10, 2012) Korean Patent No. 10-1852840 (Name of the invention: composition for protecting liver containing slug enzyme-treated water, applicant: Hanmi Co., Ltd., registered on Apr. 23, 2018) Korean Patent No. 10-0453575 (title of the invention: food composition containing cricket or its extract, inhibiting liver fatigue, promoting alcohol metabolism, inhibiting lipid peroxidation, applicant: Republic of Korea; Month 11th) Korean Patent Laid-Open No. 10-2002-0081995 (entitled "Hangover Elimination and Liver Function Improving Agent and Its Preparation Method by Blended Food, Applicant: Drug Co., Ltd., Disclosure Date: October 30, 2002) Korean Patent Laid-Open No. 10-2002-0064151 (entitled: Insoluble fraction of lower alcohol insoluble extract having hepatotoxicity and hangover resolution activity isolated from Hovenia dulcis and polysaccharide substance and composition containing the same, Applicant: , Publication day: August 07, 2002)

(Korean Journal of Sericultural Science, 50 (2), p93, 2012) Protection of hepatopteran and hepatocyte regeneration (Lee, EJ et al., Journal of Life Science; 25 (3), p307, 2015) (Korean Journal of Food Science and Technology, 34 (4), p684, 2002) M. Nakanishi; Saishin Igaku, 31, p2086, 1976.

It is an object of the present invention to provide a composition for liver protection and hangover containing a cricket enzyme treatment.

The present invention relates to a composition for liver protection or hangover containing a cricket enzyme-treated product, an enzyme-treated product of brown larch larvae, a chrysanthemum extract, a hornbreech extract, a chrysanthemum extract and a curd extract.

Wherein said composition comprises 20-50% by weight of a cricket enzyme-treated, 20-50% by weight of an enzyme-treated brown goose larva, 5-20% by weight of a myrtle extract, 5-20% by weight of a myrtle extract, And 5 to 20% by weight of corn oil extract.

The crickets may be adult crickets by pair.

The enzymatic treatment of the cricket-enzyme-treated or brown-larva larva is a treatment of an enzyme selected from Flavozyme, Alcalase or Protease in crickets or brown duck larva, which is a raw material sample, .

The present invention provides a composition for prevention or treatment of alcoholic liver diseases, which comprises a cricket enzyme-treated product, an enzyme-treated product of brown larch larvae, a Chrysoprase extract, a Hodgkin's extract,

The present invention also provides a health functional food for preventing or ameliorating alcoholic liver diseases, which comprises the cricket enzyme-treated product, the enzyme-treated product of brown larch larva, the chrysanthemum extract, Hovenia dulcis extract,

The alcoholic liver disease may be an alcoholic hepatitis, an alcoholic fatty liver or an alcoholic liver cirrhosis.

Hereinafter, the present invention will be described in detail.

The present invention relates to a composition for liver protection or hangover decomposition containing a cricket enzyme-treated product, an enzyme-treated product of a brown larva larva, a chrysanthemum extract, a hornbreech extract, a chrysanthemum extract and a curd extract, Can be an adult of a pair of crickets

Wherein said composition comprises 20-50% by weight of a cricket enzyme-treated, 20-50% by weight of an enzyme-treated brown goose larva, 5-20% by weight of a myrtle extract, 5-20% by weight of a myrtle extract, And 5 to 20% by weight of the extract of Ulvae. If the composition is less than 20% by weight of the treated cricket enzyme or less than 20% by weight of the enzyme treated product of the larva, the content of the useful ingredient in the composition is lowered Protection and hangover efficacy can be lowered, and even if the cricket enzyme treatment exceeds 50% by weight or the enzyme treated by brown goose larvae exceeds 50% by weight, the other ingredients are reduced in content, It may be difficult to show the synergistic effect due to the mixing of each component. If the extracts are less than 5% by weight, the activity of the extract may not be exhibited, so that the efficacy of the extract may be lowered and the efficacy of the extract may be lowered. The extracts of Seiho, Hovenia dulcis, If it exceeds 20 wt%, the bitter taste of the composition may become stronger and the taste may not be good. Also, the specific gravity of the cricket-enzyme-treated and brownish larva larvae may be lowered, and the effect of the composition on the liver protection and hangover may be lowered.

The cricket-enzyme-treated or brown-gill larva-treated enzyme is selected from the group consisting of Flavozyme, Alcalase and Protease in crickets or brown duck larva, which is a raw material sample, It is preferable to use protease N as the above protease. It is most preferable to use an alkarase as the enzyme.

The enzyme treated material of the cricket-enzyme-treated water or brown dung larvae is prepared by adding 200 to 400 parts by weight of purified water based on 100 parts by weight of crickets or brown mallow larvae as a raw material sample, heating the mixture at 100 to 150 ° C for 0.5 hours to 2 hours, ~ 60 ° C, and then enzymatically treated for 5 ~ 10 hours. At this time, it is preferable to add the enzyme in an amount of 0.1 to 10 parts by weight based on 100 parts by weight of crickets or larvae of the raw material.

After the enzyme treatment, it can be pulverized after being concentrated to have a sugar content of 20 to 30 Brix, and the enzyme can be inactivated by heat treatment at 90 to 100 ° C for 20 to 60 minutes before or after concentration.

Or the enzyme-treated liquid can be concentrated and filtered, and the oil component separated from the cricket or brown larva can be separated and removed immediately after the enzyme treatment or after concentration.

The liquid phase immediately after the enzyme treatment or after the concentration can be dried and pulverized, and a usual drying method such as spray drying, hot air drying or freeze drying can be used.

When the purified water is less than 200 parts by weight on the basis of 100 parts by weight of crickets or brown goose larvae during the above-mentioned enzyme treatment, the quality of the proteolytic composition may be deteriorated due to over-activation of the enzyme in the subsequent enzyme treatment, If it exceeds the weight part, the enzymatic activity after the subsequent enzyme treatment may be weak and the protein decomposition may not be performed well.

If the heating temperature is less than 100 ° C., the bactericidal action may not occur. If the heating temperature exceeds 150 ° C., the property of the protein may change The quality of the protein composition may deteriorate. Likewise, sterilization may be difficult even if the heating condition is less than 0.5 hour, and if the heating time exceeds 2 hours, the property of the protein may be changed.

If the enzyme treatment temperature is less than 40 캜, the enzyme activity may be hardly exhibited, and if it exceeds 60 캜, the enzyme may be inactivated and not function.

When the enzyme is added in an amount of less than 0.1 part by weight on the basis of 100 parts by weight of crickets or brown larvae in the enzyme treatment process, the enzyme activity may hardly be exhibited. When the enzyme is added in an amount exceeding 10 parts by weight, It is undesirable and the function of the enzyme may be overcome and the properties of the final proteolytic product may be worse.

At this time, the cricket or brown goat larva, which is a raw material sample, is used for fasting and discharging the excrement completely, but it is preferable to heat it after adding purified water after washing with purified water in a fresh state. After the heating process is sufficiently performed, After crushing crickets or brown larvae and enzymatically treating them, hydrolysis is preferably carried out.

The hepatoprotective effect of the present invention can be achieved by using g-GT (gamma-glutamyl phosphatase), GPT (glutamate-pyruvate trabminase), GOT (glutamate- oxaloacetate transminase), AP (alkaline phosphatase) and TBIL (total bilirubin) The hangover resolution effect may be characterized by at least one kind of hangover resolution index selected from the group consisting of blood alcohol concentration, acetaldehyde concentration, respiratory alcohol concentration and blood alcohol concentration And may be characterized by decreasing the factor or increasing the activity of the aldehyde dehydrogenase.

Each of the extracts of Seiho, Hovenia dulcifera, Lycoris spp., And Lycoris spp. Contained in the composition of the present invention can be extracted with water, C1-C4 alcohol or a mixture thereof as a solvent as a raw material sample, , The C1-C4 alcohol may be selected from the group consisting of methanol, ethanol, propanol, isopropanol, butanol and isobutanol. At this time, it is preferable to use water as a solvent.

The solvent may be used in an amount of 1 to 40 times by volume or weight, preferably 5 to 40 times by volume or weight, based on the weight of the raw material sample, such as Seiho, Houttuynia cordata, The extracting conditions of the above-mentioned Seeds, Hovenia dulcis, Lycopersicon esculentum and Lycoris spp. Can be from 20 to 100 ° C for 1 minute to 48 hours. The above process can be repeated 1 to 4 times.

As a conventional method in the art, it is also possible to dissolve water, C1-C4 alcohol or a mixed solution thereof in water, such as the above-mentioned Citrate, Hovenia dulcis, n-propanol, n-butanol, and the like.

The extracting device or the fraction thereof may be extracted by a conventional extracting device, an ultrasonic pulverizing extractor or a fractionating device. The extract or fraction thus prepared can be removed by hot air drying, vacuum drying or freeze drying. The extract or fraction may be purified by using column chromatography.

The above extract or fraction may be isolated or isolated by known methods used for separation and extraction of plant components, such as extraction with an organic solvent (acolol, ether acetone, etc.), partition with hexane and water, And may be fractionated or purified by a suitable combination method.

The chromatography was carried out using silica gel column chromatography, LH-20 column chromatography, ion exchange resin chromatography, medium pressure liquid chromatography medium pressure liquid chromatography, TLC thin layer chromatograph, silica gel vacuum liquid chromatography and high performance liquid chromatography.

Further, the present invention provides a pharmaceutical composition for preventing or treating liver disease, which comprises a cricket enzyme-treated product, an enzyme-treated product of brown larch larvae, a chrysolophyte extract, a hornbreech extract, a chrysanthemum extract, and a curd extract. The cricket enzyme-treated product, the enzyme-treated product of brown larch larva, chrysanthemum extract, Hovenia dulcis Thunb extract, chrysanthemum extract, and chrysanthemum extract may be added in an amount of 0.001 to 30% by weight to the pharmaceutical composition of the present invention.

The pharmaceutical composition may be formulated in the form of powders, granules, tablets, capsules, oral solutions, emulsions, syrups, aerosols and the like, external preparations, suppositories and sterilized injection solutions according to the usual methods . Examples of carriers, excipients and diluents that can be included in the pharmaceutical composition include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, , Methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. In the case of formulation, a diluent or excipient such as a filler, an extender, a binder, a wetting agent, a disintegrant, or a surfactant is usually used. Solid formulations for oral administration include tablets, pills, powders, granules, capsules, and the like, which may be prepared by mixing the pharmaceutical composition of the present invention with at least one excipient such as starch, calcium carbonate, sucrose or lactose , Gelatin, and the like. In addition to simple excipients, lubricants such as magnesium stearate and calc are also used. Liquid preparations for oral use include suspensions, solutions, emulsions, syrups and the like. Various excipients such as wetting agents, sweeteners, fragrances and preservatives are included in addition to water and liquid paraffin which are commonly used simple diluents. . Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Propellants, propylene glycol, polyether glycol, vegetable oils such as olive oil, injectable esters such as ethyl oleate, and the like can be used. Examples of the suppository base include withexol, macrogol, tween 61, cacao butter, laurin, glycerogelatin and the like.

The dosage of the pharmaceutical composition of the present invention will vary depending on the age, sex, body weight of the subject to be treated and the specific disease or condition to be treated, the severity of the disease or condition, the administration hardness and the judgment of the prescriber. Dosage determinations based on these factors are within the level of ordinary skill in the art and generally the dosage ranges from 0.01 mg / kg / day to approximately 2,000 mg / kg / day. A more preferred dosage is 1 mg / kg / day to 500 mg / kg / day. The administration can be carried out once a day or divided into several times. The dose is not intended to limit the scope of the invention in any way.

The pharmaceutical composition of the present invention can be administered to mammals such as rats, livestock, humans, and the like in various routes. All modes of administration may be expected, for example, by oral, rectal or intravenous, intramuscular, subcutaneous, intra-uterine dural or intracerebral injection. The composition for hepatoprotecting or hangover of the present invention has little toxicity and side effects, and thus can be safely used for long-term use for preventive purposes.

In addition, the present invention provides a liver-protecting or hangover nutritional functional food containing a pharmaceutically acceptable food-aid additive to cricket-enzyme-treated fish, an enzyme-treated brown goat larvae, Sephora, Hovenia dulcis, do.

Also provided is a health functional food for preventing or ameliorating liver disease, comprising a cricket enzyme-treated product, an enzyme-treated product of brown larch larvae, a food-grade acceptable food-aid additive to Seaweed, Hovenia dulcis,

The cricket enzyme-treated product, the enzyme-treated product of brown larch larvae, Sephora, Hovenia dulcis Thunb., Lycopersicon esculentum and Lycoris spp. May be added in an amount of 0.001 to 30% by weight to the health functional food of the present invention. The health functional food of the present invention includes forms such as tablets, capsules, pills, and liquids. Examples of foods to which the extract of the present invention can be added include various kinds of drinks, meat, sausage, bread, , Noodles, ice cream, dairy products, soups, ionic drinks, beverages, alcoholic beverages, gums, tea and vitamin complexes.

The present invention relates to a composition containing a cricket-enzyme-treated product, an enzyme-treated product of brown larch larvae, a chrysanthemum extract, a chrysanthemum extract, a hornbreech extract and a curd extract. The composition can be used as a composition capable of inhibiting liver damage and lowering the hepatitis index to prevent or treat various liver diseases. The composition has excellent alcohol decomposing effect and acetaldehyde decomposing effect and can be used for the next day's headache, And can be easily used as a composition for liver protection or hangover which is taken before / after drinking.

Korean Patent No. 10-1852840 also discloses a liver protecting composition containing an enzyme-treated product of brown goat larva. However, in the same manner as the composition of the present invention, an enzyme-treated brown crustacean larva, an enzyme treated cricket, The effects of heparinization efficacy or hangover resolution of the compositions containing Seiho Extract, Seaweed Extract, Hovenia dulcis Extract and Seaweed Extract have not been disclosed yet.

Hereinafter, preferred embodiments of the present invention will be described in detail. However, the present invention is not limited to the embodiments described herein but can be embodied in other forms. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the concept of the invention to those skilled in the art.

≪ Example 1: Preparation of each powder mixture >

Example 1-1. Preparation of enzymatically treated cricket-treated fish and brown goat larvae

The cricket larvae of 2.5 to 3.0 cm in size and the 8th instar larvae were purchased from professional breeding farms. The crickets of each pair were fasted for 1 day and the brown goat larvae were fasted for 3 days, Washed three times.

After that, the pair of cricket larvae and the brown larvae were placed in a booster, and then three times the weight of purified water was added, and the pressure was 1.2 kgf / cm < ² & The mixture was heated at a high temperature of 121 ° C for 90 minutes and then pulverized using a wet pulverizer (LSI, NEYZSCH, Srlb Bavaria, Germany) at a bead size of 0.4 mm and a rotor speed of 3,000 rpm to obtain crushed crickets The crushed material is prepared for brown larvae. When the weight of the insect before pulverization was 100 parts by weight, 1 part by weight of Alcalase (Subtilis Carlsberg, Novozymes) was added to each of the pulverized materials, and hydrolysis was carried out at 40 ° C for 5 hours. Each of the hydrolyzed hydrolyzate was concentrated to have a sugar content of 25 Brix. The inlet temperature and the outlet temperature of the spray dryer were set to 170 ° C. and 75 ° C., respectively. The spraying pressure of the spray dryer was 1.5 kgf / Was prepared at a rotation speed of 8,000 ~ 10,000 rpm, and the thus prepared powder was called an enzyme-treated product of cricket-enzyme-treated and brown larvae.

Examples 1-2. Manufacture of Seoho Extract, Seed Extract, Hovenia dulcis Extract and Ulgum Extract

Seoho, Jung, Hodeong and Ulgum were purchased from Kyungdong Market (Seoul, Jagi-dong) and used as raw materials for extraction. Each of the above extracted raw materials was charged with purified water having a weight of 10 times, extracted at 90 ° C for 12 hours, filtered and spray-dried to obtain each of the extracts in powder form.

≪ Example 2: Preparation of mixture &

Each powder sample prepared in Example 1 was mixed as shown in Table 1 below to prepare a mixed powder.

Condition The cricket enzyme-treated water powder prepared in Example 1-1 The brown goat's larva enzyme-treated powder prepared in Example 1-1 The seed extract powder prepared in Example 1-2 The powdery mildew extract powder prepared in Example 1-2 The hinoki extract powder prepared in Example 1-2 The urogine extract powder prepared in Example 1-2 Example 2-1 40 30 5 10 10 5 Example 2-2 30 40 5 10 10 5 Example 2-3 50 30 5 5 5 5 Examples 2-4 30 50 5 5 5 5 Example 2-5 20 20 10 20 20 10 Examples 2-6 20 20 20 10 10 20

≪ Comparative Example 1 > Preparation of cricket powder and brown gill larva powder without enzyme treatment [

The same cricket larvae and brown goat larvae as in Example 1-1 were pulverized, and the cricket larvae and brown goat larvae powder without enzymatic treatment were heated Respectively.

≪ Comparative Example 2: Preparation of comparative mixture >

Each powder was mixed under the conditions shown in Table 2 below.

Condition The cricket powder prepared in Comparative Example 1 The brownish larval larva powder prepared in Comparative Example 1 The cricket enzyme hydrolyzate powder prepared in Example 1-1 The brown goat's larva enzyme hydrolyzate powder prepared in Example 1-1 The syrup extract powder prepared in Example 1-2 Example 1-
2 extract powder The hinoki extract powder prepared in Example 1-2 Example 1-
2 < tb > Comparative Example 2-1 0 0 70 10 5 5 5 5 Comparative Example 2-2 0 0 10 70 5 5 5 5 Comparative Example 2-3 0 0 40 0 10 20 20 10 Comparative Example 2-4 0 0 0 40 10 20 20 10 Comparative Example 2-5 0 0 50 50 0 0 0 0 Comparative Example 2-6 40 0 0 0 10 20 20 10 Comparative Example 2-7 0 40 0 0 10 20 20 10 Comparative Example 2-8 40 30 0 0 5 10 10 5

≪ Example 3: Free amino acid analysis >

The free amino acid contents of the cricket species treated with enzymes, the enzymes treated with brown cricket larvae, the cricket crustaceans, and the brown goat larvae were compared before and after the enzyme treatment. At this time, threonine, methionine, and glutamic acid, which have been reported to have the effect of improving alcoholic liver disease, were identified in detail (Lee JH, et al, 1999, Korean J. Food Sci. Technol. 31: 802-808). Threonine helps prevent the accumulation of liver fat, methionine reduces liver fat and protects the kidneys, and glutamate is also known to help control alcoholism, schizophrenia, and sugar cravings.

For each sample preparation, 10 mL of 6 HCl was added to 1.00 g of the sample, and after decomposition with nitrogen, the sample was decomposed at 110 ° C. for 24 hours. The hydrolysis solution was concentrated under reduced pressure at 50 ° C or lower, a small amount of distilled water (10 mL) was added to the concentrate, and concentrated under reduced pressure was repeated three times to remove hydrochloric acid. After that, each concentrate was dissolved in 50 mL of 0.2N sodium citrate buffer (pH 2.2), filtered and analyzed with an amino acid analyzer.

The amino acid analyzer was S7130 amino acid reagent organizer from Sykam (Germany), S5200 sample injector and S2100 solvent delivery system. The column was analyzed using cation separation column LCA K06 / NA (4.6 x 250 mm). The flow rate of the mobile phase was 0.45 mL / min, and the concentration of ninhydrin was 0.4 mL / min.

division cricket Brown ass Before enzyme treatment After enzyme treatment Before enzyme treatment After enzyme treatment Amino acid total (mg / L) 36,680 156,166 32,182 130,271 Essential amino acids (mg / L) 10,354 44,670  8,516 44,679 Non-essential amino acids (mg / L) 26,326 111,496 23,666 85,592 Alcoholic liver disease improvement effect
Related amino acids (mg / L) Sum 7,248 27,553 7,017 40,988 Threonine 1,925 7,853 1,562 18,139 Methionine 766 3,021 203 4,310 Glutamic acid 4,556 16,679 5,558 22,849

As a result of the analysis, it was confirmed that the total amount of amino acids was increased by the enzyme treatment as shown in Table 3, and threonine, methionine, and glutamic acid were also remarkably increased.

EXPERIMENTAL EXAMPLE 1: Induction of alcohol-administered animal model and administration of substance:

Male Sprague-Dawley rats weighing 160 ~ 180g were purchased from Korea BioLink (Voice, Korea). The breeding environment was maintained at a temperature of 20 ± 2 ° C, humidity of 55 ± 1% (RH), and a light-dark cycle of 12 hours. The feed was given a fixed amount of PMI Nutrition International for a certain period of time, and the water was allowed to freely use sterilized water using a water bottle.

In the comparative group for the administration of the test materials of Example 2 and Comparative Example 2, each composition was administered instead of feed, which was prepared as granules having a hardness of 3.0 kg / cm ^{2 using} a reverse rotation granulator and then administered with the dried granules. As a control group, a solid feed (PMI Nutrition International) for experimental animals was pulverized into a 70-mesh particle size by a roll mill, and then granulated to a hardness of 3.0 kg / cm ^{2 using} a reverse-rotation granulator.

The thus prepared general feed granules and the granules prepared from the powders of Example 2 and Comparative Example 2 were fed unlimitedly to experimental animals (8 per each group).

At this time, ethanol was orally administered to a daily dose of 25 (v / v)% ethanol aqueous solution at a level of 5 mL / kg bw / day through a stomach tube for 6 weeks. That is, ethanol was not added to the new mixed powder feed for one week during the 8 weeks of feeding period, and after 6 weeks, the last 1 week was not administered. The test treatments were as shown in Table 4 below, and the results of the evaluation of the condition of the experimental animals were shown in the following Table 4.

division Administered group It's normal.
(Control group) General feed granule administration Alcohol-treated group 25% (v / v) ethanol aqueous solution and general feed granule administration
(Alcohol administration positive control group) Example 2-1 Granule administration group A granular preparation prepared from a 25% (v / v) aqueous ethanol solution and the powder of Example 2-1 Example 2-2 Granular administration group A granular preparation prepared from a 25% (v / v) aqueous ethanol solution and the powder of Example 2-2 Example 2-3 Granules administered group A granular preparation made from a 25% (v / v) aqueous ethanol solution and the powder of Example 2-3 Example 2-4 Granule administration group A granular preparation prepared from a 25% (v / v) aqueous ethanol solution and the powder of Example 2-4 Example 2-5 Granule administration group 25% (v / v) aqueous ethanol solution and granules prepared from the powder of Example 2-5 Example 2-6 Granule administration group A granular preparation prepared from a 25% (v / v) aqueous ethanol solution and the powder of Example 2-6 Comparative Example 2-1 Granule administration group Granular preparation prepared from 25% (v / v) aqueous ethanol solution and powder of Comparative Example 2-1 Comparative Example 2-2 The granule administration group A granular preparation prepared from an aqueous 25% (v / v) ethanol solution and the powder of Comparative Example 2-2 Comparative Example 2-3 Granules administered group Granular preparation prepared from 25% (v / v) aqueous ethanol solution and powder of Comparative Example 2-3 Comparative Example 2-4 Granule administration group Granular preparation prepared from 25% (v / v) aqueous ethanol solution and powder of Comparative Example 2-4 Comparative Example 2-5 Granule administration group Granule preparation prepared from 25% (v / v) aqueous ethanol solution and powder of Comparative Example 2-5 Comparative Example 2-6 Granule administration group Granule preparation prepared from 25% (v / v) aqueous ethanol solution and powder of Comparative Example 2-6 Comparative Example 2-7 Granule administration group Granular preparation prepared from 25% (v / v) aqueous ethanol solution and powder of Comparative Example 2-7 Comparative Example 2-8 Granule administration group A granular preparation prepared from a 25% (v / v) aqueous ethanol solution and a powder of Comparative Example 2-8

<Experimental Example 2: Confirmation of change in body weight>

All animals of Experimental Example 1 were subjected to symptom observation once a day. The body weight was measured by using an animal scales on a 5-day unit basis at a constant time. The granule administration group prepared from the powders of Example 2 and Comparative Example 2, The results of the granule administration group prepared from the powder of Example 2-8 are shown in Table 5.

division Start of experiment Day 5 Day 10 Day 15 Day 20 Day 25 On the 30th day It's normal. 168g 178g 213 g 228 g 231 g 242 g 253 g Alcohol-treated group 173 g 174 g 180g 192g 211g 217 g 220g Example 2-1
Granule treated group 172g 176 g 205g 217 g 230g 234 g 238 g Comparative Example 2-8
Granule treated group 174 g 175g 189 g 210g 220g 224 g 226 g

No animals died during this test, and no animals exhibited any specific clinical symptoms. Body weight was increased in all groups during the test period, but the increase rate was slower in the other dietary group than in the normal diet group, and the degree of slowing was lower in the alcohol + cricket enzyme treated diet group.

<Experimental Example 3> Blood biochemical analysis result>

After completion of the test, the blood collected from the abdominal artery was allowed to stand at room temperature for 30 minutes, centrifuged at 3,000 rpm at 4 ° C for 15 minutes to separate the serum, and the serum enzyme was analyzed with a serum automatic analyzer (Dri-ch em 2000, Fujifilm, Tokyo, Japan). Six types of serum enzymes were identified: glutamate-pyruvate (GPT), glutamate-oxaloacetate (GOT), gamma-phosphatase, alkaline phosphatase (AP), total bilirubin (TBIL) and direct bilirubin Were measured. GOT, GPT, g-GT and ALP tests are widely used for the diagnosis of liver disease. The increase of these enzymatic activity values is not only highly correlated with the extent of cell damage but also is sensitive to other blood enzymes.

Of these experiments, blood GOT and GPT levels are shown in Table 6 below.

division GOT (U / L) GPT (U / L) It's normal.
(Control group) 75.8 ± 3.6 50.9 ± 3.5 Alcohol-treated group 120.3 ± 4.5 85.2 ± 5.1 Example 2-1 Granule administration group 82.3 ± 2.8 61.9 ± 3.5 Example 2-2 Granular administration group 79.9 ± 3.4 65.7 ± 4.5 Example 2-3 Granules administered group 84.3 ± 4.2 67.8 ± 4.6 Example 2-4 Granule administration group 85.4 ± 4.5 68.6 ± 4.8 Example 2-5 Granule administration group 87.5 ± 4.9 68.1 ± 3.4 Example 2-6 Granule administration group 82.6 ± 2.3 67.0 ± 2.5 Comparative Example 2-1 Granule administration group 98.5 ± 3.9 79.2 ± 3.6 Comparative Example 2-2 The granule administration group 97.3 ± 4.9 77.0 ± 3.9 Comparative Example 2-3 Granules administered group 103.2 ± 3.5 76.6 ± 3.2 Comparative Example 2-4 Granule administration group 100.9 ± 3.9 79.0 ± 3.5 Comparative Example 2-5 Granule administration group 99.6 ± 4.8 82.9 ± 4.1 Comparative Example 2-6 Granule administration group 98.6 ± 3.6 81.4 ± 3.9 Comparative Example 2-7 Granule administration group 101.7 ± 4.8 82.7 ± 4.4 Comparative Example 2-8 Granule administration group 104.2 ± 2.1 80.5 ± 2.7

As an example of the powder administration group of Example 2-1, in the case of GOT, the granules prepared with the powder of Example 2-1 were mixed with alcohol while the general feed granule with the alcohol was administered at 120.3 U / L And 82.3 U / L for one group, respectively. In the case of GPT, the amount of granules prepared from the powder of Example 2-1 was 61.9 U / L, while that of the group fed with the general feed granules with alcohol was 85.2 U / L. Overall, the results were similar for Examples 2-1 to 2-6, and Comparative Examples 2-1 to 2-8 were similar, indicating that GOT and GPT values, as well as other values related to liver damage, show the same pattern .

From the results below, results of the granule administration group prepared from powder of Example 2-1 and the granules of powder of Comparative Example 2-8, representative of the result of granule administration group prepared from powders of Example 2 and Comparative Example 2, are presented Respectively.

Alkaline phosphatase (AP) activity is shown in Table 7 below.

division AP (U / L) It's normal. 150 Alcohol-treated group 275 Example 2-1 Granule administration group 180 Comparative Example 2-8 Granule administration group 238

In the case of AP, 12.2% in the group administered with the granules prepared from the powder of Comparative Example 2-8, the powder prepared in Example 2-1 together with the alcohol, And 31.2% in the group treated with granules.

The AP value was an index that increased as the damage / recovery cycle of the hepatocyte increased, indicating that GPT inhibition was effectively suppressed in the group administered with the granule prepared in Example 2-1 with alcohol, , It can be said that the composition of the present invention is an indicator for supporting the activity of suppressing GOT or billirubin levels.

If gamma-glutamyl phosphatase (g-GT) is elevated to a high degree, it may be a chronic cholesteatoma, and can diagnose biliary cirrhosis or sclerosing cholangitis. The results of analysis of g-GT activity are shown in Table 8.

division g-GT (U / L) It's normal. 5.9 Alcohol-treated group 6.6 Example 2-1 Granule administration group 5.2 Comparative Example 2-8 Granule administration group 6.3

g-GT was improved by 20.6% in the group fed with the granules prepared with the powder of Example 2-1 together with alcohol compared with the group fed with the general feed granules with alcohol.

When total bilirubin (TBIL) increases in the blood, it can be regarded as obstructive jaundice, which is not excreted in the liver to the bile duct. The results of analysis of TBIL activity are shown in Table 9.

division TBIL (U / L) It's normal. 0.61 Alcohol-treated group 0.75 Example 2-1 Granule administration group 0.48 Comparative Example 2-8 Granule administration group 0.67

TBIL was improved by 39% in the group fed with the granules prepared with the powder of Example 2-1 together with alcohol compared with the group fed with the general feed granules with alcohol. These results also confirm that the composition of the present invention has liver-protecting activity.

In the case of increased direct-bilirubin (DBIL), it is suspected that the hepatic parenchymal disease is not included in the liver. The results of analysis of DBIL activity are shown in Table 10.

division DBIL (U / L) It's normal. 0.18 Alcohol-treated group 0.28 Example 2-1 Granule administration group 0.12 Comparative Example 2-8 Granule administration group 0.23

DBIL was improved by 65% in the group fed with the granules prepared with the powder of Example 2-1 together with alcohol compared with the group fed with the general feed granules with alcohol.

<Experimental Example 4> Histological analysis results>

Experimental Example 1 The contents of reduced glutathione (GSH) and oxidized glutathione (GSSG) in liver were measured by cutting the right lobe of liver tissues of all animals under the condition.

The cut liver tissues were sectioned and weighed, and then 4 times of 0.1 M potassium phosphate buffer (pH 7.4) was added thereto, and the mixture was ground with a tissue mill in ice to obtain an enzyme-containing enzyme solution. Reduced glutathione content analysis was performed according to the method of Ellman (1959) and S'wiergosz-Kowaleska et al. (2006) with the enzyme solution. The total glutathione content was first analyzed and then the value obtained by subtracting the content of oxidized glutathione from this value Is taken as reduced type glutathione, and the values are shown in Table 11.

Reduced glutathione refers to the addition of SH (S is sulfur, H is hydrogen) to glutathione. These reduced glutathione functions to remove harmful free radicals. Referring to Table 11, the amount of reduced glutathione was reduced to that of the powder of Comparative Example 2-8 together with the alcohol, 11.5% in the case of one granule, and 14.5% in the case of the granules prepared with the powder of Example 2-1 together with the alcohol, thus confirming that the example composition is more effective for the antioxidative action.

division Total GSH
(mu mol) Reduced GSH
(mu mol) It's normal. 4.66 ± 0.38 3.99 ± 0.35 Alcohol-treated group 4.28 ± 0.45 3.81 ± 0.33 Example 2-1 Granule administration group 4.59 ± 0.43 3.26 ± 0.28 Comparative Example 2-8 Granule administration group 4.82 ± 0.45 3.32 ± 0.36

<Experimental Example 7> Confirmation of inflammatory and oxidative stress inhibitory effect>

Experimental Example 1 TNF-α and IL-1β, which are inflammatory cytokines, were measured in blood of all animals under the conditions. The results are shown in Table 12. Each experiment was confirmed using a kit (TNF-α IRTA800) provided by R & D systems. To briefly describe this process, the serum obtained in this experiment was reacted for 2 hours in a 96-well plate precoated with an antibody capable of binding to each cytokine present in the blood, Absorbance was confirmed at 540 ㎚ or 570 ㎚ by treating the substrate solution to determine the bound amount.

division TNF-a (pg / ml) IL-1? (Pg / ml) It's normal. 6.4 ± 0.3 130.9 ± 3.5 Alcohol-treated group 17.3 + 1.7 196.3 ± 5.0 Example 2-1 Granule administration group 8.0 ± 0.4 129.6 ± 3.1 Comparative Example 2-8 Granule administration group 14.9 ± 0.7 169.4 ± 2.6

The expression of TNF-α and IL-1β was increased in the alcohol and general feed animals, but the expression of these cytokines and markers in the granules treated with the powder of Example 2-1 was found to be normal And it was found that the production of inflammatory substances due to alcohol consumption was suppressed. On the other hand, the effect of reducing the inflammation was not good in the granule-treated group prepared with the powder of Comparative Example 2-8.

These results show that the composition of the present invention is excellent in hepatoprotective effect. Through these functions, it is possible to provide a pharmaceutical composition for preventing, improving or treating various alimentary diseases such as hepatitis and fatty liver caused by alcohol, It can be seen that it can be used as food.

<Experimental Example 8> Evaluation of alcohol decomposition efficiency>

Experimental Example 8-1. Blood alcohol reduction efficacy

In order to confirm the alcohol decomposition effect of the extract prepared in Example 2 and Comparative Example 2, alcohol was administered to the experimental animals purified in Experimental Example 1, and blood alcohol concentration and acetaldehyde concentration after 1, 3 and 5 hours were measured And the alcohol degradation effect in the body was evaluated. The number of experimental animals was 6 per group. The classification of each experimental group was as shown in Table 4.

Alcohol was administered orally through a stomach tube at a level of 3 g / kg, bw / day of ethanol (v / v) ethanol aqueous solution of 25 (v / v) ethanol. Each sample contained 500 mg / kg of granular powder for Example 2 and Comparative Example 2 Dissolved in distilled water equal to the alcohol dose, and administered twice at 30 minutes before and after alcohol administration. Blood alcohol concentration and acetaldehyde concentration were measured 1, 3, and 5 hours after administration, and the alcohol degradation effect in the body was evaluated. The blood was collected from the abdominal vein of an experimental animal, and the collected blood was centrifuged at 3000 rpm for 30 minutes, and the concentration of alcohol was measured using an ethanol measuring kit (abcam AB6543). The results are shown in Table 13.

As shown in Table 13, the alcohol concentration was decreased with time, but the degree of decrease was slow. On the other hand, in the group administered with the powder of Example 2-1 at the same time as the administration of the alcohol, the blood alcohol concentration was significantly lowered than that of the control group, particularly, 62.0 mg / L was shown at 5 hours. In addition, the compositions of the other examples showed similar effects, and it was confirmed that these compositions significantly reduced the concentrations of ethanol and acetaldehyde in blood.

As described above, it was confirmed that the composition of the present invention has an effect of maximizing the hangover resolution capability and reducing the concentration of ethanol and acetaldehyde in blood to an excellent level.

division Blood alcohol concentration
(Mg% alcohol) Blood acetaldehyde concentration
(Mg / L) 1 hours 3 hours 5 hours 1 hours 3 hours 5 hours Alcohol-treated group 205.4 ± 4.3 170.5 ± 3.9 159.3 ± 4.2 0.267 0.178 0.152 Example 2-1 Granule administration group 92.4 ± 3.9 79.4 ± 3.6 62.0 ± 3.6 0.215 0.092 0.074 Example 2-2 Granular administration group 91.2 ± 2.5 81.2 ± 2.1 55.7 ± 2.7 0.210 0.085 0.067 Example 2-3 Granules administered group 89.0 ± 4.3 75.5 ± 1.5 69.4 ± 1.3 0.207 0.106 0.066 Example 2-4 Granule administration group 95.3 ± 2.1 82.2 ± 5.3 65.8 ± 2.4 0.195 0.083 0.059 Example 2-5 Granule administration group 102.2 ± 5.3 73.0 ± 2.7 53.2 ± 3.0 0.216 0.090 0.056 Example 2-6 Granule administration group 98.3 ± 4.0 77.2 ± 3.9 63.1 ± 4.2 0.203 0.093 0.061 Comparative Example 2-1 Granule administration group 169.7 ± 4.4 158.6 ± 3.4 128.7 ± 3.9 0.236 0.146 0.129 Comparative Example 2-2 The granule administration group 171.8 ± 4.2 151.2 ± 3.5 123.7 ± 3.4 0.237 0.143 0.131 Comparative Example 2-3 Granules administered group 179.0 ± 3.4 148.4 ± 2.8 127.7 ± 3.3 0.236 0.152 0.128 Comparative Example 2-4 Granule administration group 164.1 ± 1.2 153.3 ± 2.3 134.7 ± 2.9 0.241 0.174 0.125 Comparative Example 2-5 Granule administration group 168.2 ± 2.8 149.7 ± 4.3 120.7 + 1.7 0.242 0.163 0.132 Comparative Example 2-6 Granule administration group 162.4 ± 5.1 153.0 ± 2.3 131.7 ± 2.9 0.238 0.153 0.134 Comparative Example 2-7 Granule administration group 174.5 ± 3.2 152.3 ± 1.2 138.2 ± 2.7 0.242 0.152 0.129 Comparative Example 2-8 Granule administration group 176.5 ± 2.7 159.6 ± 2.9 134.7 ± 2.6 0.239 0.142 0.127

Experimental Example 8-2. Investigation of aldehyde dehydrogenase (ALDH) activity and acetaldehyde (ADH) production

To investigate the effects of the compositions prepared in Example 2-1 and Comparative Example 2-8 on the activity of aldehyde dehydrogenase (ALDH) and the production of acetaldehyde (ADH), serum of the experimental animals obtained in Experimental Example 1 And the amount of ALDH activity and ADH was measured. ALDH activity was measured using an aldehyde dehydrogenase activity colorimetric assay kit (BioVision), and ADH was measured using an aldehyde quantification assay kit. Respectively.

Label ALDH activity (mU) ADH (mM / L) 1 hours 3 hours 5 hours 1 hours 3 hours 5 hours Alcohol-treated group 1.4 ± 0.1 1.3 ± 0.2 1.0 ± 0.2 110 ± 9.0 125 ± 10.2 140 ± 12.0 Example 2-1 Granule administration group 1.8 ± 0.2 1.7 ± 0.1 1.6 ± 0.2 94 ± 8.7 97 ± 9.8 104 ± 10.9 Comparative Example 2-8 Granule administration group 1.5 ± 0.1 1.4 ± 0.2 1.2 ± 0.1 107 ± 8.9 117 ± 10.1 130 ± 11.3

The activity of the aldehyde dehydrogenase was significantly higher in the granule-treated group prepared with the powder of Example 2-1 than in the general-feed group, and the amount of acetaldehyde was much smaller in the granule-treated group prepared from the powder of Example 2-1 .

Thus, it was found that the granules prepared from the powder of Example 2-1 of the present invention had an excellent alcohol decomposition ability.

Experimental Example 8-3. Respiratory alcohol concentration measurement

Six to each male under age 20 to 50 years old were allowed to consume 6 ml / kg / bw of commercial shochu in 25 minutes with alcohol concentration within 5 minutes, 300 mg / kg of granular powder of Example 2-1 and Comparative Example 2-8 The alcohol concentration was measured at 1, 2, and 3 hours after alcohol consumption with a breathalyzer (Breathlizer, ALT000 Korea, Inc.). The results are shown in Table 15 .

division Time of measurement (after alcohol consumption) 1 hours(%) 3 hours(%) 5 hours (%) Alcohol intake 0.086 0.04 0.067 0.03 0.052 + 0.02 Alcohol intake +
Example 2-1 Granule ingestion in powder form 0.052 ± 0.01 0.045 0.02 0.034 ± 0.01 Alcohol intake +
Comparative Example 2-8 Granule ingestion in powder form 0.077 0.03 0.062 0.02 0.047 ± 0.01

As shown in Table 15, respiratory alcohol concentration in the alcohol-only group was decreased to 0.086% in 1 hour after alcohol consumption, to 0.052% and 0.067% in Example 2-1 and Comparative Example 2-8, The respiratory alcohol concentration was 0.067% in the case of alcohol-only group at 3 hours, and 0.045% and 0.058% in the case of Example 2-1 and Comparative Example 2-8. In particular, in the composition of Example 2-1, The concentration was significantly lower.

As described above, it was confirmed that the composition of the present invention has the effect of maximizing the hangover resolution capability and remarkably reducing the respiratory alcohol concentration.

<Experimental Example 9> Confirmation of hangover solution effect>

In order to confirm that the composition of the present invention solves the hangover the day after actual drinking, the composition of the present invention is administered after drinking.

The subjects were 10 healthy adults aged 20 years or older. The subjects were divided into three groups: three pork per cup of shochu, 300 ml of shochu for 60 minutes, 4 g each of the granules prepared from the composition of Example 2 and Comparative Example 2 were ingested. Among the comparative groups, there was a bottle of Morning Care (100 ml) sold on the market, and a group taking each single composition used for preparing the composition of the present invention. These groups were classified into 5 stages (1 to 5) with 5 as 'No effect' and 5 as 'Highest effect' for the item of hangover elimination effect, digestion effect, The results are shown in Table 16 below.

Condition Hangover removal effect Degree of digestion Degree of headache reduction Example 2-1 Granules prepared from powder 4.2 4.1 4.5 Example 2-2 Granules prepared from powder 3.9 4.3 4.3 Example 2-3 Granules prepared from powder 4.3 4.0 4.3 Example 2-4 Granules prepared from powder 4.3 4.2 4.0 Example 2-5 Granules prepared from powder 4.3 4.1 4.1 Example 2-6 Granules prepared from powder 4.2 4.4 4.0 Comparative Example 2-1 Granules prepared from powder 2.8 2.8 3.2 Comparative Example 2-2 Granulated powder 2.9 3.0 3.1 Comparative Example 2-3 Granules prepared from powder 3.6 3.1 3.1 Comparative Example 2-4 Granules prepared from powder 2.4 3.4 2.6 Comparative Example 2-5 Granules prepared from powder 2.3 3.2 2.6 Comparative Example 2-6 Granules prepared from powder 2.8 2.7 2.8 Comparative Example 2-7 Granules prepared from powder 3.2 2.3 3.0 Comparative Example 2-8 Granules prepared from powder 3.3 2.4 2.9 Granules made from cricket adult powder 2.2 2.3 2.7 Granules made from brown goat larvae powder 2.3 2.6 2.5 Enzymatic treatment of adult crickets Granules made from powder 2.4 2.4 2.7 Enzymatic treatment of brown goat larva Granules prepared from water powder 2.7 2.8 2.3 Granules prepared from Seiho extract powder 2.6 2.4 2.6 Granules made from Hovenia dulcis extract powder 2.5 2.6 2.3 Granules made from extract powder 2.3 2.3 2.7 Granules made from urogulin extract powder 2.6 2.3 2.4 bottled water 1.0 1.1 1.1 Morning care 2.7 2.9 2.8

As shown in Table 16, it was confirmed that when the composition according to the present invention was used, the hangover effect was the highest.

&Lt; Formulation Example 1 >

Formulation Example 1-1. Manufacture of tablets

200 g of the powder mixture of Example 2-1 of the present invention was mixed with 175.9 g of lactose, 180 g of potato starch and 32 g of colloidal silicic acid. To this mixture was added a 10% gelatin solution, which was pulverized and passed through a 14-mesh sieve. This was dried, and a mixture obtained by adding 160 g of potato starch, 50 g of talc and 5 g of magnesium stearate was made into tablets.

<Formulation Example 2: Food Preparation>

The powder mixture of Example 2-1 of the present invention was added to the cooking sauce at 1% by weight to prepare a cooking sauce for health promotion.

Formulation Example 2-2. Manufacture of flour food products

The powder mixture of Example 2-1 of the present invention was added to wheat flour at 0.1 wt%, and bread, cake, cookies, crackers and noodles were prepared using the mixture to prepare health food.

Preparation Example 2-3. Manufacture of soups and gravies

The powder mixture of Example 2-1 of the present invention was added to soup and juice at 0.1% by weight to prepare health promotion soup and juice.

Formulation Example 2-4. Manufacture of dairy products

The powder mixture of Example 2-1 of the present invention was added to milk in an amount of 0.1% by weight and various dairy products such as butter and ice cream were prepared using the milk.

Formulation Example 2-5. Vegetable juice manufacturing

Healthy vegetable juice was prepared by adding 0.5 g of the powder mixture of Example 2-1 of the present invention to 1, 00 ml of tomato juice or carrot juice.

Formulation Example 2-6. Manufacture of fruit juice

Health enhancing fruit juice was prepared by adding 0.1 g of the powder mixture of Example 2-1 of the present invention to 1,000 ml of apple juice or grape juice.

Claims (7)

20 to 50% by weight of cricket enzyme-treated water, 20 to 50% by weight of an enzyme-treated water of brown larvae, 5 to 20% by weight of water extract, 5 to 20% by weight of water extract, 5 to 20% 5 to 20% by weight of the composition. The method according to claim 1,
Wherein said cricket is an adult of cricket by pair. The method according to claim 1,
The cricket-enzyme-treated or brown-gill larva-treated enzyme is characterized in that the cricket or brown larva, which is a raw material sample, is treated with an enzyme selected from Flavozyme, Alcalase or Protease &Lt; / RTI &gt; or a pharmaceutically acceptable salt thereof. 20 to 50% by weight of cricket enzyme-treated water, 20 to 50% by weight of an enzyme-treated water of brown larvae, 5 to 20% by weight of water extract, 5 to 20% by weight of water extract, 5 to 20% 5 to 20% by weight based on the total weight of the composition. 5. The method of claim 4,
Wherein the alcoholic liver disease is an alcoholic hepatitis, an alcoholic fatty liver or an alcoholic liver cirrhosis. 20 to 50% by weight of cricket enzyme-treated water, 20 to 50% by weight of an enzyme-treated water of brown larvae, 5 to 20% by weight of water extract, 5 to 20% by weight of water extract, 5 to 20% And 5 to 20% by weight of the composition. The method according to claim 6,
Wherein said alcoholic liver disease is alcoholic hepatitis, alcoholic fatty liver or alcoholic liver cirrhosis.